MD08G1052600.v1.1

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0013219
Physical Location & Seq
Forward (+)
4123757 .. 4126247
2491 bp
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UTR
Exon/CDS
Intron
MD08G1052600.v1.1.491

Sequence Viewer

Length: 498 bp
ATGGAGAAGGGAAAGGGAGTGATGGGAGCAGGTCGGAGATGGGCCGTTGACTTGACTGACAACTCAACGTCCCCTTCTTCCCGCGACTTCCCCGATCCTCCTGGCTTCTCTCGCGCCTCCCTCGAACAGGATGATTCGACTAAAGCTTGGGAAGTTGCACAGGCCCCAGTCAAGAATTTGATGATGATGGGTTTCATGATGTGGATGGCTGGGAGCACGGTGCATTTGTTTAGCATTGGAATTACGTTTTCCGCTCTTTGGCAGCCCATAAGCGCCTTACAGGGTGTAGGGAAGGTTTTTGAGCCCTACAAGGACGGCAAAGTAGATCTTATTGCCCCAAAGTTGCTCTTCATTGCCCTTAATCTGGGGGGTTTAGCACTGGGTGTTTGGAAGCTTAACACTTTGGGGTTGCTTCCTACACATGCATCTGATTGGGTTTCTTCCTTACCCCCTGCTCAGGAGGTTGAGTATTCGGGTGGTGGCATTCTTCGTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1077

Protein Analysis

166

Amino Acids

17.84

Weight (kDa)

6.08

Isoelectric Point (pI)

41.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMC4 PF06417 42 - 149 4.4e-37 ER membrane protein complex subunit 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013219)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10780
fragaria_vesca FvH4_3g24420 FvH4_6g27370
malus_domestica MD08G1052600.v1.1 MD15G1042900.v1.1
prunus_persica Prupe.1G396200_v2.0.a1
pyrus_communis pycom08g04390 pycom15g04000
rosa_chinensis RchiOBHm_Chr6g0299241
rosa_laevigata RLG00000011450
rosa_multiflora Rmu_sc0001434.1_g000007
rosa_roxburghii Rroxscaffold_7G00168860
rosa_rugosa Rorug06G0286500
rosa_samantha Rh6AG397800 Rh6BG406300 Rh6CG412400 Rh6DG399100
rosa_wichuraiana Rw6G034820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 20
AccBSI CCGCTC 1 cut(s) 254
AccII CGCG 2 cut(s) 84, 114
AciI CCGC 2 cut(s) 82, 252
AclWI GGATC 1 cut(s) 89
AcsI RAATTY 1 cut(s) 175
AdeI CACNNNGTG 1 cut(s) 383
AfiI CCNNNNNNNGG 4 cut(s) 127, 258, 364, 457
AjnI CCWGG 1 cut(s) 100
AluBI AGCT 2 cut(s) 146, 394
AluI AGCT 2 cut(s) 146, 394
Alw21I GWGCWC 1 cut(s) 218
AlwI GGATC 1 cut(s) 89
AoxI GGCC 2 cut(s) 42, 162
ApeKI GCWGC 1 cut(s) 262
ApoI RAATTY 1 cut(s) 175
AspLEI GCGC 2 cut(s) 116, 275
AspS9I GGNCC 2 cut(s) 42, 163
BanII GRGCYC 1 cut(s) 306
Bbv12I GWGCWC 1 cut(s) 218
BbvI GCAGC 1 cut(s) 274
BccI CCATC 4 cut(s) 16, 33, 181, 199
BceAI ACGGC 2 cut(s) 29, 331
BciT130I CCWGG 1 cut(s) 102
BfoI RGCGCY 1 cut(s) 276
BfuAI ACCTGC 1 cut(s) 20
BglII AGATCT 1 cut(s) 325
BisI GCNGC 1 cut(s) 263
BlsI GCNGC 1 cut(s) 264
Bme1390I CCNGG 1 cut(s) 102
BmgT120I GGNCC 2 cut(s) 42, 163
BmiI GGNNCC 1 cut(s) 165
BmrFI CCNGG 1 cut(s) 102
BmrI ACTGGG 2 cut(s) 161, 389
BmsI GCATC 1 cut(s) 434
BmuI ACTGGG 2 cut(s) 161, 389
Bpu10I CCTNAGC 1 cut(s) 456
BsaXI ACNNNNNCTCC 2 cut(s) 452, 482
Bsc4I CCNNNNNNNGG 4 cut(s) 127, 258, 364, 457
Bse1I ACTGG 2 cut(s) 167, 384
Bse3DI GCAATG 1 cut(s) 351
BseBI CCWGG 1 cut(s) 102
BseGI GGATG 2 cut(s) 136, 210
BseLI CCNNNNNNNGG 4 cut(s) 127, 258, 364, 457
BseMI GCAATG 1 cut(s) 351
BseMII CTCAG 1 cut(s) 470
BseNI ACTGG 2 cut(s) 167, 384
BseXI GCAGC 1 cut(s) 274
BseYI CCCAGC 1 cut(s) 209
Bsh1236I CGCG 2 cut(s) 84, 114
BshFI GGCC 2 cut(s) 44, 164
BsiHKAI GWGCWC 1 cut(s) 218
BslFI GGGAC 1 cut(s) 55
BslI CCNNNNNNNGG 4 cut(s) 127, 258, 364, 457
BsmFI GGGAC 1 cut(s) 55
BsmI GAATGC 1 cut(s) 483
BsnI GGCC 2 cut(s) 44, 164
Bsp1286I GDGCHC 2 cut(s) 218, 306
Bsp143I GATC 2 cut(s) 94, 325
BspACI CCGC 2 cut(s) 82, 252
BspANI GGCC 2 cut(s) 44, 164
BspCNI CTCAG 1 cut(s) 469
BspFNI CGCG 2 cut(s) 84, 114
BspHI TCATGA 1 cut(s) 195
BspLI GGNNCC 1 cut(s) 165
BspMI ACCTGC 1 cut(s) 20
BspPI GGATC 1 cut(s) 89
BspQI GCTCTTC 1 cut(s) 353
BsrBI CCGCTC 1 cut(s) 254
BsrDI GCAATG 1 cut(s) 351
BsrI ACTGG 2 cut(s) 167, 384
BssMI GATC 2 cut(s) 94, 325
Bst2UI CCWGG 1 cut(s) 102
Bst4CI ACNGT 1 cut(s) 220
Bst6I CTCTTC 1 cut(s) 353
BstDEI CTNAG 1 cut(s) 456
BstENI CCTNNNNNAGG 1 cut(s) 125
BstF5I GGATG 2 cut(s) 136, 210
BstFNI CGCG 2 cut(s) 84, 114
BstH2I RGCGCY 1 cut(s) 276
BstHHI GCGC 2 cut(s) 116, 275
BstKTI GATC 2 cut(s) 97, 328
BstMBI GATC 2 cut(s) 94, 325
BstMWI GCNNNNNNNGC 1 cut(s) 111
BstNI CCWGG 1 cut(s) 102
BstNSI RCATGY 1 cut(s) 425
BstSCI CCNGG 1 cut(s) 100
BstUI CGCG 2 cut(s) 84, 114
BstV1I GCAGC 1 cut(s) 274
BstX2I RGATCY 1 cut(s) 325
BstYI RGATCY 1 cut(s) 325
BsuRI GGCC 2 cut(s) 44, 164
BtsCI GGATG 2 cut(s) 136, 210
BtsIMutI CAGTG 1 cut(s) 377
BveI ACCTGC 1 cut(s) 20
CciI TCATGA 1 cut(s) 195
CfoI GCGC 2 cut(s) 116, 275
Cfr13I GGNCC 2 cut(s) 42, 163
CviAII CATG 2 cut(s) 196, 422
CviJI RGCY 8 cut(s) 44, 105, 146, 164, 209, 265, 304, 394
CviKI_1 RGCY 8 cut(s) 44, 105, 146, 164, 209, 265, 304, 394
DdeI CTNAG 1 cut(s) 456
DpnI GATC 2 cut(s) 96, 327
DpnII GATC 2 cut(s) 94, 325
DraIII CACNNNGTG 1 cut(s) 383
Eam1104I CTCTTC 1 cut(s) 353
EarI CTCTTC 1 cut(s) 353
Eco24I GRGCYC 1 cut(s) 306
EcoNI CCTNNNNNAGG 1 cut(s) 125
EcoO109I RGGNCCY 1 cut(s) 163
EcoRII CCWGG 1 cut(s) 100
EcoT22I ATGCAT 1 cut(s) 427
EcoT38I GRGCYC 1 cut(s) 306
FaeI CATG 2 cut(s) 199, 425
FaiI YATR 3 cut(s) 197, 269, 423
FalI AAGNNNNNCTT 4 cut(s) 312, 344, 332, 364
FaqI GGGAC 1 cut(s) 55
FatI CATG 2 cut(s) 195, 421
FauI CCCGC 1 cut(s) 89
Fnu4HI GCNGC 1 cut(s) 263
FokI GGATG 2 cut(s) 143, 217
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 1 cut(s) 263
GlaI GCGC 2 cut(s) 115, 274
GluI GCNGC 1 cut(s) 263
GsaI CCCAGC 1 cut(s) 213
HaeII RGCGCY 1 cut(s) 276
HaeIII GGCC 2 cut(s) 44, 164
HhaI GCGC 2 cut(s) 116, 275
Hin1II CATG 2 cut(s) 199, 425
Hin6I GCGC 2 cut(s) 114, 273
HinP1I GCGC 2 cut(s) 114, 273
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HindIII AAGCTT 2 cut(s) 144, 392
HinfI GANTC 1 cut(s) 134
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 2 cut(s) 36, 430
Hpy188III TCNNGA 3 cut(s) 172, 196, 458
Hpy8I GTNNAC 1 cut(s) 49
HpyAV CCTTC 2 cut(s) 84, 286
HpyCH4III ACNGT 1 cut(s) 220
HpyCH4IV ACGT 2 cut(s) 68, 245
HpyCH4V TGCA 3 cut(s) 158, 223, 425
HpyF10VI GCNNNNNNNGC 1 cut(s) 111
HpyF3I CTNAG 1 cut(s) 456
HpySE526I ACGT 2 cut(s) 68, 245
Hsp92II CATG 2 cut(s) 199, 425
HspAI GCGC 2 cut(s) 114, 273
Kzo9I GATC 2 cut(s) 94, 325
LguI GCTCTTC 1 cut(s) 353
LmnI GCTCC 2 cut(s) 26, 213
Lsp1109I GCAGC 1 cut(s) 274
LweI GCATC 1 cut(s) 434
MaeII ACGT 2 cut(s) 68, 245
MalI GATC 2 cut(s) 96, 327
MbiI CCGCTC 1 cut(s) 254
MboI GATC 2 cut(s) 94, 325
MboII GAAGA 4 cut(s) 69, 340, 432, 479
MflI RGATCY 1 cut(s) 325
MhlI GDGCHC 2 cut(s) 218, 306
MluCI AATT 2 cut(s) 175, 240
MmeI TCCRAC 1 cut(s) 14
MnlI CCTC 4 cut(s) 108, 127, 131, 454
Mph1103I ATGCAT 1 cut(s) 427
MseI TTAA 2 cut(s) 360, 396
MspR9I CCNGG 1 cut(s) 102
Mva1269I GAATGC 1 cut(s) 483
MvaI CCWGG 1 cut(s) 102
MvnI CGCG 2 cut(s) 84, 114
MwoI GCNNNNNNNGC 1 cut(s) 111
NdeII GATC 2 cut(s) 94, 325
NlaIII CATG 2 cut(s) 199, 425
NlaIV GGNNCC 1 cut(s) 165
NsiI ATGCAT 1 cut(s) 427
NspI RCATGY 1 cut(s) 425
PagI TCATGA 1 cut(s) 195
PciSI GCTCTTC 1 cut(s) 353
PctI GAATGC 1 cut(s) 483
PfeI GAWTC 1 cut(s) 134
PkrI GCNGC 1 cut(s) 264
Psp6I CCWGG 1 cut(s) 100
PspFI CCCAGC 1 cut(s) 209
PspGI CCWGG 1 cut(s) 100
PspN4I GGNNCC 1 cut(s) 165
PspPI GGNCC 2 cut(s) 42, 163
PsuI RGATCY 1 cut(s) 325
SapI GCTCTTC 1 cut(s) 353
SaqAI TTAA 2 cut(s) 360, 396
SatI GCNGC 1 cut(s) 263
Sau3AI GATC 2 cut(s) 94, 325
Sau96I GGNCC 2 cut(s) 42, 163
ScrFI CCNGG 1 cut(s) 102
SduI GDGCHC 2 cut(s) 218, 306
SetI ASST 7 cut(s) 34, 71, 148, 248, 297, 396, 465
SfaNI GCATC 1 cut(s) 434
Sse9I AATT 2 cut(s) 175, 240
SsiI CCGC 2 cut(s) 82, 252
StyD4I CCNGG 1 cut(s) 100
TaaI ACNGT 1 cut(s) 220
TaiI ACGT 2 cut(s) 71, 248
TaqI TCGA 2 cut(s) 123, 137
TasI AATT 2 cut(s) 175, 240
TfiI GAWTC 1 cut(s) 134
Tru1I TTAA 2 cut(s) 360, 396
Tru9I TTAA 2 cut(s) 360, 396
TscAI CASTG 1 cut(s) 384
TseI GCWGC 1 cut(s) 262
TspDTI ATGAA 2 cut(s) 184, 340
TspRI CASTG 1 cut(s) 384
XagI CCTNNNNNAGG 1 cut(s) 125
XapI RAATTY 1 cut(s) 175
XceI RCATGY 1 cut(s) 425
Zsp2I ATGCAT 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.