FvH4_6g12450

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011575
Physical Location & Seq
Forward (+)
7532833 .. 7535560
2728 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g12450.t2

Sequence Viewer

Length: 726 bp
ATGTCAGGAGTCGATTTAGAGCGAGGCGGAGCTGCTCAAACCCACCGCCTGTCCTTCTCCAGGAGTGACGTCAGCGGCGACGGGAGCGTCTGCTTCTCCGACGCGGACGACGGGTCGTGCTACTCGCAGTTCTTTTCGACCAACGGCGGGTCGTACGACGAGTACAACTTTGCCGCTTTTGTGTCTGATCCTGCGGAGGCCGGAGTGGTCGGGGTAACGTCGTCTTCGTCGTCCTCGGAGGAGTCTAGGAGAGACTCGTCGGTTTCGGAGTGTTCGGTCCATGTCGTGGAAATTGAGGTTGGGGCGCCGGAAATTAAGGTGCATTTGGATAGAGTAGAGAGAGATTGTCGGATTTGTCATCTGGGCTTGGAAAGTAACAGCCATGAATCCGGAGCTCCGATTGACTTGGGCTGTTCTTGCAAAGATGATCTTGGTTCGGCCCACCGGAACTGCGCTGATGCCTGGTTCAAGATCAAGGGAAACAAGATCTGCGAGATCTGCCATTCAATTGCCCGCAACATTGTTGGGCCTAATGACATTGAGATGATTGAGCAGCTGAATAATGAAGCCGCTAGTGCCTCAGCTACTGCTGCAGTCTCAGCACCAGGGCCTTCAACAGAGACTCGAAGCTTCTGGCATGGTCATCGCTTCCTCAACTTCCTTCTTGCTTGTATGGTATTTGCTTTCGTCATATCATGGCTCTTTCACTTCAATGTTCCGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

242

Amino Acids

25.89

Weight (kDa)

4.88

Isoelectric Point (pI)

52.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 116 - 162 1.2e-07 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 86
AatII GACGTC 1 cut(s) 72
AccB1I GGYRCC 1 cut(s) 304
AccB7I CCANNNNNTGG 1 cut(s) 286
AccII CGCG 1 cut(s) 104
AccIII TCCGGA 1 cut(s) 389
AciI CCGC 9 cut(s) 27, 46, 75, 104, 147, 174, 194, 514, 570
AclWI GGATC 1 cut(s) 182
AcyI GRCGYC 2 cut(s) 69, 305
AfaI GTAC 2 cut(s) 155, 164
AfiI CCNNNNNNNGG 3 cut(s) 60, 147, 286
AgsI TTSAA 4 cut(s) 469, 507, 615, 712
AhdI GACNNNNNGTC 1 cut(s) 112
AjnI CCWGG 3 cut(s) 59, 461, 604
AjuI GAANNNNNNNTTGG 2 cut(s) 282, 314
AluBI AGCT 5 cut(s) 32, 395, 556, 584, 630
AluI AGCT 5 cut(s) 32, 395, 556, 584, 630
Alw21I GWGCWC 1 cut(s) 397
Alw26I GTCTC 3 cut(s) 246, 601, 614
AlwI GGATC 1 cut(s) 182
AlwNI CAGNNNCTG 1 cut(s) 587
Aor13HI TCCGGA 1 cut(s) 389
AoxI GGCC 4 cut(s) 198, 438, 527, 608
ApeKI GCWGC 3 cut(s) 32, 553, 590
AspLEI GCGC 2 cut(s) 307, 455
AspS9I GGNCC 4 cut(s) 277, 439, 527, 608
AvaII GGWCC 1 cut(s) 277
BanI GGYRCC 1 cut(s) 304
BanII GRGCYC 1 cut(s) 397
BbsI GAAGAC 1 cut(s) 216
Bbv12I GWGCWC 1 cut(s) 397
BbvCI CCTCAGC 1 cut(s) 580
BbvI GCAGC 3 cut(s) 19, 565, 577
BceAI ACGGC 1 cut(s) 160
BcgI CGANNNNNNTGC 2 cut(s) 626, 660
BciT130I CCWGG 3 cut(s) 61, 463, 606
BcoDI GTCTC 3 cut(s) 246, 601, 614
BfaI CTAG 2 cut(s) 246, 573
BfmI CTRYAG 1 cut(s) 591
BfoI RGCGCY 1 cut(s) 308
BglII AGATCT 2 cut(s) 486, 495
BisI GCNGC 6 cut(s) 33, 76, 174, 554, 570, 591
BlsI GCNGC 6 cut(s) 34, 77, 175, 555, 571, 592
Bme1390I CCNGG 3 cut(s) 61, 463, 606
Bme18I GGWCC 1 cut(s) 277
BmeRI GACNNNNNGTC 1 cut(s) 112
BmgT120I GGNCC 4 cut(s) 277, 439, 527, 608
BmiI GGNNCC 1 cut(s) 306
BmrFI CCNGG 3 cut(s) 61, 463, 606
BmsI GCATC 1 cut(s) 448
BpiI GAAGAC 1 cut(s) 216
BpmI CTGGAG 1 cut(s) 43
Bpu10I CCTNAGC 1 cut(s) 580
BsaHI GRCGYC 2 cut(s) 69, 305
BsaJI CCNNGG 2 cut(s) 234, 605
BsaWI WCCGGW 2 cut(s) 389, 444
Bsc4I CCNNNNNNNGG 3 cut(s) 60, 147, 286
BseAI TCCGGA 1 cut(s) 389
BseBI CCWGG 3 cut(s) 61, 463, 606
BseDI CCNNGG 2 cut(s) 234, 605
BseLI CCNNNNNNNGG 3 cut(s) 60, 147, 286
BseMII CTCAG 2 cut(s) 594, 612
BseRI GAGGAG 1 cut(s) 254
BseXI GCAGC 3 cut(s) 19, 565, 577
Bsh1236I CGCG 1 cut(s) 104
BshFI GGCC 4 cut(s) 200, 440, 529, 610
BshNI GGYRCC 1 cut(s) 304
BsiHKAI GWGCWC 1 cut(s) 397
BsiSI CCGG 4 cut(s) 201, 308, 390, 445
BsiWI CGTACG 1 cut(s) 153
BslI CCNNNNNNNGG 3 cut(s) 60, 147, 286
BsmAI GTCTC 3 cut(s) 246, 601, 614
BsnI GGCC 4 cut(s) 200, 440, 529, 610
Bsp1286I GDGCHC 1 cut(s) 397
Bsp13I TCCGGA 1 cut(s) 389
Bsp143I GATC 5 cut(s) 187, 427, 471, 486, 495
BspACI CCGC 9 cut(s) 27, 46, 75, 104, 147, 174, 194, 514, 570
BspANI GGCC 4 cut(s) 200, 440, 529, 610
BspCNI CTCAG 2 cut(s) 593, 611
BspEI TCCGGA 1 cut(s) 389
BspFNI CGCG 1 cut(s) 104
BspHI TCATGA 1 cut(s) 722
BspLI GGNNCC 1 cut(s) 306
BspMAI CTGCAG 1 cut(s) 595
BspPI GGATC 1 cut(s) 182
BspT107I GGYRCC 1 cut(s) 304
BssECI CCNNGG 2 cut(s) 234, 605
BssMI GATC 5 cut(s) 187, 427, 471, 486, 495
BssNI GRCGYC 2 cut(s) 69, 305
Bst2UI CCWGG 3 cut(s) 61, 463, 606
BstACI GRCGYC 2 cut(s) 69, 305
BstC8I GCNNGC 1 cut(s) 514
BstDEI CTNAG 2 cut(s) 580, 598
BstENI CCTNNNNNAGG 1 cut(s) 58
BstFNI CGCG 1 cut(s) 104
BstH2I RGCGCY 1 cut(s) 308
BstHHI GCGC 2 cut(s) 307, 455
BstKTI GATC 5 cut(s) 190, 430, 474, 489, 498
BstMAI GTCTC 3 cut(s) 246, 601, 614
BstMBI GATC 5 cut(s) 187, 427, 471, 486, 495
BstMWI GCNNNNNNNGC 6 cut(s) 84, 417, 498, 575, 590, 599
BstNI CCWGG 3 cut(s) 61, 463, 606
BstSCI CCNGG 3 cut(s) 59, 461, 604
BstSFI CTRYAG 1 cut(s) 591
BstUI CGCG 1 cut(s) 104
BstV1I GCAGC 3 cut(s) 19, 565, 577
BstV2I GAAGAC 1 cut(s) 216
BstX2I RGATCY 2 cut(s) 486, 495
BstYI RGATCY 2 cut(s) 486, 495
BsuRI GGCC 4 cut(s) 200, 440, 529, 610
BtgZI GCGATG 1 cut(s) 629
Cac8I GCNNGC 1 cut(s) 514
CaiI CAGNNNCTG 1 cut(s) 587
CciI TCATGA 1 cut(s) 722
CfoI GCGC 2 cut(s) 307, 455
Cfr13I GGNCC 4 cut(s) 277, 439, 527, 608
CseI GACGC 2 cut(s) 76, 110
Csp6I GTAC 2 cut(s) 154, 163
CviAII CATG 5 cut(s) 281, 383, 638, 696, 723
CviQI GTAC 2 cut(s) 154, 163
DdeI CTNAG 2 cut(s) 580, 598
DinI GGCGCC 1 cut(s) 306
DpnI GATC 5 cut(s) 189, 429, 473, 488, 497
DpnII GATC 5 cut(s) 187, 427, 471, 486, 495
DrdI GACNNNNNNGTC 1 cut(s) 86
DriI GACNNNNNGTC 1 cut(s) 112
DseDI GACNNNNNNGTC 1 cut(s) 86
Eam1105I GACNNNNNGTC 1 cut(s) 112
EciI GGCGGA 1 cut(s) 42
Ecl136II GAGCTC 1 cut(s) 395
Eco24I GRGCYC 1 cut(s) 397
Eco47I GGWCC 1 cut(s) 277
Eco53kI GAGCTC 1 cut(s) 395
EcoICRI GAGCTC 1 cut(s) 395
EcoNI CCTNNNNNAGG 1 cut(s) 58
EcoO109I RGGNCCY 1 cut(s) 608
EcoRII CCWGG 3 cut(s) 59, 461, 604
EcoT38I GRGCYC 1 cut(s) 397
EgeI GGCGCC 1 cut(s) 306
EheI GGCGCC 1 cut(s) 306
FaeI CATG 5 cut(s) 284, 386, 641, 699, 726
FaiI YATR 7 cut(s) 282, 384, 639, 674, 692, 697, 724
FalI AAGNNNNNCTT 2 cut(s) 414, 446
FatI CATG 5 cut(s) 280, 382, 637, 695, 722
FauI CCCGC 2 cut(s) 140, 521
Fnu4HI GCNGC 6 cut(s) 33, 76, 174, 554, 570, 591
FriOI GRGCYC 1 cut(s) 397
Fsp4HI GCNGC 6 cut(s) 33, 76, 174, 554, 570, 591
FspBI CTAG 2 cut(s) 246, 573
GlaI GCGC 2 cut(s) 306, 454
GluI GCNGC 6 cut(s) 33, 76, 174, 554, 570, 591
GsuI CTGGAG 1 cut(s) 43
HaeII RGCGCY 1 cut(s) 308
HaeIII GGCC 4 cut(s) 200, 440, 529, 610
HapII CCGG 4 cut(s) 201, 308, 390, 445
HgaI GACGC 2 cut(s) 76, 110
HhaI GCGC 2 cut(s) 307, 455
Hin1I GRCGYC 2 cut(s) 69, 305
Hin1II CATG 5 cut(s) 284, 386, 641, 699, 726
Hin6I GCGC 2 cut(s) 305, 453
HinP1I GCGC 2 cut(s) 305, 453
HindIII AAGCTT 1 cut(s) 628
HinfI GANTC 5 cut(s) 9, 242, 254, 386, 622
HpaII CCGG 4 cut(s) 201, 308, 390, 445
Hpy188I TCNGA 6 cut(s) 100, 187, 238, 268, 351, 399
Hpy188III TCNNGA 4 cut(s) 6, 390, 469, 723
Hpy99I CGWCG 7 cut(s) 83, 104, 113, 161, 223, 232, 262
HpyAV CCTTC 3 cut(s) 64, 621, 671
HpyCH4IV ACGT 2 cut(s) 69, 218
HpyCH4V TGCA 3 cut(s) 322, 420, 593
HpyF10VI GCNNNNNNNGC 6 cut(s) 84, 417, 498, 575, 590, 599
HpyF3I CTNAG 2 cut(s) 580, 598
HpySE526I ACGT 2 cut(s) 69, 218
Hsp92I GRCGYC 2 cut(s) 69, 305
Hsp92II CATG 5 cut(s) 284, 386, 641, 699, 726
HspAI GCGC 2 cut(s) 305, 453
KasI GGCGCC 1 cut(s) 304
Kpn2I TCCGGA 1 cut(s) 389
Kzo9I GATC 5 cut(s) 187, 427, 471, 486, 495
LmnI GCTCC 4 cut(s) 29, 84, 392, 400
Lsp1109I GCAGC 3 cut(s) 19, 565, 577
LweI GCATC 1 cut(s) 448
MaeI CTAG 2 cut(s) 246, 573
MaeII ACGT 2 cut(s) 69, 218
MaeIII GTNAC 3 cut(s) 65, 214, 374
MalI GATC 5 cut(s) 189, 429, 473, 488, 497
MboI GATC 5 cut(s) 187, 427, 471, 486, 495
MboII GAAGA 1 cut(s) 216
MfeI CAATTG 1 cut(s) 507
MflI RGATCY 2 cut(s) 486, 495
MhlI GDGCHC 1 cut(s) 397
MluCI AATT 3 cut(s) 291, 312, 507
Mly113I GGCGCC 1 cut(s) 305
MlyI GAGTC 4 cut(s) 18, 248, 251, 616
MmeI TCCRAC 2 cut(s) 123, 329
MnlI CCTC 7 cut(s) 17, 190, 232, 244, 289, 589, 662
MroI TCCGGA 1 cut(s) 389
MseI TTAA 1 cut(s) 315
MslI CAYNNNNRTG 2 cut(s) 542, 711
MspA1I CMGCKG 2 cut(s) 75, 556
MspI CCGG 4 cut(s) 201, 308, 390, 445
MspR9I CCNGG 3 cut(s) 61, 463, 606
MunI CAATTG 1 cut(s) 507
MvaI CCWGG 3 cut(s) 61, 463, 606
MvnI CGCG 1 cut(s) 104
MwoI GCNNNNNNNGC 6 cut(s) 84, 417, 498, 575, 590, 599
NarI GGCGCC 1 cut(s) 305
NdeII GATC 5 cut(s) 187, 427, 471, 486, 495
NlaIII CATG 5 cut(s) 284, 386, 641, 699, 726
NlaIV GGNNCC 1 cut(s) 306
NmuCI GTSAC 1 cut(s) 65
PagI TCATGA 1 cut(s) 722
PcsI WCGNNNNNNNCGW 3 cut(s) 75, 224, 227
PfeI GAWTC 1 cut(s) 386
Pfl23II CGTACG 1 cut(s) 153
PflMI CCANNNNNTGG 1 cut(s) 286
PfoI TCCNGGA 1 cut(s) 59
PkrI GCNGC 6 cut(s) 34, 77, 175, 555, 571, 592
PleI GAGTC 4 cut(s) 17, 248, 250, 616
PluTI GGCGCC 1 cut(s) 308
PpsI GAGTC 4 cut(s) 17, 248, 250, 616
Psp124BI GAGCTC 1 cut(s) 397
Psp6I CCWGG 3 cut(s) 59, 461, 604
PspGI CCWGG 3 cut(s) 59, 461, 604
PspLI CGTACG 1 cut(s) 153
PspN4I GGNNCC 1 cut(s) 306
PspPI GGNCC 4 cut(s) 277, 439, 527, 608
PstI CTGCAG 1 cut(s) 595
PstNI CAGNNNCTG 1 cut(s) 587
PsuI RGATCY 2 cut(s) 486, 495
PvuII CAGCTG 1 cut(s) 556
RsaI GTAC 2 cut(s) 155, 164
RsaNI GTAC 2 cut(s) 154, 163
RseI CAYNNNNRTG 2 cut(s) 542, 711
SacI GAGCTC 1 cut(s) 397
SaqAI TTAA 1 cut(s) 315
SatI GCNGC 6 cut(s) 33, 76, 174, 554, 570, 591
Sau3AI GATC 5 cut(s) 187, 427, 471, 486, 495
Sau96I GGNCC 4 cut(s) 277, 439, 527, 608
SchI GAGTC 4 cut(s) 18, 248, 251, 616
ScrFI CCNGG 3 cut(s) 61, 463, 606
SduI GDGCHC 1 cut(s) 397
SetI ASST 9 cut(s) 34, 72, 221, 300, 321, 397, 558, 586, 632
SfaNI GCATC 1 cut(s) 448
SfcI CTRYAG 1 cut(s) 591
SfoI GGCGCC 1 cut(s) 306
SinI GGWCC 1 cut(s) 277
SmiMI CAYNNNNRTG 2 cut(s) 542, 711
Sse9I AATT 3 cut(s) 291, 312, 507
SsiI CCGC 9 cut(s) 27, 46, 75, 104, 147, 174, 194, 514, 570
SspDI GGCGCC 1 cut(s) 304
SspMI CTAG 2 cut(s) 246, 573
SstI GAGCTC 1 cut(s) 397
StyD4I CCNGG 3 cut(s) 59, 461, 604
TaiI ACGT 2 cut(s) 72, 221
TaqI TCGA 3 cut(s) 12, 137, 625
TaqII GACCGA 1 cut(s) 265
TasI AATT 3 cut(s) 291, 312, 507
TatI WGTACW 1 cut(s) 162
TauI GCSGC 3 cut(s) 78, 176, 572
TfiI GAWTC 1 cut(s) 386
Tru1I TTAA 1 cut(s) 315
Tru9I TTAA 1 cut(s) 315
TseFI GTSAC 1 cut(s) 65
TseI GCWGC 3 cut(s) 32, 553, 590
Tsp45I GTSAC 1 cut(s) 65
TspDTI ATGAA 2 cut(s) 399, 579
TspGWI ACGGA 1 cut(s) 708
Van91I CCANNNNNTGG 1 cut(s) 286
VpaK11BI GGWCC 1 cut(s) 277
XagI CCTNNNNNAGG 1 cut(s) 58
XspI CTAG 2 cut(s) 246, 573
ZraI GACGTC 1 cut(s) 70
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.