FvH4_6g26910

GTP-binding protein TypA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005874
Physical Location & Seq
Reverse (-)
20580886 .. 20596784
15899 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g26910.t3

Sequence Viewer

Length: 2001 bp
ATGGCGGGGCCGTTGCTTCGCTCTCTCTGGTCCTCCTCCACGCGCAACACTCTCTCTTCCTCTCCGTCGAAACCCTACCTCCTCCGTCCCTCCGCCATCGCCCGCGCCTTCTCGGCGGCCACCGCGGCCTCCGATGCAACTCTTCAGAGCAGCTTGCTGGACCCGGCCCGCCTCCGCAACGTGGCGGTCATCGCTCACGTTGACCACGGCAAGACCACGCTGATGGACCGCCTCCTCCGCCAGTGCGGCGCCGACATCCCCCACGAACGCGCCATGGATTCCATCAGCCTCGAGCGCGAGCGCGGCATCACTATCGCCTCCAAGGTTACTTCGATTTTCTGGAAGGAGAATGAGTTAAACATGGTTGATACGCCTGGACATGCCGACTTTGGTGGAGAAGTTGAGCGGGTCGTTGGTATGGTGGAAGGTGCAGTTTTAGTTGTTGATGCCGGGGAAGGTCCGCTTGCACAGACTAAGTTTGTACTTGCCAAAGCCTTGAAATACGGGCTGCGTCCTATTCTTCTTCTGAACAAAGTAGATCGGCCTGCAGTTACTGAGGAGATGTGCGATGAAGTTGAGAGTCTGGTATTTGATCTTTTTGCAAATCTTGGTGCTACAGAGGAACAGCTAGATTTTCCTGTGCTATATGCTTCTGCTAAAGAAGGATGGGCTTCATCTACTTTCACTAAAGAACCTCCTGCTGATTCAAGAAACATGTCACAGCTACTTGATGCTATTATCAGACATGTTCCCCCACCAAATGCAAACATCGAGGCACCTTTCCAAATGCTGGTTTCCATGATGGAAAAGGACTTCTACCTTGGGCGAATACTGACTGGACGGGTTTCTTCTGGCTGCGTTCGCGTTGGTGACAGAGTTCATGGTCTTCGTAACAAAGATTCTGGGGTTGAGAAAATTGAAGAAGGGAAGGTTGTGAAACTGATGAAAAAGAAGGGAACAAATATGGTTCTCATTGACAGTGCTGGAGCTGGTGACATAATATCAATGGCTGGGTTAGCGAGTCCATCTATTGGCCATACAGTCACTAGCGTTGAGGTAATGACTGCATTACCAACTGTGGAGTTGGATCCTCCAACTATTTCCATGACTTTTGGTGTCAATGACTCTCCATTAGCTGGTCGTGATGGTACCCATCTGACCGGAGGAAAGATTGGAGATAGGCTATCAGCTGAAGCAGAAACAAATCTTGCCATTAATGTCCTCCCAGGCTTATCAGAGTCATTCGAAGTTCAGGGGAGGGGAGAACTTCAACTTGGTATCTTAATTGAGAATATGAGGCGTGAAGGATTCGAGCTCTCTGTGTCACCACCCAAAGTGATGTATAAAACAGAGAATGGTCAAAGGCTCGAGCCAATTGAAGAAGTTACTATAGAGGTAAATGAAGATCATGTGGGGTTAGTTATGGAAGCCTTATCTCATAGGCGAGCTGAGGTTCTTGACATGGGTCCTGTTCCAGGAAATGATGGCAGGACTAGATTGTCTTTGACATGTCCATCCAGGGGACTAGTTGGTTACAGAAGTGTATTTAGCAGTGCCACACGTGGAACTGGGTTTATGCATCGTGCTTTCTTAACGTATGAGAAATTTCGAGGTCCTCTTGGAAATGTGCGGAAAGGGGTTTTGGTGTCGATGGGCTATGGTACAATCACATCCTATGCATTAATGAGTTTAGAACCTCGTGGAACTCTATTTGTGACACCTGGGATGGAGACATATGATGGCATGATCGTTGGTGAACATTCGAGGGAGACAGATCTAGATGTCAACCCAGTAAGGAATAAGGAACTTACAAATATTCGTTCTGCTGGCAAGGATGAGAATGTGAAACTATCTCCTCCCCGCCTTATGACTCTTGAAGAAGCCATCGGGTATGTGGCCTCAGATGAGCTCATCGAGGTTACACCAAAGTTCATCCGGTTAAGGAAGAAATATCTGGATGTCAACAAGCGTAAAGCCATGAGCAAAAGGCAAAAGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFG_C GTP_EFTU GTP_EFTU_D2

Protein Analysis

667

Amino Acids

72.6

Weight (kDa)

6.15

Isoelectric Point (pI)

43.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 57 - 252 3.9e-53 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 260 - 350 1.3e-07 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 275 - 349 1.7e-08 Elongation factor Tu domain 2
EFG_C PF00679 456 - 538 1e-19 Elongation factor G C-terminus
BipA_C PF21018 545 - 653 1.8e-44 TypA/BipA C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1498
Acc65I GGTACC 1 cut(s) 1148
AccB1I GGYRCC 3 cut(s) 248, 775, 1148
AccB7I CCANNNNNTGG 3 cut(s) 790, 1031, 1136
AccBSI CCGCTC 1 cut(s) 406
AccII CGCG 7 cut(s) 43, 105, 125, 270, 297, 303, 864
AclWI GGATC 2 cut(s) 1082, 1095
AcoI YGGCCR 2 cut(s) 117, 1033
AcsI RAATTY 1 cut(s) 1604
AcuI CTGAAG 2 cut(s) 128, 1212
AcvI CACGTG 1 cut(s) 1562
AcyI GRCGYC 1 cut(s) 249
AfaI GTAC 3 cut(s) 483, 1150, 1663
AfiI CCNNNNNNNGG 6 cut(s) 181, 790, 1031, 1136, 1475, 1520
AflIII ACRYGT 4 cut(s) 714, 745, 1508, 1559
AgsI TTSAA 6 cut(s) 499, 708, 920, 1271, 1379, 1877
AhlI ACTAGT 1 cut(s) 1525
AjnI CCWGG 5 cut(s) 373, 1225, 1474, 1517, 1720
AjuI GAANNNNNNNTTGG 4 cut(s) 1257, 1289, 1625, 1657
AluBI AGCT 9 cut(s) 153, 628, 724, 989, 1136, 1190, 1315, 1448, 1909
AluI AGCT 9 cut(s) 153, 628, 724, 989, 1136, 1190, 1315, 1448, 1909
Alw21I GWGCWC 2 cut(s) 1317, 1911
Alw26I GTCTC 2 cut(s) 1724, 1763
AlwI GGATC 2 cut(s) 1082, 1095
AlwNI CAGNNNCTG 1 cut(s) 554
Ama87I CYCGRG 2 cut(s) 290, 1367
AoxI GGCC 7 cut(s) 8, 117, 126, 165, 542, 1033, 1896
ApeKI GCWGC 3 cut(s) 150, 508, 855
ApoI RAATTY 1 cut(s) 1604
AseI ATTAAT 2 cut(s) 1215, 1682
Asp718I GGTACC 1 cut(s) 1148
AspLEI GCGC 6 cut(s) 45, 107, 251, 272, 297, 303
AspS9I GGNCC 8 cut(s) 8, 30, 160, 166, 226, 458, 1466, 1613
AsuC2I CCSGG 2 cut(s) 164, 451
AsuHPI GGTGA 4 cut(s) 881, 1004, 1317, 1766
AsuII TTCGAA 1 cut(s) 1245
AvaI CYCGRG 2 cut(s) 290, 1367
AvaII GGWCC 6 cut(s) 30, 160, 226, 458, 1466, 1613
BalI TGGCCA 1 cut(s) 1035
BamHI GGATCC 1 cut(s) 1087
BanI GGYRCC 3 cut(s) 248, 775, 1148
BanII GRGCYC 2 cut(s) 1317, 1911
BauI CACGAG 1 cut(s) 1698
BbrPI CACGTG 1 cut(s) 1562
BbsI GAAGAC 1 cut(s) 878
Bbv12I GWGCWC 2 cut(s) 1317, 1911
BbvCI CCTCAGC 1 cut(s) 1449
BbvI GCAGC 3 cut(s) 162, 495, 842
BceAI ACGGC 1 cut(s) 223
BcgI CGANNNNNNTGC 2 cut(s) 295, 329
BciT130I CCWGG 5 cut(s) 375, 1227, 1476, 1519, 1722
BcnI CCSGG 2 cut(s) 164, 451
BcoDI GTCTC 2 cut(s) 1724, 1763
BcuI ACTAGT 1 cut(s) 1525
BfaI CTAG 5 cut(s) 629, 1047, 1494, 1526, 1778
BfmI CTRYAG 3 cut(s) 546, 615, 1389
BfoI RGCGCY 1 cut(s) 252
BglI GCCNNNNNGGC 3 cut(s) 113, 125, 246
BglII AGATCT 1 cut(s) 1774
BisI GCNGC 7 cut(s) 117, 126, 151, 247, 304, 509, 856
BlsI GCNGC 7 cut(s) 118, 127, 152, 248, 305, 510, 857
Bme1390I CCNGG 7 cut(s) 164, 375, 451, 1227, 1476, 1519, 1722
Bme18I GGWCC 6 cut(s) 30, 160, 226, 458, 1466, 1613
BmeT110I CYCGRG 2 cut(s) 290, 1367
BmgT120I GGNCC 8 cut(s) 8, 30, 160, 166, 226, 458, 1466, 1613
BmiI GGNNCC 7 cut(s) 9, 162, 250, 777, 1089, 1150, 1467
BmrFI CCNGG 7 cut(s) 164, 375, 451, 1227, 1476, 1519, 1722
BmrI ACTGGG 2 cut(s) 1578, 1784
BmsI GCATC 5 cut(s) 124, 315, 436, 721, 1588
BmuI ACTGGG 2 cut(s) 1578, 1784
BoxI GACNNNNGTC 1 cut(s) 1464
BpiI GAAGAC 1 cut(s) 878
BpmI CTGGAG 1 cut(s) 1005
Bpu10I CCTNAGC 1 cut(s) 1449
Bpu14I TTCGAA 1 cut(s) 1245
BpuMI CCSGG 2 cut(s) 164, 451
BsaAI YACGTR 1 cut(s) 1562
BsaHI GRCGYC 1 cut(s) 249
BsaJI CCNNGG 9 cut(s) 123, 205, 273, 321, 450, 820, 1225, 1518, 1721
BsaWI WCCGGW 2 cut(s) 1160, 1935
BsaXI ACNNNNNCTCC 4 cut(s) 63, 93, 338, 368
Bsc4I CCNNNNNNNGG 6 cut(s) 181, 790, 1031, 1136, 1475, 1520
Bse1I ACTGG 4 cut(s) 241, 841, 1573, 1790
BseBI CCWGG 5 cut(s) 375, 1227, 1476, 1519, 1722
BseDI CCNNGG 9 cut(s) 123, 205, 273, 321, 450, 820, 1225, 1518, 1721
BseGI GGATG 8 cut(s) 255, 671, 1514, 1670, 1731, 1840, 1932, 1963
BseLI CCNNNNNNNGG 6 cut(s) 181, 790, 1031, 1136, 1475, 1520
BseMII CTCAG 3 cut(s) 546, 1440, 1914
BseNI ACTGG 4 cut(s) 241, 841, 1573, 1790
BseRI GAGGAG 5 cut(s) 25, 71, 224, 572, 1845
BseXI GCAGC 3 cut(s) 162, 495, 842
BseYI CCCAGC 1 cut(s) 1010
BsgI GTGCAG 1 cut(s) 450
Bsh1236I CGCG 7 cut(s) 43, 105, 125, 270, 297, 303, 864
BshFI GGCC 7 cut(s) 10, 119, 128, 167, 544, 1035, 1898
BshNI GGYRCC 3 cut(s) 248, 775, 1148
BsiHKAI GWGCWC 2 cut(s) 1317, 1911
BsiHKCI CYCGRG 2 cut(s) 290, 1367
BsiSI CCGG 4 cut(s) 164, 450, 1161, 1936
BslFI GGGAC 2 cut(s) 72, 1536
BslI CCNNNNNNNGG 6 cut(s) 181, 790, 1031, 1136, 1475, 1520
BsmAI GTCTC 2 cut(s) 1724, 1763
BsmFI GGGAC 2 cut(s) 72, 1536
BsnI GGCC 7 cut(s) 10, 119, 128, 167, 544, 1035, 1898
BsoBI CYCGRG 2 cut(s) 290, 1367
Bsp119I TTCGAA 1 cut(s) 1245
Bsp1286I GDGCHC 2 cut(s) 1317, 1911
Bsp143I GATC 6 cut(s) 538, 592, 1087, 1405, 1746, 1774
Bsp19I CCATGG 1 cut(s) 273
BspANI GGCC 7 cut(s) 10, 119, 128, 167, 544, 1035, 1898
BspCNI CTCAG 3 cut(s) 547, 1441, 1913
BspFNI CGCG 7 cut(s) 43, 105, 125, 270, 297, 303, 864
BspLI GGNNCC 7 cut(s) 9, 162, 250, 777, 1089, 1150, 1467
BspMAI CTGCAG 1 cut(s) 550
BspPI GGATC 2 cut(s) 1082, 1095
BspT104I TTCGAA 1 cut(s) 1245
BspT107I GGYRCC 3 cut(s) 248, 775, 1148
BsrBI CCGCTC 1 cut(s) 406
BsrI ACTGG 4 cut(s) 241, 841, 1573, 1790
BssECI CCNNGG 9 cut(s) 123, 205, 273, 321, 450, 820, 1225, 1518, 1721
BssMI GATC 6 cut(s) 538, 592, 1087, 1405, 1746, 1774
BssNI GRCGYC 1 cut(s) 249
BssSI CACGAG 1 cut(s) 1698
BssT1I CCWWGG 3 cut(s) 273, 321, 820
Bst2BI CACGAG 1 cut(s) 1698
Bst2UI CCWGG 5 cut(s) 375, 1227, 1476, 1519, 1722
Bst4CI ACNGT 3 cut(s) 980, 1042, 1078
Bst6I CTCTTC 2 cut(s) 61, 147
BstACI GRCGYC 1 cut(s) 249
BstBAI YACGTR 1 cut(s) 1562
BstBI TTCGAA 1 cut(s) 1245
BstC8I GCNNGC 8 cut(s) 103, 155, 169, 299, 465, 546, 1446, 1828
BstDEI CTNAG 4 cut(s) 474, 555, 1449, 1900
BstDSI CCRYGG 3 cut(s) 123, 205, 273
BstENI CCTNNNNNAGG 1 cut(s) 1473
BstF5I GGATG 8 cut(s) 255, 671, 1514, 1670, 1731, 1840, 1932, 1963
BstFNI CGCG 7 cut(s) 43, 105, 125, 270, 297, 303, 864
BstH2I RGCGCY 1 cut(s) 252
BstHHI GCGC 6 cut(s) 45, 107, 251, 272, 297, 303
BstKTI GATC 6 cut(s) 541, 595, 1090, 1408, 1749, 1777
BstMAI GTCTC 2 cut(s) 1724, 1763
BstMBI GATC 6 cut(s) 538, 592, 1087, 1405, 1746, 1774
BstNI CCWGG 5 cut(s) 375, 1227, 1476, 1519, 1722
BstNSI RCATGY 4 cut(s) 383, 718, 749, 1512
BstPAI GACNNNNGTC 1 cut(s) 1464
BstSCI CCNGG 7 cut(s) 162, 373, 449, 1225, 1474, 1517, 1720
BstSFI CTRYAG 3 cut(s) 546, 615, 1389
BstUI CGCG 7 cut(s) 43, 105, 125, 270, 297, 303, 864
BstV1I GCAGC 3 cut(s) 162, 495, 842
BstV2I GAAGAC 1 cut(s) 878
BstX2I RGATCY 2 cut(s) 1087, 1774
BstXI CCANNNNNNTGG 1 cut(s) 223
BstYI RGATCY 2 cut(s) 1087, 1774
BsuRI GGCC 7 cut(s) 10, 119, 128, 167, 544, 1035, 1898
BtgI CCRYGG 3 cut(s) 123, 205, 273
BtgZI GCGATG 3 cut(s) 82, 175, 582
BtsCI GGATG 8 cut(s) 255, 671, 1514, 1670, 1731, 1840, 1932, 1963
BtsI GCAGTG 1 cut(s) 1558
BtsIMutI CAGTG 3 cut(s) 248, 985, 1558
Cac8I GCNNGC 8 cut(s) 103, 155, 169, 299, 465, 546, 1446, 1828
CaiI CAGNNNCTG 1 cut(s) 554
CfoI GCGC 6 cut(s) 45, 107, 251, 272, 297, 303
Cfr13I GGNCC 8 cut(s) 8, 30, 160, 166, 226, 458, 1466, 1613
Cfr42I CCGCGG 1 cut(s) 126
CseI GACGC 1 cut(s) 500
Csp6I GTAC 3 cut(s) 482, 1149, 1662
CviQI GTAC 3 cut(s) 482, 1149, 1662
DdeI CTNAG 4 cut(s) 474, 555, 1449, 1900
DinI GGCGCC 1 cut(s) 250
DpnI GATC 6 cut(s) 540, 594, 1089, 1407, 1748, 1776
DpnII GATC 6 cut(s) 538, 592, 1087, 1405, 1746, 1774
DrdI GACNNNNNNGTC 1 cut(s) 1498
DseDI GACNNNNNNGTC 1 cut(s) 1498
EaeI YGGCCR 2 cut(s) 117, 1033
Eam1104I CTCTTC 2 cut(s) 61, 147
EarI CTCTTC 2 cut(s) 61, 147
EciI GGCGGA 2 cut(s) 82, 227
Ecl136II GAGCTC 2 cut(s) 1315, 1909
Eco130I CCWWGG 3 cut(s) 273, 321, 820
Eco24I GRGCYC 2 cut(s) 1317, 1911
Eco47I GGWCC 6 cut(s) 30, 160, 226, 458, 1466, 1613
Eco53kI GAGCTC 2 cut(s) 1315, 1909
Eco57I CTGAAG 2 cut(s) 128, 1212
Eco72I CACGTG 1 cut(s) 1562
Eco88I CYCGRG 2 cut(s) 290, 1367
EcoICRI GAGCTC 2 cut(s) 1315, 1909
EcoNI CCTNNNNNAGG 1 cut(s) 1473
EcoO109I RGGNCCY 2 cut(s) 1466, 1613
EcoRII CCWGG 5 cut(s) 373, 1225, 1474, 1517, 1720
EcoT14I CCWWGG 3 cut(s) 273, 321, 820
EcoT22I ATGCAT 2 cut(s) 1581, 1681
EcoT38I GRGCYC 2 cut(s) 1317, 1911
EgeI GGCGCC 1 cut(s) 250
EheI GGCGCC 1 cut(s) 250
ErhI CCWWGG 3 cut(s) 273, 321, 820
FalI AAGNNNNNCTT 2 cut(s) 447, 479
FaqI GGGAC 2 cut(s) 72, 1536
FauI CCCGC 4 cut(s) 110, 176, 399, 1868
FauNDI CATATG 1 cut(s) 1735
Fnu4HI GCNGC 7 cut(s) 117, 126, 151, 247, 304, 509, 856
FokI GGATG 8 cut(s) 242, 678, 1501, 1657, 1738, 1847, 1919, 1970
FriOI GRGCYC 2 cut(s) 1317, 1911
Fsp4HI GCNGC 7 cut(s) 117, 126, 151, 247, 304, 509, 856
FspBI CTAG 5 cut(s) 629, 1047, 1494, 1526, 1778
GlaI GCGC 6 cut(s) 44, 106, 250, 271, 296, 302
GluI GCNGC 7 cut(s) 117, 126, 151, 247, 304, 509, 856
GsaI CCCAGC 1 cut(s) 1014
GsuI CTGGAG 1 cut(s) 1005
HaeII RGCGCY 1 cut(s) 252
HaeIII GGCC 7 cut(s) 10, 119, 128, 167, 544, 1035, 1898
HapII CCGG 4 cut(s) 164, 450, 1161, 1936
HgaI GACGC 1 cut(s) 500
HhaI GCGC 6 cut(s) 45, 107, 251, 272, 297, 303
Hin1I GRCGYC 1 cut(s) 249
Hin6I GCGC 6 cut(s) 43, 105, 249, 270, 295, 301
HinP1I GCGC 6 cut(s) 43, 105, 249, 270, 295, 301
HincII GTYRAC 3 cut(s) 202, 1786, 1963
HindII GTYRAC 3 cut(s) 202, 1786, 1963
HinfI GANTC 9 cut(s) 278, 580, 704, 899, 1021, 1124, 1238, 1308, 1870
HpaII CCGG 4 cut(s) 164, 450, 1161, 1936
HphI GGTGA 4 cut(s) 881, 1004, 1317, 1766
Hpy166II GTNNAC 4 cut(s) 202, 1757, 1786, 1963
Hpy188I TCNGA 7 cut(s) 133, 147, 528, 743, 1158, 1237, 1903
Hpy188III TCNNGA 7 cut(s) 340, 708, 1142, 1457, 1778, 1874, 1955
Hpy8I GTNNAC 4 cut(s) 202, 1757, 1786, 1963
Hpy99I CGWCG 1 cut(s) 70
HpyAV CCTTC 9 cut(s) 118, 337, 419, 449, 656, 917, 922, 946, 1298
HpyCH4III ACNGT 3 cut(s) 980, 1042, 1078
HpyCH4IV ACGT 4 cut(s) 180, 198, 1561, 1595
HpyCH4V TGCA 9 cut(s) 137, 431, 467, 548, 602, 764, 1067, 1579, 1679
HpyF3I CTNAG 4 cut(s) 474, 555, 1449, 1900
HpySE526I ACGT 4 cut(s) 180, 198, 1561, 1595
Hsp92I GRCGYC 1 cut(s) 249
HspAI GCGC 6 cut(s) 43, 105, 249, 270, 295, 301
KasI GGCGCC 1 cut(s) 248
KpnI GGTACC 1 cut(s) 1152
KspI CCGCGG 1 cut(s) 126
Kzo9I GATC 6 cut(s) 538, 592, 1087, 1405, 1746, 1774
LmnI GCTCC 1 cut(s) 986
Lsp1109I GCAGC 3 cut(s) 162, 495, 842
LweI GCATC 5 cut(s) 124, 315, 436, 721, 1588
MaeI CTAG 5 cut(s) 629, 1047, 1494, 1526, 1778
MaeII ACGT 4 cut(s) 180, 198, 1561, 1595
MalI GATC 6 cut(s) 540, 594, 1089, 1407, 1748, 1776
MbiI CCGCTC 1 cut(s) 406
MboI GATC 6 cut(s) 538, 592, 1087, 1405, 1746, 1774
MfeI CAATTG 1 cut(s) 1374
MflI RGATCY 2 cut(s) 1087, 1774
MhlI GDGCHC 2 cut(s) 1317, 1911
MlsI TGGCCA 1 cut(s) 1035
MluCI AATT 4 cut(s) 915, 1284, 1374, 1604
MluNI TGGCCA 1 cut(s) 1035
Mly113I GGCGCC 1 cut(s) 249
MlyI GAGTC 5 cut(s) 589, 1030, 1118, 1247, 1864
MmeI TCCRAC 2 cut(s) 1065, 1118
Mox20I TGGCCA 1 cut(s) 1035
Mph1103I ATGCAT 2 cut(s) 1581, 1681
MscI TGGCCA 1 cut(s) 1035
MseI TTAA 6 cut(s) 356, 1215, 1283, 1592, 1682, 1940
MslI CAYNNNNRTG 1 cut(s) 221
Msp20I TGGCCA 1 cut(s) 1035
MspA1I CMGCKG 2 cut(s) 125, 1190
MspI CCGG 4 cut(s) 164, 450, 1161, 1936
MspR9I CCNGG 7 cut(s) 164, 375, 451, 1227, 1476, 1519, 1722
MunI CAATTG 1 cut(s) 1374
MvaI CCWGG 5 cut(s) 375, 1227, 1476, 1519, 1722
MvnI CGCG 7 cut(s) 43, 105, 125, 270, 297, 303, 864
NarI GGCGCC 1 cut(s) 249
NciI CCSGG 2 cut(s) 164, 451
NcoI CCATGG 1 cut(s) 273
NdeI CATATG 1 cut(s) 1735
NdeII GATC 6 cut(s) 538, 592, 1087, 1405, 1746, 1774
NlaIV GGNNCC 7 cut(s) 9, 162, 250, 777, 1089, 1150, 1467
NmeAIII GCCGAG 1 cut(s) 92
NmuCI GTSAC 6 cut(s) 717, 869, 992, 1042, 1323, 1714
NsiI ATGCAT 2 cut(s) 1581, 1681
NspI RCATGY 4 cut(s) 383, 718, 749, 1512
NspV TTCGAA 1 cut(s) 1245
PaeR7I CTCGAG 2 cut(s) 290, 1367
PciI ACATGT 3 cut(s) 714, 745, 1508
PfeI GAWTC 4 cut(s) 278, 704, 899, 1308
PflMI CCANNNNNTGG 3 cut(s) 790, 1031, 1136
PfoI TCCNGGA 1 cut(s) 1474
PkrI GCNGC 7 cut(s) 118, 127, 152, 248, 305, 510, 857
PleI GAGTC 5 cut(s) 588, 1029, 1118, 1246, 1864
PluTI GGCGCC 1 cut(s) 252
PmaCI CACGTG 1 cut(s) 1562
PmlI CACGTG 1 cut(s) 1562
PpsI GAGTC 5 cut(s) 588, 1029, 1118, 1246, 1864
Ppu21I YACGTR 1 cut(s) 1562
PpuMI RGGWCCY 2 cut(s) 1466, 1613
PscI ACATGT 3 cut(s) 714, 745, 1508
PshAI GACNNNNGTC 1 cut(s) 1464
PshBI ATTAAT 2 cut(s) 1215, 1682
Psp124BI GAGCTC 2 cut(s) 1317, 1911
Psp5II RGGWCCY 2 cut(s) 1466, 1613
Psp6I CCWGG 5 cut(s) 373, 1225, 1474, 1517, 1720
PspCI CACGTG 1 cut(s) 1562
PspFI CCCAGC 1 cut(s) 1010
PspGI CCWGG 5 cut(s) 373, 1225, 1474, 1517, 1720
PspN4I GGNNCC 7 cut(s) 9, 162, 250, 777, 1089, 1150, 1467
PspPI GGNCC 8 cut(s) 8, 30, 160, 166, 226, 458, 1466, 1613
PspPPI RGGWCCY 2 cut(s) 1466, 1613
PspXI VCTCGAGB 2 cut(s) 290, 1367
PstI CTGCAG 1 cut(s) 550
PstNI CAGNNNCTG 1 cut(s) 554
PsuI RGATCY 2 cut(s) 1087, 1774
PvuII CAGCTG 1 cut(s) 1190
RsaI GTAC 3 cut(s) 483, 1150, 1663
RsaNI GTAC 3 cut(s) 482, 1149, 1662
RseI CAYNNNNRTG 1 cut(s) 221
SacI GAGCTC 2 cut(s) 1317, 1911
SacII CCGCGG 1 cut(s) 126
SaqAI TTAA 6 cut(s) 356, 1215, 1283, 1592, 1682, 1940
SatI GCNGC 7 cut(s) 117, 126, 151, 247, 304, 509, 856
Sau3AI GATC 6 cut(s) 538, 592, 1087, 1405, 1746, 1774
Sau96I GGNCC 8 cut(s) 8, 30, 160, 166, 226, 458, 1466, 1613
SchI GAGTC 5 cut(s) 589, 1030, 1118, 1247, 1864
ScrFI CCNGG 7 cut(s) 164, 375, 451, 1227, 1476, 1519, 1722
SduI GDGCHC 2 cut(s) 1317, 1911
SfaNI GCATC 5 cut(s) 124, 315, 436, 721, 1588
SfcI CTRYAG 3 cut(s) 546, 615, 1389
SfiI GGCCNNNNNGGCC 1 cut(s) 125
SfoI GGCGCC 1 cut(s) 250
Sfr274I CTCGAG 2 cut(s) 290, 1367
Sfr303I CCGCGG 1 cut(s) 126
SfuI TTCGAA 1 cut(s) 1245
SgrBI CCGCGG 1 cut(s) 126
SinI GGWCC 6 cut(s) 30, 160, 226, 458, 1466, 1613
SlaI CTCGAG 2 cut(s) 290, 1367
SmiMI CAYNNNNRTG 1 cut(s) 221
SmlI CTYRAG 2 cut(s) 290, 1367
SmoI CTYRAG 2 cut(s) 290, 1367
SpeI ACTAGT 1 cut(s) 1525
Sse9I AATT 4 cut(s) 915, 1284, 1374, 1604
SspDI GGCGCC 1 cut(s) 248
SspI AATATT 1 cut(s) 1816
SspMI CTAG 5 cut(s) 629, 1047, 1494, 1526, 1778
SstI GAGCTC 2 cut(s) 1317, 1911
StyD4I CCNGG 7 cut(s) 162, 373, 449, 1225, 1474, 1517, 1720
StyI CCWWGG 3 cut(s) 273, 321, 820
TaaI ACNGT 3 cut(s) 980, 1042, 1078
TaiI ACGT 4 cut(s) 183, 201, 1564, 1598
TasI AATT 4 cut(s) 915, 1284, 1374, 1604
TatI WGTACW 1 cut(s) 481
TauI GCSGC 4 cut(s) 119, 128, 249, 306
TfiI GAWTC 4 cut(s) 278, 704, 899, 1308
Tru1I TTAA 6 cut(s) 356, 1215, 1283, 1592, 1682, 1940
Tru9I TTAA 6 cut(s) 356, 1215, 1283, 1592, 1682, 1940
TscAI CASTG 3 cut(s) 248, 985, 1558
TseFI GTSAC 6 cut(s) 717, 869, 992, 1042, 1323, 1714
TseI GCWGC 3 cut(s) 150, 508, 855
Tsp45I GTSAC 6 cut(s) 717, 869, 992, 1042, 1323, 1714
TspDTI ATGAA 6 cut(s) 585, 663, 869, 959, 1416, 1921
TspGWI ACGGA 2 cut(s) 54, 74
TspRI CASTG 3 cut(s) 248, 985, 1558
Van91I CCANNNNNTGG 3 cut(s) 790, 1031, 1136
VpaK11BI GGWCC 6 cut(s) 30, 160, 226, 458, 1466, 1613
VspI ATTAAT 2 cut(s) 1215, 1682
XagI CCTNNNNNAGG 1 cut(s) 1473
XapI RAATTY 1 cut(s) 1604
XbaI TCTAGA 1 cut(s) 1777
XceI RCATGY 4 cut(s) 383, 718, 749, 1512
XcmI CCANNNNNNNNNTGG 1 cut(s) 1891
XhoI CTCGAG 2 cut(s) 290, 1367
XspI CTAG 5 cut(s) 629, 1047, 1494, 1526, 1778
Zsp2I ATGCAT 2 cut(s) 1581, 1681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.