RLG00000011450

ER membrane protein complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0013219
Physical Location & Seq
Reverse (-)
9516839 .. 9518293
1455 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011450

Sequence Viewer

Length: 543 bp
ATGGAGAAGGGAAAGGGAGTTATGGGAGCCGGTCGAAGATGGGCCGTTGACTTGACGGACAACTCAACCTCTCCTTGTTCCCGCGACGTCCCTGACCCTCCTGGCTTCTCCAGGGCCTCCTCCGATCAGGACGATTCAACTGTGAGTCGCCAGAAGAAGGAAGCTGAATCAACATGGAAATCTCAGAAAGCTTGGGAAGTTGCACAGGCCCCTTTTAAGAATTTGTTAATGATGGGCTTCATGATGTGGATGGCTGGCAGCACAGTCCATTTATTCAGCATTGGAATTACATTTTCTGCTCTCTGGCAGCCCATAAGTGCCTTGCAAGGTGTAGGAAAGGTTTTTGAGCCCTACAAGGACAGCAAAGTGGATCTTCTTGGCCCAAAGTTGCTCTTCATCGCCCTTAATTTGGGGGGTTTGGCACTTGGTGTCTGGAAGCTTAACACATTGGGGTTGCTTCCTACACATGCATCCGACTGGGTTTCATCCTTACCCCCGGCTCAGGAGGTTGAGTATTCTGGTGGCGGCATTCCTCTCCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1077

Protein Analysis

181

Amino Acids

19.51

Weight (kDa)

6.83

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EMC4 PF06417 52 - 164 3.9e-39 ER membrane protein complex subunit 4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013219)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G10780
fragaria_vesca FvH4_3g24420 FvH4_6g27370
malus_domestica MD08G1052600.v1.1 MD15G1042900.v1.1
prunus_persica Prupe.1G396200_v2.0.a1
pyrus_communis pycom08g04390 pycom15g04000
rosa_chinensis RchiOBHm_Chr6g0299241
rosa_laevigata RLG00000011450
rosa_multiflora Rmu_sc0001434.1_g000007
rosa_roxburghii Rroxscaffold_7G00168860
rosa_rugosa Rorug06G0286500
rosa_samantha Rh6AG397800 Rh6BG406300 Rh6CG412400 Rh6DG399100
rosa_wichuraiana Rw6G034820

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 90
AccII CGCG 1 cut(s) 84
AciI CCGC 2 cut(s) 82, 525
AclWI GGATC 1 cut(s) 378
AcsI RAATTY 1 cut(s) 220
AcyI GRCGYC 1 cut(s) 87
AdeI CACNNNGTG 1 cut(s) 428
AfiI CCNNNNNNNGG 3 cut(s) 157, 409, 502
AgsI TTSAA 1 cut(s) 138
AjnI CCWGG 2 cut(s) 100, 110
AluBI AGCT 3 cut(s) 164, 191, 439
AluI AGCT 3 cut(s) 164, 191, 439
AlwI GGATC 1 cut(s) 378
AoxI GGCC 4 cut(s) 42, 114, 207, 379
ApeKI GCWGC 2 cut(s) 258, 307
ApoI RAATTY 1 cut(s) 220
AspS9I GGNCC 4 cut(s) 42, 114, 208, 380
AsuC2I CCSGG 1 cut(s) 497
BanII GRGCYC 1 cut(s) 351
BbvI GCAGC 2 cut(s) 270, 319
BccI CCATC 3 cut(s) 33, 226, 244
BceAI ACGGC 1 cut(s) 29
BciT130I CCWGG 2 cut(s) 102, 112
BcnI CCSGG 1 cut(s) 497
BisI GCNGC 3 cut(s) 259, 308, 526
BlsI GCNGC 3 cut(s) 260, 309, 527
Bme1390I CCNGG 3 cut(s) 102, 112, 497
BmgT120I GGNCC 4 cut(s) 42, 114, 208, 380
BmiI GGNNCC 2 cut(s) 28, 210
BmrFI CCNGG 3 cut(s) 102, 112, 497
BmrI ACTGGG 1 cut(s) 487
BmsI GCATC 1 cut(s) 479
BmuI ACTGGG 1 cut(s) 487
BpmI CTGGAG 1 cut(s) 94
Bpu10I CCTNAGC 1 cut(s) 501
BpuMI CCSGG 1 cut(s) 497
BsaHI GRCGYC 1 cut(s) 87
BsaJI CCNNGG 2 cut(s) 111, 495
BsaXI ACNNNNNCTCC 2 cut(s) 497, 527
Bsc4I CCNNNNNNNGG 3 cut(s) 157, 409, 502
Bse118I RCCGGY 1 cut(s) 29
Bse1I ACTGG 1 cut(s) 482
BseBI CCWGG 2 cut(s) 102, 112
BseDI CCNNGG 2 cut(s) 111, 495
BseGI GGATG 3 cut(s) 255, 470, 485
BseLI CCNNNNNNNGG 3 cut(s) 157, 409, 502
BseMII CTCAG 2 cut(s) 197, 515
BseNI ACTGG 1 cut(s) 482
BseRI GAGGAG 1 cut(s) 109
BseXI GCAGC 2 cut(s) 270, 319
Bsh1236I CGCG 1 cut(s) 84
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 4 cut(s) 44, 116, 209, 381
BsiEI CGRYCG 1 cut(s) 34
BsiSI CCGG 2 cut(s) 30, 497
BslFI GGGAC 1 cut(s) 74
BslI CCNNNNNNNGG 3 cut(s) 157, 409, 502
BsmFI GGGAC 1 cut(s) 74
BsmI GAATGC 1 cut(s) 528
BsnI GGCC 4 cut(s) 44, 116, 209, 381
Bsp1286I GDGCHC 1 cut(s) 351
Bsp143I GATC 2 cut(s) 124, 370
BspACI CCGC 2 cut(s) 82, 525
BspANI GGCC 4 cut(s) 44, 116, 209, 381
BspCNI CTCAG 2 cut(s) 196, 514
BspFNI CGCG 1 cut(s) 84
BspHI TCATGA 1 cut(s) 240
BspLI GGNNCC 2 cut(s) 28, 210
BspPI GGATC 1 cut(s) 378
BspQI GCTCTTC 1 cut(s) 398
BsrFI RCCGGY 1 cut(s) 29
BsrI ACTGG 1 cut(s) 482
BssAI RCCGGY 1 cut(s) 29
BssECI CCNNGG 2 cut(s) 111, 495
BssMI GATC 2 cut(s) 124, 370
BssNI GRCGYC 1 cut(s) 87
Bst2UI CCWGG 2 cut(s) 102, 112
Bst4CI ACNGT 2 cut(s) 142, 265
Bst6I CTCTTC 1 cut(s) 398
BstACI GRCGYC 1 cut(s) 87
BstC8I GCNNGC 1 cut(s) 256
BstDEI CTNAG 2 cut(s) 183, 501
BstF5I GGATG 3 cut(s) 255, 470, 485
BstFNI CGCG 1 cut(s) 84
BstKTI GATC 2 cut(s) 127, 373
BstMBI GATC 2 cut(s) 124, 370
BstMCI CGRYCG 1 cut(s) 34
BstNI CCWGG 2 cut(s) 102, 112
BstNSI RCATGY 1 cut(s) 470
BstSCI CCNGG 3 cut(s) 100, 110, 495
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 2 cut(s) 270, 319
BstX2I RGATCY 1 cut(s) 370
BstYI RGATCY 1 cut(s) 370
BsuRI GGCC 4 cut(s) 44, 116, 209, 381
BtgZI GCGATG 1 cut(s) 382
BtsCI GGATG 3 cut(s) 255, 470, 485
Cac8I GCNNGC 1 cut(s) 256
CciI TCATGA 1 cut(s) 240
Cfr10I RCCGGY 1 cut(s) 29
Cfr13I GGNCC 4 cut(s) 42, 114, 208, 380
CviAII CATG 3 cut(s) 174, 241, 467
DdeI CTNAG 2 cut(s) 183, 501
DpnI GATC 2 cut(s) 126, 372
DpnII GATC 2 cut(s) 124, 370
DraIII CACNNNGTG 1 cut(s) 428
Eam1104I CTCTTC 1 cut(s) 398
EarI CTCTTC 1 cut(s) 398
Eco24I GRGCYC 1 cut(s) 351
EcoO109I RGGNCCY 2 cut(s) 114, 208
EcoRII CCWGG 2 cut(s) 100, 110
EcoT22I ATGCAT 1 cut(s) 472
EcoT38I GRGCYC 1 cut(s) 351
FaeI CATG 3 cut(s) 177, 244, 470
FaiI YATR 5 cut(s) 23, 175, 242, 314, 468
FalI AAGNNNNNCTT 4 cut(s) 357, 389, 377, 409
FaqI GGGAC 1 cut(s) 74
FatI CATG 3 cut(s) 173, 240, 466
FauI CCCGC 1 cut(s) 89
Fnu4HI GCNGC 3 cut(s) 259, 308, 526
FokI GGATG 3 cut(s) 262, 457, 472
FriOI GRGCYC 1 cut(s) 351
Fsp4HI GCNGC 3 cut(s) 259, 308, 526
GluI GCNGC 3 cut(s) 259, 308, 526
GsuI CTGGAG 1 cut(s) 94
HaeIII GGCC 4 cut(s) 44, 116, 209, 381
HapII CCGG 2 cut(s) 30, 497
Hin1I GRCGYC 1 cut(s) 87
Hin1II CATG 3 cut(s) 177, 244, 470
HincII GTYRAC 1 cut(s) 49
HindII GTYRAC 1 cut(s) 49
HindIII AAGCTT 2 cut(s) 189, 437
HinfI GANTC 3 cut(s) 134, 145, 167
HpaII CCGG 2 cut(s) 30, 497
Hpy166II GTNNAC 1 cut(s) 49
Hpy188I TCNGA 3 cut(s) 124, 186, 475
Hpy188III TCNNGA 4 cut(s) 128, 241, 433, 503
Hpy8I GTNNAC 1 cut(s) 49
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 1 cut(s) 151
HpyCH4III ACNGT 2 cut(s) 142, 265
HpyCH4IV ACGT 1 cut(s) 87
HpyCH4V TGCA 3 cut(s) 203, 325, 470
HpyF3I CTNAG 2 cut(s) 183, 501
HpySE526I ACGT 1 cut(s) 87
Hsp92I GRCGYC 1 cut(s) 87
Hsp92II CATG 3 cut(s) 177, 244, 470
Kzo9I GATC 2 cut(s) 124, 370
LguI GCTCTTC 1 cut(s) 398
LmnI GCTCC 1 cut(s) 26
Lsp1109I GCAGC 2 cut(s) 270, 319
LweI GCATC 1 cut(s) 479
MaeII ACGT 1 cut(s) 87
MalI GATC 2 cut(s) 126, 372
MboI GATC 2 cut(s) 124, 370
MboII GAAGA 4 cut(s) 48, 166, 365, 385
MflI RGATCY 1 cut(s) 370
MhlI GDGCHC 1 cut(s) 351
MluCI AATT 3 cut(s) 220, 285, 406
MlyI GAGTC 1 cut(s) 154
MmeI TCCRAC 1 cut(s) 498
MnlI CCTC 6 cut(s) 79, 108, 127, 130, 499, 543
Mph1103I ATGCAT 1 cut(s) 472
MseI TTAA 4 cut(s) 216, 227, 405, 441
MspI CCGG 2 cut(s) 30, 497
MspR9I CCNGG 3 cut(s) 102, 112, 497
Mva1269I GAATGC 1 cut(s) 528
MvaI CCWGG 2 cut(s) 102, 112
MvnI CGCG 1 cut(s) 84
NciI CCSGG 1 cut(s) 497
NdeII GATC 2 cut(s) 124, 370
NlaIII CATG 3 cut(s) 177, 244, 470
NlaIV GGNNCC 2 cut(s) 28, 210
NsiI ATGCAT 1 cut(s) 472
NspI RCATGY 1 cut(s) 470
PagI TCATGA 1 cut(s) 240
PciSI GCTCTTC 1 cut(s) 398
PctI GAATGC 1 cut(s) 528
PfeI GAWTC 2 cut(s) 134, 167
PkrI GCNGC 3 cut(s) 260, 309, 527
PleI GAGTC 1 cut(s) 153
PpsI GAGTC 1 cut(s) 153
Psp6I CCWGG 2 cut(s) 100, 110
PspGI CCWGG 2 cut(s) 100, 110
PspN4I GGNNCC 2 cut(s) 28, 210
PspPI GGNCC 4 cut(s) 42, 114, 208, 380
PsuI RGATCY 1 cut(s) 370
SapI GCTCTTC 1 cut(s) 398
SaqAI TTAA 4 cut(s) 216, 227, 405, 441
SatI GCNGC 3 cut(s) 259, 308, 526
Sau3AI GATC 2 cut(s) 124, 370
Sau96I GGNCC 4 cut(s) 42, 114, 208, 380
SchI GAGTC 1 cut(s) 154
ScrFI CCNGG 3 cut(s) 102, 112, 497
SduI GDGCHC 1 cut(s) 351
SetI ASST 8 cut(s) 71, 90, 166, 193, 331, 342, 441, 510
SfaNI GCATC 1 cut(s) 479
Sse9I AATT 3 cut(s) 220, 285, 406
SsiI CCGC 2 cut(s) 82, 525
StyD4I CCNGG 3 cut(s) 100, 110, 495
TaaI ACNGT 2 cut(s) 142, 265
TaiI ACGT 1 cut(s) 90
TaqI TCGA 1 cut(s) 34
TasI AATT 3 cut(s) 220, 285, 406
TauI GCSGC 1 cut(s) 528
TfiI GAWTC 2 cut(s) 134, 167
Tru1I TTAA 4 cut(s) 216, 227, 405, 441
Tru9I TTAA 4 cut(s) 216, 227, 405, 441
TseI GCWGC 2 cut(s) 258, 307
TspDTI ATGAA 3 cut(s) 229, 385, 474
TspGWI ACGGA 1 cut(s) 71
XapI RAATTY 1 cut(s) 220
XceI RCATGY 1 cut(s) 470
ZraI GACGTC 1 cut(s) 88
Zsp2I ATGCAT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.