pycom12g03020

Secoisolariciresinol dehydrogenase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0016366
Physical Location & Seq
Forward (+)
2729066 .. 2730051
986 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g03020.1

Sequence Viewer

Length: 858 bp
ATGAGCAGTCCTTCCTCACCAGCTCCAATTCCGGGTCCTAAAAGGTTAGAAGGCAAAGTGGCAATTATCACCGGAGGTGCTAGTGGAATTGGAGAGAGCACTGCAAGATTGTTTGTGTTCCATGGTGCAAAAGTCGTCATAGCCGACGTCCAAGACGAACTAGCCCTGTCCCTCTGCAAAGAACTCGACCCAGACGGAGATTCCATTTCGTACATACGTTGCGATGTCACTGTCGATTCGGATGTGAAAAATGTTGTGGACGTGGCTCTGTCGATGTACGGAAGGCTTGACATCATGTACAACAATGCAGGCGTATCCGGCAAATTGGACCCAACTATTTTGGGTGCTGATGGCGAAAACTTCAAGCAAGTGTTTGATGTGAATGTCTATGGTGCTTTCTTGGGCGCCAAGCATGCTGCTAGGGCAATGATCCCTGCTAAAAAAGGTGTCATTCTTTTCACTTCAAGCGTGGCGTCAGTGACTTGCGGCCAGTCTACACACGCGTACGCCATGTCGAAGCACGCGGTGGTGGGACTTATGAAAAGCTTGTGCGTGGACTTGGGGCAGCACGGAATTAGAGTCAATTGCATATCTCCGGGTGCCATGGCAACCCCGTTTCTGACGAATCTTTTGGGGATTGAAAGAAATACGGTGGAGGAGATGATCTGCGCTTCGGCGGTGTTGAAAGGAGTGGTGCCGAGGGCAGAGGATGTGGCGGAGGCTGCCGTGTACTTGGCGAGTGACGAGTCCAAATTTGTGAATGGACTGAACCTTCTTGTGGATGGAGGTTATAGCACCACTAATCAATCTCTTACTAGGGTTTTGAGGGATTTCATGTCATCAAACCAGGTTCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

286

Amino Acids

29.96

Weight (kDa)

5.55

Isoelectric Point (pI)

28.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 19 - 209 3.9e-51 short chain dehydrogenase
SDR PF23441 19 - 263 1.6e-07 SDR-like rossmann domain
KR PF08659 22 - 182 2.9e-11 KR domain
adh_short_C2 PF13561 27 - 265 4.3e-63 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016366)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g19750
malus_domestica MD12G1032700.v1.1 MD14G1033600.v1.1
prunus_persica Prupe.7G099400_v2.0.a1
pyrus_communis pycom12g03020 pycom14g02970
rosa_chinensis RchiOBHm_Chr3g0475091
rosa_laevigata RLG00000023863
rosa_multiflora Rmu_sc0003093.1_g000023
rosa_roxburghii Rroxscaffold_6G00406570
rosa_rugosa Rorug03G0146700
rosa_samantha Rh3AG196500 Rh3BG226500 Rh3DG221900
rosa_wichuraiana Rw3G017880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 150
AccB1I GGYRCC 3 cut(s) 404, 599, 694
AccI GTMKAC 1 cut(s) 494
AccII CGCG 2 cut(s) 503, 524
AciI CCGC 4 cut(s) 486, 524, 677, 716
AclWI GGATC 1 cut(s) 424
AcoI YGGCCR 1 cut(s) 487
AcsI RAATTY 1 cut(s) 752
AcyI GRCGYC 3 cut(s) 147, 405, 473
AdeI CACNNNGTG 1 cut(s) 526
AfaI GTAC 5 cut(s) 212, 278, 299, 506, 731
AfiI CCNNNNNNNGG 2 cut(s) 32, 778
AflIII ACRYGT 1 cut(s) 501
AgsI TTSAA 4 cut(s) 364, 465, 641, 685
AjiI CACGTC 1 cut(s) 262
AjnI CCWGG 1 cut(s) 846
AluBI AGCT 2 cut(s) 23, 546
AluI AGCT 2 cut(s) 23, 546
Alw21I GWGCWC 1 cut(s) 101
AlwI GGATC 1 cut(s) 424
AlwNI CAGNNNCTG 1 cut(s) 853
AoxI GGCC 1 cut(s) 487
ApeKI GCWGC 3 cut(s) 416, 565, 722
ApoI RAATTY 1 cut(s) 752
ArsI GACNNNNNNTTYG 2 cut(s) 613, 645
AspLEI GCGC 2 cut(s) 407, 671
AspS9I GGNCC 2 cut(s) 35, 328
AsuC2I CCSGG 2 cut(s) 33, 597
AsuHPI GGTGA 2 cut(s) 9, 61
AvaII GGWCC 2 cut(s) 35, 328
BanI GGYRCC 3 cut(s) 404, 599, 694
Bbv12I GWGCWC 1 cut(s) 101
BbvI GCAGC 3 cut(s) 403, 577, 709
BccI CCATC 2 cut(s) 344, 776
BceAI ACGGC 1 cut(s) 710
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciT130I CCWGG 1 cut(s) 848
BciVI GTATCC 1 cut(s) 325
BcnI CCSGG 2 cut(s) 33, 597
BfaI CTAG 4 cut(s) 81, 161, 420, 816
BfoI RGCGCY 1 cut(s) 408
BfuI GTATCC 1 cut(s) 325
BisI GCNGC 4 cut(s) 417, 487, 566, 723
BlsI GCNGC 4 cut(s) 418, 488, 567, 724
Bme1390I CCNGG 3 cut(s) 33, 597, 848
Bme18I GGWCC 2 cut(s) 35, 328
BmgBI CACGTC 1 cut(s) 262
BmgT120I GGNCC 2 cut(s) 35, 328
BmiI GGNNCC 5 cut(s) 36, 330, 406, 601, 696
BmrFI CCNGG 3 cut(s) 33, 597, 848
BpuMI CCSGG 2 cut(s) 33, 597
BsaHI GRCGYC 3 cut(s) 147, 405, 473
BsaJI CCNNGG 3 cut(s) 121, 603, 698
BsaWI WCCGGW 1 cut(s) 71
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 778
Bse1I ACTGG 1 cut(s) 490
Bse3DI GCAATG 1 cut(s) 432
BseBI CCWGG 1 cut(s) 848
BseDI CCNNGG 3 cut(s) 121, 603, 698
BseGI GGATG 3 cut(s) 247, 715, 787
BseLI CCNNNNNNNGG 2 cut(s) 32, 778
BseMI GCAATG 1 cut(s) 432
BseNI ACTGG 1 cut(s) 490
BseRI GAGGAG 1 cut(s) 671
BseXI GCAGC 3 cut(s) 403, 577, 709
Bsh1236I CGCG 2 cut(s) 503, 524
BshFI GGCC 1 cut(s) 489
BshNI GGYRCC 3 cut(s) 404, 599, 694
BsiHKAI GWGCWC 1 cut(s) 101
BsiSI CCGG 4 cut(s) 32, 72, 318, 596
BsiWI CGTACG 1 cut(s) 504
BslFI GGGAC 2 cut(s) 154, 546
BslI CCNNNNNNNGG 2 cut(s) 32, 778
BsmFI GGGAC 2 cut(s) 154, 546
BsnI GGCC 1 cut(s) 489
Bsp1286I GDGCHC 1 cut(s) 101
Bsp1407I TGTACA 1 cut(s) 297
Bsp143I GATC 2 cut(s) 429, 663
Bsp19I CCATGG 2 cut(s) 121, 603
BspACI CCGC 4 cut(s) 486, 524, 677, 716
BspANI GGCC 1 cut(s) 489
BspFNI CGCG 2 cut(s) 503, 524
BspLI GGNNCC 5 cut(s) 36, 330, 406, 601, 696
BspPI GGATC 1 cut(s) 424
BspT107I GGYRCC 3 cut(s) 404, 599, 694
BsrDI GCAATG 1 cut(s) 432
BsrGI TGTACA 1 cut(s) 297
BsrI ACTGG 1 cut(s) 490
BssECI CCNNGG 3 cut(s) 121, 603, 698
BssMI GATC 2 cut(s) 429, 663
BssNI GRCGYC 3 cut(s) 147, 405, 473
BssT1I CCWWGG 2 cut(s) 121, 603
Bst2UI CCWGG 1 cut(s) 848
Bst4CI ACNGT 2 cut(s) 232, 652
BstACI GRCGYC 3 cut(s) 147, 405, 473
BstAUI TGTACA 1 cut(s) 297
BstC8I GCNNGC 3 cut(s) 310, 414, 522
BstDSI CCRYGG 2 cut(s) 121, 603
BstF5I GGATG 3 cut(s) 247, 715, 787
BstFNI CGCG 2 cut(s) 503, 524
BstH2I RGCGCY 1 cut(s) 408
BstHHI GCGC 2 cut(s) 407, 671
BstKTI GATC 2 cut(s) 432, 666
BstMBI GATC 2 cut(s) 429, 663
BstMWI GCNNNNNNNGC 4 cut(s) 318, 413, 422, 722
BstNI CCWGG 1 cut(s) 848
BstNSI RCATGY 1 cut(s) 416
BstSCI CCNGG 3 cut(s) 31, 595, 846
BstUI CGCG 2 cut(s) 503, 524
BstV1I GCAGC 3 cut(s) 403, 577, 709
BsuI GTATCC 1 cut(s) 325
BsuRI GGCC 1 cut(s) 489
BtgI CCRYGG 2 cut(s) 121, 603
BtgZI GCGATG 1 cut(s) 237
BtrI CACGTC 1 cut(s) 262
BtsCI GGATG 3 cut(s) 247, 715, 787
BtsI GCAGTG 1 cut(s) 99
BtsIMutI CAGTG 3 cut(s) 99, 228, 483
Cac8I GCNNGC 3 cut(s) 310, 414, 522
CaiI CAGNNNCTG 1 cut(s) 853
CfoI GCGC 2 cut(s) 407, 671
Cfr13I GGNCC 2 cut(s) 35, 328
CseI GACGC 1 cut(s) 462
CsiI ACCWGGT 1 cut(s) 846
Csp6I GTAC 5 cut(s) 211, 277, 298, 505, 730
CviAII CATG 6 cut(s) 122, 295, 413, 511, 604, 835
CviJI RGCY 8 cut(s) 23, 143, 164, 266, 286, 489, 546, 722
CviKI_1 RGCY 8 cut(s) 23, 143, 164, 266, 286, 489, 546, 722
CviQI GTAC 5 cut(s) 211, 277, 298, 505, 730
DinI GGCGCC 1 cut(s) 406
DpnI GATC 2 cut(s) 431, 665
DpnII GATC 2 cut(s) 429, 663
DraIII CACNNNGTG 1 cut(s) 526
EaeI YGGCCR 1 cut(s) 487
EciI GGCGGA 1 cut(s) 731
Eco130I CCWWGG 2 cut(s) 121, 603
Eco47I GGWCC 2 cut(s) 35, 328
EcoO109I RGGNCCY 1 cut(s) 35
EcoRII CCWGG 1 cut(s) 846
EcoT14I CCWWGG 2 cut(s) 121, 603
EgeI GGCGCC 1 cut(s) 406
EheI GGCGCC 1 cut(s) 406
ErhI CCWWGG 2 cut(s) 121, 603
FaeI CATG 6 cut(s) 125, 298, 416, 514, 607, 838
FaqI GGGAC 2 cut(s) 154, 546
FatI CATG 6 cut(s) 121, 294, 412, 510, 603, 834
FblI GTMKAC 1 cut(s) 494
Fnu4HI GCNGC 4 cut(s) 417, 487, 566, 723
FokI GGATG 3 cut(s) 254, 722, 794
Fsp4HI GCNGC 4 cut(s) 417, 487, 566, 723
FspBI CTAG 4 cut(s) 81, 161, 420, 816
GlaI GCGC 2 cut(s) 406, 670
GluI GCNGC 4 cut(s) 417, 487, 566, 723
HaeII RGCGCY 1 cut(s) 408
HaeIII GGCC 1 cut(s) 489
HapII CCGG 4 cut(s) 32, 72, 318, 596
HgaI GACGC 1 cut(s) 462
HhaI GCGC 2 cut(s) 407, 671
Hin1I GRCGYC 3 cut(s) 147, 405, 473
Hin1II CATG 6 cut(s) 125, 298, 416, 514, 607, 838
Hin6I GCGC 2 cut(s) 405, 669
HinP1I GCGC 2 cut(s) 405, 669
HindIII AAGCTT 1 cut(s) 544
HinfI GANTC 5 cut(s) 200, 236, 579, 625, 746
HpaII CCGG 4 cut(s) 32, 72, 318, 596
HphI GGTGA 2 cut(s) 9, 61
Hpy166II GTNNAC 4 cut(s) 259, 495, 556, 730
Hpy188I TCNGA 2 cut(s) 241, 621
Hpy8I GTNNAC 4 cut(s) 259, 495, 556, 730
Hpy99I CGWCG 1 cut(s) 149
HpyAV CCTTC 4 cut(s) 21, 44, 276, 782
HpyCH4III ACNGT 2 cut(s) 232, 652
HpyCH4IV ACGT 3 cut(s) 147, 217, 261
HpyCH4V TGCA 5 cut(s) 104, 128, 177, 308, 588
HpyF10VI GCNNNNNNNGC 4 cut(s) 318, 413, 422, 722
HpySE526I ACGT 3 cut(s) 147, 217, 261
Hsp92I GRCGYC 3 cut(s) 147, 405, 473
Hsp92II CATG 6 cut(s) 125, 298, 416, 514, 607, 838
HspAI GCGC 2 cut(s) 405, 669
KasI GGCGCC 1 cut(s) 404
Kzo9I GATC 2 cut(s) 429, 663
LmnI GCTCC 1 cut(s) 28
Lsp1109I GCAGC 3 cut(s) 403, 577, 709
MabI ACCWGGT 1 cut(s) 846
MaeI CTAG 4 cut(s) 81, 161, 420, 816
MaeII ACGT 3 cut(s) 147, 217, 261
MaeIII GTNAC 3 cut(s) 226, 478, 740
MalI GATC 2 cut(s) 431, 665
MboI GATC 2 cut(s) 429, 663
MfeI CAATTG 1 cut(s) 583
MhlI GDGCHC 1 cut(s) 101
MluCI AATT 7 cut(s) 27, 63, 87, 323, 573, 583, 752
MluI ACGCGT 1 cut(s) 501
Mly113I GGCGCC 1 cut(s) 405
MlyI GAGTC 2 cut(s) 588, 755
MnlI CCTC 9 cut(s) 25, 68, 182, 649, 693, 700, 712, 779, 819
MspI CCGG 4 cut(s) 32, 72, 318, 596
MspR9I CCNGG 3 cut(s) 33, 597, 848
MunI CAATTG 1 cut(s) 583
MvaI CCWGG 1 cut(s) 848
MvnI CGCG 2 cut(s) 503, 524
MwoI GCNNNNNNNGC 4 cut(s) 318, 413, 422, 722
NarI GGCGCC 1 cut(s) 405
NciI CCSGG 2 cut(s) 33, 597
NcoI CCATGG 2 cut(s) 121, 603
NdeII GATC 2 cut(s) 429, 663
NlaIII CATG 6 cut(s) 125, 298, 416, 514, 607, 838
NlaIV GGNNCC 5 cut(s) 36, 330, 406, 601, 696
NmeAIII GCCGAG 1 cut(s) 723
NmuCI GTSAC 3 cut(s) 226, 478, 740
NspI RCATGY 1 cut(s) 416
PaeI GCATGC 1 cut(s) 416
PcsI WCGNNNNNNNCGW 2 cut(s) 141, 153
PfeI GAWTC 3 cut(s) 200, 236, 625
Pfl23II CGTACG 1 cut(s) 504
PkrI GCNGC 4 cut(s) 418, 488, 567, 724
PleI GAGTC 2 cut(s) 587, 754
PluTI GGCGCC 1 cut(s) 408
PpsI GAGTC 2 cut(s) 587, 754
PpuMI RGGWCCY 1 cut(s) 35
Psp5II RGGWCCY 1 cut(s) 35
Psp6I CCWGG 1 cut(s) 846
PspGI CCWGG 1 cut(s) 846
PspLI CGTACG 1 cut(s) 504
PspN4I GGNNCC 5 cut(s) 36, 330, 406, 601, 696
PspPI GGNCC 2 cut(s) 35, 328
PspPPI RGGWCCY 1 cut(s) 35
PstNI CAGNNNCTG 1 cut(s) 853
RsaI GTAC 5 cut(s) 212, 278, 299, 506, 731
RsaNI GTAC 5 cut(s) 211, 277, 298, 505, 730
SatI GCNGC 4 cut(s) 417, 487, 566, 723
Sau3AI GATC 2 cut(s) 429, 663
Sau96I GGNCC 2 cut(s) 35, 328
SchI GAGTC 2 cut(s) 588, 755
ScrFI CCNGG 3 cut(s) 33, 597, 848
SduI GDGCHC 1 cut(s) 101
SexAI ACCWGGT 1 cut(s) 846
SfoI GGCGCC 1 cut(s) 406
SinI GGWCC 2 cut(s) 35, 328
SphI GCATGC 1 cut(s) 416
Sse9I AATT 7 cut(s) 27, 63, 87, 323, 573, 583, 752
SsiI CCGC 4 cut(s) 486, 524, 677, 716
SspDI GGCGCC 1 cut(s) 404
SspMI CTAG 4 cut(s) 81, 161, 420, 816
StyD4I CCNGG 3 cut(s) 31, 595, 846
StyI CCWWGG 2 cut(s) 121, 603
TaaI ACNGT 2 cut(s) 232, 652
TaiI ACGT 3 cut(s) 150, 220, 264
TaqI TCGA 4 cut(s) 186, 234, 272, 515
TasI AATT 7 cut(s) 27, 63, 87, 323, 573, 583, 752
TatI WGTACW 2 cut(s) 297, 729
TauI GCSGC 1 cut(s) 489
TfiI GAWTC 3 cut(s) 200, 236, 625
TscAI CASTG 3 cut(s) 106, 235, 483
TseFI GTSAC 3 cut(s) 226, 478, 740
TseI GCWGC 3 cut(s) 416, 565, 722
Tsp45I GTSAC 3 cut(s) 226, 478, 740
TspDTI ATGAA 2 cut(s) 554, 823
TspGWI ACGGA 3 cut(s) 210, 294, 585
TspRI CASTG 3 cut(s) 106, 235, 483
VpaK11BI GGWCC 2 cut(s) 35, 328
XapI RAATTY 1 cut(s) 752
XceI RCATGY 1 cut(s) 416
XmiI GTMKAC 1 cut(s) 494
XspI CTAG 4 cut(s) 81, 161, 420, 816
ZraI GACGTC 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.