FvH4_6g27370

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0013219
Physical Location & Seq
Reverse (-)
20948255 .. 20951789
3535 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g27370.t1

Sequence Viewer

Length: 522 bp
ATGGTAGCCGGTCGAAAATGGGCTGTTTGCTTGACAGACAACTCAACCTCTCCTTGTTCCCTCGATGTCCCTGACCCTCCTGGCTTCTCTAGGGCCTCCTCTGAGCAGGATGATTCAACCTTGAGTCGCCAAAAGAAAGAAGCTGAATCAACTTGGAAAGCTCAGAAAGCTTGGGAAGTCGCGCAGGCCCCTTTTAAGAATTTGTTAATGATGGGTTTTATGATGTGGATGGCTGGCAGCACAGTCCACTTGTTCAGCATTGGAATTACATTTTCTGCTCTCTGGCAGCCCATAAGTGCCTTACAAGGTGTAGGAAAGGTTTTTGAGCCCTACAAGGACAGGAAAGTGGATCTTCTTGCCCCAAAGTTGCTCTTCATTGCCCTTAATTTGGGGGGTTTAGCACTTGGTGTCTGGAAGCTTAACACATTGGGGTTGCTTCCTACACATGCATCCGATTGGGTTTCATCCTTACCTCCTTCACAGGAGGTTGAGTATTCTGGTGGTGGCATTCCTCTGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1077

Protein Analysis

174

Amino Acids

18.87

Weight (kDa)

6.72

Isoelectric Point (pI)

37.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMC4 PF06417 45 - 157 2.9e-39 ER membrane protein complex subunit 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013219)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10780
fragaria_vesca FvH4_3g24420 FvH4_6g27370
malus_domestica MD08G1052600.v1.1 MD15G1042900.v1.1
prunus_persica Prupe.1G396200_v2.0.a1
pyrus_communis pycom08g04390 pycom15g04000
rosa_chinensis RchiOBHm_Chr6g0299241
rosa_laevigata RLG00000011450
rosa_multiflora Rmu_sc0001434.1_g000007
rosa_roxburghii Rroxscaffold_7G00168860
rosa_rugosa Rorug06G0286500
rosa_samantha Rh6AG397800 Rh6BG406300 Rh6CG412400 Rh6DG399100
rosa_wichuraiana Rw6G034820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 182
AclWI GGATC 1 cut(s) 357
AcsI RAATTY 1 cut(s) 199
AdeI CACNNNGTG 1 cut(s) 407
AfaI GTAC 1 cut(s) 518
AfiI CCNNNNNNNGG 1 cut(s) 388
AgsI TTSAA 1 cut(s) 117
AjnI CCWGG 1 cut(s) 79
AluBI AGCT 4 cut(s) 143, 161, 170, 418
AluI AGCT 4 cut(s) 143, 161, 170, 418
AlwI GGATC 1 cut(s) 357
AoxI GGCC 2 cut(s) 93, 186
ApeKI GCWGC 2 cut(s) 237, 286
ApoI RAATTY 1 cut(s) 199
AspLEI GCGC 1 cut(s) 184
AspS9I GGNCC 2 cut(s) 93, 187
BanII GRGCYC 1 cut(s) 330
BbvI GCAGC 2 cut(s) 249, 298
BccI CCATC 2 cut(s) 205, 223
BciT130I CCWGG 1 cut(s) 81
BfaI CTAG 1 cut(s) 90
BisI GCNGC 2 cut(s) 238, 287
BlsI GCNGC 2 cut(s) 239, 288
Bme1390I CCNGG 1 cut(s) 81
BmgT120I GGNCC 2 cut(s) 93, 187
BmiI GGNNCC 1 cut(s) 189
BmrFI CCNGG 1 cut(s) 81
BmsI GCATC 1 cut(s) 458
BpuEI CTTGAG 1 cut(s) 142
BsaXI ACNNNNNCTCC 2 cut(s) 476, 506
Bsc4I CCNNNNNNNGG 1 cut(s) 388
Bse118I RCCGGY 1 cut(s) 8
Bse3DI GCAATG 1 cut(s) 375
BseBI CCWGG 1 cut(s) 81
BseGI GGATG 4 cut(s) 115, 234, 449, 464
BseLI CCNNNNNNNGG 1 cut(s) 388
BseMI GCAATG 1 cut(s) 375
BseMII CTCAG 2 cut(s) 93, 176
BseRI GAGGAG 1 cut(s) 88
BseXI GCAGC 2 cut(s) 249, 298
Bsh1236I CGCG 1 cut(s) 182
Bsh1285I CGRYCG 1 cut(s) 13
BshFI GGCC 2 cut(s) 95, 188
BsiEI CGRYCG 1 cut(s) 13
BsiSI CCGG 1 cut(s) 9
BslFI GGGAC 1 cut(s) 53
BslI CCNNNNNNNGG 1 cut(s) 388
BsmFI GGGAC 1 cut(s) 53
BsmI GAATGC 1 cut(s) 507
BsnI GGCC 2 cut(s) 95, 188
Bsp1286I GDGCHC 1 cut(s) 330
Bsp143I GATC 1 cut(s) 349
BspANI GGCC 2 cut(s) 95, 188
BspCNI CTCAG 2 cut(s) 94, 175
BspFNI CGCG 1 cut(s) 182
BspLI GGNNCC 1 cut(s) 189
BspPI GGATC 1 cut(s) 357
BspQI GCTCTTC 1 cut(s) 377
BsrDI GCAATG 1 cut(s) 375
BsrFI RCCGGY 1 cut(s) 8
BssAI RCCGGY 1 cut(s) 8
BssMI GATC 1 cut(s) 349
Bst2UI CCWGG 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 244
Bst6I CTCTTC 1 cut(s) 377
BstC8I GCNNGC 2 cut(s) 186, 235
BstDEI CTNAG 2 cut(s) 102, 162
BstF5I GGATG 4 cut(s) 115, 234, 449, 464
BstFNI CGCG 1 cut(s) 182
BstHHI GCGC 1 cut(s) 184
BstKTI GATC 1 cut(s) 352
BstMBI GATC 1 cut(s) 349
BstMCI CGRYCG 1 cut(s) 13
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstNI CCWGG 1 cut(s) 81
BstNSI RCATGY 1 cut(s) 449
BstSCI CCNGG 1 cut(s) 79
BstUI CGCG 1 cut(s) 182
BstV1I GCAGC 2 cut(s) 249, 298
BstX2I RGATCY 1 cut(s) 349
BstYI RGATCY 1 cut(s) 349
BsuRI GGCC 2 cut(s) 95, 188
BtsCI GGATG 4 cut(s) 115, 234, 449, 464
Cac8I GCNNGC 2 cut(s) 186, 235
CfoI GCGC 1 cut(s) 184
Cfr10I RCCGGY 1 cut(s) 8
Cfr13I GGNCC 2 cut(s) 93, 187
Csp6I GTAC 1 cut(s) 517
CviAII CATG 1 cut(s) 446
CviQI GTAC 1 cut(s) 517
DdeI CTNAG 2 cut(s) 102, 162
DpnI GATC 1 cut(s) 351
DpnII GATC 1 cut(s) 349
DraIII CACNNNGTG 1 cut(s) 407
Eam1104I CTCTTC 1 cut(s) 377
EarI CTCTTC 1 cut(s) 377
Eco24I GRGCYC 1 cut(s) 330
EcoO109I RGGNCCY 2 cut(s) 93, 187
EcoRII CCWGG 1 cut(s) 79
EcoT22I ATGCAT 1 cut(s) 451
EcoT38I GRGCYC 1 cut(s) 330
FaeI CATG 1 cut(s) 449
FaiI YATR 3 cut(s) 221, 293, 447
FalI AAGNNNNNCTT 4 cut(s) 336, 368, 356, 388
FaqI GGGAC 1 cut(s) 53
FatI CATG 1 cut(s) 445
Fnu4HI GCNGC 2 cut(s) 238, 287
FokI GGATG 4 cut(s) 122, 241, 436, 451
FriOI GRGCYC 1 cut(s) 330
Fsp4HI GCNGC 2 cut(s) 238, 287
FspBI CTAG 1 cut(s) 90
GlaI GCGC 1 cut(s) 183
GluI GCNGC 2 cut(s) 238, 287
HaeIII GGCC 2 cut(s) 95, 188
HapII CCGG 1 cut(s) 9
HhaI GCGC 1 cut(s) 184
Hin1II CATG 1 cut(s) 449
Hin6I GCGC 1 cut(s) 182
HinP1I GCGC 1 cut(s) 182
HindIII AAGCTT 2 cut(s) 168, 416
HinfI GANTC 3 cut(s) 113, 124, 146
HpaII CCGG 1 cut(s) 9
Hpy166II GTNNAC 1 cut(s) 247
Hpy188I TCNGA 3 cut(s) 103, 165, 454
Hpy188III TCNNGA 1 cut(s) 412
Hpy8I GTNNAC 1 cut(s) 247
HpyAV CCTTC 1 cut(s) 486
HpyCH4III ACNGT 1 cut(s) 244
HpyCH4V TGCA 1 cut(s) 449
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
HpyF3I CTNAG 2 cut(s) 102, 162
Hsp92II CATG 1 cut(s) 449
HspAI GCGC 1 cut(s) 182
Kzo9I GATC 1 cut(s) 349
LguI GCTCTTC 1 cut(s) 377
Lsp1109I GCAGC 2 cut(s) 249, 298
LweI GCATC 1 cut(s) 458
MaeI CTAG 1 cut(s) 90
MalI GATC 1 cut(s) 351
MboI GATC 1 cut(s) 349
MboII GAAGA 2 cut(s) 344, 364
MflI RGATCY 1 cut(s) 349
MhlI GDGCHC 1 cut(s) 330
MluCI AATT 3 cut(s) 199, 264, 385
MlyI GAGTC 1 cut(s) 133
MnlI CCTC 8 cut(s) 58, 71, 87, 106, 109, 478, 483, 522
Mph1103I ATGCAT 1 cut(s) 451
MseI TTAA 4 cut(s) 195, 206, 384, 420
MspI CCGG 1 cut(s) 9
MspR9I CCNGG 1 cut(s) 81
Mva1269I GAATGC 1 cut(s) 507
MvaI CCWGG 1 cut(s) 81
MvnI CGCG 1 cut(s) 182
MwoI GCNNNNNNNGC 1 cut(s) 167
NdeII GATC 1 cut(s) 349
NlaIII CATG 1 cut(s) 449
NlaIV GGNNCC 1 cut(s) 189
NsiI ATGCAT 1 cut(s) 451
NspI RCATGY 1 cut(s) 449
PciSI GCTCTTC 1 cut(s) 377
PctI GAATGC 1 cut(s) 507
PfeI GAWTC 2 cut(s) 113, 146
PkrI GCNGC 2 cut(s) 239, 288
PleI GAGTC 1 cut(s) 132
PpsI GAGTC 1 cut(s) 132
Psp6I CCWGG 1 cut(s) 79
PspGI CCWGG 1 cut(s) 79
PspN4I GGNNCC 1 cut(s) 189
PspPI GGNCC 2 cut(s) 93, 187
PsuI RGATCY 1 cut(s) 349
RsaI GTAC 1 cut(s) 518
RsaNI GTAC 1 cut(s) 517
SapI GCTCTTC 1 cut(s) 377
SaqAI TTAA 4 cut(s) 195, 206, 384, 420
SatI GCNGC 2 cut(s) 238, 287
Sau3AI GATC 1 cut(s) 349
Sau96I GGNCC 2 cut(s) 93, 187
SchI GAGTC 1 cut(s) 133
ScrFI CCNGG 1 cut(s) 81
SduI GDGCHC 1 cut(s) 330
SfaNI GCATC 1 cut(s) 458
SmlI CTYRAG 1 cut(s) 121
SmoI CTYRAG 1 cut(s) 121
Sse9I AATT 3 cut(s) 199, 264, 385
SspMI CTAG 1 cut(s) 90
StyD4I CCNGG 1 cut(s) 79
TaaI ACNGT 1 cut(s) 244
TaqI TCGA 2 cut(s) 13, 63
TasI AATT 3 cut(s) 199, 264, 385
TatI WGTACW 1 cut(s) 516
TfiI GAWTC 2 cut(s) 113, 146
Tru1I TTAA 4 cut(s) 195, 206, 384, 420
Tru9I TTAA 4 cut(s) 195, 206, 384, 420
TseI GCWGC 2 cut(s) 237, 286
TspDTI ATGAA 2 cut(s) 364, 453
XapI RAATTY 1 cut(s) 199
XceI RCATGY 1 cut(s) 449
XspI CTAG 1 cut(s) 90
Zsp2I ATGCAT 1 cut(s) 451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.