FvH4_6g20250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016886
Physical Location & Seq
Reverse (-)
13853903 .. 13856540
2638 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g20250.t1

Sequence Viewer

Length: 669 bp
ATGGCCGAGTTTCCAGACCATGCTCGACTGTGGCTTTCTTCTGATACTTTCTTCAACGACGTCGTCCCGTCTGAGTCTAACCTCCTCCAGAGTCGTTTTTCCTCCATGGACGACCTCGCTCATAGGCTTGCCGCTCTCACTTTGCTCAAACAACGTCGCGGTCTAGGCCTTCAGCGGTTCAGACCGCCGGTTCCGACCGGTTATCTTGATTCTCAGTACTGCCGGAGTTATCTCTGCGGCTTCTGCAACGGGAGTGGAGCTCACCTTTCCGGGACTGAGCTCTCTCACCACTACCCGTTCCCGAACCCGGTTCCTCCTCAGGTTGGTGATGGTAGCTTTCTGGAAGCTAGAGCTAGGGGTTTGAGTATGCATCATAACCAAGCCCAAATCGGAAACCAAAACCGCCTTTTCCAGTTTCAGGGAAATGGGATTGCCCCAATGGGTGTTTTTGAGAGGGTTTCCGGTGGTACCGGTGTTTTCATTCCCAGGGTTTCCGCTTCCACCATAACCACCATGAGTCCTGAGGTCAAGAAGAAACCTGGGTTGAAAAATAAGCGAGAGACTAGCGGCATTCAACAAAGGAACTCTATAAATAATTGCGCTGGTATGGGCGAAGAGCATCACCAGCTTCGTCCTGATGTTGTTTTACCTCAAGAGTGGACGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

223

Amino Acids

24.65

Weight (kDa)

9.0

Isoelectric Point (pI)

49.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016886)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g20250
malus_domestica MD12G1039600.v1.1
prunus_persica Prupe.7G095600_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0475841
rosa_laevigata RLG00000023809
rosa_multiflora Rmu_sc0000539.1_g000089 Rmu_sc0011491.1_g000001
rosa_roxburghii Rroxscaffold_6G00405800
rosa_rugosa Rorug03G0151700
rosa_samantha Rh3AG201500 Rh3BG231800 Rh3CG226500 Rh3DG227100
rosa_wichuraiana Rw3G018350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 63
Acc65I GGTACC 1 cut(s) 467
AccB1I GGYRCC 1 cut(s) 467
AccBSI CCGCTC 1 cut(s) 134
AccII CGCG 1 cut(s) 159
AciI CCGC 8 cut(s) 132, 159, 175, 185, 237, 403, 495, 567
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 155
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 3 cut(s) 218, 469, 665
AfiI CCNNNNNNNGG 3 cut(s) 307, 323, 418
AgeI ACCGGT 2 cut(s) 197, 470
AgsI TTSAA 3 cut(s) 55, 547, 575
AjnI CCWGG 2 cut(s) 485, 538
AluBI AGCT 6 cut(s) 260, 280, 336, 347, 353, 628
AluI AGCT 6 cut(s) 260, 280, 336, 347, 353, 628
Alw21I GWGCWC 2 cut(s) 262, 282
Alw26I GTCTC 1 cut(s) 554
AoxI GGCC 2 cut(s) 3, 166
AsiGI ACCGGT 2 cut(s) 197, 470
Asp718I GGTACC 1 cut(s) 467
AspLEI GCGC 1 cut(s) 602
AsuC2I CCSGG 2 cut(s) 271, 308
AsuHPI GGTGA 4 cut(s) 254, 278, 338, 614
AxyI CCTNAGG 2 cut(s) 318, 522
BaeI ACNNNNGTAYC 2 cut(s) 459, 492
BanI GGYRCC 1 cut(s) 467
BanII GRGCYC 2 cut(s) 262, 282
Bbv12I GWGCWC 2 cut(s) 262, 282
BccI CCATC 1 cut(s) 323
BciT130I CCWGG 2 cut(s) 487, 540
BcnI CCSGG 2 cut(s) 271, 308
BcoDI GTCTC 1 cut(s) 554
BfaI CTAG 4 cut(s) 164, 348, 354, 564
BisI GCNGC 3 cut(s) 132, 238, 568
BlsI GCNGC 3 cut(s) 133, 239, 569
BmcAI AGTACT 1 cut(s) 218
Bme1390I CCNGG 4 cut(s) 271, 308, 487, 540
BmiI GGNNCC 3 cut(s) 192, 312, 469
BmrFI CCNGG 4 cut(s) 271, 308, 487, 540
BmsI GCATC 2 cut(s) 379, 628
BplI GAGNNNNNCTC 2 cut(s) 244, 276
BpmI CTGGAG 1 cut(s) 71
BpuEI CTTGAG 1 cut(s) 636
BpuMI CCSGG 2 cut(s) 271, 308
BsaHI GRCGYC 1 cut(s) 60
BsaJI CCNNGG 4 cut(s) 105, 485, 486, 539
BsaWI WCCGGW 3 cut(s) 197, 461, 470
Bsc4I CCNNNNNNNGG 3 cut(s) 307, 323, 418
Bse118I RCCGGY 3 cut(s) 187, 197, 470
Bse1I ACTGG 1 cut(s) 412
Bse21I CCTNAGG 2 cut(s) 318, 522
BseBI CCWGG 2 cut(s) 487, 540
BseDI CCNNGG 4 cut(s) 105, 485, 486, 539
BseLI CCNNNNNNNGG 3 cut(s) 307, 323, 418
BseMII CTCAG 5 cut(s) 63, 227, 267, 332, 513
BseNI ACTGG 1 cut(s) 412
BseRI GAGGAG 2 cut(s) 74, 306
Bsh1236I CGCG 1 cut(s) 159
Bsh1285I CGRYCG 1 cut(s) 198
BshFI GGCC 2 cut(s) 5, 168
BshNI GGYRCC 1 cut(s) 467
BshTI ACCGGT 2 cut(s) 197, 470
BsiEI CGRYCG 1 cut(s) 198
BsiHKAI GWGCWC 2 cut(s) 262, 282
BsiSI CCGG 7 cut(s) 188, 198, 223, 270, 308, 462, 471
BslFI GGGAC 2 cut(s) 50, 286
BslI CCNNNNNNNGG 3 cut(s) 307, 323, 418
BsmAI GTCTC 1 cut(s) 554
BsmFI GGGAC 2 cut(s) 50, 286
BsmI GAATGC 1 cut(s) 570
BsnI GGCC 2 cut(s) 5, 168
Bsp1286I GDGCHC 2 cut(s) 262, 282
Bsp19I CCATGG 1 cut(s) 105
BspACI CCGC 8 cut(s) 132, 159, 175, 185, 237, 403, 495, 567
BspANI GGCC 2 cut(s) 5, 168
BspCNI CTCAG 5 cut(s) 64, 226, 268, 331, 514
BspFNI CGCG 1 cut(s) 159
BspLI GGNNCC 3 cut(s) 192, 312, 469
BspQI GCTCTTC 1 cut(s) 609
BspT107I GGYRCC 1 cut(s) 467
BsrBI CCGCTC 1 cut(s) 134
BsrFI RCCGGY 3 cut(s) 187, 197, 470
BsrI ACTGG 1 cut(s) 412
BssAI RCCGGY 3 cut(s) 187, 197, 470
BssECI CCNNGG 4 cut(s) 105, 485, 486, 539
BssNI GRCGYC 1 cut(s) 60
BssT1I CCWWGG 1 cut(s) 105
Bst2UI CCWGG 2 cut(s) 487, 540
Bst4CI ACNGT 1 cut(s) 30
Bst6I CTCTTC 1 cut(s) 609
BstACI GRCGYC 1 cut(s) 60
BstC8I GCNNGC 1 cut(s) 129
BstDEI CTNAG 5 cut(s) 72, 213, 276, 318, 522
BstDSI CCRYGG 1 cut(s) 105
BstFNI CGCG 1 cut(s) 159
BstHHI GCGC 1 cut(s) 602
BstMAI GTCTC 1 cut(s) 554
BstMCI CGRYCG 1 cut(s) 198
BstMWI GCNNNNNNNGC 3 cut(s) 165, 243, 625
BstNI CCWGG 2 cut(s) 487, 540
BstSCI CCNGG 4 cut(s) 269, 306, 485, 538
BstUI CGCG 1 cut(s) 159
Bsu36I CCTNAGG 2 cut(s) 318, 522
BsuRI GGCC 2 cut(s) 5, 168
BtgI CCRYGG 1 cut(s) 105
Cac8I GCNNGC 1 cut(s) 129
CfoI GCGC 1 cut(s) 602
Cfr10I RCCGGY 3 cut(s) 187, 197, 470
Csp6I GTAC 3 cut(s) 217, 468, 664
CspAI ACCGGT 2 cut(s) 197, 470
CspCI CAANNNNNGTGG 2 cut(s) 235, 270
CviAII CATG 3 cut(s) 20, 106, 514
CviQI GTAC 3 cut(s) 217, 468, 664
DdeI CTNAG 5 cut(s) 72, 213, 276, 318, 522
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 609
EarI CTCTTC 1 cut(s) 609
Ecl136II GAGCTC 2 cut(s) 260, 280
Eco130I CCWWGG 1 cut(s) 105
Eco147I AGGCCT 1 cut(s) 168
Eco24I GRGCYC 2 cut(s) 262, 282
Eco53kI GAGCTC 2 cut(s) 260, 280
Eco57I CTGAAG 1 cut(s) 155
Eco81I CCTNAGG 2 cut(s) 318, 522
EcoICRI GAGCTC 2 cut(s) 260, 280
EcoRII CCWGG 2 cut(s) 485, 538
EcoT14I CCWWGG 1 cut(s) 105
EcoT22I ATGCAT 1 cut(s) 372
EcoT38I GRGCYC 2 cut(s) 262, 282
ErhI CCWWGG 1 cut(s) 105
FaeI CATG 3 cut(s) 23, 109, 517
FaiI YATR 9 cut(s) 21, 107, 123, 368, 375, 506, 515, 590, 608
FaqI GGGAC 2 cut(s) 50, 286
FatI CATG 3 cut(s) 19, 105, 513
Fnu4HI GCNGC 3 cut(s) 132, 238, 568
FriOI GRGCYC 2 cut(s) 262, 282
Fsp4HI GCNGC 3 cut(s) 132, 238, 568
FspBI CTAG 4 cut(s) 164, 348, 354, 564
GlaI GCGC 1 cut(s) 601
GluI GCNGC 3 cut(s) 132, 238, 568
GsuI CTGGAG 1 cut(s) 71
HaeIII GGCC 2 cut(s) 5, 168
HapII CCGG 7 cut(s) 188, 198, 223, 270, 308, 462, 471
HhaI GCGC 1 cut(s) 602
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 3 cut(s) 23, 109, 517
Hin6I GCGC 1 cut(s) 600
HinP1I GCGC 1 cut(s) 600
HinfI GANTC 4 cut(s) 74, 91, 209, 517
HpaII CCGG 7 cut(s) 188, 198, 223, 270, 308, 462, 471
HphI GGTGA 4 cut(s) 254, 278, 338, 614
Hpy166II GTNNAC 1 cut(s) 660
Hpy188I TCNGA 5 cut(s) 43, 73, 182, 195, 392
Hpy188III TCNNGA 9 cut(s) 14, 88, 206, 301, 341, 521, 529, 635, 653
Hpy8I GTNNAC 1 cut(s) 660
Hpy99I CGWCG 3 cut(s) 62, 65, 159
HpyAV CCTTC 1 cut(s) 179
HpyCH4III ACNGT 1 cut(s) 30
HpyCH4IV ACGT 3 cut(s) 60, 154, 662
HpyCH4V TGCA 2 cut(s) 246, 370
HpyF10VI GCNNNNNNNGC 3 cut(s) 165, 243, 625
HpyF3I CTNAG 5 cut(s) 72, 213, 276, 318, 522
HpySE526I ACGT 3 cut(s) 60, 154, 662
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 3 cut(s) 23, 109, 517
HspAI GCGC 1 cut(s) 600
KpnI GGTACC 1 cut(s) 471
LguI GCTCTTC 1 cut(s) 609
LmnI GCTCC 1 cut(s) 257
LweI GCATC 2 cut(s) 379, 628
MaeI CTAG 4 cut(s) 164, 348, 354, 564
MaeII ACGT 3 cut(s) 60, 154, 662
MbiI CCGCTC 1 cut(s) 134
MboII GAAGA 4 cut(s) 30, 43, 544, 626
MhlI GDGCHC 2 cut(s) 262, 282
MluCI AATT 1 cut(s) 595
MlyI GAGTC 3 cut(s) 83, 100, 526
MmeI TCCRAC 1 cut(s) 218
MnlI CCTC 9 cut(s) 92, 95, 112, 125, 324, 327, 447, 517, 660
Mph1103I ATGCAT 1 cut(s) 372
MspA1I CMGCKG 1 cut(s) 175
MspI CCGG 7 cut(s) 188, 198, 223, 270, 308, 462, 471
MspR9I CCNGG 4 cut(s) 271, 308, 487, 540
Mva1269I GAATGC 1 cut(s) 570
MvaI CCWGG 2 cut(s) 487, 540
MvnI CGCG 1 cut(s) 159
MwoI GCNNNNNNNGC 3 cut(s) 165, 243, 625
NciI CCSGG 2 cut(s) 271, 308
NcoI CCATGG 1 cut(s) 105
NlaIII CATG 3 cut(s) 23, 109, 517
NlaIV GGNNCC 3 cut(s) 192, 312, 469
NmeAIII GCCGAG 1 cut(s) 31
NsiI ATGCAT 1 cut(s) 372
PasI CCCWGGG 1 cut(s) 486
PceI AGGCCT 1 cut(s) 168
PciSI GCTCTTC 1 cut(s) 609
PctI GAATGC 1 cut(s) 570
PfeI GAWTC 1 cut(s) 209
PflFI GACNNNGTC 1 cut(s) 62
PfoI TCCNGGA 1 cut(s) 269
PinAI ACCGGT 2 cut(s) 197, 470
PkrI GCNGC 3 cut(s) 133, 239, 569
PleI GAGTC 3 cut(s) 82, 99, 525
PpsI GAGTC 3 cut(s) 82, 99, 525
Psp124BI GAGCTC 2 cut(s) 262, 282
Psp6I CCWGG 2 cut(s) 485, 538
PspGI CCWGG 2 cut(s) 485, 538
PspN4I GGNNCC 3 cut(s) 192, 312, 469
PsyI GACNNNGTC 1 cut(s) 62
RsaI GTAC 3 cut(s) 218, 469, 665
RsaNI GTAC 3 cut(s) 217, 468, 664
SacI GAGCTC 2 cut(s) 262, 282
SapI GCTCTTC 1 cut(s) 609
SatI GCNGC 3 cut(s) 132, 238, 568
ScaI AGTACT 1 cut(s) 218
SchI GAGTC 3 cut(s) 83, 100, 526
ScrFI CCNGG 4 cut(s) 271, 308, 487, 540
SduI GDGCHC 2 cut(s) 262, 282
SfaNI GCATC 2 cut(s) 379, 628
SmlI CTYRAG 1 cut(s) 651
SmoI CTYRAG 1 cut(s) 651
Sse9I AATT 1 cut(s) 595
SseBI AGGCCT 1 cut(s) 168
SsiI CCGC 8 cut(s) 132, 159, 175, 185, 237, 403, 495, 567
SspMI CTAG 4 cut(s) 164, 348, 354, 564
SstI GAGCTC 2 cut(s) 262, 282
StuI AGGCCT 1 cut(s) 168
StyD4I CCNGG 4 cut(s) 269, 306, 485, 538
StyI CCWWGG 1 cut(s) 105
TaaI ACNGT 1 cut(s) 30
TaiI ACGT 3 cut(s) 63, 157, 665
TaqI TCGA 1 cut(s) 25
TasI AATT 1 cut(s) 595
TatI WGTACW 1 cut(s) 216
TauI GCSGC 3 cut(s) 134, 240, 570
TfiI GAWTC 1 cut(s) 209
TspDTI ATGAA 1 cut(s) 469
Tth111I GACNNNGTC 1 cut(s) 62
XspI CTAG 4 cut(s) 164, 348, 354, 564
ZraI GACGTC 1 cut(s) 61
ZrmI AGTACT 1 cut(s) 218
Zsp2I ATGCAT 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.