MD12G1156700.v1.1

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011575
Physical Location & Seq
Reverse (-)
23727755 .. 23731086
3332 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1156700.v1.1.491

Sequence Viewer

Length: 675 bp
ATGTCCCAAGTCGATTTAGAGCAAGGGCCCCACCGCCGCTCCTTGTCCGGAAGCGCCGACGGCGGAAGCAGCGTCTGCTTCTCTGACGCAGACGACGGGTCGTGTTACTCTCAGTTCTTCTCGACCAATGGCGGCTCCTACGACGAGTACAATTTTGCGTTCGTCTCTGATCCTCAGATCGGCGTGGTGTCAGATTCTCGGAGAGCCTCGTCTGTTTCGGACAGTTCGGTCGAGGTTGTCGAAATTGAGATTGGAGTTCCGGAAATTAAGGTGCATTTGGATAGAGTAGAGAGAGATTGTCGGATTTGTCATCTGGGTTTGGAGAGTAACAGCCACGAATCCGGGGCTCCGATTGAATTGGGTTGTTCTTGCAAGGATGATTTGGCTGCTGCCCATAAGAACTGTGCTGATACGTGGTTCAAGATTAAGGGCAACAAGACCTGTGAGATATGCCAGGCAGTTGCACGCAACGTCATTGGGCCAAATGACATCGAGTTCACAGAGCAGTCGAACGAATCCAGTGCCGCCTCAGCAACAGCTTCAGTTGCCGCCCCAGGGCCGTCCACAGAGACTCAAAGTTTCTGGCACGGTCACCGCTTCCTCAACTTCCTCCTTGCCTGCATGGTATTTGCTTTTGTCATATCATGGCTCTTTCACTTCAACGTTCCGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

225

Amino Acids

24.2

Weight (kDa)

4.76

Isoelectric Point (pI)

54.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 100 - 151 2.1e-10 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 227
AccBSI CCGCTC 1 cut(s) 39
AccIII TCCGGA 2 cut(s) 47, 259
AciI CCGC 7 cut(s) 34, 37, 63, 132, 525, 549, 595
AclI AACGTT 1 cut(s) 663
AclWI GGATC 1 cut(s) 164
AcuI CTGAAG 1 cut(s) 525
AfaI GTAC 1 cut(s) 149
AfiI CCNNNNNNNGG 2 cut(s) 179, 555
AgsI TTSAA 3 cut(s) 356, 421, 661
AhdI GACNNNNNGTC 1 cut(s) 97
AjnI CCWGG 2 cut(s) 453, 553
AjuI GAANNNNNNNTTGG 2 cut(s) 234, 266
AluBI AGCT 1 cut(s) 539
AluI AGCT 1 cut(s) 539
Alw26I GTCTC 2 cut(s) 169, 563
AlwI GGATC 1 cut(s) 164
AlwNI CAGNNNCTG 1 cut(s) 75
Aor13HI TCCGGA 2 cut(s) 47, 259
AoxI GGCC 3 cut(s) 26, 479, 557
ApaI GGGCCC 1 cut(s) 30
ApeKI GCWGC 3 cut(s) 69, 386, 389
AspLEI GCGC 1 cut(s) 56
AspS9I GGNCC 4 cut(s) 26, 27, 479, 557
AsuC2I CCSGG 1 cut(s) 343
AsuHPI GGTGA 1 cut(s) 584
BaeGI GKGCMC 1 cut(s) 30
BanII GRGCYC 2 cut(s) 30, 349
BbvCI CCTCAGC 1 cut(s) 529
BbvI GCAGC 3 cut(s) 81, 373, 376
BceAI ACGGC 2 cut(s) 76, 544
BcgI CGANNNNNNTGC 2 cut(s) 503, 537
BciT130I CCWGG 2 cut(s) 455, 555
BcnI CCSGG 1 cut(s) 343
BcoDI GTCTC 2 cut(s) 169, 563
BfoI RGCGCY 1 cut(s) 57
BisI GCNGC 7 cut(s) 37, 70, 133, 387, 390, 525, 549
BlsI GCNGC 7 cut(s) 38, 71, 134, 388, 391, 526, 550
Bme1390I CCNGG 3 cut(s) 343, 455, 555
BmeRI GACNNNNNGTC 1 cut(s) 97
BmgT120I GGNCC 4 cut(s) 26, 27, 479, 557
BmiI GGNNCC 4 cut(s) 28, 29, 136, 348
BmrFI CCNGG 3 cut(s) 343, 455, 555
Bpu10I CCTNAGC 1 cut(s) 529
BpuMI CCSGG 1 cut(s) 343
BsaAI YACGTR 1 cut(s) 414
BsaJI CCNNGG 3 cut(s) 342, 553, 554
BsaWI WCCGGW 2 cut(s) 47, 259
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bsc4I CCNNNNNNNGG 2 cut(s) 179, 555
Bse1I ACTGG 1 cut(s) 519
BseAI TCCGGA 2 cut(s) 47, 259
BseBI CCWGG 2 cut(s) 455, 555
BseDI CCNNGG 3 cut(s) 342, 553, 554
BseGI GGATG 1 cut(s) 382
BseLI CCNNNNNNNGG 2 cut(s) 179, 555
BseMII CTCAG 3 cut(s) 125, 188, 543
BseNI ACTGG 1 cut(s) 519
BseSI GKGCMC 1 cut(s) 30
BseXI GCAGC 3 cut(s) 81, 373, 376
Bsh1285I CGRYCG 1 cut(s) 231
BshFI GGCC 3 cut(s) 28, 481, 559
BsiEI CGRYCG 1 cut(s) 231
BsiSI CCGG 3 cut(s) 48, 260, 342
BslI CCNNNNNNNGG 2 cut(s) 179, 555
BsmAI GTCTC 2 cut(s) 169, 563
BsmBI CGTCTC 1 cut(s) 169
BsnI GGCC 3 cut(s) 28, 481, 559
Bsp120I GGGCCC 1 cut(s) 26
Bsp1286I GDGCHC 2 cut(s) 30, 349
Bsp13I TCCGGA 2 cut(s) 47, 259
Bsp143I GATC 2 cut(s) 169, 177
BspACI CCGC 7 cut(s) 34, 37, 63, 132, 525, 549, 595
BspANI GGCC 3 cut(s) 28, 481, 559
BspCNI CTCAG 3 cut(s) 124, 187, 542
BspEI TCCGGA 2 cut(s) 47, 259
BspLI GGNNCC 4 cut(s) 28, 29, 136, 348
BspPI GGATC 1 cut(s) 164
BsrBI CCGCTC 1 cut(s) 39
BsrI ACTGG 1 cut(s) 519
BssECI CCNNGG 3 cut(s) 342, 553, 554
BssMI GATC 2 cut(s) 169, 177
Bst2UI CCWGG 2 cut(s) 455, 555
Bst4CI ACNGT 3 cut(s) 224, 404, 590
BstAPI GCANNNNNTGC 1 cut(s) 75
BstBAI YACGTR 1 cut(s) 414
BstC8I GCNNGC 2 cut(s) 466, 619
BstDEI CTNAG 3 cut(s) 111, 174, 529
BstEII GGTNACC 1 cut(s) 590
BstF5I GGATG 1 cut(s) 382
BstH2I RGCGCY 1 cut(s) 57
BstHHI GCGC 1 cut(s) 56
BstKTI GATC 2 cut(s) 172, 180
BstMAI GTCTC 2 cut(s) 169, 563
BstMBI GATC 2 cut(s) 169, 177
BstMCI CGRYCG 1 cut(s) 231
BstMWI GCNNNNNNNGC 5 cut(s) 60, 69, 75, 530, 545
BstNI CCWGG 2 cut(s) 455, 555
BstPI GGTNACC 1 cut(s) 590
BstSCI CCNGG 3 cut(s) 341, 453, 553
BstSLI GKGCMC 1 cut(s) 30
BstV1I GCAGC 3 cut(s) 81, 373, 376
BsuRI GGCC 3 cut(s) 28, 481, 559
BtsCI GGATG 1 cut(s) 382
BtsIMutI CAGTG 1 cut(s) 526
Cac8I GCNNGC 2 cut(s) 466, 619
CaiI CAGNNNCTG 1 cut(s) 75
CfoI GCGC 1 cut(s) 56
Cfr13I GGNCC 4 cut(s) 26, 27, 479, 557
CseI GACGC 2 cut(s) 61, 95
Csp6I GTAC 1 cut(s) 148
CviAII CATG 2 cut(s) 622, 645
CviQI GTAC 1 cut(s) 148
DdeI CTNAG 3 cut(s) 111, 174, 529
DpnI GATC 2 cut(s) 171, 179
DpnII GATC 2 cut(s) 169, 177
DrdI GACNNNNNNGTC 1 cut(s) 227
DriI GACNNNNNGTC 1 cut(s) 97
DseDI GACNNNNNNGTC 1 cut(s) 227
Eam1105I GACNNNNNGTC 1 cut(s) 97
EciI GGCGGA 1 cut(s) 78
Eco24I GRGCYC 2 cut(s) 30, 349
Eco57I CTGAAG 1 cut(s) 525
Eco91I GGTNACC 1 cut(s) 590
EcoO109I RGGNCCY 2 cut(s) 26, 27
EcoO65I GGTNACC 1 cut(s) 590
EcoRII CCWGG 2 cut(s) 453, 553
EcoT38I GRGCYC 2 cut(s) 30, 349
Esp3I CGTCTC 1 cut(s) 169
FaeI CATG 2 cut(s) 625, 648
FaiI YATR 5 cut(s) 396, 451, 623, 641, 646
FatI CATG 2 cut(s) 621, 644
Fnu4HI GCNGC 7 cut(s) 37, 70, 133, 387, 390, 525, 549
FokI GGATG 1 cut(s) 389
FriOI GRGCYC 2 cut(s) 30, 349
Fsp4HI GCNGC 7 cut(s) 37, 70, 133, 387, 390, 525, 549
GlaI GCGC 1 cut(s) 55
GluI GCNGC 7 cut(s) 37, 70, 133, 387, 390, 525, 549
HaeII RGCGCY 1 cut(s) 57
HaeIII GGCC 3 cut(s) 28, 481, 559
HapII CCGG 3 cut(s) 48, 260, 342
HgaI GACGC 2 cut(s) 61, 95
HhaI GCGC 1 cut(s) 56
Hin1II CATG 2 cut(s) 625, 648
Hin6I GCGC 1 cut(s) 54
HinP1I GCGC 1 cut(s) 54
HinfI GANTC 4 cut(s) 194, 338, 515, 571
HpaII CCGG 3 cut(s) 48, 260, 342
HphI GGTGA 1 cut(s) 584
Hpy166II GTNNAC 2 cut(s) 498, 564
Hpy188I TCNGA 8 cut(s) 85, 169, 177, 193, 201, 220, 303, 351
Hpy188III TCNNGA 5 cut(s) 48, 121, 260, 421, 672
Hpy8I GTNNAC 2 cut(s) 498, 564
Hpy99I CGWCG 4 cut(s) 62, 98, 146, 673
HpyCH4III ACNGT 3 cut(s) 224, 404, 590
HpyCH4IV ACGT 3 cut(s) 413, 471, 663
HpyCH4V TGCA 4 cut(s) 274, 372, 464, 621
HpyF10VI GCNNNNNNNGC 5 cut(s) 60, 69, 75, 530, 545
HpyF3I CTNAG 3 cut(s) 111, 174, 529
HpySE526I ACGT 3 cut(s) 413, 471, 663
Hsp92II CATG 2 cut(s) 625, 648
HspAI GCGC 1 cut(s) 54
Kpn2I TCCGGA 2 cut(s) 47, 259
Kzo9I GATC 2 cut(s) 169, 177
LmnI GCTCC 3 cut(s) 44, 140, 352
Lsp1109I GCAGC 3 cut(s) 81, 373, 376
MaeII ACGT 3 cut(s) 413, 471, 663
MaeIII GTNAC 3 cut(s) 104, 326, 590
MalI GATC 2 cut(s) 171, 179
MbiI CCGCTC 1 cut(s) 39
MboI GATC 2 cut(s) 169, 177
MboII GAAGA 1 cut(s) 109
MhlI GDGCHC 2 cut(s) 30, 349
MluCI AATT 4 cut(s) 151, 243, 264, 356
MlyI GAGTC 1 cut(s) 565
MmeI TCCRAC 1 cut(s) 281
MnlI CCTC 6 cut(s) 183, 217, 226, 538, 611, 620
MroI TCCGGA 2 cut(s) 47, 259
MseI TTAA 2 cut(s) 267, 426
MspI CCGG 3 cut(s) 48, 260, 342
MspR9I CCNGG 3 cut(s) 343, 455, 555
MvaI CCWGG 2 cut(s) 455, 555
MwoI GCNNNNNNNGC 5 cut(s) 60, 69, 75, 530, 545
NciI CCSGG 1 cut(s) 343
NdeII GATC 2 cut(s) 169, 177
NlaIII CATG 2 cut(s) 625, 648
NlaIV GGNNCC 4 cut(s) 28, 29, 136, 348
NmuCI GTSAC 1 cut(s) 590
PasI CCCWGGG 1 cut(s) 554
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 3 cut(s) 194, 338, 515
PkrI GCNGC 7 cut(s) 38, 71, 134, 388, 391, 526, 550
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
Ppu21I YACGTR 1 cut(s) 414
Psp1406I AACGTT 1 cut(s) 663
Psp6I CCWGG 2 cut(s) 453, 553
PspEI GGTNACC 1 cut(s) 590
PspGI CCWGG 2 cut(s) 453, 553
PspN4I GGNNCC 4 cut(s) 28, 29, 136, 348
PspOMI GGGCCC 1 cut(s) 26
PspPI GGNCC 4 cut(s) 26, 27, 479, 557
PstNI CAGNNNCTG 1 cut(s) 75
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
SaqAI TTAA 2 cut(s) 267, 426
SatI GCNGC 7 cut(s) 37, 70, 133, 387, 390, 525, 549
Sau3AI GATC 2 cut(s) 169, 177
Sau96I GGNCC 4 cut(s) 26, 27, 479, 557
SchI GAGTC 1 cut(s) 565
ScrFI CCNGG 3 cut(s) 343, 455, 555
SduI GDGCHC 2 cut(s) 30, 349
SetI ASST 7 cut(s) 237, 273, 416, 443, 474, 541, 666
Sse9I AATT 4 cut(s) 151, 243, 264, 356
SsiI CCGC 7 cut(s) 34, 37, 63, 132, 525, 549, 595
StyD4I CCNGG 3 cut(s) 341, 453, 553
TaaI ACNGT 3 cut(s) 224, 404, 590
TaiI ACGT 3 cut(s) 416, 474, 666
TaqI TCGA 6 cut(s) 12, 122, 231, 240, 492, 509
TaqII GACCGA 1 cut(s) 217
TasI AATT 4 cut(s) 151, 243, 264, 356
TatI WGTACW 1 cut(s) 147
TauI GCSGC 4 cut(s) 39, 135, 527, 551
TfiI GAWTC 3 cut(s) 194, 338, 515
Tru1I TTAA 2 cut(s) 267, 426
Tru9I TTAA 2 cut(s) 267, 426
TscAI CASTG 1 cut(s) 526
TseFI GTSAC 1 cut(s) 590
TseI GCWGC 3 cut(s) 69, 386, 389
Tsp45I GTSAC 1 cut(s) 590
TspGWI ACGGA 1 cut(s) 657
TspRI CASTG 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.