MD12G1039600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016886
Physical Location & Seq
Reverse (-)
4301505 .. 4303644
2140 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1039600.v1.1.491

Sequence Viewer

Length: 597 bp
ATGCTAAAAATGGCTGGGTTTCCACCAAATCCCGACGACGGTGAACTGTGGCTTCCATCTGATATTTTTTTCAACGAAGCAGCTTCTTCAACCTCCACACTTAGCCGTCACCATTTCTCTAATACCACGGACAACCTCGCTCGGCAGTTGGCTTCTCTCTCTCTCCTCAAACAATACCAAAGTCCAAGCCTTTCAAATCTTCAGAGTTTGCACCGGGTCAGACCGGCGGTCCGGTCCACCCAGCTTGATTATTTGACCCAGAGGTACTTTAATCTCGGCGACAGCCATGGTTTTGTACAAAAAGGTCCTATTTTTCATGGGTACGGGACTCAACACCTCCATTACCACTATCAGTTTATGAACCCGGCTCAACTTCAGGTTGGTAGCTTTTTGGAAACCAGAAATGGGATTCAGAAGAGACAGCAAAACCGTGTTTTGCCATTCCAGGGTAGAGGGAATGGATCAATGGGTCGTTTTGGGAGGGTTTGTGGAGGCACAGGCGTGTTTCATCCCAGGGTTTTGCATTCCACAACCACCGCCACCGCCACCGCCAGTAATCCGGAGGTCAAAGGGAAGCAGGGTCAGTTTAAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

199

Amino Acids

22.13

Weight (kDa)

10.23

Isoelectric Point (pI)

38.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016886)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g20250
malus_domestica MD12G1039600.v1.1
prunus_persica Prupe.7G095600_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0475841
rosa_laevigata RLG00000023809
rosa_multiflora Rmu_sc0000539.1_g000089 Rmu_sc0011491.1_g000001
rosa_roxburghii Rroxscaffold_6G00405800
rosa_rugosa Rorug03G0151700
rosa_samantha Rh3AG201500 Rh3BG231800 Rh3CG226500 Rh3DG227100
rosa_wichuraiana Rw3G018350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 559
AciI CCGC 4 cut(s) 227, 537, 543, 549
AclWI GGATC 1 cut(s) 469
AcuI CTGAAG 2 cut(s) 185, 359
AfaI GTAC 3 cut(s) 266, 297, 323
AfiI CCNNNNNNNGG 3 cut(s) 38, 405, 446
AgsI TTSAA 3 cut(s) 73, 90, 195
AhdI GACNNNNNGTC 1 cut(s) 227
AjnI CCWGG 2 cut(s) 444, 512
AleI CACNNNNGTG 1 cut(s) 500
AluBI AGCT 3 cut(s) 83, 244, 387
AluI AGCT 3 cut(s) 83, 244, 387
Alw26I GTCTC 1 cut(s) 412
AlwI GGATC 1 cut(s) 469
Aor13HI TCCGGA 1 cut(s) 559
ApeKI GCWGC 1 cut(s) 80
AspS9I GGNCC 3 cut(s) 229, 234, 305
AsuC2I CCSGG 2 cut(s) 215, 365
AsuHPI GGTGA 2 cut(s) 53, 101
AvaII GGWCC 3 cut(s) 229, 234, 305
BbvI GCAGC 1 cut(s) 92
BccI CCATC 1 cut(s) 64
BceAI ACGGC 1 cut(s) 90
BciT130I CCWGG 2 cut(s) 446, 514
BcnI CCSGG 2 cut(s) 215, 365
BcoDI GTCTC 1 cut(s) 412
BisI GCNGC 1 cut(s) 81
BlsI GCNGC 1 cut(s) 82
Bme1390I CCNGG 4 cut(s) 215, 365, 446, 514
Bme18I GGWCC 3 cut(s) 229, 234, 305
BmeRI GACNNNNNGTC 1 cut(s) 227
BmgT120I GGNCC 3 cut(s) 229, 234, 305
BmrFI CCNGG 4 cut(s) 215, 365, 446, 514
BpuMI CCSGG 2 cut(s) 215, 365
BsaJI CCNNGG 5 cut(s) 126, 286, 445, 512, 513
BsaWI WCCGGW 2 cut(s) 231, 559
BsaXI ACNNNNNCTCC 2 cut(s) 472, 502
Bsc4I CCNNNNNNNGG 3 cut(s) 38, 405, 446
Bse118I RCCGGY 1 cut(s) 223
Bse1I ACTGG 1 cut(s) 552
BseAI TCCGGA 1 cut(s) 559
BseBI CCWGG 2 cut(s) 446, 514
BseDI CCNNGG 5 cut(s) 126, 286, 445, 512, 513
BseGI GGATG 1 cut(s) 508
BseLI CCNNNNNNNGG 3 cut(s) 38, 405, 446
BseNI ACTGG 1 cut(s) 552
BseRI GAGGAG 1 cut(s) 155
BseXI GCAGC 1 cut(s) 92
BseYI CCCAGC 2 cut(s) 14, 240
BsiSI CCGG 5 cut(s) 214, 224, 232, 365, 560
BslFI GGGAC 1 cut(s) 340
BslI CCNNNNNNNGG 3 cut(s) 38, 405, 446
BsmAI GTCTC 1 cut(s) 412
BsmFI GGGAC 1 cut(s) 340
BsmI GAATGC 1 cut(s) 523
Bsp13I TCCGGA 1 cut(s) 559
Bsp1407I TGTACA 1 cut(s) 295
Bsp143I GATC 1 cut(s) 461
Bsp19I CCATGG 1 cut(s) 286
BspACI CCGC 4 cut(s) 227, 537, 543, 549
BspEI TCCGGA 1 cut(s) 559
BspPI GGATC 1 cut(s) 469
BsrFI RCCGGY 1 cut(s) 223
BsrGI TGTACA 1 cut(s) 295
BsrI ACTGG 1 cut(s) 552
BssAI RCCGGY 1 cut(s) 223
BssECI CCNNGG 5 cut(s) 126, 286, 445, 512, 513
BssMI GATC 1 cut(s) 461
BssT1I CCWWGG 1 cut(s) 286
Bst2UI CCWGG 2 cut(s) 446, 514
Bst4CI ACNGT 3 cut(s) 41, 48, 431
Bst6I CTCTTC 1 cut(s) 410
BstAUI TGTACA 1 cut(s) 295
BstDEI CTNAG 1 cut(s) 101
BstDSI CCRYGG 2 cut(s) 126, 286
BstF5I GGATG 1 cut(s) 508
BstKTI GATC 1 cut(s) 464
BstMAI GTCTC 1 cut(s) 412
BstMBI GATC 1 cut(s) 461
BstNI CCWGG 2 cut(s) 446, 514
BstSCI CCNGG 4 cut(s) 213, 363, 444, 512
BstV1I GCAGC 1 cut(s) 92
BtgI CCRYGG 2 cut(s) 126, 286
BtsCI GGATG 1 cut(s) 508
Cfr10I RCCGGY 1 cut(s) 223
Cfr13I GGNCC 3 cut(s) 229, 234, 305
CpoI CGGWCCG 1 cut(s) 229
Csp6I GTAC 3 cut(s) 265, 296, 322
CspCI CAANNNNNGTGG 2 cut(s) 226, 261
CspI CGGWCCG 1 cut(s) 229
CviAII CATG 2 cut(s) 287, 317
CviQI GTAC 3 cut(s) 265, 296, 322
DdeI CTNAG 1 cut(s) 101
DpnI GATC 1 cut(s) 463
DpnII GATC 1 cut(s) 461
DriI GACNNNNNGTC 1 cut(s) 227
Eam1104I CTCTTC 1 cut(s) 410
Eam1105I GACNNNNNGTC 1 cut(s) 227
EarI CTCTTC 1 cut(s) 410
Eco130I CCWWGG 1 cut(s) 286
Eco47I GGWCC 3 cut(s) 229, 234, 305
Eco57I CTGAAG 2 cut(s) 185, 359
EcoO109I RGGNCCY 1 cut(s) 305
EcoRII CCWGG 2 cut(s) 444, 512
EcoT14I CCWWGG 1 cut(s) 286
ErhI CCWWGG 1 cut(s) 286
FaeI CATG 2 cut(s) 290, 320
FaiI YATR 3 cut(s) 288, 318, 359
FaqI GGGAC 1 cut(s) 340
FatI CATG 2 cut(s) 286, 316
Fnu4HI GCNGC 1 cut(s) 81
FokI GGATG 1 cut(s) 495
Fsp4HI GCNGC 1 cut(s) 81
GluI GCNGC 1 cut(s) 81
GsaI CCCAGC 2 cut(s) 18, 244
HapII CCGG 5 cut(s) 214, 224, 232, 365, 560
Hin1II CATG 2 cut(s) 290, 320
HinfI GANTC 2 cut(s) 328, 409
HpaII CCGG 5 cut(s) 214, 224, 232, 365, 560
HphI GGTGA 2 cut(s) 53, 101
Hpy166II GTNNAC 2 cut(s) 44, 237
Hpy188I TCNGA 4 cut(s) 61, 204, 221, 414
Hpy188III TCNNGA 2 cut(s) 32, 560
Hpy8I GTNNAC 2 cut(s) 44, 237
Hpy99I CGWCG 2 cut(s) 38, 41
HpyCH4III ACNGT 3 cut(s) 41, 48, 431
HpyCH4V TGCA 2 cut(s) 211, 523
HpyF3I CTNAG 1 cut(s) 101
Hsp92II CATG 2 cut(s) 290, 320
Kpn2I TCCGGA 1 cut(s) 559
Kzo9I GATC 1 cut(s) 461
Lsp1109I GCAGC 1 cut(s) 92
MaeIII GTNAC 1 cut(s) 107
MalI GATC 1 cut(s) 463
MboI GATC 1 cut(s) 461
MboII GAAGA 3 cut(s) 78, 191, 427
MlyI GAGTC 1 cut(s) 322
MnlI CCTC 9 cut(s) 103, 146, 176, 255, 347, 446, 474, 485, 556
MroI TCCGGA 1 cut(s) 559
MseI TTAA 2 cut(s) 270, 588
MslI CAYNNNNRTG 1 cut(s) 500
MspI CCGG 5 cut(s) 214, 224, 232, 365, 560
MspR9I CCNGG 4 cut(s) 215, 365, 446, 514
Mva1269I GAATGC 1 cut(s) 523
MvaI CCWGG 2 cut(s) 446, 514
NciI CCSGG 2 cut(s) 215, 365
NcoI CCATGG 1 cut(s) 286
NdeII GATC 1 cut(s) 461
NlaIII CATG 2 cut(s) 290, 320
NmeAIII GCCGAG 2 cut(s) 121, 255
NmuCI GTSAC 1 cut(s) 107
OliI CACNNNNGTG 1 cut(s) 500
PasI CCCWGGG 1 cut(s) 513
PctI GAATGC 1 cut(s) 523
PfeI GAWTC 1 cut(s) 409
PkrI GCNGC 1 cut(s) 82
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
PpuMI RGGWCCY 1 cut(s) 305
Psp5II RGGWCCY 1 cut(s) 305
Psp6I CCWGG 2 cut(s) 444, 512
PspFI CCCAGC 2 cut(s) 14, 240
PspGI CCWGG 2 cut(s) 444, 512
PspPI GGNCC 3 cut(s) 229, 234, 305
PspPPI RGGWCCY 1 cut(s) 305
RsaI GTAC 3 cut(s) 266, 297, 323
RsaNI GTAC 3 cut(s) 265, 296, 322
RseI CAYNNNNRTG 1 cut(s) 500
Rsr2I CGGWCCG 1 cut(s) 229
RsrII CGGWCCG 1 cut(s) 229
SaqAI TTAA 2 cut(s) 270, 588
SatI GCNGC 1 cut(s) 81
Sau3AI GATC 1 cut(s) 461
Sau96I GGNCC 3 cut(s) 229, 234, 305
SchI GAGTC 1 cut(s) 322
ScrFI CCNGG 4 cut(s) 215, 365, 446, 514
SinI GGWCC 3 cut(s) 229, 234, 305
SmiMI CAYNNNNRTG 1 cut(s) 500
SsiI CCGC 4 cut(s) 227, 537, 543, 549
StyD4I CCNGG 4 cut(s) 213, 363, 444, 512
StyI CCWWGG 1 cut(s) 286
TaaI ACNGT 3 cut(s) 41, 48, 431
TatI WGTACW 1 cut(s) 295
TfiI GAWTC 1 cut(s) 409
Tru1I TTAA 2 cut(s) 270, 588
Tru9I TTAA 2 cut(s) 270, 588
TseFI GTSAC 1 cut(s) 107
TseI GCWGC 1 cut(s) 80
Tsp45I GTSAC 1 cut(s) 107
TspDTI ATGAA 3 cut(s) 305, 374, 497
TspGWI ACGGA 1 cut(s) 143
VpaK11BI GGWCC 3 cut(s) 229, 234, 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.