Rh3DG183600

Pistil-specific extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0017690
Physical Location & Seq
Forward (+)
16275541 .. 16278282
2742 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG183600.1

Sequence Viewer

Length: 1677 bp
ATGGAGGGAAACAGTCAAGAGATGAGTGCCGATGAATATTTTCGCTCTCTTCCACCTGGTTATCGATTCAACCCTACTGATGGAGAGTTGATTCTAAATTATTTGAACCGAAAGATCTTGAATCAGCCACTTCCCCGTAACAGGATCCATGACACAAATATTTACCAATACCATCCCCAAGAACTTTCAAAGATGTTCGACATAATTAGAGAAGATGGCTGGTATTTTTTCAGTAAAAGGGACCGAAAGTATCTAAATGGGACTCGGCCAAATCGTGCAGCTCGTGGTGGACATTGGAAGGCAACTGGAAGTGAAGTAAATATCATTGATGAAAATGGTGAACTAATTGGACACAAAAAAGTTCTCGAATTTAAAAAAGGGAGACATAAACAAGAAATAAAGACGGAATGGAAAATGCATGAATATAGAATTAAGGATGAGAAGGAAGACATTCCTCGTCCAAAGAGGAAAAAAGGTGAAGGAAATATGCAGTTGGATACTGTTTTGTGCAAAATCTATTTAAATACCAGAGGCAATGGTAATACGGATGAGAAAGATGCAGTCACTACAGAAACTTCTGATCAAGGAATTATGGTTACAGATGGTGGTACATCTCATGACAATGATATATCAGTACTTAAGCAAGTTCAACCTCTTGATTCATTATTAATGATGAGCAGCATGCAACCACAGGTCCATAATAATACTACCGGAGGATTCAACAATGTGGATCGCATATCCAATTCTTCCACAATACCCGATTGTTTTTATTCGTCGACTTCGATGAATAACGGAATTATGGTTACAGACACTGTTACATCTCATGACAAAGATATATCAGTAATTGAGCACGGACCTGGTCCACTTCAACCTCTTCGTTCCTCACTGATGTGCAGCCTGCAACCACAGGTCCATACTACCGGAGGATTCAAGAATATGGATCAAATATCCAATTCTTCCACGGTACCCAACATTTGTTTTTATTCATCAAATTCGATGAACGGAATTATGGTTACAGATACTGTTACATTTCATGATAAAGATATATCGGTACTTAAGCAGGGAGCTGGTCAAGTTCAACCTCTTGGTTCCTCACTGATGTGCAGCCTGCAACCGCAGGTCCATAATAATACTACCGGAGGATTCAACAATATGTATCACATATCCAATTCTTCCACAATACCCGACAATTGTTTTTATTCGTCAACTCCAATGAGCAACGGAGTATGTGACTCTACACAGTGTACGCATCATGGTGGGTCTATGTCAAATGGAGCTTCTGGCTCCGGGTATCGGTGGGGATCCTTCACCCAGCTCCATCATCAAAACTATAAGAATTACAGCATGTCCTCGGAGCAGATGCCCTCATCTTCGACGTGGTATCCTTCTGAGCATCCCTTGCAATATCCAACAGAGATTTGGACACAATCGGAGCCTGAAAATCATGAGATGCCTCAAGTGGATGATTCAACTTCAGTATCGTTACCTGCCCGATACATAAAATATGAGTCTATGCTTGTCAACAAGGAAGAAGCTGATATCATGCCTGTTCAGCATGCCAATAAGGACGAAGCTGATGAAGATGGATTACAGTTTCTTAGCATTCCAGATGAAGTTCATAAAAATGATTCCAAATTGGCGTCATTTGATGACGAAATTAACAGTGGACTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

558

Amino Acids

62.78

Weight (kDa)

5.48

Isoelectric Point (pI)

45.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 18 - 144 1.5e-38 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017690)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g17240
rosa_chinensis RchiOBHm_Chr3g0471331
rosa_laevigata RLG00000024186
rosa_multiflora Rmu_sc0010513.1_g000010 Rmu_sc0015755.1_g000003
rosa_roxburghii Rroxscaffold_6G00410050
rosa_rugosa Rorug03G0117200
rosa_samantha Rh3AG166800 Rh3BG192200 Rh3CG182500 Rh3DG183600
rosa_wichuraiana Rw3G015690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1108, 1495
Acc65I GGTACC 1 cut(s) 964
AccB1I GGYRCC 1 cut(s) 964
AccI GTMKAC 1 cut(s) 776
AciI CCGC 1 cut(s) 1115
AclWI GGATC 6 cut(s) 139, 152, 738, 948, 1296, 1309
AcoI YGGCCR 1 cut(s) 266
AcsI RAATTY 2 cut(s) 368, 991
AcuI CTGAAG 1 cut(s) 1458
AcyI GRCGYC 1 cut(s) 1640
AfaI GTAC 5 cut(s) 610, 636, 966, 1053, 1246
AfiI CCNNNNNNNGG 3 cut(s) 80, 141, 1293
AflII CTTAAG 2 cut(s) 638, 1055
AjiI CACGTC 1 cut(s) 1377
AjnI CCWGG 2 cut(s) 55, 856
AleI CACNNNNGTG 2 cut(s) 889, 1099
AluBI AGCT 6 cut(s) 281, 1067, 1277, 1315, 1535, 1574
AluI AGCT 6 cut(s) 281, 1067, 1277, 1315, 1535, 1574
Alw21I GWGCWC 1 cut(s) 852
Alw26I GTCTC 1 cut(s) 376
AlwI GGATC 6 cut(s) 139, 152, 738, 948, 1296, 1309
AlwNI CAGNNNCTG 2 cut(s) 812, 1022
AoxI GGCC 1 cut(s) 266
ApeKI GCWGC 4 cut(s) 278, 678, 894, 1104
ApoI RAATTY 2 cut(s) 368, 991
AseI ATTAAT 1 cut(s) 668
Asp700I GAANNNNTTC 2 cut(s) 39, 450
Asp718I GGTACC 1 cut(s) 964
AspS9I GGNCC 6 cut(s) 241, 694, 854, 860, 910, 1120
AsuC2I CCSGG 1 cut(s) 1288
AsuHPI GGTGA 3 cut(s) 350, 488, 1300
AvaII GGWCC 6 cut(s) 241, 694, 854, 860, 910, 1120
BaeI ACNNNNGTAYC 2 cut(s) 1461, 1494
BamHI GGATCC 2 cut(s) 144, 1301
BanI GGYRCC 1 cut(s) 964
BarI GAAGNNNNNNTAC 2 cut(s) 1572, 1604
BauI CACGAG 1 cut(s) 282
BbsI GAAGAC 1 cut(s) 453
Bbv12I GWGCWC 1 cut(s) 852
BbvI GCAGC 4 cut(s) 290, 690, 906, 1116
BccI CCATC 6 cut(s) 74, 180, 209, 596, 1326, 1577
BciT130I CCWGG 2 cut(s) 57, 858
BciVI GTATCC 2 cut(s) 490, 1392
BclI TGATCA 1 cut(s) 580
BcnI CCSGG 1 cut(s) 1288
BcoDI GTCTC 1 cut(s) 376
BfmI CTRYAG 1 cut(s) 567
BfrI CTTAAG 2 cut(s) 638, 1055
BfuAI ACCTGC 2 cut(s) 1108, 1495
BfuI GTATCC 2 cut(s) 490, 1392
BglII AGATCT 1 cut(s) 114
BisI GCNGC 4 cut(s) 279, 679, 895, 1105
BlsI GCNGC 4 cut(s) 280, 680, 896, 1106
BmcAI AGTACT 1 cut(s) 636
Bme1390I CCNGG 3 cut(s) 57, 858, 1288
Bme18I GGWCC 6 cut(s) 241, 694, 854, 860, 910, 1120
BmgBI CACGTC 1 cut(s) 1377
BmgT120I GGNCC 6 cut(s) 241, 694, 854, 860, 910, 1120
BmiI GGNNCC 7 cut(s) 146, 242, 966, 1090, 1285, 1303, 1434
BmrFI CCNGG 3 cut(s) 57, 858, 1288
BmsI GCATC 5 cut(s) 547, 1258, 1350, 1402, 1440
BpiI GAAGAC 1 cut(s) 453
BpuEI CTTGAG 1 cut(s) 1440
BpuMI CCSGG 1 cut(s) 1288
Bsa29I ATCGAT 1 cut(s) 64
BsaHI GRCGYC 1 cut(s) 1640
BsaJI CCNNGG 2 cut(s) 960, 1350
BsaWI WCCGGW 3 cut(s) 710, 920, 1136
Bsc4I CCNNNNNNNGG 3 cut(s) 80, 141, 1293
Bse1I ACTGG 1 cut(s) 310
Bse3DI GCAATG 1 cut(s) 541
BseBI CCWGG 2 cut(s) 57, 858
BseCI ATCGAT 1 cut(s) 64
BseDI CCNNGG 2 cut(s) 960, 1350
BseGI GGATG 5 cut(s) 172, 442, 553, 1393, 1468
BseLI CCNNNNNNNGG 3 cut(s) 80, 141, 1293
BseMI GCAATG 1 cut(s) 541
BseMII CTCAG 1 cut(s) 1380
BseNI ACTGG 1 cut(s) 310
BseXI GCAGC 4 cut(s) 290, 690, 906, 1116
BseYI CCCAGC 1 cut(s) 1311
BsgI GTGCAG 3 cut(s) 297, 913, 1123
BshFI GGCC 1 cut(s) 268
BshNI GGYRCC 1 cut(s) 964
BshVI ATCGAT 1 cut(s) 64
BsiHKAI GWGCWC 1 cut(s) 852
BsiSI CCGG 4 cut(s) 711, 921, 1137, 1287
BslFI GGGAC 2 cut(s) 254, 274
BslI CCNNNNNNNGG 3 cut(s) 80, 141, 1293
BsmAI GTCTC 1 cut(s) 376
BsmFI GGGAC 2 cut(s) 254, 274
BsmI GAATGC 1 cut(s) 1602
BsnI GGCC 1 cut(s) 268
Bsp1286I GDGCHC 1 cut(s) 852
Bsp143I GATC 6 cut(s) 114, 144, 580, 730, 940, 1301
BspACI CCGC 1 cut(s) 1115
BspANI GGCC 1 cut(s) 268
BspCNI CTCAG 1 cut(s) 1381
BspDI ATCGAT 1 cut(s) 64
BspHI TCATGA 4 cut(s) 616, 823, 1033, 1444
BspLI GGNNCC 7 cut(s) 146, 242, 966, 1090, 1285, 1303, 1434
BspMI ACCTGC 2 cut(s) 1108, 1495
BspPI GGATC 6 cut(s) 139, 152, 738, 948, 1296, 1309
BspT107I GGYRCC 1 cut(s) 964
BspTI CTTAAG 2 cut(s) 638, 1055
BsrDI GCAATG 1 cut(s) 541
BsrI ACTGG 1 cut(s) 310
BssECI CCNNGG 2 cut(s) 960, 1350
BssMI GATC 6 cut(s) 114, 144, 580, 730, 940, 1301
BssNI GRCGYC 1 cut(s) 1640
BssSI CACGAG 1 cut(s) 282
Bst2BI CACGAG 1 cut(s) 282
Bst2UI CCWGG 2 cut(s) 57, 858
Bst4CI ACNGT 8 cut(s) 14, 502, 814, 964, 1024, 1242, 1593, 1664
Bst6I CTCTTC 2 cut(s) 54, 879
BstACI GRCGYC 1 cut(s) 1640
BstAFI CTTAAG 2 cut(s) 638, 1055
BstAPI GCANNNNNTGC 1 cut(s) 1399
BstC8I GCNNGC 4 cut(s) 683, 899, 1109, 1557
BstDEI CTNAG 2 cut(s) 1389, 1598
BstDSI CCRYGG 1 cut(s) 960
BstF5I GGATG 5 cut(s) 172, 442, 553, 1393, 1468
BstKTI GATC 6 cut(s) 117, 147, 583, 733, 943, 1304
BstMAI GTCTC 1 cut(s) 376
BstMBI GATC 6 cut(s) 114, 144, 580, 730, 940, 1301
BstMWI GCNNNNNNNGC 2 cut(s) 1399, 1552
BstNI CCWGG 2 cut(s) 57, 858
BstNSI RCATGY 3 cut(s) 685, 1348, 1559
BstSCI CCNGG 3 cut(s) 55, 856, 1286
BstSFI CTRYAG 1 cut(s) 567
BstV1I GCAGC 4 cut(s) 290, 690, 906, 1116
BstV2I GAAGAC 1 cut(s) 453
BstX2I RGATCY 3 cut(s) 114, 144, 1301
BstYI RGATCY 3 cut(s) 114, 144, 1301
Bsu15I ATCGAT 1 cut(s) 64
BsuI GTATCC 2 cut(s) 490, 1392
BsuRI GGCC 1 cut(s) 268
BsuTUI ATCGAT 1 cut(s) 64
BtgI CCRYGG 1 cut(s) 960
BtrI CACGTC 1 cut(s) 1377
BtsCI GGATG 5 cut(s) 172, 442, 553, 1393, 1468
BtsIMutI CAGTG 5 cut(s) 810, 884, 1094, 1247, 1669
BveI ACCTGC 2 cut(s) 1108, 1495
Cac8I GCNNGC 4 cut(s) 683, 899, 1109, 1557
CaiI CAGNNNCTG 2 cut(s) 812, 1022
CciI TCATGA 4 cut(s) 616, 823, 1033, 1444
Cfr13I GGNCC 6 cut(s) 241, 694, 854, 860, 910, 1120
ClaI ATCGAT 1 cut(s) 64
CseI GACGC 1 cut(s) 1629
CsiI ACCWGGT 2 cut(s) 55, 856
Csp6I GTAC 5 cut(s) 609, 635, 965, 1052, 1245
CviQI GTAC 5 cut(s) 609, 635, 965, 1052, 1245
DdeI CTNAG 2 cut(s) 1389, 1598
DpnI GATC 6 cut(s) 116, 146, 582, 732, 942, 1303
DpnII GATC 6 cut(s) 114, 144, 580, 730, 940, 1301
DraI TTTAAA 2 cut(s) 373, 522
EaeI YGGCCR 1 cut(s) 266
Eam1104I CTCTTC 2 cut(s) 54, 879
EarI CTCTTC 2 cut(s) 54, 879
Eco32I GATATC 1 cut(s) 1540
Eco47I GGWCC 6 cut(s) 241, 694, 854, 860, 910, 1120
Eco57I CTGAAG 1 cut(s) 1458
EcoRII CCWGG 2 cut(s) 55, 856
EcoRV GATATC 1 cut(s) 1540
EcoT22I ATGCAT 1 cut(s) 420
FaqI GGGAC 2 cut(s) 254, 274
FbaI TGATCA 1 cut(s) 580
FblI GTMKAC 1 cut(s) 776
Fnu4HI GCNGC 4 cut(s) 279, 679, 895, 1105
FokI GGATG 5 cut(s) 159, 449, 560, 1380, 1475
Fsp4HI GCNGC 4 cut(s) 279, 679, 895, 1105
GluI GCNGC 4 cut(s) 279, 679, 895, 1105
GsaI CCCAGC 1 cut(s) 1315
HaeIII GGCC 1 cut(s) 268
HapII CCGG 4 cut(s) 711, 921, 1137, 1287
HgaI GACGC 1 cut(s) 1629
Hin1I GRCGYC 1 cut(s) 1640
HincII GTYRAC 3 cut(s) 777, 1206, 1522
HindII GTYRAC 3 cut(s) 777, 1206, 1522
HpaII CCGG 4 cut(s) 711, 921, 1137, 1287
HphI GGTGA 3 cut(s) 350, 488, 1300
Hpy166II GTNNAC 8 cut(s) 290, 341, 777, 863, 1206, 1245, 1522, 1667
Hpy188I TCNGA 4 cut(s) 580, 1354, 1390, 1432
Hpy8I GTNNAC 8 cut(s) 290, 341, 777, 863, 1206, 1245, 1522, 1667
Hpy99I CGWCG 2 cut(s) 778, 1378
HpyAV CCTTC 5 cut(s) 292, 436, 473, 1315, 1395
HpyCH4III ACNGT 8 cut(s) 14, 502, 814, 964, 1024, 1242, 1593, 1664
HpyCH4IV ACGT 1 cut(s) 1376
HpyF10VI GCNNNNNNNGC 2 cut(s) 1399, 1552
HpyF3I CTNAG 2 cut(s) 1389, 1598
HpySE526I ACGT 1 cut(s) 1376
Hsp92I GRCGYC 1 cut(s) 1640
KpnI GGTACC 1 cut(s) 968
Ksp22I TGATCA 1 cut(s) 580
Kzo9I GATC 6 cut(s) 114, 144, 580, 730, 940, 1301
LmnI GCTCC 6 cut(s) 1064, 1274, 1289, 1320, 1354, 1432
Lsp1109I GCAGC 4 cut(s) 290, 690, 906, 1116
LweI GCATC 5 cut(s) 547, 1258, 1350, 1402, 1440
MabI ACCWGGT 2 cut(s) 55, 856
MaeII ACGT 1 cut(s) 1376
MaeIII GTNAC 9 cut(s) 137, 562, 595, 802, 814, 1012, 1024, 1229, 1482
MalI GATC 6 cut(s) 116, 146, 582, 732, 942, 1303
MboI GATC 6 cut(s) 114, 144, 580, 730, 940, 1301
MfeI CAATTG 1 cut(s) 1189
MflI RGATCY 3 cut(s) 114, 144, 1301
MhlI GDGCHC 1 cut(s) 852
MlyI GAGTC 3 cut(s) 256, 1226, 1517
MmeI TCCRAC 2 cut(s) 474, 1433
Mph1103I ATGCAT 1 cut(s) 420
MroXI GAANNNNTTC 2 cut(s) 39, 450
MseI TTAA 7 cut(s) 372, 432, 521, 639, 668, 1056, 1659
MslI CAYNNNNRTG 5 cut(s) 621, 889, 1099, 1254, 1623
MspCI CTTAAG 2 cut(s) 638, 1055
MspI CCGG 4 cut(s) 711, 921, 1137, 1287
MspR9I CCNGG 3 cut(s) 57, 858, 1288
MunI CAATTG 1 cut(s) 1189
Mva1269I GAATGC 1 cut(s) 1602
MvaI CCWGG 2 cut(s) 57, 858
MwoI GCNNNNNNNGC 2 cut(s) 1399, 1552
NciI CCSGG 1 cut(s) 1288
NdeII GATC 6 cut(s) 114, 144, 580, 730, 940, 1301
NlaIV GGNNCC 7 cut(s) 146, 242, 966, 1090, 1285, 1303, 1434
NmeAIII GCCGAG 1 cut(s) 244
NmuCI GTSAC 2 cut(s) 562, 1229
NsiI ATGCAT 1 cut(s) 420
NspI RCATGY 3 cut(s) 685, 1348, 1559
OliI CACNNNNGTG 2 cut(s) 889, 1099
PaeI GCATGC 2 cut(s) 685, 1559
PagI TCATGA 4 cut(s) 616, 823, 1033, 1444
PcsI WCGNNNNNNNCGW 3 cut(s) 271, 280, 779
PctI GAATGC 1 cut(s) 1602
PdmI GAANNNNTTC 2 cut(s) 39, 450
PfeI GAWTC 9 cut(s) 66, 91, 121, 659, 717, 927, 1143, 1466, 1628
PflFI GACNNNGTC 1 cut(s) 858
PkrI GCNGC 4 cut(s) 280, 680, 896, 1106
PleI GAGTC 3 cut(s) 256, 1226, 1516
PpsI GAGTC 3 cut(s) 256, 1226, 1516
PshBI ATTAAT 1 cut(s) 668
Psp6I CCWGG 2 cut(s) 55, 856
PspFI CCCAGC 1 cut(s) 1311
PspGI CCWGG 2 cut(s) 55, 856
PspN4I GGNNCC 7 cut(s) 146, 242, 966, 1090, 1285, 1303, 1434
PspPI GGNCC 6 cut(s) 241, 694, 854, 860, 910, 1120
PstNI CAGNNNCTG 2 cut(s) 812, 1022
PsuI RGATCY 3 cut(s) 114, 144, 1301
PsyI GACNNNGTC 1 cut(s) 858
RsaI GTAC 5 cut(s) 610, 636, 966, 1053, 1246
RsaNI GTAC 5 cut(s) 609, 635, 965, 1052, 1245
RseI CAYNNNNRTG 5 cut(s) 621, 889, 1099, 1254, 1623
SalI GTCGAC 1 cut(s) 775
SaqAI TTAA 7 cut(s) 372, 432, 521, 639, 668, 1056, 1659
SatI GCNGC 4 cut(s) 279, 679, 895, 1105
Sau3AI GATC 6 cut(s) 114, 144, 580, 730, 940, 1301
Sau96I GGNCC 6 cut(s) 241, 694, 854, 860, 910, 1120
ScaI AGTACT 1 cut(s) 636
SchI GAGTC 3 cut(s) 256, 1226, 1517
ScrFI CCNGG 3 cut(s) 57, 858, 1288
SduI GDGCHC 1 cut(s) 852
SexAI ACCWGGT 2 cut(s) 55, 856
SfaNI GCATC 5 cut(s) 547, 1258, 1350, 1402, 1440
SfcI CTRYAG 1 cut(s) 567
SinI GGWCC 6 cut(s) 241, 694, 854, 860, 910, 1120
SmiI ATTTAAAT 1 cut(s) 522
SmiMI CAYNNNNRTG 5 cut(s) 621, 889, 1099, 1254, 1623
SmlI CTYRAG 3 cut(s) 638, 1055, 1455
SmoI CTYRAG 3 cut(s) 638, 1055, 1455
SphI GCATGC 2 cut(s) 685, 1559
SsiI CCGC 1 cut(s) 1115
SspI AATATT 2 cut(s) 38, 160
StyD4I CCNGG 3 cut(s) 55, 856, 1286
SwaI ATTTAAAT 1 cut(s) 522
TaaI ACNGT 8 cut(s) 14, 502, 814, 964, 1024, 1242, 1593, 1664
TaiI ACGT 1 cut(s) 1379
TaqI TCGA 7 cut(s) 64, 198, 366, 776, 782, 995, 1373
TaqII GACCGA 1 cut(s) 258
TatI WGTACW 1 cut(s) 634
TfiI GAWTC 9 cut(s) 66, 91, 121, 659, 717, 927, 1143, 1466, 1628
Tru1I TTAA 7 cut(s) 372, 432, 521, 639, 668, 1056, 1659
Tru9I TTAA 7 cut(s) 372, 432, 521, 639, 668, 1056, 1659
TscAI CASTG 5 cut(s) 817, 891, 1101, 1247, 1669
TseFI GTSAC 2 cut(s) 562, 1229
TseI GCWGC 4 cut(s) 278, 678, 894, 1104
Tsp45I GTSAC 2 cut(s) 562, 1229
TspGWI ACGGA 6 cut(s) 419, 560, 807, 867, 1017, 1236
TspRI CASTG 5 cut(s) 817, 891, 1101, 1247, 1669
Tth111I GACNNNGTC 1 cut(s) 858
Vha464I CTTAAG 2 cut(s) 638, 1055
VpaK11BI GGWCC 6 cut(s) 241, 694, 854, 860, 910, 1120
VspI ATTAAT 1 cut(s) 668
XapI RAATTY 2 cut(s) 368, 991
XceI RCATGY 3 cut(s) 685, 1348, 1559
XcmI CCANNNNNNNNNTGG 1 cut(s) 1416
XmiI GTMKAC 1 cut(s) 776
XmnI GAANNNNTTC 2 cut(s) 39, 450
ZrmI AGTACT 1 cut(s) 636
Zsp2I ATGCAT 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.