FvH4_6g16550

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011840
Physical Location & Seq
Forward (+)
10484767 .. 10488183
3417 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g16550.t5

Sequence Viewer

Length: 882 bp
ATGTCTCTCTTACTCTTCTTCCTCCTAACTCTTTTGGTACCTTCAAGCAATGCTGGCTGGCCACCATCACCTGGCTACTGGCCAAGTTCTAAATTCAGGACTATGAGCTTCTATAAAGGGTTTAGAACCCTTTGGGGTGCTCAGCATCAGAGCTTAGACCAAAATGCATTAACAATCTGGCTTGACAGAACCACAGGAAGTGGATTCAAGTCGGTTAAACCATTTAGATCCGGTTACTTTGGTTCCTCCATTAAGCTTCAGCCTGGTTATACTGCAGGAGTTATAACAGCTTTCTATCTTTCAAACAGTGAAGCTCATCCAGGGTATCATGATGAAGTGGATATTGAATTTCTGGGGACTACATTTGGAAAGCCCTATACTTTACAGACCAATGTTTACATCAGAGGAAGTGGAGATGGAAAAATTATTGGAAGAGAGATGAAGTTTCATCTGTGGTTTGATCCTACCAAAAATTTTCATCACTATGCTATATTATGGAGTCCTAAGGAGATTATATTTTTGGTGGATGATGTGCCCATAAGGAGGTACCAAAGGAAAAGTGTTGCAACATTTCCCTTGAGGCCAATGTGGCTCTATGGTTCAATATGGGATGCCTCATCATGGGCTACTGAAGATGGAAAATACAAAGCAGATTACAGATACCAACCTTTTGTTGCGAAGTTTACCAATTTCAAAGCTGGTGGTTGCTCAGCCTATTCCCCTGCCTGGTGCCACCCGGTCTCCGCCTCTCCATTCCGGTCAGGTGGGTTGACCCGACAACAATACCGGGTCATGAAATGGGTTCAAGCCAACCATATGGTGTATGACTATTGCAGGGATTACAAGAGGGACCATTCCTTAACACCTGAATGTTGGGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008152 GO:0009056 GO:0009987 GO:0016998 GO:0043170 GO:0044036 GO:0044237 GO:0044248 GO:0044260 GO:0071554 GO:0071704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

294

Amino Acids

33.73

Weight (kDa)

9.46

Isoelectric Point (pI)

48.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 38 - 220 5.3e-55 Glycosyl hydrolases family 16
XET_C PF06955 255 - 291 1.2e-13 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011840)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36870
fragaria_vesca FvH4_6g16550 FvH4_6g16550 FvH4_6g16550 FvH4_6g16550 FvH4_6g16550
malus_domestica MD04G1091200.v1.1 MD12G1109400.v1.1
prunus_persica Prupe.7G128000_v2.0.a1
pyrus_communis pycom04g08620
rosa_chinensis RchiOBHm_Chr3g0470461
rosa_laevigata RLG00000024264
rosa_multiflora Rmu_sc0002115.1_g000003 Rmu_sc0003381.1_g000010
rosa_roxburghii Rroxscaffold_6G00410780
rosa_rugosa Rorug03G0109300
rosa_samantha Rh3AG159700 Rh3BG184200
rosa_wichuraiana Rw3G015110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 284
Acc65I GGTACC 2 cut(s) 37, 546
AccB1I GGYRCC 3 cut(s) 37, 546, 729
AccB7I CCANNNNNTGG 1 cut(s) 71
AciI CCGC 1 cut(s) 744
AclWI GGATC 2 cut(s) 222, 455
AcoI YGGCCR 2 cut(s) 59, 80
AcsI RAATTY 3 cut(s) 92, 347, 472
AcuI CTGAAG 2 cut(s) 242, 651
AfaI GTAC 2 cut(s) 39, 548
AfiI CCNNNNNNNGG 4 cut(s) 71, 543, 621, 726
AgsI TTSAA 7 cut(s) 45, 208, 303, 347, 603, 694, 806
AjnI CCWGG 4 cut(s) 70, 262, 319, 725
AluBI AGCT 6 cut(s) 108, 153, 256, 290, 314, 698
AluI AGCT 6 cut(s) 108, 153, 256, 290, 314, 698
Alw21I GWGCWC 1 cut(s) 142
Alw26I GTCTC 2 cut(s) 9, 745
AlwI GGATC 2 cut(s) 222, 455
AoxI GGCC 3 cut(s) 59, 80, 581
ApoI RAATTY 3 cut(s) 92, 347, 472
Asp718I GGTACC 2 cut(s) 37, 546
AspS9I GGNCC 1 cut(s) 850
AsuC2I CCSGG 2 cut(s) 737, 788
AsuHPI GGTGA 1 cut(s) 60
AvaII GGWCC 1 cut(s) 850
AxyI CCTNAGG 1 cut(s) 504
BaeGI GKGCMC 1 cut(s) 537
BalI TGGCCA 2 cut(s) 61, 82
BanI GGYRCC 3 cut(s) 37, 546, 729
Bbv12I GWGCWC 1 cut(s) 142
BccI CCATC 3 cut(s) 73, 410, 629
BciT130I CCWGG 4 cut(s) 72, 264, 321, 727
BcnI CCSGG 2 cut(s) 737, 788
BcoDI GTCTC 2 cut(s) 9, 745
BfmI CTRYAG 1 cut(s) 273
BglI GCCNNNNNGGC 1 cut(s) 589
BlpI GCTNAGC 2 cut(s) 141, 709
Bme1390I CCNGG 6 cut(s) 72, 264, 321, 727, 737, 788
Bme18I GGWCC 1 cut(s) 850
BmgT120I GGNCC 1 cut(s) 850
BmiI GGNNCC 5 cut(s) 39, 244, 548, 731, 851
BmrFI CCNGG 6 cut(s) 72, 264, 321, 727, 737, 788
BmsI GCATC 2 cut(s) 154, 601
Bpu1102I GCTNAGC 2 cut(s) 141, 709
BpuEI CTTGAG 1 cut(s) 598
BpuMI CCSGG 2 cut(s) 737, 788
BsaI GGTCTC 1 cut(s) 745
BsaJI CCNNGG 1 cut(s) 320
BsaWI WCCGGW 2 cut(s) 230, 756
BsaXI ACNNNNNCTCC 2 cut(s) 725, 755
Bsc4I CCNNNNNNNGG 4 cut(s) 71, 543, 621, 726
Bse1I ACTGG 1 cut(s) 83
Bse21I CCTNAGG 1 cut(s) 504
Bse3DI GCAATG 1 cut(s) 55
BseBI CCWGG 4 cut(s) 72, 264, 321, 727
BseDI CCNNGG 1 cut(s) 320
BseGI GGATG 3 cut(s) 316, 532, 616
BseLI CCNNNNNNNGG 4 cut(s) 71, 543, 621, 726
BseMI GCAATG 1 cut(s) 55
BseMII CTCAG 2 cut(s) 155, 723
BseNI ACTGG 1 cut(s) 83
BseSI GKGCMC 1 cut(s) 537
BshFI GGCC 3 cut(s) 61, 82, 583
BshNI GGYRCC 3 cut(s) 37, 546, 729
BsiHKAI GWGCWC 1 cut(s) 142
BsiSI CCGG 4 cut(s) 231, 737, 757, 787
BslFI GGGAC 2 cut(s) 370, 863
BslI CCNNNNNNNGG 4 cut(s) 71, 543, 621, 726
BsmAI GTCTC 2 cut(s) 9, 745
BsmFI GGGAC 2 cut(s) 370, 863
BsnI GGCC 3 cut(s) 61, 82, 583
Bso31I GGTCTC 1 cut(s) 745
Bsp1286I GDGCHC 2 cut(s) 142, 537
Bsp143I GATC 2 cut(s) 227, 460
Bsp1720I GCTNAGC 2 cut(s) 141, 709
BspACI CCGC 1 cut(s) 744
BspANI GGCC 3 cut(s) 61, 82, 583
BspCNI CTCAG 2 cut(s) 154, 722
BspHI TCATGA 2 cut(s) 328, 792
BspLI GGNNCC 5 cut(s) 39, 244, 548, 731, 851
BspMAI CTGCAG 1 cut(s) 277
BspPI GGATC 2 cut(s) 222, 455
BspT107I GGYRCC 3 cut(s) 37, 546, 729
BspTNI GGTCTC 1 cut(s) 745
BsrDI GCAATG 1 cut(s) 55
BsrI ACTGG 1 cut(s) 83
BssECI CCNNGG 1 cut(s) 320
BssMI GATC 2 cut(s) 227, 460
Bst2UI CCWGG 4 cut(s) 72, 264, 321, 727
Bst4CI ACNGT 1 cut(s) 308
Bst6I CTCTTC 2 cut(s) 20, 427
BstC8I GCNNGC 2 cut(s) 55, 59
BstDEI CTNAG 4 cut(s) 141, 154, 504, 709
BstF5I GGATG 3 cut(s) 316, 532, 616
BstKTI GATC 2 cut(s) 230, 463
BstMAI GTCTC 2 cut(s) 9, 745
BstMBI GATC 2 cut(s) 227, 460
BstMWI GCNNNNNNNGC 2 cut(s) 54, 589
BstNI CCWGG 4 cut(s) 72, 264, 321, 727
BstSCI CCNGG 6 cut(s) 70, 262, 319, 725, 735, 786
BstSFI CTRYAG 1 cut(s) 273
BstSLI GKGCMC 1 cut(s) 537
BstX2I RGATCY 1 cut(s) 227
BstXI CCANNNNNNTGG 1 cut(s) 817
BstYI RGATCY 1 cut(s) 227
Bsu36I CCTNAGG 1 cut(s) 504
BsuRI GGCC 3 cut(s) 61, 82, 583
BtsCI GGATG 3 cut(s) 316, 532, 616
BtsIMutI CAGTG 1 cut(s) 313
Cac8I GCNNGC 2 cut(s) 55, 59
CciI TCATGA 2 cut(s) 328, 792
Cfr13I GGNCC 1 cut(s) 850
Csp6I GTAC 2 cut(s) 38, 547
CspCI CAANNNNNGTGG 2 cut(s) 682, 717
CviAII CATG 3 cut(s) 329, 621, 793
CviQI GTAC 2 cut(s) 38, 547
DdeI CTNAG 4 cut(s) 141, 154, 504, 709
DpnI GATC 2 cut(s) 229, 462
DpnII GATC 2 cut(s) 227, 460
EaeI YGGCCR 2 cut(s) 59, 80
Eam1104I CTCTTC 2 cut(s) 20, 427
EarI CTCTTC 2 cut(s) 20, 427
EciI GGCGGA 1 cut(s) 733
Eco31I GGTCTC 1 cut(s) 745
Eco47I GGWCC 1 cut(s) 850
Eco57I CTGAAG 2 cut(s) 242, 651
Eco81I CCTNAGG 1 cut(s) 504
EcoRII CCWGG 4 cut(s) 70, 262, 319, 725
EcoT22I ATGCAT 1 cut(s) 169
FaeI CATG 3 cut(s) 332, 624, 796
FaqI GGGAC 2 cut(s) 370, 863
FatI CATG 3 cut(s) 328, 620, 792
FauNDI CATATG 1 cut(s) 816
FokI GGATG 3 cut(s) 303, 539, 623
HaeIII GGCC 3 cut(s) 61, 82, 583
HapII CCGG 4 cut(s) 231, 737, 757, 787
Hin1II CATG 3 cut(s) 332, 624, 796
HincII GTYRAC 1 cut(s) 771
HindII GTYRAC 1 cut(s) 771
HindIII AAGCTT 1 cut(s) 254
HinfI GANTC 2 cut(s) 204, 499
HpaII CCGG 4 cut(s) 231, 737, 757, 787
HphI GGTGA 1 cut(s) 60
Hpy166II GTNNAC 3 cut(s) 397, 684, 771
Hpy188I TCNGA 2 cut(s) 150, 404
Hpy188III TCNNGA 3 cut(s) 97, 329, 793
Hpy8I GTNNAC 3 cut(s) 397, 684, 771
HpyAV CCTTC 1 cut(s) 51
HpyCH4III ACNGT 1 cut(s) 308
HpyCH4V TGCA 4 cut(s) 167, 275, 566, 834
HpyF10VI GCNNNNNNNGC 2 cut(s) 54, 589
HpyF3I CTNAG 4 cut(s) 141, 154, 504, 709
Hsp92II CATG 3 cut(s) 332, 624, 796
KpnI GGTACC 2 cut(s) 41, 550
Kzo9I GATC 2 cut(s) 227, 460
LweI GCATC 2 cut(s) 154, 601
MaeIII GTNAC 1 cut(s) 233
MalI GATC 2 cut(s) 229, 462
MboI GATC 2 cut(s) 227, 460
MboII GAAGA 4 cut(s) 7, 10, 444, 644
MflI RGATCY 1 cut(s) 227
MhlI GDGCHC 2 cut(s) 142, 537
MlsI TGGCCA 2 cut(s) 61, 82
MluCI AATT 5 cut(s) 92, 347, 423, 472, 688
MluNI TGGCCA 2 cut(s) 61, 82
MlyI GAGTC 1 cut(s) 508
MnlI CCTC 8 cut(s) 32, 256, 398, 537, 573, 625, 757, 840
Mox20I TGGCCA 2 cut(s) 61, 82
Mph1103I ATGCAT 1 cut(s) 169
MscI TGGCCA 2 cut(s) 61, 82
MseI TTAA 5 cut(s) 170, 216, 252, 860, 880
MslI CAYNNNNRTG 2 cut(s) 483, 868
Msp20I TGGCCA 2 cut(s) 61, 82
MspI CCGG 4 cut(s) 231, 737, 757, 787
MspR9I CCNGG 6 cut(s) 72, 264, 321, 727, 737, 788
MvaI CCWGG 4 cut(s) 72, 264, 321, 727
MwoI GCNNNNNNNGC 2 cut(s) 54, 589
NciI CCSGG 2 cut(s) 737, 788
NdeI CATATG 1 cut(s) 816
NdeII GATC 2 cut(s) 227, 460
NlaIII CATG 3 cut(s) 332, 624, 796
NlaIV GGNNCC 5 cut(s) 39, 244, 548, 731, 851
NsiI ATGCAT 1 cut(s) 169
PagI TCATGA 2 cut(s) 328, 792
PfeI GAWTC 1 cut(s) 204
PflMI CCANNNNNTGG 1 cut(s) 71
PleI GAGTC 1 cut(s) 507
PpsI GAGTC 1 cut(s) 507
PsiI TTATAA 1 cut(s) 284
Psp6I CCWGG 4 cut(s) 70, 262, 319, 725
PspGI CCWGG 4 cut(s) 70, 262, 319, 725
PspN4I GGNNCC 5 cut(s) 39, 244, 548, 731, 851
PspPI GGNCC 1 cut(s) 850
PstI CTGCAG 1 cut(s) 277
PsuI RGATCY 1 cut(s) 227
RsaI GTAC 2 cut(s) 39, 548
RsaNI GTAC 2 cut(s) 38, 547
RseI CAYNNNNRTG 2 cut(s) 483, 868
SaqAI TTAA 5 cut(s) 170, 216, 252, 860, 880
Sau3AI GATC 2 cut(s) 227, 460
Sau96I GGNCC 1 cut(s) 850
SchI GAGTC 1 cut(s) 508
ScrFI CCNGG 6 cut(s) 72, 264, 321, 727, 737, 788
SduI GDGCHC 2 cut(s) 142, 537
SfaNI GCATC 2 cut(s) 154, 601
SfcI CTRYAG 1 cut(s) 273
SinI GGWCC 1 cut(s) 850
SmiMI CAYNNNNRTG 2 cut(s) 483, 868
SmlI CTYRAG 1 cut(s) 577
SmoI CTYRAG 1 cut(s) 577
Sse9I AATT 5 cut(s) 92, 347, 423, 472, 688
SsiI CCGC 1 cut(s) 744
StyD4I CCNGG 6 cut(s) 70, 262, 319, 725, 735, 786
TaaI ACNGT 1 cut(s) 308
TasI AATT 5 cut(s) 92, 347, 423, 472, 688
TfiI GAWTC 1 cut(s) 204
Tru1I TTAA 5 cut(s) 170, 216, 252, 860, 880
Tru9I TTAA 5 cut(s) 170, 216, 252, 860, 880
TscAI CASTG 1 cut(s) 313
TspDTI ATGAA 5 cut(s) 348, 437, 455, 467, 809
TspRI CASTG 1 cut(s) 313
Van91I CCANNNNNTGG 1 cut(s) 71
VpaK11BI GGWCC 1 cut(s) 850
XapI RAATTY 3 cut(s) 92, 347, 472
Zsp2I ATGCAT 1 cut(s) 169
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.