pycom08g04390

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0013219
Physical Location & Seq
Forward (+)
3378400 .. 3381479
3080 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g04390.1

Sequence Viewer

Length: 588 bp
ATGAATGGAAATCTCAGGGAAGTTGTAAAGACGACGACGGCGACGATGGAGAAGGGAAAGGGAGTGATGGGAGCAGGTCGGAGATGGGCCGTTGACTTGACTGACAACTCAACTTCCCCTTCTTCCCGCGACTTCCCCGACCCTCCTGGCTTCTCTCGCGCCTCCCTCGAACAGGATGATTCGACTGTGAGTCGCCAGAAGAAGGATGCTGAATCTACTTGGAAAGCTCAGAAAGCTTGGGAAGTTGCACAGGCCCCATTCAAGAATTTGATGATGATGGGTTTCATGATGTGGATGGCTGGGAGCACGGTGCATTTGTTTAGCATTGGAATTACGTTTTCCGCTCTTTGGCAGCCCATAAGCGCCTTACAGGGTGTAGGGAAGGTTTTTGAGCCCTACAAGGACGGCAAAGTAGATCTTATTGCCCCAAAGTTGCTCTTCATTGCCCTTAATTTGGGGGGTTTAGCACTGGGTGTTTGGAAGCTTAACACTTTGGGGTTGCTTCCTACGCATGCATCTGATTGGGTTTCTTCCTTACCCCCTGCTCAGGAGGTTGAGTATTCGGGTGGTGGCATTCTTCGTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1077

Protein Analysis

196

Amino Acids

21.25

Weight (kDa)

8.83

Isoelectric Point (pI)

37.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMC4 PF06417 67 - 179 9.4e-39 ER membrane protein complex subunit 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013219)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10780
fragaria_vesca FvH4_3g24420 FvH4_6g27370
malus_domestica MD08G1052600.v1.1 MD15G1042900.v1.1
prunus_persica Prupe.1G396200_v2.0.a1
pyrus_communis pycom08g04390 pycom15g04000
rosa_chinensis RchiOBHm_Chr6g0299241
rosa_laevigata RLG00000011450
rosa_multiflora Rmu_sc0001434.1_g000007
rosa_roxburghii Rroxscaffold_7G00168860
rosa_rugosa Rorug06G0286500
rosa_samantha Rh6AG397800 Rh6BG406300 Rh6CG412400 Rh6DG399100
rosa_wichuraiana Rw6G034820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 65
AccBSI CCGCTC 1 cut(s) 344
AccII CGCG 2 cut(s) 129, 159
AciI CCGC 2 cut(s) 127, 342
AcsI RAATTY 1 cut(s) 265
AdeI CACNNNGTG 1 cut(s) 473
AfiI CCNNNNNNNGG 5 cut(s) 172, 202, 348, 454, 547
AgsI TTSAA 1 cut(s) 262
AhdI GACNNNNNGTC 1 cut(s) 189
AjnI CCWGG 1 cut(s) 145
AluBI AGCT 3 cut(s) 227, 236, 484
AluI AGCT 3 cut(s) 227, 236, 484
Alw21I GWGCWC 1 cut(s) 308
AoxI GGCC 2 cut(s) 87, 252
ApeKI GCWGC 1 cut(s) 352
ApoI RAATTY 1 cut(s) 265
AspLEI GCGC 2 cut(s) 161, 365
AspS9I GGNCC 2 cut(s) 87, 253
BanII GRGCYC 1 cut(s) 396
Bbv12I GWGCWC 1 cut(s) 308
BbvI GCAGC 1 cut(s) 364
BccI CCATC 5 cut(s) 40, 61, 78, 271, 289
BceAI ACGGC 3 cut(s) 54, 74, 421
BciT130I CCWGG 1 cut(s) 147
BfoI RGCGCY 1 cut(s) 366
BfuAI ACCTGC 1 cut(s) 65
BglII AGATCT 1 cut(s) 415
BisI GCNGC 1 cut(s) 353
BlsI GCNGC 1 cut(s) 354
Bme1390I CCNGG 1 cut(s) 147
BmeRI GACNNNNNGTC 1 cut(s) 189
BmgT120I GGNCC 2 cut(s) 87, 253
BmiI GGNNCC 1 cut(s) 255
BmrFI CCNGG 1 cut(s) 147
BmrI ACTGGG 1 cut(s) 479
BmsI GCATC 2 cut(s) 196, 524
BmuI ACTGGG 1 cut(s) 479
Bpu10I CCTNAGC 1 cut(s) 546
BsaXI ACNNNNNCTCC 2 cut(s) 542, 572
Bsc4I CCNNNNNNNGG 5 cut(s) 172, 202, 348, 454, 547
Bse1I ACTGG 1 cut(s) 474
Bse3DI GCAATG 1 cut(s) 441
BseBI CCWGG 1 cut(s) 147
BseGI GGATG 3 cut(s) 181, 211, 300
BseLI CCNNNNNNNGG 5 cut(s) 172, 202, 348, 454, 547
BseMI GCAATG 1 cut(s) 441
BseMII CTCAG 3 cut(s) 28, 242, 560
BseNI ACTGG 1 cut(s) 474
BseXI GCAGC 1 cut(s) 364
BseYI CCCAGC 1 cut(s) 299
Bsh1236I CGCG 2 cut(s) 129, 159
BshFI GGCC 2 cut(s) 89, 254
BsiHKAI GWGCWC 1 cut(s) 308
BslI CCNNNNNNNGG 5 cut(s) 172, 202, 348, 454, 547
BsmI GAATGC 1 cut(s) 573
BsnI GGCC 2 cut(s) 89, 254
Bsp1286I GDGCHC 2 cut(s) 308, 396
Bsp143I GATC 1 cut(s) 415
BspACI CCGC 2 cut(s) 127, 342
BspANI GGCC 2 cut(s) 89, 254
BspCNI CTCAG 3 cut(s) 27, 241, 559
BspFNI CGCG 2 cut(s) 129, 159
BspHI TCATGA 1 cut(s) 285
BspLI GGNNCC 1 cut(s) 255
BspMI ACCTGC 1 cut(s) 65
BspQI GCTCTTC 1 cut(s) 443
BsrBI CCGCTC 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 441
BsrI ACTGG 1 cut(s) 474
BssMI GATC 1 cut(s) 415
Bst2UI CCWGG 1 cut(s) 147
Bst4CI ACNGT 2 cut(s) 187, 310
Bst6I CTCTTC 1 cut(s) 443
BstC8I GCNNGC 1 cut(s) 513
BstDEI CTNAG 3 cut(s) 14, 228, 546
BstENI CCTNNNNNAGG 1 cut(s) 170
BstF5I GGATG 3 cut(s) 181, 211, 300
BstFNI CGCG 2 cut(s) 129, 159
BstH2I RGCGCY 1 cut(s) 366
BstHHI GCGC 2 cut(s) 161, 365
BstKTI GATC 1 cut(s) 418
BstMBI GATC 1 cut(s) 415
BstMWI GCNNNNNNNGC 3 cut(s) 156, 233, 508
BstNI CCWGG 1 cut(s) 147
BstNSI RCATGY 1 cut(s) 515
BstSCI CCNGG 1 cut(s) 145
BstUI CGCG 2 cut(s) 129, 159
BstV1I GCAGC 1 cut(s) 364
BstX2I RGATCY 1 cut(s) 415
BstYI RGATCY 1 cut(s) 415
BsuRI GGCC 2 cut(s) 89, 254
BtsCI GGATG 3 cut(s) 181, 211, 300
BtsIMutI CAGTG 1 cut(s) 467
BveI ACCTGC 1 cut(s) 65
Cac8I GCNNGC 1 cut(s) 513
CciI TCATGA 1 cut(s) 285
CfoI GCGC 2 cut(s) 161, 365
Cfr13I GGNCC 2 cut(s) 87, 253
CviAII CATG 2 cut(s) 286, 512
CviJI RGCY 9 cut(s) 89, 150, 227, 236, 254, 299, 355, 394, 484
CviKI_1 RGCY 9 cut(s) 89, 150, 227, 236, 254, 299, 355, 394, 484
DdeI CTNAG 3 cut(s) 14, 228, 546
DpnI GATC 1 cut(s) 417
DpnII GATC 1 cut(s) 415
DraIII CACNNNGTG 1 cut(s) 473
DriI GACNNNNNGTC 1 cut(s) 189
Eam1104I CTCTTC 1 cut(s) 443
Eam1105I GACNNNNNGTC 1 cut(s) 189
EarI CTCTTC 1 cut(s) 443
Eco24I GRGCYC 1 cut(s) 396
EcoNI CCTNNNNNAGG 1 cut(s) 170
EcoO109I RGGNCCY 1 cut(s) 253
EcoRII CCWGG 1 cut(s) 145
EcoT22I ATGCAT 1 cut(s) 517
EcoT38I GRGCYC 1 cut(s) 396
FaeI CATG 2 cut(s) 289, 515
FaiI YATR 3 cut(s) 287, 359, 513
FalI AAGNNNNNCTT 4 cut(s) 402, 434, 422, 454
FatI CATG 2 cut(s) 285, 511
FauI CCCGC 1 cut(s) 134
Fnu4HI GCNGC 1 cut(s) 353
FokI GGATG 3 cut(s) 188, 218, 307
FriOI GRGCYC 1 cut(s) 396
Fsp4HI GCNGC 1 cut(s) 353
GlaI GCGC 2 cut(s) 160, 364
GluI GCNGC 1 cut(s) 353
GsaI CCCAGC 1 cut(s) 303
HaeII RGCGCY 1 cut(s) 366
HaeIII GGCC 2 cut(s) 89, 254
HhaI GCGC 2 cut(s) 161, 365
Hin1II CATG 2 cut(s) 289, 515
Hin6I GCGC 2 cut(s) 159, 363
HinP1I GCGC 2 cut(s) 159, 363
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HindIII AAGCTT 2 cut(s) 234, 482
HinfI GANTC 3 cut(s) 179, 190, 212
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 3 cut(s) 81, 231, 520
Hpy188III TCNNGA 3 cut(s) 262, 286, 548
Hpy8I GTNNAC 1 cut(s) 94
Hpy99I CGWCG 3 cut(s) 37, 40, 46
HpyAV CCTTC 4 cut(s) 46, 129, 196, 376
HpyCH4III ACNGT 2 cut(s) 187, 310
HpyCH4IV ACGT 1 cut(s) 335
HpyCH4V TGCA 3 cut(s) 248, 313, 515
HpyF10VI GCNNNNNNNGC 3 cut(s) 156, 233, 508
HpyF3I CTNAG 3 cut(s) 14, 228, 546
HpySE526I ACGT 1 cut(s) 335
Hsp92II CATG 2 cut(s) 289, 515
HspAI GCGC 2 cut(s) 159, 363
Kzo9I GATC 1 cut(s) 415
LguI GCTCTTC 1 cut(s) 443
LmnI GCTCC 2 cut(s) 71, 303
Lsp1109I GCAGC 1 cut(s) 364
LweI GCATC 2 cut(s) 196, 524
MaeII ACGT 1 cut(s) 335
MalI GATC 1 cut(s) 417
MbiI CCGCTC 1 cut(s) 344
MboI GATC 1 cut(s) 415
MboII GAAGA 5 cut(s) 114, 211, 430, 522, 569
MflI RGATCY 1 cut(s) 415
MhlI GDGCHC 2 cut(s) 308, 396
MluCI AATT 3 cut(s) 265, 330, 451
MlyI GAGTC 1 cut(s) 199
MmeI TCCRAC 1 cut(s) 59
MnlI CCTC 4 cut(s) 153, 172, 176, 544
Mph1103I ATGCAT 1 cut(s) 517
MseI TTAA 2 cut(s) 450, 486
MspR9I CCNGG 1 cut(s) 147
Mva1269I GAATGC 1 cut(s) 573
MvaI CCWGG 1 cut(s) 147
MvnI CGCG 2 cut(s) 129, 159
MwoI GCNNNNNNNGC 3 cut(s) 156, 233, 508
NdeII GATC 1 cut(s) 415
NlaIII CATG 2 cut(s) 289, 515
NlaIV GGNNCC 1 cut(s) 255
NsiI ATGCAT 1 cut(s) 517
NspI RCATGY 1 cut(s) 515
PaeI GCATGC 1 cut(s) 515
PagI TCATGA 1 cut(s) 285
PciSI GCTCTTC 1 cut(s) 443
PcsI WCGNNNNNNNCGW 2 cut(s) 38, 41
PctI GAATGC 1 cut(s) 573
PfeI GAWTC 2 cut(s) 179, 212
PkrI GCNGC 1 cut(s) 354
PleI GAGTC 1 cut(s) 198
PpsI GAGTC 1 cut(s) 198
Psp6I CCWGG 1 cut(s) 145
PspFI CCCAGC 1 cut(s) 299
PspGI CCWGG 1 cut(s) 145
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 2 cut(s) 87, 253
PsuI RGATCY 1 cut(s) 415
SapI GCTCTTC 1 cut(s) 443
SaqAI TTAA 2 cut(s) 450, 486
SatI GCNGC 1 cut(s) 353
Sau3AI GATC 1 cut(s) 415
Sau96I GGNCC 2 cut(s) 87, 253
SchI GAGTC 1 cut(s) 199
ScrFI CCNGG 1 cut(s) 147
SduI GDGCHC 2 cut(s) 308, 396
SetI ASST 7 cut(s) 79, 229, 238, 338, 387, 486, 555
SfaNI GCATC 2 cut(s) 196, 524
SphI GCATGC 1 cut(s) 515
Sse9I AATT 3 cut(s) 265, 330, 451
SsiI CCGC 2 cut(s) 127, 342
StyD4I CCNGG 1 cut(s) 145
TaaI ACNGT 2 cut(s) 187, 310
TaiI ACGT 1 cut(s) 338
TaqI TCGA 2 cut(s) 168, 182
TasI AATT 3 cut(s) 265, 330, 451
TfiI GAWTC 2 cut(s) 179, 212
Tru1I TTAA 2 cut(s) 450, 486
Tru9I TTAA 2 cut(s) 450, 486
TscAI CASTG 1 cut(s) 474
TseI GCWGC 1 cut(s) 352
TspDTI ATGAA 3 cut(s) 17, 274, 430
TspRI CASTG 1 cut(s) 474
XagI CCTNNNNNAGG 1 cut(s) 170
XapI RAATTY 1 cut(s) 265
XceI RCATGY 1 cut(s) 515
Zsp2I ATGCAT 1 cut(s) 517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.