Rw3G016830

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0012637
Physical Location & Seq
Reverse (-)
18008691 .. 18011567
2877 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G016830.1

Sequence Viewer

Length: 1170 bp
ATGGAAGAAAAGGAAAGCACCTTTTCTGGCTCACTCGGCAACTCGGACACCGACTCACCCCCACTCAATAACTCGCTCAACCACACCGCCGTCTCGTCCCCGGTGGTCAACACGGCGGTCAACGTGACCTCGGAGACCACACTAGTTGTTGGTCCCACAGCACCAGCACCAGCACCAGCACCACCGCAACCAACAGCCCCAACATCACAAGTACTGGTTGCAGCAAACACAGGTGTTGGAGTCCAAGGAAATGTTGACTTGTCTGGGAAGAAGAAGAGAGGGAGACCCAGAAAGTACGACGCCGACGGAAACTTGAGGCCGGGCCAGACCCACAATTCGGGCCACGTGATTCACGTACCGCCGCCGCCGGGGTTTTATCTCTCGCCTGGTTCTCCCAGTCCCTCCGAGTTTTCTGCAAAGAAGGGAAGAGGAAGGCCGGCTGGATCCGGCAACTGGAAGGTTCTTGCTTCTTTAGAAACAGCAGGTGGGGACTTCACACCACATGTGGTTACTGTTGGGACTGGAGAGGATGTTGCGGGGAGAATTCTGTCATTTTCTGAGAAGGGTCCTCGAGGGATTTGCATTCTCTCCGCTAATGGAGCTGTTTCAAATGTTACCATTCGCCAACCTGGTTCTTCTGGCGGTATTCTGACTTATGAGGGGCGCTTTGAGTTGTTGTCTTTATCGGGATCGTTTACAGTCACTGAGATTGGTGGTGTAAGAAGCAGGACTGGCGGCTTGAGTGTCTCATTGGCTGGACCAGATGGTCGTGTAATTGGTGGTGGCATTGCTGGTATGCTAATAGCTGCGAGCCCTATCCAAATTGTGGTTGGAAGCTTTATGCCGAATGGGTGTAAGCCACAGAAGAAGAAGCATTATCGTGAGACAACAGTGGCTTCTCCAATCTCAGGTGCACCAGATACGGTCACTGCAGCAACACCTATCTCGCAAGCACAACCTGAAATTGAGACCAGGTTTAGCCCAATAGTCCCTTTAGCAGCACAAACCCATGGAGAAGCAGAAAAAGGCATGAGCCAAAACCAGAACTTCAATTTCATACCCACCAGTGCTTCCATTCTCATGCCTACTAATGCTGGTTGGAATGCAACGGAGCTGAAGTCAGATCATAGGCCATCTCCAGACATCAATGTATCTGTTCCGGGTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook DUF296

Protein Analysis

389

Amino Acids

39.87

Weight (kDa)

8.83

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 166 - 280 7.7e-24 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 473
AasI GACNNNNNNGTC 1 cut(s) 765
AbsI CCTCGAGG 1 cut(s) 570
Acc36I ACCTGC 1 cut(s) 473
AccB7I CCANNNNNTGG 1 cut(s) 826
AclWI GGATC 3 cut(s) 438, 451, 697
AcsI RAATTY 1 cut(s) 543
AcuI CTGAAG 1 cut(s) 1136
AcvI CACGTG 1 cut(s) 346
AcyI GRCGYC 1 cut(s) 300
AfaI GTAC 3 cut(s) 213, 296, 357
AfiI CCNNNNNNNGG 5 cut(s) 337, 368, 453, 826, 908
AflIII ACRYGT 1 cut(s) 502
AgsI TTSAA 2 cut(s) 609, 1051
AhlI ACTAGT 1 cut(s) 142
AjnI CCWGG 3 cut(s) 385, 628, 971
AluBI AGCT 4 cut(s) 602, 806, 837, 1114
AluI AGCT 4 cut(s) 602, 806, 837, 1114
Alw21I GWGCWC 1 cut(s) 916
Alw26I GTCTC 6 cut(s) 97, 128, 277, 751, 878, 962
Alw44I GTGCAC 1 cut(s) 912
AlwI GGATC 3 cut(s) 438, 451, 697
AlwNI CAGNNNCTG 1 cut(s) 704
Ama87I CYCGRG 1 cut(s) 570
AoxI GGCC 5 cut(s) 317, 322, 340, 434, 1130
ApaLI GTGCAC 1 cut(s) 912
ApeKI GCWGC 4 cut(s) 221, 806, 932, 998
ApoI RAATTY 1 cut(s) 543
ArsI GACNNNNNNTTYG 2 cut(s) 319, 351
AspLEI GCGC 1 cut(s) 666
AspS9I GGNCC 5 cut(s) 152, 322, 340, 566, 758
AsuC2I CCSGG 4 cut(s) 101, 321, 369, 1161
AsuHPI GGTGA 1 cut(s) 48
AvaI CYCGRG 1 cut(s) 570
AvaII GGWCC 3 cut(s) 152, 566, 758
BaeGI GKGCMC 1 cut(s) 916
BamHI GGATCC 1 cut(s) 443
BanII GRGCYC 1 cut(s) 815
BbrPI CACGTG 1 cut(s) 346
Bbv12I GWGCWC 1 cut(s) 916
BbvI GCAGC 4 cut(s) 233, 793, 944, 1010
BccI CCATC 2 cut(s) 758, 1141
BceAI ACGGC 2 cut(s) 74, 129
BcgI CGANNNNNNTGC 4 cut(s) 395, 429, 561, 595
BciT130I CCWGG 3 cut(s) 387, 630, 973
BcnI CCSGG 4 cut(s) 101, 321, 369, 1161
BcoDI GTCTC 6 cut(s) 97, 128, 277, 751, 878, 962
BcuI ACTAGT 1 cut(s) 142
BfaI CTAG 1 cut(s) 143
BfmI CTRYAG 1 cut(s) 930
BfoI RGCGCY 1 cut(s) 667
BfuAI ACCTGC 1 cut(s) 473
BisI GCNGC 7 cut(s) 222, 362, 365, 736, 807, 933, 999
BlsI GCNGC 7 cut(s) 223, 363, 366, 737, 808, 934, 1000
BmcAI AGTACT 1 cut(s) 213
Bme1390I CCNGG 7 cut(s) 101, 321, 369, 387, 630, 973, 1161
Bme18I GGWCC 3 cut(s) 152, 566, 758
BmeT110I CYCGRG 1 cut(s) 570
BmgT120I GGNCC 5 cut(s) 152, 322, 340, 566, 758
BmiI GGNNCC 3 cut(s) 154, 445, 567
BmrFI CCNGG 7 cut(s) 101, 321, 369, 387, 630, 973, 1161
BmrI ACTGGG 1 cut(s) 390
BmuI ACTGGG 1 cut(s) 390
BpmI CTGGAG 2 cut(s) 543, 1122
BpuEI CTTGAG 2 cut(s) 334, 760
BpuMI CCSGG 4 cut(s) 101, 321, 369, 1161
BsaAI YACGTR 2 cut(s) 346, 355
BsaHI GRCGYC 1 cut(s) 300
BsaI GGTCTC 3 cut(s) 128, 277, 962
BsaJI CCNNGG 5 cut(s) 99, 129, 244, 368, 1009
BsaXI ACNNNNNCTCC 4 cut(s) 516, 546, 1103, 1133
Bsc4I CCNNNNNNNGG 5 cut(s) 337, 368, 453, 826, 908
Bse118I RCCGGY 1 cut(s) 436
Bse1I ACTGG 6 cut(s) 219, 396, 458, 526, 736, 1065
Bse3DI GCAATG 1 cut(s) 786
BseBI CCWGG 3 cut(s) 387, 630, 973
BseDI CCNNGG 5 cut(s) 99, 129, 244, 368, 1009
BseGI GGATG 1 cut(s) 535
BseLI CCNNNNNNNGG 5 cut(s) 337, 368, 453, 826, 908
BseMI GCAATG 1 cut(s) 786
BseMII CTCAG 3 cut(s) 549, 696, 921
BseNI ACTGG 6 cut(s) 219, 396, 458, 526, 736, 1065
BseSI GKGCMC 1 cut(s) 916
BseXI GCAGC 4 cut(s) 233, 793, 944, 1010
BshFI GGCC 5 cut(s) 319, 324, 342, 436, 1132
BsiHKAI GWGCWC 1 cut(s) 916
BsiHKCI CYCGRG 1 cut(s) 570
BsiSI CCGG 6 cut(s) 101, 320, 368, 437, 447, 1160
BslFI GGGAC 6 cut(s) 82, 138, 384, 503, 532, 974
BslI CCNNNNNNNGG 5 cut(s) 337, 368, 453, 826, 908
BsmAI GTCTC 6 cut(s) 97, 128, 277, 751, 878, 962
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 6 cut(s) 82, 138, 384, 503, 532, 974
BsmI GAATGC 2 cut(s) 582, 1108
BsnI GGCC 5 cut(s) 319, 324, 342, 436, 1132
Bso31I GGTCTC 3 cut(s) 128, 277, 962
BsoBI CYCGRG 1 cut(s) 570
Bsp1286I GDGCHC 2 cut(s) 815, 916
Bsp143I GATC 3 cut(s) 443, 689, 1123
Bsp19I CCATGG 1 cut(s) 1009
BspANI GGCC 5 cut(s) 319, 324, 342, 436, 1132
BspCNI CTCAG 3 cut(s) 550, 697, 920
BspLI GGNNCC 3 cut(s) 154, 445, 567
BspMAI CTGCAG 1 cut(s) 934
BspMI ACCTGC 1 cut(s) 473
BspPI GGATC 3 cut(s) 438, 451, 697
BspTNI GGTCTC 3 cut(s) 128, 277, 962
BsrDI GCAATG 1 cut(s) 786
BsrFI RCCGGY 1 cut(s) 436
BsrI ACTGG 6 cut(s) 219, 396, 458, 526, 736, 1065
BssAI RCCGGY 1 cut(s) 436
BssECI CCNNGG 5 cut(s) 99, 129, 244, 368, 1009
BssMI GATC 3 cut(s) 443, 689, 1123
BssNI GRCGYC 1 cut(s) 300
BssT1I CCWWGG 2 cut(s) 244, 1009
Bst2UI CCWGG 3 cut(s) 387, 630, 973
Bst4CI ACNGT 4 cut(s) 514, 700, 892, 925
Bst6I CTCTTC 2 cut(s) 269, 421
BstACI GRCGYC 1 cut(s) 300
BstBAI YACGTR 2 cut(s) 346, 355
BstC8I GCNNGC 3 cut(s) 438, 811, 951
BstDEI CTNAG 3 cut(s) 558, 705, 907
BstDSI CCRYGG 1 cut(s) 1009
BstF5I GGATG 1 cut(s) 535
BstH2I RGCGCY 1 cut(s) 667
BstHHI GCGC 1 cut(s) 666
BstKTI GATC 3 cut(s) 446, 692, 1126
BstMAI GTCTC 6 cut(s) 97, 128, 277, 751, 878, 962
BstMBI GATC 3 cut(s) 443, 689, 1123
BstMWI GCNNNNNNNGC 3 cut(s) 36, 599, 732
BstNI CCWGG 3 cut(s) 387, 630, 973
BstNSI RCATGY 1 cut(s) 506
BstSCI CCNGG 7 cut(s) 99, 319, 367, 385, 628, 971, 1159
BstSFI CTRYAG 1 cut(s) 930
BstSLI GKGCMC 1 cut(s) 916
BstV1I GCAGC 4 cut(s) 233, 793, 944, 1010
BstX2I RGATCY 1 cut(s) 443
BstYI RGATCY 1 cut(s) 443
BsuRI GGCC 5 cut(s) 319, 324, 342, 436, 1132
BtgI CCRYGG 1 cut(s) 1009
BtsCI GGATG 1 cut(s) 535
BtsI GCAGTG 1 cut(s) 927
BtsIMutI CAGTG 4 cut(s) 702, 897, 927, 1072
BveI ACCTGC 1 cut(s) 473
Cac8I GCNNGC 3 cut(s) 438, 811, 951
CaiI CAGNNNCTG 1 cut(s) 704
CfoI GCGC 1 cut(s) 666
Cfr10I RCCGGY 1 cut(s) 436
Cfr13I GGNCC 5 cut(s) 152, 322, 340, 566, 758
CseI GACGC 1 cut(s) 308
CsiI ACCWGGT 2 cut(s) 628, 971
Csp6I GTAC 3 cut(s) 212, 295, 356
CspCI CAANNNNNGTGG 2 cut(s) 127, 162
CviAII CATG 4 cut(s) 503, 1010, 1030, 1081
CviQI GTAC 3 cut(s) 212, 295, 356
DdeI CTNAG 3 cut(s) 558, 705, 907
DpnI GATC 3 cut(s) 445, 691, 1125
DpnII GATC 3 cut(s) 443, 689, 1123
DrdI GACNNNNNNGTC 1 cut(s) 765
DseDI GACNNNNNNGTC 1 cut(s) 765
Eam1104I CTCTTC 2 cut(s) 269, 421
EarI CTCTTC 2 cut(s) 269, 421
Eco130I CCWWGG 2 cut(s) 244, 1009
Eco24I GRGCYC 1 cut(s) 815
Eco31I GGTCTC 3 cut(s) 128, 277, 962
Eco47I GGWCC 3 cut(s) 152, 566, 758
Eco57I CTGAAG 1 cut(s) 1136
Eco72I CACGTG 1 cut(s) 346
Eco88I CYCGRG 1 cut(s) 570
EcoO109I RGGNCCY 1 cut(s) 566
EcoRI GAATTC 1 cut(s) 543
EcoRII CCWGG 3 cut(s) 385, 628, 971
EcoT14I CCWWGG 2 cut(s) 244, 1009
EcoT38I GRGCYC 1 cut(s) 815
ErhI CCWWGG 2 cut(s) 244, 1009
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 4 cut(s) 506, 1013, 1033, 1084
FaiI YATR 9 cut(s) 504, 657, 797, 842, 1011, 1031, 1058, 1082, 1128
FaqI GGGAC 6 cut(s) 82, 138, 384, 503, 532, 974
FatI CATG 4 cut(s) 502, 1009, 1029, 1080
FauI CCCGC 1 cut(s) 529
Fnu4HI GCNGC 7 cut(s) 222, 362, 365, 736, 807, 933, 999
FokI GGATG 1 cut(s) 542
FriOI GRGCYC 1 cut(s) 815
Fsp4HI GCNGC 7 cut(s) 222, 362, 365, 736, 807, 933, 999
FspBI CTAG 1 cut(s) 143
GlaI GCGC 1 cut(s) 665
GluI GCNGC 7 cut(s) 222, 362, 365, 736, 807, 933, 999
GsuI CTGGAG 2 cut(s) 543, 1122
HaeII RGCGCY 1 cut(s) 667
HaeIII GGCC 5 cut(s) 319, 324, 342, 436, 1132
HapII CCGG 6 cut(s) 101, 320, 368, 437, 447, 1160
HgaI GACGC 1 cut(s) 308
HhaI GCGC 1 cut(s) 666
Hin1I GRCGYC 1 cut(s) 300
Hin1II CATG 4 cut(s) 506, 1013, 1033, 1084
Hin6I GCGC 1 cut(s) 664
HinP1I GCGC 1 cut(s) 664
HincII GTYRAC 3 cut(s) 109, 121, 256
HindII GTYRAC 3 cut(s) 109, 121, 256
HindIII AAGCTT 1 cut(s) 835
HinfI GANTC 3 cut(s) 53, 240, 349
HpaII CCGG 6 cut(s) 101, 320, 368, 437, 447, 1160
HphI GGTGA 1 cut(s) 48
Hpy166II GTNNAC 5 cut(s) 109, 121, 256, 696, 914
Hpy188I TCNGA 6 cut(s) 46, 133, 406, 559, 651, 1123
Hpy188III TCNNGA 3 cut(s) 687, 881, 1139
Hpy8I GTNNAC 5 cut(s) 109, 121, 256, 696, 914
Hpy99I CGWCG 2 cut(s) 302, 308
HpyAV CCTTC 4 cut(s) 415, 426, 451, 556
HpyCH4III ACNGT 4 cut(s) 514, 700, 892, 925
HpyCH4IV ACGT 3 cut(s) 123, 345, 354
HpyCH4V TGCA 6 cut(s) 221, 416, 582, 914, 932, 1106
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 599, 732
HpyF3I CTNAG 3 cut(s) 558, 705, 907
HpySE526I ACGT 3 cut(s) 123, 345, 354
Hsp92I GRCGYC 1 cut(s) 300
Hsp92II CATG 4 cut(s) 506, 1013, 1033, 1084
HspAI GCGC 1 cut(s) 664
KroI GCCGGC 1 cut(s) 436
KroNI GCCGGC 1 cut(s) 438
Kzo9I GATC 3 cut(s) 443, 689, 1123
LmnI GCTCC 2 cut(s) 599, 1111
Lsp1109I GCAGC 4 cut(s) 233, 793, 944, 1010
MabI ACCWGGT 2 cut(s) 628, 971
MaeI CTAG 1 cut(s) 143
MaeII ACGT 3 cut(s) 123, 345, 354
MaeIII GTNAC 5 cut(s) 124, 508, 613, 700, 925
MalI GATC 3 cut(s) 445, 691, 1125
MboI GATC 3 cut(s) 443, 689, 1123
MboII GAAGA 8 cut(s) 17, 280, 283, 286, 438, 627, 877, 880
MflI RGATCY 1 cut(s) 443
MhlI GDGCHC 2 cut(s) 815, 916
MluCI AATT 7 cut(s) 334, 543, 774, 822, 963, 1051, 1165
MlyI GAGTC 2 cut(s) 47, 249
MmeI TCCRAC 3 cut(s) 217, 811, 1079
MnlI CCTC 9 cut(s) 139, 272, 309, 412, 422, 520, 566, 579, 652
MroNI GCCGGC 1 cut(s) 436
MslI CAYNNNNRTG 2 cut(s) 879, 1079
MspI CCGG 6 cut(s) 101, 320, 368, 437, 447, 1160
MspR9I CCNGG 7 cut(s) 101, 321, 369, 387, 630, 973, 1161
Mva1269I GAATGC 2 cut(s) 582, 1108
MvaI CCWGG 3 cut(s) 387, 630, 973
MwoI GCNNNNNNNGC 3 cut(s) 36, 599, 732
NaeI GCCGGC 1 cut(s) 438
NciI CCSGG 4 cut(s) 101, 321, 369, 1161
NcoI CCATGG 1 cut(s) 1009
NdeII GATC 3 cut(s) 443, 689, 1123
NgoMIV GCCGGC 1 cut(s) 436
NlaIII CATG 4 cut(s) 506, 1013, 1033, 1084
NlaIV GGNNCC 3 cut(s) 154, 445, 567
NmeAIII GCCGAG 1 cut(s) 15
NmuCI GTSAC 3 cut(s) 124, 700, 925
NspI RCATGY 1 cut(s) 506
PaeR7I CTCGAG 1 cut(s) 570
PaqCI CACCTGC 1 cut(s) 473
PciI ACATGT 1 cut(s) 502
PcsI WCGNNNNNNNCGW 1 cut(s) 351
PctI GAATGC 2 cut(s) 582, 1108
PdiI GCCGGC 1 cut(s) 438
PfeI GAWTC 1 cut(s) 349
PflMI CCANNNNNTGG 1 cut(s) 826
PkrI GCNGC 7 cut(s) 223, 363, 366, 737, 808, 934, 1000
PleI GAGTC 2 cut(s) 47, 248
PmaCI CACGTG 1 cut(s) 346
PmlI CACGTG 1 cut(s) 346
PpsI GAGTC 2 cut(s) 47, 248
Ppu21I YACGTR 2 cut(s) 346, 355
PpuMI RGGWCCY 1 cut(s) 566
PscI ACATGT 1 cut(s) 502
Psp5II RGGWCCY 1 cut(s) 566
Psp6I CCWGG 3 cut(s) 385, 628, 971
PspCI CACGTG 1 cut(s) 346
PspGI CCWGG 3 cut(s) 385, 628, 971
PspN4I GGNNCC 3 cut(s) 154, 445, 567
PspPI GGNCC 5 cut(s) 152, 322, 340, 566, 758
PspPPI RGGWCCY 1 cut(s) 566
PspXI VCTCGAGB 1 cut(s) 570
PstI CTGCAG 1 cut(s) 934
PstNI CAGNNNCTG 1 cut(s) 704
PsuI RGATCY 1 cut(s) 443
RsaI GTAC 3 cut(s) 213, 296, 357
RsaNI GTAC 3 cut(s) 212, 295, 356
RseI CAYNNNNRTG 2 cut(s) 879, 1079
SatI GCNGC 7 cut(s) 222, 362, 365, 736, 807, 933, 999
Sau3AI GATC 3 cut(s) 443, 689, 1123
Sau96I GGNCC 5 cut(s) 152, 322, 340, 566, 758
ScaI AGTACT 1 cut(s) 213
SchI GAGTC 2 cut(s) 47, 249
ScrFI CCNGG 7 cut(s) 101, 321, 369, 387, 630, 973, 1161
SduI GDGCHC 2 cut(s) 815, 916
SexAI ACCWGGT 2 cut(s) 628, 971
SfcI CTRYAG 1 cut(s) 930
Sfr274I CTCGAG 1 cut(s) 570
SinI GGWCC 3 cut(s) 152, 566, 758
SlaI CTCGAG 1 cut(s) 570
SmiMI CAYNNNNRTG 2 cut(s) 879, 1079
SmlI CTYRAG 3 cut(s) 313, 570, 739
SmoI CTYRAG 3 cut(s) 313, 570, 739
SpeI ACTAGT 1 cut(s) 142
Sse9I AATT 7 cut(s) 334, 543, 774, 822, 963, 1051, 1165
SspMI CTAG 1 cut(s) 143
StyD4I CCNGG 7 cut(s) 99, 319, 367, 385, 628, 971, 1159
StyI CCWWGG 2 cut(s) 244, 1009
TaaI ACNGT 4 cut(s) 514, 700, 892, 925
TaiI ACGT 3 cut(s) 126, 348, 357
TaqI TCGA 1 cut(s) 571
TasI AATT 7 cut(s) 334, 543, 774, 822, 963, 1051, 1165
TatI WGTACW 1 cut(s) 211
TauI GCSGC 3 cut(s) 364, 367, 738
TfiI GAWTC 1 cut(s) 349
TscAI CASTG 4 cut(s) 709, 897, 934, 1072
TseFI GTSAC 3 cut(s) 124, 700, 925
TseI GCWGC 4 cut(s) 221, 806, 932, 998
Tsp45I GTSAC 3 cut(s) 124, 700, 925
TspDTI ATGAA 1 cut(s) 1045
TspGWI ACGGA 2 cut(s) 321, 1124
TspRI CASTG 4 cut(s) 709, 897, 934, 1072
Van91I CCANNNNNTGG 1 cut(s) 826
VneI GTGCAC 1 cut(s) 912
VpaK11BI GGWCC 3 cut(s) 152, 566, 758
XapI RAATTY 1 cut(s) 543
XceI RCATGY 1 cut(s) 506
XcmI CCANNNNNNNNNTGG 1 cut(s) 827
XhoI CTCGAG 1 cut(s) 570
XspI CTAG 1 cut(s) 143
ZrmI AGTACT 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.