Rh2DG357600

GTP-binding protein TypA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0005874
Physical Location & Seq
Reverse (-)
50330130 .. 50352576
22447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG357600.1

Sequence Viewer

Length: 1398 bp
ATGGATAATATCTTCCAGGAGCAGCTGGATTTTCCAGTGCTATATGCTTCTGCTAAAGAAGGATGGGCCTCGGCTACTTTCACTAAAGAACCTCCTGCTGATTCAAGAAACATGTCGCAGCTGCTTGATGCTATTATCAGACATGTTCCCCCACCAAGTGCAAACGTTGAGGCACCTTTCCAGATGCTGGTTTCCATGATGGAAAAGGACTTCTACCTTGGGCGAATATTGACTGGACGGGTTTCTTCTGGCTGTGTTCGCGTTGGTGATAGAGTTCATGGGCTTCGTAACAAAGATTCTGGAGTTGAGAAAATTGAAGAAGGGAAGGTTGTGAAACTGATGAAAAAGAAGGGAACAAATATGGTTCTCATTGACAGTGCTGGAGCGGGTGACATAATATCAATGGCTGGGTTAGCAAGTCCATCTATTGGCCATACAGTCACTAGCGTTGAGGTAATGACTGCATTACCAACCGTTGAGTTGGATCCACCAACGATTTCCATGACTTTTGGTGTAAATGACTCTCCATTAGCTGGCCGTGATGGTAGCCATCTGACCGGAGGAAAGATCGGGGATCGGCTATCAGCTGAAGCAGAAACAAATCTTGCTATTAATGTCCTCCCAGGCTTATCAGAATCATTCGAAGTTCAGGGGAGGGGAGAACTGCAACTCGGTATATTAATTGAGAATATGAGGCGTGAAGGTTTTGAGCTCTCTGTGTCACCACCCAAAGTCATGTATAAAACAGAGAATGGTCAAAGGCTCGAGCCAATTGAAGAAGTTACTATAGAGGTAAATGAAGACCATGTGGGGTTAGTTATGGAAGCCTTATCTCATAGGCGAGCTGAGGTTCTTGACATGGGTCCTGTTCCAGGAAATGATGGCAGGACTAGATTGTCTTTGACATGTCCATCCAGGGGACTGGTTGGTTACAGAAGTGTATTTAGCAGTGCCACGCGTGGAACTGGGTTTATGCATCGTGCTTTCTTAACATATGAAAAATTTCGAGGTCCTCTTGGAAATGTGCGGAAAGGGGTTTTGGTGTCAATGGGCTATGGTACAATCACATCCTATGCATTAATGAGTTTAGAACCTCGTGGAACTCTATTTGTGACACCTGGTATGGAGACATATGATGGCATGATTGTTGGTGAACATTCGAGGGAGACGGATCTAGATGTCAACCCAGTAAGGAACAAAGAACTTACAAATATTCGTTCTGCTGGCAAGGATGAAAATGTGAAACTATCTCCTCCTCGCCTTATGACTCTTGAAGAAGCCATAGGGTATGTAGCCTCGGATGAGCTCATTGAGGTTACACCGAAGTCCATCCGGTTACGGAAGAAATATCTGGATGTCAACAAGCGTAAAACCATGAGCAAAAGGCCAAAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFG_C GTP_EFTU GTP_EFTU_D2

Protein Analysis

465

Amino Acids

50.9

Weight (kDa)

6.32

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-G_D2 PF22042 59 - 149 7.7e-08 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 74 - 148 9.9e-09 Elongation factor Tu domain 2
EFG_C PF00679 255 - 337 5.9e-20 Elongation factor G C-terminus
BipA_C PF21018 344 - 452 1.9e-45 TypA/BipA C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 895
AccB1I GGYRCC 1 cut(s) 172
AccB7I CCANNNNNTGG 3 cut(s) 187, 428, 533
AccBSI CCGCTC 1 cut(s) 386
AccII CGCG 2 cut(s) 261, 958
AciI CCGC 2 cut(s) 386, 1027
AclI AACGTT 1 cut(s) 165
AclWI GGATC 4 cut(s) 479, 492, 582, 1179
AcoI YGGCCR 2 cut(s) 430, 535
AcsI RAATTY 1 cut(s) 1001
AcuI CTGAAG 1 cut(s) 609
AdeI CACNNNGTG 1 cut(s) 158
AfaI GTAC 1 cut(s) 1060
AfiI CCNNNNNNNGG 5 cut(s) 187, 428, 533, 872, 917
AflIII ACRYGT 4 cut(s) 111, 142, 905, 956
AgsI TTSAA 4 cut(s) 105, 317, 776, 1274
AjnI CCWGG 5 cut(s) 15, 622, 871, 914, 1117
AjuI GAANNNNNNNTTGG 2 cut(s) 1022, 1054
AluBI AGCT 7 cut(s) 25, 121, 533, 587, 712, 845, 1306
AluI AGCT 7 cut(s) 25, 121, 533, 587, 712, 845, 1306
Alw21I GWGCWC 2 cut(s) 714, 1308
Alw26I GTCTC 2 cut(s) 1121, 1160
AlwI GGATC 4 cut(s) 479, 492, 582, 1179
AlwNI CAGNNNCTG 1 cut(s) 187
Ama87I CYCGRG 1 cut(s) 764
AoxI GGCC 4 cut(s) 66, 430, 535, 1385
ApeKI GCWGC 3 cut(s) 22, 118, 121
ApoI RAATTY 1 cut(s) 1001
AseI ATTAAT 3 cut(s) 612, 680, 1079
Asp700I GAANNNNTTC 1 cut(s) 1002
AspS9I GGNCC 3 cut(s) 66, 863, 1010
AsuHPI GGTGA 4 cut(s) 278, 401, 714, 1163
AsuII TTCGAA 1 cut(s) 642
AvaI CYCGRG 1 cut(s) 764
AvaII GGWCC 2 cut(s) 863, 1010
BalI TGGCCA 1 cut(s) 432
BamHI GGATCC 1 cut(s) 484
BanI GGYRCC 1 cut(s) 172
BanII GRGCYC 2 cut(s) 714, 1308
BauI CACGAG 1 cut(s) 1095
BbsI GAAGAC 1 cut(s) 807
Bbv12I GWGCWC 2 cut(s) 714, 1308
BbvCI CCTCAGC 1 cut(s) 846
BbvI GCAGC 3 cut(s) 34, 108, 130
BccI CCATC 9 cut(s) 57, 193, 430, 536, 558, 875, 919, 1130, 1337
BceAI ACGGC 1 cut(s) 522
BciT130I CCWGG 5 cut(s) 17, 624, 873, 916, 1119
BcoDI GTCTC 2 cut(s) 1121, 1160
BfaI CTAG 3 cut(s) 444, 891, 1175
BfmI CTRYAG 1 cut(s) 786
BisI GCNGC 3 cut(s) 23, 119, 122
BlsI GCNGC 3 cut(s) 24, 120, 123
Bme1390I CCNGG 5 cut(s) 17, 624, 873, 916, 1119
Bme18I GGWCC 2 cut(s) 863, 1010
BmeT110I CYCGRG 1 cut(s) 764
BmgT120I GGNCC 3 cut(s) 66, 863, 1010
BmiI GGNNCC 3 cut(s) 174, 486, 864
BmrFI CCNGG 5 cut(s) 17, 624, 873, 916, 1119
BmrI ACTGGG 2 cut(s) 975, 1181
BmsI GCATC 3 cut(s) 118, 174, 985
BmuI ACTGGG 2 cut(s) 975, 1181
BoxI GACNNNNGTC 1 cut(s) 861
BpiI GAAGAC 1 cut(s) 807
BpmI CTGGAG 2 cut(s) 321, 402
Bpu10I CCTNAGC 1 cut(s) 846
Bpu14I TTCGAA 1 cut(s) 642
BsaJI CCNNGG 5 cut(s) 69, 217, 622, 915, 1296
BsaWI WCCGGW 2 cut(s) 557, 1332
Bsc4I CCNNNNNNNGG 5 cut(s) 187, 428, 533, 872, 917
Bse1I ACTGG 5 cut(s) 35, 238, 927, 970, 1187
BseBI CCWGG 5 cut(s) 17, 624, 873, 916, 1119
BseDI CCNNGG 5 cut(s) 69, 217, 622, 915, 1296
BseGI GGATG 7 cut(s) 68, 911, 1067, 1237, 1306, 1329, 1360
BseLI CCNNNNNNNGG 5 cut(s) 187, 428, 533, 872, 917
BseMII CTCAG 1 cut(s) 837
BseNI ACTGG 5 cut(s) 35, 238, 927, 970, 1187
BseRI GAGGAG 2 cut(s) 1242, 1245
BseXI GCAGC 3 cut(s) 34, 108, 130
BseYI CCCAGC 1 cut(s) 407
Bsh1236I CGCG 2 cut(s) 261, 958
BshFI GGCC 4 cut(s) 68, 432, 537, 1387
BshNI GGYRCC 1 cut(s) 172
BsiHKAI GWGCWC 2 cut(s) 714, 1308
BsiHKCI CYCGRG 1 cut(s) 764
BsiSI CCGG 2 cut(s) 558, 1333
BslFI GGGAC 1 cut(s) 933
BslI CCNNNNNNNGG 5 cut(s) 187, 428, 533, 872, 917
BsmAI GTCTC 2 cut(s) 1121, 1160
BsmBI CGTCTC 1 cut(s) 1160
BsmFI GGGAC 1 cut(s) 933
BsnI GGCC 4 cut(s) 68, 432, 537, 1387
BsoBI CYCGRG 1 cut(s) 764
Bsp119I TTCGAA 1 cut(s) 642
Bsp1286I GDGCHC 2 cut(s) 714, 1308
Bsp143I GATC 4 cut(s) 484, 567, 574, 1171
BspACI CCGC 2 cut(s) 386, 1027
BspANI GGCC 4 cut(s) 68, 432, 537, 1387
BspCNI CTCAG 1 cut(s) 838
BspFNI CGCG 2 cut(s) 261, 958
BspLI GGNNCC 3 cut(s) 174, 486, 864
BspPI GGATC 4 cut(s) 479, 492, 582, 1179
BspT104I TTCGAA 1 cut(s) 642
BspT107I GGYRCC 1 cut(s) 172
BsrBI CCGCTC 1 cut(s) 386
BsrI ACTGG 5 cut(s) 35, 238, 927, 970, 1187
BssECI CCNNGG 5 cut(s) 69, 217, 622, 915, 1296
BssMI GATC 4 cut(s) 484, 567, 574, 1171
BssSI CACGAG 1 cut(s) 1095
BssT1I CCWWGG 1 cut(s) 217
Bst2BI CACGAG 1 cut(s) 1095
Bst2UI CCWGG 5 cut(s) 17, 624, 873, 916, 1119
Bst4CI ACNGT 3 cut(s) 377, 439, 475
BstBI TTCGAA 1 cut(s) 642
BstC8I GCNNGC 3 cut(s) 535, 843, 1225
BstDEI CTNAG 1 cut(s) 846
BstENI CCTNNNNNAGG 1 cut(s) 870
BstF5I GGATG 7 cut(s) 68, 911, 1067, 1237, 1306, 1329, 1360
BstFNI CGCG 2 cut(s) 261, 958
BstKTI GATC 4 cut(s) 487, 570, 577, 1174
BstMAI GTCTC 2 cut(s) 1121, 1160
BstMBI GATC 4 cut(s) 484, 567, 574, 1171
BstMWI GCNNNNNNNGC 2 cut(s) 258, 413
BstNI CCWGG 5 cut(s) 17, 624, 873, 916, 1119
BstNSI RCATGY 3 cut(s) 115, 146, 909
BstPAI GACNNNNGTC 1 cut(s) 861
BstSCI CCNGG 5 cut(s) 15, 622, 871, 914, 1117
BstSFI CTRYAG 1 cut(s) 786
BstUI CGCG 2 cut(s) 261, 958
BstV1I GCAGC 3 cut(s) 34, 108, 130
BstV2I GAAGAC 1 cut(s) 807
BstX2I RGATCY 2 cut(s) 484, 1171
BstXI CCANNNNNNTGG 1 cut(s) 922
BstYI RGATCY 2 cut(s) 484, 1171
BsuRI GGCC 4 cut(s) 68, 432, 537, 1387
BtsCI GGATG 7 cut(s) 68, 911, 1067, 1237, 1306, 1329, 1360
BtsI GCAGTG 1 cut(s) 955
BtsIMutI CAGTG 3 cut(s) 42, 382, 955
Cac8I GCNNGC 3 cut(s) 535, 843, 1225
CaiI CAGNNNCTG 1 cut(s) 187
Cfr13I GGNCC 3 cut(s) 66, 863, 1010
CsiI ACCWGGT 1 cut(s) 1117
Csp6I GTAC 1 cut(s) 1059
CviQI GTAC 1 cut(s) 1059
DdeI CTNAG 1 cut(s) 846
DpnI GATC 4 cut(s) 486, 569, 576, 1173
DpnII GATC 4 cut(s) 484, 567, 574, 1171
DraIII CACNNNGTG 1 cut(s) 158
DrdI GACNNNNNNGTC 1 cut(s) 895
DseDI GACNNNNNNGTC 1 cut(s) 895
EaeI YGGCCR 2 cut(s) 430, 535
Ecl136II GAGCTC 2 cut(s) 712, 1306
Eco130I CCWWGG 1 cut(s) 217
Eco24I GRGCYC 2 cut(s) 714, 1308
Eco47I GGWCC 2 cut(s) 863, 1010
Eco53kI GAGCTC 2 cut(s) 712, 1306
Eco57I CTGAAG 1 cut(s) 609
Eco88I CYCGRG 1 cut(s) 764
EcoICRI GAGCTC 2 cut(s) 712, 1306
EcoNI CCTNNNNNAGG 1 cut(s) 870
EcoO109I RGGNCCY 2 cut(s) 863, 1010
EcoRII CCWGG 5 cut(s) 15, 622, 871, 914, 1117
EcoT14I CCWWGG 1 cut(s) 217
EcoT22I ATGCAT 2 cut(s) 978, 1078
EcoT38I GRGCYC 2 cut(s) 714, 1308
ErhI CCWWGG 1 cut(s) 217
Esp3I CGTCTC 1 cut(s) 1160
FaqI GGGAC 1 cut(s) 933
FauI CCCGC 1 cut(s) 379
FauNDI CATATG 2 cut(s) 994, 1132
Fnu4HI GCNGC 3 cut(s) 23, 119, 122
FokI GGATG 7 cut(s) 75, 898, 1054, 1244, 1313, 1316, 1367
FriOI GRGCYC 2 cut(s) 714, 1308
Fsp4HI GCNGC 3 cut(s) 23, 119, 122
FspBI CTAG 3 cut(s) 444, 891, 1175
GluI GCNGC 3 cut(s) 23, 119, 122
GsaI CCCAGC 1 cut(s) 411
GsuI CTGGAG 2 cut(s) 321, 402
HaeIII GGCC 4 cut(s) 68, 432, 537, 1387
HapII CCGG 2 cut(s) 558, 1333
HincII GTYRAC 2 cut(s) 1183, 1360
HindII GTYRAC 2 cut(s) 1183, 1360
HinfI GANTC 5 cut(s) 101, 296, 521, 635, 1267
HpaII CCGG 2 cut(s) 558, 1333
HphI GGTGA 4 cut(s) 278, 401, 714, 1163
Hpy166II GTNNAC 3 cut(s) 1154, 1183, 1360
Hpy188I TCNGA 4 cut(s) 140, 555, 634, 1300
Hpy188III TCNNGA 7 cut(s) 105, 181, 300, 854, 1175, 1271, 1352
Hpy8I GTNNAC 3 cut(s) 1154, 1183, 1360
HpyAV CCTTC 5 cut(s) 53, 314, 319, 343, 695
HpyCH4III ACNGT 3 cut(s) 377, 439, 475
HpyCH4IV ACGT 1 cut(s) 165
HpyCH4V TGCA 5 cut(s) 161, 464, 667, 976, 1076
HpyF10VI GCNNNNNNNGC 2 cut(s) 258, 413
HpyF3I CTNAG 1 cut(s) 846
HpySE526I ACGT 1 cut(s) 165
Kzo9I GATC 4 cut(s) 484, 567, 574, 1171
LmnI GCTCC 2 cut(s) 19, 383
Lsp1109I GCAGC 3 cut(s) 34, 108, 130
LweI GCATC 3 cut(s) 118, 174, 985
MabI ACCWGGT 1 cut(s) 1117
MaeI CTAG 3 cut(s) 444, 891, 1175
MaeII ACGT 1 cut(s) 165
MaeIII GTNAC 9 cut(s) 287, 389, 439, 720, 781, 929, 1111, 1315, 1335
MalI GATC 4 cut(s) 486, 569, 576, 1173
MbiI CCGCTC 1 cut(s) 386
MboI GATC 4 cut(s) 484, 567, 574, 1171
MboII GAAGA 7 cut(s) 4, 237, 329, 788, 812, 1286, 1354
MfeI CAATTG 1 cut(s) 771
MflI RGATCY 2 cut(s) 484, 1171
MhlI GDGCHC 2 cut(s) 714, 1308
MlsI TGGCCA 1 cut(s) 432
MluCI AATT 4 cut(s) 312, 681, 771, 1001
MluI ACGCGT 1 cut(s) 956
MluNI TGGCCA 1 cut(s) 432
MlyI GAGTC 2 cut(s) 515, 1261
MmeI TCCRAC 1 cut(s) 462
Mox20I TGGCCA 1 cut(s) 432
Mph1103I ATGCAT 2 cut(s) 978, 1078
MroXI GAANNNNTTC 1 cut(s) 1002
MscI TGGCCA 1 cut(s) 432
MseI TTAA 4 cut(s) 612, 680, 989, 1079
Msp20I TGGCCA 1 cut(s) 432
MspA1I CMGCKG 3 cut(s) 25, 121, 587
MspI CCGG 2 cut(s) 558, 1333
MspR9I CCNGG 5 cut(s) 17, 624, 873, 916, 1119
MunI CAATTG 1 cut(s) 771
MvaI CCWGG 5 cut(s) 17, 624, 873, 916, 1119
MvnI CGCG 2 cut(s) 261, 958
MwoI GCNNNNNNNGC 2 cut(s) 258, 413
NdeI CATATG 2 cut(s) 994, 1132
NdeII GATC 4 cut(s) 484, 567, 574, 1171
NlaIV GGNNCC 3 cut(s) 174, 486, 864
NmeAIII GCCGAG 1 cut(s) 50
NmuCI GTSAC 4 cut(s) 389, 439, 720, 1111
NsiI ATGCAT 2 cut(s) 978, 1078
NspI RCATGY 3 cut(s) 115, 146, 909
NspV TTCGAA 1 cut(s) 642
PaeR7I CTCGAG 1 cut(s) 764
PciI ACATGT 3 cut(s) 111, 142, 905
PdmI GAANNNNTTC 1 cut(s) 1002
PfeI GAWTC 3 cut(s) 101, 296, 635
PflMI CCANNNNNTGG 3 cut(s) 187, 428, 533
PfoI TCCNGGA 2 cut(s) 15, 871
PkrI GCNGC 3 cut(s) 24, 120, 123
PleI GAGTC 2 cut(s) 515, 1261
PpsI GAGTC 2 cut(s) 515, 1261
PpuMI RGGWCCY 2 cut(s) 863, 1010
PscI ACATGT 3 cut(s) 111, 142, 905
PshAI GACNNNNGTC 1 cut(s) 861
PshBI ATTAAT 3 cut(s) 612, 680, 1079
Psp124BI GAGCTC 2 cut(s) 714, 1308
Psp1406I AACGTT 1 cut(s) 165
Psp5II RGGWCCY 2 cut(s) 863, 1010
Psp6I CCWGG 5 cut(s) 15, 622, 871, 914, 1117
PspFI CCCAGC 1 cut(s) 407
PspGI CCWGG 5 cut(s) 15, 622, 871, 914, 1117
PspN4I GGNNCC 3 cut(s) 174, 486, 864
PspPI GGNCC 3 cut(s) 66, 863, 1010
PspPPI RGGWCCY 2 cut(s) 863, 1010
PspXI VCTCGAGB 1 cut(s) 764
PstNI CAGNNNCTG 1 cut(s) 187
PsuI RGATCY 2 cut(s) 484, 1171
PvuII CAGCTG 3 cut(s) 25, 121, 587
RsaI GTAC 1 cut(s) 1060
RsaNI GTAC 1 cut(s) 1059
SacI GAGCTC 2 cut(s) 714, 1308
SaqAI TTAA 4 cut(s) 612, 680, 989, 1079
SatI GCNGC 3 cut(s) 23, 119, 122
Sau3AI GATC 4 cut(s) 484, 567, 574, 1171
Sau96I GGNCC 3 cut(s) 66, 863, 1010
SchI GAGTC 2 cut(s) 515, 1261
ScrFI CCNGG 5 cut(s) 17, 624, 873, 916, 1119
SduI GDGCHC 2 cut(s) 714, 1308
SexAI ACCWGGT 1 cut(s) 1117
SfaNI GCATC 3 cut(s) 118, 174, 985
SfcI CTRYAG 1 cut(s) 786
Sfr274I CTCGAG 1 cut(s) 764
SfuI TTCGAA 1 cut(s) 642
SinI GGWCC 2 cut(s) 863, 1010
SlaI CTCGAG 1 cut(s) 764
SmlI CTYRAG 1 cut(s) 764
SmoI CTYRAG 1 cut(s) 764
Sse9I AATT 4 cut(s) 312, 681, 771, 1001
SsiI CCGC 2 cut(s) 386, 1027
SspI AATATT 2 cut(s) 228, 1213
SspMI CTAG 3 cut(s) 444, 891, 1175
SstI GAGCTC 2 cut(s) 714, 1308
StyD4I CCNGG 5 cut(s) 15, 622, 871, 914, 1117
StyI CCWWGG 1 cut(s) 217
TaaI ACNGT 3 cut(s) 377, 439, 475
TaiI ACGT 1 cut(s) 168
TaqI TCGA 4 cut(s) 642, 765, 1006, 1160
TasI AATT 4 cut(s) 312, 681, 771, 1001
TfiI GAWTC 3 cut(s) 101, 296, 635
Tru1I TTAA 4 cut(s) 612, 680, 989, 1079
Tru9I TTAA 4 cut(s) 612, 680, 989, 1079
TscAI CASTG 3 cut(s) 42, 382, 955
TseFI GTSAC 4 cut(s) 389, 439, 720, 1111
TseI GCWGC 3 cut(s) 22, 118, 121
Tsp45I GTSAC 4 cut(s) 389, 439, 720, 1111
TspDTI ATGAA 5 cut(s) 266, 356, 813, 1011, 1248
TspGWI ACGGA 2 cut(s) 1184, 1354
TspRI CASTG 3 cut(s) 42, 382, 955
Van91I CCANNNNNTGG 3 cut(s) 187, 428, 533
VpaK11BI GGWCC 2 cut(s) 863, 1010
VspI ATTAAT 3 cut(s) 612, 680, 1079
XagI CCTNNNNNAGG 1 cut(s) 870
XapI RAATTY 1 cut(s) 1001
XbaI TCTAGA 1 cut(s) 1174
XceI RCATGY 3 cut(s) 115, 146, 909
XhoI CTCGAG 1 cut(s) 764
XmnI GAANNNNTTC 1 cut(s) 1002
XspI CTAG 3 cut(s) 444, 891, 1175
Zsp2I ATGCAT 2 cut(s) 978, 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.