MD09G1241200.v1.1

GTP-binding protein TypA

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005874
Physical Location & Seq
Forward (+)
30841538 .. 30851939
10402 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1241200.v1.1.491

Sequence Viewer

Length: 2019 bp
ATGGCGGGGCCGTTGCTTCGCTCTCTCTGGTCCTCCACGCGCAACTCTTTCTCTTCCTCCTCTGCGTCCCCCACTCTCAAACCCTACCCATTAACCCCACGTGCATCTCCACTCGCACGCGCCTTCTCGGCAGCCACTGCGGCCTCCAATGCTAGTACTTCCCCGAACACCGTCCTGGATCCGAGCCGCCTCCGCAACGTGGCAGTAATAGCCCATGTGGACCACGGCAAGACGACGCTCATGGATCGCCTGCTCCGCCAGTGCGGCGCCGACATCCCCCACGAACGAGCCATGGACTCCATCAGCCTCGAGCGCGAGCGCGGAATTACCATCGCCTCCAAGGTCACGTCAATTCCATGGAAGGAGATTGAGTTGAACATGGTTGATACGCCTGGACATGCCGATTTCGGTGGTGAAGTTGAGCGGGTTGTTGGTATGGTTGAGGGTGCAATTTTAGTTGTTGATGCTGGGGAAGGTCCGCTTGCACAGACCAAGTTCGTACTTGCTAAAGCCTTAAAATATGGGCTGCGCCCCATTCTTCTTCTAAACAAAGTAGATCGTCCTGCAGTTTCTGAGGAGATGTGTAATGAAGTTGAGAGTTTGGTGTTTGATCTTTTTGCAAATCTTGGTGCTACAGAGGAGCAGCTAGATTTTCCTGTGCTGTATGCTTCTGCTAAAGAAGGATGGGCTTCCACCACTTTCACCAAAGATCCTCCTGCTGATGCAAGAAATATGTCACGACTGCTTGATGCCATTATAAGTCATGTTCCTCCACCAACAGCCAATCTTGAGGCACCTTTCCAAATGCTGGTTTCTATGATGGAACGAGACTTCTACCTTGGACGAATATTGACCGGACGAGTTTCTTCTGGCAGCATTCGCATTGGTGACAGAGTTCATGGGCTTCGTCACAAAGATTCTGGAGTTGAGAAAATTGAAGAAGGGAAGGTTGTGAAGCTGATGAAAAAGAAGGGAACACATATGGTCGTAATTGATTGTGCTGGAGCTGGTGACATAGTGTCAATGGCTGGGTTAGCAAGCCCATCTATTGGCCATACAGTTGCAAGCGTTGAGATTATGACTGCATTGCCCACCGTTGAGTTGGATCCCCCAACTATTTCCATGACTTTTGGTGTCAATGACTCTCCCTTAGCTGGTCGTGATGGTACCCATCTCACTGGAGGAAAAATTGGCGATCGACTATCAGCTGAATCGGAAACAAATCTTGCCATTAATGTTCTCCCAGGCTTATCAGAATCATTTGAAGTTCAGGGGAGGGGAGAACTTCAACTGGGTATTTTAATTGAGAATATGAGGCGGGAAGGCTTCGAACTGTCTGTCTCACCACCCAAAGTCATGTACAAAACAGAGAATGGTCAAAAACTTGAGCCAATTGAAGAAGTTACTATTGAGGTAAATGAAGAGCATGTGGGGTTAGTAATGCAAGCCTTATCTAATAGGCGTGCTGAGGTTATCGATATGGGTCCTGTTCCAGGAAGCGCTGACCGGACTAGATTGTCTTTGACATGTCCATCAAGGGGCCTGGTTGGTTACAGAAGCGTATTTAGCAGTGACACACGTGGAACTGGATTTATGCATCGTGCTTTCTTAAAATATGAGAAATATCGAGGTCCTCTTGGAGATGTCAGGAAAGGAGTGTTGGTGTCAATGGGCTATGGTACAATCACAGCGTATGCCCTAGTGAGTTTAGAACCTCGCGGAACTCTTTTTGTCACTCCTGGGATGGAGACATATGATGGCATGATTGTTGGCGAGCACTCGAGGGACACAGATCTAGATGTCAACCCAGTTAGAAATAAGGAACTTACAAACATACGTGCTGCTAGCAAGGATGAGAATGTGAAACTATCTCCTCCCCGCCTTATGACTCTTGAAGAAGCAATAGGGTATGTAGCCTCTGATGAGCTGATTGAGGTTACACCAAAGACAATACGGTTAAGGAAGAAATATCTGGACGTTAACAAGCGTAAAAGCATGAGTAAAAGGCAAAAGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EFG_C GTP_EFTU GTP_EFTU_D2

Protein Analysis

673

Amino Acids

73.07

Weight (kDa)

6.41

Isoelectric Point (pI)

39.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 63 - 258 6.7e-53 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 266 - 356 2.8e-07 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 281 - 354 4.4e-08 Elongation factor Tu domain 2
EFG_C PF00679 461 - 545 6.3e-20 Elongation factor G C-terminus
BipA_C PF21018 551 - 659 1.4e-44 TypA/BipA C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 758
AasI GACNNNNNNGTC 1 cut(s) 1516
Acc65I GGTACC 1 cut(s) 1166
AccB1I GGYRCC 3 cut(s) 266, 793, 1166
AccB7I CCANNNNNTGG 2 cut(s) 808, 1049
AccBSI CCGCTC 1 cut(s) 424
AccII CGCG 5 cut(s) 40, 120, 315, 321, 1719
AclWI GGATC 6 cut(s) 173, 186, 252, 704, 1100, 1113
AcoI YGGCCR 1 cut(s) 1051
AcvI CACGTG 2 cut(s) 101, 1580
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 5 cut(s) 157, 501, 1168, 1361, 1681
AfeI AGCGCT 1 cut(s) 1501
AfiI CCNNNNNNNGG 6 cut(s) 199, 808, 1049, 1154, 1493, 1538
AflIII ACRYGT 2 cut(s) 1526, 1577
AgsI TTSAA 6 cut(s) 376, 938, 1265, 1289, 1397, 1895
AhdI GACNNNNNGTC 1 cut(s) 1018
AjiI CACGTC 1 cut(s) 348
AjnI CCWGG 6 cut(s) 174, 391, 1243, 1492, 1542, 1738
AjuI GAANNNNNNNTTGG 2 cut(s) 1643, 1675
AluBI AGCT 6 cut(s) 646, 958, 1007, 1154, 1208, 1927
AluI AGCT 6 cut(s) 646, 958, 1007, 1154, 1208, 1927
Alw21I GWGCWC 1 cut(s) 1779
Alw26I GTCTC 3 cut(s) 822, 1345, 1742
AlwI GGATC 6 cut(s) 173, 186, 252, 704, 1100, 1113
AlwNI CAGNNNCTG 2 cut(s) 137, 572
Ama87I CYCGRG 2 cut(s) 308, 1780
Aor51HI AGCGCT 1 cut(s) 1501
AoxI GGCC 4 cut(s) 8, 141, 1051, 1540
ApeKI GCWGC 5 cut(s) 131, 526, 643, 873, 1841
ArsI GACNNNNNNTTYG 2 cut(s) 1323, 1355
AseI ATTAAT 1 cut(s) 1233
Asp718I GGTACC 1 cut(s) 1166
AspLEI GCGC 7 cut(s) 42, 122, 269, 315, 321, 531, 1502
AspS9I GGNCC 7 cut(s) 8, 30, 220, 476, 1484, 1540, 1631
AsuHPI GGTGA 5 cut(s) 425, 694, 899, 1022, 1335
AsuII TTCGAA 1 cut(s) 1329
AsuNHI GCTAGC 1 cut(s) 1844
AvaI CYCGRG 2 cut(s) 308, 1780
AvaII GGWCC 5 cut(s) 30, 220, 476, 1484, 1631
BalI TGGCCA 1 cut(s) 1053
BamHI GGATCC 2 cut(s) 178, 1105
BanI GGYRCC 3 cut(s) 266, 793, 1166
BbrPI CACGTG 2 cut(s) 101, 1580
Bbv12I GWGCWC 1 cut(s) 1779
BbvCI CCTCAGC 1 cut(s) 1467
BbvI GCAGC 5 cut(s) 143, 513, 655, 885, 1828
BceAI ACGGC 1 cut(s) 241
BciT130I CCWGG 6 cut(s) 176, 393, 1245, 1494, 1544, 1740
BcoDI GTCTC 3 cut(s) 822, 1345, 1742
BfaI CTAG 6 cut(s) 153, 647, 1512, 1700, 1796, 1845
BfmI CTRYAG 2 cut(s) 564, 633
BfoI RGCGCY 2 cut(s) 270, 1503
BglI GCCNNNNNGGC 3 cut(s) 128, 140, 264
BglII AGATCT 1 cut(s) 1792
BisI GCNGC 8 cut(s) 132, 141, 187, 265, 527, 644, 874, 1842
BlsI GCNGC 8 cut(s) 133, 142, 188, 266, 528, 645, 875, 1843
BmcAI AGTACT 1 cut(s) 157
Bme1390I CCNGG 6 cut(s) 176, 393, 1245, 1494, 1544, 1740
Bme18I GGWCC 5 cut(s) 30, 220, 476, 1484, 1631
BmeRI GACNNNNNGTC 1 cut(s) 1018
BmeT110I CYCGRG 2 cut(s) 308, 1780
BmgBI CACGTC 1 cut(s) 348
BmgT120I GGNCC 7 cut(s) 8, 30, 220, 476, 1484, 1540, 1631
BmiI GGNNCC 8 cut(s) 9, 180, 268, 795, 1107, 1168, 1485, 1541
BmrFI CCNGG 6 cut(s) 176, 393, 1245, 1494, 1544, 1740
BmrI ACTGGG 2 cut(s) 1301, 1802
BmsI GCATC 5 cut(s) 113, 454, 712, 739, 1606
BmtI GCTAGC 1 cut(s) 1848
BmuI ACTGGG 2 cut(s) 1301, 1802
BpmI CTGGAG 3 cut(s) 942, 1023, 1200
Bpu10I CCTNAGC 2 cut(s) 1150, 1467
Bpu14I TTCGAA 1 cut(s) 1329
BpuEI CTTGAG 2 cut(s) 809, 1406
Bsa29I ATCGAT 1 cut(s) 1476
BsaAI YACGTR 3 cut(s) 101, 1580, 1838
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 7 cut(s) 223, 291, 339, 356, 838, 1243, 1739
BsaWI WCCGGW 2 cut(s) 854, 1506
BsaXI ACNNNNNCTCC 4 cut(s) 356, 386, 1647, 1677
Bsc4I CCNNNNNNNGG 6 cut(s) 199, 808, 1049, 1154, 1493, 1538
Bse1I ACTGG 5 cut(s) 259, 1183, 1296, 1591, 1808
Bse3DI GCAATG 1 cut(s) 1085
BseBI CCWGG 6 cut(s) 176, 393, 1245, 1494, 1544, 1740
BseCI ATCGAT 1 cut(s) 1476
BseDI CCNNGG 7 cut(s) 223, 291, 339, 356, 838, 1243, 1739
BseGI GGATG 4 cut(s) 273, 689, 1749, 1858
BseLI CCNNNNNNNGG 6 cut(s) 199, 808, 1049, 1154, 1493, 1538
BseMI GCAATG 1 cut(s) 1085
BseMII CTCAG 2 cut(s) 564, 1458
BseNI ACTGG 5 cut(s) 259, 1183, 1296, 1591, 1808
BseRI GAGGAG 4 cut(s) 49, 590, 653, 1863
BseXI GCAGC 5 cut(s) 143, 513, 655, 885, 1828
BseYI CCCAGC 2 cut(s) 467, 1028
Bsh1236I CGCG 5 cut(s) 40, 120, 315, 321, 1719
Bsh1285I CGRYCG 1 cut(s) 1198
BshFI GGCC 4 cut(s) 10, 143, 1053, 1542
BshNI GGYRCC 3 cut(s) 266, 793, 1166
BshVI ATCGAT 1 cut(s) 1476
BsiEI CGRYCG 1 cut(s) 1198
BsiHKAI GWGCWC 1 cut(s) 1779
BsiHKCI CYCGRG 2 cut(s) 308, 1780
BsiSI CCGG 2 cut(s) 855, 1507
BslFI GGGAC 2 cut(s) 52, 1799
BslI CCNNNNNNNGG 6 cut(s) 199, 808, 1049, 1154, 1493, 1538
BsmAI GTCTC 3 cut(s) 822, 1345, 1742
BsmFI GGGAC 2 cut(s) 52, 1799
BsmI GAATGC 1 cut(s) 876
BsnI GGCC 4 cut(s) 10, 143, 1053, 1542
BsoBI CYCGRG 2 cut(s) 308, 1780
Bsp119I TTCGAA 1 cut(s) 1329
Bsp1286I GDGCHC 1 cut(s) 1779
Bsp1407I TGTACA 1 cut(s) 1359
Bsp143I GATC 8 cut(s) 178, 244, 556, 610, 709, 1105, 1195, 1792
Bsp19I CCATGG 2 cut(s) 291, 356
BspANI GGCC 4 cut(s) 10, 143, 1053, 1542
BspCNI CTCAG 2 cut(s) 565, 1459
BspDI ATCGAT 1 cut(s) 1476
BspFNI CGCG 5 cut(s) 40, 120, 315, 321, 1719
BspLI GGNNCC 8 cut(s) 9, 180, 268, 795, 1107, 1168, 1485, 1541
BspMAI CTGCAG 1 cut(s) 568
BspOI GCTAGC 1 cut(s) 1848
BspPI GGATC 6 cut(s) 173, 186, 252, 704, 1100, 1113
BspQI GCTCTTC 1 cut(s) 1416
BspT104I TTCGAA 1 cut(s) 1329
BspT107I GGYRCC 3 cut(s) 266, 793, 1166
BsrBI CCGCTC 1 cut(s) 424
BsrDI GCAATG 1 cut(s) 1085
BsrGI TGTACA 1 cut(s) 1359
BsrI ACTGG 5 cut(s) 259, 1183, 1296, 1591, 1808
BssECI CCNNGG 7 cut(s) 223, 291, 339, 356, 838, 1243, 1739
BssMI GATC 8 cut(s) 178, 244, 556, 610, 709, 1105, 1195, 1792
BssNI GRCGYC 1 cut(s) 267
BssT1I CCWWGG 4 cut(s) 291, 339, 356, 838
Bst2UI CCWGG 6 cut(s) 176, 393, 1245, 1494, 1544, 1740
Bst4CI ACNGT 5 cut(s) 172, 1060, 1096, 1335, 1956
Bst6I CTCTTC 2 cut(s) 58, 1416
BstACI GRCGYC 1 cut(s) 267
BstAPI GCANNNNNTGC 1 cut(s) 137
BstAUI TGTACA 1 cut(s) 1359
BstBAI YACGTR 3 cut(s) 101, 1580, 1838
BstBI TTCGAA 1 cut(s) 1329
BstDEI CTNAG 3 cut(s) 573, 1150, 1467
BstDSI CCRYGG 3 cut(s) 223, 291, 356
BstENI CCTNNNNNAGG 1 cut(s) 1491
BstF5I GGATG 4 cut(s) 273, 689, 1749, 1858
BstFNI CGCG 5 cut(s) 40, 120, 315, 321, 1719
BstH2I RGCGCY 2 cut(s) 270, 1503
BstHHI GCGC 7 cut(s) 42, 122, 269, 315, 321, 531, 1502
BstKTI GATC 8 cut(s) 181, 247, 559, 613, 712, 1108, 1198, 1795
BstMAI GTCTC 3 cut(s) 822, 1345, 1742
BstMBI GATC 8 cut(s) 178, 244, 556, 610, 709, 1105, 1195, 1792
BstMCI CGRYCG 1 cut(s) 1198
BstNI CCWGG 6 cut(s) 176, 393, 1245, 1494, 1544, 1740
BstNSI RCATGY 3 cut(s) 401, 1430, 1530
BstSCI CCNGG 6 cut(s) 174, 391, 1243, 1492, 1542, 1738
BstSFI CTRYAG 2 cut(s) 564, 633
BstUI CGCG 5 cut(s) 40, 120, 315, 321, 1719
BstV1I GCAGC 5 cut(s) 143, 513, 655, 885, 1828
BstX2I RGATCY 4 cut(s) 178, 709, 1105, 1792
BstXI CCANNNNNNTGG 1 cut(s) 1178
BstYI RGATCY 4 cut(s) 178, 709, 1105, 1792
Bsu15I ATCGAT 1 cut(s) 1476
BsuRI GGCC 4 cut(s) 10, 143, 1053, 1542
BsuTUI ATCGAT 1 cut(s) 1476
BtgI CCRYGG 3 cut(s) 223, 291, 356
BtgZI GCGATG 1 cut(s) 316
BtrI CACGTC 1 cut(s) 348
BtsCI GGATG 4 cut(s) 273, 689, 1749, 1858
BtsI GCAGTG 2 cut(s) 135, 1576
BtsIMutI CAGTG 4 cut(s) 135, 266, 1176, 1576
CaiI CAGNNNCTG 2 cut(s) 137, 572
CfoI GCGC 7 cut(s) 42, 122, 269, 315, 321, 531, 1502
Cfr13I GGNCC 7 cut(s) 8, 30, 220, 476, 1484, 1540, 1631
ClaI ATCGAT 1 cut(s) 1476
CseI GACGC 2 cut(s) 54, 244
Csp6I GTAC 5 cut(s) 156, 500, 1167, 1360, 1680
CviQI GTAC 5 cut(s) 156, 500, 1167, 1360, 1680
DdeI CTNAG 3 cut(s) 573, 1150, 1467
DinI GGCGCC 1 cut(s) 268
DpnI GATC 8 cut(s) 180, 246, 558, 612, 711, 1107, 1197, 1794
DpnII GATC 8 cut(s) 178, 244, 556, 610, 709, 1105, 1195, 1792
DrdI GACNNNNNNGTC 1 cut(s) 1516
DriI GACNNNNNGTC 1 cut(s) 1018
DseDI GACNNNNNNGTC 1 cut(s) 1516
EaeI YGGCCR 1 cut(s) 1051
Eam1104I CTCTTC 2 cut(s) 58, 1416
Eam1105I GACNNNNNGTC 1 cut(s) 1018
EarI CTCTTC 2 cut(s) 58, 1416
EciI GGCGGA 1 cut(s) 245
Eco130I CCWWGG 4 cut(s) 291, 339, 356, 838
Eco47I GGWCC 5 cut(s) 30, 220, 476, 1484, 1631
Eco47III AGCGCT 1 cut(s) 1501
Eco72I CACGTG 2 cut(s) 101, 1580
Eco88I CYCGRG 2 cut(s) 308, 1780
EcoNI CCTNNNNNAGG 1 cut(s) 1491
EcoO109I RGGNCCY 3 cut(s) 1484, 1540, 1631
EcoRII CCWGG 6 cut(s) 174, 391, 1243, 1492, 1542, 1738
EcoT14I CCWWGG 4 cut(s) 291, 339, 356, 838
EcoT22I ATGCAT 1 cut(s) 1599
EgeI GGCGCC 1 cut(s) 268
EheI GGCGCC 1 cut(s) 268
ErhI CCWWGG 4 cut(s) 291, 339, 356, 838
FalI AAGNNNNNCTT 2 cut(s) 465, 497
FaqI GGGAC 2 cut(s) 52, 1799
FauI CCCGC 3 cut(s) 417, 1311, 1886
FauNDI CATATG 2 cut(s) 981, 1753
Fnu4HI GCNGC 8 cut(s) 132, 141, 187, 265, 527, 644, 874, 1842
FokI GGATG 4 cut(s) 260, 696, 1756, 1865
Fsp4HI GCNGC 8 cut(s) 132, 141, 187, 265, 527, 644, 874, 1842
FspBI CTAG 6 cut(s) 153, 647, 1512, 1700, 1796, 1845
GlaI GCGC 7 cut(s) 41, 121, 268, 314, 320, 530, 1501
GluI GCNGC 8 cut(s) 132, 141, 187, 265, 527, 644, 874, 1842
GsaI CCCAGC 2 cut(s) 471, 1032
GsuI CTGGAG 3 cut(s) 942, 1023, 1200
HaeII RGCGCY 2 cut(s) 270, 1503
HaeIII GGCC 4 cut(s) 10, 143, 1053, 1542
HapII CCGG 2 cut(s) 855, 1507
HgaI GACGC 2 cut(s) 54, 244
HhaI GCGC 7 cut(s) 42, 122, 269, 315, 321, 531, 1502
Hin1I GRCGYC 1 cut(s) 267
Hin6I GCGC 7 cut(s) 40, 120, 267, 313, 319, 529, 1500
HinP1I GCGC 7 cut(s) 40, 120, 267, 313, 319, 529, 1500
HincII GTYRAC 2 cut(s) 1804, 1981
HindII GTYRAC 2 cut(s) 1804, 1981
HinfI GANTC 6 cut(s) 296, 917, 1142, 1211, 1256, 1888
HpaI GTTAAC 1 cut(s) 1981
HpaII CCGG 2 cut(s) 855, 1507
HphI GGTGA 5 cut(s) 425, 694, 899, 1022, 1335
Hpy166II GTNNAC 3 cut(s) 220, 1804, 1981
Hpy188I TCNGA 5 cut(s) 183, 574, 1216, 1255, 1921
Hpy188III TCNNGA 8 cut(s) 738, 788, 921, 1160, 1648, 1796, 1892, 1973
Hpy8I GTNNAC 3 cut(s) 220, 1804, 1981
Hpy99I CGWCG 1 cut(s) 238
HpyAV CCTTC 8 cut(s) 133, 355, 467, 674, 935, 940, 964, 1316
HpyCH4III ACNGT 5 cut(s) 172, 1060, 1096, 1335, 1956
HpyCH4IV ACGT 6 cut(s) 100, 198, 347, 1579, 1837, 1977
HpyF3I CTNAG 3 cut(s) 573, 1150, 1467
HpySE526I ACGT 6 cut(s) 100, 198, 347, 1579, 1837, 1977
Hsp92I GRCGYC 1 cut(s) 267
HspAI GCGC 7 cut(s) 40, 120, 267, 313, 319, 529, 1500
KasI GGCGCC 1 cut(s) 266
KpnI GGTACC 1 cut(s) 1170
KspAI GTTAAC 1 cut(s) 1981
Kzo9I GATC 8 cut(s) 178, 244, 556, 610, 709, 1105, 1195, 1792
LguI GCTCTTC 1 cut(s) 1416
LmnI GCTCC 3 cut(s) 258, 640, 1004
Lsp1109I GCAGC 5 cut(s) 143, 513, 655, 885, 1828
LweI GCATC 5 cut(s) 113, 454, 712, 739, 1606
MaeI CTAG 6 cut(s) 153, 647, 1512, 1700, 1796, 1845
MaeII ACGT 6 cut(s) 100, 198, 347, 1579, 1837, 1977
MalI GATC 8 cut(s) 180, 246, 558, 612, 711, 1107, 1197, 1794
MbiI CCGCTC 1 cut(s) 424
MboI GATC 8 cut(s) 178, 244, 556, 610, 709, 1105, 1195, 1792
MboII GAAGA 9 cut(s) 45, 530, 533, 858, 950, 1409, 1433, 1907, 1975
MfeI CAATTG 1 cut(s) 1392
MflI RGATCY 4 cut(s) 178, 709, 1105, 1792
MhlI GDGCHC 1 cut(s) 1779
MlsI TGGCCA 1 cut(s) 1053
MluCI AATT 8 cut(s) 324, 351, 450, 933, 990, 1188, 1302, 1392
MluNI TGGCCA 1 cut(s) 1053
Mly113I GGCGCC 1 cut(s) 267
MlyI GAGTC 3 cut(s) 290, 1136, 1882
MmeI TCCRAC 1 cut(s) 1083
Mox20I TGGCCA 1 cut(s) 1053
Mph1103I ATGCAT 1 cut(s) 1599
MscI TGGCCA 1 cut(s) 1053
MseI TTAA 7 cut(s) 92, 515, 1233, 1301, 1610, 1958, 1980
Msp20I TGGCCA 1 cut(s) 1053
MspA1I CMGCKG 1 cut(s) 1208
MspI CCGG 2 cut(s) 855, 1507
MspR9I CCNGG 6 cut(s) 176, 393, 1245, 1494, 1544, 1740
MunI CAATTG 1 cut(s) 1392
Mva1269I GAATGC 1 cut(s) 876
MvaI CCWGG 6 cut(s) 176, 393, 1245, 1494, 1544, 1740
MvnI CGCG 5 cut(s) 40, 120, 315, 321, 1719
NarI GGCGCC 1 cut(s) 267
NcoI CCATGG 2 cut(s) 291, 356
NdeI CATATG 2 cut(s) 981, 1753
NdeII GATC 8 cut(s) 178, 244, 556, 610, 709, 1105, 1195, 1792
NheI GCTAGC 1 cut(s) 1844
NlaIV GGNNCC 8 cut(s) 9, 180, 268, 795, 1107, 1168, 1485, 1541
NmeAIII GCCGAG 1 cut(s) 107
NmuCI GTSAC 7 cut(s) 343, 735, 887, 908, 1010, 1571, 1732
NsiI ATGCAT 1 cut(s) 1599
NspI RCATGY 3 cut(s) 401, 1430, 1530
NspV TTCGAA 1 cut(s) 1329
PaeR7I CTCGAG 2 cut(s) 308, 1780
PciI ACATGT 1 cut(s) 1526
PciSI GCTCTTC 1 cut(s) 1416
PctI GAATGC 1 cut(s) 876
PfeI GAWTC 3 cut(s) 917, 1211, 1256
PflMI CCANNNNNTGG 2 cut(s) 808, 1049
PfoI TCCNGGA 2 cut(s) 174, 1492
PkrI GCNGC 8 cut(s) 133, 142, 188, 266, 528, 645, 875, 1843
Ple19I CGATCG 1 cut(s) 1198
PleI GAGTC 3 cut(s) 290, 1136, 1882
PluTI GGCGCC 1 cut(s) 270
PmaCI CACGTG 2 cut(s) 101, 1580
PmlI CACGTG 2 cut(s) 101, 1580
PpsI GAGTC 3 cut(s) 290, 1136, 1882
Ppu21I YACGTR 3 cut(s) 101, 1580, 1838
PpuMI RGGWCCY 2 cut(s) 1484, 1631
PscI ACATGT 1 cut(s) 1526
PshBI ATTAAT 1 cut(s) 1233
PsiI TTATAA 1 cut(s) 758
Psp5II RGGWCCY 2 cut(s) 1484, 1631
Psp6I CCWGG 6 cut(s) 174, 391, 1243, 1492, 1542, 1738
PspCI CACGTG 2 cut(s) 101, 1580
PspFI CCCAGC 2 cut(s) 467, 1028
PspGI CCWGG 6 cut(s) 174, 391, 1243, 1492, 1542, 1738
PspN4I GGNNCC 8 cut(s) 9, 180, 268, 795, 1107, 1168, 1485, 1541
PspPI GGNCC 7 cut(s) 8, 30, 220, 476, 1484, 1540, 1631
PspPPI RGGWCCY 2 cut(s) 1484, 1631
PspXI VCTCGAGB 2 cut(s) 308, 1780
PstI CTGCAG 1 cut(s) 568
PstNI CAGNNNCTG 2 cut(s) 137, 572
PsuI RGATCY 4 cut(s) 178, 709, 1105, 1792
PvuI CGATCG 1 cut(s) 1198
PvuII CAGCTG 1 cut(s) 1208
RsaI GTAC 5 cut(s) 157, 501, 1168, 1361, 1681
RsaNI GTAC 5 cut(s) 156, 500, 1167, 1360, 1680
SapI GCTCTTC 1 cut(s) 1416
SaqAI TTAA 7 cut(s) 92, 515, 1233, 1301, 1610, 1958, 1980
SatI GCNGC 8 cut(s) 132, 141, 187, 265, 527, 644, 874, 1842
Sau3AI GATC 8 cut(s) 178, 244, 556, 610, 709, 1105, 1195, 1792
Sau96I GGNCC 7 cut(s) 8, 30, 220, 476, 1484, 1540, 1631
ScaI AGTACT 1 cut(s) 157
SchI GAGTC 3 cut(s) 290, 1136, 1882
ScrFI CCNGG 6 cut(s) 176, 393, 1245, 1494, 1544, 1740
SduI GDGCHC 1 cut(s) 1779
SfaNI GCATC 5 cut(s) 113, 454, 712, 739, 1606
SfcI CTRYAG 2 cut(s) 564, 633
SfoI GGCGCC 1 cut(s) 268
Sfr274I CTCGAG 2 cut(s) 308, 1780
SfuI TTCGAA 1 cut(s) 1329
SinI GGWCC 5 cut(s) 30, 220, 476, 1484, 1631
SlaI CTCGAG 2 cut(s) 308, 1780
SmlI CTYRAG 4 cut(s) 308, 788, 1385, 1780
SmoI CTYRAG 4 cut(s) 308, 788, 1385, 1780
Sse9I AATT 8 cut(s) 324, 351, 450, 933, 990, 1188, 1302, 1392
SspDI GGCGCC 1 cut(s) 266
SspI AATATT 1 cut(s) 849
SspMI CTAG 6 cut(s) 153, 647, 1512, 1700, 1796, 1845
StyD4I CCNGG 6 cut(s) 174, 391, 1243, 1492, 1542, 1738
StyI CCWWGG 4 cut(s) 291, 339, 356, 838
TaaI ACNGT 5 cut(s) 172, 1060, 1096, 1335, 1956
TaiI ACGT 6 cut(s) 103, 201, 350, 1582, 1840, 1980
TaqI TCGA 6 cut(s) 309, 1198, 1329, 1476, 1627, 1781
TasI AATT 8 cut(s) 324, 351, 450, 933, 990, 1188, 1302, 1392
TatI WGTACW 2 cut(s) 155, 1359
TauI GCSGC 3 cut(s) 143, 189, 267
TfiI GAWTC 3 cut(s) 917, 1211, 1256
Tru1I TTAA 7 cut(s) 92, 515, 1233, 1301, 1610, 1958, 1980
Tru9I TTAA 7 cut(s) 92, 515, 1233, 1301, 1610, 1958, 1980
TscAI CASTG 4 cut(s) 142, 266, 1183, 1576
TseFI GTSAC 7 cut(s) 343, 735, 887, 908, 1010, 1571, 1732
TseI GCWGC 5 cut(s) 131, 526, 643, 873, 1841
Tsp45I GTSAC 7 cut(s) 343, 735, 887, 908, 1010, 1571, 1732
TspDTI ATGAA 4 cut(s) 603, 887, 977, 1434
TspRI CASTG 4 cut(s) 142, 266, 1183, 1576
Van91I CCANNNNNTGG 2 cut(s) 808, 1049
VpaK11BI GGWCC 5 cut(s) 30, 220, 476, 1484, 1631
VspI ATTAAT 1 cut(s) 1233
XagI CCTNNNNNAGG 1 cut(s) 1491
XbaI TCTAGA 1 cut(s) 1795
XceI RCATGY 3 cut(s) 401, 1430, 1530
XcmI CCANNNNNNNNNTGG 1 cut(s) 1099
XhoI CTCGAG 2 cut(s) 308, 1780
XspI CTAG 6 cut(s) 153, 647, 1512, 1700, 1796, 1845
ZrmI AGTACT 1 cut(s) 157
Zsp2I ATGCAT 1 cut(s) 1599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.