FvH4_6g19750

Secoisolariciresinol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016366
Physical Location & Seq
Forward (+)
13231489 .. 13232614
1126 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g19750.t1

Sequence Viewer

Length: 858 bp
ATGAGCATGAGTTCTTCCCCACCAGCTCCTCATGGTCCCAAGAGGTTAGAAGGCAAAGTAGCAATCATCACCGGGGGTGCCAGCGGAATCGGAGAGAGCACCGCAAGACTGTTTGTTTATCACGGCGCCAAAGTCATCATCGCCGATGTCCAAGACGAGCTTGCTCTATCCCTCTGCAAAGAACTCGACCCGGACCAAGAATCCATTCCGTACGTCCATTGCGATGTCACAATCGATTCCGATGTGAAAAATGTTGTAGATGTGGCAATCTCCAACTACGGAAAACTCGATATCATGTACAACAATGCTGGCATACCCGGGAACTTGGACCCGACGATTTTAGGTGCTGATCACCAGAACTTCAAGCAAGTGTTTGATGTGAATGTTTACGGGGCTTTCTTGGGCGCCAAGCATGCTGCTAGGGTTATGATCCCTGCGAAAAATGGCAGCATTCTCTTCACTTCGAGTGTGACATCGGCTAGTTGTGGTGAGTCTTCACATGCCTATACGATGTCGAAGCACGCGGTGGTGGGGCTTATGAAGAGTTTATGTGTGGAGTTGGGGCAGTATGGTATTAGAGTTAATTGCATTTCTCCATGCGCTATGGCTACTCCATTGTTAACAAATGCCATGGGTTTGGAGAAGAATGTGGTGGAGGAATTGATTTGTGCTTCGGCTGTATTGAAAGGAGTTGTGCCCAGATCAGAGGATGTAGCCGATGCTGCAGTGTTCTTGGCCAGCGAGGAGTCGAAGTTTGTCGGTGGACTAAACCTTCTTGTTGATGGGGGTTATAGCACTACCAACCAATCGTTCAGTAGGGTTCTGAGGAATGTGATGTCCTCAAACCAAGTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

286

Amino Acids

30.07

Weight (kDa)

5.47

Isoelectric Point (pI)

31.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 19 - 209 1.5e-49 short chain dehydrogenase
SDR PF23441 19 - 263 8.3e-09 SDR-like rossmann domain
KR PF08659 22 - 175 1.8e-08 KR domain
adh_short_C2 PF13561 27 - 265 5e-61 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016366)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g19750
malus_domestica MD12G1032700.v1.1 MD14G1033600.v1.1
prunus_persica Prupe.7G099400_v2.0.a1
pyrus_communis pycom12g03020 pycom14g02970
rosa_chinensis RchiOBHm_Chr3g0475091
rosa_laevigata RLG00000023863
rosa_multiflora Rmu_sc0003093.1_g000023
rosa_roxburghii Rroxscaffold_6G00406570
rosa_rugosa Rorug03G0146700
rosa_samantha Rh3AG196500 Rh3BG226500 Rh3DG221900
rosa_wichuraiana Rw3G017880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 77, 125, 404
AccII CGCG 1 cut(s) 524
AciI CCGC 3 cut(s) 84, 102, 524
AclWI GGATC 1 cut(s) 424
AcoI YGGCCR 1 cut(s) 735
AcyI GRCGYC 2 cut(s) 126, 405
AdeI CACNNNGTG 1 cut(s) 526
AfaI GTAC 2 cut(s) 212, 299
AgsI TTSAA 2 cut(s) 364, 685
AjuI GAANNNNNNNTTGG 4 cut(s) 189, 221, 794, 826
AluBI AGCT 2 cut(s) 26, 160
AluI AGCT 2 cut(s) 26, 160
Alw21I GWGCWC 1 cut(s) 101
AlwI GGATC 1 cut(s) 424
Ama87I CYCGRG 1 cut(s) 317
AoxI GGCC 1 cut(s) 735
ApeKI GCWGC 3 cut(s) 416, 447, 722
Asp700I GAANNNNTTC 1 cut(s) 204
AspLEI GCGC 3 cut(s) 128, 407, 602
AspS9I GGNCC 3 cut(s) 35, 193, 328
AsuC2I CCSGG 4 cut(s) 73, 191, 318, 319
AsuHPI GGTGA 3 cut(s) 61, 344, 500
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 3 cut(s) 35, 193, 328
BaeGI GKGCMC 1 cut(s) 699
BalI TGGCCA 1 cut(s) 737
BanI GGYRCC 3 cut(s) 77, 125, 404
BbsI GAAGAC 1 cut(s) 486
Bbv12I GWGCWC 1 cut(s) 101
BbvI GCAGC 3 cut(s) 403, 459, 709
BccI CCATC 1 cut(s) 776
BceAI ACGGC 1 cut(s) 139
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BclI TGATCA 1 cut(s) 349
BcnI CCSGG 4 cut(s) 73, 191, 318, 319
BfaI CTAG 2 cut(s) 420, 480
BfmI CTRYAG 1 cut(s) 723
BfoI RGCGCY 2 cut(s) 129, 408
BisI GCNGC 3 cut(s) 417, 448, 723
BlsI GCNGC 3 cut(s) 418, 449, 724
Bme1390I CCNGG 4 cut(s) 73, 191, 318, 319
Bme18I GGWCC 3 cut(s) 35, 193, 328
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 3 cut(s) 35, 193, 328
BmiI GGNNCC 5 cut(s) 37, 79, 127, 330, 406
BmrFI CCNGG 4 cut(s) 73, 191, 318, 319
BmsI GCATC 1 cut(s) 709
BpiI GAAGAC 1 cut(s) 486
BpuMI CCSGG 4 cut(s) 73, 191, 318, 319
Bsa29I ATCGAT 1 cut(s) 234
BsaHI GRCGYC 2 cut(s) 126, 405
BsaJI CCNNGG 3 cut(s) 72, 317, 630
BsaXI ACNNNNNCTCC 2 cut(s) 737, 767
Bse3DI GCAATG 1 cut(s) 217
BseCI ATCGAT 1 cut(s) 234
BseDI CCNNGG 3 cut(s) 72, 317, 630
BseGI GGATG 1 cut(s) 715
BseMI GCAATG 1 cut(s) 217
BseMII CTCAG 1 cut(s) 815
BseRI GAGGAG 2 cut(s) 18, 758
BseSI GKGCMC 1 cut(s) 699
BseXI GCAGC 3 cut(s) 403, 459, 709
Bsh1236I CGCG 1 cut(s) 524
BshFI GGCC 1 cut(s) 737
BshNI GGYRCC 3 cut(s) 77, 125, 404
BshVI ATCGAT 1 cut(s) 234
BsiHKAI GWGCWC 1 cut(s) 101
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 3 cut(s) 72, 191, 318
BsiWI CGTACG 1 cut(s) 210
BslFI GGGAC 1 cut(s) 21
BsmFI GGGAC 1 cut(s) 21
BsmI GAATGC 1 cut(s) 450
BsnI GGCC 1 cut(s) 737
BsoBI CYCGRG 1 cut(s) 317
Bsp1286I GDGCHC 2 cut(s) 101, 699
Bsp1407I TGTACA 1 cut(s) 297
Bsp143I GATC 3 cut(s) 349, 429, 701
Bsp19I CCATGG 1 cut(s) 630
BspACI CCGC 3 cut(s) 84, 102, 524
BspANI GGCC 1 cut(s) 737
BspCNI CTCAG 1 cut(s) 816
BspDI ATCGAT 1 cut(s) 234
BspFNI CGCG 1 cut(s) 524
BspLI GGNNCC 5 cut(s) 37, 79, 127, 330, 406
BspMAI CTGCAG 1 cut(s) 727
BspPI GGATC 1 cut(s) 424
BspT107I GGYRCC 3 cut(s) 77, 125, 404
BsrDI GCAATG 1 cut(s) 217
BsrGI TGTACA 1 cut(s) 297
BssECI CCNNGG 3 cut(s) 72, 317, 630
BssMI GATC 3 cut(s) 349, 429, 701
BssNI GRCGYC 2 cut(s) 126, 405
BssT1I CCWWGG 1 cut(s) 630
Bst4CI ACNGT 1 cut(s) 111
Bst6I CTCTTC 2 cut(s) 461, 536
BstACI GRCGYC 2 cut(s) 126, 405
BstAUI TGTACA 1 cut(s) 297
BstC8I GCNNGC 6 cut(s) 82, 162, 310, 414, 522, 739
BstDEI CTNAG 1 cut(s) 824
BstDSI CCRYGG 1 cut(s) 630
BstF5I GGATG 1 cut(s) 715
BstFNI CGCG 1 cut(s) 524
BstH2I RGCGCY 2 cut(s) 129, 408
BstHHI GCGC 3 cut(s) 128, 407, 602
BstKTI GATC 3 cut(s) 352, 432, 704
BstMBI GATC 3 cut(s) 349, 429, 701
BstMWI GCNNNNNNNGC 2 cut(s) 413, 722
BstNSI RCATGY 2 cut(s) 416, 503
BstSCI CCNGG 4 cut(s) 71, 189, 316, 317
BstSFI CTRYAG 1 cut(s) 723
BstSLI GKGCMC 1 cut(s) 699
BstUI CGCG 1 cut(s) 524
BstV1I GCAGC 3 cut(s) 403, 459, 709
BstV2I GAAGAC 1 cut(s) 486
BstXI CCANNNNNNTGG 1 cut(s) 637
Bsu15I ATCGAT 1 cut(s) 234
BsuRI GGCC 1 cut(s) 737
BsuTUI ATCGAT 1 cut(s) 234
BtgI CCRYGG 1 cut(s) 630
BtgZI GCGATG 2 cut(s) 124, 237
BtsCI GGATG 1 cut(s) 715
BtsI GCAGTG 1 cut(s) 732
BtsIMutI CAGTG 1 cut(s) 732
Cac8I GCNNGC 6 cut(s) 82, 162, 310, 414, 522, 739
CfoI GCGC 3 cut(s) 128, 407, 602
Cfr13I GGNCC 3 cut(s) 35, 193, 328
Cfr9I CCCGGG 1 cut(s) 317
ClaI ATCGAT 1 cut(s) 234
Csp6I GTAC 2 cut(s) 211, 298
CviAII CATG 7 cut(s) 7, 32, 295, 413, 500, 597, 631
CviJI RGCY 9 cut(s) 26, 160, 395, 479, 535, 608, 677, 716, 737
CviKI_1 RGCY 9 cut(s) 26, 160, 395, 479, 535, 608, 677, 716, 737
CviQI GTAC 2 cut(s) 211, 298
DdeI CTNAG 1 cut(s) 824
DinI GGCGCC 2 cut(s) 127, 406
DpnI GATC 3 cut(s) 351, 431, 703
DpnII GATC 3 cut(s) 349, 429, 701
DraIII CACNNNGTG 1 cut(s) 526
EaeI YGGCCR 1 cut(s) 735
Eam1104I CTCTTC 2 cut(s) 461, 536
EarI CTCTTC 2 cut(s) 461, 536
Eco130I CCWWGG 1 cut(s) 630
Eco32I GATATC 1 cut(s) 292
Eco47I GGWCC 3 cut(s) 35, 193, 328
Eco88I CYCGRG 1 cut(s) 317
EcoRV GATATC 1 cut(s) 292
EcoT14I CCWWGG 1 cut(s) 630
EgeI GGCGCC 2 cut(s) 127, 406
EheI GGCGCC 2 cut(s) 127, 406
ErhI CCWWGG 1 cut(s) 630
FaeI CATG 7 cut(s) 10, 35, 298, 416, 503, 600, 634
FalI AAGNNNNNCTT 2 cut(s) 144, 176
FaqI GGGAC 1 cut(s) 21
FatI CATG 7 cut(s) 6, 31, 294, 412, 499, 596, 630
FbaI TGATCA 1 cut(s) 349
Fnu4HI GCNGC 3 cut(s) 417, 448, 723
FokI GGATG 1 cut(s) 722
Fsp4HI GCNGC 3 cut(s) 417, 448, 723
FspBI CTAG 2 cut(s) 420, 480
GlaI GCGC 3 cut(s) 127, 406, 601
GluI GCNGC 3 cut(s) 417, 448, 723
HaeII RGCGCY 2 cut(s) 129, 408
HaeIII GGCC 1 cut(s) 737
HapII CCGG 3 cut(s) 72, 191, 318
HhaI GCGC 3 cut(s) 128, 407, 602
Hin1I GRCGYC 2 cut(s) 126, 405
Hin1II CATG 7 cut(s) 10, 35, 298, 416, 503, 600, 634
Hin6I GCGC 3 cut(s) 126, 405, 600
HinP1I GCGC 3 cut(s) 126, 405, 600
HincII GTYRAC 1 cut(s) 621
HindII GTYRAC 1 cut(s) 621
HinfI GANTC 5 cut(s) 87, 200, 236, 491, 746
HpaI GTTAAC 1 cut(s) 621
HpaII CCGG 3 cut(s) 72, 191, 318
HphI GGTGA 3 cut(s) 61, 344, 500
Hpy166II GTNNAC 3 cut(s) 388, 621, 764
Hpy188I TCNGA 4 cut(s) 92, 241, 706, 825
Hpy8I GTNNAC 3 cut(s) 388, 621, 764
Hpy99I CGWCG 1 cut(s) 337
HpyAV CCTTC 2 cut(s) 44, 782
HpyCH4III ACNGT 1 cut(s) 111
HpyCH4IV ACGT 1 cut(s) 213
HpyCH4V TGCA 3 cut(s) 177, 588, 725
HpyF10VI GCNNNNNNNGC 2 cut(s) 413, 722
HpyF3I CTNAG 1 cut(s) 824
HpySE526I ACGT 1 cut(s) 213
Hsp92I GRCGYC 2 cut(s) 126, 405
Hsp92II CATG 7 cut(s) 10, 35, 298, 416, 503, 600, 634
HspAI GCGC 3 cut(s) 126, 405, 600
KasI GGCGCC 2 cut(s) 125, 404
Ksp22I TGATCA 1 cut(s) 349
KspAI GTTAAC 1 cut(s) 621
Kzo9I GATC 3 cut(s) 349, 429, 701
LmnI GCTCC 1 cut(s) 31
Lsp1109I GCAGC 3 cut(s) 403, 459, 709
LweI GCATC 1 cut(s) 709
MaeI CTAG 2 cut(s) 420, 480
MaeII ACGT 1 cut(s) 213
MaeIII GTNAC 2 cut(s) 226, 469
MalI GATC 3 cut(s) 351, 431, 703
MboI GATC 3 cut(s) 349, 429, 701
MboII GAAGA 5 cut(s) 6, 448, 486, 553, 655
MhlI GDGCHC 2 cut(s) 101, 699
MlsI TGGCCA 1 cut(s) 737
MluCI AATT 2 cut(s) 583, 659
MluNI TGGCCA 1 cut(s) 737
Mly113I GGCGCC 2 cut(s) 126, 405
MlyI GAGTC 2 cut(s) 500, 755
MmeI TCCRAC 1 cut(s) 297
MnlI CCTC 8 cut(s) 36, 39, 182, 649, 700, 736, 819, 850
Mox20I TGGCCA 1 cut(s) 737
MroXI GAANNNNTTC 1 cut(s) 204
MscI TGGCCA 1 cut(s) 737
MseI TTAA 2 cut(s) 582, 620
MslI CAYNNNNRTG 1 cut(s) 222
Msp20I TGGCCA 1 cut(s) 737
MspA1I CMGCKG 1 cut(s) 84
MspI CCGG 3 cut(s) 72, 191, 318
MspR9I CCNGG 4 cut(s) 73, 191, 318, 319
Mva1269I GAATGC 1 cut(s) 450
MvnI CGCG 1 cut(s) 524
MwoI GCNNNNNNNGC 2 cut(s) 413, 722
NarI GGCGCC 2 cut(s) 126, 405
NciI CCSGG 4 cut(s) 73, 191, 318, 319
NcoI CCATGG 1 cut(s) 630
NdeII GATC 3 cut(s) 349, 429, 701
NlaIII CATG 7 cut(s) 10, 35, 298, 416, 503, 600, 634
NlaIV GGNNCC 5 cut(s) 37, 79, 127, 330, 406
NmuCI GTSAC 2 cut(s) 226, 469
NspI RCATGY 2 cut(s) 416, 503
PaeI GCATGC 1 cut(s) 416
PcsI WCGNNNNNNNCGW 2 cut(s) 219, 285
PctI GAATGC 1 cut(s) 450
PdmI GAANNNNTTC 1 cut(s) 204
PfeI GAWTC 3 cut(s) 87, 200, 236
Pfl23II CGTACG 1 cut(s) 210
PkrI GCNGC 3 cut(s) 418, 449, 724
PleI GAGTC 2 cut(s) 499, 754
PluTI GGCGCC 2 cut(s) 129, 408
PpsI GAGTC 2 cut(s) 499, 754
PspLI CGTACG 1 cut(s) 210
PspN4I GGNNCC 5 cut(s) 37, 79, 127, 330, 406
PspPI GGNCC 3 cut(s) 35, 193, 328
PstI CTGCAG 1 cut(s) 727
RsaI GTAC 2 cut(s) 212, 299
RsaNI GTAC 2 cut(s) 211, 298
RseI CAYNNNNRTG 1 cut(s) 222
SaqAI TTAA 2 cut(s) 582, 620
SatI GCNGC 3 cut(s) 417, 448, 723
Sau3AI GATC 3 cut(s) 349, 429, 701
Sau96I GGNCC 3 cut(s) 35, 193, 328
SchI GAGTC 2 cut(s) 500, 755
ScrFI CCNGG 4 cut(s) 73, 191, 318, 319
SduI GDGCHC 2 cut(s) 101, 699
SetI ASST 6 cut(s) 28, 47, 162, 216, 346, 774
SfaNI GCATC 1 cut(s) 709
SfcI CTRYAG 1 cut(s) 723
SfoI GGCGCC 2 cut(s) 127, 406
SinI GGWCC 3 cut(s) 35, 193, 328
SmaI CCCGGG 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 222
SphI GCATGC 1 cut(s) 416
Sse9I AATT 2 cut(s) 583, 659
SsiI CCGC 3 cut(s) 84, 102, 524
SspDI GGCGCC 2 cut(s) 125, 404
SspMI CTAG 2 cut(s) 420, 480
StyD4I CCNGG 4 cut(s) 71, 189, 316, 317
StyI CCWWGG 1 cut(s) 630
TaaI ACNGT 1 cut(s) 111
TaiI ACGT 1 cut(s) 216
TaqI TCGA 6 cut(s) 186, 234, 288, 464, 515, 749
TasI AATT 2 cut(s) 583, 659
TatI WGTACW 1 cut(s) 297
TfiI GAWTC 3 cut(s) 87, 200, 236
Tru1I TTAA 2 cut(s) 582, 620
Tru9I TTAA 2 cut(s) 582, 620
TscAI CASTG 1 cut(s) 732
TseFI GTSAC 2 cut(s) 226, 469
TseI GCWGC 3 cut(s) 416, 447, 722
Tsp45I GTSAC 2 cut(s) 226, 469
TspDTI ATGAA 1 cut(s) 554
TspGWI ACGGA 2 cut(s) 198, 294
TspMI CCCGGG 1 cut(s) 317
TspRI CASTG 1 cut(s) 732
VpaK11BI GGWCC 3 cut(s) 35, 193, 328
XceI RCATGY 2 cut(s) 416, 503
XmaI CCCGGG 1 cut(s) 317
XmnI GAANNNNTTC 1 cut(s) 204
XspI CTAG 2 cut(s) 420, 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.