Rmu_sc0010513.1_g000010

Pistil-specific extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010513.1
OG0017690
Physical Location & Seq
Forward (+)
40946 .. 42525
1580 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010513.1_g000010.1.cds

Sequence Viewer

Length: 1503 bp
atgttcgacataattagagaagatggctggtattttttcagtaaaagggaccgaaagtatctaaatgggactcggccaaatcgtgcagctcgtggtggacgttggaaggcaactggaagtgaagtaaatatcattgatgaaaatggtgaactaattggacacaaaaaagttctcgaatttaaaaaagggagacataaacaagatataaagacggaatggaaaatgcatgaatatagaattaaggatgagaaggaagacattcctcgtccaaaaaggaaaaaaggtgaaggaaatatgcagttggatattgttttgtgcaaaatctatttaaataccagaggcaatggtaatacggatgagaaagatgcagtcactacagaaacttctgatcaaggaattatggttgcacttacagatgctggtacatctcatgacaatgatatatcagtacttaagcaggaagttggtcaagttcaacctcttgattcattattaatgatgagcagcatgcaaccacaggtccataataatactaccggaggattcaacaatgtggatcgcatatccaattcttccacaatagccgattggttttattcgtcgacttcgatgaataacggaattatggttacagacactgttacatctcatgacgaagatatatcagtacttgagcacggacctggtcaacttcaacctattggttcctcactgatgtgcagcctgcaaccacaggtccatactaccggaggattcaagaatatggatcacatatccaattcttccacggtacccaacatttgtttttattcatcaaattcgatgaacggaattatggttacagatactgttacatttcatgataaagatatatcggtacttaagcagggagctggtcaagttcaaccacttggttcctcactgatgtgcagcctgcaaccgcaggtccataataatactaccggaggattcaacaatatgtatcacatatccaattcttccacaataaccgacaattgtttttattcgtcaactccaatgagcaacggagtatgtgactctacacagcgtacgcatcatggtgggtctatgacaaatggagcttctggctccgggtatcggaggggatccttcacccagctccatcatcaaaactataagaattacagcatgtcctcggagcagatgccctcatcttcgacgtggtatccttctgagcatcccttgcaatatccaacagagatttggacacaatcggagcctgaaaatcatgagatgcctcaagtggatgattcaacttcagtatcgttacctgcccgatacatagaatatgagtctatgcttgtcaacaaggaagaagctgatatcatgcctgttcagcatgccaataaggacgaagctgatgatgatggattacagtttcttagcattccagatgaagttcataaaaatgattccaaattggcgtcatttgatgacgaaattaacagtggacttgactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

500

Amino Acids

55.83

Weight (kDa)

5.25

Isoelectric Point (pI)

43.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017690)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g17240
rosa_chinensis RchiOBHm_Chr3g0471331
rosa_laevigata RLG00000024186
rosa_multiflora Rmu_sc0010513.1_g000010 Rmu_sc0015755.1_g000003
rosa_roxburghii Rroxscaffold_6G00410050
rosa_rugosa Rorug03G0117200
rosa_samantha Rh3AG166800 Rh3BG192200 Rh3CG182500 Rh3DG183600
rosa_wichuraiana Rw3G015690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 934, 1321
Acc65I GGTACC 1 cut(s) 790
AccB1I GGYRCC 1 cut(s) 790
AccI GTMKAC 1 cut(s) 602
AciI CCGC 1 cut(s) 941
AclWI GGATC 4 cut(s) 564, 774, 1122, 1135
AcoI YGGCCR 1 cut(s) 74
AcsI RAATTY 2 cut(s) 176, 817
AcuI CTGAAG 1 cut(s) 1284
AcyI GRCGYC 1 cut(s) 1466
AfaI GTAC 6 cut(s) 424, 450, 669, 792, 879, 1072
AfiI CCNNNNNNNGG 1 cut(s) 1119
AflII CTTAAG 2 cut(s) 452, 881
AgsI TTSAA 7 cut(s) 476, 547, 695, 757, 905, 973, 1296
AjiI CACGTC 1 cut(s) 1203
AjnI CCWGG 1 cut(s) 682
AleI CACNNNNGTG 2 cut(s) 715, 925
AluBI AGCT 6 cut(s) 89, 893, 1103, 1141, 1361, 1400
AluI AGCT 6 cut(s) 89, 893, 1103, 1141, 1361, 1400
Alw21I GWGCWC 1 cut(s) 678
Alw26I GTCTC 1 cut(s) 184
AlwI GGATC 4 cut(s) 564, 774, 1122, 1135
AlwNI CAGNNNCTG 3 cut(s) 419, 638, 848
AoxI GGCC 1 cut(s) 74
ApeKI GCWGC 4 cut(s) 86, 504, 720, 930
ApoI RAATTY 2 cut(s) 176, 817
AseI ATTAAT 1 cut(s) 494
Asp700I GAANNNNTTC 1 cut(s) 258
Asp718I GGTACC 1 cut(s) 790
AspS9I GGNCC 5 cut(s) 49, 520, 680, 736, 946
AsuC2I CCSGG 1 cut(s) 1114
AsuHPI GGTGA 3 cut(s) 158, 296, 1126
AvaII GGWCC 5 cut(s) 49, 520, 680, 736, 946
BaeI ACNNNNGTAYC 2 cut(s) 1287, 1320
BamHI GGATCC 1 cut(s) 1127
BanI GGYRCC 1 cut(s) 790
BauI CACGAG 1 cut(s) 90
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 678
BbvI GCAGC 4 cut(s) 98, 516, 732, 942
BccI CCATC 3 cut(s) 17, 1152, 1403
BciT130I CCWGG 1 cut(s) 684
BciVI GTATCC 1 cut(s) 1218
BclI TGATCA 1 cut(s) 388
BcnI CCSGG 1 cut(s) 1114
BcoDI GTCTC 1 cut(s) 184
BfmI CTRYAG 1 cut(s) 375
BfrI CTTAAG 2 cut(s) 452, 881
BfuAI ACCTGC 2 cut(s) 934, 1321
BfuI GTATCC 1 cut(s) 1218
BisI GCNGC 4 cut(s) 87, 505, 721, 931
BlsI GCNGC 4 cut(s) 88, 506, 722, 932
BmcAI AGTACT 2 cut(s) 450, 669
Bme1390I CCNGG 2 cut(s) 684, 1114
Bme18I GGWCC 5 cut(s) 49, 520, 680, 736, 946
BmgBI CACGTC 1 cut(s) 1203
BmgT120I GGNCC 5 cut(s) 49, 520, 680, 736, 946
BmiI GGNNCC 7 cut(s) 50, 706, 792, 916, 1111, 1129, 1260
BmrFI CCNGG 2 cut(s) 684, 1114
BmsI GCATC 6 cut(s) 355, 406, 1084, 1176, 1228, 1266
BpiI GAAGAC 1 cut(s) 261
BpuEI CTTGAG 2 cut(s) 692, 1266
BpuMI CCSGG 1 cut(s) 1114
BsaHI GRCGYC 1 cut(s) 1466
BsaJI CCNNGG 2 cut(s) 786, 1176
BsaWI WCCGGW 3 cut(s) 536, 746, 962
Bsc4I CCNNNNNNNGG 1 cut(s) 1119
Bse1I ACTGG 1 cut(s) 118
Bse3DI GCAATG 1 cut(s) 349
BseBI CCWGG 1 cut(s) 684
BseDI CCNNGG 2 cut(s) 786, 1176
BseGI GGATG 4 cut(s) 250, 361, 1219, 1294
BseLI CCNNNNNNNGG 1 cut(s) 1119
BseMI GCAATG 1 cut(s) 349
BseMII CTCAG 1 cut(s) 1206
BseNI ACTGG 1 cut(s) 118
BseXI GCAGC 4 cut(s) 98, 516, 732, 942
BseYI CCCAGC 1 cut(s) 1137
BsgI GTGCAG 3 cut(s) 105, 739, 949
BshFI GGCC 1 cut(s) 76
BshNI GGYRCC 1 cut(s) 790
BsiHKAI GWGCWC 1 cut(s) 678
BsiSI CCGG 4 cut(s) 537, 747, 963, 1113
BsiWI CGTACG 1 cut(s) 1070
BslFI GGGAC 2 cut(s) 62, 82
BslI CCNNNNNNNGG 1 cut(s) 1119
BsmAI GTCTC 1 cut(s) 184
BsmFI GGGAC 2 cut(s) 62, 82
BsmI GAATGC 1 cut(s) 1428
BsnI GGCC 1 cut(s) 76
Bsp1286I GDGCHC 1 cut(s) 678
Bsp143I GATC 4 cut(s) 388, 556, 766, 1127
BspACI CCGC 1 cut(s) 941
BspANI GGCC 1 cut(s) 76
BspCNI CTCAG 1 cut(s) 1207
BspHI TCATGA 4 cut(s) 430, 649, 859, 1270
BspLI GGNNCC 7 cut(s) 50, 706, 792, 916, 1111, 1129, 1260
BspMI ACCTGC 2 cut(s) 934, 1321
BspPI GGATC 4 cut(s) 564, 774, 1122, 1135
BspT107I GGYRCC 1 cut(s) 790
BspTI CTTAAG 2 cut(s) 452, 881
BsrDI GCAATG 1 cut(s) 349
BsrI ACTGG 1 cut(s) 118
BssECI CCNNGG 2 cut(s) 786, 1176
BssMI GATC 4 cut(s) 388, 556, 766, 1127
BssNI GRCGYC 1 cut(s) 1466
BssSI CACGAG 1 cut(s) 90
Bst2BI CACGAG 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 684
Bst4CI ACNGT 5 cut(s) 640, 790, 850, 1419, 1490
BstACI GRCGYC 1 cut(s) 1466
BstAFI CTTAAG 2 cut(s) 452, 881
BstAPI GCANNNNNTGC 1 cut(s) 1225
BstC8I GCNNGC 4 cut(s) 509, 725, 935, 1383
BstDEI CTNAG 2 cut(s) 1215, 1424
BstDSI CCRYGG 1 cut(s) 786
BstF5I GGATG 4 cut(s) 250, 361, 1219, 1294
BstKTI GATC 4 cut(s) 391, 559, 769, 1130
BstMAI GTCTC 1 cut(s) 184
BstMBI GATC 4 cut(s) 388, 556, 766, 1127
BstMWI GCNNNNNNNGC 2 cut(s) 1225, 1378
BstNI CCWGG 1 cut(s) 684
BstNSI RCATGY 3 cut(s) 511, 1174, 1385
BstSCI CCNGG 2 cut(s) 682, 1112
BstSFI CTRYAG 1 cut(s) 375
BstV1I GCAGC 4 cut(s) 98, 516, 732, 942
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 1127
BstYI RGATCY 1 cut(s) 1127
BsuI GTATCC 1 cut(s) 1218
BsuRI GGCC 1 cut(s) 76
BtgI CCRYGG 1 cut(s) 786
BtrI CACGTC 1 cut(s) 1203
BtsCI GGATG 4 cut(s) 250, 361, 1219, 1294
BtsIMutI CAGTG 4 cut(s) 636, 710, 920, 1495
BveI ACCTGC 2 cut(s) 934, 1321
Cac8I GCNNGC 4 cut(s) 509, 725, 935, 1383
CaiI CAGNNNCTG 3 cut(s) 419, 638, 848
CciI TCATGA 4 cut(s) 430, 649, 859, 1270
Cfr13I GGNCC 5 cut(s) 49, 520, 680, 736, 946
CseI GACGC 1 cut(s) 1455
CsiI ACCWGGT 1 cut(s) 682
Csp6I GTAC 6 cut(s) 423, 449, 668, 791, 878, 1071
CviQI GTAC 6 cut(s) 423, 449, 668, 791, 878, 1071
DdeI CTNAG 2 cut(s) 1215, 1424
DpnI GATC 4 cut(s) 390, 558, 768, 1129
DpnII GATC 4 cut(s) 388, 556, 766, 1127
DraI TTTAAA 2 cut(s) 181, 330
EaeI YGGCCR 1 cut(s) 74
Eco32I GATATC 1 cut(s) 1366
Eco47I GGWCC 5 cut(s) 49, 520, 680, 736, 946
Eco57I CTGAAG 1 cut(s) 1284
EcoRII CCWGG 1 cut(s) 682
EcoRV GATATC 1 cut(s) 1366
EcoT22I ATGCAT 1 cut(s) 228
FaqI GGGAC 2 cut(s) 62, 82
FbaI TGATCA 1 cut(s) 388
FblI GTMKAC 1 cut(s) 602
Fnu4HI GCNGC 4 cut(s) 87, 505, 721, 931
FokI GGATG 4 cut(s) 257, 368, 1206, 1301
Fsp4HI GCNGC 4 cut(s) 87, 505, 721, 931
GluI GCNGC 4 cut(s) 87, 505, 721, 931
GsaI CCCAGC 1 cut(s) 1141
HaeIII GGCC 1 cut(s) 76
HapII CCGG 4 cut(s) 537, 747, 963, 1113
HgaI GACGC 1 cut(s) 1455
Hin1I GRCGYC 1 cut(s) 1466
HincII GTYRAC 4 cut(s) 603, 689, 1032, 1348
HindII GTYRAC 4 cut(s) 603, 689, 1032, 1348
HinfI GANTC 9 cut(s) 70, 485, 543, 753, 969, 1058, 1292, 1334, 1454
HpaII CCGG 4 cut(s) 537, 747, 963, 1113
HphI GGTGA 3 cut(s) 158, 296, 1126
Hpy166II GTNNAC 7 cut(s) 98, 149, 603, 689, 1032, 1348, 1493
Hpy188I TCNGA 5 cut(s) 388, 1122, 1180, 1216, 1258
Hpy188III TCNNGA 8 cut(s) 173, 431, 482, 650, 757, 860, 1271, 1433
Hpy8I GTNNAC 7 cut(s) 98, 149, 603, 689, 1032, 1348, 1493
Hpy99I CGWCG 2 cut(s) 604, 1204
HpyAV CCTTC 5 cut(s) 100, 244, 281, 1141, 1221
HpyCH4III ACNGT 5 cut(s) 640, 790, 850, 1419, 1490
HpyCH4IV ACGT 2 cut(s) 100, 1202
HpyF10VI GCNNNNNNNGC 2 cut(s) 1225, 1378
HpyF3I CTNAG 2 cut(s) 1215, 1424
HpySE526I ACGT 2 cut(s) 100, 1202
Hsp92I GRCGYC 1 cut(s) 1466
KpnI GGTACC 1 cut(s) 794
Ksp22I TGATCA 1 cut(s) 388
Kzo9I GATC 4 cut(s) 388, 556, 766, 1127
LmnI GCTCC 6 cut(s) 890, 1100, 1115, 1146, 1180, 1258
Lsp1109I GCAGC 4 cut(s) 98, 516, 732, 942
LweI GCATC 6 cut(s) 355, 406, 1084, 1176, 1228, 1266
MabI ACCWGGT 1 cut(s) 682
MaeII ACGT 2 cut(s) 100, 1202
MaeIII GTNAC 7 cut(s) 370, 628, 640, 838, 850, 1055, 1308
MalI GATC 4 cut(s) 390, 558, 768, 1129
MboI GATC 4 cut(s) 388, 556, 766, 1127
MboII GAAGA 8 cut(s) 32, 266, 564, 668, 774, 990, 1188, 1367
MfeI CAATTG 1 cut(s) 1015
MflI RGATCY 1 cut(s) 1127
MhlI GDGCHC 1 cut(s) 678
MlyI GAGTC 3 cut(s) 64, 1052, 1343
MmeI TCCRAC 3 cut(s) 83, 282, 1259
Mph1103I ATGCAT 1 cut(s) 228
MroXI GAANNNNTTC 1 cut(s) 258
MseI TTAA 7 cut(s) 180, 240, 329, 453, 494, 882, 1485
MslI CAYNNNNRTG 5 cut(s) 435, 715, 925, 1080, 1449
MspCI CTTAAG 2 cut(s) 452, 881
MspI CCGG 4 cut(s) 537, 747, 963, 1113
MspR9I CCNGG 2 cut(s) 684, 1114
MunI CAATTG 1 cut(s) 1015
Mva1269I GAATGC 1 cut(s) 1428
MvaI CCWGG 1 cut(s) 684
MwoI GCNNNNNNNGC 2 cut(s) 1225, 1378
NciI CCSGG 1 cut(s) 1114
NdeII GATC 4 cut(s) 388, 556, 766, 1127
NlaIV GGNNCC 7 cut(s) 50, 706, 792, 916, 1111, 1129, 1260
NmeAIII GCCGAG 1 cut(s) 52
NmuCI GTSAC 2 cut(s) 370, 1055
NsiI ATGCAT 1 cut(s) 228
NspI RCATGY 3 cut(s) 511, 1174, 1385
OliI CACNNNNGTG 2 cut(s) 715, 925
PaeI GCATGC 2 cut(s) 511, 1385
PagI TCATGA 4 cut(s) 430, 649, 859, 1270
PcsI WCGNNNNNNNCGW 4 cut(s) 79, 88, 97, 605
PctI GAATGC 1 cut(s) 1428
PdmI GAANNNNTTC 1 cut(s) 258
PfeI GAWTC 6 cut(s) 485, 543, 753, 969, 1292, 1454
Pfl23II CGTACG 1 cut(s) 1070
PflFI GACNNNGTC 1 cut(s) 684
PkrI GCNGC 4 cut(s) 88, 506, 722, 932
PleI GAGTC 3 cut(s) 64, 1052, 1342
PpsI GAGTC 3 cut(s) 64, 1052, 1342
PshBI ATTAAT 1 cut(s) 494
Psp6I CCWGG 1 cut(s) 682
PspFI CCCAGC 1 cut(s) 1137
PspGI CCWGG 1 cut(s) 682
PspLI CGTACG 1 cut(s) 1070
PspN4I GGNNCC 7 cut(s) 50, 706, 792, 916, 1111, 1129, 1260
PspPI GGNCC 5 cut(s) 49, 520, 680, 736, 946
PstNI CAGNNNCTG 3 cut(s) 419, 638, 848
PsuI RGATCY 1 cut(s) 1127
PsyI GACNNNGTC 1 cut(s) 684
RsaI GTAC 6 cut(s) 424, 450, 669, 792, 879, 1072
RsaNI GTAC 6 cut(s) 423, 449, 668, 791, 878, 1071
RseI CAYNNNNRTG 5 cut(s) 435, 715, 925, 1080, 1449
SalI GTCGAC 1 cut(s) 601
SaqAI TTAA 7 cut(s) 180, 240, 329, 453, 494, 882, 1485
SatI GCNGC 4 cut(s) 87, 505, 721, 931
Sau3AI GATC 4 cut(s) 388, 556, 766, 1127
Sau96I GGNCC 5 cut(s) 49, 520, 680, 736, 946
ScaI AGTACT 2 cut(s) 450, 669
SchI GAGTC 3 cut(s) 64, 1052, 1343
ScrFI CCNGG 2 cut(s) 684, 1114
SduI GDGCHC 1 cut(s) 678
SexAI ACCWGGT 1 cut(s) 682
SfaNI GCATC 6 cut(s) 355, 406, 1084, 1176, 1228, 1266
SfcI CTRYAG 1 cut(s) 375
SinI GGWCC 5 cut(s) 49, 520, 680, 736, 946
SmiI ATTTAAAT 1 cut(s) 330
SmiMI CAYNNNNRTG 5 cut(s) 435, 715, 925, 1080, 1449
SmlI CTYRAG 4 cut(s) 452, 671, 881, 1281
SmoI CTYRAG 4 cut(s) 452, 671, 881, 1281
SphI GCATGC 2 cut(s) 511, 1385
SsiI CCGC 1 cut(s) 941
StyD4I CCNGG 2 cut(s) 682, 1112
SwaI ATTTAAAT 1 cut(s) 330
TaaI ACNGT 5 cut(s) 640, 790, 850, 1419, 1490
TaiI ACGT 2 cut(s) 103, 1205
TaqI TCGA 6 cut(s) 6, 174, 602, 608, 821, 1199
TaqII GACCGA 1 cut(s) 66
TatI WGTACW 2 cut(s) 448, 667
TfiI GAWTC 6 cut(s) 485, 543, 753, 969, 1292, 1454
Tru1I TTAA 7 cut(s) 180, 240, 329, 453, 494, 882, 1485
Tru9I TTAA 7 cut(s) 180, 240, 329, 453, 494, 882, 1485
TscAI CASTG 4 cut(s) 643, 717, 927, 1495
TseFI GTSAC 2 cut(s) 370, 1055
TseI GCWGC 4 cut(s) 86, 504, 720, 930
Tsp45I GTSAC 2 cut(s) 370, 1055
TspDTI ATGAA 9 cut(s) 153, 243, 477, 626, 801, 839, 848, 1433, 1452
TspGWI ACGGA 6 cut(s) 227, 368, 633, 693, 843, 1062
TspRI CASTG 4 cut(s) 643, 717, 927, 1495
Tth111I GACNNNGTC 1 cut(s) 684
Vha464I CTTAAG 2 cut(s) 452, 881
VpaK11BI GGWCC 5 cut(s) 49, 520, 680, 736, 946
VspI ATTAAT 1 cut(s) 494
XapI RAATTY 2 cut(s) 176, 817
XceI RCATGY 3 cut(s) 511, 1174, 1385
XcmI CCANNNNNNNNNTGG 1 cut(s) 1242
XmiI GTMKAC 1 cut(s) 602
XmnI GAANNNNTTC 1 cut(s) 258
ZrmI AGTACT 2 cut(s) 450, 669
Zsp2I ATGCAT 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.