RLG00000024122

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0012637
Physical Location & Seq
Reverse (-)
32161068 .. 32163499
2432 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024122

Sequence Viewer

Length: 1155 bp
ATGGAAGAAAAGGAAAGCACCTTTTCTGGCTCACTCGGCAACTCGGATACCGACTCACCCCCACCGAACTCCTTCAACCACACCGCCGTCTCGTCCCCGGTGGTCAACACGGCGGTCAACGTGACCTCAGAGACCACACTGGTTGGTCCCACAGCACCAGCACCACTGCCGCAACCAACAGCACCAACATCACAAGTACTGGTTGCAGCAAACACAGGTGTTGGAGTCCAAGGAAATGTTGACTTGTCTGGGAAGAAGAAGAGAGGGAGACCCAGAAAGTACGACGCCAACGGAAACTTGAGGCCGGGCCAGACCCACAATTCTGGTCACGTGATTCACGTGCCGCCGCCGCCGGGGTTTTATCTCTCGCCTGGTTCTCCCAGTCCCTCCGAGTTTTCTGCAAAGAAGGGAAGAGGAAGGCCGGCTGGATCCGGCAACTGGCAGGTTCTTGCTTCTTTAGAAACAGCAGGTGGGGACTTCACACCACATGTGGTTACTGTTGGGACTGGAGAGGATGTTGCGGGGAGAATTCTGTCATTTTCTGAGAAGGGTCCTCGAGGGATTTGCATTCTCTCTGCTAATGGAGCTGTTTCAAATGTCACCATTCGCCAACCTGGTTCTTCAGGCGGTATTCTGACTTATGAGGGCCGCTTTGAGTTGTTGTCTTTATCGGGGTCGTTTACAGTCACTGAGATTGGTGGTGTAAGAAGCAGGACTGGCGGCTTGAGTGTCTCATTGGCCGGACCAGATGGTCGTGTAATTGGTGGCGGCATTGCTGGTATGCTAACAGCTGCGAGCCCTATTCAAATTGTGGTTGGAAGCTTTATGCCGAATGGGTGTAAGCCACAGAAGAAGAAGCATTATCGTGAGACAACAGTAGCTTCTCCAATCTCAGGTGCACCAGATACAGTCACCGCAGCAACACCTATCTCGCAAGCACAACCTGAAATCGAGACCAGGTTTACCCCAATAGTCCCTTTGGCAGCACAAATCCATGGAGAAGCAGAAAAAGGCATGAGCCAAAACCAGAACTTCAATTTCATACCCACCAGTGCTTCCATTCTCATGCCTACTAATGCTGGTTGGAATGCAACGGAGCTGAAGTCAGATCATAGGCCATCTCCAGACATTAATGTATCTGTTCCGGGTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook DUF296

Protein Analysis

385

Amino Acids

39.47

Weight (kDa)

8.85

Isoelectric Point (pI)

43.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 161 - 275 1.2e-23 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 458
AasI GACNNNNNNGTC 1 cut(s) 750
AbsI CCTCGAGG 1 cut(s) 555
Acc36I ACCTGC 2 cut(s) 433, 458
AclWI GGATC 2 cut(s) 423, 436
AcoI YGGCCR 1 cut(s) 738
AcsI RAATTY 1 cut(s) 528
AcuI CTGAAG 2 cut(s) 606, 1121
AcvI CACGTG 2 cut(s) 331, 340
AcyI GRCGYC 1 cut(s) 285
AfaI GTAC 2 cut(s) 198, 281
AfiI CCNNNNNNNGG 3 cut(s) 353, 438, 893
AflIII ACRYGT 1 cut(s) 487
AgsI TTSAA 4 cut(s) 76, 594, 806, 1036
AjnI CCWGG 3 cut(s) 370, 613, 956
AluBI AGCT 5 cut(s) 587, 791, 822, 881, 1099
AluI AGCT 5 cut(s) 587, 791, 822, 881, 1099
Alw21I GWGCWC 1 cut(s) 901
Alw26I GTCTC 6 cut(s) 94, 125, 262, 736, 863, 947
Alw44I GTGCAC 1 cut(s) 897
AlwI GGATC 2 cut(s) 423, 436
AlwNI CAGNNNCTG 1 cut(s) 689
Ama87I CYCGRG 1 cut(s) 555
AoxI GGCC 6 cut(s) 302, 307, 419, 646, 738, 1115
ApaLI GTGCAC 1 cut(s) 897
ApeKI GCWGC 4 cut(s) 206, 791, 917, 983
ApoI RAATTY 1 cut(s) 528
AseI ATTAAT 1 cut(s) 1131
Asp700I GAANNNNTTC 1 cut(s) 71
AspS9I GGNCC 5 cut(s) 146, 307, 551, 646, 743
AsuC2I CCSGG 4 cut(s) 98, 306, 354, 1146
AsuHPI GGTGA 3 cut(s) 48, 592, 904
AvaI CYCGRG 1 cut(s) 555
AvaII GGWCC 3 cut(s) 146, 551, 743
BaeGI GKGCMC 1 cut(s) 901
BamHI GGATCC 1 cut(s) 428
BanII GRGCYC 1 cut(s) 800
BbrPI CACGTG 2 cut(s) 331, 340
Bbv12I GWGCWC 1 cut(s) 901
BbvI GCAGC 4 cut(s) 218, 778, 929, 995
BccI CCATC 2 cut(s) 743, 1126
BceAI ACGGC 2 cut(s) 71, 126
BcgI CGANNNNNNTGC 4 cut(s) 380, 414, 546, 580
BciT130I CCWGG 3 cut(s) 372, 615, 958
BciVI GTATCC 1 cut(s) 40
BcnI CCSGG 4 cut(s) 98, 306, 354, 1146
BcoDI GTCTC 6 cut(s) 94, 125, 262, 736, 863, 947
BfuAI ACCTGC 2 cut(s) 433, 458
BfuI GTATCC 1 cut(s) 40
BmcAI AGTACT 1 cut(s) 198
Bme1390I CCNGG 7 cut(s) 98, 306, 354, 372, 615, 958, 1146
Bme18I GGWCC 3 cut(s) 146, 551, 743
BmeT110I CYCGRG 1 cut(s) 555
BmgT120I GGNCC 5 cut(s) 146, 307, 551, 646, 743
BmiI GGNNCC 3 cut(s) 148, 430, 552
BmrFI CCNGG 7 cut(s) 98, 306, 354, 372, 615, 958, 1146
BmrI ACTGGG 1 cut(s) 375
BmuI ACTGGG 1 cut(s) 375
BpmI CTGGAG 2 cut(s) 528, 1107
BpuEI CTTGAG 2 cut(s) 319, 745
BpuMI CCSGG 4 cut(s) 98, 306, 354, 1146
BsaAI YACGTR 2 cut(s) 331, 340
BsaHI GRCGYC 1 cut(s) 285
BsaI GGTCTC 3 cut(s) 125, 262, 947
BsaJI CCNNGG 4 cut(s) 96, 229, 353, 994
BsaXI ACNNNNNCTCC 4 cut(s) 501, 531, 1088, 1118
Bsc4I CCNNNNNNNGG 3 cut(s) 353, 438, 893
Bse118I RCCGGY 1 cut(s) 421
Bse1I ACTGG 7 cut(s) 144, 204, 381, 443, 511, 721, 1050
Bse3DI GCAATG 1 cut(s) 771
BseBI CCWGG 3 cut(s) 372, 615, 958
BseDI CCNNGG 4 cut(s) 96, 229, 353, 994
BseGI GGATG 1 cut(s) 520
BseLI CCNNNNNNNGG 3 cut(s) 353, 438, 893
BseMI GCAATG 1 cut(s) 771
BseMII CTCAG 4 cut(s) 141, 534, 681, 906
BseNI ACTGG 7 cut(s) 144, 204, 381, 443, 511, 721, 1050
BseSI GKGCMC 1 cut(s) 901
BseXI GCAGC 4 cut(s) 218, 778, 929, 995
BshFI GGCC 6 cut(s) 304, 309, 421, 648, 740, 1117
BsiHKAI GWGCWC 1 cut(s) 901
BsiHKCI CYCGRG 1 cut(s) 555
BsiSI CCGG 7 cut(s) 98, 305, 353, 422, 432, 741, 1145
BslFI GGGAC 6 cut(s) 79, 132, 369, 488, 517, 959
BslI CCNNNNNNNGG 3 cut(s) 353, 438, 893
BsmAI GTCTC 6 cut(s) 94, 125, 262, 736, 863, 947
BsmBI CGTCTC 1 cut(s) 94
BsmFI GGGAC 6 cut(s) 79, 132, 369, 488, 517, 959
BsmI GAATGC 2 cut(s) 567, 1093
BsnI GGCC 6 cut(s) 304, 309, 421, 648, 740, 1117
Bso31I GGTCTC 3 cut(s) 125, 262, 947
BsoBI CYCGRG 1 cut(s) 555
Bsp1286I GDGCHC 2 cut(s) 800, 901
Bsp143I GATC 2 cut(s) 428, 1108
Bsp19I CCATGG 1 cut(s) 994
BspANI GGCC 6 cut(s) 304, 309, 421, 648, 740, 1117
BspCNI CTCAG 4 cut(s) 140, 535, 682, 905
BspLI GGNNCC 3 cut(s) 148, 430, 552
BspMI ACCTGC 2 cut(s) 433, 458
BspPI GGATC 2 cut(s) 423, 436
BspTNI GGTCTC 3 cut(s) 125, 262, 947
BsrDI GCAATG 1 cut(s) 771
BsrFI RCCGGY 1 cut(s) 421
BsrI ACTGG 7 cut(s) 144, 204, 381, 443, 511, 721, 1050
BssAI RCCGGY 1 cut(s) 421
BssECI CCNNGG 4 cut(s) 96, 229, 353, 994
BssMI GATC 2 cut(s) 428, 1108
BssNI GRCGYC 1 cut(s) 285
BssT1I CCWWGG 2 cut(s) 229, 994
Bst2UI CCWGG 3 cut(s) 372, 615, 958
Bst4CI ACNGT 4 cut(s) 499, 685, 877, 910
Bst6I CTCTTC 2 cut(s) 254, 406
BstACI GRCGYC 1 cut(s) 285
BstBAI YACGTR 2 cut(s) 331, 340
BstC8I GCNNGC 3 cut(s) 423, 796, 936
BstDEI CTNAG 4 cut(s) 127, 543, 690, 892
BstDSI CCRYGG 1 cut(s) 994
BstF5I GGATG 1 cut(s) 520
BstKTI GATC 2 cut(s) 431, 1111
BstMAI GTCTC 6 cut(s) 94, 125, 262, 736, 863, 947
BstMBI GATC 2 cut(s) 428, 1108
BstMWI GCNNNNNNNGC 4 cut(s) 36, 349, 584, 717
BstNI CCWGG 3 cut(s) 372, 615, 958
BstNSI RCATGY 1 cut(s) 491
BstSCI CCNGG 7 cut(s) 96, 304, 352, 370, 613, 956, 1144
BstSLI GKGCMC 1 cut(s) 901
BstV1I GCAGC 4 cut(s) 218, 778, 929, 995
BstX2I RGATCY 1 cut(s) 428
BstXI CCANNNNNNTGG 1 cut(s) 323
BstYI RGATCY 1 cut(s) 428
BsuI GTATCC 1 cut(s) 40
BsuRI GGCC 6 cut(s) 304, 309, 421, 648, 740, 1117
BtgI CCRYGG 1 cut(s) 994
BtsCI GGATG 1 cut(s) 520
BtsI GCAGTG 1 cut(s) 164
BtsIMutI CAGTG 4 cut(s) 137, 164, 687, 1057
BveI ACCTGC 2 cut(s) 433, 458
Cac8I GCNNGC 3 cut(s) 423, 796, 936
CaiI CAGNNNCTG 1 cut(s) 689
Cfr10I RCCGGY 1 cut(s) 421
Cfr13I GGNCC 5 cut(s) 146, 307, 551, 646, 743
CseI GACGC 1 cut(s) 293
CsiI ACCWGGT 2 cut(s) 613, 956
Csp6I GTAC 2 cut(s) 197, 280
CspCI CAANNNNNGTGG 2 cut(s) 124, 159
CviAII CATG 4 cut(s) 488, 995, 1015, 1066
CviQI GTAC 2 cut(s) 197, 280
DdeI CTNAG 4 cut(s) 127, 543, 690, 892
DpnI GATC 2 cut(s) 430, 1110
DpnII GATC 2 cut(s) 428, 1108
DrdI GACNNNNNNGTC 1 cut(s) 750
DseDI GACNNNNNNGTC 1 cut(s) 750
EaeI YGGCCR 1 cut(s) 738
Eam1104I CTCTTC 2 cut(s) 254, 406
EarI CTCTTC 2 cut(s) 254, 406
Eco130I CCWWGG 2 cut(s) 229, 994
Eco24I GRGCYC 1 cut(s) 800
Eco31I GGTCTC 3 cut(s) 125, 262, 947
Eco47I GGWCC 3 cut(s) 146, 551, 743
Eco57I CTGAAG 2 cut(s) 606, 1121
Eco72I CACGTG 2 cut(s) 331, 340
Eco88I CYCGRG 1 cut(s) 555
EcoO109I RGGNCCY 1 cut(s) 551
EcoRI GAATTC 1 cut(s) 528
EcoRII CCWGG 3 cut(s) 370, 613, 956
EcoT14I CCWWGG 2 cut(s) 229, 994
EcoT38I GRGCYC 1 cut(s) 800
ErhI CCWWGG 2 cut(s) 229, 994
Esp3I CGTCTC 1 cut(s) 94
FaeI CATG 4 cut(s) 491, 998, 1018, 1069
FaiI YATR 9 cut(s) 489, 642, 782, 827, 996, 1016, 1043, 1067, 1113
FaqI GGGAC 6 cut(s) 79, 132, 369, 488, 517, 959
FatI CATG 4 cut(s) 487, 994, 1014, 1065
FauI CCCGC 1 cut(s) 514
FokI GGATG 1 cut(s) 527
FriOI GRGCYC 1 cut(s) 800
GsuI CTGGAG 2 cut(s) 528, 1107
HaeIII GGCC 6 cut(s) 304, 309, 421, 648, 740, 1117
HapII CCGG 7 cut(s) 98, 305, 353, 422, 432, 741, 1145
HgaI GACGC 1 cut(s) 293
Hin1I GRCGYC 1 cut(s) 285
Hin1II CATG 4 cut(s) 491, 998, 1018, 1069
HincII GTYRAC 3 cut(s) 106, 118, 241
HindII GTYRAC 3 cut(s) 106, 118, 241
HindIII AAGCTT 1 cut(s) 820
HinfI GANTC 3 cut(s) 53, 225, 334
HpaII CCGG 7 cut(s) 98, 305, 353, 422, 432, 741, 1145
HphI GGTGA 3 cut(s) 48, 592, 904
Hpy166II GTNNAC 6 cut(s) 106, 118, 241, 681, 899, 963
Hpy188I TCNGA 6 cut(s) 46, 130, 391, 544, 636, 1108
Hpy188III TCNNGA 3 cut(s) 866, 952, 1124
Hpy8I GTNNAC 6 cut(s) 106, 118, 241, 681, 899, 963
Hpy99I CGWCG 1 cut(s) 287
HpyAV CCTTC 4 cut(s) 82, 400, 411, 541
HpyCH4III ACNGT 4 cut(s) 499, 685, 877, 910
HpyCH4IV ACGT 3 cut(s) 120, 330, 339
HpyCH4V TGCA 5 cut(s) 206, 401, 567, 899, 1091
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 349, 584, 717
HpyF3I CTNAG 4 cut(s) 127, 543, 690, 892
HpySE526I ACGT 3 cut(s) 120, 330, 339
Hsp92I GRCGYC 1 cut(s) 285
Hsp92II CATG 4 cut(s) 491, 998, 1018, 1069
KroI GCCGGC 1 cut(s) 421
KroNI GCCGGC 1 cut(s) 423
Kzo9I GATC 2 cut(s) 428, 1108
LmnI GCTCC 2 cut(s) 584, 1096
Lsp1109I GCAGC 4 cut(s) 218, 778, 929, 995
MabI ACCWGGT 2 cut(s) 613, 956
MaeII ACGT 3 cut(s) 120, 330, 339
MaeIII GTNAC 6 cut(s) 121, 326, 493, 598, 685, 910
MalI GATC 2 cut(s) 430, 1110
MboI GATC 2 cut(s) 428, 1108
MboII GAAGA 8 cut(s) 17, 265, 268, 271, 423, 612, 862, 865
MflI RGATCY 1 cut(s) 428
MhlI GDGCHC 2 cut(s) 800, 901
MluCI AATT 6 cut(s) 319, 528, 759, 807, 1036, 1150
MlyI GAGTC 2 cut(s) 47, 234
MmeI TCCRAC 3 cut(s) 202, 796, 1064
MnlI CCTC 9 cut(s) 136, 257, 294, 397, 407, 505, 551, 564, 637
MroNI GCCGGC 1 cut(s) 421
MroXI GAANNNNTTC 1 cut(s) 71
MseI TTAA 1 cut(s) 1131
MslI CAYNNNNRTG 2 cut(s) 864, 1064
MspA1I CMGCKG 1 cut(s) 791
MspI CCGG 7 cut(s) 98, 305, 353, 422, 432, 741, 1145
MspR9I CCNGG 7 cut(s) 98, 306, 354, 372, 615, 958, 1146
Mva1269I GAATGC 2 cut(s) 567, 1093
MvaI CCWGG 3 cut(s) 372, 615, 958
MwoI GCNNNNNNNGC 4 cut(s) 36, 349, 584, 717
NaeI GCCGGC 1 cut(s) 423
NciI CCSGG 4 cut(s) 98, 306, 354, 1146
NcoI CCATGG 1 cut(s) 994
NdeII GATC 2 cut(s) 428, 1108
NgoMIV GCCGGC 1 cut(s) 421
NlaIII CATG 4 cut(s) 491, 998, 1018, 1069
NlaIV GGNNCC 3 cut(s) 148, 430, 552
NmeAIII GCCGAG 1 cut(s) 15
NmuCI GTSAC 5 cut(s) 121, 326, 598, 685, 910
NspI RCATGY 1 cut(s) 491
PaeR7I CTCGAG 1 cut(s) 555
PaqCI CACCTGC 1 cut(s) 458
PciI ACATGT 1 cut(s) 487
PcsI WCGNNNNNNNCGW 1 cut(s) 336
PctI GAATGC 2 cut(s) 567, 1093
PdiI GCCGGC 1 cut(s) 423
PdmI GAANNNNTTC 1 cut(s) 71
PfeI GAWTC 1 cut(s) 334
PleI GAGTC 2 cut(s) 47, 233
PmaCI CACGTG 2 cut(s) 331, 340
PmlI CACGTG 2 cut(s) 331, 340
PpsI GAGTC 2 cut(s) 47, 233
Ppu21I YACGTR 2 cut(s) 331, 340
PpuMI RGGWCCY 1 cut(s) 551
PscI ACATGT 1 cut(s) 487
PshBI ATTAAT 1 cut(s) 1131
Psp5II RGGWCCY 1 cut(s) 551
Psp6I CCWGG 3 cut(s) 370, 613, 956
PspCI CACGTG 2 cut(s) 331, 340
PspGI CCWGG 3 cut(s) 370, 613, 956
PspN4I GGNNCC 3 cut(s) 148, 430, 552
PspPI GGNCC 5 cut(s) 146, 307, 551, 646, 743
PspPPI RGGWCCY 1 cut(s) 551
PspXI VCTCGAGB 1 cut(s) 555
PstNI CAGNNNCTG 1 cut(s) 689
PsuI RGATCY 1 cut(s) 428
PvuII CAGCTG 1 cut(s) 791
RsaI GTAC 2 cut(s) 198, 281
RsaNI GTAC 2 cut(s) 197, 280
RseI CAYNNNNRTG 2 cut(s) 864, 1064
SaqAI TTAA 1 cut(s) 1131
Sau3AI GATC 2 cut(s) 428, 1108
Sau96I GGNCC 5 cut(s) 146, 307, 551, 646, 743
ScaI AGTACT 1 cut(s) 198
SchI GAGTC 2 cut(s) 47, 234
ScrFI CCNGG 7 cut(s) 98, 306, 354, 372, 615, 958, 1146
SduI GDGCHC 2 cut(s) 800, 901
SexAI ACCWGGT 2 cut(s) 613, 956
Sfr274I CTCGAG 1 cut(s) 555
SinI GGWCC 3 cut(s) 146, 551, 743
SlaI CTCGAG 1 cut(s) 555
SmiMI CAYNNNNRTG 2 cut(s) 864, 1064
SmlI CTYRAG 3 cut(s) 298, 555, 724
SmoI CTYRAG 3 cut(s) 298, 555, 724
Sse9I AATT 6 cut(s) 319, 528, 759, 807, 1036, 1150
StyD4I CCNGG 7 cut(s) 96, 304, 352, 370, 613, 956, 1144
StyI CCWWGG 2 cut(s) 229, 994
TaaI ACNGT 4 cut(s) 499, 685, 877, 910
TaiI ACGT 3 cut(s) 123, 333, 342
TaqI TCGA 2 cut(s) 556, 951
TasI AATT 6 cut(s) 319, 528, 759, 807, 1036, 1150
TatI WGTACW 1 cut(s) 196
TauI GCSGC 7 cut(s) 172, 346, 349, 352, 651, 723, 771
TfiI GAWTC 1 cut(s) 334
Tru1I TTAA 1 cut(s) 1131
Tru9I TTAA 1 cut(s) 1131
TscAI CASTG 4 cut(s) 144, 171, 694, 1057
TseFI GTSAC 5 cut(s) 121, 326, 598, 685, 910
TseI GCWGC 4 cut(s) 206, 791, 917, 983
Tsp45I GTSAC 5 cut(s) 121, 326, 598, 685, 910
TspDTI ATGAA 1 cut(s) 1030
TspGWI ACGGA 2 cut(s) 306, 1109
TspRI CASTG 4 cut(s) 144, 171, 694, 1057
VneI GTGCAC 1 cut(s) 897
VpaK11BI GGWCC 3 cut(s) 146, 551, 743
VspI ATTAAT 1 cut(s) 1131
XapI RAATTY 1 cut(s) 528
XceI RCATGY 1 cut(s) 491
XhoI CTCGAG 1 cut(s) 555
XmnI GAANNNNTTC 1 cut(s) 71
ZrmI AGTACT 1 cut(s) 198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.