RLG00000023935

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0011795
Physical Location & Seq
Forward (+)
30240354 .. 30247979
7626 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023935

Sequence Viewer

Length: 2568 bp
ATGACTGAGGTACTCCAATCCCCATCTCACTTCCCTTCACCTTCAAGCTCTTCCCCACCCACAAGTAATGATGGCCCATACCCACATGCCCTTATTACCAGTCCTTCCGTAGGTGAGAGCCTGCAAGCCCATTTGGTTTCAGAGGAGGAAGAACAAGAAGAAGAAGAAGAAGAAGAAGAAGAGAGGAAGAGGAGCGAAAGAGACAGAGAGGGAGATCAGCTGTCGCTCTTGACGCTTCTTGTGGCTGCTTTTAGGAAGTCTTTGATTGGGTGCAGTACTACTGGGACTGGGGCCGAGAGAGCTAAGCTTTCCAACATGGAGATTGGGTGGCCTTCCAATGTGAGGCATGTTGCCCATGTCACCTTTGATCGGTTCAATGGCTTTCTGGGTTTGCCGGTTGAGCTTGAGCCTGAAGTTCCCAGGAGGGCTCCTAGTGCTAGTGCGAATGTTTTCGGGGTTTCCACAGAGTCCATGCAGCTATCATTTGATGCTAGAGGAAACAGTGTCCCCACAATACTCATCTTGATGCAGAGACATTTATATGCGCAGGGGGGTCTGCAGGCAGAAGGAATCTTCAGAATTAATGGTGAGAACAGTCAGGAAGAGTATGTCAGGGACCAATTAAACAGGGGAATAATACCAGAGGGTGTTGACATGCACTGTTTAGCAGGTCTTATTAAGGCTTGGTTTAGAGAACTCCCAACTGCTGTATTAGACTCTCTCTCACCAGAGCAGGTAATGCAGTCGCAGACAGAAGAGGAGTGTGCTCAACTTGTTAGGCTCCTTCCGCCAACAGAAGCTGCATTATTGGATTGGGCAGTAAACCTAATGGCCGATGTTGCACAGATGGAACACCTGAACAAGATGAATGCACGCAACATTGCCATGGTCTTTGCACCAAACATGACTCAAATGGTAGATCCTTTGACTGCATTGATGTATGCAGTCCAAGTGATGAATTTCCTCAAGACTCTTATAGTCAAGACACTAAGAGAGAGAGAAGAGTCTATGGTGGAAACAGCTCCTGTCCCACGCCTTGAGCCTTCTGATGAGGATGAGCACCAAAGCACTTTTCAACCATATCTCGAAGAGAATAATGAGGAGGCTAACAAGGAAAATGAAAAGGAGAATGTATTTGTTGCCGAAGAACCTGCTTTACAGAGCCCCCCTCGCACTTTACAAGATGAACCTACAGCTGAAAGTGGATCCCAGAATTTCCTCTCTTCTATCAAATATATGATTCCCGGAGGAAATTGGTCTGTAGTTGATAATTGTCCTTGTGAGGTTGTATCCCAAGTCAGCTCTTTAACAAATGGGCTCCAAGAGGATGGCATAACAGGTCAGGGTAGAGAGGCTCAGACAAACGTTTGGAAGAGTCAACCAAGCGTTTCAAATCTGAAGAAGGGATCAAAGGTCACTGAGCAGCTAATGGTTCAAATTGTCGGGCCTGGAGACAAGAGCAAGAGAACTGGGATTCTTAGCCGCATAAATTCGAGGACAGATATCGCTGAAGCGTCGCCGGTGCGCTGGAGAAGACGGGGCTCTACGTCGGAGCTTGAAGGTGGTAGTGGTGGAGTTGGGTTCTTCGACGTCTCCACCGATTCACCGAATCAACGCAGAGCCTTTTTCAGAATGAATATTCATGGCCTCCAAAGCCATTTTTGCTCCTCTGTTCCTTCAACTATGTTACCTGTTCAACTCCCAACTCGAATGGAGAGTCATTTGTGGTATGTTTTACCTGAGGAGGTCAAGAGTGAAACCCTTTTGAAACGGTATTTTGAGCTTCTCTCACCTTGTGAGAAGACAAATGTGCTGCGAATGCGCGGGGATGAGCTCCGAAAGAGAGCCCTTCTGGCCCGTGCGTTGGTTCGGACTACCATTGCTAGATATACAAACGATAGAGTTGATCCGAGGTCATTGAACTTTAAAAAGAACGGTTATGGGAAGCCTGAGGTAGACTGGCAAATTGCAGATGACTGGCAGCCACCTGCACTGCAATTCAACATCTCACACACTTCTTCCTTGATAGCTTGTGGAGTAGCTGTCGATTCACCAATCGGTATCGATGTGGAAGAGAAACAGCGAAAGTTGAAGCACCATATTATAGCTTTTGCCAGACGATACTTCTCTACTCATGAAGTGGAACATTTAACTTCTATCTCAGACATTGAAATTCAGAGGCAACAGTTCCTTCAACTGTGGACTCTCAAGGAGGCATATGTGAAAGCATTGGGGAGGGGCTTCTCTGCTTCACCTTTTAAGACCTTTACCATTAGATTGAAGGCTGCAGCTAAGAGAGGCATTCCTCTATCTGAGGAATTGGATTCTAAGATATCCGAAATAAGTGTCGAGTCTCTTGGCCCTGAAGATCTTACAAAGAACTGGCAGTTTTTGCTTCTAGATTTGGCTGGTTCCCATTATGGTGCCATTTGCATGGAAAAACTTAACACTTTAGGAAAAGGAAATGTTCCCATTAAATTGACAGTGCGAAGAACGATCCCACTTGTTGAAGATGAATGTGTTATAGGAACTGATGCTGTTGTACCAATAGTTGGATTGACTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009628 GO:0034059 GO:0036293 GO:0050896 GO:0070482
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

856

Amino Acids

95.41

Weight (kDa)

5.29

Isoelectric Point (pI)

59.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 170 - 303 3.8e-22 RhoGAP domain
AASDHPPT_N PF22624 590 - 676 6.2e-07 4'-phosphopantetheinyl transferase, N-terminal
ACPS PF01648 685 - 765 8.2e-16 4'-phosphopantetheinyl transferase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011795)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11490
fragaria_vesca FvH4_6g19150
malus_domestica MD12G1027300.v1.1 MD14G1027500.v1.1
prunus_persica Prupe.7G104200_v2.0.a1
pyrus_communis pycom12g02620 pycom14g02620
rosa_chinensis RchiOBHm_Chr3g0474261
rosa_laevigata RLG00000023935
rosa_multiflora Rmu_sc0001803.1_g000025 Rmu_sc0004507.1_g000020 Rmu_sc0029669.1_g000001
rosa_roxburghii Rroxscaffold_6G00407400
rosa_rugosa Rorug03G0139800
rosa_samantha Rh3AG190400 Rh3BG219600 Rh3CG214800 Rh3DG215100
rosa_wichuraiana Rw3G017370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1996
AatII GACGTC 1 cut(s) 1593
Acc16I TGCGCA 1 cut(s) 546
Acc36I ACCTGC 4 cut(s) 659, 724, 1159, 1996
AccB1I GGYRCC 1 cut(s) 2423
AccB7I CCANNNNNTGG 1 cut(s) 2552
AccI GTMKAC 1 cut(s) 1956
AccII CGCG 1 cut(s) 1824
AciI CCGC 3 cut(s) 788, 1483, 1824
AclI AACGTT 1 cut(s) 1365
AclWI GGATC 6 cut(s) 914, 1200, 1213, 1414, 1901, 2491
AcoI YGGCCR 1 cut(s) 831
AcsI RAATTY 4 cut(s) 958, 1213, 1489, 2172
AcuI CTGAAG 5 cut(s) 432, 559, 1418, 1530, 2385
AcyI GRCGYC 1 cut(s) 1590
AdeI CACNNNGTG 1 cut(s) 1796
AfaI GTAC 3 cut(s) 12, 277, 2544
AfiI CCNNNNNNNGG 5 cut(s) 110, 342, 369, 1864, 2552
AjnI CCWGG 2 cut(s) 419, 1447
AjuI GAANNNNNNNTTGG 2 cut(s) 1056, 1088
Alw21I GWGCWC 3 cut(s) 769, 1062, 1836
Alw26I GTCTC 5 cut(s) 195, 526, 1446, 1597, 2358
AlwI GGATC 6 cut(s) 914, 1200, 1213, 1414, 1901, 2491
AlwNI CAGNNNCTG 2 cut(s) 800, 1025
AoxI GGCC 8 cut(s) 73, 291, 329, 831, 1445, 1645, 1854, 2359
ApeKI GCWGC 8 cut(s) 245, 475, 800, 1423, 1813, 1981, 2285, 2288
ApoI RAATTY 4 cut(s) 958, 1213, 1489, 2172
ArsI GACNNNNNNTTYG 4 cut(s) 1702, 1734, 2331, 2363
AseI ATTAAT 1 cut(s) 582
Asp700I GAANNNNTTC 1 cut(s) 449
AspLEI GCGC 3 cut(s) 547, 1527, 1824
AspS9I GGNCC 6 cut(s) 74, 291, 616, 1445, 1855, 2360
AsuC2I CCSGG 1 cut(s) 1245
AsuHPI GGTGA 9 cut(s) 30, 125, 352, 599, 717, 1596, 1782, 2043, 2244
AvaII GGWCC 1 cut(s) 616
AxyI CCTNAGG 2 cut(s) 1740, 1950
BamHI GGATCC 1 cut(s) 1205
BanI GGYRCC 1 cut(s) 2423
BanII GRGCYC 6 cut(s) 430, 1166, 1320, 1544, 1836, 1849
BbsI GAAGAC 2 cut(s) 1540, 1808
Bbv12I GWGCWC 3 cut(s) 769, 1062, 1836
BbvI GCAGC 8 cut(s) 232, 487, 787, 1435, 1800, 1993, 2272, 2300
BccI CCATC 4 cut(s) 31, 65, 841, 1322
BcgI CGANNNNNNTGC 2 cut(s) 1133, 1167
BciT130I CCWGG 2 cut(s) 421, 1449
BciVI GTATCC 1 cut(s) 1300
BcnI CCSGG 1 cut(s) 1245
BcoDI GTCTC 5 cut(s) 195, 526, 1446, 1597, 2358
BfaI CTAG 5 cut(s) 432, 438, 492, 1884, 2399
BfmI CTRYAG 4 cut(s) 557, 1191, 1260, 2286
BfuAI ACCTGC 4 cut(s) 659, 724, 1159, 1996
BfuI GTATCC 1 cut(s) 1300
BglI GCCNNNNNGGC 1 cut(s) 1853
BglII AGATCT 1 cut(s) 2368
BisI GCNGC 9 cut(s) 246, 476, 801, 1424, 1483, 1814, 1982, 2286, 2289
BlpI GCTNAGC 1 cut(s) 303
BlsI GCNGC 9 cut(s) 247, 477, 802, 1425, 1484, 1815, 1983, 2287, 2290
BmcAI AGTACT 1 cut(s) 277
Bme1390I CCNGG 3 cut(s) 421, 1245, 1449
Bme18I GGWCC 1 cut(s) 616
BmgT120I GGNCC 6 cut(s) 74, 291, 616, 1445, 1855, 2360
BmiI GGNNCC 8 cut(s) 292, 429, 617, 782, 1207, 1319, 2413, 2425
BmrFI CCNGG 3 cut(s) 421, 1245, 1449
BmrI ACTGGG 3 cut(s) 291, 297, 1479
BmsI GCATC 3 cut(s) 478, 516, 2524
BmuI ACTGGG 3 cut(s) 291, 297, 1479
BpiI GAAGAC 2 cut(s) 1540, 1808
BplI GAGNNNNNCTC 4 cut(s) 1153, 1185, 1772, 1804
BpmI CTGGAG 2 cut(s) 1470, 1549
Bpu1102I GCTNAGC 1 cut(s) 303
BpuEI CTTGAG 4 cut(s) 425, 950, 1058, 2192
BpuMI CCSGG 1 cut(s) 1245
Bsa29I ATCGAT 1 cut(s) 2064
BsaHI GRCGYC 1 cut(s) 1590
BsaJI CCNNGG 3 cut(s) 419, 885, 1910
BsaXI ACNNNNNCTCC 4 cut(s) 311, 341, 2028, 2058
Bsc4I CCNNNNNNNGG 5 cut(s) 110, 342, 369, 1864, 2552
Bse118I RCCGGY 2 cut(s) 394, 1519
Bse1I ACTGG 7 cut(s) 99, 286, 292, 1474, 1964, 1982, 2387
Bse21I CCTNAGG 2 cut(s) 1740, 1950
Bse3DI GCAATG 2 cut(s) 879, 1878
BseBI CCWGG 2 cut(s) 421, 1449
BseCI ATCGAT 1 cut(s) 2064
BseDI CCNNGG 3 cut(s) 419, 885, 1910
BseGI GGATG 3 cut(s) 1060, 1333, 1834
BseLI CCNNNNNNNGG 5 cut(s) 110, 342, 369, 1864, 2552
BseMI GCAATG 2 cut(s) 879, 1878
BseMII CTCAG 6 cut(s) 1370, 1410, 1731, 1941, 2175, 2304
BseNI ACTGG 7 cut(s) 99, 286, 292, 1474, 1964, 1982, 2387
BseRI GAGGAG 6 cut(s) 158, 205, 773, 1115, 1657, 1757
BseXI GCAGC 8 cut(s) 232, 487, 787, 1435, 1800, 1993, 2272, 2300
BsgI GTGCAG 2 cut(s) 292, 1974
Bsh1236I CGCG 1 cut(s) 1824
BshFI GGCC 8 cut(s) 75, 293, 331, 833, 1447, 1647, 1856, 2361
BshNI GGYRCC 1 cut(s) 2423
BshVI ATCGAT 1 cut(s) 2064
BsiHKAI GWGCWC 3 cut(s) 769, 1062, 1836
BsiSI CCGG 3 cut(s) 395, 1245, 1520
BslFI GGGAC 4 cut(s) 298, 491, 629, 1013
BslI CCNNNNNNNGG 5 cut(s) 110, 342, 369, 1864, 2552
BsmAI GTCTC 5 cut(s) 195, 526, 1446, 1597, 2358
BsmBI CGTCTC 1 cut(s) 1597
BsmFI GGGAC 4 cut(s) 298, 491, 629, 1013
BsmI GAATGC 3 cut(s) 874, 1824, 2301
BsnI GGCC 8 cut(s) 75, 293, 331, 833, 1447, 1647, 1856, 2361
Bsp1286I GDGCHC 8 cut(s) 430, 769, 1062, 1166, 1320, 1544, 1836, 1849
Bsp143I GATC 8 cut(s) 214, 367, 919, 1205, 1406, 1906, 2368, 2496
Bsp1720I GCTNAGC 1 cut(s) 303
Bsp19I CCATGG 1 cut(s) 885
BspACI CCGC 3 cut(s) 788, 1483, 1824
BspANI GGCC 8 cut(s) 75, 293, 331, 833, 1447, 1647, 1856, 2361
BspCNI CTCAG 6 cut(s) 1369, 1411, 1732, 1942, 2174, 2305
BspDI ATCGAT 1 cut(s) 2064
BspFNI CGCG 1 cut(s) 1824
BspHI TCATGA 1 cut(s) 2134
BspLI GGNNCC 8 cut(s) 292, 429, 617, 782, 1207, 1319, 2413, 2425
BspMAI CTGCAG 2 cut(s) 561, 2290
BspMI ACCTGC 4 cut(s) 659, 724, 1159, 1996
BspPI GGATC 6 cut(s) 914, 1200, 1213, 1414, 1901, 2491
BspQI GCTCTTC 1 cut(s) 55
BspT107I GGYRCC 1 cut(s) 2423
BsrDI GCAATG 2 cut(s) 879, 1878
BsrFI RCCGGY 2 cut(s) 394, 1519
BsrI ACTGG 7 cut(s) 99, 286, 292, 1474, 1964, 1982, 2387
BssAI RCCGGY 2 cut(s) 394, 1519
BssECI CCNNGG 3 cut(s) 419, 885, 1910
BssMI GATC 8 cut(s) 214, 367, 919, 1205, 1406, 1906, 2368, 2496
BssNI GRCGYC 1 cut(s) 1590
BssT1I CCWWGG 1 cut(s) 885
Bst2UI CCWGG 2 cut(s) 421, 1449
Bst4CI ACNGT 8 cut(s) 503, 596, 662, 1773, 1937, 2187, 2199, 2485
BstACI GRCGYC 1 cut(s) 1590
BstAPI GCANNNNNTGC 2 cut(s) 739, 2392
BstC8I GCNNGC 4 cut(s) 122, 126, 561, 874
BstDSI CCRYGG 1 cut(s) 885
BstENI CCTNNNNNAGG 1 cut(s) 108
BstF5I GGATG 3 cut(s) 1060, 1333, 1834
BstFNI CGCG 1 cut(s) 1824
BstHHI GCGC 3 cut(s) 547, 1527, 1824
BstKTI GATC 8 cut(s) 217, 370, 922, 1208, 1409, 1909, 2371, 2499
BstMAI GTCTC 5 cut(s) 195, 526, 1446, 1597, 2358
BstMBI GATC 8 cut(s) 214, 367, 919, 1205, 1406, 1906, 2368, 2496
BstNI CCWGG 2 cut(s) 421, 1449
BstNSI RCATGY 3 cut(s) 89, 350, 658
BstSCI CCNGG 3 cut(s) 419, 1243, 1447
BstSFI CTRYAG 4 cut(s) 557, 1191, 1260, 2286
BstUI CGCG 1 cut(s) 1824
BstV1I GCAGC 8 cut(s) 232, 487, 787, 1435, 1800, 1993, 2272, 2300
BstV2I GAAGAC 2 cut(s) 1540, 1808
BstX2I RGATCY 3 cut(s) 919, 1205, 2368
BstXI CCANNNNNNTGG 2 cut(s) 1328, 2434
BstYI RGATCY 3 cut(s) 919, 1205, 2368
Bsu15I ATCGAT 1 cut(s) 2064
Bsu36I CCTNAGG 2 cut(s) 1740, 1950
BsuI GTATCC 1 cut(s) 1300
BsuRI GGCC 8 cut(s) 75, 293, 331, 833, 1447, 1647, 1856, 2361
BsuTUI ATCGAT 1 cut(s) 2064
BtgI CCRYGG 1 cut(s) 885
BtsCI GGATG 3 cut(s) 1060, 1333, 1834
BtsI GCAGTG 1 cut(s) 1991
BtsIMutI CAGTG 5 cut(s) 508, 658, 1416, 1991, 2490
BveI ACCTGC 4 cut(s) 659, 724, 1159, 1996
Cac8I GCNNGC 4 cut(s) 122, 126, 561, 874
CaiI CAGNNNCTG 2 cut(s) 800, 1025
CciI TCATGA 1 cut(s) 2134
CfoI GCGC 3 cut(s) 547, 1527, 1824
Cfr10I RCCGGY 2 cut(s) 394, 1519
Cfr13I GGNCC 6 cut(s) 74, 291, 616, 1445, 1855, 2360
ClaI ATCGAT 1 cut(s) 2064
CseI GACGC 2 cut(s) 241, 1503
Csp6I GTAC 3 cut(s) 11, 276, 2543
CviQI GTAC 3 cut(s) 11, 276, 2543
DpnI GATC 8 cut(s) 216, 369, 921, 1207, 1408, 1908, 2370, 2498
DpnII GATC 8 cut(s) 214, 367, 919, 1205, 1406, 1906, 2368, 2496
DraI TTTAAA 1 cut(s) 1927
DraIII CACNNNGTG 1 cut(s) 1796
EaeI YGGCCR 1 cut(s) 831
EciI GGCGGA 1 cut(s) 777
Ecl136II GAGCTC 1 cut(s) 1834
Eco130I CCWWGG 1 cut(s) 885
Eco24I GRGCYC 6 cut(s) 430, 1166, 1320, 1544, 1836, 1849
Eco32I GATATC 2 cut(s) 1504, 2334
Eco47I GGWCC 1 cut(s) 616
Eco53kI GAGCTC 1 cut(s) 1834
Eco57I CTGAAG 5 cut(s) 432, 559, 1418, 1530, 2385
Eco81I CCTNAGG 2 cut(s) 1740, 1950
EcoICRI GAGCTC 1 cut(s) 1834
EcoNI CCTNNNNNAGG 1 cut(s) 108
EcoRII CCWGG 2 cut(s) 419, 1447
EcoRV GATATC 2 cut(s) 1504, 2334
EcoT14I CCWWGG 1 cut(s) 885
EcoT38I GRGCYC 6 cut(s) 430, 1166, 1320, 1544, 1836, 1849
ErhI CCWWGG 1 cut(s) 885
Esp3I CGTCTC 1 cut(s) 1597
FaqI GGGAC 4 cut(s) 298, 491, 629, 1013
FauI CCCGC 1 cut(s) 1817
FauNDI CATATG 1 cut(s) 2218
FblI GTMKAC 1 cut(s) 1956
Fnu4HI GCNGC 9 cut(s) 246, 476, 801, 1424, 1483, 1814, 1982, 2286, 2289
FokI GGATG 3 cut(s) 1067, 1340, 1841
FriOI GRGCYC 6 cut(s) 430, 1166, 1320, 1544, 1836, 1849
Fsp4HI GCNGC 9 cut(s) 246, 476, 801, 1424, 1483, 1814, 1982, 2286, 2289
FspBI CTAG 5 cut(s) 432, 438, 492, 1884, 2399
FspI TGCGCA 1 cut(s) 546
GlaI GCGC 3 cut(s) 546, 1526, 1823
GluI GCNGC 9 cut(s) 246, 476, 801, 1424, 1483, 1814, 1982, 2286, 2289
GsuI CTGGAG 2 cut(s) 1470, 1549
HaeIII GGCC 8 cut(s) 75, 293, 331, 833, 1447, 1647, 1856, 2361
HapII CCGG 3 cut(s) 395, 1245, 1520
HgaI GACGC 2 cut(s) 241, 1503
HhaI GCGC 3 cut(s) 547, 1527, 1824
Hin1I GRCGYC 1 cut(s) 1590
Hin6I GCGC 3 cut(s) 545, 1525, 1822
HinP1I GCGC 3 cut(s) 545, 1525, 1822
HincII GTYRAC 2 cut(s) 652, 1379
HindII GTYRAC 2 cut(s) 652, 1379
HindIII AAGCTT 1 cut(s) 305
HpaII CCGG 3 cut(s) 395, 1245, 1520
HphI GGTGA 9 cut(s) 30, 125, 352, 599, 717, 1596, 1782, 2043, 2244
Hpy166II GTNNAC 5 cut(s) 652, 823, 1379, 1957, 2202
Hpy188III TCNNGA 9 cut(s) 229, 523, 599, 967, 982, 1085, 1750, 2135, 2399
Hpy8I GTNNAC 5 cut(s) 652, 823, 1379, 1957, 2202
Hpy99I CGWCG 3 cut(s) 1519, 1552, 1592
HpyCH4III ACNGT 8 cut(s) 503, 596, 662, 1773, 1937, 2187, 2199, 2485
HpyCH4IV ACGT 3 cut(s) 1365, 1547, 1590
HpySE526I ACGT 3 cut(s) 1365, 1547, 1590
Hsp92I GRCGYC 1 cut(s) 1590
HspAI GCGC 3 cut(s) 545, 1525, 1822
Kzo9I GATC 8 cut(s) 214, 367, 919, 1205, 1406, 1906, 2368, 2496
LguI GCTCTTC 1 cut(s) 55
LmnI GCTCC 8 cut(s) 192, 433, 786, 1027, 1323, 1552, 1670, 1839
Lsp1109I GCAGC 8 cut(s) 232, 487, 787, 1435, 1800, 1993, 2272, 2300
LweI GCATC 3 cut(s) 478, 516, 2524
MaeI CTAG 5 cut(s) 432, 438, 492, 1884, 2399
MaeII ACGT 3 cut(s) 1365, 1547, 1590
MaeIII GTNAC 3 cut(s) 358, 1414, 1686
MalI GATC 8 cut(s) 216, 369, 921, 1207, 1408, 1908, 2370, 2498
MboI GATC 8 cut(s) 214, 367, 919, 1205, 1406, 1906, 2368, 2496
MflI RGATCY 3 cut(s) 919, 1205, 2368
MhlI GDGCHC 8 cut(s) 430, 769, 1062, 1166, 1320, 1544, 1836, 1849
MlyI GAGTC 9 cut(s) 476, 710, 901, 964, 1013, 1384, 1726, 2197, 2360
MmeI TCCRAC 3 cut(s) 336, 1530, 2533
MroXI GAANNNNTTC 1 cut(s) 449
MseI TTAA 9 cut(s) 582, 623, 678, 1307, 1926, 2150, 2259, 2445, 2475
MslI CAYNNNNRTG 5 cut(s) 524, 540, 884, 2421, 2432
MspA1I CMGCKG 2 cut(s) 220, 1196
MspI CCGG 3 cut(s) 395, 1245, 1520
MspR9I CCNGG 3 cut(s) 421, 1245, 1449
Mva1269I GAATGC 3 cut(s) 874, 1824, 2301
MvaI CCWGG 2 cut(s) 421, 1449
MvnI CGCG 1 cut(s) 1824
NciI CCSGG 1 cut(s) 1245
NcoI CCATGG 1 cut(s) 885
NdeI CATATG 1 cut(s) 2218
NdeII GATC 8 cut(s) 214, 367, 919, 1205, 1406, 1906, 2368, 2496
NlaIV GGNNCC 8 cut(s) 292, 429, 617, 782, 1207, 1319, 2413, 2425
NmeAIII GCCGAG 1 cut(s) 319
NmuCI GTSAC 2 cut(s) 358, 1414
NsbI TGCGCA 1 cut(s) 546
NspI RCATGY 3 cut(s) 89, 350, 658
PagI TCATGA 1 cut(s) 2134
PaqCI CACCTGC 1 cut(s) 1996
PciSI GCTCTTC 1 cut(s) 55
PcsI WCGNNNNNNNCGW 1 cut(s) 1596
PctI GAATGC 3 cut(s) 874, 1824, 2301
PdmI GAANNNNTTC 1 cut(s) 449
PfeI GAWTC 7 cut(s) 570, 1240, 1474, 1601, 1609, 2048, 2324
PflMI CCANNNNNTGG 1 cut(s) 2552
PfoI TCCNGGA 1 cut(s) 1243
PkrI GCNGC 9 cut(s) 247, 477, 802, 1425, 1484, 1815, 1983, 2287, 2290
PleI GAGTC 9 cut(s) 475, 710, 901, 964, 1012, 1383, 1725, 2197, 2359
PpsI GAGTC 9 cut(s) 475, 710, 901, 964, 1012, 1383, 1725, 2197, 2359
PshBI ATTAAT 1 cut(s) 582
Psp124BI GAGCTC 1 cut(s) 1836
Psp1406I AACGTT 1 cut(s) 1365
Psp6I CCWGG 2 cut(s) 419, 1447
PspGI CCWGG 2 cut(s) 419, 1447
PspN4I GGNNCC 8 cut(s) 292, 429, 617, 782, 1207, 1319, 2413, 2425
PspPI GGNCC 6 cut(s) 74, 291, 616, 1445, 1855, 2360
PsrI GAACNNNNNNTAC 2 cut(s) 1140, 1172
PstI CTGCAG 2 cut(s) 561, 2290
PstNI CAGNNNCTG 2 cut(s) 800, 1025
PsuI RGATCY 3 cut(s) 919, 1205, 2368
PvuII CAGCTG 2 cut(s) 220, 1196
RsaI GTAC 3 cut(s) 12, 277, 2544
RsaNI GTAC 3 cut(s) 11, 276, 2543
RseI CAYNNNNRTG 5 cut(s) 524, 540, 884, 2421, 2432
SacI GAGCTC 1 cut(s) 1836
SapI GCTCTTC 1 cut(s) 55
SaqAI TTAA 9 cut(s) 582, 623, 678, 1307, 1926, 2150, 2259, 2445, 2475
SatI GCNGC 9 cut(s) 246, 476, 801, 1424, 1483, 1814, 1982, 2286, 2289
Sau3AI GATC 8 cut(s) 214, 367, 919, 1205, 1406, 1906, 2368, 2496
Sau96I GGNCC 6 cut(s) 74, 291, 616, 1445, 1855, 2360
ScaI AGTACT 1 cut(s) 277
SchI GAGTC 9 cut(s) 476, 710, 901, 964, 1013, 1384, 1726, 2197, 2360
ScrFI CCNGG 3 cut(s) 421, 1245, 1449
SduI GDGCHC 8 cut(s) 430, 769, 1062, 1166, 1320, 1544, 1836, 1849
SfaNI GCATC 3 cut(s) 478, 516, 2524
SfcI CTRYAG 4 cut(s) 557, 1191, 1260, 2286
SgrAI CRCCGGYG 1 cut(s) 1519
SinI GGWCC 1 cut(s) 616
SmiMI CAYNNNNRTG 5 cut(s) 524, 540, 884, 2421, 2432
SmlI CTYRAG 4 cut(s) 404, 965, 1037, 2207
SmoI CTYRAG 4 cut(s) 404, 965, 1037, 2207
SsiI CCGC 3 cut(s) 788, 1483, 1824
SspI AATATT 1 cut(s) 1639
SspMI CTAG 5 cut(s) 432, 438, 492, 1884, 2399
SstI GAGCTC 1 cut(s) 1836
StyD4I CCNGG 3 cut(s) 419, 1243, 1447
StyI CCWWGG 1 cut(s) 885
TaaI ACNGT 8 cut(s) 503, 596, 662, 1773, 1937, 2187, 2199, 2485
TaiI ACGT 3 cut(s) 1368, 1550, 1593
TaqI TCGA 7 cut(s) 1086, 1493, 1587, 1708, 2046, 2064, 2349
TatI WGTACW 1 cut(s) 275
TauI GCSGC 1 cut(s) 1485
TfiI GAWTC 7 cut(s) 570, 1240, 1474, 1601, 1609, 2048, 2324
Tru1I TTAA 9 cut(s) 582, 623, 678, 1307, 1926, 2150, 2259, 2445, 2475
Tru9I TTAA 9 cut(s) 582, 623, 678, 1307, 1926, 2150, 2259, 2445, 2475
TscAI CASTG 5 cut(s) 508, 665, 1423, 1998, 2490
TseFI GTSAC 2 cut(s) 358, 1414
TseI GCWGC 8 cut(s) 245, 475, 800, 1423, 1813, 1981, 2285, 2288
Tsp45I GTSAC 2 cut(s) 358, 1414
TspDTI ATGAA 8 cut(s) 881, 971, 1134, 1200, 1631, 1649, 2151, 2529
TspGWI ACGGA 1 cut(s) 97
TspRI CASTG 5 cut(s) 508, 665, 1423, 1998, 2490
Van91I CCANNNNNTGG 1 cut(s) 2552
VpaK11BI GGWCC 1 cut(s) 616
VspI ATTAAT 1 cut(s) 582
XagI CCTNNNNNAGG 1 cut(s) 108
XapI RAATTY 4 cut(s) 958, 1213, 1489, 2172
XbaI TCTAGA 1 cut(s) 2398
XceI RCATGY 3 cut(s) 89, 350, 658
XmiI GTMKAC 1 cut(s) 1956
XmnI GAANNNNTTC 1 cut(s) 449
XspI CTAG 5 cut(s) 432, 438, 492, 1884, 2399
ZraI GACGTC 1 cut(s) 1591
ZrmI AGTACT 1 cut(s) 277
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.