Rh3CG188700

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0012637
Physical Location & Seq
Forward (+)
16605678 .. 16608751
3074 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG188700.1

Sequence Viewer

Length: 843 bp
ATGGAAGAAAAGGAAAGCACCTTTTCTGGCTCACTCGGCAACTCGGACACCGACTCACCCCCACCCAATAACTCGTTCAACCACACCGCCGTCTCGTCCCCGGTGGTCAACACGGCGGTCAACGTGACCTCAGAGACCACACTAGTTGTTGGTCCCACAGCACCAACAGCACCACCTCAACAAACAGCACCAACATCACAAGTACTGGTTGCAGCAAACACAGGTGTTAGAGTCCAAGGAAATGTTGACTTGCCTGGGAAGAAGAAGAGAGGGAGACCCAGAAAGTACGACGCCGACGGAAACTTGAGGCCGGGCCAGACCCACAATTCGGGCCACGTGATTCACGTGCCGCCGCCGCCGGGGTTTTATCTCTCGCCTGGTTCTCCCAGTCCCTCCGAGTTTTCTGCAAAGAAGGGAAGAGGAAGGCCGGCTGGATCCGGCAACTGGCAGGTTCTTGCTTCTTTAGAAACAGCAGGTGGGGACTTCACACCACATGTGGTTACTGTTGGGACCGGAGAGGATGTTGCGGGGAGAATTCTGTCATTTTCTGAGAAGGGTCCTCGAGGGATTTGCATTCTCTCTGCTAATGGAGCTGTGTCAAATGTTACCATTCGCCAACCTGGTTCTTCTGGCGGTATTCTGACTTATGAGGGGCGCTTTGAGTTGTTGTCTTTATCGGGATCGTTTACAGTCACTGAGGTTGGTGGTGTAAGAAGCAGGACTGGCGGCTTGAGTGTCTCATTGGCTGGACCAGATGGTCGTGTAATTGGTGGTGGCATTGCTGGTATGCTAACAGCTGCGAGCCCTATCCAAGTAAGGAGTTTTTTACACATTTCCATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AT_hook DUF296

Protein Analysis

280

Amino Acids

28.58

Weight (kDa)

9.41

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 163 - 272 2.1e-21 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 464
AasI GACNNNNNNGTC 1 cut(s) 756
AbsI CCTCGAGG 1 cut(s) 561
Acc36I ACCTGC 2 cut(s) 439, 464
AciI CCGC 8 cut(s) 87, 116, 350, 353, 356, 527, 633, 726
AclWI GGATC 3 cut(s) 429, 442, 688
AcsI RAATTY 1 cut(s) 534
AcvI CACGTG 2 cut(s) 337, 346
AcyI GRCGYC 1 cut(s) 291
AfaI GTAC 2 cut(s) 204, 287
AfiI CCNNNNNNNGG 3 cut(s) 328, 359, 444
AflIII ACRYGT 1 cut(s) 493
AgsI TTSAA 1 cut(s) 79
AhlI ACTAGT 1 cut(s) 142
AjnI CCWGG 3 cut(s) 253, 376, 619
AjuI GAANNNNNNNTTGG 2 cut(s) 59, 91
AluBI AGCT 2 cut(s) 593, 797
AluI AGCT 2 cut(s) 593, 797
Alw26I GTCTC 4 cut(s) 97, 128, 268, 742
AlwI GGATC 3 cut(s) 429, 442, 688
AlwNI CAGNNNCTG 1 cut(s) 695
Ama87I CYCGRG 1 cut(s) 561
AoxI GGCC 4 cut(s) 308, 313, 331, 425
ApeKI GCWGC 2 cut(s) 212, 797
ApoI RAATTY 1 cut(s) 534
ArsI GACNNNNNNTTYG 2 cut(s) 310, 342
AspLEI GCGC 1 cut(s) 657
AspS9I GGNCC 6 cut(s) 152, 313, 331, 510, 557, 749
AsuC2I CCSGG 3 cut(s) 101, 312, 360
AsuHPI GGTGA 1 cut(s) 48
AvaI CYCGRG 1 cut(s) 561
AvaII GGWCC 4 cut(s) 152, 510, 557, 749
BamHI GGATCC 1 cut(s) 434
BanII GRGCYC 1 cut(s) 806
BbrPI CACGTG 2 cut(s) 337, 346
BbvI GCAGC 2 cut(s) 224, 784
BccI CCATC 1 cut(s) 749
BceAI ACGGC 2 cut(s) 74, 129
BcgI CGANNNNNNTGC 4 cut(s) 386, 420, 552, 586
BciT130I CCWGG 3 cut(s) 255, 378, 621
BcnI CCSGG 3 cut(s) 101, 312, 360
BcoDI GTCTC 4 cut(s) 97, 128, 268, 742
BcuI ACTAGT 1 cut(s) 142
BfaI CTAG 1 cut(s) 143
BfoI RGCGCY 1 cut(s) 658
BfuAI ACCTGC 2 cut(s) 439, 464
BisI GCNGC 6 cut(s) 213, 350, 353, 356, 727, 798
BlsI GCNGC 6 cut(s) 214, 351, 354, 357, 728, 799
BmcAI AGTACT 1 cut(s) 204
Bme1390I CCNGG 6 cut(s) 101, 255, 312, 360, 378, 621
Bme18I GGWCC 4 cut(s) 152, 510, 557, 749
BmeT110I CYCGRG 1 cut(s) 561
BmgT120I GGNCC 6 cut(s) 152, 313, 331, 510, 557, 749
BmiI GGNNCC 4 cut(s) 154, 436, 511, 558
BmrFI CCNGG 6 cut(s) 101, 255, 312, 360, 378, 621
BmrI ACTGGG 1 cut(s) 381
BmuI ACTGGG 1 cut(s) 381
BpuEI CTTGAG 2 cut(s) 325, 751
BpuMI CCSGG 3 cut(s) 101, 312, 360
BsaAI YACGTR 2 cut(s) 337, 346
BsaHI GRCGYC 1 cut(s) 291
BsaI GGTCTC 2 cut(s) 128, 268
BsaJI CCNNGG 4 cut(s) 99, 235, 254, 359
BsaWI WCCGGW 1 cut(s) 512
BsaXI ACNNNNNCTCC 2 cut(s) 507, 537
Bsc4I CCNNNNNNNGG 3 cut(s) 328, 359, 444
Bse118I RCCGGY 1 cut(s) 427
Bse1I ACTGG 4 cut(s) 210, 387, 449, 727
Bse3DI GCAATG 1 cut(s) 777
BseBI CCWGG 3 cut(s) 255, 378, 621
BseDI CCNNGG 4 cut(s) 99, 235, 254, 359
BseGI GGATG 1 cut(s) 526
BseLI CCNNNNNNNGG 3 cut(s) 328, 359, 444
BseMI GCAATG 1 cut(s) 777
BseMII CTCAG 3 cut(s) 144, 540, 687
BseNI ACTGG 4 cut(s) 210, 387, 449, 727
BseXI GCAGC 2 cut(s) 224, 784
BshFI GGCC 4 cut(s) 310, 315, 333, 427
BsiHKCI CYCGRG 1 cut(s) 561
BsiSI CCGG 6 cut(s) 101, 311, 359, 428, 438, 513
BslFI GGGAC 5 cut(s) 82, 138, 375, 494, 523
BslI CCNNNNNNNGG 3 cut(s) 328, 359, 444
BsmAI GTCTC 4 cut(s) 97, 128, 268, 742
BsmBI CGTCTC 1 cut(s) 97
BsmFI GGGAC 5 cut(s) 82, 138, 375, 494, 523
BsmI GAATGC 1 cut(s) 573
BsnI GGCC 4 cut(s) 310, 315, 333, 427
Bso31I GGTCTC 2 cut(s) 128, 268
BsoBI CYCGRG 1 cut(s) 561
Bsp1286I GDGCHC 1 cut(s) 806
Bsp143I GATC 2 cut(s) 434, 680
BspACI CCGC 8 cut(s) 87, 116, 350, 353, 356, 527, 633, 726
BspANI GGCC 4 cut(s) 310, 315, 333, 427
BspCNI CTCAG 3 cut(s) 143, 541, 688
BspLI GGNNCC 4 cut(s) 154, 436, 511, 558
BspMI ACCTGC 2 cut(s) 439, 464
BspPI GGATC 3 cut(s) 429, 442, 688
BspTNI GGTCTC 2 cut(s) 128, 268
BsrDI GCAATG 1 cut(s) 777
BsrFI RCCGGY 1 cut(s) 427
BsrI ACTGG 4 cut(s) 210, 387, 449, 727
BssAI RCCGGY 1 cut(s) 427
BssECI CCNNGG 4 cut(s) 99, 235, 254, 359
BssMI GATC 2 cut(s) 434, 680
BssNI GRCGYC 1 cut(s) 291
BssT1I CCWWGG 1 cut(s) 235
Bst2UI CCWGG 3 cut(s) 255, 378, 621
Bst4CI ACNGT 2 cut(s) 505, 691
Bst6I CTCTTC 2 cut(s) 260, 412
BstACI GRCGYC 1 cut(s) 291
BstBAI YACGTR 2 cut(s) 337, 346
BstC8I GCNNGC 2 cut(s) 429, 802
BstDEI CTNAG 3 cut(s) 130, 549, 696
BstF5I GGATG 1 cut(s) 526
BstH2I RGCGCY 1 cut(s) 658
BstHHI GCGC 1 cut(s) 657
BstKTI GATC 2 cut(s) 437, 683
BstMAI GTCTC 4 cut(s) 97, 128, 268, 742
BstMBI GATC 2 cut(s) 434, 680
BstMWI GCNNNNNNNGC 5 cut(s) 36, 167, 355, 590, 723
BstNI CCWGG 3 cut(s) 255, 378, 621
BstNSI RCATGY 1 cut(s) 497
BstSCI CCNGG 6 cut(s) 99, 253, 310, 358, 376, 619
BstV1I GCAGC 2 cut(s) 224, 784
BstX2I RGATCY 1 cut(s) 434
BstYI RGATCY 1 cut(s) 434
BsuRI GGCC 4 cut(s) 310, 315, 333, 427
BtsCI GGATG 1 cut(s) 526
BtsIMutI CAGTG 1 cut(s) 693
BveI ACCTGC 2 cut(s) 439, 464
Cac8I GCNNGC 2 cut(s) 429, 802
CaiI CAGNNNCTG 1 cut(s) 695
CfoI GCGC 1 cut(s) 657
Cfr10I RCCGGY 1 cut(s) 427
Cfr13I GGNCC 6 cut(s) 152, 313, 331, 510, 557, 749
CseI GACGC 1 cut(s) 299
CsiI ACCWGGT 1 cut(s) 619
Csp6I GTAC 2 cut(s) 203, 286
CspCI CAANNNNNGTGG 2 cut(s) 127, 162
CviAII CATG 1 cut(s) 494
CviQI GTAC 2 cut(s) 203, 286
DdeI CTNAG 3 cut(s) 130, 549, 696
DpnI GATC 2 cut(s) 436, 682
DpnII GATC 2 cut(s) 434, 680
DrdI GACNNNNNNGTC 1 cut(s) 756
DseDI GACNNNNNNGTC 1 cut(s) 756
Eam1104I CTCTTC 2 cut(s) 260, 412
EarI CTCTTC 2 cut(s) 260, 412
Eco130I CCWWGG 1 cut(s) 235
Eco24I GRGCYC 1 cut(s) 806
Eco31I GGTCTC 2 cut(s) 128, 268
Eco47I GGWCC 4 cut(s) 152, 510, 557, 749
Eco72I CACGTG 2 cut(s) 337, 346
Eco88I CYCGRG 1 cut(s) 561
EcoO109I RGGNCCY 1 cut(s) 557
EcoRI GAATTC 1 cut(s) 534
EcoRII CCWGG 3 cut(s) 253, 376, 619
EcoT14I CCWWGG 1 cut(s) 235
EcoT38I GRGCYC 1 cut(s) 806
ErhI CCWWGG 1 cut(s) 235
Esp3I CGTCTC 1 cut(s) 97
FaeI CATG 1 cut(s) 497
FaiI YATR 3 cut(s) 495, 648, 788
FaqI GGGAC 5 cut(s) 82, 138, 375, 494, 523
FatI CATG 1 cut(s) 493
FauI CCCGC 1 cut(s) 520
Fnu4HI GCNGC 6 cut(s) 213, 350, 353, 356, 727, 798
FokI GGATG 1 cut(s) 533
FriOI GRGCYC 1 cut(s) 806
Fsp4HI GCNGC 6 cut(s) 213, 350, 353, 356, 727, 798
FspBI CTAG 1 cut(s) 143
GlaI GCGC 1 cut(s) 656
GluI GCNGC 6 cut(s) 213, 350, 353, 356, 727, 798
HaeII RGCGCY 1 cut(s) 658
HaeIII GGCC 4 cut(s) 310, 315, 333, 427
HapII CCGG 6 cut(s) 101, 311, 359, 428, 438, 513
HgaI GACGC 1 cut(s) 299
HhaI GCGC 1 cut(s) 657
Hin1I GRCGYC 1 cut(s) 291
Hin1II CATG 1 cut(s) 497
Hin6I GCGC 1 cut(s) 655
HinP1I GCGC 1 cut(s) 655
HincII GTYRAC 3 cut(s) 109, 121, 247
HindII GTYRAC 3 cut(s) 109, 121, 247
HinfI GANTC 3 cut(s) 53, 231, 340
HpaII CCGG 6 cut(s) 101, 311, 359, 428, 438, 513
HphI GGTGA 1 cut(s) 48
Hpy166II GTNNAC 4 cut(s) 109, 121, 247, 687
Hpy188I TCNGA 6 cut(s) 46, 133, 397, 550, 642, 842
Hpy188III TCNNGA 1 cut(s) 678
Hpy8I GTNNAC 4 cut(s) 109, 121, 247, 687
Hpy99I CGWCG 2 cut(s) 293, 299
HpyAV CCTTC 3 cut(s) 406, 417, 547
HpyCH4III ACNGT 2 cut(s) 505, 691
HpyCH4IV ACGT 3 cut(s) 123, 336, 345
HpyCH4V TGCA 3 cut(s) 212, 407, 573
HpyF10VI GCNNNNNNNGC 5 cut(s) 36, 167, 355, 590, 723
HpyF3I CTNAG 3 cut(s) 130, 549, 696
HpySE526I ACGT 3 cut(s) 123, 336, 345
Hsp92I GRCGYC 1 cut(s) 291
Hsp92II CATG 1 cut(s) 497
HspAI GCGC 1 cut(s) 655
KroI GCCGGC 1 cut(s) 427
KroNI GCCGGC 1 cut(s) 429
Kzo9I GATC 2 cut(s) 434, 680
LmnI GCTCC 1 cut(s) 590
Lsp1109I GCAGC 2 cut(s) 224, 784
MabI ACCWGGT 1 cut(s) 619
MaeI CTAG 1 cut(s) 143
MaeII ACGT 3 cut(s) 123, 336, 345
MaeIII GTNAC 4 cut(s) 124, 499, 604, 691
MalI GATC 2 cut(s) 436, 682
MboI GATC 2 cut(s) 434, 680
MboII GAAGA 6 cut(s) 17, 271, 274, 277, 429, 618
MflI RGATCY 1 cut(s) 434
MhlI GDGCHC 1 cut(s) 806
MluCI AATT 3 cut(s) 325, 534, 765
MlyI GAGTC 2 cut(s) 47, 240
MroNI GCCGGC 1 cut(s) 427
MspA1I CMGCKG 1 cut(s) 797
MspI CCGG 6 cut(s) 101, 311, 359, 428, 438, 513
MspR9I CCNGG 6 cut(s) 101, 255, 312, 360, 378, 621
Mva1269I GAATGC 1 cut(s) 573
MvaI CCWGG 3 cut(s) 255, 378, 621
MwoI GCNNNNNNNGC 5 cut(s) 36, 167, 355, 590, 723
NaeI GCCGGC 1 cut(s) 429
NciI CCSGG 3 cut(s) 101, 312, 360
NdeII GATC 2 cut(s) 434, 680
NgoMIV GCCGGC 1 cut(s) 427
NlaIII CATG 1 cut(s) 497
NlaIV GGNNCC 4 cut(s) 154, 436, 511, 558
NmeAIII GCCGAG 1 cut(s) 15
NmuCI GTSAC 2 cut(s) 124, 691
NspI RCATGY 1 cut(s) 497
PaeR7I CTCGAG 1 cut(s) 561
PaqCI CACCTGC 1 cut(s) 464
PciI ACATGT 1 cut(s) 493
PcsI WCGNNNNNNNCGW 1 cut(s) 342
PctI GAATGC 1 cut(s) 573
PdiI GCCGGC 1 cut(s) 429
PfeI GAWTC 1 cut(s) 340
PkrI GCNGC 6 cut(s) 214, 351, 354, 357, 728, 799
PleI GAGTC 2 cut(s) 47, 239
PmaCI CACGTG 2 cut(s) 337, 346
PmlI CACGTG 2 cut(s) 337, 346
PpsI GAGTC 2 cut(s) 47, 239
Ppu21I YACGTR 2 cut(s) 337, 346
PpuMI RGGWCCY 1 cut(s) 557
PscI ACATGT 1 cut(s) 493
Psp5II RGGWCCY 1 cut(s) 557
Psp6I CCWGG 3 cut(s) 253, 376, 619
PspCI CACGTG 2 cut(s) 337, 346
PspGI CCWGG 3 cut(s) 253, 376, 619
PspN4I GGNNCC 4 cut(s) 154, 436, 511, 558
PspPI GGNCC 6 cut(s) 152, 313, 331, 510, 557, 749
PspPPI RGGWCCY 1 cut(s) 557
PspXI VCTCGAGB 1 cut(s) 561
PstNI CAGNNNCTG 1 cut(s) 695
PsuI RGATCY 1 cut(s) 434
PvuII CAGCTG 1 cut(s) 797
RsaI GTAC 2 cut(s) 204, 287
RsaNI GTAC 2 cut(s) 203, 286
SatI GCNGC 6 cut(s) 213, 350, 353, 356, 727, 798
Sau3AI GATC 2 cut(s) 434, 680
Sau96I GGNCC 6 cut(s) 152, 313, 331, 510, 557, 749
ScaI AGTACT 1 cut(s) 204
SchI GAGTC 2 cut(s) 47, 240
ScrFI CCNGG 6 cut(s) 101, 255, 312, 360, 378, 621
SduI GDGCHC 1 cut(s) 806
SexAI ACCWGGT 1 cut(s) 619
Sfr274I CTCGAG 1 cut(s) 561
SinI GGWCC 4 cut(s) 152, 510, 557, 749
SlaI CTCGAG 1 cut(s) 561
SmlI CTYRAG 3 cut(s) 304, 561, 730
SmoI CTYRAG 3 cut(s) 304, 561, 730
SpeI ACTAGT 1 cut(s) 142
Sse9I AATT 3 cut(s) 325, 534, 765
SsiI CCGC 8 cut(s) 87, 116, 350, 353, 356, 527, 633, 726
SspMI CTAG 1 cut(s) 143
StyD4I CCNGG 6 cut(s) 99, 253, 310, 358, 376, 619
StyI CCWWGG 1 cut(s) 235
TaaI ACNGT 2 cut(s) 505, 691
TaiI ACGT 3 cut(s) 126, 339, 348
TaqI TCGA 1 cut(s) 562
TasI AATT 3 cut(s) 325, 534, 765
TatI WGTACW 1 cut(s) 202
TauI GCSGC 4 cut(s) 352, 355, 358, 729
TfiI GAWTC 1 cut(s) 340
TscAI CASTG 1 cut(s) 700
TseFI GTSAC 2 cut(s) 124, 691
TseI GCWGC 2 cut(s) 212, 797
Tsp45I GTSAC 2 cut(s) 124, 691
TspGWI ACGGA 1 cut(s) 312
TspRI CASTG 1 cut(s) 700
VpaK11BI GGWCC 4 cut(s) 152, 510, 557, 749
XapI RAATTY 1 cut(s) 534
XceI RCATGY 1 cut(s) 497
XhoI CTCGAG 1 cut(s) 561
XspI CTAG 1 cut(s) 143
ZrmI AGTACT 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.