Rmu_sc0000539.1_g000089

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000539.1
OG0016886
Physical Location & Seq
Reverse (-)
389344 .. 395644
6301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000539.1_g000089.1.cds

Sequence Viewer

Length: 1467 bp
atggccgagtttcctccgaatgttgacgacgctcgactctggcttccctctgatactttcttcaacgacgtcgtcccgtctgaatttagcccccaccagagtccgttttcctccatggacgacctcgctcatcaactcgccgctctcactttgctcaaacagcgtcgcagtctacgccttcagcggttcagaccgccggttccgaccggtcagcttgattttcagttccagcgttacttcaacggcttctccgggactgagctctgtcaccactacccgttcccgaacccggttcagcttcagattggtgatggtggctttctggaacctagagctcgggctttgaggaggcatcataaccaagcccaaatccgaaaccaaaaccgccttttccaatttcagggaaatgggattggcccaatgggtggtgcttttgagagggtttccggaggcaccggtgtttttcacccccgggttttggattccaccaaaaccaccaggagtgttgaggtcaaaaagaaacctgggttgagaagtaggcaagagattagaggcattcaacaaaggaactctatgaataaatgcgttggtatgggcgaagagcatcaccagctttgtcctgataacggttccaaaatcgaaaccatcccaaaatccaaatgctccttaaatcgatctagggctccgacgaaggtaggggacgaagcagctggcggattgaggcggcaaagcaaccgacgaagggaggctgcgaatcaggcgaagcaaggctgcaaagcaaccggcgaagtgaggcggcaaagcaaccagcgaagtgaggtggcgaagggaggctgcgaagcctgcgagacgaggctgcaaaaccagcgaagggagcatcaaatctctgatactctttcgctttcctttcactatatttctaagcttggcttataccagctcatggatccctttgatttggtgcaaaaaggtattgctccgcttcggatctttcacttggttttgggattgatttcggatgaagctaatgaaagcaatgctgatgtgagcaaacgcaaaaaggccaggatagtatactactgcggatcgagaaacctcgacccgaataagcttttagacccagacccgactcaaggtgatctccgctgttcggacatcccagaccaccgcactggtcggaaacgactagtgctcctagggagcagaaaccgaactacccctgatctgacacccagtccgattgagtcttctcataacattcaagccgggcttcttctcatgcctcctcctcaacttagaacactatctactcctgctagtgcctcaattgaagctccaagcagtgaagctgaagatattgtgaagaggaagaaccagtcagtagcttgggatcactttgtgaagatgaagaatccggatggcacaaagatggtgaaaccgagagcaaagtgcaagtactgcccctag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

488

Amino Acids

54.79

Weight (kDa)

9.83

Isoelectric Point (pI)

60.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016886)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g20250
malus_domestica MD12G1039600.v1.1
prunus_persica Prupe.7G095600_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0475841
rosa_laevigata RLG00000023809
rosa_multiflora Rmu_sc0000539.1_g000089 Rmu_sc0011491.1_g000001
rosa_roxburghii Rroxscaffold_6G00405800
rosa_rugosa Rorug03G0151700
rosa_samantha Rh3AG201500 Rh3BG231800 Rh3CG226500 Rh3DG227100
rosa_wichuraiana Rw3G018350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 72
AccB1I GGYRCC 1 cut(s) 452
AccB7I CCANNNNNTGG 2 cut(s) 425, 943
AccBSI CCGCTC 1 cut(s) 143
AccI GTMKAC 2 cut(s) 172, 1074
AccIII TCCGGA 2 cut(s) 446, 1414
AclWI GGATC 5 cut(s) 941, 954, 995, 1093, 1398
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 83
AcuI CTGAAG 3 cut(s) 164, 284, 1371
AcyI GRCGYC 1 cut(s) 69
AdeI CACNNNGTG 1 cut(s) 1399
AfaI GTAC 1 cut(s) 1457
AgeI ACCGGT 2 cut(s) 206, 455
AgsI TTSAA 5 cut(s) 64, 241, 560, 1262, 1331
AhdI GACNNNNNGTC 1 cut(s) 1233
AhlI ACTAGT 1 cut(s) 1186
AjnI CCWGG 3 cut(s) 497, 523, 1064
Alw21I GWGCWC 3 cut(s) 264, 337, 1194
Alw26I GTCTC 1 cut(s) 842
AlwI GGATC 5 cut(s) 941, 954, 995, 1093, 1398
Ama87I CYCGRG 2 cut(s) 336, 471
Aor13HI TCCGGA 2 cut(s) 446, 1414
AoxI GGCC 3 cut(s) 3, 415, 1062
ApeKI GCWGC 5 cut(s) 707, 749, 771, 834, 856
ApoI RAATTY 1 cut(s) 83
AsiGI ACCGGT 2 cut(s) 206, 455
AspA2I CCTAGG 1 cut(s) 1195
AspS9I GGNCC 1 cut(s) 416
AsuC2I CCSGG 5 cut(s) 253, 290, 472, 473, 1267
AsuHPI GGTGA 6 cut(s) 260, 320, 458, 599, 1148, 1444
AvaI CYCGRG 2 cut(s) 336, 471
AvrII CCTAGG 1 cut(s) 1195
BamHI GGATCC 1 cut(s) 946
BanI GGYRCC 1 cut(s) 452
BanII GRGCYC 3 cut(s) 264, 337, 685
BbsI GAAGAC 1 cut(s) 1239
Bbv12I GWGCWC 3 cut(s) 264, 337, 1194
BbvI GCAGC 5 cut(s) 719, 736, 758, 821, 843
BccI CCATC 4 cut(s) 305, 653, 1412, 1423
BceAI ACGGC 1 cut(s) 259
BciT130I CCWGG 3 cut(s) 499, 525, 1066
BcnI CCSGG 5 cut(s) 253, 290, 472, 473, 1267
BcoDI GTCTC 1 cut(s) 842
BcuI ACTAGT 1 cut(s) 1186
BfaI CTAG 6 cut(s) 330, 678, 1187, 1196, 1317, 1465
BisI GCNGC 8 cut(s) 141, 708, 725, 750, 772, 797, 835, 857
BlnI CCTAGG 1 cut(s) 1195
BlsI GCNGC 8 cut(s) 142, 709, 726, 751, 773, 798, 836, 858
BmcAI AGTACT 1 cut(s) 1457
Bme1390I CCNGG 8 cut(s) 253, 290, 472, 473, 499, 525, 1066, 1267
BmeRI GACNNNNNGTC 1 cut(s) 1233
BmeT110I CYCGRG 2 cut(s) 336, 471
BmgT120I GGNCC 1 cut(s) 416
BmiI GGNNCC 6 cut(s) 201, 327, 454, 631, 684, 948
BmrFI CCNGG 8 cut(s) 253, 290, 472, 473, 499, 525, 1066, 1267
BmrI ACTGGG 1 cut(s) 1227
BmsI GCATC 3 cut(s) 361, 613, 886
BmuI ACTGGG 1 cut(s) 1227
BpiI GAAGAC 1 cut(s) 1239
BpuEI CTTGAG 1 cut(s) 1116
BpuMI CCSGG 5 cut(s) 253, 290, 472, 473, 1267
Bsa29I ATCGAT 1 cut(s) 673
BsaHI GRCGYC 1 cut(s) 69
BsaJI CCNNGG 5 cut(s) 114, 470, 471, 524, 1195
BsaWI WCCGGW 4 cut(s) 206, 446, 455, 1414
BsaXI ACNNNNNCTCC 4 cut(s) 233, 263, 1176, 1206
Bse118I RCCGGY 4 cut(s) 196, 206, 455, 782
Bse1I ACTGG 3 cut(s) 1177, 1233, 1375
Bse3DI GCAATG 1 cut(s) 1042
BseAI TCCGGA 2 cut(s) 446, 1414
BseBI CCWGG 3 cut(s) 499, 525, 1066
BseCI ATCGAT 1 cut(s) 673
BseDI CCNNGG 5 cut(s) 114, 470, 471, 524, 1195
BseGI GGATG 4 cut(s) 645, 1024, 1155, 1423
BseMI GCAATG 1 cut(s) 1042
BseMII CTCAG 1 cut(s) 249
BseNI ACTGG 3 cut(s) 1177, 1233, 1375
BseRI GAGGAG 3 cut(s) 361, 1275, 1278
BseXI GCAGC 5 cut(s) 719, 736, 758, 821, 843
Bsh1285I CGRYCG 1 cut(s) 207
BshFI GGCC 3 cut(s) 5, 417, 1064
BshNI GGYRCC 1 cut(s) 452
BshTI ACCGGT 2 cut(s) 206, 455
BshVI ATCGAT 1 cut(s) 673
BsiEI CGRYCG 1 cut(s) 207
BsiHKAI GWGCWC 3 cut(s) 264, 337, 1194
BsiHKCI CYCGRG 2 cut(s) 336, 471
BslFI GGGAC 3 cut(s) 59, 268, 713
BsmAI GTCTC 1 cut(s) 842
BsmBI CGTCTC 1 cut(s) 842
BsmFI GGGAC 3 cut(s) 59, 268, 713
BsmI GAATGC 1 cut(s) 555
BsnI GGCC 3 cut(s) 5, 417, 1064
BsoBI CYCGRG 2 cut(s) 336, 471
Bsp1286I GDGCHC 4 cut(s) 264, 337, 685, 1194
Bsp13I TCCGGA 2 cut(s) 446, 1414
Bsp143I GATC 7 cut(s) 674, 946, 987, 1085, 1138, 1222, 1390
Bsp19I CCATGG 1 cut(s) 114
BspANI GGCC 3 cut(s) 5, 417, 1064
BspCNI CTCAG 1 cut(s) 250
BspDI ATCGAT 1 cut(s) 673
BspEI TCCGGA 2 cut(s) 446, 1414
BspLI GGNNCC 6 cut(s) 201, 327, 454, 631, 684, 948
BspPI GGATC 5 cut(s) 941, 954, 995, 1093, 1398
BspQI GCTCTTC 1 cut(s) 594
BspT107I GGYRCC 1 cut(s) 452
BsrBI CCGCTC 1 cut(s) 143
BsrDI GCAATG 1 cut(s) 1042
BsrFI RCCGGY 4 cut(s) 196, 206, 455, 782
BsrI ACTGG 3 cut(s) 1177, 1233, 1375
BssAI RCCGGY 4 cut(s) 196, 206, 455, 782
BssECI CCNNGG 5 cut(s) 114, 470, 471, 524, 1195
BssMI GATC 7 cut(s) 674, 946, 987, 1085, 1138, 1222, 1390
BssNAI GTATAC 1 cut(s) 1075
BssNI GRCGYC 1 cut(s) 69
BssT1I CCWWGG 2 cut(s) 114, 1195
Bst1107I GTATAC 1 cut(s) 1075
Bst2UI CCWGG 3 cut(s) 499, 525, 1066
Bst4CI ACNGT 1 cut(s) 629
Bst6I CTCTTC 2 cut(s) 594, 1358
BstACI GRCGYC 1 cut(s) 69
BstAPI GCANNNNNTGC 1 cut(s) 1458
BstC8I GCNNGC 2 cut(s) 712, 844
BstDEI CTNAG 3 cut(s) 258, 921, 1295
BstDSI CCRYGG 1 cut(s) 114
BstF5I GGATG 4 cut(s) 645, 1024, 1155, 1423
BstKTI GATC 7 cut(s) 677, 949, 990, 1088, 1141, 1225, 1393
BstMAI GTCTC 1 cut(s) 842
BstMBI GATC 7 cut(s) 674, 946, 987, 1085, 1138, 1222, 1390
BstMCI CGRYCG 1 cut(s) 207
BstMWI GCNNNNNNNGC 8 cut(s) 160, 174, 610, 758, 843, 865, 874, 1458
BstNI CCWGG 3 cut(s) 499, 525, 1066
BstSCI CCNGG 8 cut(s) 251, 288, 470, 471, 497, 523, 1064, 1265
BstV1I GCAGC 5 cut(s) 719, 736, 758, 821, 843
BstV2I GAAGAC 1 cut(s) 1239
BstX2I RGATCY 2 cut(s) 946, 987
BstXI CCANNNNNNTGG 1 cut(s) 1172
BstYI RGATCY 2 cut(s) 946, 987
BstZ17I GTATAC 1 cut(s) 1075
Bsu15I ATCGAT 1 cut(s) 673
BsuRI GGCC 3 cut(s) 5, 417, 1064
BsuTUI ATCGAT 1 cut(s) 673
BtgI CCRYGG 1 cut(s) 114
BtsCI GGATG 4 cut(s) 645, 1024, 1155, 1423
BtsI GCAGTG 1 cut(s) 1348
BtsIMutI CAGTG 2 cut(s) 1170, 1348
Cac8I GCNNGC 2 cut(s) 712, 844
Cfr10I RCCGGY 4 cut(s) 196, 206, 455, 782
Cfr13I GGNCC 1 cut(s) 416
Cfr9I CCCGGG 1 cut(s) 471
ClaI ATCGAT 1 cut(s) 673
CseI GACGC 2 cut(s) 38, 152
Csp6I GTAC 1 cut(s) 1456
CspAI ACCGGT 2 cut(s) 206, 455
CviAII CATG 3 cut(s) 115, 943, 1279
CviQI GTAC 1 cut(s) 1456
DdeI CTNAG 3 cut(s) 258, 921, 1295
DpnI GATC 7 cut(s) 676, 948, 989, 1087, 1140, 1224, 1392
DpnII GATC 7 cut(s) 674, 946, 987, 1085, 1138, 1222, 1390
DraIII CACNNNGTG 1 cut(s) 1399
DriI GACNNNNNGTC 1 cut(s) 1233
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 594, 1358
Eam1105I GACNNNNNGTC 1 cut(s) 1233
EarI CTCTTC 2 cut(s) 594, 1358
EciI GGCGGA 1 cut(s) 729
Ecl136II GAGCTC 2 cut(s) 262, 335
Eco130I CCWWGG 2 cut(s) 114, 1195
Eco24I GRGCYC 3 cut(s) 264, 337, 685
Eco53kI GAGCTC 2 cut(s) 262, 335
Eco57I CTGAAG 3 cut(s) 164, 284, 1371
Eco88I CYCGRG 2 cut(s) 336, 471
EcoICRI GAGCTC 2 cut(s) 262, 335
EcoRII CCWGG 3 cut(s) 497, 523, 1064
EcoT14I CCWWGG 2 cut(s) 114, 1195
EcoT38I GRGCYC 3 cut(s) 264, 337, 685
ErhI CCWWGG 2 cut(s) 114, 1195
Esp3I CGTCTC 1 cut(s) 842
FaeI CATG 3 cut(s) 118, 946, 1282
FalI AAGNNNNNCTT 4 cut(s) 914, 946, 1254, 1286
FaqI GGGAC 3 cut(s) 59, 268, 713
FatI CATG 3 cut(s) 114, 942, 1278
FblI GTMKAC 2 cut(s) 172, 1074
Fnu4HI GCNGC 8 cut(s) 141, 708, 725, 750, 772, 797, 835, 857
FokI GGATG 4 cut(s) 632, 1031, 1142, 1430
FriOI GRGCYC 3 cut(s) 264, 337, 685
Fsp4HI GCNGC 8 cut(s) 141, 708, 725, 750, 772, 797, 835, 857
FspBI CTAG 6 cut(s) 330, 678, 1187, 1196, 1317, 1465
GluI GCNGC 8 cut(s) 141, 708, 725, 750, 772, 797, 835, 857
HaeIII GGCC 3 cut(s) 5, 417, 1064
HgaI GACGC 2 cut(s) 38, 152
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 3 cut(s) 118, 946, 1282
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HindIII AAGCTT 2 cut(s) 923, 1109
HinfI GANTC 7 cut(s) 36, 100, 482, 754, 1129, 1244, 1411
HphI GGTGA 6 cut(s) 260, 320, 458, 599, 1148, 1444
Hpy166II GTNNAC 3 cut(s) 25, 173, 1075
Hpy188III TCNNGA 6 cut(s) 283, 323, 447, 620, 1089, 1415
Hpy8I GTNNAC 3 cut(s) 25, 173, 1075
Hpy99I CGWCG 6 cut(s) 32, 71, 74, 168, 691, 741
HpyAV CCTTC 5 cut(s) 188, 685, 735, 820, 864
HpyCH4III ACNGT 1 cut(s) 629
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 4 cut(s) 774, 859, 964, 1452
HpyF10VI GCNNNNNNNGC 8 cut(s) 160, 174, 610, 758, 843, 865, 874, 1458
HpyF3I CTNAG 3 cut(s) 258, 921, 1295
HpySE526I ACGT 1 cut(s) 69
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 3 cut(s) 118, 946, 1282
Kpn2I TCCGGA 2 cut(s) 446, 1414
Kzo9I GATC 7 cut(s) 674, 946, 987, 1085, 1138, 1222, 1390
LguI GCTCTTC 1 cut(s) 594
LmnI GCTCC 7 cut(s) 668, 688, 874, 982, 1197, 1200, 1339
Lsp1109I GCAGC 5 cut(s) 719, 736, 758, 821, 843
LweI GCATC 3 cut(s) 361, 613, 886
MaeI CTAG 6 cut(s) 330, 678, 1187, 1196, 1317, 1465
MaeII ACGT 1 cut(s) 69
MaeIII GTNAC 2 cut(s) 233, 266
MalI GATC 7 cut(s) 676, 948, 989, 1087, 1140, 1224, 1392
MbiI CCGCTC 1 cut(s) 143
MboI GATC 7 cut(s) 674, 946, 987, 1085, 1138, 1222, 1390
MboII GAAGA 9 cut(s) 52, 611, 1239, 1265, 1364, 1375, 1381, 1414, 1420
MfeI CAATTG 1 cut(s) 1326
MflI RGATCY 2 cut(s) 946, 987
MhlI GDGCHC 4 cut(s) 264, 337, 685, 1194
MluCI AATT 3 cut(s) 83, 395, 1326
MlyI GAGTC 4 cut(s) 30, 109, 1123, 1253
MmeI TCCRAC 3 cut(s) 227, 710, 1157
MroI TCCGGA 2 cut(s) 446, 1414
MseI TTAA 1 cut(s) 668
MslI CAYNNNNRTG 1 cut(s) 1427
MspA1I CMGCKG 3 cut(s) 184, 710, 1146
MspR9I CCNGG 8 cut(s) 253, 290, 472, 473, 499, 525, 1066, 1267
MunI CAATTG 1 cut(s) 1326
Mva1269I GAATGC 1 cut(s) 555
MvaI CCWGG 3 cut(s) 499, 525, 1066
MwoI GCNNNNNNNGC 8 cut(s) 160, 174, 610, 758, 843, 865, 874, 1458
NciI CCSGG 5 cut(s) 253, 290, 472, 473, 1267
NcoI CCATGG 1 cut(s) 114
NdeII GATC 7 cut(s) 674, 946, 987, 1085, 1138, 1222, 1390
NlaIII CATG 3 cut(s) 118, 946, 1282
NlaIV GGNNCC 6 cut(s) 201, 327, 454, 631, 684, 948
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 1 cut(s) 266
PciSI GCTCTTC 1 cut(s) 594
PctI GAATGC 1 cut(s) 555
PfeI GAWTC 3 cut(s) 482, 754, 1411
PflFI GACNNNGTC 1 cut(s) 71
PflMI CCANNNNNTGG 2 cut(s) 425, 943
PfoI TCCNGGA 1 cut(s) 251
PinAI ACCGGT 2 cut(s) 206, 455
PkrI GCNGC 8 cut(s) 142, 709, 726, 751, 773, 798, 836, 858
PleI GAGTC 4 cut(s) 30, 108, 1123, 1252
PpsI GAGTC 4 cut(s) 30, 108, 1123, 1252
Psp124BI GAGCTC 2 cut(s) 264, 337
Psp6I CCWGG 3 cut(s) 497, 523, 1064
PspGI CCWGG 3 cut(s) 497, 523, 1064
PspN4I GGNNCC 6 cut(s) 201, 327, 454, 631, 684, 948
PspPI GGNCC 1 cut(s) 416
PsrI GAACNNNNNNTAC 2 cut(s) 1291, 1323
PsuI RGATCY 2 cut(s) 946, 987
PsyI GACNNNGTC 1 cut(s) 71
PvuII CAGCTG 1 cut(s) 710
RsaI GTAC 1 cut(s) 1457
RsaNI GTAC 1 cut(s) 1456
RseI CAYNNNNRTG 1 cut(s) 1427
SacI GAGCTC 2 cut(s) 264, 337
SapI GCTCTTC 1 cut(s) 594
SaqAI TTAA 1 cut(s) 668
SatI GCNGC 8 cut(s) 141, 708, 725, 750, 772, 797, 835, 857
Sau3AI GATC 7 cut(s) 674, 946, 987, 1085, 1138, 1222, 1390
Sau96I GGNCC 1 cut(s) 416
ScaI AGTACT 1 cut(s) 1457
SchI GAGTC 4 cut(s) 30, 109, 1123, 1253
ScrFI CCNGG 8 cut(s) 253, 290, 472, 473, 499, 525, 1066, 1267
SduI GDGCHC 4 cut(s) 264, 337, 685, 1194
SfaNI GCATC 3 cut(s) 361, 613, 886
SgrAI CRCCGGYG 1 cut(s) 455
SmaI CCCGGG 1 cut(s) 473
SmiMI CAYNNNNRTG 1 cut(s) 1427
SmlI CTYRAG 1 cut(s) 1131
SmoI CTYRAG 1 cut(s) 1131
SpeI ACTAGT 1 cut(s) 1186
Sse9I AATT 3 cut(s) 83, 395, 1326
SspMI CTAG 6 cut(s) 330, 678, 1187, 1196, 1317, 1465
SstI GAGCTC 2 cut(s) 264, 337
StyD4I CCNGG 8 cut(s) 251, 288, 470, 471, 497, 523, 1064, 1265
StyI CCWWGG 2 cut(s) 114, 1195
TaaI ACNGT 1 cut(s) 629
TaiI ACGT 1 cut(s) 72
TaqI TCGA 5 cut(s) 34, 639, 673, 1088, 1098
TasI AATT 3 cut(s) 83, 395, 1326
TatI WGTACW 1 cut(s) 1455
TauI GCSGC 3 cut(s) 143, 727, 799
TfiI GAWTC 3 cut(s) 482, 754, 1411
Tru1I TTAA 1 cut(s) 668
Tru9I TTAA 1 cut(s) 668
TscAI CASTG 2 cut(s) 1177, 1348
TseFI GTSAC 1 cut(s) 266
TseI GCWGC 5 cut(s) 707, 749, 771, 834, 856
Tsp45I GTSAC 1 cut(s) 266
TspDTI ATGAA 4 cut(s) 590, 1035, 1044, 1421
TspGWI ACGGA 1 cut(s) 93
TspMI CCCGGG 1 cut(s) 471
TspRI CASTG 2 cut(s) 1177, 1348
Tth111I GACNNNGTC 1 cut(s) 71
Van91I CCANNNNNTGG 2 cut(s) 425, 943
XapI RAATTY 1 cut(s) 83
XmaI CCCGGG 1 cut(s) 471
XmaJI CCTAGG 1 cut(s) 1195
XmiI GTMKAC 2 cut(s) 172, 1074
XspI CTAG 6 cut(s) 330, 678, 1187, 1196, 1317, 1465
ZraI GACGTC 1 cut(s) 70
ZrmI AGTACT 1 cut(s) 1457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.