MD04G1091200.v1.1

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0011840
Physical Location & Seq
Reverse (-)
16271641 .. 16274175
2535 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1091200.v1.1.491

Sequence Viewer

Length: 879 bp
ATGGCTATCTTCATCTACCTCCTAATTCTTTTAGTCCCTGCAACCAATGCTGATTGGCCACCATCGCCTGGCTACTGGCCAAGCTCTAAGTTCAGGTCTATGAGCTTTAATAAAGGATTCAAACCTCTCTGGGGTCCTCAGCATCAAAGCTTATACCAAAATGCATTAACAATCTGGCTTGACAGAACCACAGGAAGTGGGTTCAAATCAGTTCGTCCATTTAGATCCGGTTACTTCGGTGCCTCCATTAAGCTTCAAACTGGTTACACAGCAGGAGTCATAACAGCTTTCTATCTTTCAAACAGTGAAGCTCATCCTGGGTACCATGATGAAGTGGACATTGAGTTTCTTGGGACTACATTTGGGAAGCCGTATACTTTGCAGACCAATGTTTACATCAGAGGAAGTGGGGATGGAAGAATTATTGGCAGAGAGATGAAGTTTCACTTGTGGTTTGATCCCAGTAAAAATTTCCATCACTATGCTATATTATGGAGTCCCAAGGAGATCATCTTTTTGGTGGATGATGTGCCCATAAGGAGGTATGCTAGGAAAAGTGTTGCAACGTTTCCCTTAAGGCCAATGTGGCTTTATGGTTCAATTTGGGATGCTTCATCTTGGGCAACTGAAGATGGAAAATACAAAGCTGATTACAGATATCAACCATTTGTTGCAAAGTATACCAATTTCAAAGCCGGTGGTTGCTCTGCCTATTCCCCCGCTTGGTGCCGCCCGGTGTCTGCCTCTCCCTTCCGGTCCGGTGGGCTAACGCGACAGCAGTACAGGACCATGAGATGGGTTCAACGCAACCATTTGGTATATGACTATTGCGGGGACCCCAAGAGGGACCATTCCAAAACACCCGAGTGTTGGGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008152 GO:0009056 GO:0009987 GO:0016998 GO:0043170 GO:0044036 GO:0044237 GO:0044248 GO:0044260 GO:0071554 GO:0071704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_16 XET_C

Protein Analysis

293

Amino Acids

33.62

Weight (kDa)

9.57

Isoelectric Point (pI)

49.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 37 - 219 1.2e-54 Glycosyl hydrolases family 16
XET_C PF06955 254 - 290 2.2e-13 Xyloglucan endo-transglycosylase (XET) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011840)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36870
fragaria_vesca FvH4_6g16550 FvH4_6g16550 FvH4_6g16550 FvH4_6g16550 FvH4_6g16550
malus_domestica MD04G1091200.v1.1 MD12G1109400.v1.1
prunus_persica Prupe.7G128000_v2.0.a1
pyrus_communis pycom04g08620
rosa_chinensis RchiOBHm_Chr3g0470461
rosa_laevigata RLG00000024264
rosa_multiflora Rmu_sc0002115.1_g000003 Rmu_sc0003381.1_g000010
rosa_roxburghii Rroxscaffold_6G00410780
rosa_rugosa Rorug03G0109300
rosa_samantha Rh3AG159700 Rh3BG184200
rosa_wichuraiana Rw3G015110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 321
AccB1I GGYRCC 3 cut(s) 239, 321, 726
AccB7I CCANNNNNTGG 2 cut(s) 68, 795
AccI GTMKAC 2 cut(s) 374, 680
AccII CGCG 1 cut(s) 772
AciI CCGC 3 cut(s) 720, 730, 831
AclI AACGTT 1 cut(s) 566
AclWI GGATC 2 cut(s) 219, 452
AcoI YGGCCR 2 cut(s) 56, 77
AcsI RAATTY 1 cut(s) 469
AcuI CTGAAG 1 cut(s) 648
AfaI GTAC 2 cut(s) 323, 782
AfiI CCNNNNNNNGG 7 cut(s) 68, 131, 540, 723, 795, 844, 870
AflII CTTAAG 1 cut(s) 574
AgsI TTSAA 7 cut(s) 121, 205, 257, 300, 600, 691, 803
AjnI CCWGG 2 cut(s) 67, 316
AleI CACNNNNGTG 1 cut(s) 865
AluBI AGCT 7 cut(s) 84, 105, 150, 253, 287, 311, 647
AluI AGCT 7 cut(s) 84, 105, 150, 253, 287, 311, 647
AlwI GGATC 2 cut(s) 219, 452
Ama87I CYCGRG 1 cut(s) 863
AoxI GGCC 3 cut(s) 56, 77, 578
ApoI RAATTY 1 cut(s) 469
Asp718I GGTACC 1 cut(s) 321
AspS9I GGNCC 5 cut(s) 134, 756, 786, 835, 847
AsuC2I CCSGG 1 cut(s) 734
AvaI CYCGRG 1 cut(s) 863
AvaII GGWCC 5 cut(s) 134, 756, 786, 835, 847
BaeGI GKGCMC 1 cut(s) 534
BalI TGGCCA 2 cut(s) 58, 79
BanI GGYRCC 3 cut(s) 239, 321, 726
BbvCI CCTCAGC 1 cut(s) 138
BccI CCATC 5 cut(s) 70, 407, 483, 626, 789
BceAI ACGGC 1 cut(s) 355
BciT130I CCWGG 2 cut(s) 69, 318
BcnI CCSGG 1 cut(s) 734
BfaI CTAG 1 cut(s) 549
BfrI CTTAAG 1 cut(s) 574
BglI GCCNNNNNGGC 1 cut(s) 586
BisI GCNGC 1 cut(s) 730
BlsI GCNGC 1 cut(s) 731
Bme1390I CCNGG 3 cut(s) 69, 318, 734
Bme18I GGWCC 5 cut(s) 134, 756, 786, 835, 847
BmeT110I CYCGRG 1 cut(s) 863
BmgT120I GGNCC 5 cut(s) 134, 756, 786, 835, 847
BmiI GGNNCC 7 cut(s) 135, 241, 323, 728, 836, 837, 848
BmrFI CCNGG 3 cut(s) 69, 318, 734
BmrI ACTGGG 1 cut(s) 456
BmsI GCATC 2 cut(s) 151, 598
BmuI ACTGGG 1 cut(s) 456
Bpu10I CCTNAGC 1 cut(s) 138
BpuMI CCSGG 1 cut(s) 734
BsaJI CCNNGG 2 cut(s) 317, 501
BsaWI WCCGGW 3 cut(s) 227, 753, 758
Bsc4I CCNNNNNNNGG 7 cut(s) 68, 131, 540, 723, 795, 844, 870
Bse118I RCCGGY 1 cut(s) 695
Bse1I ACTGG 3 cut(s) 80, 265, 462
BseBI CCWGG 2 cut(s) 69, 318
BseDI CCNNGG 2 cut(s) 317, 501
BseGI GGATG 4 cut(s) 313, 418, 529, 613
BseLI CCNNNNNNNGG 7 cut(s) 68, 131, 540, 723, 795, 844, 870
BseMII CTCAG 1 cut(s) 152
BseNI ACTGG 3 cut(s) 80, 265, 462
BseSI GKGCMC 1 cut(s) 534
Bsh1236I CGCG 1 cut(s) 772
BshFI GGCC 3 cut(s) 58, 79, 580
BshNI GGYRCC 3 cut(s) 239, 321, 726
BsiHKCI CYCGRG 1 cut(s) 863
BsiSI CCGG 5 cut(s) 228, 696, 734, 754, 759
BslFI GGGAC 5 cut(s) 20, 367, 483, 848, 860
BslI CCNNNNNNNGG 7 cut(s) 68, 131, 540, 723, 795, 844, 870
BsmFI GGGAC 5 cut(s) 20, 367, 483, 848, 860
BsnI GGCC 3 cut(s) 58, 79, 580
BsoBI CYCGRG 1 cut(s) 863
Bsp1286I GDGCHC 1 cut(s) 534
Bsp143I GATC 3 cut(s) 224, 457, 507
BspACI CCGC 3 cut(s) 720, 730, 831
BspANI GGCC 3 cut(s) 58, 79, 580
BspCNI CTCAG 1 cut(s) 151
BspFNI CGCG 1 cut(s) 772
BspLI GGNNCC 7 cut(s) 135, 241, 323, 728, 836, 837, 848
BspPI GGATC 2 cut(s) 219, 452
BspT107I GGYRCC 3 cut(s) 239, 321, 726
BspTI CTTAAG 1 cut(s) 574
BsrFI RCCGGY 1 cut(s) 695
BsrI ACTGG 3 cut(s) 80, 265, 462
BssAI RCCGGY 1 cut(s) 695
BssECI CCNNGG 2 cut(s) 317, 501
BssMI GATC 3 cut(s) 224, 457, 507
BssNAI GTATAC 2 cut(s) 375, 681
BssT1I CCWWGG 1 cut(s) 501
Bst1107I GTATAC 2 cut(s) 375, 681
Bst2UI CCWGG 2 cut(s) 69, 318
Bst4CI ACNGT 1 cut(s) 305
BstAFI CTTAAG 1 cut(s) 574
BstAPI GCANNNNNTGC 1 cut(s) 47
BstDEI CTNAG 2 cut(s) 87, 138
BstF5I GGATG 4 cut(s) 313, 418, 529, 613
BstFNI CGCG 1 cut(s) 772
BstKTI GATC 3 cut(s) 227, 460, 510
BstMBI GATC 3 cut(s) 224, 457, 507
BstMWI GCNNNNNNNGC 3 cut(s) 47, 64, 586
BstNI CCWGG 2 cut(s) 69, 318
BstSCI CCNGG 3 cut(s) 67, 316, 732
BstSLI GKGCMC 1 cut(s) 534
BstUI CGCG 1 cut(s) 772
BstX2I RGATCY 1 cut(s) 224
BstYI RGATCY 1 cut(s) 224
BstZ17I GTATAC 2 cut(s) 375, 681
BsuRI GGCC 3 cut(s) 58, 79, 580
BtgZI GCGATG 1 cut(s) 48
BtsCI GGATG 4 cut(s) 313, 418, 529, 613
BtsIMutI CAGTG 1 cut(s) 310
Cfr10I RCCGGY 1 cut(s) 695
Cfr13I GGNCC 5 cut(s) 134, 756, 786, 835, 847
CpoI CGGWCCG 1 cut(s) 756
Csp6I GTAC 2 cut(s) 322, 781
CspCI CAANNNNNGTGG 2 cut(s) 679, 714
CspI CGGWCCG 1 cut(s) 756
CviAII CATG 2 cut(s) 326, 790
CviQI GTAC 2 cut(s) 322, 781
DdeI CTNAG 2 cut(s) 87, 138
DpnI GATC 3 cut(s) 226, 459, 509
DpnII GATC 3 cut(s) 224, 457, 507
EaeI YGGCCR 2 cut(s) 56, 77
Eco130I CCWWGG 1 cut(s) 501
Eco32I GATATC 1 cut(s) 659
Eco47I GGWCC 5 cut(s) 134, 756, 786, 835, 847
Eco57I CTGAAG 1 cut(s) 648
Eco88I CYCGRG 1 cut(s) 863
EcoO109I RGGNCCY 2 cut(s) 134, 835
EcoRII CCWGG 2 cut(s) 67, 316
EcoRV GATATC 1 cut(s) 659
EcoT14I CCWWGG 1 cut(s) 501
EcoT22I ATGCAT 1 cut(s) 166
ErhI CCWWGG 1 cut(s) 501
FaeI CATG 2 cut(s) 329, 793
FalI AAGNNNNNCTT 2 cut(s) 431, 463
FaqI GGGAC 5 cut(s) 20, 367, 483, 848, 860
FatI CATG 2 cut(s) 325, 789
FauI CCCGC 2 cut(s) 727, 824
FblI GTMKAC 2 cut(s) 374, 680
Fnu4HI GCNGC 1 cut(s) 730
FokI GGATG 4 cut(s) 300, 425, 536, 620
Fsp4HI GCNGC 1 cut(s) 730
FspBI CTAG 1 cut(s) 549
GluI GCNGC 1 cut(s) 730
HaeIII GGCC 3 cut(s) 58, 79, 580
HapII CCGG 5 cut(s) 228, 696, 734, 754, 759
Hin1II CATG 2 cut(s) 329, 793
HindIII AAGCTT 2 cut(s) 148, 251
HinfI GANTC 3 cut(s) 117, 276, 496
HpaII CCGG 5 cut(s) 228, 696, 734, 754, 759
Hpy166II GTNNAC 4 cut(s) 337, 375, 394, 681
Hpy188I TCNGA 1 cut(s) 401
Hpy8I GTNNAC 4 cut(s) 337, 375, 394, 681
HpyAV CCTTC 1 cut(s) 760
HpyCH4III ACNGT 1 cut(s) 305
HpyCH4IV ACGT 1 cut(s) 566
HpyCH4V TGCA 5 cut(s) 41, 164, 382, 563, 674
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 64, 586
HpyF3I CTNAG 2 cut(s) 87, 138
HpySE526I ACGT 1 cut(s) 566
Hsp92II CATG 2 cut(s) 329, 793
KflI GGGWCCC 1 cut(s) 835
KpnI GGTACC 1 cut(s) 325
Kzo9I GATC 3 cut(s) 224, 457, 507
LweI GCATC 2 cut(s) 151, 598
MaeI CTAG 1 cut(s) 549
MaeII ACGT 1 cut(s) 566
MaeIII GTNAC 2 cut(s) 230, 263
MalI GATC 3 cut(s) 226, 459, 509
MboI GATC 3 cut(s) 224, 457, 507
MboII GAAGA 2 cut(s) 429, 641
MflI RGATCY 1 cut(s) 224
MhlI GDGCHC 1 cut(s) 534
MlsI TGGCCA 2 cut(s) 58, 79
MluCI AATT 5 cut(s) 24, 420, 469, 600, 685
MluNI TGGCCA 2 cut(s) 58, 79
MlyI GAGTC 2 cut(s) 285, 505
MnlI CCTC 8 cut(s) 29, 135, 147, 253, 395, 534, 754, 837
Mox20I TGGCCA 2 cut(s) 58, 79
Mph1103I ATGCAT 1 cut(s) 166
MscI TGGCCA 2 cut(s) 58, 79
MseI TTAA 4 cut(s) 108, 167, 249, 575
MslI CAYNNNNRTG 2 cut(s) 480, 865
Msp20I TGGCCA 2 cut(s) 58, 79
MspCI CTTAAG 1 cut(s) 574
MspI CCGG 5 cut(s) 228, 696, 734, 754, 759
MspR9I CCNGG 3 cut(s) 69, 318, 734
MvaI CCWGG 2 cut(s) 69, 318
MvnI CGCG 1 cut(s) 772
MwoI GCNNNNNNNGC 3 cut(s) 47, 64, 586
NciI CCSGG 1 cut(s) 734
NdeII GATC 3 cut(s) 224, 457, 507
NlaIII CATG 2 cut(s) 329, 793
NlaIV GGNNCC 7 cut(s) 135, 241, 323, 728, 836, 837, 848
NsiI ATGCAT 1 cut(s) 166
OliI CACNNNNGTG 1 cut(s) 865
PfeI GAWTC 1 cut(s) 117
PflMI CCANNNNNTGG 2 cut(s) 68, 795
PkrI GCNGC 1 cut(s) 731
PleI GAGTC 2 cut(s) 284, 504
PpsI GAGTC 2 cut(s) 284, 504
PpuMI RGGWCCY 2 cut(s) 134, 835
Psp1406I AACGTT 1 cut(s) 566
Psp5II RGGWCCY 2 cut(s) 134, 835
Psp6I CCWGG 2 cut(s) 67, 316
PspGI CCWGG 2 cut(s) 67, 316
PspN4I GGNNCC 7 cut(s) 135, 241, 323, 728, 836, 837, 848
PspPI GGNCC 5 cut(s) 134, 756, 786, 835, 847
PspPPI RGGWCCY 2 cut(s) 134, 835
PsuI RGATCY 1 cut(s) 224
RsaI GTAC 2 cut(s) 323, 782
RsaNI GTAC 2 cut(s) 322, 781
RseI CAYNNNNRTG 2 cut(s) 480, 865
Rsr2I CGGWCCG 1 cut(s) 756
RsrII CGGWCCG 1 cut(s) 756
SaqAI TTAA 4 cut(s) 108, 167, 249, 575
SatI GCNGC 1 cut(s) 730
Sau3AI GATC 3 cut(s) 224, 457, 507
Sau96I GGNCC 5 cut(s) 134, 756, 786, 835, 847
SchI GAGTC 2 cut(s) 285, 505
ScrFI CCNGG 3 cut(s) 69, 318, 734
SduI GDGCHC 1 cut(s) 534
SfaNI GCATC 2 cut(s) 151, 598
SinI GGWCC 5 cut(s) 134, 756, 786, 835, 847
SmiMI CAYNNNNRTG 2 cut(s) 480, 865
SmlI CTYRAG 1 cut(s) 574
SmoI CTYRAG 1 cut(s) 574
Sse9I AATT 5 cut(s) 24, 420, 469, 600, 685
SsiI CCGC 3 cut(s) 720, 730, 831
SspMI CTAG 1 cut(s) 549
StyD4I CCNGG 3 cut(s) 67, 316, 732
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 1 cut(s) 305
TaiI ACGT 1 cut(s) 569
TasI AATT 5 cut(s) 24, 420, 469, 600, 685
TatI WGTACW 1 cut(s) 780
TauI GCSGC 1 cut(s) 732
TfiI GAWTC 1 cut(s) 117
Tru1I TTAA 4 cut(s) 108, 167, 249, 575
Tru9I TTAA 4 cut(s) 108, 167, 249, 575
TscAI CASTG 1 cut(s) 310
TspDTI ATGAA 3 cut(s) 345, 452, 603
TspRI CASTG 1 cut(s) 310
Van91I CCANNNNNTGG 2 cut(s) 68, 795
Vha464I CTTAAG 1 cut(s) 574
VpaK11BI GGWCC 5 cut(s) 134, 756, 786, 835, 847
XapI RAATTY 1 cut(s) 469
XmiI GTMKAC 2 cut(s) 374, 680
XspI CTAG 1 cut(s) 549
Zsp2I ATGCAT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.