FvH4_6g17240

Pistil-specific extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0017690
Physical Location & Seq
Reverse (-)
11196729 .. 11199445
2717 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g17240.t1

Sequence Viewer

Length: 1434 bp
ATGGAGGGAAACAATCAAGAGAACAATGAGGAGGCAACAACAACTGATGAATATTTTCGCTCTCTTCCACCTGGTTATCGATTCAACCCTACTGATCAAGAGTTGATTCTGTATTATTTGAAGCCAAAGATCTTGAATCAACCGCTACCCCGTAATAGGATTCATGAAAGGAATATTTACCAATTCCATCCCCGAAAGCTTTCCGAGAAGTTCAAGAAAATTAGAGAAGATGGCTGGTATTTTTTCAGTACAAGGAAGCGAAAGTACCAGAATGGGAGACGGCCAAATCGTAAAGCAGACGATGGATATTGGAAGGCAACCGGAAGTGGTATAGATATCAAAGGTGAAAATGGTGAAGTAATTGGACACAAAAAAGTTCTGGATTATAAAAAGGGGGCGCATCCACTAAATATAAAGACGGATTGGAAAATGCTTGAATATAAACTTAAGGATGAGGATGAAGACAATCCTCTTCCAAAGAGGAAAAAAGGTGAAGGTAACATGAAGTTGGATCTTGTTTTGTGCAAAGTCTATTTGAATACCAGAGCTAATGGTAATACGAATAACAATGGTGCAATCACTACAGAAATTTCTGATCAAGAAATGGTTACAGATTCTGGTACATCTCATTACAGAGAAACAGTACTGAAGCAGGAAGCTGATCAAGTTCAACCTCTTGATTCATCACTGATGAGCAGCCTGCAACGACAGGTCCATAGTAATACTACCGGAGCATTCAACCATATGGATCACATATCGACTTCTTCCACAATACCCGAGACATGTTTTCATTCGTCAAATCCGATGAACAACGGCGTTATGGTTACAGATACTGTTACATCTCATCGTCGTCACAACAATTTAGTACCTAAGCAGGAAGCTGGTCAAGTTGCACCTCTTGATTCCTCACTGGTATACAGCCCGCAACCACAGGTACATAATAATACTACTGGAGGATTCAACCATTTGAATCACATATCCAATTCTTCTTCGTCAACTTCTATGAGCAACGGGGTTTATGACTCAACAAATTGTACACATTATGGTGAGCCTATGCACAATGGATCTTCTGGCTCCGTGTATCAGTGGGGATCCAGCTTCACTCAACTCCATCATCAAAACTATTACATGTACCCGGAGCAGATGCCATCATCTTCAACGTCGTATACTTCTGAACATCCCTTGCAATATTTGTCGACAGAGATTTGGACACAATTGCAGCCTGAAAACCATGAGATGTCTCGACTGGATGATTCAACTTTTGTTTCGTTACCTCCCCATGTCAGGGAGTACAATTCTATGCTTGTCAATATTAAGGACGAAGCTGAGATGATGCCCTTTAAGCACGAAGCTGATGATGATGATGATGATGATGGACTACAGTTTCTTAGCATTCCAGATGAAGTGCATGAAGATGATCCCAAAACAGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

478

Amino Acids

54.63

Weight (kDa)

5.68

Isoelectric Point (pI)

46.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 23 - 149 1.8e-37 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017690)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g17240
rosa_chinensis RchiOBHm_Chr3g0471331
rosa_laevigata RLG00000024186
rosa_multiflora Rmu_sc0010513.1_g000010 Rmu_sc0015755.1_g000003
rosa_roxburghii Rroxscaffold_6G00410050
rosa_rugosa Rorug03G0117200
rosa_samantha Rh3AG166800 Rh3BG192200 Rh3CG182500 Rh3DG183600
rosa_wichuraiana Rw3G015690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 387
AccI GTMKAC 3 cut(s) 915, 1166, 1196
AciI CCGC 2 cut(s) 143, 923
AclWI GGATC 6 cut(s) 519, 756, 1072, 1086, 1099, 1412
AcoI YGGCCR 1 cut(s) 281
AcsI RAATTY 1 cut(s) 588
AcuI CTGAAG 1 cut(s) 668
AfaI GTAC 9 cut(s) 250, 266, 622, 645, 867, 936, 1036, 1133, 1292
AfiI CCNNNNNNNGG 3 cut(s) 156, 1284, 1285
AflII CTTAAG 1 cut(s) 446
AflIII ACRYGT 2 cut(s) 782, 1128
AjnI CCWGG 1 cut(s) 70
AluBI AGCT 7 cut(s) 199, 548, 659, 881, 1098, 1325, 1352
AluI AGCT 7 cut(s) 199, 548, 659, 881, 1098, 1325, 1352
Alw26I GTCTC 3 cut(s) 271, 773, 1245
AlwI GGATC 6 cut(s) 519, 756, 1072, 1086, 1099, 1412
AlwNI CAGNNNCTG 2 cut(s) 617, 833
Ama87I CYCGRG 1 cut(s) 776
AoxI GGCC 1 cut(s) 281
ApeKI GCWGC 2 cut(s) 696, 1219
ApoI RAATTY 1 cut(s) 588
Asp700I GAANNNNTTC 2 cut(s) 54, 199
AspLEI GCGC 1 cut(s) 400
AspS9I GGNCC 1 cut(s) 712
AsuC2I CCSGG 1 cut(s) 1136
AsuHPI GGTGA 4 cut(s) 356, 365, 503, 1058
AvaI CYCGRG 1 cut(s) 776
AvaII GGWCC 1 cut(s) 712
BamHI GGATCC 1 cut(s) 1091
BarI GAAGNNNNNNTAC 2 cut(s) 248, 280
BbsI GAAGAC 1 cut(s) 468
BbvI GCAGC 2 cut(s) 708, 1231
BccI CCATC 6 cut(s) 195, 224, 296, 1119, 1156, 1367
BceAI ACGGC 2 cut(s) 296, 829
BciT130I CCWGG 1 cut(s) 72
BclI TGATCA 3 cut(s) 94, 595, 661
BcnI CCSGG 1 cut(s) 1136
BcoDI GTCTC 3 cut(s) 271, 773, 1245
BfmI CTRYAG 2 cut(s) 582, 1379
BfrI CTTAAG 1 cut(s) 446
BglII AGATCT 1 cut(s) 129
BisI GCNGC 2 cut(s) 697, 1220
BlsI GCNGC 2 cut(s) 698, 1221
BmcAI AGTACT 1 cut(s) 645
Bme1390I CCNGG 2 cut(s) 72, 1136
Bme18I GGWCC 1 cut(s) 712
BmeT110I CYCGRG 1 cut(s) 776
BmgT120I GGNCC 1 cut(s) 712
BmiI GGNNCC 2 cut(s) 1075, 1093
BmrFI CCNGG 2 cut(s) 72, 1136
BmsI GCATC 3 cut(s) 409, 1134, 1323
BpiI GAAGAC 1 cut(s) 468
BpmI CTGGAG 1 cut(s) 972
Bpu10I CCTNAGC 1 cut(s) 870
BpuMI CCSGG 1 cut(s) 1136
Bsa29I ATCGAT 1 cut(s) 79
BsaWI WCCGGW 2 cut(s) 320, 728
Bsc4I CCNNNNNNNGG 3 cut(s) 156, 1284, 1285
Bse1I ACTGG 3 cut(s) 915, 955, 1251
BseBI CCWGG 1 cut(s) 72
BseCI ATCGAT 1 cut(s) 79
BseGI GGATG 6 cut(s) 187, 400, 457, 463, 1177, 1255
BseLI CCNNNNNNNGG 3 cut(s) 156, 1284, 1285
BseMII CTCAG 1 cut(s) 1317
BseNI ACTGG 3 cut(s) 915, 955, 1251
BseRI GAGGAG 1 cut(s) 44
BseXI GCAGC 2 cut(s) 708, 1231
BshFI GGCC 1 cut(s) 283
BshVI ATCGAT 1 cut(s) 79
BsiHKCI CYCGRG 1 cut(s) 776
BsiSI CCGG 3 cut(s) 321, 729, 1136
BslI CCNNNNNNNGG 3 cut(s) 156, 1284, 1285
BsmAI GTCTC 3 cut(s) 271, 773, 1245
BsmBI CGTCTC 1 cut(s) 271
BsmI GAATGC 2 cut(s) 734, 1392
BsnI GGCC 1 cut(s) 283
BsoBI CYCGRG 1 cut(s) 776
Bsp1407I TGTACA 1 cut(s) 1034
Bsp143I GATC 9 cut(s) 94, 129, 511, 595, 661, 748, 1064, 1091, 1417
BspACI CCGC 2 cut(s) 143, 923
BspANI GGCC 1 cut(s) 283
BspCNI CTCAG 1 cut(s) 1318
BspDI ATCGAT 1 cut(s) 79
BspHI TCATGA 1 cut(s) 163
BspLI GGNNCC 2 cut(s) 1075, 1093
BspPI GGATC 6 cut(s) 519, 756, 1072, 1086, 1099, 1412
BspTI CTTAAG 1 cut(s) 446
BsrGI TGTACA 1 cut(s) 1034
BsrI ACTGG 3 cut(s) 915, 955, 1251
BssMI GATC 9 cut(s) 94, 129, 511, 595, 661, 748, 1064, 1091, 1417
BssNAI GTATAC 2 cut(s) 916, 1167
Bst1107I GTATAC 2 cut(s) 916, 1167
Bst2UI CCWGG 1 cut(s) 72
Bst4CI ACNGT 3 cut(s) 643, 835, 1383
Bst6I CTCTTC 2 cut(s) 69, 477
BstAFI CTTAAG 1 cut(s) 446
BstAUI TGTACA 1 cut(s) 1034
BstC8I GCNNGC 2 cut(s) 701, 923
BstDEI CTNAG 3 cut(s) 870, 1326, 1388
BstF5I GGATG 6 cut(s) 187, 400, 457, 463, 1177, 1255
BstHHI GCGC 1 cut(s) 400
BstKTI GATC 9 cut(s) 97, 132, 514, 598, 664, 751, 1067, 1094, 1420
BstMAI GTCTC 3 cut(s) 271, 773, 1245
BstMBI GATC 9 cut(s) 94, 129, 511, 595, 661, 748, 1064, 1091, 1417
BstMWI GCNNNNNNNGC 1 cut(s) 1342
BstNI CCWGG 1 cut(s) 72
BstNSI RCATGY 2 cut(s) 786, 1132
BstSCI CCNGG 2 cut(s) 70, 1134
BstSFI CTRYAG 2 cut(s) 582, 1379
BstV1I GCAGC 2 cut(s) 708, 1231
BstV2I GAAGAC 1 cut(s) 468
BstX2I RGATCY 4 cut(s) 129, 511, 1064, 1091
BstYI RGATCY 4 cut(s) 129, 511, 1064, 1091
BstZ17I GTATAC 2 cut(s) 916, 1167
Bsu15I ATCGAT 1 cut(s) 79
BsuRI GGCC 1 cut(s) 283
BsuTUI ATCGAT 1 cut(s) 79
BtsCI GGATG 6 cut(s) 187, 400, 457, 463, 1177, 1255
BtsIMutI CAGTG 3 cut(s) 686, 908, 1091
Cac8I GCNNGC 2 cut(s) 701, 923
CaiI CAGNNNCTG 2 cut(s) 617, 833
CciI TCATGA 1 cut(s) 163
CfoI GCGC 1 cut(s) 400
Cfr13I GGNCC 1 cut(s) 712
ClaI ATCGAT 1 cut(s) 79
CsiI ACCWGGT 1 cut(s) 70
Csp6I GTAC 9 cut(s) 249, 265, 621, 644, 866, 935, 1035, 1132, 1291
CviAII CATG 7 cut(s) 164, 502, 783, 1129, 1232, 1280, 1409
CviQI GTAC 9 cut(s) 249, 265, 621, 644, 866, 935, 1035, 1132, 1291
DdeI CTNAG 3 cut(s) 870, 1326, 1388
DpnI GATC 9 cut(s) 96, 131, 513, 597, 663, 750, 1066, 1093, 1419
DpnII GATC 9 cut(s) 94, 129, 511, 595, 661, 748, 1064, 1091, 1417
EaeI YGGCCR 1 cut(s) 281
Eam1104I CTCTTC 2 cut(s) 69, 477
EarI CTCTTC 2 cut(s) 69, 477
Eco32I GATATC 1 cut(s) 337
Eco47I GGWCC 1 cut(s) 712
Eco57I CTGAAG 1 cut(s) 668
Eco88I CYCGRG 1 cut(s) 776
EcoRII CCWGG 1 cut(s) 70
EcoRV GATATC 1 cut(s) 337
Esp3I CGTCTC 1 cut(s) 271
FaeI CATG 7 cut(s) 167, 505, 786, 1132, 1235, 1283, 1412
FatI CATG 7 cut(s) 163, 501, 782, 1128, 1231, 1279, 1408
FauI CCCGC 1 cut(s) 930
FauNDI CATATG 1 cut(s) 744
FbaI TGATCA 3 cut(s) 94, 595, 661
FblI GTMKAC 3 cut(s) 915, 1166, 1196
Fnu4HI GCNGC 2 cut(s) 697, 1220
FokI GGATG 6 cut(s) 174, 387, 464, 470, 1164, 1262
Fsp4HI GCNGC 2 cut(s) 697, 1220
GlaI GCGC 1 cut(s) 399
GluI GCNGC 2 cut(s) 697, 1220
GsuI CTGGAG 1 cut(s) 972
HaeIII GGCC 1 cut(s) 283
HapII CCGG 3 cut(s) 321, 729, 1136
HhaI GCGC 1 cut(s) 400
Hin1II CATG 7 cut(s) 167, 505, 786, 1132, 1235, 1283, 1412
Hin6I GCGC 1 cut(s) 398
HinP1I GCGC 1 cut(s) 398
HincII GTYRAC 2 cut(s) 996, 1197
HindII GTYRAC 2 cut(s) 996, 1197
HindIII AAGCTT 1 cut(s) 197
HpaII CCGG 3 cut(s) 321, 729, 1136
HphI GGTGA 4 cut(s) 356, 365, 503, 1058
Hpy166II GTNNAC 5 cut(s) 916, 996, 1037, 1167, 1197
Hpy188I TCNGA 4 cut(s) 205, 595, 804, 1174
Hpy8I GTNNAC 5 cut(s) 916, 996, 1037, 1167, 1197
Hpy99I CGWCG 2 cut(s) 852, 1165
HpyAV CCTTC 2 cut(s) 307, 488
HpyCH4III ACNGT 3 cut(s) 643, 835, 1383
HpyCH4IV ACGT 1 cut(s) 1160
HpyCH4V TGCA 8 cut(s) 525, 575, 703, 893, 1057, 1186, 1219, 1408
HpyF10VI GCNNNNNNNGC 1 cut(s) 1342
HpyF3I CTNAG 3 cut(s) 870, 1326, 1388
HpySE526I ACGT 1 cut(s) 1160
Hsp92II CATG 7 cut(s) 167, 505, 786, 1132, 1235, 1283, 1412
HspAI GCGC 1 cut(s) 398
Ksp22I TGATCA 3 cut(s) 94, 595, 661
Kzo9I GATC 9 cut(s) 94, 129, 511, 595, 661, 748, 1064, 1091, 1417
LmnI GCTCC 3 cut(s) 731, 1079, 1138
Lsp1109I GCAGC 2 cut(s) 708, 1231
LweI GCATC 3 cut(s) 409, 1134, 1323
MabI ACCWGGT 1 cut(s) 70
MaeII ACGT 1 cut(s) 1160
MaeIII GTNAC 6 cut(s) 497, 607, 823, 835, 851, 1269
MalI GATC 9 cut(s) 96, 131, 513, 597, 663, 750, 1066, 1093, 1419
MboI GATC 9 cut(s) 94, 129, 511, 595, 661, 748, 1064, 1091, 1417
MfeI CAATTG 1 cut(s) 1214
MflI RGATCY 4 cut(s) 129, 511, 1064, 1091
MluCI AATT 9 cut(s) 182, 219, 360, 588, 859, 982, 1030, 1214, 1294
MlyI GAGTC 1 cut(s) 1016
MmeI TCCRAC 1 cut(s) 489
MroXI GAANNNNTTC 2 cut(s) 54, 199
MseI TTAA 3 cut(s) 447, 1314, 1341
MslI CAYNNNNRTG 2 cut(s) 1044, 1413
MspCI CTTAAG 1 cut(s) 446
MspI CCGG 3 cut(s) 321, 729, 1136
MspR9I CCNGG 2 cut(s) 72, 1136
MunI CAATTG 1 cut(s) 1214
Mva1269I GAATGC 2 cut(s) 734, 1392
MvaI CCWGG 1 cut(s) 72
MwoI GCNNNNNNNGC 1 cut(s) 1342
NciI CCSGG 1 cut(s) 1136
NdeI CATATG 1 cut(s) 744
NdeII GATC 9 cut(s) 94, 129, 511, 595, 661, 748, 1064, 1091, 1417
NlaIII CATG 7 cut(s) 167, 505, 786, 1132, 1235, 1283, 1412
NlaIV GGNNCC 2 cut(s) 1075, 1093
NmuCI GTSAC 1 cut(s) 851
NspI RCATGY 2 cut(s) 786, 1132
PagI TCATGA 1 cut(s) 163
PciI ACATGT 2 cut(s) 782, 1128
PcsI WCGNNNNNNNCGW 2 cut(s) 286, 800
PctI GAATGC 2 cut(s) 734, 1392
PdmI GAANNNNTTC 2 cut(s) 54, 199
PkrI GCNGC 2 cut(s) 698, 1221
PleI GAGTC 1 cut(s) 1016
PpsI GAGTC 1 cut(s) 1016
PscI ACATGT 2 cut(s) 782, 1128
PsiI TTATAA 1 cut(s) 387
Psp6I CCWGG 1 cut(s) 70
PspGI CCWGG 1 cut(s) 70
PspN4I GGNNCC 2 cut(s) 1075, 1093
PspPI GGNCC 1 cut(s) 712
PstNI CAGNNNCTG 2 cut(s) 617, 833
PsuI RGATCY 4 cut(s) 129, 511, 1064, 1091
RsaI GTAC 9 cut(s) 250, 266, 622, 645, 867, 936, 1036, 1133, 1292
RsaNI GTAC 9 cut(s) 249, 265, 621, 644, 866, 935, 1035, 1132, 1291
RseI CAYNNNNRTG 2 cut(s) 1044, 1413
SalI GTCGAC 1 cut(s) 1195
SaqAI TTAA 3 cut(s) 447, 1314, 1341
SatI GCNGC 2 cut(s) 697, 1220
Sau3AI GATC 9 cut(s) 94, 129, 511, 595, 661, 748, 1064, 1091, 1417
Sau96I GGNCC 1 cut(s) 712
ScaI AGTACT 1 cut(s) 645
SchI GAGTC 1 cut(s) 1016
ScrFI CCNGG 2 cut(s) 72, 1136
SexAI ACCWGGT 1 cut(s) 70
SfaNI GCATC 3 cut(s) 409, 1134, 1323
SfcI CTRYAG 2 cut(s) 582, 1379
SinI GGWCC 1 cut(s) 712
SmiMI CAYNNNNRTG 2 cut(s) 1044, 1413
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
Sse9I AATT 9 cut(s) 182, 219, 360, 588, 859, 982, 1030, 1214, 1294
SsiI CCGC 2 cut(s) 143, 923
SspI AATATT 4 cut(s) 53, 175, 1190, 1312
StyD4I CCNGG 2 cut(s) 70, 1134
TaaI ACNGT 3 cut(s) 643, 835, 1383
TaiI ACGT 1 cut(s) 1163
TaqI TCGA 4 cut(s) 79, 758, 1196, 1243
TasI AATT 9 cut(s) 182, 219, 360, 588, 859, 982, 1030, 1214, 1294
TatI WGTACW 4 cut(s) 248, 643, 1034, 1290
Tru1I TTAA 3 cut(s) 447, 1314, 1341
Tru9I TTAA 3 cut(s) 447, 1314, 1341
TscAI CASTG 3 cut(s) 693, 915, 1091
TseFI GTSAC 1 cut(s) 851
TseI GCWGC 2 cut(s) 696, 1219
Tsp45I GTSAC 1 cut(s) 851
TspGWI ACGGA 2 cut(s) 434, 1066
TspRI CASTG 3 cut(s) 693, 915, 1091
Vha464I CTTAAG 1 cut(s) 446
VpaK11BI GGWCC 1 cut(s) 712
XapI RAATTY 1 cut(s) 588
XceI RCATGY 2 cut(s) 786, 1132
XmiI GTMKAC 3 cut(s) 915, 1166, 1196
XmnI GAANNNNTTC 2 cut(s) 54, 199
ZrmI AGTACT 1 cut(s) 645
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.