MD12G1027300.v1.1

rho GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0011795
Physical Location & Seq
Reverse (-)
3069088 .. 3074031
4944 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1027300.v1.1.491

Sequence Viewer

Length: 1551 bp
ATGACCGAGGTCCTCCAATCACCGTCCACATCTCACTTCCCTTCCCCTCCAAGCTCCTCCACTCCATGTGCTGCTGCTCCACCTACACCCAATGACTCACCATACCCACATGCCCTTTTAGAGGATACCCTCCAGGGGTATTTGGCTTCGGAGGAAGACGAAGAAAACGAAAGCGAAGACGAAGAGGAAGAAGAGAGGAAGAAGAAGGAGAGGGACGGAGAGGGTGATCAGCTTTCCCTTGTGACCCTTTTGGTTACTGCTTTCAGGAGGTCTTTGATTGGGTGTAGCAGTACTAATAGTGCAGATAGTGGAAGAGGGAAGCTTTCTTCCATGGAGATTGGGTGGCCTTCGAATGTCAGGCATGTTGCCCATGTCACCTTTGATCGGTTCAATGGGTTTCTTGGTTTGCCTGTTGAGCTTGAACCTGAGGTCCCCAGGAGGGCTCCGAGTGCCAGCGCAAATGTTTTTGGGGTCTCAACAGAGTCTATGCAGCTTTCTTTTGATGCCAGAGGGAACAGTGTCCCAACAATACTTATTTTGATGCAGAGACATTTGTATGCACAAGGTGGTCTGCAGGCTGAAGGGATCTTCAGAATTAATGCTGAGAACAGTCAGGAGGAGTACGTCAGGGACCAATTAAATCGGGGAGTGATACCAGAGGGCGTTGACGTGCATTGTTTGGCCGGTCTTATTAAGGCTTGGTTCCGAGAACTTCCAACTGCTGTGTTAGACTCTCTAACACCAGAGCAGGTAATGCAGTCCCAGTCGGAAGAGGAGTGTGCTGAACTTGTTAGGCTCCTACCTCCAACAGAAGCTGCGTTATTGGATTGGGCAGTAAACCTAATGGCTGATGTTGCACAAATGGAACACTTCAACAAGATGAATGCACGCAATATTGCCATGGTGTTTGCACCAAACATGACTCATATGGTAGATCCTTTGACTGCATTGATGTATGCCGTCCAAGTGATGAATTTCCTCAAGACTCTTATTGTCAAGACACTAAAAGAAAGAGAGGAATCTTTGGTGGAAACAGCCCCTGTACCCCGCCTAGAGCCTTCTGACGAGGATGAGCACCAAAGCACTTTTCAACCATATCACAAAGAGGCCAACGAGGAGGCTAACAAGGAAAATGAAGAGGAGGGCGTATTTGTTGGCGAAGAACCTGATTTAGAGAGCCCCCTTCATTCTACACAAAATGATCCCATAACCGAAAGTGGATCTCAAACTTTTCTATCTTCTATCAAGAATATCATTCCGGGAGGAAACCGGTTTCTGGCAGATAATTGTGCTTGCGAGGTTGTATCCCAAGTTAGCTCTTTGGCAAACGGGCCCCAAGATCAGGGTTTAACAGGTTCAGGTAGAGAGGCTCAGCCAAACGTTAGGAAGATCAAAAGTGGTCAATCAAGCGGTTCGAATCTGAAGAAGGGGCCCAAGAAGGTAAACGAGCAGCCACTGATTCAAACTGCTGTGACCGCAGATAAGAGCAAAAGAAGTGAAATTCTTAGCCGCATAAATTCGAGGACGGAGTTGGCTGAAGGTTGGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0008150 GO:0009628 GO:0034059 GO:0036293 GO:0050896 GO:0070482
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PBD RhoGAP

Protein Analysis

517

Amino Acids

56.75

Weight (kDa)

4.75

Isoelectric Point (pI)

61.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RhoGAP PF00620 175 - 309 1.4e-22 RhoGAP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011795)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G11490
fragaria_vesca FvH4_6g19150
malus_domestica MD12G1027300.v1.1 MD14G1027500.v1.1
prunus_persica Prupe.7G104200_v2.0.a1
pyrus_communis pycom12g02620 pycom14g02620
rosa_chinensis RchiOBHm_Chr3g0474261
rosa_laevigata RLG00000023935
rosa_multiflora Rmu_sc0001803.1_g000025 Rmu_sc0004507.1_g000020 Rmu_sc0029669.1_g000001
rosa_roxburghii Rroxscaffold_6G00407400
rosa_rugosa Rorug03G0139800
rosa_samantha Rh3AG190400 Rh3BG219600 Rh3CG214800 Rh3DG215100
rosa_wichuraiana Rw3G017370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 739
AciI CCGC 4 cut(s) 1048, 1410, 1476, 1510
AclI AACGTT 1 cut(s) 1380
AclWI GGATC 4 cut(s) 593, 929, 1196, 1228
AcoI YGGCCR 1 cut(s) 681
AcsI RAATTY 3 cut(s) 973, 1500, 1516
AcuI CTGAAG 3 cut(s) 574, 600, 1442
AdeI CACNNNGTG 1 cut(s) 566
AfaI GTAC 3 cut(s) 292, 623, 1044
AfiI CCNNNNNNNGG 6 cut(s) 121, 135, 384, 439, 1276, 1342
AgeI ACCGGT 1 cut(s) 1269
AgsI TTSAA 5 cut(s) 391, 422, 874, 1091, 1463
AjiI CACGTC 1 cut(s) 670
AjnI CCWGG 2 cut(s) 132, 434
AjuI GAANNNNNNNTTGG 2 cut(s) 1071, 1103
AloI GAACNNNNNNTCC 2 cut(s) 414, 446
AluBI AGCT 7 cut(s) 54, 232, 322, 418, 493, 815, 1317
AluI AGCT 7 cut(s) 54, 232, 322, 418, 493, 815, 1317
Alw21I GWGCWC 1 cut(s) 1077
Alw26I GTCTC 2 cut(s) 478, 541
AlwI GGATC 4 cut(s) 593, 929, 1196, 1228
AlwNI CAGNNNCTG 3 cut(s) 815, 1040, 1456
AoxI GGCC 5 cut(s) 344, 681, 1107, 1331, 1430
ApaI GGGCCC 2 cut(s) 1335, 1434
ApeKI GCWGC 5 cut(s) 71, 74, 490, 815, 1450
ApoI RAATTY 3 cut(s) 973, 1500, 1516
AseI ATTAAT 1 cut(s) 597
AsiGI ACCGGT 1 cut(s) 1269
AspLEI GCGC 1 cut(s) 458
AspS9I GGNCC 7 cut(s) 10, 430, 631, 1331, 1332, 1430, 1431
AsuC2I CCSGG 1 cut(s) 1260
AsuHPI GGTGA 4 cut(s) 12, 90, 236, 367
AsuII TTCGAA 2 cut(s) 350, 1415
AvaII GGWCC 3 cut(s) 10, 430, 631
AxyI CCTNAGG 1 cut(s) 426
BaeGI GKGCMC 2 cut(s) 1335, 1434
BanII GRGCYC 4 cut(s) 445, 1181, 1335, 1434
BbsI GAAGAC 2 cut(s) 162, 183
Bbv12I GWGCWC 1 cut(s) 1077
BbvI GCAGC 5 cut(s) 58, 61, 502, 802, 1462
BceAI ACGGC 1 cut(s) 944
BciT130I CCWGG 2 cut(s) 134, 436
BciVI GTATCC 2 cut(s) 118, 1315
BclI TGATCA 1 cut(s) 226
BcnI CCSGG 1 cut(s) 1260
BcoDI GTCTC 2 cut(s) 478, 541
BfaI CTAG 1 cut(s) 1052
BfmI CTRYAG 1 cut(s) 572
BfuAI ACCTGC 1 cut(s) 739
BfuI GTATCC 2 cut(s) 118, 1315
BisI GCNGC 6 cut(s) 72, 75, 491, 816, 1451, 1510
BlpI GCTNAGC 1 cut(s) 1371
BlsI GCNGC 6 cut(s) 73, 76, 492, 817, 1452, 1511
BmcAI AGTACT 1 cut(s) 292
Bme1390I CCNGG 3 cut(s) 134, 436, 1260
Bme18I GGWCC 3 cut(s) 10, 430, 631
BmgBI CACGTC 1 cut(s) 670
BmgT120I GGNCC 7 cut(s) 10, 430, 631, 1331, 1332, 1430, 1431
BmiI GGNNCC 9 cut(s) 432, 444, 632, 704, 797, 1333, 1334, 1431, 1432
BmrFI CCNGG 3 cut(s) 134, 436, 1260
BmrI ACTGGG 1 cut(s) 757
BmsI GCATC 2 cut(s) 493, 531
BmuI ACTGGG 1 cut(s) 757
BoxI GACNNNNGTC 1 cut(s) 8
BpiI GAAGAC 2 cut(s) 162, 183
BpmI CTGGAG 1 cut(s) 116
Bpu1102I GCTNAGC 1 cut(s) 1371
Bpu14I TTCGAA 2 cut(s) 350, 1415
BpuEI CTTGAG 1 cut(s) 965
BpuMI CCSGG 1 cut(s) 1260
BsaI GGTCTC 1 cut(s) 478
BsaJI CCNNGG 5 cut(s) 6, 133, 330, 434, 900
BsaWI WCCGGW 1 cut(s) 1269
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 6 cut(s) 121, 135, 384, 439, 1276, 1342
Bse118I RCCGGY 2 cut(s) 683, 1269
Bse1I ACTGG 1 cut(s) 763
Bse21I CCTNAGG 1 cut(s) 426
BseBI CCWGG 2 cut(s) 134, 436
BseDI CCNNGG 5 cut(s) 6, 133, 330, 434, 900
BseGI GGATG 1 cut(s) 1075
BseLI CCNNNNNNNGG 6 cut(s) 121, 135, 384, 439, 1276, 1342
BseMII CTCAG 3 cut(s) 417, 594, 1385
BseNI ACTGG 1 cut(s) 763
BseRI GAGGAG 5 cut(s) 46, 632, 788, 1130, 1154
BseSI GKGCMC 2 cut(s) 1335, 1434
BseXI GCAGC 5 cut(s) 58, 61, 502, 802, 1462
BsgI GTGCAG 1 cut(s) 321
BshFI GGCC 5 cut(s) 346, 683, 1109, 1333, 1432
BshTI ACCGGT 1 cut(s) 1269
BsiHKAI GWGCWC 1 cut(s) 1077
BsiSI CCGG 3 cut(s) 684, 1259, 1270
BslFI GGGAC 5 cut(s) 227, 416, 506, 644, 745
BslI CCNNNNNNNGG 6 cut(s) 121, 135, 384, 439, 1276, 1342
BsmAI GTCTC 2 cut(s) 478, 541
BsmFI GGGAC 5 cut(s) 227, 416, 506, 644, 745
BsmI GAATGC 1 cut(s) 889
BsnI GGCC 5 cut(s) 346, 683, 1109, 1333, 1432
Bso31I GGTCTC 1 cut(s) 478
Bsp119I TTCGAA 2 cut(s) 350, 1415
Bsp120I GGGCCC 2 cut(s) 1331, 1430
Bsp1286I GDGCHC 5 cut(s) 445, 1077, 1181, 1335, 1434
Bsp143I GATC 8 cut(s) 226, 382, 585, 934, 1201, 1220, 1339, 1389
Bsp1720I GCTNAGC 1 cut(s) 1371
Bsp19I CCATGG 2 cut(s) 330, 900
BspACI CCGC 4 cut(s) 1048, 1410, 1476, 1510
BspANI GGCC 5 cut(s) 346, 683, 1109, 1333, 1432
BspCNI CTCAG 3 cut(s) 418, 595, 1384
BspLI GGNNCC 9 cut(s) 432, 444, 632, 704, 797, 1333, 1334, 1431, 1432
BspMAI CTGCAG 1 cut(s) 576
BspMI ACCTGC 1 cut(s) 739
BspPI GGATC 4 cut(s) 593, 929, 1196, 1228
BspT104I TTCGAA 2 cut(s) 350, 1415
BspTNI GGTCTC 1 cut(s) 478
BsrFI RCCGGY 2 cut(s) 683, 1269
BsrI ACTGG 1 cut(s) 763
BssAI RCCGGY 2 cut(s) 683, 1269
BssECI CCNNGG 5 cut(s) 6, 133, 330, 434, 900
BssMI GATC 8 cut(s) 226, 382, 585, 934, 1201, 1220, 1339, 1389
BssT1I CCWWGG 2 cut(s) 330, 900
Bst2UI CCWGG 2 cut(s) 134, 436
Bst4CI ACNGT 3 cut(s) 24, 518, 611
Bst6I CTCTTC 5 cut(s) 177, 186, 307, 765, 1131
BstAPI GCANNNNNTGC 1 cut(s) 754
BstBI TTCGAA 2 cut(s) 350, 1415
BstC8I GCNNGC 4 cut(s) 454, 576, 889, 1294
BstDEI CTNAG 4 cut(s) 426, 603, 1371, 1505
BstDSI CCRYGG 2 cut(s) 330, 900
BstENI CCTNNNNNAGG 1 cut(s) 119
BstF5I GGATG 1 cut(s) 1075
BstHHI GCGC 1 cut(s) 458
BstKTI GATC 8 cut(s) 229, 385, 588, 937, 1204, 1223, 1342, 1392
BstMAI GTCTC 2 cut(s) 478, 541
BstMBI GATC 8 cut(s) 226, 382, 585, 934, 1201, 1220, 1339, 1389
BstMWI GCNNNNNNNGC 5 cut(s) 415, 449, 754, 854, 1475
BstNI CCWGG 2 cut(s) 134, 436
BstNSI RCATGY 2 cut(s) 113, 365
BstPAI GACNNNNGTC 1 cut(s) 8
BstSCI CCNGG 3 cut(s) 132, 434, 1258
BstSFI CTRYAG 1 cut(s) 572
BstSLI GKGCMC 2 cut(s) 1335, 1434
BstV1I GCAGC 5 cut(s) 58, 61, 502, 802, 1462
BstV2I GAAGAC 2 cut(s) 162, 183
BstX2I RGATCY 3 cut(s) 585, 934, 1220
BstYI RGATCY 3 cut(s) 585, 934, 1220
Bsu36I CCTNAGG 1 cut(s) 426
BsuI GTATCC 2 cut(s) 118, 1315
BsuRI GGCC 5 cut(s) 346, 683, 1109, 1333, 1432
BtgI CCRYGG 2 cut(s) 330, 900
BtrI CACGTC 1 cut(s) 670
BtsCI GGATG 1 cut(s) 1075
BtsIMutI CAGTG 2 cut(s) 523, 1454
BveI ACCTGC 1 cut(s) 739
Cac8I GCNNGC 4 cut(s) 454, 576, 889, 1294
CaiI CAGNNNCTG 3 cut(s) 815, 1040, 1456
CfoI GCGC 1 cut(s) 458
Cfr10I RCCGGY 2 cut(s) 683, 1269
Cfr13I GGNCC 7 cut(s) 10, 430, 631, 1331, 1332, 1430, 1431
Csp6I GTAC 3 cut(s) 291, 622, 1043
CspAI ACCGGT 1 cut(s) 1269
CviAII CATG 7 cut(s) 66, 110, 331, 362, 371, 901, 919
CviQI GTAC 3 cut(s) 291, 622, 1043
DdeI CTNAG 4 cut(s) 426, 603, 1371, 1505
DpnI GATC 8 cut(s) 228, 384, 587, 936, 1203, 1222, 1341, 1391
DpnII GATC 8 cut(s) 226, 382, 585, 934, 1201, 1220, 1339, 1389
DraIII CACNNNGTG 1 cut(s) 566
EaeI YGGCCR 1 cut(s) 681
Eam1104I CTCTTC 5 cut(s) 177, 186, 307, 765, 1131
EarI CTCTTC 5 cut(s) 177, 186, 307, 765, 1131
Eco130I CCWWGG 2 cut(s) 330, 900
Eco24I GRGCYC 4 cut(s) 445, 1181, 1335, 1434
Eco31I GGTCTC 1 cut(s) 478
Eco47I GGWCC 3 cut(s) 10, 430, 631
Eco57I CTGAAG 3 cut(s) 574, 600, 1442
Eco81I CCTNAGG 1 cut(s) 426
EcoNI CCTNNNNNAGG 1 cut(s) 119
EcoO109I RGGNCCY 4 cut(s) 10, 430, 1332, 1430
EcoRII CCWGG 2 cut(s) 132, 434
EcoT14I CCWWGG 2 cut(s) 330, 900
EcoT38I GRGCYC 4 cut(s) 445, 1181, 1335, 1434
ErhI CCWWGG 2 cut(s) 330, 900
FaeI CATG 7 cut(s) 69, 113, 334, 365, 374, 904, 922
FaqI GGGAC 5 cut(s) 227, 416, 506, 644, 745
FatI CATG 7 cut(s) 65, 109, 330, 361, 370, 900, 918
FauI CCCGC 1 cut(s) 1055
FauNDI CATATG 1 cut(s) 927
FbaI TGATCA 1 cut(s) 226
Fnu4HI GCNGC 6 cut(s) 72, 75, 491, 816, 1451, 1510
FokI GGATG 1 cut(s) 1082
FriOI GRGCYC 4 cut(s) 445, 1181, 1335, 1434
Fsp4HI GCNGC 6 cut(s) 72, 75, 491, 816, 1451, 1510
FspBI CTAG 1 cut(s) 1052
GlaI GCGC 1 cut(s) 457
GluI GCNGC 6 cut(s) 72, 75, 491, 816, 1451, 1510
GsuI CTGGAG 1 cut(s) 116
HaeIII GGCC 5 cut(s) 346, 683, 1109, 1333, 1432
HapII CCGG 3 cut(s) 684, 1259, 1270
HhaI GCGC 1 cut(s) 458
Hin1II CATG 7 cut(s) 69, 113, 334, 365, 374, 904, 922
Hin6I GCGC 1 cut(s) 456
HinP1I GCGC 1 cut(s) 456
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 8 cut(s) 95, 482, 731, 922, 985, 1019, 1417, 1459
HpaII CCGG 3 cut(s) 684, 1259, 1270
HphI GGTGA 4 cut(s) 12, 90, 236, 367
Hpy166II GTNNAC 4 cut(s) 27, 667, 838, 1444
Hpy188I TCNGA 7 cut(s) 151, 447, 593, 707, 769, 1063, 1422
Hpy188III TCNNGA 5 cut(s) 265, 614, 982, 997, 1246
Hpy8I GTNNAC 4 cut(s) 27, 667, 838, 1444
HpyAV CCTTC 9 cut(s) 51, 199, 357, 575, 1068, 1193, 1420, 1432, 1532
HpyCH4III ACNGT 3 cut(s) 24, 518, 611
HpyCH4IV ACGT 3 cut(s) 624, 669, 1380
HpyF10VI GCNNNNNNNGC 5 cut(s) 415, 449, 754, 854, 1475
HpyF3I CTNAG 4 cut(s) 426, 603, 1371, 1505
HpySE526I ACGT 3 cut(s) 624, 669, 1380
Hsp92II CATG 7 cut(s) 69, 113, 334, 365, 374, 904, 922
HspAI GCGC 1 cut(s) 456
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 8 cut(s) 226, 382, 585, 934, 1201, 1220, 1339, 1389
LmnI GCTCC 4 cut(s) 59, 82, 448, 801
Lsp1109I GCAGC 5 cut(s) 58, 61, 502, 802, 1462
LweI GCATC 2 cut(s) 493, 531
MaeI CTAG 1 cut(s) 1052
MaeII ACGT 3 cut(s) 624, 669, 1380
MaeIII GTNAC 4 cut(s) 241, 253, 373, 1471
MalI GATC 8 cut(s) 228, 384, 587, 936, 1203, 1222, 1341, 1391
MboI GATC 8 cut(s) 226, 382, 585, 934, 1201, 1220, 1339, 1389
MflI RGATCY 3 cut(s) 585, 934, 1220
MhlI GDGCHC 5 cut(s) 445, 1077, 1181, 1335, 1434
MluCI AATT 6 cut(s) 594, 635, 973, 1285, 1500, 1516
MlyI GAGTC 5 cut(s) 89, 491, 725, 916, 979
MmeI TCCRAC 3 cut(s) 740, 747, 830
MseI TTAA 4 cut(s) 597, 638, 693, 1349
MslI CAYNNNNRTG 1 cut(s) 555
MspI CCGG 3 cut(s) 684, 1259, 1270
MspR9I CCNGG 3 cut(s) 134, 436, 1260
Mva1269I GAATGC 1 cut(s) 889
MvaI CCWGG 2 cut(s) 134, 436
MwoI GCNNNNNNNGC 5 cut(s) 415, 449, 754, 854, 1475
NciI CCSGG 1 cut(s) 1260
NcoI CCATGG 2 cut(s) 330, 900
NdeI CATATG 1 cut(s) 927
NdeII GATC 8 cut(s) 226, 382, 585, 934, 1201, 1220, 1339, 1389
NlaIII CATG 7 cut(s) 69, 113, 334, 365, 374, 904, 922
NlaIV GGNNCC 9 cut(s) 432, 444, 632, 704, 797, 1333, 1334, 1431, 1432
NmuCI GTSAC 3 cut(s) 241, 373, 1471
NspI RCATGY 2 cut(s) 113, 365
NspV TTCGAA 2 cut(s) 350, 1415
PcsI WCGNNNNNNNCGW 1 cut(s) 165
PctI GAATGC 1 cut(s) 889
PfeI GAWTC 3 cut(s) 1019, 1417, 1459
PfoI TCCNGGA 1 cut(s) 1258
PinAI ACCGGT 1 cut(s) 1269
PkrI GCNGC 6 cut(s) 73, 76, 492, 817, 1452, 1511
PleI GAGTC 5 cut(s) 89, 490, 725, 916, 979
PpsI GAGTC 5 cut(s) 89, 490, 725, 916, 979
PpuMI RGGWCCY 2 cut(s) 10, 430
PshAI GACNNNNGTC 1 cut(s) 8
PshBI ATTAAT 1 cut(s) 597
Psp1406I AACGTT 1 cut(s) 1380
Psp5II RGGWCCY 2 cut(s) 10, 430
Psp6I CCWGG 2 cut(s) 132, 434
PspGI CCWGG 2 cut(s) 132, 434
PspN4I GGNNCC 9 cut(s) 432, 444, 632, 704, 797, 1333, 1334, 1431, 1432
PspOMI GGGCCC 2 cut(s) 1331, 1430
PspPI GGNCC 7 cut(s) 10, 430, 631, 1331, 1332, 1430, 1431
PspPPI RGGWCCY 2 cut(s) 10, 430
PstI CTGCAG 1 cut(s) 576
PstNI CAGNNNCTG 3 cut(s) 815, 1040, 1456
PsuI RGATCY 3 cut(s) 585, 934, 1220
RsaI GTAC 3 cut(s) 292, 623, 1044
RsaNI GTAC 3 cut(s) 291, 622, 1043
RseI CAYNNNNRTG 1 cut(s) 555
SaqAI TTAA 4 cut(s) 597, 638, 693, 1349
SatI GCNGC 6 cut(s) 72, 75, 491, 816, 1451, 1510
Sau3AI GATC 8 cut(s) 226, 382, 585, 934, 1201, 1220, 1339, 1389
Sau96I GGNCC 7 cut(s) 10, 430, 631, 1331, 1332, 1430, 1431
ScaI AGTACT 1 cut(s) 292
SchI GAGTC 5 cut(s) 89, 491, 725, 916, 979
ScrFI CCNGG 3 cut(s) 134, 436, 1260
SduI GDGCHC 5 cut(s) 445, 1077, 1181, 1335, 1434
SfaNI GCATC 2 cut(s) 493, 531
SfcI CTRYAG 1 cut(s) 572
SfuI TTCGAA 2 cut(s) 350, 1415
SinI GGWCC 3 cut(s) 10, 430, 631
SmiMI CAYNNNNRTG 1 cut(s) 555
SmlI CTYRAG 1 cut(s) 980
SmoI CTYRAG 1 cut(s) 980
Sse9I AATT 6 cut(s) 594, 635, 973, 1285, 1500, 1516
SsiI CCGC 4 cut(s) 1048, 1410, 1476, 1510
SspI AATATT 1 cut(s) 895
SspMI CTAG 1 cut(s) 1052
StyD4I CCNGG 3 cut(s) 132, 434, 1258
StyI CCWWGG 2 cut(s) 330, 900
TaaI ACNGT 3 cut(s) 24, 518, 611
TaiI ACGT 3 cut(s) 627, 672, 1383
TaqI TCGA 3 cut(s) 350, 1415, 1520
TaqII GACCGA 1 cut(s) 20
TasI AATT 6 cut(s) 594, 635, 973, 1285, 1500, 1516
TatI WGTACW 1 cut(s) 290
TauI GCSGC 1 cut(s) 1512
TfiI GAWTC 3 cut(s) 1019, 1417, 1459
Tru1I TTAA 4 cut(s) 597, 638, 693, 1349
Tru9I TTAA 4 cut(s) 597, 638, 693, 1349
TscAI CASTG 2 cut(s) 523, 1461
TseFI GTSAC 3 cut(s) 241, 373, 1471
TseI GCWGC 5 cut(s) 71, 74, 490, 815, 1450
Tsp45I GTSAC 3 cut(s) 241, 373, 1471
TspDTI ATGAA 4 cut(s) 896, 986, 1149, 1175
TspGWI ACGGA 2 cut(s) 231, 1541
TspRI CASTG 2 cut(s) 523, 1461
VpaK11BI GGWCC 3 cut(s) 10, 430, 631
VspI ATTAAT 1 cut(s) 597
XagI CCTNNNNNAGG 1 cut(s) 119
XapI RAATTY 3 cut(s) 973, 1500, 1516
XceI RCATGY 2 cut(s) 113, 365
XspI CTAG 1 cut(s) 1052
ZrmI AGTACT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.