MD04G1142100.v1.1

The RING-variant domain is a C4HC3 zinc-finger like motif found in a number of cellular and viral proteins. Some of these proteins have been shown both in vivo and in vitro to have ubiquitin E3 ligase activity.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011575
Physical Location & Seq
Reverse (-)
23098408 .. 23101290
2883 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1142100.v1.1.491

Sequence Viewer

Length: 675 bp
ATGTCCCAAGTCGATTTAGAGCAAGGAACCCACCGCCGCTCCTTCTCCGGAAGCGCCGACGGCGGCGGCAGCGTCTGCTTCTCCGACGCAGACGACGGGTCGTGTTACTCTCAGTTCTTCTCGACCAATGGCGGCTCCAACGATGAGTACAATTTCGCTTTTGTCTCTGATCCTCAGCTCGGCGTGGTGTCGGATTCTCGGAGAGCCTCGTCTGTTTCGGACAGTTCGGTCGAGGTTGTCGAAATTGAGATTGGGGTGCCGGAAATTAAGGTTCATTTGGATAGAGTAGAGAGAGATTGTCGGATTTGTCATCTGGGTTTGGAGAGTAACAGCCACGAATCCGGAGCTCCGATTGAATTGGGTTGTTCTTGCAAGGATGATTTGGCTGCTGCCCACAAGAACTGTGCTGATACGTGGTTCAAGATTAGGGGCAACAAGACCTGTGAGATATGCCAGTCAGTTGCGCGCAACGTCGTTGGGCCAAATGACATCGAGTTCACTGAGCAGTCAAACGAATCCAGCACTGCCTCAGCAACAGCTGCAGTTGCAGCCCCAGGGGCCTCCACAGAGACTCGAAGTTTCTGGCACGGTCACCGCTTCCTCAACTTCCTTCTTGCCTGCATGGTATTTGCTTTTGTCATATCATGGCTCTTTCACTTCAACGTTCCGTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RINGv

Protein Analysis

225

Amino Acids

24.21

Weight (kDa)

4.83

Isoelectric Point (pI)

54.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RINGv PF12906 100 - 151 3e-10 RING-variant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 227
AccB1I GGYRCC 1 cut(s) 256
AccBSI CCGCTC 1 cut(s) 39
AccII CGCG 1 cut(s) 466
AccIII TCCGGA 2 cut(s) 47, 341
AciI CCGC 6 cut(s) 34, 37, 63, 66, 132, 595
AclI AACGTT 1 cut(s) 663
AclWI GGATC 1 cut(s) 164
AfaI GTAC 1 cut(s) 149
AfiI CCNNNNNNNGG 1 cut(s) 179
AgsI TTSAA 3 cut(s) 356, 421, 661
AhdI GACNNNNNGTC 1 cut(s) 97
AjnI CCWGG 1 cut(s) 553
AjuI GAANNNNNNNTTGG 2 cut(s) 234, 266
AluBI AGCT 3 cut(s) 178, 347, 539
AluI AGCT 3 cut(s) 178, 347, 539
Alw21I GWGCWC 1 cut(s) 349
Alw26I GTCTC 2 cut(s) 169, 563
AlwI GGATC 1 cut(s) 164
AlwNI CAGNNNCTG 1 cut(s) 75
Aor13HI TCCGGA 2 cut(s) 47, 341
AoxI GGCC 2 cut(s) 479, 558
ApeKI GCWGC 5 cut(s) 69, 386, 389, 539, 548
AspLEI GCGC 3 cut(s) 56, 466, 468
AspS9I GGNCC 2 cut(s) 479, 558
AsuHPI GGTGA 1 cut(s) 584
BanI GGYRCC 1 cut(s) 256
BanII GRGCYC 1 cut(s) 349
Bbv12I GWGCWC 1 cut(s) 349
BbvCI CCTCAGC 2 cut(s) 174, 529
BbvI GCAGC 5 cut(s) 81, 373, 376, 526, 560
BceAI ACGGC 1 cut(s) 76
BciT130I CCWGG 1 cut(s) 555
BcoDI GTCTC 2 cut(s) 169, 563
BfmI CTRYAG 1 cut(s) 540
BfoI RGCGCY 1 cut(s) 57
BglI GCCNNNNNGGC 1 cut(s) 557
BisI GCNGC 9 cut(s) 37, 64, 67, 70, 133, 387, 390, 540, 549
BlsI GCNGC 9 cut(s) 38, 65, 68, 71, 134, 388, 391, 541, 550
Bme1390I CCNGG 1 cut(s) 555
BmeRI GACNNNNNGTC 1 cut(s) 97
BmgT120I GGNCC 2 cut(s) 479, 558
BmiI GGNNCC 4 cut(s) 28, 136, 258, 559
BmrFI CCNGG 1 cut(s) 555
Bpu10I CCTNAGC 2 cut(s) 174, 529
BsaAI YACGTR 1 cut(s) 414
BsaJI CCNNGG 2 cut(s) 553, 554
BsaWI WCCGGW 2 cut(s) 47, 341
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bsc4I CCNNNNNNNGG 1 cut(s) 179
Bse1I ACTGG 1 cut(s) 454
BseAI TCCGGA 2 cut(s) 47, 341
BseBI CCWGG 1 cut(s) 555
BseDI CCNNGG 2 cut(s) 553, 554
BseGI GGATG 1 cut(s) 382
BseLI CCNNNNNNNGG 1 cut(s) 179
BseMII CTCAG 4 cut(s) 125, 188, 492, 543
BseNI ACTGG 1 cut(s) 454
BsePI GCGCGC 1 cut(s) 464
BseXI GCAGC 5 cut(s) 81, 373, 376, 526, 560
Bsh1236I CGCG 1 cut(s) 466
Bsh1285I CGRYCG 1 cut(s) 231
BshFI GGCC 2 cut(s) 481, 560
BshNI GGYRCC 1 cut(s) 256
BsiEI CGRYCG 1 cut(s) 231
BsiHKAI GWGCWC 1 cut(s) 349
BsiSI CCGG 3 cut(s) 48, 260, 342
BslI CCNNNNNNNGG 1 cut(s) 179
BsmAI GTCTC 2 cut(s) 169, 563
BsnI GGCC 2 cut(s) 481, 560
Bsp1286I GDGCHC 1 cut(s) 349
Bsp13I TCCGGA 2 cut(s) 47, 341
Bsp143I GATC 1 cut(s) 169
BspACI CCGC 6 cut(s) 34, 37, 63, 66, 132, 595
BspANI GGCC 2 cut(s) 481, 560
BspCNI CTCAG 4 cut(s) 124, 187, 493, 542
BspEI TCCGGA 2 cut(s) 47, 341
BspFNI CGCG 1 cut(s) 466
BspLI GGNNCC 4 cut(s) 28, 136, 258, 559
BspMAI CTGCAG 1 cut(s) 544
BspPI GGATC 1 cut(s) 164
BspT107I GGYRCC 1 cut(s) 256
BsrBI CCGCTC 1 cut(s) 39
BsrI ACTGG 1 cut(s) 454
BssECI CCNNGG 2 cut(s) 553, 554
BssHII GCGCGC 1 cut(s) 464
BssMI GATC 1 cut(s) 169
Bst2UI CCWGG 1 cut(s) 555
Bst4CI ACNGT 3 cut(s) 224, 404, 590
BstAPI GCANNNNNTGC 2 cut(s) 75, 539
BstBAI YACGTR 1 cut(s) 414
BstC8I GCNNGC 2 cut(s) 466, 619
BstDEI CTNAG 4 cut(s) 111, 174, 501, 529
BstEII GGTNACC 1 cut(s) 590
BstF5I GGATG 1 cut(s) 382
BstFNI CGCG 1 cut(s) 466
BstH2I RGCGCY 1 cut(s) 57
BstHHI GCGC 3 cut(s) 56, 466, 468
BstKTI GATC 1 cut(s) 172
BstMAI GTCTC 2 cut(s) 169, 563
BstMBI GATC 1 cut(s) 169
BstMCI CGRYCG 1 cut(s) 231
BstMWI GCNNNNNNNGC 7 cut(s) 60, 69, 75, 539, 545, 548, 557
BstNI CCWGG 1 cut(s) 555
BstPI GGTNACC 1 cut(s) 590
BstSCI CCNGG 1 cut(s) 553
BstSFI CTRYAG 1 cut(s) 540
BstUI CGCG 1 cut(s) 466
BstV1I GCAGC 5 cut(s) 81, 373, 376, 526, 560
BsuRI GGCC 2 cut(s) 481, 560
BtsCI GGATG 1 cut(s) 382
BtsI GCAGTG 1 cut(s) 522
BtsIMutI CAGTG 2 cut(s) 498, 522
Cac8I GCNNGC 2 cut(s) 466, 619
CaiI CAGNNNCTG 1 cut(s) 75
CfoI GCGC 3 cut(s) 56, 466, 468
Cfr13I GGNCC 2 cut(s) 479, 558
CseI GACGC 2 cut(s) 61, 95
Csp6I GTAC 1 cut(s) 148
CviAII CATG 2 cut(s) 622, 645
CviQI GTAC 1 cut(s) 148
DdeI CTNAG 4 cut(s) 111, 174, 501, 529
DpnI GATC 1 cut(s) 171
DpnII GATC 1 cut(s) 169
DrdI GACNNNNNNGTC 1 cut(s) 227
DriI GACNNNNNGTC 1 cut(s) 97
DseDI GACNNNNNNGTC 1 cut(s) 227
Eam1105I GACNNNNNGTC 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 347
Eco24I GRGCYC 1 cut(s) 349
Eco53kI GAGCTC 1 cut(s) 347
Eco91I GGTNACC 1 cut(s) 590
EcoICRI GAGCTC 1 cut(s) 347
EcoO109I RGGNCCY 1 cut(s) 558
EcoO65I GGTNACC 1 cut(s) 590
EcoRII CCWGG 1 cut(s) 553
EcoT38I GRGCYC 1 cut(s) 349
FaeI CATG 2 cut(s) 625, 648
FaiI YATR 4 cut(s) 451, 623, 641, 646
FatI CATG 2 cut(s) 621, 644
Fnu4HI GCNGC 9 cut(s) 37, 64, 67, 70, 133, 387, 390, 540, 549
FokI GGATG 1 cut(s) 389
FriOI GRGCYC 1 cut(s) 349
Fsp4HI GCNGC 9 cut(s) 37, 64, 67, 70, 133, 387, 390, 540, 549
GlaI GCGC 3 cut(s) 55, 465, 467
GluI GCNGC 9 cut(s) 37, 64, 67, 70, 133, 387, 390, 540, 549
HaeII RGCGCY 1 cut(s) 57
HaeIII GGCC 2 cut(s) 481, 560
HapII CCGG 3 cut(s) 48, 260, 342
HgaI GACGC 2 cut(s) 61, 95
HhaI GCGC 3 cut(s) 56, 466, 468
Hin1II CATG 2 cut(s) 625, 648
Hin6I GCGC 3 cut(s) 54, 464, 466
HinP1I GCGC 3 cut(s) 54, 464, 466
HinfI GANTC 4 cut(s) 194, 338, 515, 571
HpaII CCGG 3 cut(s) 48, 260, 342
HphI GGTGA 1 cut(s) 584
Hpy166II GTNNAC 1 cut(s) 498
Hpy188I TCNGA 7 cut(s) 85, 169, 193, 201, 220, 303, 351
Hpy188III TCNNGA 5 cut(s) 48, 121, 342, 421, 672
Hpy8I GTNNAC 1 cut(s) 498
Hpy99I CGWCG 5 cut(s) 62, 89, 98, 476, 673
HpyAV CCTTC 2 cut(s) 52, 620
HpyCH4III ACNGT 3 cut(s) 224, 404, 590
HpyCH4IV ACGT 3 cut(s) 413, 471, 663
HpyCH4V TGCA 4 cut(s) 372, 542, 548, 621
HpyF10VI GCNNNNNNNGC 7 cut(s) 60, 69, 75, 539, 545, 548, 557
HpyF3I CTNAG 4 cut(s) 111, 174, 501, 529
HpySE526I ACGT 3 cut(s) 413, 471, 663
Hsp92II CATG 2 cut(s) 625, 648
HspAI GCGC 3 cut(s) 54, 464, 466
Kpn2I TCCGGA 2 cut(s) 47, 341
Kzo9I GATC 1 cut(s) 169
LmnI GCTCC 4 cut(s) 44, 140, 344, 352
Lsp1109I GCAGC 5 cut(s) 81, 373, 376, 526, 560
MaeII ACGT 3 cut(s) 413, 471, 663
MaeIII GTNAC 3 cut(s) 104, 326, 590
MalI GATC 1 cut(s) 171
MbiI CCGCTC 1 cut(s) 39
MboI GATC 1 cut(s) 169
MboII GAAGA 1 cut(s) 109
MhlI GDGCHC 1 cut(s) 349
MluCI AATT 4 cut(s) 151, 243, 264, 356
MlyI GAGTC 1 cut(s) 565
MmeI TCCRAC 4 cut(s) 108, 162, 171, 281
MnlI CCTC 6 cut(s) 183, 217, 226, 538, 571, 611
MroI TCCGGA 2 cut(s) 47, 341
MseI TTAA 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 539
MspI CCGG 3 cut(s) 48, 260, 342
MspR9I CCNGG 1 cut(s) 555
MvaI CCWGG 1 cut(s) 555
MvnI CGCG 1 cut(s) 466
MwoI GCNNNNNNNGC 7 cut(s) 60, 69, 75, 539, 545, 548, 557
NdeII GATC 1 cut(s) 169
NlaIII CATG 2 cut(s) 625, 648
NlaIV GGNNCC 4 cut(s) 28, 136, 258, 559
NmeAIII GCCGAG 1 cut(s) 159
NmuCI GTSAC 1 cut(s) 590
PasI CCCWGGG 1 cut(s) 554
PauI GCGCGC 1 cut(s) 464
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PfeI GAWTC 3 cut(s) 194, 338, 515
PkrI GCNGC 9 cut(s) 38, 65, 68, 71, 134, 388, 391, 541, 550
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
Ppu21I YACGTR 1 cut(s) 414
Psp124BI GAGCTC 1 cut(s) 349
Psp1406I AACGTT 1 cut(s) 663
Psp6I CCWGG 1 cut(s) 553
PspEI GGTNACC 1 cut(s) 590
PspGI CCWGG 1 cut(s) 553
PspN4I GGNNCC 4 cut(s) 28, 136, 258, 559
PspPI GGNCC 2 cut(s) 479, 558
PstI CTGCAG 1 cut(s) 544
PstNI CAGNNNCTG 1 cut(s) 75
PteI GCGCGC 1 cut(s) 464
PvuII CAGCTG 1 cut(s) 539
RsaI GTAC 1 cut(s) 149
RsaNI GTAC 1 cut(s) 148
SacI GAGCTC 1 cut(s) 349
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 9 cut(s) 37, 64, 67, 70, 133, 387, 390, 540, 549
Sau3AI GATC 1 cut(s) 169
Sau96I GGNCC 2 cut(s) 479, 558
SchI GAGTC 1 cut(s) 565
ScrFI CCNGG 1 cut(s) 555
SduI GDGCHC 1 cut(s) 349
SetI ASST 9 cut(s) 180, 237, 273, 349, 416, 443, 474, 541, 666
SfcI CTRYAG 1 cut(s) 540
Sse9I AATT 4 cut(s) 151, 243, 264, 356
SsiI CCGC 6 cut(s) 34, 37, 63, 66, 132, 595
SstI GAGCTC 1 cut(s) 349
StyD4I CCNGG 1 cut(s) 553
TaaI ACNGT 3 cut(s) 224, 404, 590
TaiI ACGT 3 cut(s) 416, 474, 666
TaqI TCGA 6 cut(s) 12, 122, 231, 240, 492, 574
TaqII GACCGA 1 cut(s) 217
TasI AATT 4 cut(s) 151, 243, 264, 356
TatI WGTACW 1 cut(s) 147
TauI GCSGC 4 cut(s) 39, 66, 69, 135
TfiI GAWTC 3 cut(s) 194, 338, 515
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 2 cut(s) 505, 529
TseFI GTSAC 1 cut(s) 590
TseI GCWGC 5 cut(s) 69, 386, 389, 539, 548
Tsp45I GTSAC 1 cut(s) 590
TspDTI ATGAA 1 cut(s) 263
TspGWI ACGGA 1 cut(s) 657
TspRI CASTG 2 cut(s) 505, 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.