Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0015335

Physical Location & Seq

Genomic Coordinates Forward (+)

2475436 .. 2477256

Gene Structure 1821 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1027300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1821 bp

ATGTGGGAGTCTTCTTGGAACGCTCGATTACGAGAGCTATCGAAGCAATGTCTCTTCTTCGAAGCTCTTACTGTTTACCGCCAAATGCTCCGCCGTGGCCACTCGCCCAACGCCTTCACTTTCCCCTTCGCTCTCAAATCCTGCGCCGCGCTCTCGCTTCCTTTGACCGGCTCGCTCCTCCATTGCCACGTGCTCAAAACCGGCTGCGAACCCGAACCCTTTGTGCAAACTGCTCTCGTTTCGATGTACTGTAAGTGTTGCTTGGTTGACGATGCACGGAAGGTGTTCGATGAAAATCTCCTCTCGATGAAGCTGACTGTTTGCTACAATGCGTTGATAGCTGGGCACACTTCGAATTCGAGATTTTCTGACGCGGTTTTGTTGTTTCGTCGGATGAGGGAGGCGGGCGTGGCGGTTAATTCAGTTACGATGCTGGGTTTGGTTCCTGGGTGCGCTGCTCCGGTGTATTTGGGGCTTGGGATGTGCCTTCATGGCTCTAGTGTTAAGTGCGGCTTCGACATTGATTCGTCTGTGACGAATTGTTTGCTGACTATGTATGTGAAGTGTGGGTCGCTTGATCATGCAAGGAAGCTGTTTGATGCAATGCCTGAGAAGGGTTTGATTACTTGGAATGCGATGATTTCCGGGTACGCGCAAAACGGGCTCGCTACTCATGTTTTGAGCTTGTATAAGGAGATGGAGTCTTTTGGTGTTTGTCCTGATCCTGTGACACTAGTTGGTGTTCTTTCGTCTTGCACTCACCTTGGTGCACAGGGCATTGGACGTGAGGTAGAACGGCGGATAGAGTCTTGCGGATTTGGTTCTAATCCGTATTTGAATAATGCCCTGGTTAATATGTACGCTAGGTGTGGTAATTTAGTGAAGGCTCATACTATTTTCGATGCCATGCCTCAGAAAAGCTTGGTTTCCTGGACGGCGATTATAGGTGGGTATGGGTTGCATGGACAAGGAGAGGTTGCGTTGGAGCTCTTTGACAAAATGGTTATGACCAGAATTAGACCTGACAAAACTGTTTTCGTGACTGTTTTGTCTGCGTGTAGCCATGCGGGATTGACTGATAGGGGGTTGGAGTATTTTGCTGCGATGGAGAAAAGATATGGATTGCAGCCTGGTCCAGAGCATTACTCTTGCATGGTAGATCTTTTAGGTCGCGCAGGTCGACTCAAGGAAGCCAAGGAGCTAATTGAGTCCATGCCGGTAAATCCTGATGGTGCAGTATGGGGTGCCCTCTTAGGTGCCTGTAAAATTTATAAAAACGTAGAGCTGGCAGAGTTAGCATTTGAGCATGTCGTCGAGCTTGAGCCAACCAATATCGGTTACTATGTGTTGTTGTCGAATATATACTCCGATGCGAAGAATCTAGAGGGGGTTTTGAAGGTTCGAGTTATGATGAGGGAAAGGAAGCTCAAAAAGGAGCCAGGGTTTAGCTATGTCGAATGCAAAGGAACAGTTCATGTTTTCTTGGCTGGCGACAGAACCCATCGTCAGACTGAAGAAATATATAGGATGCTGGATGAATTAGAAAATTTAATGATGGAACCTGGCGTGTCTGATGAGAATGATCAAGGAAGAAACGAAGAGCAATTAGTTCACAGTGAAAAATTGGCAATTGCATTCGCGCTCTTAAATACTGCACCAGGGACCGAAATCGTAGTGATAAAGAACCTGAGGGTATGCGGAGACTGTCATTTGTTCATAAAGTTGGTAAGCAGGATTGTCGATCGCCAGTTTGTTGTCAGAGATGCAACTCGTTTTCACCATTTTAGGAATGGGGTCTGTTCTTGTAAAGACTACTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016553 GO:0016554 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

607

Amino Acids

67.37

Weight (kDa)

7.22

Isoelectric Point (pI)

36.7

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	106 - 152	1.1e-07	PPR repeat family
PPR_2	PF13041	207 - 252	5.1e-08	PPR repeat family
PPR	PF01535	208 - 238	1.1e-08	PPR repeat
PPR_2	PF13041	307 - 354	3.1e-07	PPR repeat family
E_motif	PF20431	424 - 486	3.7e-17	E motif
DYW_deaminase	PF14432	526 - 606	4.8e-33	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015335)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G11460
fragaria_vesca	FvH4_6g19130
malus_domestica	MD14G1027300.v1.1
prunus_persica	Prupe.7G104400_v2.0.a1
pyrus_communis	pycom14g02600
rosa_chinensis	RchiOBHm_Chr3g0474241
rosa_laevigata	RLG00000023936 RLG00000023939
rosa_multiflora	Rmu_sc0004507.1_g000021 Rmu_sc0015051.1_g000006
rosa_roxburghii	Rroxscaffold_6G00407410
rosa_rugosa	Rorug03G0139800
rosa_samantha	Rh3AG190200 Rh3BG219500 Rh3DG215000
rosa_wichuraiana	Rw3G017350

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1272
AasI	GACNNNNNNGTC	1 cut(s)	1048
Acc36I	ACCTGC	1 cut(s)	1166
AccB1I	GGYRCC	2 cut(s)	1244, 1256
AccI	GTMKAC	1 cut(s)	1180
AccII	CGCG	5 cut(s)	149, 374, 653, 1173, 1640
AclWI	GGATC	1 cut(s)	716
AcoI	YGGCCR	1 cut(s)	97
AcsI	RAATTY	3 cut(s)	355, 1266, 1546
AcuI	CTGAAG	1 cut(s)	1533
AcvI	CACGTG	1 cut(s)	190
AfaI	GTAC	3 cut(s)	248, 650, 860
AfiI	CCNNNNNNNGG	2 cut(s)	167, 614
AgsI	TTSAA	2 cut(s)	838, 1396
AhlI	ACTAGT	1 cut(s)	733
AjiI	CACGTC	1 cut(s)	785
AjnI	CCWGG	7 cut(s)	445, 846, 929, 1129, 1438, 1561, 1657
AleI	CACNNNNGTG	1 cut(s)	765
Alw21I	GWGCWC	3 cut(s)	195, 772, 990
Alw26I	GTCTC	2 cut(s)	56, 1695
Alw44I	GTGCAC	1 cut(s)	768
AlwI	GGATC	1 cut(s)	716
AoxI	GGCC	1 cut(s)	97
ApaLI	GTGCAC	1 cut(s)	768
ApeKI	GCWGC	4 cut(s)	204, 455, 1100, 1126
ApoI	RAATTY	3 cut(s)	355, 1266, 1546
ArsI	GACNNNNNNTTYG	2 cut(s)	526, 558
Asp700I	GAANNNNTTC	1 cut(s)	284
AspLEI	GCGC	6 cut(s)	146, 151, 455, 655, 1175, 1642
AspS9I	GGNCC	2 cut(s)	1133, 1662
AsuC2I	CCSGG	1 cut(s)	646
AsuHPI	GGTGA	2 cut(s)	752, 1769
AsuII	TTCGAA	2 cut(s)	60, 353
AvaII	GGWCC	2 cut(s)	1133, 1662
AxyI	CCTNAGG	1 cut(s)	1688
BaeGI	GKGCMC	3 cut(s)	348, 772, 1249
BalI	TGGCCA	1 cut(s)	99
BanI	GGYRCC	2 cut(s)	1244, 1256
BanII	GRGCYC	2 cut(s)	666, 990
BbrPI	CACGTG	1 cut(s)	190
BbsI	GAAGAC	1 cut(s)	3
Bbv12I	GWGCWC	3 cut(s)	195, 772, 990
BbvI	GCAGC	4 cut(s)	191, 442, 1087, 1138
BccI	CCATC	5 cut(s)	691, 1099, 1223, 1509, 1549
BceAI	ACGGC	3 cut(s)	78, 812, 951
BcgI	CGANNNNNNTGC	4 cut(s)	526, 560, 1720, 1754
BciT130I	CCWGG	7 cut(s)	447, 848, 931, 1131, 1440, 1563, 1659
BclI	TGATCA	2 cut(s)	577, 1582
BcnI	CCSGG	1 cut(s)	646
BcoDI	GTCTC	2 cut(s)	56, 1695
BcuI	ACTAGT	1 cut(s)	733
BfaI	CTAG	4 cut(s)	498, 734, 864, 1382
BfuAI	ACCTGC	1 cut(s)	1166
BglI	GCCNNNNNGGC	1 cut(s)	492
BglII	AGATCT	1 cut(s)	1159
BisI	GCNGC	6 cut(s)	147, 205, 456, 511, 1101, 1127
BlsI	GCNGC	6 cut(s)	148, 206, 457, 512, 1102, 1128
Bme1390I	CCNGG	8 cut(s)	447, 646, 848, 931, 1131, 1440, 1563, 1659
Bme18I	GGWCC	2 cut(s)	1133, 1662
BmgBI	CACGTC	1 cut(s)	785
BmgT120I	GGNCC	2 cut(s)	1133, 1662
BmiI	GGNNCC	6 cut(s)	444, 1246, 1258, 1437, 1560, 1663
BmrFI	CCNGG	8 cut(s)	447, 646, 848, 931, 1131, 1440, 1563, 1659
BmsI	GCATC	7 cut(s)	262, 420, 589, 892, 1360, 1518, 1753
BpiI	GAAGAC	1 cut(s)	3
BplI	GAGNNNNNCTC	2 cut(s)	1130, 1162
Bpu14I	TTCGAA	2 cut(s)	60, 353
BpuEI	CTTGAG	2 cut(s)	1169, 1340
BpuMI	CCSGG	1 cut(s)	646
BsaAI	YACGTR	1 cut(s)	190
BsaBI	GATNNNNATC	1 cut(s)	294
BsaJI	CCNNGG	7 cut(s)	94, 446, 763, 846, 1194, 1439, 1658
BsaWI	WCCGGW	1 cut(s)	460
BsaXI	ACNNNNNCTCC	4 cut(s)	686, 716, 1427, 1457
Bsc4I	CCNNNNNNNGG	2 cut(s)	167, 614
Bse118I	RCCGGY	3 cut(s)	167, 200, 1216
Bse1I	ACTGG	2 cut(s)	1747, 1820
Bse21I	CCTNAGG	1 cut(s)	1688
Bse3DI	GCAATG	3 cut(s)	53, 181, 609
Bse8I	GATNNNNATC	1 cut(s)	294
BseBI	CCWGG	7 cut(s)	447, 848, 931, 1131, 1440, 1563, 1659
BseDI	CCNNGG	7 cut(s)	94, 446, 763, 846, 1194, 1439, 1658
BseGI	GGATG	4 cut(s)	399, 486, 1533, 1540
BseJI	GATNNNNATC	1 cut(s)	294
BseLI	CCNNNNNNNGG	2 cut(s)	167, 614
BseMI	GCAATG	3 cut(s)	53, 181, 609
BseMII	CTCAG	3 cut(s)	600, 926, 1679
BseNI	ACTGG	2 cut(s)	1747, 1820
BseRI	GAGGAG	2 cut(s)	167, 290
BseSI	GKGCMC	3 cut(s)	348, 772, 1249
BseXI	GCAGC	4 cut(s)	191, 442, 1087, 1138
BseYI	CCCAGC	2 cut(s)	341, 433
BsgI	GTGCAG	2 cut(s)	1254, 1638
Bsh1236I	CGCG	5 cut(s)	149, 374, 653, 1173, 1640
Bsh1285I	CGRYCG	1 cut(s)	1744
BshFI	GGCC	1 cut(s)	99
BshNI	GGYRCC	2 cut(s)	1244, 1256
BsiEI	CGRYCG	1 cut(s)	1744
BsiHKAI	GWGCWC	3 cut(s)	195, 772, 990
BsiSI	CCGG	5 cut(s)	168, 201, 461, 645, 1217
BslFI	GGGAC	1 cut(s)	1675
BslI	CCNNNNNNNGG	2 cut(s)	167, 614
BsmAI	GTCTC	2 cut(s)	56, 1695
BsmFI	GGGAC	1 cut(s)	1675
BsmI	GAATGC	3 cut(s)	637, 1463, 1634
BsnI	GGCC	1 cut(s)	99
Bsp119I	TTCGAA	2 cut(s)	60, 353
Bsp1286I	GDGCHC	6 cut(s)	195, 348, 666, 772, 990, 1249
Bsp143I	GATC	5 cut(s)	577, 721, 1159, 1582, 1741
BspANI	GGCC	1 cut(s)	99
BspCNI	CTCAG	3 cut(s)	601, 925, 1680
BspFNI	CGCG	5 cut(s)	149, 374, 653, 1173, 1640
BspLI	GGNNCC	6 cut(s)	444, 1246, 1258, 1437, 1560, 1663
BspMI	ACCTGC	1 cut(s)	1166
BspPI	GGATC	1 cut(s)	716
BspQI	GCTCTTC	1 cut(s)	1593
BspT104I	TTCGAA	2 cut(s)	60, 353
BspT107I	GGYRCC	2 cut(s)	1244, 1256
BsrDI	GCAATG	3 cut(s)	53, 181, 609
BsrFI	RCCGGY	3 cut(s)	167, 200, 1216
BsrI	ACTGG	2 cut(s)	1747, 1820
BssAI	RCCGGY	3 cut(s)	167, 200, 1216
BssECI	CCNNGG	7 cut(s)	94, 446, 763, 846, 1194, 1439, 1658
BssMI	GATC	5 cut(s)	577, 721, 1159, 1582, 1741
BssT1I	CCWWGG	2 cut(s)	763, 1194
Bst2UI	CCWGG	7 cut(s)	447, 848, 931, 1131, 1440, 1563, 1659
Bst4CI	ACNGT	8 cut(s)	73, 251, 319, 1033, 1045, 1471, 1616, 1706
Bst6I	CTCTTC	2 cut(s)	59, 1593
BstBAI	YACGTR	1 cut(s)	190
BstBI	TTCGAA	2 cut(s)	60, 353
BstC8I	GCNNGC	5 cut(s)	173, 406, 666, 1287, 1489
BstDEI	CTNAG	4 cut(s)	609, 912, 1252, 1688
BstDSI	CCRYGG	1 cut(s)	94
BstF5I	GGATG	4 cut(s)	399, 486, 1533, 1540
BstFNI	CGCG	5 cut(s)	149, 374, 653, 1173, 1640
BstHHI	GCGC	6 cut(s)	146, 151, 455, 655, 1175, 1642
BstKTI	GATC	5 cut(s)	580, 724, 1162, 1585, 1744
BstMAI	GTCTC	2 cut(s)	56, 1695
BstMBI	GATC	5 cut(s)	577, 721, 1159, 1582, 1741
BstMCI	CGRYCG	1 cut(s)	1744
BstMWI	GCNNNNNNNGC	6 cut(s)	43, 338, 410, 492, 661, 1295
BstNI	CCWGG	7 cut(s)	447, 848, 931, 1131, 1440, 1563, 1659
BstNSI	RCATGY	1 cut(s)	1310
BstSCI	CCNGG	8 cut(s)	445, 644, 846, 929, 1129, 1438, 1561, 1657
BstSLI	GKGCMC	3 cut(s)	348, 772, 1249
BstUI	CGCG	5 cut(s)	149, 374, 653, 1173, 1640
BstV1I	GCAGC	4 cut(s)	191, 442, 1087, 1138
BstV2I	GAAGAC	1 cut(s)	3
BstX2I	RGATCY	1 cut(s)	1159
BstYI	RGATCY	1 cut(s)	1159
Bsu36I	CCTNAGG	1 cut(s)	1688
BsuRI	GGCC	1 cut(s)	99
BtgI	CCRYGG	1 cut(s)	94
BtgZI	GCGATG	2 cut(s)	650, 1118
BtrI	CACGTC	1 cut(s)	785
BtsCI	GGATG	4 cut(s)	399, 486, 1533, 1540
BtsIMutI	CAGTG	1 cut(s)	1621
BveI	ACCTGC	1 cut(s)	1166
Cac8I	GCNNGC	5 cut(s)	173, 406, 666, 1287, 1489
CfoI	GCGC	6 cut(s)	146, 151, 455, 655, 1175, 1642
Cfr10I	RCCGGY	3 cut(s)	167, 200, 1216
Cfr13I	GGNCC	2 cut(s)	1133, 1662
CseI	GACGC	1 cut(s)	380
Csp6I	GTAC	3 cut(s)	247, 649, 859
CviQI	GTAC	3 cut(s)	247, 649, 859
DdeI	CTNAG	4 cut(s)	609, 912, 1252, 1688
DpnI	GATC	5 cut(s)	579, 723, 1161, 1584, 1743
DpnII	GATC	5 cut(s)	577, 721, 1159, 1582, 1741
DrdI	GACNNNNNNGTC	1 cut(s)	1048
DseDI	GACNNNNNNGTC	1 cut(s)	1048
EaeI	YGGCCR	1 cut(s)	97
Eam1104I	CTCTTC	2 cut(s)	59, 1593
EarI	CTCTTC	2 cut(s)	59, 1593
EciI	GGCGGA	2 cut(s)	80, 814
Ecl136II	GAGCTC	1 cut(s)	988
Eco130I	CCWWGG	2 cut(s)	763, 1194
Eco24I	GRGCYC	2 cut(s)	666, 990
Eco47I	GGWCC	2 cut(s)	1133, 1662
Eco53kI	GAGCTC	1 cut(s)	988
Eco57I	CTGAAG	1 cut(s)	1533
Eco72I	CACGTG	1 cut(s)	190
Eco81I	CCTNAGG	1 cut(s)	1688
EcoICRI	GAGCTC	1 cut(s)	988
EcoRI	GAATTC	1 cut(s)	355
EcoRII	CCWGG	7 cut(s)	445, 846, 929, 1129, 1438, 1561, 1657
EcoT14I	CCWWGG	2 cut(s)	763, 1194
EcoT38I	GRGCYC	2 cut(s)	666, 990
ErhI	CCWWGG	2 cut(s)	763, 1194
FalI	AAGNNNNNCTT	4 cut(s)	245, 277, 497, 529
FaqI	GGGAC	1 cut(s)	1675
FauI	CCCGC	2 cut(s)	397, 1060
FbaI	TGATCA	2 cut(s)	577, 1582
FblI	GTMKAC	1 cut(s)	1180
Fnu4HI	GCNGC	6 cut(s)	147, 205, 456, 511, 1101, 1127
FokI	GGATG	4 cut(s)	406, 493, 1540, 1547
FriOI	GRGCYC	2 cut(s)	666, 990
Fsp4HI	GCNGC	6 cut(s)	147, 205, 456, 511, 1101, 1127
FspBI	CTAG	4 cut(s)	498, 734, 864, 1382
GlaI	GCGC	6 cut(s)	145, 150, 454, 654, 1174, 1641
GluI	GCNGC	6 cut(s)	147, 205, 456, 511, 1101, 1127
GsaI	CCCAGC	2 cut(s)	345, 437
HaeIII	GGCC	1 cut(s)	99
HapII	CCGG	5 cut(s)	168, 201, 461, 645, 1217
HgaI	GACGC	1 cut(s)	380
HhaI	GCGC	6 cut(s)	146, 151, 455, 655, 1175, 1642
Hin6I	GCGC	6 cut(s)	144, 149, 453, 653, 1173, 1640
HinP1I	GCGC	6 cut(s)	144, 149, 453, 653, 1173, 1640
HincII	GTYRAC	2 cut(s)	268, 1181
HindII	GTYRAC	2 cut(s)	268, 1181
HindIII	AAGCTT	1 cut(s)	919
HinfI	GANTC	7 cut(s)	8, 524, 701, 806, 1182, 1208, 1378
HpaII	CCGG	5 cut(s)	168, 201, 461, 645, 1217
HphI	GGTGA	2 cut(s)	752, 1769
Hpy166II	GTNNAC	5 cut(s)	76, 268, 770, 1181, 1612
Hpy188I	TCNGA	7 cut(s)	370, 393, 915, 1369, 1509, 1573, 1760
Hpy188III	TCNNGA	7 cut(s)	304, 360, 719, 1039, 1136, 1226, 1382
Hpy8I	GTNNAC	5 cut(s)	76, 268, 770, 1181, 1612
Hpy99I	CGWCG	2 cut(s)	393, 1316
HpyAV	CCTTC	7 cut(s)	124, 136, 274, 497, 607, 877, 1390
HpyCH4III	ACNGT	8 cut(s)	73, 251, 319, 1033, 1045, 1471, 1616, 1706
HpyCH4IV	ACGT	3 cut(s)	189, 784, 1278
HpyF10VI	GCNNNNNNNGC	6 cut(s)	43, 338, 410, 492, 661, 1295
HpyF3I	CTNAG	4 cut(s)	609, 912, 1252, 1688
HpySE526I	ACGT	3 cut(s)	189, 784, 1278
HspAI	GCGC	6 cut(s)	144, 149, 453, 653, 1173, 1640
Ksp22I	TGATCA	2 cut(s)	577, 1582
Kzo9I	GATC	5 cut(s)	577, 721, 1159, 1582, 1741
LguI	GCTCTTC	1 cut(s)	1593
LmnI	GCTCC	6 cut(s)	93, 180, 463, 985, 1198, 1435
Lsp1109I	GCAGC	4 cut(s)	191, 442, 1087, 1138
LweI	GCATC	7 cut(s)	262, 420, 589, 892, 1360, 1518, 1753
MaeI	CTAG	4 cut(s)	498, 734, 864, 1382
MaeII	ACGT	3 cut(s)	189, 784, 1278
MaeIII	GTNAC	5 cut(s)	424, 532, 727, 1039, 1337
MalI	GATC	5 cut(s)	579, 723, 1161, 1584, 1743
MboI	GATC	5 cut(s)	577, 721, 1159, 1582, 1741
MboII	GAAGA	7 cut(s)	3, 46, 49, 1387, 1526, 1602, 1610
MfeI	CAATTG	1 cut(s)	1629
MflI	RGATCY	1 cut(s)	1159
MhlI	GDGCHC	6 cut(s)	195, 348, 666, 772, 990, 1249
MlsI	TGGCCA	1 cut(s)	99
MluNI	TGGCCA	1 cut(s)	99
MlyI	GAGTC	5 cut(s)	17, 710, 815, 1176, 1217
MmeI	TCCRAC	3 cut(s)	371, 963, 1068
Mox20I	TGGCCA	1 cut(s)	99
MroXI	GAANNNNTTC	1 cut(s)	284
MscI	TGGCCA	1 cut(s)	99
MseI	TTAA	5 cut(s)	417, 504, 852, 1550, 1646
MslI	CAYNNNNRTG	1 cut(s)	765
Msp20I	TGGCCA	1 cut(s)	99
MspI	CCGG	5 cut(s)	168, 201, 461, 645, 1217
MspR9I	CCNGG	8 cut(s)	447, 646, 848, 931, 1131, 1440, 1563, 1659
MteI	GCGCNGCGC	1 cut(s)	147
MunI	CAATTG	1 cut(s)	1629
Mva1269I	GAATGC	3 cut(s)	637, 1463, 1634
MvaI	CCWGG	7 cut(s)	447, 848, 931, 1131, 1440, 1563, 1659
MvnI	CGCG	5 cut(s)	149, 374, 653, 1173, 1640
MwoI	GCNNNNNNNGC	6 cut(s)	43, 338, 410, 492, 661, 1295
NciI	CCSGG	1 cut(s)	646
NdeII	GATC	5 cut(s)	577, 721, 1159, 1582, 1741
NlaIV	GGNNCC	6 cut(s)	444, 1246, 1258, 1437, 1560, 1663
NmuCI	GTSAC	3 cut(s)	532, 727, 1039
NspI	RCATGY	1 cut(s)	1310
NspV	TTCGAA	2 cut(s)	60, 353
OliI	CACNNNNGTG	1 cut(s)	765
PciSI	GCTCTTC	1 cut(s)	1593
PcsI	WCGNNNNNNNCGW	2 cut(s)	533, 1177
PctI	GAATGC	3 cut(s)	637, 1463, 1634
PdmI	GAANNNNTTC	1 cut(s)	284
PfeI	GAWTC	2 cut(s)	524, 1378
PfoI	TCCNGGA	1 cut(s)	929
PkrI	GCNGC	6 cut(s)	148, 206, 457, 512, 1102, 1128
Ple19I	CGATCG	1 cut(s)	1744
PleI	GAGTC	5 cut(s)	16, 709, 814, 1176, 1216
PmaCI	CACGTG	1 cut(s)	190
PmlI	CACGTG	1 cut(s)	190
PpsI	GAGTC	5 cut(s)	16, 709, 814, 1176, 1216
Ppu21I	YACGTR	1 cut(s)	190
PsiI	TTATAA	1 cut(s)	1272
Psp124BI	GAGCTC	1 cut(s)	990
Psp6I	CCWGG	7 cut(s)	445, 846, 929, 1129, 1438, 1561, 1657
PspCI	CACGTG	1 cut(s)	190
PspFI	CCCAGC	2 cut(s)	341, 433
PspGI	CCWGG	7 cut(s)	445, 846, 929, 1129, 1438, 1561, 1657
PspN4I	GGNNCC	6 cut(s)	444, 1246, 1258, 1437, 1560, 1663
PspPI	GGNCC	2 cut(s)	1133, 1662
PsuI	RGATCY	1 cut(s)	1159
PvuI	CGATCG	1 cut(s)	1744
RsaI	GTAC	3 cut(s)	248, 650, 860
RsaNI	GTAC	3 cut(s)	247, 649, 859
RseI	CAYNNNNRTG	1 cut(s)	765
SacI	GAGCTC	1 cut(s)	990
SalI	GTCGAC	1 cut(s)	1179
SapI	GCTCTTC	1 cut(s)	1593
SaqAI	TTAA	5 cut(s)	417, 504, 852, 1550, 1646
SatI	GCNGC	6 cut(s)	147, 205, 456, 511, 1101, 1127
Sau3AI	GATC	5 cut(s)	577, 721, 1159, 1582, 1741
Sau96I	GGNCC	2 cut(s)	1133, 1662
SchI	GAGTC	5 cut(s)	17, 710, 815, 1176, 1217
ScrFI	CCNGG	8 cut(s)	447, 646, 848, 931, 1131, 1440, 1563, 1659
SduI	GDGCHC	6 cut(s)	195, 348, 666, 772, 990, 1249
SfaNI	GCATC	7 cut(s)	262, 420, 589, 892, 1360, 1518, 1753
SfuI	TTCGAA	2 cut(s)	60, 353
SinI	GGWCC	2 cut(s)	1133, 1662
SmiMI	CAYNNNNRTG	1 cut(s)	765
SmlI	CTYRAG	2 cut(s)	1184, 1319
SmoI	CTYRAG	2 cut(s)	1184, 1319
SpeI	ACTAGT	1 cut(s)	733
SspMI	CTAG	4 cut(s)	498, 734, 864, 1382
SstI	GAGCTC	1 cut(s)	990
StyD4I	CCNGG	8 cut(s)	445, 644, 846, 929, 1129, 1438, 1561, 1657
StyI	CCWWGG	2 cut(s)	763, 1194
TaaI	ACNGT	8 cut(s)	73, 251, 319, 1033, 1045, 1471, 1616, 1706
TaiI	ACGT	3 cut(s)	192, 787, 1281
TaqII	GACCGA	1 cut(s)	1679
TatI	WGTACW	1 cut(s)	246
TauI	GCSGC	2 cut(s)	149, 513
TfiI	GAWTC	2 cut(s)	524, 1378
Tru1I	TTAA	5 cut(s)	417, 504, 852, 1550, 1646
Tru9I	TTAA	5 cut(s)	417, 504, 852, 1550, 1646
TscAI	CASTG	1 cut(s)	1621
TseFI	GTSAC	3 cut(s)	532, 727, 1039
TseI	GCWGC	4 cut(s)	204, 455, 1100, 1126
Tsp45I	GTSAC	3 cut(s)	532, 727, 1039
TspDTI	ATGAA	6 cut(s)	306, 323, 479, 1463, 1551, 1705
TspGWI	ACGGA	2 cut(s)	292, 819
TspRI	CASTG	1 cut(s)	1621
VneI	GTGCAC	1 cut(s)	768
VpaK11BI	GGWCC	2 cut(s)	1133, 1662
XapI	RAATTY	3 cut(s)	355, 1266, 1546
XbaI	TCTAGA	1 cut(s)	1381
XceI	RCATGY	1 cut(s)	1310
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1787
XmiI	GTMKAC	1 cut(s)	1180
XmnI	GAANNNNTTC	1 cut(s)	284
XspI	CTAG	4 cut(s)	498, 734, 864, 1382

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.