FvH4_6g17630

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012637
Physical Location & Seq
Forward (+)
11533663 .. 11536661
2999 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g17630.t3

Sequence Viewer

Length: 1179 bp
ATGGAAGAAAAGGAAAGCTCCTTCTCTGGCTCACTCGGCAACTCCGACACCGACTCACCCCCACCCAACTCCTTCAACCACACCCTCGACGTCTCGTCTCAGGTGGTCAACACGGCGGTCAACGTCACCTCAGACACCACACTACTCGTTCCCACTCCAACAGCACCACCACCAACAACAACAACAACAGTTGCAGCAGTTACAACAGCACAAGCACTAGTTGCACCAAACACAGGTGGTGTTGTTGGAGTTCAAGGAAATGTTGACTTGTCTGGGAAGAAGAAGAGAGGGAGACCCAGAAAGTACGATGCTGATGGGAACTTGAGGCCGGGCCAGACCCACAACTCGACTCACGTGACTCACGTGTCGCCGCCGCCAGGGTTTTATCTCTCGCCGGGTTCTCCCAATCCTTCAGAGTTTTCTGTAAAGAAGGGAAGAGGAAGGCCGGCTGGATCCGGCAACTGGCAGGTTCTTGCTTCTTTAGATACAGCAGGTAACGACTTCACACCACATGTGGTTACTGTTGGCACTGGAGAGGATGTTGCAGGGAGAATTCTTTTGTTTTCTGAGAAGGGTCCTCGAGGGATTTGCATTCTCTCTGCTAATGGAGCTGTCTCAAATGTCACCATTCGCCAACCAGGTTCTACTGGTGGTATTTTGACTTATGAGGGCCGTTTTGAGTTGTTGTCTTTATCTGGGTCGTTTACAGTCACTGAGATTGGTGGCATAAGAAGCCGGACTGGCGGCTTGAGTGTCTCATTGGCTGGACCGGATGGTCGGGTGATTGGTGGTAGCATTGCTGGTATGCTAACAGCTGCGAGTCCTATCCAAATTGTGGTTGGAAGCTTTATGCCGAATGGCTGTAAGCCACAAAAGAAGAAATATCATCGTGAGAACACAGTGGCTTCTCCAATATCAGGTGCACCAGATACAGTCACCGCAGCAACACCTATCTCGCAAGCACAACCTGAAACTGAGAACAGGTTTACCCCAATAGTCCATTTACCAGCACAAAGCCATGGAGAATCAGATAAAGGCATGAGCCAAAACCAGAACTTCAATTTCACACCCACTAGTGCTCCCATTTTCATGCCTACTAATGCTGGTTGGAATGGAATGGAGTTGAAGTTGGAACATAGGCCATCTCCAGACATTAATCTATCTGTTCCGGGTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook DUF296

Protein Analysis

393

Amino Acids

40.65

Weight (kDa)

7.84

Isoelectric Point (pI)

34.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 169 - 278 8.9e-21 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 364, 774
AatII GACGTC 1 cut(s) 93
AbsI CCTCGAGG 1 cut(s) 579
Acc36I ACCTGC 2 cut(s) 457, 482
AccB7I CCANNNNNTGG 1 cut(s) 835
AciI CCGC 5 cut(s) 116, 371, 374, 744, 939
AclWI GGATC 2 cut(s) 447, 460
AcsI RAATTY 1 cut(s) 552
AcuI CTGAAG 1 cut(s) 396
AcvI CACGTG 2 cut(s) 355, 364
AcyI GRCGYC 1 cut(s) 90
AfaI GTAC 1 cut(s) 305
AfiI CCNNNNNNNGG 5 cut(s) 233, 377, 462, 835, 917
AflIII ACRYGT 2 cut(s) 363, 511
AgsI TTSAA 4 cut(s) 76, 254, 1060, 1126
AhdI GACNNNNNGTC 1 cut(s) 94
AhlI ACTAGT 2 cut(s) 217, 1073
AjnI CCWGG 2 cut(s) 376, 637
AluBI AGCT 4 cut(s) 18, 611, 815, 846
AluI AGCT 4 cut(s) 18, 611, 815, 846
Alw21I GWGCWC 2 cut(s) 925, 1081
Alw26I GTCTC 5 cut(s) 97, 102, 286, 619, 760
Alw44I GTGCAC 1 cut(s) 921
AlwI GGATC 2 cut(s) 447, 460
AlwNI CAGNNNCTG 1 cut(s) 713
Ama87I CYCGRG 1 cut(s) 579
AoxI GGCC 5 cut(s) 326, 331, 443, 670, 1139
ApaLI GTGCAC 1 cut(s) 921
ApeKI GCWGC 3 cut(s) 194, 815, 941
ApoI RAATTY 1 cut(s) 552
AseI ATTAAT 1 cut(s) 1155
AspS9I GGNCC 4 cut(s) 331, 575, 670, 767
AsuC2I CCSGG 3 cut(s) 330, 396, 1170
AsuHPI GGTGA 5 cut(s) 48, 118, 616, 793, 928
AvaI CYCGRG 1 cut(s) 579
AvaII GGWCC 2 cut(s) 575, 767
BaeGI GKGCMC 1 cut(s) 925
BamHI GGATCC 1 cut(s) 452
BbrPI CACGTG 2 cut(s) 355, 364
Bbv12I GWGCWC 2 cut(s) 925, 1081
BbvI GCAGC 3 cut(s) 206, 802, 953
BccI CCATC 3 cut(s) 308, 767, 1150
BceAI ACGGC 2 cut(s) 129, 657
BcgI CGANNNNNNTGC 2 cut(s) 570, 604
BciT130I CCWGG 2 cut(s) 378, 639
BcnI CCSGG 3 cut(s) 330, 396, 1170
BcoDI GTCTC 5 cut(s) 97, 102, 286, 619, 760
BcuI ACTAGT 2 cut(s) 217, 1073
BfaI CTAG 2 cut(s) 218, 1074
BfuAI ACCTGC 2 cut(s) 457, 482
BglI GCCNNNNNGGC 1 cut(s) 741
BisI GCNGC 6 cut(s) 195, 371, 374, 745, 816, 942
BlsI GCNGC 6 cut(s) 196, 372, 375, 746, 817, 943
Bme1390I CCNGG 5 cut(s) 330, 378, 396, 639, 1170
Bme18I GGWCC 2 cut(s) 575, 767
BmeRI GACNNNNNGTC 1 cut(s) 94
BmeT110I CYCGRG 1 cut(s) 579
BmgT120I GGNCC 4 cut(s) 331, 575, 670, 767
BmiI GGNNCC 2 cut(s) 454, 576
BmrFI CCNGG 5 cut(s) 330, 378, 396, 639, 1170
BmsI GCATC 1 cut(s) 298
BpmI CTGGAG 2 cut(s) 552, 1131
BpuEI CTTGAG 2 cut(s) 343, 769
BpuMI CCSGG 3 cut(s) 330, 396, 1170
BsaAI YACGTR 2 cut(s) 355, 364
BsaHI GRCGYC 1 cut(s) 90
BsaI GGTCTC 1 cut(s) 286
BsaJI CCNNGG 2 cut(s) 377, 1018
BsaWI WCCGGW 1 cut(s) 769
BsaXI ACNNNNNCTCC 6 cut(s) 525, 555, 1063, 1093, 1112, 1142
Bsc4I CCNNNNNNNGG 5 cut(s) 233, 377, 462, 835, 917
Bse118I RCCGGY 1 cut(s) 445
Bse1I ACTGG 4 cut(s) 467, 535, 652, 745
Bse3DI GCAATG 1 cut(s) 795
BseBI CCWGG 2 cut(s) 378, 639
BseDI CCNNGG 2 cut(s) 377, 1018
BseGI GGATG 2 cut(s) 544, 778
BseLI CCNNNNNNNGG 5 cut(s) 233, 377, 462, 835, 917
BseMI GCAATG 1 cut(s) 795
BseMII CTCAG 5 cut(s) 113, 144, 558, 705, 966
BseNI ACTGG 4 cut(s) 467, 535, 652, 745
BseSI GKGCMC 1 cut(s) 925
BseXI GCAGC 3 cut(s) 206, 802, 953
BshFI GGCC 5 cut(s) 328, 333, 445, 672, 1141
BsiHKAI GWGCWC 2 cut(s) 925, 1081
BsiHKCI CYCGRG 1 cut(s) 579
BsiSI CCGG 7 cut(s) 329, 395, 446, 456, 736, 770, 1169
BslI CCNNNNNNNGG 5 cut(s) 233, 377, 462, 835, 917
BsmAI GTCTC 5 cut(s) 97, 102, 286, 619, 760
BsmBI CGTCTC 2 cut(s) 97, 102
BsmI GAATGC 1 cut(s) 591
BsnI GGCC 5 cut(s) 328, 333, 445, 672, 1141
Bso31I GGTCTC 1 cut(s) 286
BsoBI CYCGRG 1 cut(s) 579
Bsp1286I GDGCHC 2 cut(s) 925, 1081
Bsp143I GATC 1 cut(s) 452
Bsp19I CCATGG 1 cut(s) 1018
BspACI CCGC 5 cut(s) 116, 371, 374, 744, 939
BspANI GGCC 5 cut(s) 328, 333, 445, 672, 1141
BspCNI CTCAG 5 cut(s) 112, 143, 559, 706, 967
BspLI GGNNCC 2 cut(s) 454, 576
BspMI ACCTGC 2 cut(s) 457, 482
BspPI GGATC 2 cut(s) 447, 460
BspTNI GGTCTC 1 cut(s) 286
BsrDI GCAATG 1 cut(s) 795
BsrFI RCCGGY 1 cut(s) 445
BsrI ACTGG 4 cut(s) 467, 535, 652, 745
BssAI RCCGGY 1 cut(s) 445
BssECI CCNNGG 2 cut(s) 377, 1018
BssMI GATC 1 cut(s) 452
BssNI GRCGYC 1 cut(s) 90
BssT1I CCWWGG 1 cut(s) 1018
Bst2UI CCWGG 2 cut(s) 378, 639
Bst4CI ACNGT 5 cut(s) 190, 523, 709, 901, 934
Bst6I CTCTTC 2 cut(s) 278, 430
BstACI GRCGYC 1 cut(s) 90
BstAPI GCANNNNNTGC 1 cut(s) 221
BstBAI YACGTR 2 cut(s) 355, 364
BstC8I GCNNGC 2 cut(s) 447, 960
BstDEI CTNAG 5 cut(s) 99, 130, 567, 714, 975
BstDSI CCRYGG 1 cut(s) 1018
BstF5I GGATG 2 cut(s) 544, 778
BstKTI GATC 1 cut(s) 455
BstMAI GTCTC 5 cut(s) 97, 102, 286, 619, 760
BstMBI GATC 1 cut(s) 452
BstMWI GCNNNNNNNGC 5 cut(s) 36, 221, 608, 732, 741
BstNI CCWGG 2 cut(s) 378, 639
BstNSI RCATGY 1 cut(s) 515
BstSCI CCNGG 5 cut(s) 328, 376, 394, 637, 1168
BstSLI GKGCMC 1 cut(s) 925
BstV1I GCAGC 3 cut(s) 206, 802, 953
BstX2I RGATCY 1 cut(s) 452
BstYI RGATCY 1 cut(s) 452
BsuRI GGCC 5 cut(s) 328, 333, 445, 672, 1141
BtgI CCRYGG 1 cut(s) 1018
BtsCI GGATG 2 cut(s) 544, 778
BtsIMutI CAGTG 3 cut(s) 528, 711, 906
BveI ACCTGC 2 cut(s) 457, 482
Cac8I GCNNGC 2 cut(s) 447, 960
CaiI CAGNNNCTG 1 cut(s) 713
Cfr10I RCCGGY 1 cut(s) 445
Cfr13I GGNCC 4 cut(s) 331, 575, 670, 767
CsiI ACCWGGT 1 cut(s) 637
Csp6I GTAC 1 cut(s) 304
CviAII CATG 4 cut(s) 512, 1019, 1039, 1090
CviQI GTAC 1 cut(s) 304
DdeI CTNAG 5 cut(s) 99, 130, 567, 714, 975
DpnI GATC 1 cut(s) 454
DpnII GATC 1 cut(s) 452
DrdI GACNNNNNNGTC 2 cut(s) 364, 774
DriI GACNNNNNGTC 1 cut(s) 94
DseDI GACNNNNNNGTC 2 cut(s) 364, 774
Eam1104I CTCTTC 2 cut(s) 278, 430
Eam1105I GACNNNNNGTC 1 cut(s) 94
EarI CTCTTC 2 cut(s) 278, 430
Eco130I CCWWGG 1 cut(s) 1018
Eco31I GGTCTC 1 cut(s) 286
Eco47I GGWCC 2 cut(s) 575, 767
Eco57I CTGAAG 1 cut(s) 396
Eco72I CACGTG 2 cut(s) 355, 364
Eco88I CYCGRG 1 cut(s) 579
EcoO109I RGGNCCY 1 cut(s) 575
EcoRI GAATTC 1 cut(s) 552
EcoRII CCWGG 2 cut(s) 376, 637
EcoT14I CCWWGG 1 cut(s) 1018
ErhI CCWWGG 1 cut(s) 1018
Esp3I CGTCTC 2 cut(s) 97, 102
FaeI CATG 4 cut(s) 515, 1022, 1042, 1093
FaiI YATR 9 cut(s) 513, 666, 728, 806, 851, 1020, 1040, 1091, 1137
FatI CATG 4 cut(s) 511, 1018, 1038, 1089
Fnu4HI GCNGC 6 cut(s) 195, 371, 374, 745, 816, 942
FokI GGATG 2 cut(s) 551, 785
Fsp4HI GCNGC 6 cut(s) 195, 371, 374, 745, 816, 942
FspBI CTAG 2 cut(s) 218, 1074
GluI GCNGC 6 cut(s) 195, 371, 374, 745, 816, 942
GsuI CTGGAG 2 cut(s) 552, 1131
HaeIII GGCC 5 cut(s) 328, 333, 445, 672, 1141
HapII CCGG 7 cut(s) 329, 395, 446, 456, 736, 770, 1169
Hin1I GRCGYC 1 cut(s) 90
Hin1II CATG 4 cut(s) 515, 1022, 1042, 1093
HincII GTYRAC 3 cut(s) 109, 121, 265
HindII GTYRAC 3 cut(s) 109, 121, 265
HindIII AAGCTT 1 cut(s) 844
HinfI GANTC 5 cut(s) 53, 349, 358, 820, 1025
HpaII CCGG 7 cut(s) 329, 395, 446, 456, 736, 770, 1169
HphI GGTGA 5 cut(s) 48, 118, 616, 793, 928
Hpy166II GTNNAC 6 cut(s) 109, 121, 265, 705, 923, 987
Hpy188I TCNGA 5 cut(s) 46, 133, 415, 568, 1030
Hpy188III TCNNGA 2 cut(s) 890, 1148
Hpy8I GTNNAC 6 cut(s) 109, 121, 265, 705, 923, 987
Hpy99I CGWCG 1 cut(s) 92
HpyAV CCTTC 6 cut(s) 31, 82, 420, 424, 435, 565
HpyCH4III ACNGT 5 cut(s) 190, 523, 709, 901, 934
HpyCH4IV ACGT 4 cut(s) 90, 123, 354, 363
HpyCH4V TGCA 5 cut(s) 194, 224, 545, 591, 923
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 221, 608, 732, 741
HpyF3I CTNAG 5 cut(s) 99, 130, 567, 714, 975
HpySE526I ACGT 4 cut(s) 90, 123, 354, 363
Hsp92I GRCGYC 1 cut(s) 90
Hsp92II CATG 4 cut(s) 515, 1022, 1042, 1093
KroI GCCGGC 1 cut(s) 445
KroNI GCCGGC 1 cut(s) 447
Kzo9I GATC 1 cut(s) 452
LmnI GCTCC 3 cut(s) 23, 608, 1084
Lsp1109I GCAGC 3 cut(s) 206, 802, 953
LweI GCATC 1 cut(s) 298
MabI ACCWGGT 1 cut(s) 637
MaeI CTAG 2 cut(s) 218, 1074
MaeII ACGT 4 cut(s) 90, 123, 354, 363
MaeIII GTNAC 8 cut(s) 124, 199, 355, 494, 517, 622, 709, 934
MalI GATC 1 cut(s) 454
MboI GATC 1 cut(s) 452
MboII GAAGA 6 cut(s) 17, 289, 292, 295, 447, 889
MflI RGATCY 1 cut(s) 452
MhlI GDGCHC 2 cut(s) 925, 1081
MluCI AATT 4 cut(s) 552, 831, 1060, 1174
MlyI GAGTC 4 cut(s) 47, 343, 352, 829
MmeI TCCRAC 6 cut(s) 69, 182, 226, 820, 1088, 1110
MnlI CCTC 9 cut(s) 95, 139, 281, 318, 431, 529, 575, 588, 661
MroNI GCCGGC 1 cut(s) 445
MseI TTAA 1 cut(s) 1155
MslI CAYNNNNRTG 1 cut(s) 1088
MspA1I CMGCKG 1 cut(s) 815
MspI CCGG 7 cut(s) 329, 395, 446, 456, 736, 770, 1169
MspR9I CCNGG 5 cut(s) 330, 378, 396, 639, 1170
Mva1269I GAATGC 1 cut(s) 591
MvaI CCWGG 2 cut(s) 378, 639
MwoI GCNNNNNNNGC 5 cut(s) 36, 221, 608, 732, 741
NaeI GCCGGC 1 cut(s) 447
NciI CCSGG 3 cut(s) 330, 396, 1170
NcoI CCATGG 1 cut(s) 1018
NdeII GATC 1 cut(s) 452
NgoMIV GCCGGC 1 cut(s) 445
NlaIII CATG 4 cut(s) 515, 1022, 1042, 1093
NlaIV GGNNCC 2 cut(s) 454, 576
NmeAIII GCCGAG 1 cut(s) 15
NmuCI GTSAC 5 cut(s) 124, 355, 622, 709, 934
NspI RCATGY 1 cut(s) 515
PaeR7I CTCGAG 1 cut(s) 579
PciI ACATGT 1 cut(s) 511
PcsI WCGNNNNNNNCGW 2 cut(s) 42, 360
PctI GAATGC 1 cut(s) 591
PdiI GCCGGC 1 cut(s) 447
PfeI GAWTC 1 cut(s) 1025
PflMI CCANNNNNTGG 1 cut(s) 835
PkrI GCNGC 6 cut(s) 196, 372, 375, 746, 817, 943
PleI GAGTC 4 cut(s) 47, 343, 352, 828
PmaCI CACGTG 2 cut(s) 355, 364
PmlI CACGTG 2 cut(s) 355, 364
PpsI GAGTC 4 cut(s) 47, 343, 352, 828
Ppu21I YACGTR 2 cut(s) 355, 364
PpuMI RGGWCCY 1 cut(s) 575
PscI ACATGT 1 cut(s) 511
PshBI ATTAAT 1 cut(s) 1155
Psp5II RGGWCCY 1 cut(s) 575
Psp6I CCWGG 2 cut(s) 376, 637
PspCI CACGTG 2 cut(s) 355, 364
PspGI CCWGG 2 cut(s) 376, 637
PspN4I GGNNCC 2 cut(s) 454, 576
PspPI GGNCC 4 cut(s) 331, 575, 670, 767
PspPPI RGGWCCY 1 cut(s) 575
PspXI VCTCGAGB 1 cut(s) 579
PstNI CAGNNNCTG 1 cut(s) 713
PsuI RGATCY 1 cut(s) 452
PvuII CAGCTG 1 cut(s) 815
RsaI GTAC 1 cut(s) 305
RsaNI GTAC 1 cut(s) 304
RseI CAYNNNNRTG 1 cut(s) 1088
SaqAI TTAA 1 cut(s) 1155
SatI GCNGC 6 cut(s) 195, 371, 374, 745, 816, 942
Sau3AI GATC 1 cut(s) 452
Sau96I GGNCC 4 cut(s) 331, 575, 670, 767
SchI GAGTC 4 cut(s) 47, 343, 352, 829
ScrFI CCNGG 5 cut(s) 330, 378, 396, 639, 1170
SduI GDGCHC 2 cut(s) 925, 1081
SexAI ACCWGGT 1 cut(s) 637
SfaNI GCATC 1 cut(s) 298
Sfr274I CTCGAG 1 cut(s) 579
SinI GGWCC 2 cut(s) 575, 767
SlaI CTCGAG 1 cut(s) 579
SmiMI CAYNNNNRTG 1 cut(s) 1088
SmlI CTYRAG 3 cut(s) 322, 579, 748
SmoI CTYRAG 3 cut(s) 322, 579, 748
SpeI ACTAGT 2 cut(s) 217, 1073
Sse9I AATT 4 cut(s) 552, 831, 1060, 1174
SsiI CCGC 5 cut(s) 116, 371, 374, 744, 939
SspMI CTAG 2 cut(s) 218, 1074
StyD4I CCNGG 5 cut(s) 328, 376, 394, 637, 1168
StyI CCWWGG 1 cut(s) 1018
TaaI ACNGT 5 cut(s) 190, 523, 709, 901, 934
TaiI ACGT 4 cut(s) 93, 126, 357, 366
TaqI TCGA 3 cut(s) 87, 347, 580
TasI AATT 4 cut(s) 552, 831, 1060, 1174
TauI GCSGC 3 cut(s) 373, 376, 747
TfiI GAWTC 1 cut(s) 1025
Tru1I TTAA 1 cut(s) 1155
Tru9I TTAA 1 cut(s) 1155
TscAI CASTG 3 cut(s) 535, 718, 906
TseFI GTSAC 5 cut(s) 124, 355, 622, 709, 934
TseI GCWGC 3 cut(s) 194, 815, 941
Tsp45I GTSAC 5 cut(s) 124, 355, 622, 709, 934
TspDTI ATGAA 1 cut(s) 1078
TspRI CASTG 3 cut(s) 535, 718, 906
Van91I CCANNNNNTGG 1 cut(s) 835
VneI GTGCAC 1 cut(s) 921
VpaK11BI GGWCC 2 cut(s) 575, 767
VspI ATTAAT 1 cut(s) 1155
XapI RAATTY 1 cut(s) 552
XceI RCATGY 1 cut(s) 515
XcmI CCANNNNNNNNNTGG 1 cut(s) 836
XhoI CTCGAG 1 cut(s) 579
XspI CTAG 2 cut(s) 218, 1074
ZraI GACGTC 1 cut(s) 91
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.