Rorug03G0139800

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0011795
Physical Location & Seq
Reverse (-)
11499225 .. 11501915
2691 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0139800.1

Sequence Viewer

Length: 975 bp
ATGGACAAATTGGTTAGCCTGCCATGCTCATCAGACCTGAAGATGGCTTTGTTCTCGTCCGGGCCTCGCAGGCCGCCGTCATACTCTTCCTCCACAGTTTCCCTCCCCATCCGCACCCTCGGATCCGCAAGGTCCTATGCTAAGCGCCGTGAAGTCAACCGCACAAAACCTTCTCCAGCATCCCCGAAACTGAGGGCCACACAGAATCTCATTAAAGGGCTCAAGTCTGAGATCACAACTGTTGAGGAGGATTTAGAAAGATTTCGTGCAGTAGAGGGTTCAGACGAGTTTCCTTTTGAAATAGTCACTCGTTTAGGATATGGCCTAAAAACCACGGAGACTGAACTCAAAAGAGAACTTGATGATGAAATCATCCGAGTGTCAGTTGAGTATGATACTGATGAAGAAGATGATGATGAGGAAGATGATGAAGAAGAAGATGATGATGATGAAGATGAAGATGAAGATGAAGATGAAGATGAAGATGAAGATGAAGATGAAGATGAAGATGAAGATGATGATGATGATGATGATGATGATGATGAAGATGATGAAGATGATGATGAAGAAGATGAAGATGAAGATGAAGAAGATGATGATGATGAAGATGATGAAGAAGAAGATGAAGAAGAAGATTCTGGCATTCCAATGAAAGTGAGCATCTTCCAAAGAAGTACTGGACAGCAGCTGGAGTTGGATGTCACTGCTTTCCATGAGGAGATTTGCGTCGACCACTTTTCAATCAAGAAACTGGAGCATTCAGACCGTGATTTCACCAAAACTGGCGGCGAGTACTTGGCTAACTTACAGAAGGAATTAGTTAAATACCTTGAGGTTAAAGGGGTCACGCCCAACACAATTGAATTATGGAAGGCCAACTTGAAGAACAAAAAAGGCAGAGAGTCCCTGTTGAACATGAAAAACTTCAAGGCTTTTATGGAACGAAGCACCGTTTTCACCGGTGGTAAGAAATAG

Protein Analysis

324

Amino Acids

37.3

Weight (kDa)

4.05

Isoelectric Point (pI)

58.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAM33 PF02330 176 - 312 2.7e-11 Mitochondrial glycoprotein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011795)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11490
fragaria_vesca FvH4_6g19150
malus_domestica MD12G1027300.v1.1 MD14G1027500.v1.1
prunus_persica Prupe.7G104200_v2.0.a1
pyrus_communis pycom12g02620 pycom14g02620
rosa_chinensis RchiOBHm_Chr3g0474261
rosa_laevigata RLG00000023935
rosa_multiflora Rmu_sc0001803.1_g000025 Rmu_sc0004507.1_g000020 Rmu_sc0029669.1_g000001
rosa_roxburghii Rroxscaffold_6G00407400
rosa_rugosa Rorug03G0139800
rosa_samantha Rh3AG190400 Rh3BG219600 Rh3CG214800 Rh3DG215100
rosa_wichuraiana Rw3G017370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 729
AciI CCGC 5 cut(s) 74, 112, 126, 160, 786
AclWI GGATC 2 cut(s) 117, 130
AcuI CTGAAG 1 cut(s) 59
AfaI GTAC 2 cut(s) 676, 794
AfiI CCNNNNNNNGG 1 cut(s) 43
AgeI ACCGGT 1 cut(s) 959
AgsI TTSAA 6 cut(s) 299, 741, 863, 883, 913, 928
AluBI AGCT 1 cut(s) 688
AluI AGCT 1 cut(s) 688
Alw26I GTCTC 1 cut(s) 332
AlwI GGATC 2 cut(s) 117, 130
AlwNI CAGNNNCTG 1 cut(s) 688
AoxI GGCC 5 cut(s) 62, 71, 195, 322, 873
ApeKI GCWGC 1 cut(s) 685
AsiGI ACCGGT 1 cut(s) 959
Asp700I GAANNNNTTC 2 cut(s) 261, 923
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 3 cut(s) 62, 132, 195
AsuC2I CCSGG 1 cut(s) 61
AsuHPI GGTGA 2 cut(s) 766, 949
AvaII GGWCC 1 cut(s) 132
BamHI GGATCC 1 cut(s) 122
BanII GRGCYC 1 cut(s) 222
BbvI GCAGC 1 cut(s) 697
BccI CCATC 2 cut(s) 37, 116
BceAI ACGGC 2 cut(s) 61, 132
BcnI CCSGG 1 cut(s) 61
BcoDI GTCTC 1 cut(s) 332
BfoI RGCGCY 1 cut(s) 148
BglI GCCNNNNNGGC 1 cut(s) 70
BisI GCNGC 3 cut(s) 74, 686, 787
BlpI GCTNAGC 1 cut(s) 141
BlsI GCNGC 3 cut(s) 75, 687, 788
BmcAI AGTACT 2 cut(s) 676, 794
Bme1390I CCNGG 1 cut(s) 61
Bme18I GGWCC 1 cut(s) 132
BmgT120I GGNCC 3 cut(s) 62, 132, 195
BmiI GGNNCC 1 cut(s) 124
BmrFI CCNGG 1 cut(s) 61
BmsI GCATC 2 cut(s) 188, 669
BpmI CTGGAG 3 cut(s) 159, 710, 773
Bpu1102I GCTNAGC 1 cut(s) 141
BpuEI CTTGAG 2 cut(s) 206, 851
BpuMI CCSGG 1 cut(s) 61
BsaJI CCNNGG 2 cut(s) 118, 333
BsaWI WCCGGW 1 cut(s) 959
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bsc4I CCNNNNNNNGG 1 cut(s) 43
Bse118I RCCGGY 1 cut(s) 959
Bse1I ACTGG 3 cut(s) 682, 756, 787
BseDI CCNNGG 2 cut(s) 118, 333
BseGI GGATG 4 cut(s) 108, 179, 372, 703
BseLI CCNNNNNNNGG 1 cut(s) 43
BseMII CTCAG 2 cut(s) 182, 219
BseNI ACTGG 3 cut(s) 682, 756, 787
BseRI GAGGAG 2 cut(s) 260, 731
BseXI GCAGC 1 cut(s) 697
BsgI GTGCAG 1 cut(s) 288
BshFI GGCC 5 cut(s) 64, 73, 197, 324, 875
BshTI ACCGGT 1 cut(s) 959
BsiSI CCGG 2 cut(s) 60, 960
BslFI GGGAC 1 cut(s) 889
BslI CCNNNNNNNGG 1 cut(s) 43
BsmAI GTCTC 1 cut(s) 332
BsmFI GGGAC 1 cut(s) 889
BsmI GAATGC 2 cut(s) 642, 757
BsnI GGCC 5 cut(s) 64, 73, 197, 324, 875
Bsp1286I GDGCHC 1 cut(s) 222
Bsp143I GATC 2 cut(s) 122, 231
Bsp1720I GCTNAGC 1 cut(s) 141
BspACI CCGC 5 cut(s) 74, 112, 126, 160, 786
BspANI GGCC 5 cut(s) 64, 73, 197, 324, 875
BspCNI CTCAG 2 cut(s) 183, 220
BspLI GGNNCC 1 cut(s) 124
BspPI GGATC 2 cut(s) 117, 130
BsrFI RCCGGY 1 cut(s) 959
BsrI ACTGG 3 cut(s) 682, 756, 787
BssAI RCCGGY 1 cut(s) 959
BssECI CCNNGG 2 cut(s) 118, 333
BssMI GATC 2 cut(s) 122, 231
Bst4CI ACNGT 4 cut(s) 97, 241, 767, 952
Bst6I CTCTTC 1 cut(s) 91
BstC8I GCNNGC 2 cut(s) 20, 71
BstDEI CTNAG 3 cut(s) 141, 191, 228
BstDSI CCRYGG 1 cut(s) 333
BstF5I GGATG 4 cut(s) 108, 179, 372, 703
BstH2I RGCGCY 1 cut(s) 148
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 2 cut(s) 125, 234
BstMAI GTCTC 1 cut(s) 332
BstMBI GATC 2 cut(s) 122, 231
BstMWI GCNNNNNNNGC 2 cut(s) 24, 70
BstSCI CCNGG 1 cut(s) 59
BstV1I GCAGC 1 cut(s) 697
BstX2I RGATCY 1 cut(s) 122
BstYI RGATCY 1 cut(s) 122
BsuRI GGCC 5 cut(s) 64, 73, 197, 324, 875
BtgI CCRYGG 1 cut(s) 333
BtsCI GGATG 4 cut(s) 108, 179, 372, 703
BtsI GCAGTG 1 cut(s) 702
BtsIMutI CAGTG 1 cut(s) 702
Cac8I GCNNGC 2 cut(s) 20, 71
CaiI CAGNNNCTG 1 cut(s) 688
CfoI GCGC 1 cut(s) 147
Cfr10I RCCGGY 1 cut(s) 959
Cfr13I GGNCC 3 cut(s) 62, 132, 195
CseI GACGC 1 cut(s) 715
Csp6I GTAC 2 cut(s) 675, 793
CspAI ACCGGT 1 cut(s) 959
CviAII CATG 3 cut(s) 24, 713, 916
CviQI GTAC 2 cut(s) 675, 793
DdeI CTNAG 3 cut(s) 141, 191, 228
DpnI GATC 2 cut(s) 124, 233
DpnII GATC 2 cut(s) 122, 231
Eam1104I CTCTTC 1 cut(s) 91
EarI CTCTTC 1 cut(s) 91
Eco24I GRGCYC 1 cut(s) 222
Eco47I GGWCC 1 cut(s) 132
Eco57I CTGAAG 1 cut(s) 59
EcoO109I RGGNCCY 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 222
FaeI CATG 3 cut(s) 27, 716, 919
FaiI YATR 9 cut(s) 25, 82, 138, 321, 393, 714, 868, 917, 938
FalI AAGNNNNNCTT 2 cut(s) 863, 895
FaqI GGGAC 1 cut(s) 889
FatI CATG 3 cut(s) 23, 712, 915
FblI GTMKAC 1 cut(s) 729
Fnu4HI GCNGC 3 cut(s) 74, 686, 787
FokI GGATG 4 cut(s) 95, 166, 359, 710
FriOI GRGCYC 1 cut(s) 222
Fsp4HI GCNGC 3 cut(s) 74, 686, 787
GlaI GCGC 1 cut(s) 146
GluI GCNGC 3 cut(s) 74, 686, 787
GsuI CTGGAG 3 cut(s) 159, 710, 773
HaeII RGCGCY 1 cut(s) 148
HaeIII GGCC 5 cut(s) 64, 73, 197, 324, 875
HapII CCGG 2 cut(s) 60, 960
HgaI GACGC 1 cut(s) 715
HhaI GCGC 1 cut(s) 147
Hin1II CATG 3 cut(s) 27, 716, 919
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HincII GTYRAC 2 cut(s) 157, 730
HindII GTYRAC 2 cut(s) 157, 730
HinfI GANTC 3 cut(s) 205, 635, 902
HpaII CCGG 2 cut(s) 60, 960
HphI GGTGA 2 cut(s) 766, 949
Hpy166II GTNNAC 2 cut(s) 157, 730
Hpy188I TCNGA 6 cut(s) 34, 122, 229, 283, 377, 763
Hpy188III TCNNGA 1 cut(s) 745
Hpy8I GTNNAC 2 cut(s) 157, 730
Hpy99I CGWCG 1 cut(s) 731
HpyAV CCTTC 3 cut(s) 180, 805, 865
HpyCH4III ACNGT 4 cut(s) 97, 241, 767, 952
HpyCH4V TGCA 1 cut(s) 269
HpyF10VI GCNNNNNNNGC 2 cut(s) 24, 70
HpyF3I CTNAG 3 cut(s) 141, 191, 228
Hsp92II CATG 3 cut(s) 27, 716, 919
HspAI GCGC 1 cut(s) 145
Kzo9I GATC 2 cut(s) 122, 231
LmnI GCTCC 1 cut(s) 754
Lsp1109I GCAGC 1 cut(s) 697
LweI GCATC 2 cut(s) 188, 669
MaeIII GTNAC 3 cut(s) 304, 700, 844
MalI GATC 2 cut(s) 124, 233
MboI GATC 2 cut(s) 122, 231
MfeI CAATTG 1 cut(s) 858
MflI RGATCY 1 cut(s) 122
MhlI GDGCHC 1 cut(s) 222
MluCI AATT 4 cut(s) 8, 815, 858, 863
MlyI GAGTC 1 cut(s) 911
MmeI TCCRAC 1 cut(s) 675
MroXI GAANNNNTTC 2 cut(s) 261, 923
MseI TTAA 3 cut(s) 213, 822, 837
MslI CAYNNNNRTG 2 cut(s) 377, 647
MspA1I CMGCKG 1 cut(s) 688
MspI CCGG 2 cut(s) 60, 960
MspR9I CCNGG 1 cut(s) 61
MunI CAATTG 1 cut(s) 858
Mva1269I GAATGC 2 cut(s) 642, 757
MwoI GCNNNNNNNGC 2 cut(s) 24, 70
NciI CCSGG 1 cut(s) 61
NdeII GATC 2 cut(s) 122, 231
NlaIII CATG 3 cut(s) 27, 716, 919
NlaIV GGNNCC 1 cut(s) 124
NmuCI GTSAC 3 cut(s) 304, 700, 844
PctI GAATGC 2 cut(s) 642, 757
PdmI GAANNNNTTC 2 cut(s) 261, 923
PfeI GAWTC 2 cut(s) 205, 635
PinAI ACCGGT 1 cut(s) 959
PkrI GCNGC 3 cut(s) 75, 687, 788
PleI GAGTC 1 cut(s) 910
PpsI GAGTC 1 cut(s) 910
PpuMI RGGWCCY 1 cut(s) 132
Psp5II RGGWCCY 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 124
PspPI GGNCC 3 cut(s) 62, 132, 195
PspPPI RGGWCCY 1 cut(s) 132
PstNI CAGNNNCTG 1 cut(s) 688
PsuI RGATCY 1 cut(s) 122
PvuII CAGCTG 1 cut(s) 688
RsaI GTAC 2 cut(s) 676, 794
RsaNI GTAC 2 cut(s) 675, 793
RseI CAYNNNNRTG 2 cut(s) 377, 647
SalI GTCGAC 1 cut(s) 728
SaqAI TTAA 3 cut(s) 213, 822, 837
SatI GCNGC 3 cut(s) 74, 686, 787
Sau3AI GATC 2 cut(s) 122, 231
Sau96I GGNCC 3 cut(s) 62, 132, 195
ScaI AGTACT 2 cut(s) 676, 794
SchI GAGTC 1 cut(s) 911
ScrFI CCNGG 1 cut(s) 61
SduI GDGCHC 1 cut(s) 222
SetI ASST 6 cut(s) 39, 134, 172, 690, 831, 837
SfaNI GCATC 2 cut(s) 188, 669
SfiI GGCCNNNNNGGCC 1 cut(s) 70
SgrAI CRCCGGYG 1 cut(s) 959
SinI GGWCC 1 cut(s) 132
SmiMI CAYNNNNRTG 2 cut(s) 377, 647
SmlI CTYRAG 2 cut(s) 221, 830
SmoI CTYRAG 2 cut(s) 221, 830
Sse9I AATT 4 cut(s) 8, 815, 858, 863
SsiI CCGC 5 cut(s) 74, 112, 126, 160, 786
StyD4I CCNGG 1 cut(s) 59
TaaI ACNGT 4 cut(s) 97, 241, 767, 952
TaqI TCGA 1 cut(s) 729
TasI AATT 4 cut(s) 8, 815, 858, 863
TatI WGTACW 2 cut(s) 674, 792
TauI GCSGC 2 cut(s) 76, 789
TfiI GAWTC 2 cut(s) 205, 635
Tru1I TTAA 3 cut(s) 213, 822, 837
Tru9I TTAA 3 cut(s) 213, 822, 837
TscAI CASTG 1 cut(s) 709
TseFI GTSAC 3 cut(s) 304, 700, 844
TseI GCWGC 1 cut(s) 685
Tsp45I GTSAC 3 cut(s) 304, 700, 844
TspGWI ACGGA 1 cut(s) 350
TspRI CASTG 1 cut(s) 709
VpaK11BI GGWCC 1 cut(s) 132
XcmI CCANNNNNNNNNTGG 1 cut(s) 674
XmiI GTMKAC 1 cut(s) 729
XmnI GAANNNNTTC 2 cut(s) 261, 923
ZrmI AGTACT 2 cut(s) 676, 794
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.