Rroxscaffold_6G00410050

Pistil-specific extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0017690
Physical Location & Seq
Forward (+)
32524225 .. 32526615
2391 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00410050.1

Sequence Viewer

Length: 1695 bp
ATGGAGGGAAACAGTCAAGAGATGAGTGCTGATGAATATTTTCGCTCTCTTCCACCTGGTTATCGATTCAACCCTACTGATGGAGAGTTGATTCTAAATTATTTGAACCGAAAGATCTTGAATCAGCCAGTTCCCCGTAACAGGATCCATGACATAAATATTTACCAATACCATCCCCAAGAGCTTTCAAAGATGTTCGACATAATTAGAGAAGATGGCTGGTATTTTTTCAGTAAAAGGGACCGAAAGTATCTAAATGGGACTCGGCCAAATCGTGCAGCTCGTGGTGGACGTTGGAAGGCAACTGGAAGTGAAGTAAATATCATTGATGAAAATGGTGAACTAATTGGACACAAAAAAGTTCTCGAATTTAAAAAAGGGAGACATAAACAAGATATAAAGACAGAATGGAAAATGCATGAATATAGAATTAAGGATGAGAAGGAAGACATTCCTCGTCCAAAGAGGAAAAAAGGTGAAGGAAATATGAAGTTGGATACTGTTTTGTGCAAAATCTATTTAAATACCAGAGGCAATGGTAATACAGATGAGAAAGATGCAGTCACTACAGAAACTTCTGATCAAGGAATTATGGTTACAGATGCTGGTACATCTCATGACAATGATATATCAGTACTTAAGCAGCAGGGAGTTGGTCAAGTTCAACCTCTTGATTCATTATTAAGGATGAGCAGCATGCAACCACAGGTCCATAATAATACTACCGGAGGATTCAACAATGTGGATCGCATATCCAATTCTTCCACAATACCCGATTGTTTTTATTCGTCGACTTCGATGAGTAACGGAATTATGGTTACAGACAGTACTGTTACATCTCATGACAAAGATATATCAGTACTTGAGCACGGACCTGGTCAACTTCAACCTCTTGGTTCCTCACTAATGTGCAGCCTGCAACCACAGGTCCATACTACCGGAGGATTCAAGAATATGGATTACATATCCAATTCTTCCACAGTACCCAACATTTGTTTTTATTCATCAAATTCGATGAACGGAATTATGGATATAGATACTGTTACATTTCATGATAAAGATATATCAGTACTTAAGCAGGGAGCTGGTCAAGTTCAACCTCTTGATTCCTCACTGATGTGCAGCCTGCAACCGCAGGTCCATAATGATACTACCGGAGGATTCAACAATATGTATCACATATCGAATTCTTCCACAATACCCGACAATTGTTTTCATTCGTCAACTCCAATGAGCAACGGAGTATGTGACTCTACACAGTGTACACATCACGGTGGGTCTACGACAAATGGAGCTTCTGGCTCCGGGTATCGGTGGGGATCCTTCACCCAGCTCCATCATCAAAACTATAAGAATTACAGCATGTCCTCGGAGCAGATGCCCTCATCTTCGACGTGGTATCCTTCTGAGCATCCCTTGCAATATCCAACAGAGATTTGGACACAATCGGAGCCTGAAAATCATGAGATGCCTCAAGTGGATGATTCAACTTCTGTATCGTTACCTGCCCGATACATAGAATATGAGTCTATGCTTGTCAACAAGGAAGAAGCTGATATCATGCCTGTTCAGCATGCCAATAAGGACGAAGCTGATGATGATGGATTACAGTTTCTTAGCATTCCAGATGAAGTTCATAAAAATGATTCCAAATTGGCGTCATTTGATGACGAAATTAACAGTGGACTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

564

Amino Acids

63.35

Weight (kDa)

5.32

Isoelectric Point (pI)

45.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 18 - 144 1.2e-38 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017690)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g17240
rosa_chinensis RchiOBHm_Chr3g0471331
rosa_laevigata RLG00000024186
rosa_multiflora Rmu_sc0010513.1_g000010 Rmu_sc0015755.1_g000003
rosa_roxburghii Rroxscaffold_6G00410050
rosa_rugosa Rorug03G0117200
rosa_samantha Rh3AG166800 Rh3BG192200 Rh3CG182500 Rh3DG183600
rosa_wichuraiana Rw3G015690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 1126, 1513
AccI GTMKAC 2 cut(s) 791, 1280
AciI CCGC 1 cut(s) 1133
AclWI GGATC 5 cut(s) 139, 152, 753, 1314, 1327
AcoI YGGCCR 1 cut(s) 266
AcsI RAATTY 3 cut(s) 368, 1009, 1186
AcyI GRCGYC 1 cut(s) 1658
AfaI GTAC 7 cut(s) 610, 636, 829, 861, 984, 1071, 1264
AfiI CCNNNNNNNGG 3 cut(s) 80, 141, 1311
AflII CTTAAG 2 cut(s) 638, 1073
AjiI CACGTC 1 cut(s) 1395
AjnI CCWGG 2 cut(s) 55, 874
AleI CACNNNNGTG 2 cut(s) 907, 1117
AluBI AGCT 7 cut(s) 184, 281, 1085, 1295, 1333, 1553, 1592
AluI AGCT 7 cut(s) 184, 281, 1085, 1295, 1333, 1553, 1592
Alw21I GWGCWC 1 cut(s) 870
Alw26I GTCTC 1 cut(s) 376
AlwI GGATC 5 cut(s) 139, 152, 753, 1314, 1327
AlwNI CAGNNNCTG 1 cut(s) 605
AoxI GGCC 1 cut(s) 266
ApeKI GCWGC 5 cut(s) 278, 643, 693, 912, 1122
ApoI RAATTY 3 cut(s) 368, 1009, 1186
Asp700I GAANNNNTTC 2 cut(s) 39, 450
AspS9I GGNCC 5 cut(s) 241, 709, 872, 928, 1138
AsuC2I CCSGG 1 cut(s) 1306
AsuHPI GGTGA 3 cut(s) 350, 488, 1318
AvaII GGWCC 5 cut(s) 241, 709, 872, 928, 1138
BaeI ACNNNNGTAYC 2 cut(s) 1479, 1512
BamHI GGATCC 2 cut(s) 144, 1319
BauI CACGAG 1 cut(s) 282
BbsI GAAGAC 1 cut(s) 453
Bbv12I GWGCWC 1 cut(s) 870
BbvI GCAGC 5 cut(s) 290, 655, 705, 924, 1134
BccI CCATC 5 cut(s) 74, 180, 209, 1344, 1595
BciT130I CCWGG 2 cut(s) 57, 876
BciVI GTATCC 2 cut(s) 490, 1410
BclI TGATCA 1 cut(s) 580
BcnI CCSGG 1 cut(s) 1306
BcoDI GTCTC 1 cut(s) 376
BfmI CTRYAG 1 cut(s) 567
BfrI CTTAAG 2 cut(s) 638, 1073
BfuAI ACCTGC 2 cut(s) 1126, 1513
BfuI GTATCC 2 cut(s) 490, 1410
BglII AGATCT 1 cut(s) 114
BisI GCNGC 5 cut(s) 279, 644, 694, 913, 1123
BlsI GCNGC 5 cut(s) 280, 645, 695, 914, 1124
BmcAI AGTACT 4 cut(s) 636, 829, 861, 1071
Bme1390I CCNGG 3 cut(s) 57, 876, 1306
Bme18I GGWCC 5 cut(s) 241, 709, 872, 928, 1138
BmgBI CACGTC 1 cut(s) 1395
BmgT120I GGNCC 5 cut(s) 241, 709, 872, 928, 1138
BmiI GGNNCC 6 cut(s) 146, 242, 898, 1303, 1321, 1452
BmrFI CCNGG 3 cut(s) 57, 876, 1306
BmsI GCATC 5 cut(s) 547, 592, 1368, 1420, 1458
BpiI GAAGAC 1 cut(s) 453
BpuEI CTTGAG 2 cut(s) 884, 1458
BpuMI CCSGG 1 cut(s) 1306
Bsa29I ATCGAT 1 cut(s) 64
BsaHI GRCGYC 1 cut(s) 1658
BsaJI CCNNGG 1 cut(s) 1368
BsaWI WCCGGW 3 cut(s) 725, 938, 1154
Bsc4I CCNNNNNNNGG 3 cut(s) 80, 141, 1311
Bse1I ACTGG 2 cut(s) 128, 310
Bse3DI GCAATG 1 cut(s) 541
BseBI CCWGG 2 cut(s) 57, 876
BseCI ATCGAT 1 cut(s) 64
BseDI CCNNGG 1 cut(s) 1368
BseGI GGATG 5 cut(s) 172, 442, 693, 1411, 1486
BseLI CCNNNNNNNGG 3 cut(s) 80, 141, 1311
BseMI GCAATG 1 cut(s) 541
BseMII CTCAG 1 cut(s) 1398
BseNI ACTGG 2 cut(s) 128, 310
BseXI GCAGC 5 cut(s) 290, 655, 705, 924, 1134
BseYI CCCAGC 1 cut(s) 1329
BsgI GTGCAG 3 cut(s) 297, 931, 1141
BshFI GGCC 1 cut(s) 268
BshVI ATCGAT 1 cut(s) 64
BsiHKAI GWGCWC 1 cut(s) 870
BsiSI CCGG 4 cut(s) 726, 939, 1155, 1305
BslFI GGGAC 2 cut(s) 254, 274
BslI CCNNNNNNNGG 3 cut(s) 80, 141, 1311
BsmAI GTCTC 1 cut(s) 376
BsmFI GGGAC 2 cut(s) 254, 274
BsmI GAATGC 1 cut(s) 1620
BsnI GGCC 1 cut(s) 268
Bsp1286I GDGCHC 1 cut(s) 870
Bsp1407I TGTACA 1 cut(s) 1262
Bsp143I GATC 5 cut(s) 114, 144, 580, 745, 1319
BspACI CCGC 1 cut(s) 1133
BspANI GGCC 1 cut(s) 268
BspCNI CTCAG 1 cut(s) 1399
BspDI ATCGAT 1 cut(s) 64
BspHI TCATGA 4 cut(s) 616, 841, 1051, 1462
BspLI GGNNCC 6 cut(s) 146, 242, 898, 1303, 1321, 1452
BspMI ACCTGC 2 cut(s) 1126, 1513
BspPI GGATC 5 cut(s) 139, 152, 753, 1314, 1327
BspTI CTTAAG 2 cut(s) 638, 1073
BsrDI GCAATG 1 cut(s) 541
BsrGI TGTACA 1 cut(s) 1262
BsrI ACTGG 2 cut(s) 128, 310
BssECI CCNNGG 1 cut(s) 1368
BssMI GATC 5 cut(s) 114, 144, 580, 745, 1319
BssNI GRCGYC 1 cut(s) 1658
BssSI CACGAG 1 cut(s) 282
Bst2BI CACGAG 1 cut(s) 282
Bst2UI CCWGG 2 cut(s) 57, 876
Bst6I CTCTTC 1 cut(s) 54
BstACI GRCGYC 1 cut(s) 1658
BstAFI CTTAAG 2 cut(s) 638, 1073
BstAPI GCANNNNNTGC 1 cut(s) 1417
BstAUI TGTACA 1 cut(s) 1262
BstC8I GCNNGC 4 cut(s) 698, 917, 1127, 1575
BstDEI CTNAG 2 cut(s) 1407, 1616
BstF5I GGATG 5 cut(s) 172, 442, 693, 1411, 1486
BstKTI GATC 5 cut(s) 117, 147, 583, 748, 1322
BstMAI GTCTC 1 cut(s) 376
BstMBI GATC 5 cut(s) 114, 144, 580, 745, 1319
BstMWI GCNNNNNNNGC 2 cut(s) 1417, 1570
BstNI CCWGG 2 cut(s) 57, 876
BstNSI RCATGY 3 cut(s) 700, 1366, 1577
BstSCI CCNGG 3 cut(s) 55, 874, 1304
BstSFI CTRYAG 1 cut(s) 567
BstV1I GCAGC 5 cut(s) 290, 655, 705, 924, 1134
BstV2I GAAGAC 1 cut(s) 453
BstX2I RGATCY 3 cut(s) 114, 144, 1319
BstYI RGATCY 3 cut(s) 114, 144, 1319
Bsu15I ATCGAT 1 cut(s) 64
BsuI GTATCC 2 cut(s) 490, 1410
BsuRI GGCC 1 cut(s) 268
BsuTUI ATCGAT 1 cut(s) 64
BtrI CACGTC 1 cut(s) 1395
BtsCI GGATG 5 cut(s) 172, 442, 693, 1411, 1486
BtsIMutI CAGTG 3 cut(s) 1112, 1265, 1687
BveI ACCTGC 2 cut(s) 1126, 1513
Cac8I GCNNGC 4 cut(s) 698, 917, 1127, 1575
CaiI CAGNNNCTG 1 cut(s) 605
CciI TCATGA 4 cut(s) 616, 841, 1051, 1462
Cfr13I GGNCC 5 cut(s) 241, 709, 872, 928, 1138
ClaI ATCGAT 1 cut(s) 64
CseI GACGC 1 cut(s) 1647
CsiI ACCWGGT 2 cut(s) 55, 874
Csp6I GTAC 7 cut(s) 609, 635, 828, 860, 983, 1070, 1263
CviQI GTAC 7 cut(s) 609, 635, 828, 860, 983, 1070, 1263
DdeI CTNAG 2 cut(s) 1407, 1616
DpnI GATC 5 cut(s) 116, 146, 582, 747, 1321
DpnII GATC 5 cut(s) 114, 144, 580, 745, 1319
DraI TTTAAA 2 cut(s) 373, 522
EaeI YGGCCR 1 cut(s) 266
Eam1104I CTCTTC 1 cut(s) 54
EarI CTCTTC 1 cut(s) 54
Eco32I GATATC 1 cut(s) 1558
Eco47I GGWCC 5 cut(s) 241, 709, 872, 928, 1138
EcoRI GAATTC 1 cut(s) 1186
EcoRII CCWGG 2 cut(s) 55, 874
EcoRV GATATC 1 cut(s) 1558
EcoT22I ATGCAT 1 cut(s) 420
FaqI GGGAC 2 cut(s) 254, 274
FbaI TGATCA 1 cut(s) 580
FblI GTMKAC 2 cut(s) 791, 1280
Fnu4HI GCNGC 5 cut(s) 279, 644, 694, 913, 1123
FokI GGATG 5 cut(s) 159, 449, 700, 1398, 1493
Fsp4HI GCNGC 5 cut(s) 279, 644, 694, 913, 1123
GluI GCNGC 5 cut(s) 279, 644, 694, 913, 1123
GsaI CCCAGC 1 cut(s) 1333
HaeIII GGCC 1 cut(s) 268
HapII CCGG 4 cut(s) 726, 939, 1155, 1305
HgaI GACGC 1 cut(s) 1647
Hin1I GRCGYC 1 cut(s) 1658
HincII GTYRAC 4 cut(s) 792, 881, 1224, 1540
HindII GTYRAC 4 cut(s) 792, 881, 1224, 1540
HpaII CCGG 4 cut(s) 726, 939, 1155, 1305
HphI GGTGA 3 cut(s) 350, 488, 1318
Hpy188I TCNGA 4 cut(s) 580, 1372, 1408, 1450
Hpy99I CGWCG 2 cut(s) 793, 1396
HpyAV CCTTC 5 cut(s) 292, 436, 473, 1333, 1413
HpyCH4IV ACGT 2 cut(s) 292, 1394
HpyF10VI GCNNNNNNNGC 2 cut(s) 1417, 1570
HpyF3I CTNAG 2 cut(s) 1407, 1616
HpySE526I ACGT 2 cut(s) 292, 1394
Hsp92I GRCGYC 1 cut(s) 1658
Ksp22I TGATCA 1 cut(s) 580
Kzo9I GATC 5 cut(s) 114, 144, 580, 745, 1319
LmnI GCTCC 6 cut(s) 1082, 1292, 1307, 1338, 1372, 1450
Lsp1109I GCAGC 5 cut(s) 290, 655, 705, 924, 1134
LweI GCATC 5 cut(s) 547, 592, 1368, 1420, 1458
MabI ACCWGGT 2 cut(s) 55, 874
MaeII ACGT 2 cut(s) 292, 1394
MaeIII GTNAC 9 cut(s) 137, 562, 595, 803, 817, 832, 1042, 1247, 1500
MalI GATC 5 cut(s) 116, 146, 582, 747, 1321
MboI GATC 5 cut(s) 114, 144, 580, 745, 1319
MboII GAAGA 8 cut(s) 41, 224, 458, 753, 966, 1182, 1380, 1559
MfeI CAATTG 1 cut(s) 1207
MflI RGATCY 3 cut(s) 114, 144, 1319
MhlI GDGCHC 1 cut(s) 870
MlyI GAGTC 3 cut(s) 256, 1244, 1535
MmeI TCCRAC 3 cut(s) 275, 474, 1451
Mph1103I ATGCAT 1 cut(s) 420
MroXI GAANNNNTTC 2 cut(s) 39, 450
MseI TTAA 7 cut(s) 372, 432, 521, 639, 683, 1074, 1677
MslI CAYNNNNRTG 5 cut(s) 621, 907, 1117, 1272, 1641
MspCI CTTAAG 2 cut(s) 638, 1073
MspI CCGG 4 cut(s) 726, 939, 1155, 1305
MspR9I CCNGG 3 cut(s) 57, 876, 1306
MunI CAATTG 1 cut(s) 1207
Mva1269I GAATGC 1 cut(s) 1620
MvaI CCWGG 2 cut(s) 57, 876
MwoI GCNNNNNNNGC 2 cut(s) 1417, 1570
NciI CCSGG 1 cut(s) 1306
NdeII GATC 5 cut(s) 114, 144, 580, 745, 1319
NlaIV GGNNCC 6 cut(s) 146, 242, 898, 1303, 1321, 1452
NmeAIII GCCGAG 1 cut(s) 244
NmuCI GTSAC 2 cut(s) 562, 1247
NsiI ATGCAT 1 cut(s) 420
NspI RCATGY 3 cut(s) 700, 1366, 1577
OliI CACNNNNGTG 2 cut(s) 907, 1117
PaeI GCATGC 2 cut(s) 700, 1577
PagI TCATGA 4 cut(s) 616, 841, 1051, 1462
PcsI WCGNNNNNNNCGW 4 cut(s) 271, 280, 289, 794
PctI GAATGC 1 cut(s) 1620
PdmI GAANNNNTTC 2 cut(s) 39, 450
PflFI GACNNNGTC 1 cut(s) 876
PkrI GCNGC 5 cut(s) 280, 645, 695, 914, 1124
PleI GAGTC 3 cut(s) 256, 1244, 1534
PpsI GAGTC 3 cut(s) 256, 1244, 1534
Psp6I CCWGG 2 cut(s) 55, 874
PspFI CCCAGC 1 cut(s) 1329
PspGI CCWGG 2 cut(s) 55, 874
PspN4I GGNNCC 6 cut(s) 146, 242, 898, 1303, 1321, 1452
PspPI GGNCC 5 cut(s) 241, 709, 872, 928, 1138
PstNI CAGNNNCTG 1 cut(s) 605
PsuI RGATCY 3 cut(s) 114, 144, 1319
PsyI GACNNNGTC 1 cut(s) 876
RsaI GTAC 7 cut(s) 610, 636, 829, 861, 984, 1071, 1264
RsaNI GTAC 7 cut(s) 609, 635, 828, 860, 983, 1070, 1263
RseI CAYNNNNRTG 5 cut(s) 621, 907, 1117, 1272, 1641
SalI GTCGAC 1 cut(s) 790
SaqAI TTAA 7 cut(s) 372, 432, 521, 639, 683, 1074, 1677
SatI GCNGC 5 cut(s) 279, 644, 694, 913, 1123
Sau3AI GATC 5 cut(s) 114, 144, 580, 745, 1319
Sau96I GGNCC 5 cut(s) 241, 709, 872, 928, 1138
ScaI AGTACT 4 cut(s) 636, 829, 861, 1071
SchI GAGTC 3 cut(s) 256, 1244, 1535
ScrFI CCNGG 3 cut(s) 57, 876, 1306
SduI GDGCHC 1 cut(s) 870
SexAI ACCWGGT 2 cut(s) 55, 874
SfaNI GCATC 5 cut(s) 547, 592, 1368, 1420, 1458
SfcI CTRYAG 1 cut(s) 567
SinI GGWCC 5 cut(s) 241, 709, 872, 928, 1138
SmiI ATTTAAAT 1 cut(s) 522
SmiMI CAYNNNNRTG 5 cut(s) 621, 907, 1117, 1272, 1641
SmlI CTYRAG 4 cut(s) 638, 863, 1073, 1473
SmoI CTYRAG 4 cut(s) 638, 863, 1073, 1473
SphI GCATGC 2 cut(s) 700, 1577
SsiI CCGC 1 cut(s) 1133
SspI AATATT 2 cut(s) 38, 160
StyD4I CCNGG 3 cut(s) 55, 874, 1304
SwaI ATTTAAAT 1 cut(s) 522
TaiI ACGT 2 cut(s) 295, 1397
TaqI TCGA 8 cut(s) 64, 198, 366, 791, 797, 1013, 1184, 1391
TaqII GACCGA 1 cut(s) 258
TatI WGTACW 5 cut(s) 634, 827, 859, 1069, 1262
Tru1I TTAA 7 cut(s) 372, 432, 521, 639, 683, 1074, 1677
Tru9I TTAA 7 cut(s) 372, 432, 521, 639, 683, 1074, 1677
TscAI CASTG 3 cut(s) 1119, 1265, 1687
TseFI GTSAC 2 cut(s) 562, 1247
TseI GCWGC 5 cut(s) 278, 643, 693, 912, 1122
Tsp45I GTSAC 2 cut(s) 562, 1247
TspGWI ACGGA 4 cut(s) 822, 885, 1035, 1254
TspRI CASTG 3 cut(s) 1119, 1265, 1687
Tth111I GACNNNGTC 1 cut(s) 876
Vha464I CTTAAG 2 cut(s) 638, 1073
VpaK11BI GGWCC 5 cut(s) 241, 709, 872, 928, 1138
XapI RAATTY 3 cut(s) 368, 1009, 1186
XceI RCATGY 3 cut(s) 700, 1366, 1577
XcmI CCANNNNNNNNNTGG 1 cut(s) 1434
XmiI GTMKAC 2 cut(s) 791, 1280
XmnI GAANNNNTTC 2 cut(s) 39, 450
ZrmI AGTACT 4 cut(s) 636, 829, 861, 1071
Zsp2I ATGCAT 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.