Rroxscaffold_2G00115890

GTP-binding protein TypA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005874
Physical Location & Seq
Forward (+)
42421017 .. 42435207
14191 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00115890.1

Sequence Viewer

Length: 2124 bp
ATGGCGGGGCCGTTGCTTCGCTCTCTCTGGTCCTCCACGCGTAACACTCTATCCTCTTCCTCCTCCAAACCCTACCTTCGTCCCTCCGCACTCGCCCGCACCTTCTCGGCGGCCACCGCGGCCACCGATGCTACTCTACAGAGGAGCAGCCTGCTCGACCCGGCCCGCCTCCGCAACGTGGCGGTCATAGCGCACGTTGACCACGGCAAGACCACCCTCATGGACCGCCTCCTCCGCCAGTGCGGCGCCGACATCCCCCACGAACGCGCCATGGATTCCATCAGCCTCGAGCGCGAGCGCGGCATCACTATCGCCTCCAAGGTTACGTCGGTTTTCTGGAAGGAGAATGAGCTAAACATGGTTGATACGCCTGGACATGCAGACTTTGGTGGTGAAGTTGAGCGGGTTGTCGGTATGGTCGAGGGAGCAGTTTTAGTTGTTGATGCCGGGGAAGGTCCGCTTGCACAGACCAAGTTTGTGCTTGCCAAAGCCTTAAAATACGGGCTGCGTCCTATTCTTCTTCTAAACAAAGTTGATCGGCCTGCAGTTTCTGAGGAGATGTGCGATGAAGTTGAGAGTCTGGTATTTGATCTTTTTGCAAATCTCGGTGCTACAGAGGAGCAGCTGGATTTTCCTGTGCTATATGCTTCTGCTAAAGAAGGATGGGCCTCGGCTACTTTCACTAAAGAACCTCCTGCTGATTCAAGAAACATGTCGCAGCTGCTTGATGCTATTATCAGACATGTTCCCCCACCAAGTGCAAACGTTGAGGCACCTTTCCAGATGCTGGTTTCCATGATGGAAAAGGACTTCTACCTTGGGCGAATATTGACTGGACGGGTTTCTTCTGGCTGTGTTCGCGTTGGTGATAGAGTTCATGGGCTTCGTAACAAAGATTCTGGAGTTGAGAAAATTGAAGAAGGGAAGGTTGTGAAACTGATGAAAAAGAAGGGAACAAATATGGTTCTCATTGACAGTGCTGGAGCTGGTGACATAATATCAATGGCTGGGTTAGCAAGTCCATCTATTGGCCATACAGTCACTAGCGTTGAGGCCTTTCCTGCTTTGAAGGCTTGGGGCGCCTACAAAGTTTTCTGGCTCTTAAGGTTTTCAGCTCTTGATACATGCATTCCTGCTAGTGGTTGTTGGCATTTAAAAGCAAGCATGGCTCACATGTGCGATGCTAAGCAGGTAATGACTGCATTACCAACCGTTGAGTTGGATCCACCAACTATTTCCATGACTTTTGGTGTAAATGACTCTCCATTAGCTGGCCGTGATGGTAGCCATCTGACCGGAGGAAAGATCGGGGATCGGTTATCAGCTGAAGCAGAAACAAATCTTGCTATTAATGTCCTCCCAGGCTTATCAGAGTCATTCGAAGTTCAGGGGAGGGGAGAACTGCAACTCGGTATATTAATTGAGAATATGAGGCGTGAAGGCTTTGAGCTCTCTGTGTCACCACCCAAAGTCATGTATAAAACAGAGAATGGTCAAAGGCTCGAGCCAATTGAAGAAGTAAATGAAGACCATGTGGGGTTAGTTATGGAAGCCTTATCTCATAGGCGAGCTGAGGTTCTTGACATGGGTCCTGTTCCAGGAAATGATGGCAGGACTAGATTGTCTTTGACATGTCCATCCAGGGGACTGGTTGGTTACAGAAGTGTATTTAGCAGTGCCACGCGTGGAACTGGGTTTATGCATCGTGCTTTCTTAACATATGAAAAATTTCGAGGTCCTCTTGGAAATGTGCGGAAAGGGGTTTTGGTGTCAATGGGCTATGGTACAATCACATCCTATGCATTAATGAGTTTAGAACCTCGTGGAACTCTATTTGTGACACCTGGTATGGAGACATATGATGGCATGATTGTTGGTGAACATTCGAGGGAGACGGATCTAGATGTCAACCCAGTAAGGAACAAAGAACTTACAAATATTCGTTCTGCTGGCAAGGATGAAAATGTGAAACTATCTCCTCCTCGCCTTATGACTCTTGAAGAAGCCATAGGGTATGTAGCCTCGGATGAGCTCATTGAGGTTACACCGAAGTCCATCCGGTTACGGAAGAAATATCTGGATGTCAACAAGCGTAAAACCATGAGCAAAAGGCAAAAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFG_C GTP_EFTU GTP_EFTU_D2

Protein Analysis

707

Amino Acids

77.15

Weight (kDa)

6.95

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 56 - 251 4.2e-53 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 259 - 349 1.5e-07 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 274 - 348 1.8e-08 Elongation factor Tu domain 2
EFG_C PF00679 501 - 579 4.3e-14 Elongation factor G C-terminus
BipA_C PF21018 586 - 694 3.7e-45 TypA/BipA C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1621
Acc36I ACCTGC 1 cut(s) 1180
AccB1I GGYRCC 3 cut(s) 245, 772, 1079
AccB7I CCANNNNNTGG 3 cut(s) 787, 1028, 1271
AccBSI CCGCTC 1 cut(s) 403
AccII CGCG 7 cut(s) 40, 119, 267, 294, 300, 861, 1684
AclI AACGTT 1 cut(s) 765
AclWI GGATC 4 cut(s) 1217, 1230, 1320, 1905
AcoI YGGCCR 4 cut(s) 111, 120, 1030, 1273
AcsI RAATTY 1 cut(s) 1727
AcuI CTGAAG 1 cut(s) 1347
AcyI GRCGYC 2 cut(s) 246, 1080
AdeI CACNNNGTG 1 cut(s) 758
AfaI GTAC 1 cut(s) 1786
AfiI CCNNNNNNNGG 7 cut(s) 178, 787, 1028, 1139, 1271, 1598, 1643
AflII CTTAAG 1 cut(s) 1102
AflIII ACRYGT 6 cut(s) 38, 711, 742, 1173, 1631, 1682
AgsI TTSAA 5 cut(s) 705, 917, 1069, 1514, 2000
AjnI CCWGG 5 cut(s) 370, 1360, 1597, 1640, 1843
AjuI GAANNNNNNNTTGG 2 cut(s) 1748, 1780
Alw21I GWGCWC 2 cut(s) 1452, 2034
Alw26I GTCTC 2 cut(s) 1847, 1886
AlwI GGATC 4 cut(s) 1217, 1230, 1320, 1905
AlwNI CAGNNNCTG 2 cut(s) 551, 787
Ama87I CYCGRG 2 cut(s) 287, 1502
AoxI GGCC 9 cut(s) 8, 111, 120, 162, 539, 666, 1030, 1053, 1273
ApeKI GCWGC 5 cut(s) 147, 505, 622, 718, 721
ApoI RAATTY 1 cut(s) 1727
AseI ATTAAT 3 cut(s) 1350, 1418, 1805
Asp700I GAANNNNTTC 1 cut(s) 1728
AspLEI GCGC 6 cut(s) 193, 248, 269, 294, 300, 1082
AspS9I GGNCC 8 cut(s) 8, 30, 163, 223, 455, 666, 1589, 1736
AsuC2I CCSGG 2 cut(s) 161, 448
AsuHPI GGTGA 5 cut(s) 404, 878, 1001, 1452, 1889
AsuII TTCGAA 1 cut(s) 1380
AvaI CYCGRG 2 cut(s) 287, 1502
AvaII GGWCC 5 cut(s) 30, 223, 455, 1589, 1736
BalI TGGCCA 1 cut(s) 1032
BamHI GGATCC 1 cut(s) 1222
BanI GGYRCC 3 cut(s) 245, 772, 1079
BanII GRGCYC 2 cut(s) 1452, 2034
BauI CACGAG 1 cut(s) 1821
BbsI GAAGAC 1 cut(s) 1533
Bbv12I GWGCWC 2 cut(s) 1452, 2034
BbvCI CCTCAGC 1 cut(s) 1572
BbvI GCAGC 5 cut(s) 159, 492, 634, 708, 730
BceAI ACGGC 2 cut(s) 220, 1260
BcgI CGANNNNNNTGC 4 cut(s) 136, 170, 292, 326
BciT130I CCWGG 5 cut(s) 372, 1362, 1599, 1642, 1845
BcnI CCSGG 2 cut(s) 161, 448
BcoDI GTCTC 2 cut(s) 1847, 1886
BfaI CTAG 4 cut(s) 1044, 1137, 1617, 1901
BfmI CTRYAG 3 cut(s) 137, 543, 612
BfoI RGCGCY 2 cut(s) 249, 1083
BfrI CTTAAG 1 cut(s) 1102
BfuAI ACCTGC 1 cut(s) 1180
BglI GCCNNNNNGGC 2 cut(s) 119, 243
BisI GCNGC 9 cut(s) 111, 120, 148, 244, 301, 506, 623, 719, 722
BlpI GCTNAGC 1 cut(s) 1185
BlsI GCNGC 9 cut(s) 112, 121, 149, 245, 302, 507, 624, 720, 723
Bme1390I CCNGG 7 cut(s) 161, 372, 448, 1362, 1599, 1642, 1845
Bme18I GGWCC 5 cut(s) 30, 223, 455, 1589, 1736
BmeT110I CYCGRG 2 cut(s) 287, 1502
BmgT120I GGNCC 8 cut(s) 8, 30, 163, 223, 455, 666, 1589, 1736
BmiI GGNNCC 6 cut(s) 9, 247, 774, 1081, 1224, 1590
BmrFI CCNGG 7 cut(s) 161, 372, 448, 1362, 1599, 1642, 1845
BmrI ACTGGG 2 cut(s) 1701, 1907
BmsI GCATC 7 cut(s) 118, 312, 433, 718, 774, 1171, 1711
BmuI ACTGGG 2 cut(s) 1701, 1907
BoxI GACNNNNGTC 1 cut(s) 1587
BpiI GAAGAC 1 cut(s) 1533
BpmI CTGGAG 2 cut(s) 921, 1002
Bpu10I CCTNAGC 1 cut(s) 1572
Bpu1102I GCTNAGC 1 cut(s) 1185
Bpu14I TTCGAA 1 cut(s) 1380
BpuMI CCSGG 2 cut(s) 161, 448
BsaHI GRCGYC 2 cut(s) 246, 1080
BsaWI WCCGGW 2 cut(s) 1295, 2058
Bsc4I CCNNNNNNNGG 7 cut(s) 178, 787, 1028, 1139, 1271, 1598, 1643
Bse1I ACTGG 5 cut(s) 238, 838, 1653, 1696, 1913
BseBI CCWGG 5 cut(s) 372, 1362, 1599, 1642, 1845
BseGI GGATG 8 cut(s) 252, 668, 1637, 1793, 1963, 2032, 2055, 2086
BseLI CCNNNNNNNGG 7 cut(s) 178, 787, 1028, 1139, 1271, 1598, 1643
BseMII CTCAG 2 cut(s) 543, 1563
BseNI ACTGG 5 cut(s) 238, 838, 1653, 1696, 1913
BseRI GAGGAG 7 cut(s) 52, 157, 221, 569, 632, 1968, 1971
BseXI GCAGC 5 cut(s) 159, 492, 634, 708, 730
BseYI CCCAGC 1 cut(s) 1007
Bsh1236I CGCG 7 cut(s) 40, 119, 267, 294, 300, 861, 1684
BshFI GGCC 9 cut(s) 10, 113, 122, 164, 541, 668, 1032, 1055, 1275
BshNI GGYRCC 3 cut(s) 245, 772, 1079
BsiHKAI GWGCWC 2 cut(s) 1452, 2034
BsiHKCI CYCGRG 2 cut(s) 287, 1502
BsiSI CCGG 4 cut(s) 161, 447, 1296, 2059
BslFI GGGAC 2 cut(s) 66, 1659
BslI CCNNNNNNNGG 7 cut(s) 178, 787, 1028, 1139, 1271, 1598, 1643
BsmAI GTCTC 2 cut(s) 1847, 1886
BsmBI CGTCTC 1 cut(s) 1886
BsmFI GGGAC 2 cut(s) 66, 1659
BsmI GAATGC 1 cut(s) 1128
BsnI GGCC 9 cut(s) 10, 113, 122, 164, 541, 668, 1032, 1055, 1275
BsoBI CYCGRG 2 cut(s) 287, 1502
Bsp119I TTCGAA 1 cut(s) 1380
Bsp1286I GDGCHC 2 cut(s) 1452, 2034
Bsp143I GATC 6 cut(s) 535, 589, 1222, 1305, 1312, 1897
Bsp1720I GCTNAGC 1 cut(s) 1185
Bsp19I CCATGG 1 cut(s) 270
BspANI GGCC 9 cut(s) 10, 113, 122, 164, 541, 668, 1032, 1055, 1275
BspCNI CTCAG 2 cut(s) 544, 1564
BspFNI CGCG 7 cut(s) 40, 119, 267, 294, 300, 861, 1684
BspLI GGNNCC 6 cut(s) 9, 247, 774, 1081, 1224, 1590
BspMAI CTGCAG 1 cut(s) 547
BspMI ACCTGC 1 cut(s) 1180
BspPI GGATC 4 cut(s) 1217, 1230, 1320, 1905
BspT104I TTCGAA 1 cut(s) 1380
BspT107I GGYRCC 3 cut(s) 245, 772, 1079
BspTI CTTAAG 1 cut(s) 1102
BsrBI CCGCTC 1 cut(s) 403
BsrI ACTGG 5 cut(s) 238, 838, 1653, 1696, 1913
BssMI GATC 6 cut(s) 535, 589, 1222, 1305, 1312, 1897
BssNI GRCGYC 2 cut(s) 246, 1080
BssSI CACGAG 1 cut(s) 1821
BssT1I CCWWGG 3 cut(s) 270, 318, 817
Bst2BI CACGAG 1 cut(s) 1821
Bst2UI CCWGG 5 cut(s) 372, 1362, 1599, 1642, 1845
Bst4CI ACNGT 3 cut(s) 977, 1039, 1213
Bst6I CTCTTC 1 cut(s) 61
BstACI GRCGYC 2 cut(s) 246, 1080
BstAFI CTTAAG 1 cut(s) 1102
BstBI TTCGAA 1 cut(s) 1380
BstDEI CTNAG 3 cut(s) 552, 1185, 1572
BstDSI CCRYGG 3 cut(s) 117, 202, 270
BstENI CCTNNNNNAGG 1 cut(s) 1596
BstF5I GGATG 8 cut(s) 252, 668, 1637, 1793, 1963, 2032, 2055, 2086
BstFNI CGCG 7 cut(s) 40, 119, 267, 294, 300, 861, 1684
BstH2I RGCGCY 2 cut(s) 249, 1083
BstHHI GCGC 6 cut(s) 193, 248, 269, 294, 300, 1082
BstKTI GATC 6 cut(s) 538, 592, 1225, 1308, 1315, 1900
BstMAI GTCTC 2 cut(s) 1847, 1886
BstMBI GATC 6 cut(s) 535, 589, 1222, 1305, 1312, 1897
BstNI CCWGG 5 cut(s) 372, 1362, 1599, 1642, 1845
BstNSI RCATGY 6 cut(s) 380, 715, 746, 1128, 1177, 1635
BstPAI GACNNNNGTC 1 cut(s) 1587
BstSCI CCNGG 7 cut(s) 159, 370, 446, 1360, 1597, 1640, 1843
BstSFI CTRYAG 3 cut(s) 137, 543, 612
BstUI CGCG 7 cut(s) 40, 119, 267, 294, 300, 861, 1684
BstV1I GCAGC 5 cut(s) 159, 492, 634, 708, 730
BstV2I GAAGAC 1 cut(s) 1533
BstX2I RGATCY 2 cut(s) 1222, 1897
BstXI CCANNNNNNTGG 2 cut(s) 220, 1648
BstYI RGATCY 2 cut(s) 1222, 1897
BsuRI GGCC 9 cut(s) 10, 113, 122, 164, 541, 668, 1032, 1055, 1275
BtgI CCRYGG 3 cut(s) 117, 202, 270
BtgZI GCGATG 2 cut(s) 579, 1194
BtsCI GGATG 8 cut(s) 252, 668, 1637, 1793, 1963, 2032, 2055, 2086
BtsI GCAGTG 1 cut(s) 1681
BtsIMutI CAGTG 3 cut(s) 245, 982, 1681
BveI ACCTGC 1 cut(s) 1180
CaiI CAGNNNCTG 2 cut(s) 551, 787
CfoI GCGC 6 cut(s) 193, 248, 269, 294, 300, 1082
Cfr13I GGNCC 8 cut(s) 8, 30, 163, 223, 455, 666, 1589, 1736
Cfr42I CCGCGG 1 cut(s) 120
CseI GACGC 1 cut(s) 497
CsiI ACCWGGT 1 cut(s) 1843
Csp6I GTAC 1 cut(s) 1785
CviQI GTAC 1 cut(s) 1785
DdeI CTNAG 3 cut(s) 552, 1185, 1572
DinI GGCGCC 2 cut(s) 247, 1081
DpnI GATC 6 cut(s) 537, 591, 1224, 1307, 1314, 1899
DpnII GATC 6 cut(s) 535, 589, 1222, 1305, 1312, 1897
DraI TTTAAA 1 cut(s) 1155
DraIII CACNNNGTG 1 cut(s) 758
DrdI GACNNNNNNGTC 1 cut(s) 1621
DseDI GACNNNNNNGTC 1 cut(s) 1621
EaeI YGGCCR 4 cut(s) 111, 120, 1030, 1273
Eam1104I CTCTTC 1 cut(s) 61
EarI CTCTTC 1 cut(s) 61
EciI GGCGGA 1 cut(s) 224
Ecl136II GAGCTC 2 cut(s) 1450, 2032
Eco130I CCWWGG 3 cut(s) 270, 318, 817
Eco147I AGGCCT 1 cut(s) 1055
Eco24I GRGCYC 2 cut(s) 1452, 2034
Eco47I GGWCC 5 cut(s) 30, 223, 455, 1589, 1736
Eco53kI GAGCTC 2 cut(s) 1450, 2032
Eco57I CTGAAG 1 cut(s) 1347
Eco88I CYCGRG 2 cut(s) 287, 1502
EcoICRI GAGCTC 2 cut(s) 1450, 2032
EcoNI CCTNNNNNAGG 1 cut(s) 1596
EcoO109I RGGNCCY 2 cut(s) 1589, 1736
EcoRII CCWGG 5 cut(s) 370, 1360, 1597, 1640, 1843
EcoT14I CCWWGG 3 cut(s) 270, 318, 817
EcoT22I ATGCAT 3 cut(s) 1130, 1704, 1804
EcoT38I GRGCYC 2 cut(s) 1452, 2034
EgeI GGCGCC 2 cut(s) 247, 1081
EheI GGCGCC 2 cut(s) 247, 1081
ErhI CCWWGG 3 cut(s) 270, 318, 817
Esp3I CGTCTC 1 cut(s) 1886
FalI AAGNNNNNCTT 2 cut(s) 444, 476
FaqI GGGAC 2 cut(s) 66, 1659
FauI CCCGC 3 cut(s) 104, 173, 396
FauNDI CATATG 2 cut(s) 1720, 1858
Fnu4HI GCNGC 9 cut(s) 111, 120, 148, 244, 301, 506, 623, 719, 722
FokI GGATG 8 cut(s) 239, 675, 1624, 1780, 1970, 2039, 2042, 2093
FriOI GRGCYC 2 cut(s) 1452, 2034
Fsp4HI GCNGC 9 cut(s) 111, 120, 148, 244, 301, 506, 623, 719, 722
FspBI CTAG 4 cut(s) 1044, 1137, 1617, 1901
GlaI GCGC 6 cut(s) 192, 247, 268, 293, 299, 1081
GluI GCNGC 9 cut(s) 111, 120, 148, 244, 301, 506, 623, 719, 722
GsaI CCCAGC 1 cut(s) 1011
GsuI CTGGAG 2 cut(s) 921, 1002
HaeII RGCGCY 2 cut(s) 249, 1083
HaeIII GGCC 9 cut(s) 10, 113, 122, 164, 541, 668, 1032, 1055, 1275
HapII CCGG 4 cut(s) 161, 447, 1296, 2059
HgaI GACGC 1 cut(s) 497
HhaI GCGC 6 cut(s) 193, 248, 269, 294, 300, 1082
Hin1I GRCGYC 2 cut(s) 246, 1080
Hin6I GCGC 6 cut(s) 191, 246, 267, 292, 298, 1080
HinP1I GCGC 6 cut(s) 191, 246, 267, 292, 298, 1080
HincII GTYRAC 3 cut(s) 199, 1909, 2086
HindII GTYRAC 3 cut(s) 199, 1909, 2086
HinfI GANTC 7 cut(s) 275, 577, 701, 896, 1259, 1373, 1993
HpaII CCGG 4 cut(s) 161, 447, 1296, 2059
HphI GGTGA 5 cut(s) 404, 878, 1001, 1452, 1889
Hpy166II GTNNAC 4 cut(s) 199, 1880, 1909, 2086
Hpy188I TCNGA 5 cut(s) 553, 740, 1293, 1372, 2026
Hpy188III TCNNGA 9 cut(s) 337, 705, 781, 900, 1118, 1580, 1901, 1997, 2078
Hpy8I GTNNAC 4 cut(s) 199, 1880, 1909, 2086
Hpy99I CGWCG 1 cut(s) 331
HpyCH4III ACNGT 3 cut(s) 977, 1039, 1213
HpyCH4IV ACGT 4 cut(s) 177, 195, 326, 765
HpyF3I CTNAG 3 cut(s) 552, 1185, 1572
HpySE526I ACGT 4 cut(s) 177, 195, 326, 765
Hsp92I GRCGYC 2 cut(s) 246, 1080
HspAI GCGC 6 cut(s) 191, 246, 267, 292, 298, 1080
KasI GGCGCC 2 cut(s) 245, 1079
KspI CCGCGG 1 cut(s) 120
Kzo9I GATC 6 cut(s) 535, 589, 1222, 1305, 1312, 1897
LmnI GCTCC 4 cut(s) 144, 425, 619, 983
Lsp1109I GCAGC 5 cut(s) 159, 492, 634, 708, 730
LweI GCATC 7 cut(s) 118, 312, 433, 718, 774, 1171, 1711
MabI ACCWGGT 1 cut(s) 1843
MaeI CTAG 4 cut(s) 1044, 1137, 1617, 1901
MaeII ACGT 4 cut(s) 177, 195, 326, 765
MalI GATC 6 cut(s) 537, 591, 1224, 1307, 1314, 1899
MbiI CCGCTC 1 cut(s) 403
MboI GATC 6 cut(s) 535, 589, 1222, 1305, 1312, 1897
MboII GAAGA 9 cut(s) 48, 509, 512, 837, 929, 1526, 1538, 2012, 2080
MfeI CAATTG 1 cut(s) 1509
MflI RGATCY 2 cut(s) 1222, 1897
MhlI GDGCHC 2 cut(s) 1452, 2034
MlsI TGGCCA 1 cut(s) 1032
MluCI AATT 4 cut(s) 912, 1419, 1509, 1727
MluI ACGCGT 2 cut(s) 38, 1682
MluNI TGGCCA 1 cut(s) 1032
Mly113I GGCGCC 2 cut(s) 246, 1080
MlyI GAGTC 4 cut(s) 586, 1253, 1382, 1987
MmeI TCCRAC 1 cut(s) 1200
Mox20I TGGCCA 1 cut(s) 1032
Mph1103I ATGCAT 3 cut(s) 1130, 1704, 1804
MroXI GAANNNNTTC 1 cut(s) 1728
MscI TGGCCA 1 cut(s) 1032
MseI TTAA 7 cut(s) 494, 1103, 1154, 1350, 1418, 1715, 1805
MslI CAYNNNNRTG 1 cut(s) 218
Msp20I TGGCCA 1 cut(s) 1032
MspA1I CMGCKG 4 cut(s) 119, 625, 721, 1325
MspCI CTTAAG 1 cut(s) 1102
MspI CCGG 4 cut(s) 161, 447, 1296, 2059
MspR9I CCNGG 7 cut(s) 161, 372, 448, 1362, 1599, 1642, 1845
MunI CAATTG 1 cut(s) 1509
Mva1269I GAATGC 1 cut(s) 1128
MvaI CCWGG 5 cut(s) 372, 1362, 1599, 1642, 1845
MvnI CGCG 7 cut(s) 40, 119, 267, 294, 300, 861, 1684
NarI GGCGCC 2 cut(s) 246, 1080
NciI CCSGG 2 cut(s) 161, 448
NcoI CCATGG 1 cut(s) 270
NdeI CATATG 2 cut(s) 1720, 1858
NdeII GATC 6 cut(s) 535, 589, 1222, 1305, 1312, 1897
NlaIV GGNNCC 6 cut(s) 9, 247, 774, 1081, 1224, 1590
NmeAIII GCCGAG 2 cut(s) 86, 650
NmuCI GTSAC 4 cut(s) 989, 1039, 1458, 1837
NsiI ATGCAT 3 cut(s) 1130, 1704, 1804
NspI RCATGY 6 cut(s) 380, 715, 746, 1128, 1177, 1635
NspV TTCGAA 1 cut(s) 1380
PaeR7I CTCGAG 2 cut(s) 287, 1502
PceI AGGCCT 1 cut(s) 1055
PciI ACATGT 4 cut(s) 711, 742, 1173, 1631
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PctI GAATGC 1 cut(s) 1128
PdmI GAANNNNTTC 1 cut(s) 1728
PfeI GAWTC 3 cut(s) 275, 701, 896
PflMI CCANNNNNTGG 3 cut(s) 787, 1028, 1271
PfoI TCCNGGA 1 cut(s) 1597
PkrI GCNGC 9 cut(s) 112, 121, 149, 245, 302, 507, 624, 720, 723
PleI GAGTC 4 cut(s) 585, 1253, 1381, 1987
PluTI GGCGCC 2 cut(s) 249, 1083
PpsI GAGTC 4 cut(s) 585, 1253, 1381, 1987
PpuMI RGGWCCY 2 cut(s) 1589, 1736
PscI ACATGT 4 cut(s) 711, 742, 1173, 1631
PshAI GACNNNNGTC 1 cut(s) 1587
PshBI ATTAAT 3 cut(s) 1350, 1418, 1805
Psp124BI GAGCTC 2 cut(s) 1452, 2034
Psp1406I AACGTT 1 cut(s) 765
Psp5II RGGWCCY 2 cut(s) 1589, 1736
Psp6I CCWGG 5 cut(s) 370, 1360, 1597, 1640, 1843
PspFI CCCAGC 1 cut(s) 1007
PspGI CCWGG 5 cut(s) 370, 1360, 1597, 1640, 1843
PspN4I GGNNCC 6 cut(s) 9, 247, 774, 1081, 1224, 1590
PspPI GGNCC 8 cut(s) 8, 30, 163, 223, 455, 666, 1589, 1736
PspPPI RGGWCCY 2 cut(s) 1589, 1736
PspXI VCTCGAGB 2 cut(s) 287, 1502
PstI CTGCAG 1 cut(s) 547
PstNI CAGNNNCTG 2 cut(s) 551, 787
PsuI RGATCY 2 cut(s) 1222, 1897
PvuII CAGCTG 3 cut(s) 625, 721, 1325
RsaI GTAC 1 cut(s) 1786
RsaNI GTAC 1 cut(s) 1785
RseI CAYNNNNRTG 1 cut(s) 218
SacI GAGCTC 2 cut(s) 1452, 2034
SacII CCGCGG 1 cut(s) 120
SaqAI TTAA 7 cut(s) 494, 1103, 1154, 1350, 1418, 1715, 1805
SatI GCNGC 9 cut(s) 111, 120, 148, 244, 301, 506, 623, 719, 722
Sau3AI GATC 6 cut(s) 535, 589, 1222, 1305, 1312, 1897
Sau96I GGNCC 8 cut(s) 8, 30, 163, 223, 455, 666, 1589, 1736
SchI GAGTC 4 cut(s) 586, 1253, 1382, 1987
ScrFI CCNGG 7 cut(s) 161, 372, 448, 1362, 1599, 1642, 1845
SduI GDGCHC 2 cut(s) 1452, 2034
SexAI ACCWGGT 1 cut(s) 1843
SfaNI GCATC 7 cut(s) 118, 312, 433, 718, 774, 1171, 1711
SfcI CTRYAG 3 cut(s) 137, 543, 612
SfiI GGCCNNNNNGGCC 1 cut(s) 119
SfoI GGCGCC 2 cut(s) 247, 1081
Sfr274I CTCGAG 2 cut(s) 287, 1502
Sfr303I CCGCGG 1 cut(s) 120
SfuI TTCGAA 1 cut(s) 1380
SgrBI CCGCGG 1 cut(s) 120
SinI GGWCC 5 cut(s) 30, 223, 455, 1589, 1736
SlaI CTCGAG 2 cut(s) 287, 1502
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 3 cut(s) 287, 1102, 1502
SmoI CTYRAG 3 cut(s) 287, 1102, 1502
Sse9I AATT 4 cut(s) 912, 1419, 1509, 1727
SseBI AGGCCT 1 cut(s) 1055
SspDI GGCGCC 2 cut(s) 245, 1079
SspI AATATT 2 cut(s) 828, 1939
SspMI CTAG 4 cut(s) 1044, 1137, 1617, 1901
SstI GAGCTC 2 cut(s) 1452, 2034
StuI AGGCCT 1 cut(s) 1055
StyD4I CCNGG 7 cut(s) 159, 370, 446, 1360, 1597, 1640, 1843
StyI CCWWGG 3 cut(s) 270, 318, 817
TaaI ACNGT 3 cut(s) 977, 1039, 1213
TaiI ACGT 4 cut(s) 180, 198, 329, 768
TaqI TCGA 7 cut(s) 156, 288, 420, 1380, 1503, 1732, 1886
TasI AATT 4 cut(s) 912, 1419, 1509, 1727
TauI GCSGC 4 cut(s) 113, 122, 246, 303
TfiI GAWTC 3 cut(s) 275, 701, 896
Tru1I TTAA 7 cut(s) 494, 1103, 1154, 1350, 1418, 1715, 1805
Tru9I TTAA 7 cut(s) 494, 1103, 1154, 1350, 1418, 1715, 1805
TscAI CASTG 3 cut(s) 245, 982, 1681
TseFI GTSAC 4 cut(s) 989, 1039, 1458, 1837
TseI GCWGC 5 cut(s) 147, 505, 622, 718, 721
Tsp45I GTSAC 4 cut(s) 989, 1039, 1458, 1837
TspDTI ATGAA 6 cut(s) 582, 866, 956, 1539, 1737, 1974
TspGWI ACGGA 2 cut(s) 1910, 2080
TspRI CASTG 3 cut(s) 245, 982, 1681
Van91I CCANNNNNTGG 3 cut(s) 787, 1028, 1271
Vha464I CTTAAG 1 cut(s) 1102
VpaK11BI GGWCC 5 cut(s) 30, 223, 455, 1589, 1736
VspI ATTAAT 3 cut(s) 1350, 1418, 1805
XagI CCTNNNNNAGG 1 cut(s) 1596
XapI RAATTY 1 cut(s) 1727
XbaI TCTAGA 1 cut(s) 1900
XceI RCATGY 6 cut(s) 380, 715, 746, 1128, 1177, 1635
XhoI CTCGAG 2 cut(s) 287, 1502
XmnI GAANNNNTTC 1 cut(s) 1728
XspI CTAG 4 cut(s) 1044, 1137, 1617, 1901
Zsp2I ATGCAT 3 cut(s) 1130, 1704, 1804
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.