Rh3CG182500

Pistil-specific extensin-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0017690
Physical Location & Seq
Reverse (-)
16053983 .. 16056118
2136 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG182500.1

Sequence Viewer

Length: 1497 bp
ATGTTCGACATAATTAGAGAAGATGGCTGGTATTTTTTCAGTAAAAGGGACCGAAAGTATCTAAATGGGACTCGGCCAAATCGTGCAGCTCGTGGTGGACGTTGGAAGGCAACTGGAAGTGAAGTAAATATCATTGATGAAAATGGTGAACTAATTGGACACAAAAAAGTTCTCGAATTTAAAAAAGGGAGACATAAACAAGATATAAAGACGGAATGGAAAATGCATGAATATAGAATTAAGGATGAGAAGGAAGACATTCCTCGTCCAAAGAGGAAAAAAGGTGAAGGAAATATGCAGTTGGATACTGTTTTGTGCAAAATCTATTTAAATACCAGAGGCAATGGTAATACGGATGAGAAAGATGCAGTCACTACAGAAACTTCTGATCAAGGAATTATGGTTACAGATGCTGGTACATCTCATGACAATGATATATCAGTACTTAAGCAGGGAGTTGGTCAAGTTCAACCTCTTGATTCATTATTAATGATGAGCAGCATGCAACCACAGGTCCATAATAATACTACCGGAGGATTCAACAATGTGGATCGCATACCCAATTCTTCCACAATACCCGATTGTTTTTATTCGTCGACTTCGATGAATAACGGAATTATGGTTACAGACACTGTTACATCTCATGACAAAGATATATCAGTACTTGAGCACGGACCTGGTCAACTTCAACCTCTTGGTTCCTCACTGATGTGCAGCCTGCAACCACAGGTCCATACTATCGGAGGATTCAAGAATATGGATCACATATCCAATTCCTCCACGGTACCCAACATTTGTTTTTATTCATCAAATTCGATGAACGGAAATATGGTTACAGATACTGTTACATTTCATGATAAAGATATATCGGTACTTAAGCAGGGAGCTGGTCAAGTTCAACCTCTTGGTTCCTCACTGATGTGCAGCCTGCAACCGCAGGTCCATAATAATACTACCGGAGGATTCAACAATATGTATCACATATCCAATTCTTCCACAATACCCGACAATTGTTTTTATTCGTCAACTCCAATGAGCAACGGAGTATGTGACTCTACACAGTGTACGCATCATGGTGGGTCTATGACAAATGGAGCTTCTGGCTCCGGTTATCGGTGGGGATCCTTCACCCATCTCCATCATCAAAACTATAAGAATTACAGCATGTCCTCGGAGCAGATGCCCTCATCTTCGACGTGGTATCCTTCTGAGCATCCCTTGCAATATCCAACAGAGATTTGGACACAATCGGAGCCTGAAAATCATGAGATGCCTCAAGTGGATGATTCAACTTCAGTATCGTTACCTGCCCGATACATAGAATATGAGTCTATGCTTGTCAACAAGGAAGAAGCTGATATCATGCCTGTTCAGCATGCCAATAAGGACGAAGCTGATGATGATGGATTACAGTTTCTTAGCATTCCAGATGAAGTTCATAAAAATGATTCCAAATTGGCGTCATTTGATGACGAAATTAACAGTGGACTTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pollen_Ole_e_I

Protein Analysis

498

Amino Acids

55.5

Weight (kDa)

5.34

Isoelectric Point (pI)

42.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 6 - 80 7.1e-20 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017690)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g17240
rosa_chinensis RchiOBHm_Chr3g0471331
rosa_laevigata RLG00000024186
rosa_multiflora Rmu_sc0010513.1_g000010 Rmu_sc0015755.1_g000003
rosa_roxburghii Rroxscaffold_6G00410050
rosa_rugosa Rorug03G0117200
rosa_samantha Rh3AG166800 Rh3BG192200 Rh3CG182500 Rh3DG183600
rosa_wichuraiana Rw3G015690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 928, 1315
Acc65I GGTACC 1 cut(s) 784
AccB1I GGYRCC 1 cut(s) 784
AccI GTMKAC 1 cut(s) 596
AciI CCGC 1 cut(s) 935
AclWI GGATC 4 cut(s) 558, 768, 1116, 1129
AcoI YGGCCR 1 cut(s) 74
AcsI RAATTY 2 cut(s) 176, 811
AcuI CTGAAG 1 cut(s) 1278
AcyI GRCGYC 1 cut(s) 1460
AfaI GTAC 6 cut(s) 418, 444, 663, 786, 873, 1066
AfiI CCNNNNNNNGG 1 cut(s) 1113
AflII CTTAAG 2 cut(s) 446, 875
AgsI TTSAA 7 cut(s) 470, 541, 689, 751, 899, 967, 1290
AjiI CACGTC 1 cut(s) 1197
AjnI CCWGG 1 cut(s) 676
AleI CACNNNNGTG 2 cut(s) 709, 919
AluBI AGCT 5 cut(s) 89, 887, 1097, 1355, 1394
AluI AGCT 5 cut(s) 89, 887, 1097, 1355, 1394
Alw21I GWGCWC 1 cut(s) 672
Alw26I GTCTC 1 cut(s) 184
AlwI GGATC 4 cut(s) 558, 768, 1116, 1129
AlwNI CAGNNNCTG 3 cut(s) 413, 632, 842
AoxI GGCC 1 cut(s) 74
ApeKI GCWGC 4 cut(s) 86, 498, 714, 924
ApoI RAATTY 2 cut(s) 176, 811
AseI ATTAAT 1 cut(s) 488
Asp700I GAANNNNTTC 1 cut(s) 258
Asp718I GGTACC 1 cut(s) 784
AspS9I GGNCC 5 cut(s) 49, 514, 674, 730, 940
AsuHPI GGTGA 3 cut(s) 158, 296, 1120
AvaII GGWCC 5 cut(s) 49, 514, 674, 730, 940
BaeI ACNNNNGTAYC 2 cut(s) 1281, 1314
BamHI GGATCC 1 cut(s) 1121
BanI GGYRCC 1 cut(s) 784
BauI CACGAG 1 cut(s) 90
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 672
BbvI GCAGC 4 cut(s) 98, 510, 726, 936
BccI CCATC 4 cut(s) 17, 1140, 1146, 1397
BciT130I CCWGG 1 cut(s) 678
BciVI GTATCC 2 cut(s) 298, 1212
BclI TGATCA 1 cut(s) 388
BcoDI GTCTC 1 cut(s) 184
BfmI CTRYAG 1 cut(s) 375
BfrI CTTAAG 2 cut(s) 446, 875
BfuAI ACCTGC 2 cut(s) 928, 1315
BfuI GTATCC 2 cut(s) 298, 1212
BisI GCNGC 4 cut(s) 87, 499, 715, 925
BlsI GCNGC 4 cut(s) 88, 500, 716, 926
BmcAI AGTACT 2 cut(s) 444, 663
Bme1390I CCNGG 1 cut(s) 678
Bme18I GGWCC 5 cut(s) 49, 514, 674, 730, 940
BmgBI CACGTC 1 cut(s) 1197
BmgT120I GGNCC 5 cut(s) 49, 514, 674, 730, 940
BmiI GGNNCC 7 cut(s) 50, 700, 786, 910, 1105, 1123, 1254
BmrFI CCNGG 1 cut(s) 678
BmsI GCATC 6 cut(s) 355, 400, 1078, 1170, 1222, 1260
BpiI GAAGAC 1 cut(s) 261
BpuEI CTTGAG 2 cut(s) 686, 1260
BsaHI GRCGYC 1 cut(s) 1460
BsaJI CCNNGG 2 cut(s) 780, 1170
BsaWI WCCGGW 3 cut(s) 530, 956, 1106
Bsc4I CCNNNNNNNGG 1 cut(s) 1113
Bse1I ACTGG 1 cut(s) 118
Bse3DI GCAATG 1 cut(s) 349
BseBI CCWGG 1 cut(s) 678
BseDI CCNNGG 2 cut(s) 780, 1170
BseGI GGATG 4 cut(s) 250, 361, 1213, 1288
BseLI CCNNNNNNNGG 1 cut(s) 1113
BseMI GCAATG 1 cut(s) 349
BseMII CTCAG 1 cut(s) 1200
BseNI ACTGG 1 cut(s) 118
BseXI GCAGC 4 cut(s) 98, 510, 726, 936
BsgI GTGCAG 3 cut(s) 105, 733, 943
BshFI GGCC 1 cut(s) 76
BshNI GGYRCC 1 cut(s) 784
BsiHKAI GWGCWC 1 cut(s) 672
BsiSI CCGG 3 cut(s) 531, 957, 1107
BslFI GGGAC 2 cut(s) 62, 82
BslI CCNNNNNNNGG 1 cut(s) 1113
BsmAI GTCTC 1 cut(s) 184
BsmFI GGGAC 2 cut(s) 62, 82
BsmI GAATGC 1 cut(s) 1422
BsnI GGCC 1 cut(s) 76
Bsp1286I GDGCHC 1 cut(s) 672
Bsp143I GATC 4 cut(s) 388, 550, 760, 1121
BspACI CCGC 1 cut(s) 935
BspANI GGCC 1 cut(s) 76
BspCNI CTCAG 1 cut(s) 1201
BspHI TCATGA 4 cut(s) 424, 643, 853, 1264
BspLI GGNNCC 7 cut(s) 50, 700, 786, 910, 1105, 1123, 1254
BspMI ACCTGC 2 cut(s) 928, 1315
BspPI GGATC 4 cut(s) 558, 768, 1116, 1129
BspT107I GGYRCC 1 cut(s) 784
BspTI CTTAAG 2 cut(s) 446, 875
BsrDI GCAATG 1 cut(s) 349
BsrI ACTGG 1 cut(s) 118
BssECI CCNNGG 2 cut(s) 780, 1170
BssMI GATC 4 cut(s) 388, 550, 760, 1121
BssNI GRCGYC 1 cut(s) 1460
BssSI CACGAG 1 cut(s) 90
Bst2BI CACGAG 1 cut(s) 90
Bst2UI CCWGG 1 cut(s) 678
Bst4CI ACNGT 7 cut(s) 310, 634, 784, 844, 1062, 1413, 1484
BstACI GRCGYC 1 cut(s) 1460
BstAFI CTTAAG 2 cut(s) 446, 875
BstAPI GCANNNNNTGC 1 cut(s) 1219
BstC8I GCNNGC 4 cut(s) 503, 719, 929, 1377
BstDEI CTNAG 2 cut(s) 1209, 1418
BstDSI CCRYGG 1 cut(s) 780
BstF5I GGATG 4 cut(s) 250, 361, 1213, 1288
BstKTI GATC 4 cut(s) 391, 553, 763, 1124
BstMAI GTCTC 1 cut(s) 184
BstMBI GATC 4 cut(s) 388, 550, 760, 1121
BstMWI GCNNNNNNNGC 2 cut(s) 1219, 1372
BstNI CCWGG 1 cut(s) 678
BstNSI RCATGY 3 cut(s) 505, 1168, 1379
BstSCI CCNGG 1 cut(s) 676
BstSFI CTRYAG 1 cut(s) 375
BstV1I GCAGC 4 cut(s) 98, 510, 726, 936
BstV2I GAAGAC 1 cut(s) 261
BstX2I RGATCY 1 cut(s) 1121
BstYI RGATCY 1 cut(s) 1121
BsuI GTATCC 2 cut(s) 298, 1212
BsuRI GGCC 1 cut(s) 76
BtgI CCRYGG 1 cut(s) 780
BtrI CACGTC 1 cut(s) 1197
BtsCI GGATG 4 cut(s) 250, 361, 1213, 1288
BtsIMutI CAGTG 5 cut(s) 630, 704, 914, 1067, 1489
BveI ACCTGC 2 cut(s) 928, 1315
Cac8I GCNNGC 4 cut(s) 503, 719, 929, 1377
CaiI CAGNNNCTG 3 cut(s) 413, 632, 842
CciI TCATGA 4 cut(s) 424, 643, 853, 1264
Cfr13I GGNCC 5 cut(s) 49, 514, 674, 730, 940
CseI GACGC 1 cut(s) 1449
CsiI ACCWGGT 1 cut(s) 676
Csp6I GTAC 6 cut(s) 417, 443, 662, 785, 872, 1065
CviQI GTAC 6 cut(s) 417, 443, 662, 785, 872, 1065
DdeI CTNAG 2 cut(s) 1209, 1418
DpnI GATC 4 cut(s) 390, 552, 762, 1123
DpnII GATC 4 cut(s) 388, 550, 760, 1121
DraI TTTAAA 2 cut(s) 181, 330
EaeI YGGCCR 1 cut(s) 74
Eco32I GATATC 1 cut(s) 1360
Eco47I GGWCC 5 cut(s) 49, 514, 674, 730, 940
Eco57I CTGAAG 1 cut(s) 1278
EcoRII CCWGG 1 cut(s) 676
EcoRV GATATC 1 cut(s) 1360
EcoT22I ATGCAT 1 cut(s) 228
FaqI GGGAC 2 cut(s) 62, 82
FbaI TGATCA 1 cut(s) 388
FblI GTMKAC 1 cut(s) 596
Fnu4HI GCNGC 4 cut(s) 87, 499, 715, 925
FokI GGATG 4 cut(s) 257, 368, 1200, 1295
Fsp4HI GCNGC 4 cut(s) 87, 499, 715, 925
GluI GCNGC 4 cut(s) 87, 499, 715, 925
HaeIII GGCC 1 cut(s) 76
HapII CCGG 3 cut(s) 531, 957, 1107
HgaI GACGC 1 cut(s) 1449
Hin1I GRCGYC 1 cut(s) 1460
HincII GTYRAC 4 cut(s) 597, 683, 1026, 1342
HindII GTYRAC 4 cut(s) 597, 683, 1026, 1342
HinfI GANTC 9 cut(s) 70, 479, 537, 747, 963, 1052, 1286, 1328, 1448
HpaII CCGG 3 cut(s) 531, 957, 1107
HphI GGTGA 3 cut(s) 158, 296, 1120
Hpy166II GTNNAC 8 cut(s) 98, 149, 597, 683, 1026, 1065, 1342, 1487
Hpy188I TCNGA 5 cut(s) 388, 743, 1174, 1210, 1252
Hpy188III TCNNGA 8 cut(s) 173, 425, 476, 644, 751, 854, 1265, 1427
Hpy8I GTNNAC 8 cut(s) 98, 149, 597, 683, 1026, 1065, 1342, 1487
Hpy99I CGWCG 2 cut(s) 598, 1198
HpyAV CCTTC 5 cut(s) 100, 244, 281, 1135, 1215
HpyCH4III ACNGT 7 cut(s) 310, 634, 784, 844, 1062, 1413, 1484
HpyCH4IV ACGT 2 cut(s) 100, 1196
HpyF10VI GCNNNNNNNGC 2 cut(s) 1219, 1372
HpyF3I CTNAG 2 cut(s) 1209, 1418
HpySE526I ACGT 2 cut(s) 100, 1196
Hsp92I GRCGYC 1 cut(s) 1460
KpnI GGTACC 1 cut(s) 788
Ksp22I TGATCA 1 cut(s) 388
Kzo9I GATC 4 cut(s) 388, 550, 760, 1121
LmnI GCTCC 5 cut(s) 884, 1094, 1109, 1174, 1252
Lsp1109I GCAGC 4 cut(s) 98, 510, 726, 936
LweI GCATC 6 cut(s) 355, 400, 1078, 1170, 1222, 1260
MabI ACCWGGT 1 cut(s) 676
MaeII ACGT 2 cut(s) 100, 1196
MaeIII GTNAC 8 cut(s) 370, 403, 622, 634, 832, 844, 1049, 1302
MalI GATC 4 cut(s) 390, 552, 762, 1123
MboI GATC 4 cut(s) 388, 550, 760, 1121
MboII GAAGA 6 cut(s) 32, 266, 558, 984, 1182, 1361
MfeI CAATTG 1 cut(s) 1009
MflI RGATCY 1 cut(s) 1121
MhlI GDGCHC 1 cut(s) 672
MlyI GAGTC 3 cut(s) 64, 1046, 1337
MmeI TCCRAC 3 cut(s) 83, 282, 1253
Mph1103I ATGCAT 1 cut(s) 228
MroXI GAANNNNTTC 1 cut(s) 258
MseI TTAA 7 cut(s) 180, 240, 329, 447, 488, 876, 1479
MslI CAYNNNNRTG 5 cut(s) 429, 709, 919, 1074, 1443
MspCI CTTAAG 2 cut(s) 446, 875
MspI CCGG 3 cut(s) 531, 957, 1107
MspR9I CCNGG 1 cut(s) 678
MunI CAATTG 1 cut(s) 1009
Mva1269I GAATGC 1 cut(s) 1422
MvaI CCWGG 1 cut(s) 678
MwoI GCNNNNNNNGC 2 cut(s) 1219, 1372
NdeII GATC 4 cut(s) 388, 550, 760, 1121
NlaIV GGNNCC 7 cut(s) 50, 700, 786, 910, 1105, 1123, 1254
NmeAIII GCCGAG 1 cut(s) 52
NmuCI GTSAC 2 cut(s) 370, 1049
NsiI ATGCAT 1 cut(s) 228
NspI RCATGY 3 cut(s) 505, 1168, 1379
OliI CACNNNNGTG 2 cut(s) 709, 919
PaeI GCATGC 2 cut(s) 505, 1379
PagI TCATGA 4 cut(s) 424, 643, 853, 1264
PcsI WCGNNNNNNNCGW 4 cut(s) 79, 88, 97, 599
PctI GAATGC 1 cut(s) 1422
PdmI GAANNNNTTC 1 cut(s) 258
PfeI GAWTC 6 cut(s) 479, 537, 747, 963, 1286, 1448
PflFI GACNNNGTC 1 cut(s) 678
PkrI GCNGC 4 cut(s) 88, 500, 716, 926
PleI GAGTC 3 cut(s) 64, 1046, 1336
PpsI GAGTC 3 cut(s) 64, 1046, 1336
PshBI ATTAAT 1 cut(s) 488
Psp6I CCWGG 1 cut(s) 676
PspGI CCWGG 1 cut(s) 676
PspN4I GGNNCC 7 cut(s) 50, 700, 786, 910, 1105, 1123, 1254
PspPI GGNCC 5 cut(s) 49, 514, 674, 730, 940
PstNI CAGNNNCTG 3 cut(s) 413, 632, 842
PsuI RGATCY 1 cut(s) 1121
PsyI GACNNNGTC 1 cut(s) 678
RsaI GTAC 6 cut(s) 418, 444, 663, 786, 873, 1066
RsaNI GTAC 6 cut(s) 417, 443, 662, 785, 872, 1065
RseI CAYNNNNRTG 5 cut(s) 429, 709, 919, 1074, 1443
SalI GTCGAC 1 cut(s) 595
SaqAI TTAA 7 cut(s) 180, 240, 329, 447, 488, 876, 1479
SatI GCNGC 4 cut(s) 87, 499, 715, 925
Sau3AI GATC 4 cut(s) 388, 550, 760, 1121
Sau96I GGNCC 5 cut(s) 49, 514, 674, 730, 940
ScaI AGTACT 2 cut(s) 444, 663
SchI GAGTC 3 cut(s) 64, 1046, 1337
ScrFI CCNGG 1 cut(s) 678
SduI GDGCHC 1 cut(s) 672
SexAI ACCWGGT 1 cut(s) 676
SfaNI GCATC 6 cut(s) 355, 400, 1078, 1170, 1222, 1260
SfcI CTRYAG 1 cut(s) 375
SinI GGWCC 5 cut(s) 49, 514, 674, 730, 940
SmiI ATTTAAAT 1 cut(s) 330
SmiMI CAYNNNNRTG 5 cut(s) 429, 709, 919, 1074, 1443
SmlI CTYRAG 4 cut(s) 446, 665, 875, 1275
SmoI CTYRAG 4 cut(s) 446, 665, 875, 1275
SphI GCATGC 2 cut(s) 505, 1379
SsiI CCGC 1 cut(s) 935
StyD4I CCNGG 1 cut(s) 676
SwaI ATTTAAAT 1 cut(s) 330
TaaI ACNGT 7 cut(s) 310, 634, 784, 844, 1062, 1413, 1484
TaiI ACGT 2 cut(s) 103, 1199
TaqI TCGA 6 cut(s) 6, 174, 596, 602, 815, 1193
TaqII GACCGA 1 cut(s) 66
TatI WGTACW 2 cut(s) 442, 661
TfiI GAWTC 6 cut(s) 479, 537, 747, 963, 1286, 1448
Tru1I TTAA 7 cut(s) 180, 240, 329, 447, 488, 876, 1479
Tru9I TTAA 7 cut(s) 180, 240, 329, 447, 488, 876, 1479
TscAI CASTG 5 cut(s) 637, 711, 921, 1067, 1489
TseFI GTSAC 2 cut(s) 370, 1049
TseI GCWGC 4 cut(s) 86, 498, 714, 924
Tsp45I GTSAC 2 cut(s) 370, 1049
TspDTI ATGAA 9 cut(s) 153, 243, 471, 620, 795, 833, 842, 1427, 1446
TspGWI ACGGA 6 cut(s) 227, 368, 627, 687, 837, 1056
TspRI CASTG 5 cut(s) 637, 711, 921, 1067, 1489
Tth111I GACNNNGTC 1 cut(s) 678
Vha464I CTTAAG 2 cut(s) 446, 875
VpaK11BI GGWCC 5 cut(s) 49, 514, 674, 730, 940
VspI ATTAAT 1 cut(s) 488
XapI RAATTY 2 cut(s) 176, 811
XceI RCATGY 3 cut(s) 505, 1168, 1379
XcmI CCANNNNNNNNNTGG 1 cut(s) 1236
XmiI GTMKAC 1 cut(s) 596
XmnI GAANNNNTTC 1 cut(s) 258
ZrmI AGTACT 2 cut(s) 444, 663
Zsp2I ATGCAT 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.