Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0015335

Physical Location & Seq

Genomic Coordinates Forward (+)

12655065 .. 12657464

Gene Structure 2400 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g19130.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1827 bp

ATGACAACCTCTACCTCCCCTTGGAACTCTCGCTTAAGAGACCTCGCAAAGCAATCCCTCTTCTCCGAAGCTCTCTCTCTTTACCGCCAAATGCTCCGATTCGGCCACCCACCCAACGCTTTCACTTTCCCCTTCGCTCTCAAATCCTGCGCCGCCCTCGCCCTCCCTCTCACCGGTTCCCTCCTCCATTCCCACGTTCTCAAAACCGGGTGCGACCCCGAACCCTTCGTACAGACTTCTCTCATTTCCATGTACTGCAAATGCTGTTTGACTGACGATGCACGCAAGGTGTTCGACGAAAGTCCCCAAAGGAAGTTGACTGTTTGCTACAATGCTTTGATATCTGGGCATGCTTCGAACTCGAAGTTGCGCGATGCGGTTTTGTTGTTTCGTAGAATGAGGGAGGAGGGTGTGGGGGTTAATTCGGTTACAATGTTGGGTTTGATTCCGGGGTGTGCGGCTCCAGGGCACTTGAGTCTTGGGATGTGCCTACATGGTTCTAGTGTGAAGTGTGGTTTGGATAGTGATTTGTCTGTGAGGAATTGTTTGCTTACAATGTATGTGAAATGTGGGTTGGTTGATAATGCAAGGAAGATCTTTGATGCGATGCCTGAAAAGGGTTTGATTACTTGGAATGCAATGATTTCCGGGTATGCGCAAAATGGGCTTGCTTCTCATGTTTTGAACCTGTACAGGGAGATGGAAGCTTGTGGTTTTTTCCCTGATCCTGTGACGCTTGTTGGTGTTCTATCGTCTTGTACTCACCTTGGGGCGCATGGGGTTGGACGTGAGGTAGAAAGACGGATTGAGTCTAGTGGATTTGGTTCCAATCCGTATTTGAAAAATGCTCTGATCAATATGTATACTAGGTGTGGTAATTTGGTGAGGGCTCATTCTATTTTTGATGTCATGCCTGAAAAAAGCTTAGTTACTTGGACAGCAATTATTGGTGGGTATGGGATGCATGGACATGGAGAGGTCGCATTGGAGCTCTTTGAGGAAATGATTGTGTCTGGAATCAGACCTGACAAAGCTGTGTTTGTGACTGTTCTGTCTGCCTGTAGCCATGCAGGATTGACTGATGAGGGACTTGAGTATTTTGCTGCGATGGAGAAAAACTATAGGTTGCAGCCTGGACCAGAGCATTATTCTTGCATGGTGGATCTTTTAGGGCGCGCAGGTCGGCTCAAGGAAGCAAAGGAGCTTATTGACTCAATGCAGGTTAAGCCTGATGGTGGAGTATGGGGAGCCCTCTTGGGTGCCTGTAAGATACATAAAAATGTAGAACTAGCAGAGATAGCTTTTGAACATGTCATCGAGCTTGAACCAACTAACAGTGGTTATTATGTGTTGATGTCGAATATATACTCTGATGCAAAGAATTTAGAAGGGATTTTAAAGGTTCGAGTTATGATGAAAGAGAGGCAGCTTAAAAAAGAGCCAGGTTGTAGCTATGTCGAGTGTAAAGGAAGAGTTCATGTTTTCTTGGCTGGGGATAACAGCCATTGTCAGACAGAAGATATATACAGCATATTGGATGAGTTAGAAAATTTAGCCCGGGAACTTGGCGTGTCCAATGAGAATGATGGAGAAAGAAACAAAGAGCGAGTTGGTATTCACAGTGAAAAATTGGCAATTGCATTTGGGCTCTTGAACACTGAACCCGGCACTGAGATCGTAGTGATAAAGAACCTGAGGGTGTGTGCAGACTGTCACTTGTTTATCAAGTCGATTAGCAAGATTGTGCAGCGTCAGTTTGTGGTCAGAGACGCGACTCGTTTTCACCATTTCAGAAATGGTATCTGTTCTTGTAAAGACTACTGGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016553 GO:0016554 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

609

Amino Acids

67.26

Weight (kDa)

7.05

Isoelectric Point (pI)

35.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	107 - 153	9.4e-09	PPR repeat family
PPR	PF01535	108 - 138	7.1e-07	PPR repeat
PPR_2	PF13041	208 - 253	1.5e-07	PPR repeat family
PPR	PF01535	209 - 239	5.1e-08	PPR repeat
PPR_2	PF13041	308 - 355	1.5e-09	PPR repeat family
PPR	PF01535	310 - 340	2e-07	PPR repeat
E_motif	PF20431	425 - 487	8.8e-20	E motif
DYW_deaminase	PF14432	529 - 608	7.8e-33	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Orthologous Genes (Group: OG0015335)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11460
fragaria_vesca	FvH4_6g19130
malus_domestica	MD14G1027300.v1.1
prunus_persica	Prupe.7G104400_v2.0.a1
pyrus_communis	pycom14g02600
rosa_chinensis	RchiOBHm_Chr3g0474241
rosa_laevigata	RLG00000023936 RLG00000023939
rosa_multiflora	Rmu_sc0004507.1_g000021 Rmu_sc0015051.1_g000006
rosa_roxburghii	Rroxscaffold_6G00407410
rosa_rugosa	Rorug03G0139800
rosa_samantha	Rh3AG190200 Rh3BG219500 Rh3DG215000
rosa_wichuraiana	Rw3G017350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1051
Acc16I	TGCGCA	1 cut(s)	657
Acc36I	ACCTGC	2 cut(s)	1169, 1210
AccB1I	GGYRCC	1 cut(s)	1259
AccI	GTMKAC	1 cut(s)	863
AccII	CGCG	3 cut(s)	372, 1176, 1772
AciI	CCGC	4 cut(s)	85, 153, 377, 458
AclWI	GGATC	2 cut(s)	719, 1170
AcoI	YGGCCR	1 cut(s)	103
AcsI	RAATTY	2 cut(s)	1381, 1549
AfaI	GTAC	4 cut(s)	231, 254, 692, 760
AfiI	CCNNNNNNNGG	5 cut(s)	21, 173, 617, 694, 1235
AflII	CTTAAG	1 cut(s)	34
AflIII	ACRYGT	1 cut(s)	1309
AgeI	ACCGGT	1 cut(s)	173
AgsI	TTSAA	5 cut(s)	685, 841, 1307, 1325, 1654
AjiI	CACGTC	1 cut(s)	788
AjnI	CCWGG	3 cut(s)	463, 1132, 1441
AjuI	GAANNNNNNNTTGG	4 cut(s)	500, 532, 557, 589
Alw21I	GWGCWC	1 cut(s)	993
Alw26I	GTCTC	2 cut(s)	33, 1761
AlwI	GGATC	2 cut(s)	719, 1170
Ama87I	CYCGRG	1 cut(s)	1557
AoxI	GGCC	1 cut(s)	103
ApeKI	GCWGC	4 cut(s)	1103, 1129, 1426, 1747
ApoI	RAATTY	2 cut(s)	1381, 1549
AsiGI	ACCGGT	1 cut(s)	173
AspLEI	GCGC	6 cut(s)	152, 372, 658, 775, 1176, 1178
AspS9I	GGNCC	1 cut(s)	1136
AsuC2I	CCSGG	6 cut(s)	208, 450, 649, 1558, 1559, 1665
AsuHPI	GGTGA	4 cut(s)	163, 755, 895, 1775
AsuII	TTCGAA	1 cut(s)	356
AvaI	CYCGRG	1 cut(s)	1557
AvaII	GGWCC	1 cut(s)	1136
AxyI	CCTNAGG	1 cut(s)	1694
BaeGI	GKGCMC	1 cut(s)	471
BanI	GGYRCC	1 cut(s)	1259
BanII	GRGCYC	4 cut(s)	892, 993, 1252, 1650
Bbv12I	GWGCWC	1 cut(s)	993
BbvI	GCAGC	4 cut(s)	1090, 1141, 1438, 1759
BccI	CCATC	4 cut(s)	694, 1102, 1226, 1580
BcgI	CGANNNNNNTGC	4 cut(s)	274, 308, 1657, 1691
BciT130I	CCWGG	3 cut(s)	465, 1134, 1443
BclI	TGATCA	1 cut(s)	852
BcnI	CCSGG	6 cut(s)	208, 450, 649, 1558, 1559, 1665
BcoDI	GTCTC	2 cut(s)	33, 1761
BfaI	CTAG	4 cut(s)	501, 813, 867, 1289
BfmI	CTRYAG	2 cut(s)	1060, 1120
BfrI	CTTAAG	1 cut(s)	34
BfuAI	ACCTGC	2 cut(s)	1169, 1210
BglII	AGATCT	1 cut(s)	594
BisI	GCNGC	6 cut(s)	153, 459, 1104, 1130, 1427, 1748
BlsI	GCNGC	6 cut(s)	154, 460, 1105, 1131, 1428, 1749
Bme1390I	CCNGG	9 cut(s)	208, 450, 465, 649, 1134, 1443, 1558, 1559, 1665
Bme18I	GGWCC	1 cut(s)	1136
BmeT110I	CYCGRG	1 cut(s)	1557
BmgBI	CACGTC	1 cut(s)	788
BmgT120I	GGNCC	1 cut(s)	1136
BmiI	GGNNCC	5 cut(s)	178, 462, 826, 1249, 1261
BmrFI	CCNGG	9 cut(s)	208, 450, 465, 649, 1134, 1443, 1558, 1559, 1665
BmsI	GCATC	6 cut(s)	268, 364, 592, 597, 951, 1363
BoxI	GACNNNNGTC	1 cut(s)	300
BpmI	CTGGAG	1 cut(s)	447
Bpu14I	TTCGAA	1 cut(s)	356
BpuEI	CTTGAG	3 cut(s)	493, 1112, 1172
BpuMI	CCSGG	6 cut(s)	208, 450, 649, 1558, 1559, 1665
BsaI	GGTCTC	1 cut(s)	33
BsaJI	CCNNGG	5 cut(s)	20, 449, 464, 766, 1557
BsaWI	WCCGGW	1 cut(s)	173
Bsc4I	CCNNNNNNNGG	5 cut(s)	21, 173, 617, 694, 1235
Bse118I	RCCGGY	1 cut(s)	173
Bse1I	ACTGG	1 cut(s)	1826
Bse21I	CCTNAGG	1 cut(s)	1694
Bse3DI	GCAATG	1 cut(s)	645
BseBI	CCWGG	3 cut(s)	465, 1134, 1443
BseDI	CCNNGG	5 cut(s)	20, 449, 464, 766, 1557
BseGI	GGATG	3 cut(s)	489, 966, 1543
BseLI	CCNNNNNNNGG	5 cut(s)	21, 173, 617, 694, 1235
BseMI	GCAATG	1 cut(s)	645
BseMII	CTCAG	2 cut(s)	1662, 1685
BseNI	ACTGG	1 cut(s)	1826
BsePI	GCGCGC	1 cut(s)	1174
BseRI	GAGGAG	2 cut(s)	173, 419
BseSI	GKGCMC	1 cut(s)	471
BseXI	GCAGC	4 cut(s)	1090, 1141, 1438, 1759
BseYI	CCCAGC	1 cut(s)	1490
BsgI	GTGCAG	2 cut(s)	1725, 1766
Bsh1236I	CGCG	3 cut(s)	372, 1176, 1772
BshFI	GGCC	1 cut(s)	105
BshNI	GGYRCC	1 cut(s)	1259
BshTI	ACCGGT	1 cut(s)	173
BsiHKAI	GWGCWC	1 cut(s)	993
BsiHKCI	CYCGRG	1 cut(s)	1557
BsiSI	CCGG	6 cut(s)	174, 207, 449, 648, 1558, 1665
BslFI	GGGAC	2 cut(s)	288, 1101
BslI	CCNNNNNNNGG	5 cut(s)	21, 173, 617, 694, 1235
BsmAI	GTCTC	2 cut(s)	33, 1761
BsmBI	CGTCTC	1 cut(s)	1761
BsmFI	GGGAC	2 cut(s)	288, 1101
BsmI	GAATGC	1 cut(s)	640
BsnI	GGCC	1 cut(s)	105
Bso31I	GGTCTC	1 cut(s)	33
BsoBI	CYCGRG	1 cut(s)	1557
Bsp119I	TTCGAA	1 cut(s)	356
Bsp1286I	GDGCHC	5 cut(s)	471, 892, 993, 1252, 1650
Bsp1407I	TGTACA	1 cut(s)	690
Bsp143I	GATC	5 cut(s)	594, 724, 852, 1162, 1674
BspACI	CCGC	4 cut(s)	85, 153, 377, 458
BspANI	GGCC	1 cut(s)	105
BspCNI	CTCAG	2 cut(s)	1663, 1686
BspFNI	CGCG	3 cut(s)	372, 1176, 1772
BspLI	GGNNCC	5 cut(s)	178, 462, 826, 1249, 1261
BspMI	ACCTGC	2 cut(s)	1169, 1210
BspPI	GGATC	2 cut(s)	719, 1170
BspT104I	TTCGAA	1 cut(s)	356
BspT107I	GGYRCC	1 cut(s)	1259
BspTI	CTTAAG	1 cut(s)	34
BspTNI	GGTCTC	1 cut(s)	33
BsrDI	GCAATG	1 cut(s)	645
BsrFI	RCCGGY	1 cut(s)	173
BsrGI	TGTACA	1 cut(s)	690
BsrI	ACTGG	1 cut(s)	1826
BssAI	RCCGGY	1 cut(s)	173
BssECI	CCNNGG	5 cut(s)	20, 449, 464, 766, 1557
BssHII	GCGCGC	1 cut(s)	1174
BssMI	GATC	5 cut(s)	594, 724, 852, 1162, 1674
BssNAI	GTATAC	1 cut(s)	864
BssT1I	CCWWGG	2 cut(s)	20, 766
Bst1107I	GTATAC	1 cut(s)	864
Bst2UI	CCWGG	3 cut(s)	465, 1134, 1443
Bst4CI	ACNGT	5 cut(s)	322, 1048, 1337, 1622, 1712
Bst6I	CTCTTC	2 cut(s)	65, 1465
BstAFI	CTTAAG	1 cut(s)	34
BstAUI	TGTACA	1 cut(s)	690
BstBI	TTCGAA	1 cut(s)	356
BstC8I	GCNNGC	4 cut(s)	283, 351, 669, 1176
BstDEI	CTNAG	3 cut(s)	925, 1671, 1694
BstF5I	GGATG	3 cut(s)	489, 966, 1543
BstFNI	CGCG	3 cut(s)	372, 1176, 1772
BstHHI	GCGC	6 cut(s)	152, 372, 658, 775, 1176, 1178
BstKTI	GATC	5 cut(s)	597, 727, 855, 1165, 1677
BstMAI	GTCTC	2 cut(s)	33, 1761
BstMBI	GATC	5 cut(s)	594, 724, 852, 1162, 1674
BstMWI	GCNNNNNNNGC	4 cut(s)	158, 664, 1225, 1298
BstNI	CCWGG	3 cut(s)	465, 1134, 1443
BstNSI	RCATGY	2 cut(s)	353, 1313
BstPAI	GACNNNNGTC	1 cut(s)	300
BstSCI	CCNGG	9 cut(s)	206, 448, 463, 647, 1132, 1441, 1556, 1557, 1663
BstSFI	CTRYAG	2 cut(s)	1060, 1120
BstSLI	GKGCMC	1 cut(s)	471
BstUI	CGCG	3 cut(s)	372, 1176, 1772
BstV1I	GCAGC	4 cut(s)	1090, 1141, 1438, 1759
BstX2I	RGATCY	2 cut(s)	594, 1162
BstYI	RGATCY	2 cut(s)	594, 1162
BstZ17I	GTATAC	1 cut(s)	864
Bsu36I	CCTNAGG	1 cut(s)	1694
BsuRI	GGCC	1 cut(s)	105
BtgZI	GCGATG	3 cut(s)	387, 620, 1121
BtrI	CACGTC	1 cut(s)	788
BtsCI	GGATG	3 cut(s)	489, 966, 1543
BtsIMutI	CAGTG	4 cut(s)	1342, 1627, 1656, 1668
BveI	ACCTGC	2 cut(s)	1169, 1210
Cac8I	GCNNGC	4 cut(s)	283, 351, 669, 1176
CfoI	GCGC	6 cut(s)	152, 372, 658, 775, 1176, 1178
Cfr10I	RCCGGY	1 cut(s)	173
Cfr13I	GGNCC	1 cut(s)	1136
Cfr9I	CCCGGG	1 cut(s)	1557
CseI	GACGC	3 cut(s)	742, 1739, 1778
Csp6I	GTAC	4 cut(s)	230, 253, 691, 759
CspAI	ACCGGT	1 cut(s)	173
CviQI	GTAC	4 cut(s)	230, 253, 691, 759
DdeI	CTNAG	3 cut(s)	925, 1671, 1694
DpnI	GATC	5 cut(s)	596, 726, 854, 1164, 1676
DpnII	GATC	5 cut(s)	594, 724, 852, 1162, 1674
DraI	TTTAAA	1 cut(s)	1398
DrdI	GACNNNNNNGTC	1 cut(s)	1051
DseDI	GACNNNNNNGTC	1 cut(s)	1051
EaeI	YGGCCR	1 cut(s)	103
Eam1104I	CTCTTC	2 cut(s)	65, 1465
EarI	CTCTTC	2 cut(s)	65, 1465
Ecl136II	GAGCTC	1 cut(s)	991
Eco130I	CCWWGG	2 cut(s)	20, 766
Eco24I	GRGCYC	4 cut(s)	892, 993, 1252, 1650
Eco31I	GGTCTC	1 cut(s)	33
Eco32I	GATATC	1 cut(s)	342
Eco47I	GGWCC	1 cut(s)	1136
Eco53kI	GAGCTC	1 cut(s)	991
Eco81I	CCTNAGG	1 cut(s)	1694
Eco88I	CYCGRG	1 cut(s)	1557
EcoICRI	GAGCTC	1 cut(s)	991
EcoRII	CCWGG	3 cut(s)	463, 1132, 1441
EcoRV	GATATC	1 cut(s)	342
EcoT14I	CCWWGG	2 cut(s)	20, 766
EcoT22I	ATGCAT	1 cut(s)	966
EcoT38I	GRGCYC	4 cut(s)	892, 993, 1252, 1650
ErhI	CCWWGG	2 cut(s)	20, 766
Esp3I	CGTCTC	1 cut(s)	1761
FaqI	GGGAC	2 cut(s)	288, 1101
FbaI	TGATCA	1 cut(s)	852
FblI	GTMKAC	1 cut(s)	863
Fnu4HI	GCNGC	6 cut(s)	153, 459, 1104, 1130, 1427, 1748
FokI	GGATG	3 cut(s)	496, 973, 1550
FriOI	GRGCYC	4 cut(s)	892, 993, 1252, 1650
Fsp4HI	GCNGC	6 cut(s)	153, 459, 1104, 1130, 1427, 1748
FspBI	CTAG	4 cut(s)	501, 813, 867, 1289
FspI	TGCGCA	1 cut(s)	657
GlaI	GCGC	6 cut(s)	151, 371, 657, 774, 1175, 1177
GluI	GCNGC	6 cut(s)	153, 459, 1104, 1130, 1427, 1748
GsaI	CCCAGC	1 cut(s)	1494
GsuI	CTGGAG	1 cut(s)	447
HaeIII	GGCC	1 cut(s)	105
HapII	CCGG	6 cut(s)	174, 207, 449, 648, 1558, 1665
HgaI	GACGC	3 cut(s)	742, 1739, 1778
HhaI	GCGC	6 cut(s)	152, 372, 658, 775, 1176, 1178
Hin6I	GCGC	6 cut(s)	150, 370, 656, 773, 1174, 1176
HinP1I	GCGC	6 cut(s)	150, 370, 656, 773, 1174, 1176
HincII	GTYRAC	1 cut(s)	318
HindII	GTYRAC	1 cut(s)	318
HindIII	AAGCTT	2 cut(s)	705, 922
HinfI	GANTC	7 cut(s)	99, 445, 475, 809, 1017, 1211, 1774
HpaII	CCGG	6 cut(s)	174, 207, 449, 648, 1558, 1665
HphI	GGTGA	4 cut(s)	163, 755, 895, 1775
Hpy166II	GTNNAC	2 cut(s)	318, 864
Hpy188I	TCNGA	8 cut(s)	67, 98, 852, 1022, 1372, 1512, 1766, 1793
Hpy188III	TCNNGA	2 cut(s)	1014, 1651
Hpy8I	GTNNAC	2 cut(s)	318, 864
Hpy99I	CGWCG	1 cut(s)	299
HpyAV	CCTTC	3 cut(s)	142, 235, 1382
HpyCH4III	ACNGT	5 cut(s)	322, 1048, 1337, 1622, 1712
HpyCH4IV	ACGT	2 cut(s)	195, 787
HpyF10VI	GCNNNNNNNGC	4 cut(s)	158, 664, 1225, 1298
HpyF3I	CTNAG	3 cut(s)	925, 1671, 1694
HpySE526I	ACGT	2 cut(s)	195, 787
HspAI	GCGC	6 cut(s)	150, 370, 656, 773, 1174, 1176
Ksp22I	TGATCA	1 cut(s)	852
Kzo9I	GATC	5 cut(s)	594, 724, 852, 1162, 1674
LmnI	GCTCC	5 cut(s)	99, 466, 988, 1201, 1247
Lsp1109I	GCAGC	4 cut(s)	1090, 1141, 1438, 1759
LweI	GCATC	6 cut(s)	268, 364, 592, 597, 951, 1363
MaeI	CTAG	4 cut(s)	501, 813, 867, 1289
MaeII	ACGT	2 cut(s)	195, 787
MaeIII	GTNAC	5 cut(s)	427, 730, 928, 1042, 1712
MalI	GATC	5 cut(s)	596, 726, 854, 1164, 1676
MboI	GATC	5 cut(s)	594, 724, 852, 1162, 1674
MboII	GAAGA	4 cut(s)	52, 604, 1482, 1529
MfeI	CAATTG	1 cut(s)	1635
MflI	RGATCY	2 cut(s)	594, 1162
MhlI	GDGCHC	5 cut(s)	471, 892, 993, 1252, 1650
MluCI	AATT	8 cut(s)	421, 541, 877, 942, 1381, 1549, 1628, 1635
MlyI	GAGTC	4 cut(s)	484, 818, 1205, 1768
MmeI	TCCRAC	1 cut(s)	763
Mph1103I	ATGCAT	1 cut(s)	966
MseI	TTAA	5 cut(s)	35, 420, 1224, 1397, 1431
MslI	CAYNNNNRTG	3 cut(s)	248, 969, 1278
MspCI	CTTAAG	1 cut(s)	34
MspI	CCGG	6 cut(s)	174, 207, 449, 648, 1558, 1665
MspR9I	CCNGG	9 cut(s)	208, 450, 465, 649, 1134, 1443, 1558, 1559, 1665
MunI	CAATTG	1 cut(s)	1635
Mva1269I	GAATGC	1 cut(s)	640
MvaI	CCWGG	3 cut(s)	465, 1134, 1443
MvnI	CGCG	3 cut(s)	372, 1176, 1772
MwoI	GCNNNNNNNGC	4 cut(s)	158, 664, 1225, 1298
NciI	CCSGG	6 cut(s)	208, 450, 649, 1558, 1559, 1665
NdeII	GATC	5 cut(s)	594, 724, 852, 1162, 1674
NlaIV	GGNNCC	5 cut(s)	178, 462, 826, 1249, 1261
NmuCI	GTSAC	3 cut(s)	730, 1042, 1712
NsbI	TGCGCA	1 cut(s)	657
NsiI	ATGCAT	1 cut(s)	966
NspI	RCATGY	2 cut(s)	353, 1313
NspV	TTCGAA	1 cut(s)	356
PaeI	GCATGC	1 cut(s)	353
PauI	GCGCGC	1 cut(s)	1174
PciI	ACATGT	1 cut(s)	1309
PctI	GAATGC	1 cut(s)	640
PfeI	GAWTC	3 cut(s)	99, 445, 1017
PinAI	ACCGGT	1 cut(s)	173
PkrI	GCNGC	6 cut(s)	154, 460, 1105, 1131, 1428, 1749
PleI	GAGTC	4 cut(s)	483, 817, 1205, 1768
PpsI	GAGTC	4 cut(s)	483, 817, 1205, 1768
PscI	ACATGT	1 cut(s)	1309
PshAI	GACNNNNGTC	1 cut(s)	300
Psp124BI	GAGCTC	1 cut(s)	993
Psp6I	CCWGG	3 cut(s)	463, 1132, 1441
PspFI	CCCAGC	1 cut(s)	1490
PspGI	CCWGG	3 cut(s)	463, 1132, 1441
PspN4I	GGNNCC	5 cut(s)	178, 462, 826, 1249, 1261
PspPI	GGNCC	1 cut(s)	1136
PsrI	GAACNNNNNNTAC	2 cut(s)	213, 245
PsuI	RGATCY	2 cut(s)	594, 1162
PteI	GCGCGC	1 cut(s)	1174
RsaI	GTAC	4 cut(s)	231, 254, 692, 760
RsaNI	GTAC	4 cut(s)	230, 253, 691, 759
RseI	CAYNNNNRTG	3 cut(s)	248, 969, 1278
SacI	GAGCTC	1 cut(s)	993
SaqAI	TTAA	5 cut(s)	35, 420, 1224, 1397, 1431
SatI	GCNGC	6 cut(s)	153, 459, 1104, 1130, 1427, 1748
Sau3AI	GATC	5 cut(s)	594, 724, 852, 1162, 1674
Sau96I	GGNCC	1 cut(s)	1136
SchI	GAGTC	4 cut(s)	484, 818, 1205, 1768
ScrFI	CCNGG	9 cut(s)	208, 450, 465, 649, 1134, 1443, 1558, 1559, 1665
SduI	GDGCHC	5 cut(s)	471, 892, 993, 1252, 1650
SfaNI	GCATC	6 cut(s)	268, 364, 592, 597, 951, 1363
SfcI	CTRYAG	2 cut(s)	1060, 1120
SfuI	TTCGAA	1 cut(s)	356
SinI	GGWCC	1 cut(s)	1136
SmaI	CCCGGG	1 cut(s)	1559
SmiMI	CAYNNNNRTG	3 cut(s)	248, 969, 1278
SmlI	CTYRAG	4 cut(s)	34, 472, 1091, 1187
SmoI	CTYRAG	4 cut(s)	34, 472, 1091, 1187
SphI	GCATGC	1 cut(s)	353
Sse9I	AATT	8 cut(s)	421, 541, 877, 942, 1381, 1549, 1628, 1635
SsiI	CCGC	4 cut(s)	85, 153, 377, 458
SspMI	CTAG	4 cut(s)	501, 813, 867, 1289
SstI	GAGCTC	1 cut(s)	993
StyD4I	CCNGG	9 cut(s)	206, 448, 463, 647, 1132, 1441, 1556, 1557, 1663
StyI	CCWWGG	2 cut(s)	20, 766
TaaI	ACNGT	5 cut(s)	322, 1048, 1337, 1622, 1712
TaiI	ACGT	2 cut(s)	198, 790
TaqI	TCGA	8 cut(s)	294, 356, 362, 1317, 1358, 1405, 1458, 1730
TasI	AATT	8 cut(s)	421, 541, 877, 942, 1381, 1549, 1628, 1635
TatI	WGTACW	3 cut(s)	252, 690, 758
TauI	GCSGC	2 cut(s)	155, 461
TfiI	GAWTC	3 cut(s)	99, 445, 1017
Tru1I	TTAA	5 cut(s)	35, 420, 1224, 1397, 1431
Tru9I	TTAA	5 cut(s)	35, 420, 1224, 1397, 1431
TscAI	CASTG	4 cut(s)	1342, 1627, 1663, 1675
TseFI	GTSAC	3 cut(s)	730, 1042, 1712
TseI	GCWGC	4 cut(s)	1103, 1129, 1426, 1747
Tsp45I	GTSAC	3 cut(s)	730, 1042, 1712
TspDTI	ATGAA	2 cut(s)	1430, 1466
TspGWI	ACGGA	2 cut(s)	817, 822
TspMI	CCCGGG	1 cut(s)	1557
TspRI	CASTG	4 cut(s)	1342, 1627, 1663, 1675
Vha464I	CTTAAG	1 cut(s)	34
VpaK11BI	GGWCC	1 cut(s)	1136
XapI	RAATTY	2 cut(s)	1381, 1549
XceI	RCATGY	2 cut(s)	353, 1313
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1793
XmaI	CCCGGG	1 cut(s)	1557
XmiI	GTMKAC	1 cut(s)	863
XspI	CTAG	4 cut(s)	501, 813, 867, 1289
Zsp2I	ATGCAT	1 cut(s)	966

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.