Rw3G015690

Pistil-specific extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0017690
Physical Location & Seq
Reverse (-)
16251758 .. 16254152
2395 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G015690.1

Sequence Viewer

Length: 1689 bp
ATGGAGGGAAACAGTCAAGAGATGAGTGCCGATGAATATTTTCGCTCTCTTCCACCTGGTTATCGATTCAACCCTACTGATGGAGAGTTGATTCTAAATTATTTGAACCGAAAGATCTTGAATCAGCCGCTTCCCCGTAACAGGATCCATGACATAAATATTTACCAATACCATCCCCAAGAACTTTCAAAGATGTTCGACATAATTAGAGAAGATGGCTGGTATTTTTTCAGTAAAAGGGACCGAAAGTATCTAAATGGGACTCGGCCAAATCGTGCAGCTCGTGGTGGACGTTGGAAGGCAACTGGAAGTGAAGTAAATATCATTGATGAAAATGGTGAACTAATTGGACACAAAAAAGTTCTCGAATTTAAAAAAGGGAGACATAAACAAGATATAAAGACGGAATGGAAAATGCATGAATATAGAATTAAGGATGAGAAGGAAGACATTCCTCGTCCAAAGAGGAAAAAAGGTGAAGGAAATATGCAGTTGGATACTGTTTTGTGCAAAATCTATTTAAATACCAGAGGCAATGGTAATACGGATGAGAAAGATGCAGTCACTACAGAAACTTCTGATCAAGGAATTATGGTTACAGATGCTGGTACATCTCATGACAATGATATATCAGTACTTAAGCAGGGAGTTGGTCAAGTTCAACCTCTTGATTCATTATTAATGATGAGCAGCATGCAACCACAGGTCCATAATAATACAACCGGAGGATTCAACAATGTGGATCGCATACCCAATTCTTCCACAATACCCGATTGTTTTTATTCGTCGACTTCGATGAATAACGGAATTATGGTTACAGACACTGTTACATCTCATGACAAAGATATATCAGTACTTGAGCACGGACCTGGTCAACTTCAACCTCTTGGTTCCTCACTGATGTGCAGCCTGCAACCACAGGTCCATACTATCGGAGGATTCAAGAATATGGATCACATATCCAATTCTTCCACGGTACCCAACATTTGTTTTTATTCATCAAATTCGATGAACGGAATTATGGTTACAGATACTGTTACATTTCATGATAAAGATATATCGGTACTTAAGCAGGGAGCTGGTCAAGTTCAACCTCTTGGTTCCTCACTGATGTGCAGCCTGCAACCGCAGGTCCATAATAATACTACCGGAGGATTCAACAATATGTATCACATATCCAATTCTTCCACAATACCCGAAAATTGTTTTTATTCGTCAACTCCAATGAGCAACGGAGTATGTGACTCTACACAGTGTACGCATCATGGTGGGTCTATGACAAATGGAGCTTCTGGCTCCGGTTATCGGTGGGGATCCTTCACCCATCTCCATCATCAAAACTATAAGAATTACAGCATGTCCTCGGGGCAGATGCCCTCATCTTCGACGTGGTATCCTTCTGAGCATCCCTTGCAATATCCAACAGAGATTTGGACACAATCGGAGCCTGAAAATCATGAGATGCCTCAAGTGGATGATTCAACTTCAGTATCGTTACCTGCCCGATACATAGAATATGAGTCTATGCTTGTCAACAAGGAAGAAGCTGATATCATGCCTGTTCAGCATGCCAATAAGGACGAAGCTGATGATGATGGATTACAGTTTCTTAGCATTCCAGATGAAGTTCATAAAAATGATTCCAAATTGGCGTCATTTGATGACGAAATTAACAGTGGACTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

562

Amino Acids

63.06

Weight (kDa)

5.46

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 18 - 144 7.7e-39 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017690)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g17240
rosa_chinensis RchiOBHm_Chr3g0471331
rosa_laevigata RLG00000024186
rosa_multiflora Rmu_sc0010513.1_g000010 Rmu_sc0015755.1_g000003
rosa_roxburghii Rroxscaffold_6G00410050
rosa_rugosa Rorug03G0117200
rosa_samantha Rh3AG166800 Rh3BG192200 Rh3CG182500 Rh3DG183600
rosa_wichuraiana Rw3G015690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1120, 1507
Acc65I GGTACC 1 cut(s) 976
AccB1I GGYRCC 1 cut(s) 976
AccI GTMKAC 1 cut(s) 788
AciI CCGC 2 cut(s) 128, 1127
AclWI GGATC 6 cut(s) 139, 152, 750, 960, 1308, 1321
AcoI YGGCCR 1 cut(s) 266
AcsI RAATTY 2 cut(s) 368, 1003
AcuI CTGAAG 1 cut(s) 1470
AcyI GRCGYC 1 cut(s) 1652
AfaI GTAC 6 cut(s) 610, 636, 855, 978, 1065, 1258
AfiI CCNNNNNNNGG 3 cut(s) 80, 141, 1305
AflII CTTAAG 2 cut(s) 638, 1067
AjiI CACGTC 1 cut(s) 1389
AjnI CCWGG 2 cut(s) 55, 868
AleI CACNNNNGTG 2 cut(s) 901, 1111
AluBI AGCT 5 cut(s) 281, 1079, 1289, 1547, 1586
AluI AGCT 5 cut(s) 281, 1079, 1289, 1547, 1586
Alw21I GWGCWC 1 cut(s) 864
Alw26I GTCTC 1 cut(s) 376
AlwI GGATC 6 cut(s) 139, 152, 750, 960, 1308, 1321
AlwNI CAGNNNCTG 3 cut(s) 605, 824, 1034
Ama87I CYCGRG 1 cut(s) 1363
AoxI GGCC 1 cut(s) 266
ApeKI GCWGC 4 cut(s) 278, 690, 906, 1116
ApoI RAATTY 2 cut(s) 368, 1003
AseI ATTAAT 1 cut(s) 680
Asp700I GAANNNNTTC 2 cut(s) 39, 450
Asp718I GGTACC 1 cut(s) 976
AspS9I GGNCC 5 cut(s) 241, 706, 866, 922, 1132
AsuHPI GGTGA 3 cut(s) 350, 488, 1312
AvaI CYCGRG 1 cut(s) 1363
AvaII GGWCC 5 cut(s) 241, 706, 866, 922, 1132
BaeI ACNNNNGTAYC 2 cut(s) 1473, 1506
BamHI GGATCC 2 cut(s) 144, 1313
BanI GGYRCC 1 cut(s) 976
BauI CACGAG 1 cut(s) 282
BbsI GAAGAC 1 cut(s) 453
Bbv12I GWGCWC 1 cut(s) 864
BbvI GCAGC 4 cut(s) 290, 702, 918, 1128
BccI CCATC 6 cut(s) 74, 180, 209, 1332, 1338, 1589
BciT130I CCWGG 2 cut(s) 57, 870
BciVI GTATCC 2 cut(s) 490, 1404
BclI TGATCA 1 cut(s) 580
BcoDI GTCTC 1 cut(s) 376
BfmI CTRYAG 1 cut(s) 567
BfrI CTTAAG 2 cut(s) 638, 1067
BfuAI ACCTGC 2 cut(s) 1120, 1507
BfuI GTATCC 2 cut(s) 490, 1404
BglII AGATCT 1 cut(s) 114
BisI GCNGC 5 cut(s) 128, 279, 691, 907, 1117
BlsI GCNGC 5 cut(s) 129, 280, 692, 908, 1118
BmcAI AGTACT 2 cut(s) 636, 855
Bme1390I CCNGG 2 cut(s) 57, 870
Bme18I GGWCC 5 cut(s) 241, 706, 866, 922, 1132
BmeT110I CYCGRG 1 cut(s) 1363
BmgBI CACGTC 1 cut(s) 1389
BmgT120I GGNCC 5 cut(s) 241, 706, 866, 922, 1132
BmiI GGNNCC 8 cut(s) 146, 242, 892, 978, 1102, 1297, 1315, 1446
BmrFI CCNGG 2 cut(s) 57, 870
BmsI GCATC 6 cut(s) 547, 592, 1270, 1362, 1414, 1452
BpiI GAAGAC 1 cut(s) 453
BpuEI CTTGAG 2 cut(s) 878, 1452
Bsa29I ATCGAT 1 cut(s) 64
BsaHI GRCGYC 1 cut(s) 1652
BsaJI CCNNGG 2 cut(s) 972, 1362
BsaWI WCCGGW 3 cut(s) 722, 1148, 1298
Bsc4I CCNNNNNNNGG 3 cut(s) 80, 141, 1305
Bse1I ACTGG 1 cut(s) 310
Bse3DI GCAATG 1 cut(s) 541
BseBI CCWGG 2 cut(s) 57, 870
BseCI ATCGAT 1 cut(s) 64
BseDI CCNNGG 2 cut(s) 972, 1362
BseGI GGATG 5 cut(s) 172, 442, 553, 1405, 1480
BseLI CCNNNNNNNGG 3 cut(s) 80, 141, 1305
BseMI GCAATG 1 cut(s) 541
BseMII CTCAG 1 cut(s) 1392
BseNI ACTGG 1 cut(s) 310
BseXI GCAGC 4 cut(s) 290, 702, 918, 1128
BsgI GTGCAG 3 cut(s) 297, 925, 1135
BshFI GGCC 1 cut(s) 268
BshNI GGYRCC 1 cut(s) 976
BshVI ATCGAT 1 cut(s) 64
BsiHKAI GWGCWC 1 cut(s) 864
BsiHKCI CYCGRG 1 cut(s) 1363
BsiSI CCGG 3 cut(s) 723, 1149, 1299
BslFI GGGAC 2 cut(s) 254, 274
BslI CCNNNNNNNGG 3 cut(s) 80, 141, 1305
BsmAI GTCTC 1 cut(s) 376
BsmFI GGGAC 2 cut(s) 254, 274
BsmI GAATGC 1 cut(s) 1614
BsnI GGCC 1 cut(s) 268
BsoBI CYCGRG 1 cut(s) 1363
Bsp1286I GDGCHC 1 cut(s) 864
Bsp143I GATC 6 cut(s) 114, 144, 580, 742, 952, 1313
BspACI CCGC 2 cut(s) 128, 1127
BspANI GGCC 1 cut(s) 268
BspCNI CTCAG 1 cut(s) 1393
BspDI ATCGAT 1 cut(s) 64
BspHI TCATGA 4 cut(s) 616, 835, 1045, 1456
BspLI GGNNCC 8 cut(s) 146, 242, 892, 978, 1102, 1297, 1315, 1446
BspMI ACCTGC 2 cut(s) 1120, 1507
BspPI GGATC 6 cut(s) 139, 152, 750, 960, 1308, 1321
BspT107I GGYRCC 1 cut(s) 976
BspTI CTTAAG 2 cut(s) 638, 1067
BsrDI GCAATG 1 cut(s) 541
BsrI ACTGG 1 cut(s) 310
BssECI CCNNGG 2 cut(s) 972, 1362
BssMI GATC 6 cut(s) 114, 144, 580, 742, 952, 1313
BssNI GRCGYC 1 cut(s) 1652
BssSI CACGAG 1 cut(s) 282
Bst2BI CACGAG 1 cut(s) 282
Bst2UI CCWGG 2 cut(s) 57, 870
Bst4CI ACNGT 8 cut(s) 14, 502, 826, 976, 1036, 1254, 1605, 1676
Bst6I CTCTTC 1 cut(s) 54
BstACI GRCGYC 1 cut(s) 1652
BstAFI CTTAAG 2 cut(s) 638, 1067
BstAPI GCANNNNNTGC 1 cut(s) 1411
BstC8I GCNNGC 4 cut(s) 695, 911, 1121, 1569
BstDEI CTNAG 2 cut(s) 1401, 1610
BstDSI CCRYGG 1 cut(s) 972
BstF5I GGATG 5 cut(s) 172, 442, 553, 1405, 1480
BstKTI GATC 6 cut(s) 117, 147, 583, 745, 955, 1316
BstMAI GTCTC 1 cut(s) 376
BstMBI GATC 6 cut(s) 114, 144, 580, 742, 952, 1313
BstMWI GCNNNNNNNGC 2 cut(s) 1411, 1564
BstNI CCWGG 2 cut(s) 57, 870
BstNSI RCATGY 3 cut(s) 697, 1360, 1571
BstSCI CCNGG 2 cut(s) 55, 868
BstSFI CTRYAG 1 cut(s) 567
BstV1I GCAGC 4 cut(s) 290, 702, 918, 1128
BstV2I GAAGAC 1 cut(s) 453
BstX2I RGATCY 3 cut(s) 114, 144, 1313
BstYI RGATCY 3 cut(s) 114, 144, 1313
Bsu15I ATCGAT 1 cut(s) 64
BsuI GTATCC 2 cut(s) 490, 1404
BsuRI GGCC 1 cut(s) 268
BsuTUI ATCGAT 1 cut(s) 64
BtgI CCRYGG 1 cut(s) 972
BtrI CACGTC 1 cut(s) 1389
BtsCI GGATG 5 cut(s) 172, 442, 553, 1405, 1480
BtsIMutI CAGTG 5 cut(s) 822, 896, 1106, 1259, 1681
BveI ACCTGC 2 cut(s) 1120, 1507
Cac8I GCNNGC 4 cut(s) 695, 911, 1121, 1569
CaiI CAGNNNCTG 3 cut(s) 605, 824, 1034
CciI TCATGA 4 cut(s) 616, 835, 1045, 1456
Cfr13I GGNCC 5 cut(s) 241, 706, 866, 922, 1132
ClaI ATCGAT 1 cut(s) 64
CseI GACGC 1 cut(s) 1641
CsiI ACCWGGT 2 cut(s) 55, 868
Csp6I GTAC 6 cut(s) 609, 635, 854, 977, 1064, 1257
CviQI GTAC 6 cut(s) 609, 635, 854, 977, 1064, 1257
DdeI CTNAG 2 cut(s) 1401, 1610
DpnI GATC 6 cut(s) 116, 146, 582, 744, 954, 1315
DpnII GATC 6 cut(s) 114, 144, 580, 742, 952, 1313
DraI TTTAAA 2 cut(s) 373, 522
EaeI YGGCCR 1 cut(s) 266
Eam1104I CTCTTC 1 cut(s) 54
EarI CTCTTC 1 cut(s) 54
Eco32I GATATC 1 cut(s) 1552
Eco47I GGWCC 5 cut(s) 241, 706, 866, 922, 1132
Eco57I CTGAAG 1 cut(s) 1470
Eco88I CYCGRG 1 cut(s) 1363
EcoRII CCWGG 2 cut(s) 55, 868
EcoRV GATATC 1 cut(s) 1552
EcoT22I ATGCAT 1 cut(s) 420
FaqI GGGAC 2 cut(s) 254, 274
FbaI TGATCA 1 cut(s) 580
FblI GTMKAC 1 cut(s) 788
Fnu4HI GCNGC 5 cut(s) 128, 279, 691, 907, 1117
FokI GGATG 5 cut(s) 159, 449, 560, 1392, 1487
Fsp4HI GCNGC 5 cut(s) 128, 279, 691, 907, 1117
GluI GCNGC 5 cut(s) 128, 279, 691, 907, 1117
HaeIII GGCC 1 cut(s) 268
HapII CCGG 3 cut(s) 723, 1149, 1299
HgaI GACGC 1 cut(s) 1641
Hin1I GRCGYC 1 cut(s) 1652
HincII GTYRAC 4 cut(s) 789, 875, 1218, 1534
HindII GTYRAC 4 cut(s) 789, 875, 1218, 1534
HpaII CCGG 3 cut(s) 723, 1149, 1299
HphI GGTGA 3 cut(s) 350, 488, 1312
Hpy166II GTNNAC 8 cut(s) 290, 341, 789, 875, 1218, 1257, 1534, 1679
Hpy188I TCNGA 4 cut(s) 580, 935, 1402, 1444
Hpy8I GTNNAC 8 cut(s) 290, 341, 789, 875, 1218, 1257, 1534, 1679
Hpy99I CGWCG 2 cut(s) 790, 1390
HpyAV CCTTC 5 cut(s) 292, 436, 473, 1327, 1407
HpyCH4III ACNGT 8 cut(s) 14, 502, 826, 976, 1036, 1254, 1605, 1676
HpyCH4IV ACGT 2 cut(s) 292, 1388
HpyF10VI GCNNNNNNNGC 2 cut(s) 1411, 1564
HpyF3I CTNAG 2 cut(s) 1401, 1610
HpySE526I ACGT 2 cut(s) 292, 1388
Hsp92I GRCGYC 1 cut(s) 1652
KpnI GGTACC 1 cut(s) 980
Ksp22I TGATCA 1 cut(s) 580
Kzo9I GATC 6 cut(s) 114, 144, 580, 742, 952, 1313
LmnI GCTCC 4 cut(s) 1076, 1286, 1301, 1444
Lsp1109I GCAGC 4 cut(s) 290, 702, 918, 1128
LweI GCATC 6 cut(s) 547, 592, 1270, 1362, 1414, 1452
MabI ACCWGGT 2 cut(s) 55, 868
MaeII ACGT 2 cut(s) 292, 1388
MaeIII GTNAC 9 cut(s) 137, 562, 595, 814, 826, 1024, 1036, 1241, 1494
MalI GATC 6 cut(s) 116, 146, 582, 744, 954, 1315
MboI GATC 6 cut(s) 114, 144, 580, 742, 952, 1313
MboII GAAGA 8 cut(s) 41, 224, 458, 750, 960, 1176, 1374, 1553
MflI RGATCY 3 cut(s) 114, 144, 1313
MhlI GDGCHC 1 cut(s) 864
MlyI GAGTC 3 cut(s) 256, 1238, 1529
MmeI TCCRAC 3 cut(s) 275, 474, 1445
Mph1103I ATGCAT 1 cut(s) 420
MroXI GAANNNNTTC 2 cut(s) 39, 450
MseI TTAA 7 cut(s) 372, 432, 521, 639, 680, 1068, 1671
MslI CAYNNNNRTG 5 cut(s) 621, 901, 1111, 1266, 1635
MspCI CTTAAG 2 cut(s) 638, 1067
MspI CCGG 3 cut(s) 723, 1149, 1299
MspR9I CCNGG 2 cut(s) 57, 870
Mva1269I GAATGC 1 cut(s) 1614
MvaI CCWGG 2 cut(s) 57, 870
MwoI GCNNNNNNNGC 2 cut(s) 1411, 1564
NdeII GATC 6 cut(s) 114, 144, 580, 742, 952, 1313
NlaIV GGNNCC 8 cut(s) 146, 242, 892, 978, 1102, 1297, 1315, 1446
NmeAIII GCCGAG 1 cut(s) 244
NmuCI GTSAC 2 cut(s) 562, 1241
NsiI ATGCAT 1 cut(s) 420
NspI RCATGY 3 cut(s) 697, 1360, 1571
OliI CACNNNNGTG 2 cut(s) 901, 1111
PaeI GCATGC 2 cut(s) 697, 1571
PagI TCATGA 4 cut(s) 616, 835, 1045, 1456
PcsI WCGNNNNNNNCGW 4 cut(s) 271, 280, 289, 791
PctI GAATGC 1 cut(s) 1614
PdmI GAANNNNTTC 2 cut(s) 39, 450
PfeI GAWTC 9 cut(s) 66, 91, 121, 671, 729, 939, 1155, 1478, 1640
PflFI GACNNNGTC 1 cut(s) 870
PkrI GCNGC 5 cut(s) 129, 280, 692, 908, 1118
PleI GAGTC 3 cut(s) 256, 1238, 1528
PpsI GAGTC 3 cut(s) 256, 1238, 1528
PshBI ATTAAT 1 cut(s) 680
Psp6I CCWGG 2 cut(s) 55, 868
PspGI CCWGG 2 cut(s) 55, 868
PspN4I GGNNCC 8 cut(s) 146, 242, 892, 978, 1102, 1297, 1315, 1446
PspPI GGNCC 5 cut(s) 241, 706, 866, 922, 1132
PstNI CAGNNNCTG 3 cut(s) 605, 824, 1034
PsuI RGATCY 3 cut(s) 114, 144, 1313
PsyI GACNNNGTC 1 cut(s) 870
RsaI GTAC 6 cut(s) 610, 636, 855, 978, 1065, 1258
RsaNI GTAC 6 cut(s) 609, 635, 854, 977, 1064, 1257
RseI CAYNNNNRTG 5 cut(s) 621, 901, 1111, 1266, 1635
SalI GTCGAC 1 cut(s) 787
SaqAI TTAA 7 cut(s) 372, 432, 521, 639, 680, 1068, 1671
SatI GCNGC 5 cut(s) 128, 279, 691, 907, 1117
Sau3AI GATC 6 cut(s) 114, 144, 580, 742, 952, 1313
Sau96I GGNCC 5 cut(s) 241, 706, 866, 922, 1132
ScaI AGTACT 2 cut(s) 636, 855
SchI GAGTC 3 cut(s) 256, 1238, 1529
ScrFI CCNGG 2 cut(s) 57, 870
SduI GDGCHC 1 cut(s) 864
SexAI ACCWGGT 2 cut(s) 55, 868
SfaNI GCATC 6 cut(s) 547, 592, 1270, 1362, 1414, 1452
SfcI CTRYAG 1 cut(s) 567
SinI GGWCC 5 cut(s) 241, 706, 866, 922, 1132
SmiI ATTTAAAT 1 cut(s) 522
SmiMI CAYNNNNRTG 5 cut(s) 621, 901, 1111, 1266, 1635
SmlI CTYRAG 4 cut(s) 638, 857, 1067, 1467
SmoI CTYRAG 4 cut(s) 638, 857, 1067, 1467
SphI GCATGC 2 cut(s) 697, 1571
SsiI CCGC 2 cut(s) 128, 1127
SspI AATATT 2 cut(s) 38, 160
StyD4I CCNGG 2 cut(s) 55, 868
SwaI ATTTAAAT 1 cut(s) 522
TaaI ACNGT 8 cut(s) 14, 502, 826, 976, 1036, 1254, 1605, 1676
TaiI ACGT 2 cut(s) 295, 1391
TaqI TCGA 7 cut(s) 64, 198, 366, 788, 794, 1007, 1385
TaqII GACCGA 1 cut(s) 258
TatI WGTACW 2 cut(s) 634, 853
TauI GCSGC 1 cut(s) 130
TfiI GAWTC 9 cut(s) 66, 91, 121, 671, 729, 939, 1155, 1478, 1640
Tru1I TTAA 7 cut(s) 372, 432, 521, 639, 680, 1068, 1671
Tru9I TTAA 7 cut(s) 372, 432, 521, 639, 680, 1068, 1671
TscAI CASTG 5 cut(s) 829, 903, 1113, 1259, 1681
TseFI GTSAC 2 cut(s) 562, 1241
TseI GCWGC 4 cut(s) 278, 690, 906, 1116
Tsp45I GTSAC 2 cut(s) 562, 1241
TspGWI ACGGA 6 cut(s) 419, 560, 819, 879, 1029, 1248
TspRI CASTG 5 cut(s) 829, 903, 1113, 1259, 1681
Tth111I GACNNNGTC 1 cut(s) 870
Vha464I CTTAAG 2 cut(s) 638, 1067
VpaK11BI GGWCC 5 cut(s) 241, 706, 866, 922, 1132
VspI ATTAAT 1 cut(s) 680
XapI RAATTY 2 cut(s) 368, 1003
XceI RCATGY 3 cut(s) 697, 1360, 1571
XcmI CCANNNNNNNNNTGG 1 cut(s) 1428
XmiI GTMKAC 1 cut(s) 788
XmnI GAANNNNTTC 2 cut(s) 39, 450
ZrmI AGTACT 2 cut(s) 636, 855
Zsp2I ATGCAT 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.