FvH4_6g29980

Rhomboid-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016892
Physical Location & Seq
Forward (+)
23178798 .. 23181515
2718 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g29980.t1

Sequence Viewer

Length: 1158 bp
ATGTACATACCTAATTCAACTGTCAACCCAACAAACAAGACTCGTTCCTTGCCACCCGAAATCTATATCTCCCCCTTCTCTCCATCCCCTACGACTTGGTGCACATTTTGCAGCTGCCACCATCGCCGAAACAGCTTTGGTGCAGTGTGTACCTATTCCTCCTTTGCGGCCGGCCGGTACCGATTGACATCCAAAGATATGTATTATCCACCAGCTCCGCCTCCAATGTATTATGCACCACCACCCCCGCCGCCCGCGCAGTTTTACGCCCCACCGCCCTTGCCACCACCCCAAAGGCGACACTCTCCGTGGCTTGTGCCTCTCATATGCTTGGTTAATCTCGGCATGTTTGTGTACGAAATGTATAAGAACAATTGCCCCTCTAGAAACTCAAAGGATACGTGTATGTTAGGTCATGTTTTTGGCAGGTTCTCATTCGAACCCTTCGATCAAGTTCATTGGATTGGTCCTACCGCAGATACTATTAGGAGCTTAGGAGGGCTCGATCGGAACCTGGTGGTGCATGGTCAAGCATGGCGCCTTTTCACTAGCATGTGGCTTCATGGTGGAGTCATTCATTTGCTTTTCAACACTATCAGCCTTGATTTCGTTGGACTCCGACTCGAGCGCGACTTCGGATATCATAGATTCGGGCTTGTGTATATGCTTTCTGGGGTGGGAGGAAGTGTAGCGTCTTGCCTTCGTATTGTTCAAGAAAACCTGCAATCGACTGTGTCTGCTGGCGCATCCGGGGCAATTTTTGGACTGTGTGGAGCCTCCCTTTCGGAGTTGATCACAAACTGGACACTCTATGATGATAAGTTCGCGGCCATCATGTTACTCCTTCTCAATGTTGGCGTGAACATCGGCCTTGGATTTCTACTGAAAATGGACAATTCAGCTCATATAGGAGGTTTCGTAGCTGGGTTCTTCCTCGGTTTTGTTTTCTTCATCAAGCCTCAGTTTGGATACATAAGCAGCAAGTTTATTCCATCATACCATGAGGTCCAACCTACACGAAGGCACAACTTCTGTCAATATTTCCTAGCGGTTCTAGGTCTTGTAGGCTCAATTGCTATATTCGCAGCTGGCTTCGGTCTGCTATTTAACATTAAGGAAATTAAGGAGAAGTTGCCGGATTCCGTCAAAAGTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

386

Amino Acids

42.91

Weight (kDa)

9.11

Isoelectric Point (pI)

57.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 176 - 317 1.1e-37 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016892)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g29980
malus_domestica MD00G1022400.v1.1 MD00G1143800.v1.1
prunus_persica Prupe.3G083700_v2.0.a1
pyrus_communis pycom17g21420
rosa_chinensis RchiOBHm_Chr2g0135991
rosa_laevigata RLG00000019495
rosa_roxburghii Rroxscaffold_2G00108710
rosa_rugosa Rorug02G0327200
rosa_samantha Rh2AG379200 Rh2BG385700 Rh2CG364800 Rh2DG402000
rosa_wichuraiana Rw2G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 417, 729
Acc65I GGTACC 1 cut(s) 177
AccB1I GGYRCC 2 cut(s) 177, 537
AccII CGCG 3 cut(s) 257, 630, 827
AciI CCGC 9 cut(s) 167, 218, 248, 251, 255, 275, 474, 827, 1049
AcoI YGGCCR 3 cut(s) 168, 172, 828
AcyI GRCGYC 1 cut(s) 538
AfaI GTAC 4 cut(s) 5, 151, 179, 356
AfiI CCNNNNNNNGG 1 cut(s) 965
AflIII ACRYGT 1 cut(s) 401
AgsI TTSAA 3 cut(s) 18, 589, 713
AjnI CCWGG 1 cut(s) 513
AluBI AGCT 7 cut(s) 114, 135, 215, 492, 902, 923, 1088
AluI AGCT 7 cut(s) 114, 135, 215, 492, 902, 923, 1088
Alw21I GWGCWC 1 cut(s) 104
Alw44I GTGCAC 1 cut(s) 100
Ama87I CYCGRG 1 cut(s) 623
AoxI GGCC 4 cut(s) 168, 172, 828, 868
ApaLI GTGCAC 1 cut(s) 100
ApeKI GCWGC 4 cut(s) 111, 114, 978, 1085
Asp718I GGTACC 1 cut(s) 177
AspLEI GCGC 4 cut(s) 259, 540, 630, 746
AspS9I GGNCC 2 cut(s) 467, 1006
AsuC2I CCSGG 1 cut(s) 751
AsuII TTCGAA 1 cut(s) 438
AvaI CYCGRG 1 cut(s) 623
AvaII GGWCC 2 cut(s) 467, 1006
BaeGI GKGCMC 1 cut(s) 104
BanI GGYRCC 2 cut(s) 177, 537
BanII GRGCYC 1 cut(s) 504
Bbv12I GWGCWC 1 cut(s) 104
BbvI GCAGC 4 cut(s) 101, 123, 990, 1097
BccI CCATC 4 cut(s) 91, 129, 839, 1000
BciT130I CCWGG 1 cut(s) 515
BciVI GTATCC 2 cut(s) 391, 962
BclI TGATCA 1 cut(s) 792
BcnI CCSGG 1 cut(s) 751
BfaI CTAG 4 cut(s) 384, 549, 1046, 1055
BfoI RGCGCY 1 cut(s) 541
BfuAI ACCTGC 2 cut(s) 417, 729
BfuI GTATCC 2 cut(s) 391, 962
BisI GCNGC 7 cut(s) 112, 115, 168, 251, 828, 979, 1086
BlsI GCNGC 7 cut(s) 113, 116, 169, 252, 829, 980, 1087
Bme1390I CCNGG 2 cut(s) 515, 751
Bme18I GGWCC 2 cut(s) 467, 1006
BmeT110I CYCGRG 1 cut(s) 623
BmgT120I GGNCC 2 cut(s) 467, 1006
BmiI GGNNCC 4 cut(s) 179, 512, 539, 775
BmrFI CCNGG 2 cut(s) 515, 751
BmsI GCATC 1 cut(s) 755
Bpu10I CCTNAGC 1 cut(s) 493
Bpu14I TTCGAA 1 cut(s) 438
BpuMI CCSGG 1 cut(s) 751
BsaAI YACGTR 1 cut(s) 402
BsaBI GATNNNNATC 1 cut(s) 187
BsaHI GRCGYC 1 cut(s) 538
BsaJI CCNNGG 4 cut(s) 308, 750, 871, 934
BsaXI ACNNNNNCTCC 4 cut(s) 672, 702, 903, 933
Bsc4I CCNNNNNNNGG 1 cut(s) 965
Bse118I RCCGGY 2 cut(s) 170, 174
Bse1I ACTGG 1 cut(s) 806
Bse8I GATNNNNATC 1 cut(s) 187
BseBI CCWGG 1 cut(s) 515
BseDI CCNNGG 4 cut(s) 308, 750, 871, 934
BseGI GGATG 3 cut(s) 83, 188, 746
BseJI GATNNNNATC 1 cut(s) 187
BseLI CCNNNNNNNGG 1 cut(s) 965
BseMII CTCAG 1 cut(s) 974
BseNI ACTGG 1 cut(s) 806
BseSI GKGCMC 1 cut(s) 104
BseX3I CGGCCG 2 cut(s) 168, 172
BseXI GCAGC 4 cut(s) 101, 123, 990, 1097
BseYI CCCAGC 1 cut(s) 923
BsgI GTGCAG 1 cut(s) 162
Bsh1236I CGCG 3 cut(s) 257, 630, 827
Bsh1285I CGRYCG 3 cut(s) 171, 175, 508
BshFI GGCC 4 cut(s) 170, 174, 830, 870
BshNI GGYRCC 2 cut(s) 177, 537
BsiEI CGRYCG 3 cut(s) 171, 175, 508
BsiHKAI GWGCWC 1 cut(s) 104
BsiHKCI CYCGRG 1 cut(s) 623
BsiSI CCGG 4 cut(s) 171, 175, 750, 1136
BslI CCNNNNNNNGG 1 cut(s) 965
BsnI GGCC 4 cut(s) 170, 174, 830, 870
BsoBI CYCGRG 1 cut(s) 623
Bsp119I TTCGAA 1 cut(s) 438
Bsp1286I GDGCHC 2 cut(s) 104, 504
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 448, 505, 792
BspACI CCGC 9 cut(s) 167, 218, 248, 251, 255, 275, 474, 827, 1049
BspANI GGCC 4 cut(s) 170, 174, 830, 870
BspCNI CTCAG 1 cut(s) 973
BspFNI CGCG 3 cut(s) 257, 630, 827
BspLI GGNNCC 4 cut(s) 179, 512, 539, 775
BspMI ACCTGC 2 cut(s) 417, 729
BspT104I TTCGAA 1 cut(s) 438
BspT107I GGYRCC 2 cut(s) 177, 537
BsrFI RCCGGY 2 cut(s) 170, 174
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 1 cut(s) 806
BssAI RCCGGY 2 cut(s) 170, 174
BssECI CCNNGG 4 cut(s) 308, 750, 871, 934
BssMI GATC 3 cut(s) 448, 505, 792
BssNI GRCGYC 1 cut(s) 538
BssT1I CCWWGG 1 cut(s) 871
Bst2UI CCWGG 1 cut(s) 515
Bst4CI ACNGT 3 cut(s) 22, 733, 768
BstACI GRCGYC 1 cut(s) 538
BstAPI GCANNNNNTGC 1 cut(s) 108
BstAUI TGTACA 1 cut(s) 3
BstBAI YACGTR 1 cut(s) 402
BstBI TTCGAA 1 cut(s) 438
BstC8I GCNNGC 4 cut(s) 172, 255, 742, 1090
BstDEI CTNAG 2 cut(s) 493, 960
BstDSI CCRYGG 1 cut(s) 308
BstF5I GGATG 3 cut(s) 83, 188, 746
BstFNI CGCG 3 cut(s) 257, 630, 827
BstH2I RGCGCY 1 cut(s) 541
BstHHI GCGC 4 cut(s) 259, 540, 630, 746
BstKTI GATC 3 cut(s) 451, 508, 795
BstMBI GATC 3 cut(s) 448, 505, 792
BstMCI CGRYCG 3 cut(s) 171, 175, 508
BstMWI GCNNNNNNNGC 6 cut(s) 108, 123, 132, 256, 752, 1082
BstNI CCWGG 1 cut(s) 515
BstNSI RCATGY 2 cut(s) 349, 556
BstSCI CCNGG 2 cut(s) 513, 749
BstSLI GKGCMC 1 cut(s) 104
BstUI CGCG 3 cut(s) 257, 630, 827
BstV1I GCAGC 4 cut(s) 101, 123, 990, 1097
BstZI CGGCCG 2 cut(s) 168, 172
BsuI GTATCC 2 cut(s) 391, 962
BsuRI GGCC 4 cut(s) 170, 174, 830, 870
BtgI CCRYGG 1 cut(s) 308
BtgZI GCGATG 1 cut(s) 107
BtsCI GGATG 3 cut(s) 83, 188, 746
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 1 cut(s) 150
BveI ACCTGC 2 cut(s) 417, 729
Cac8I GCNNGC 4 cut(s) 172, 255, 742, 1090
CfoI GCGC 4 cut(s) 259, 540, 630, 746
Cfr10I RCCGGY 2 cut(s) 170, 174
Cfr13I GGNCC 2 cut(s) 467, 1006
CseI GACGC 1 cut(s) 681
CsiI ACCWGGT 1 cut(s) 513
Csp6I GTAC 4 cut(s) 4, 150, 178, 355
CspCI CAANNNNNGTGG 2 cut(s) 261, 296
CviAII CATG 8 cut(s) 346, 416, 524, 534, 553, 563, 835, 1001
CviQI GTAC 4 cut(s) 4, 150, 178, 355
DdeI CTNAG 2 cut(s) 493, 960
DinI GGCGCC 1 cut(s) 539
DpnI GATC 3 cut(s) 450, 507, 794
DpnII GATC 3 cut(s) 448, 505, 792
EaeI YGGCCR 3 cut(s) 168, 172, 828
EagI CGGCCG 2 cut(s) 168, 172
EciI GGCGGA 1 cut(s) 207
EclXI CGGCCG 2 cut(s) 168, 172
Eco130I CCWWGG 1 cut(s) 871
Eco24I GRGCYC 1 cut(s) 504
Eco32I GATATC 1 cut(s) 641
Eco47I GGWCC 2 cut(s) 467, 1006
Eco52I CGGCCG 2 cut(s) 168, 172
Eco88I CYCGRG 1 cut(s) 623
EcoRII CCWGG 1 cut(s) 513
EcoRV GATATC 1 cut(s) 641
EcoT14I CCWWGG 1 cut(s) 871
EcoT38I GRGCYC 1 cut(s) 504
EgeI GGCGCC 1 cut(s) 539
EheI GGCGCC 1 cut(s) 539
ErhI CCWWGG 1 cut(s) 871
FaeI CATG 8 cut(s) 349, 419, 527, 537, 556, 566, 838, 1004
FatI CATG 8 cut(s) 345, 415, 523, 533, 552, 562, 834, 1000
FauI CCCGC 2 cut(s) 255, 262
FauNDI CATATG 1 cut(s) 326
FbaI TGATCA 1 cut(s) 792
Fnu4HI GCNGC 7 cut(s) 112, 115, 168, 251, 828, 979, 1086
FokI GGATG 3 cut(s) 70, 175, 733
FriOI GRGCYC 1 cut(s) 504
FseI GGCCGGCC 1 cut(s) 174
Fsp4HI GCNGC 7 cut(s) 112, 115, 168, 251, 828, 979, 1086
FspBI CTAG 4 cut(s) 384, 549, 1046, 1055
GlaI GCGC 4 cut(s) 258, 539, 629, 745
GluI GCNGC 7 cut(s) 112, 115, 168, 251, 828, 979, 1086
GsaI CCCAGC 1 cut(s) 927
HaeII RGCGCY 1 cut(s) 541
HaeIII GGCC 4 cut(s) 170, 174, 830, 870
HapII CCGG 4 cut(s) 171, 175, 750, 1136
HgaI GACGC 1 cut(s) 681
HhaI GCGC 4 cut(s) 259, 540, 630, 746
Hin1I GRCGYC 1 cut(s) 538
Hin1II CATG 8 cut(s) 349, 419, 527, 537, 556, 566, 838, 1004
Hin6I GCGC 4 cut(s) 257, 538, 628, 744
HinP1I GCGC 4 cut(s) 257, 538, 628, 744
HincII GTYRAC 1 cut(s) 25
HindII GTYRAC 1 cut(s) 25
HinfI GANTC 6 cut(s) 40, 570, 615, 621, 648, 1139
HpaII CCGG 4 cut(s) 171, 175, 750, 1136
Hpy166II GTNNAC 5 cut(s) 25, 102, 150, 355, 862
Hpy188I TCNGA 4 cut(s) 510, 620, 638, 787
Hpy188III TCNNGA 2 cut(s) 384, 713
Hpy8I GTNNAC 5 cut(s) 25, 102, 150, 355, 862
HpyAV CCTTC 5 cut(s) 85, 454, 710, 854, 1014
HpyCH4III ACNGT 3 cut(s) 22, 733, 768
HpyCH4IV ACGT 1 cut(s) 401
HpyCH4V TGCA 6 cut(s) 102, 111, 143, 236, 523, 724
HpyF10VI GCNNNNNNNGC 6 cut(s) 108, 123, 132, 256, 752, 1082
HpyF3I CTNAG 2 cut(s) 493, 960
HpySE526I ACGT 1 cut(s) 401
Hsp92I GRCGYC 1 cut(s) 538
Hsp92II CATG 8 cut(s) 349, 419, 527, 537, 556, 566, 838, 1004
HspAI GCGC 4 cut(s) 257, 538, 628, 744
KasI GGCGCC 1 cut(s) 537
KpnI GGTACC 1 cut(s) 181
KroI GCCGGC 1 cut(s) 170
KroNI GCCGGC 1 cut(s) 172
Ksp22I TGATCA 1 cut(s) 792
Kzo9I GATC 3 cut(s) 448, 505, 792
LmnI GCTCC 3 cut(s) 220, 489, 773
Lsp1109I GCAGC 4 cut(s) 101, 123, 990, 1097
LweI GCATC 1 cut(s) 755
MabI ACCWGGT 1 cut(s) 513
MaeI CTAG 4 cut(s) 384, 549, 1046, 1055
MaeII ACGT 1 cut(s) 401
MaeIII GTNAC 1 cut(s) 837
MalI GATC 3 cut(s) 450, 507, 794
MboI GATC 3 cut(s) 448, 505, 792
MboII GAAGA 2 cut(s) 922, 940
MfeI CAATTG 2 cut(s) 373, 1071
MhlI GDGCHC 2 cut(s) 104, 504
MluCI AATT 6 cut(s) 13, 373, 756, 895, 1071, 1119
Mly113I GGCGCC 1 cut(s) 538
MlyI GAGTC 4 cut(s) 34, 579, 609, 615
MmeI TCCRAC 3 cut(s) 592, 643, 1033
MroNI GCCGGC 1 cut(s) 170
MseI TTAA 4 cut(s) 336, 1107, 1113, 1122
MslI CAYNNNNRTG 2 cut(s) 350, 551
MspA1I CMGCKG 2 cut(s) 114, 1088
MspI CCGG 4 cut(s) 171, 175, 750, 1136
MspR9I CCNGG 2 cut(s) 515, 751
MunI CAATTG 2 cut(s) 373, 1071
MvaI CCWGG 1 cut(s) 515
MvnI CGCG 3 cut(s) 257, 630, 827
MwoI GCNNNNNNNGC 6 cut(s) 108, 123, 132, 256, 752, 1082
NaeI GCCGGC 1 cut(s) 172
NarI GGCGCC 1 cut(s) 538
NciI CCSGG 1 cut(s) 751
NdeI CATATG 1 cut(s) 326
NdeII GATC 3 cut(s) 448, 505, 792
NgoMIV GCCGGC 1 cut(s) 170
NlaIII CATG 8 cut(s) 349, 419, 527, 537, 556, 566, 838, 1004
NlaIV GGNNCC 4 cut(s) 179, 512, 539, 775
NmeAIII GCCGAG 1 cut(s) 321
NspI RCATGY 2 cut(s) 349, 556
NspV TTCGAA 1 cut(s) 438
PaeR7I CTCGAG 1 cut(s) 623
PcsI WCGNNNNNNNCGW 1 cut(s) 444
PdiI GCCGGC 1 cut(s) 172
PfeI GAWTC 2 cut(s) 648, 1139
PflFI GACNNNGTC 1 cut(s) 733
PkrI GCNGC 7 cut(s) 113, 116, 169, 252, 829, 980, 1087
Ple19I CGATCG 1 cut(s) 508
PleI GAGTC 4 cut(s) 34, 578, 609, 615
PluTI GGCGCC 1 cut(s) 541
PpsI GAGTC 4 cut(s) 34, 578, 609, 615
Ppu21I YACGTR 1 cut(s) 402
Psp6I CCWGG 1 cut(s) 513
PspFI CCCAGC 1 cut(s) 923
PspGI CCWGG 1 cut(s) 513
PspN4I GGNNCC 4 cut(s) 179, 512, 539, 775
PspPI GGNCC 2 cut(s) 467, 1006
PspXI VCTCGAGB 1 cut(s) 623
PsyI GACNNNGTC 1 cut(s) 733
PvuI CGATCG 1 cut(s) 508
PvuII CAGCTG 2 cut(s) 114, 1088
RigI GGCCGGCC 1 cut(s) 174
RsaI GTAC 4 cut(s) 5, 151, 179, 356
RsaNI GTAC 4 cut(s) 4, 150, 178, 355
RseI CAYNNNNRTG 2 cut(s) 350, 551
SaqAI TTAA 4 cut(s) 336, 1107, 1113, 1122
SatI GCNGC 7 cut(s) 112, 115, 168, 251, 828, 979, 1086
Sau3AI GATC 3 cut(s) 448, 505, 792
Sau96I GGNCC 2 cut(s) 467, 1006
SchI GAGTC 4 cut(s) 34, 579, 609, 615
ScrFI CCNGG 2 cut(s) 515, 751
SduI GDGCHC 2 cut(s) 104, 504
SexAI ACCWGGT 1 cut(s) 513
SfaNI GCATC 1 cut(s) 755
SfoI GGCGCC 1 cut(s) 539
Sfr274I CTCGAG 1 cut(s) 623
SfuI TTCGAA 1 cut(s) 438
SinI GGWCC 2 cut(s) 467, 1006
SlaI CTCGAG 1 cut(s) 623
SmiMI CAYNNNNRTG 2 cut(s) 350, 551
SmlI CTYRAG 1 cut(s) 623
SmoI CTYRAG 1 cut(s) 623
Sse9I AATT 6 cut(s) 13, 373, 756, 895, 1071, 1119
SsiI CCGC 9 cut(s) 167, 218, 248, 251, 255, 275, 474, 827, 1049
SspDI GGCGCC 1 cut(s) 537
SspI AATATT 1 cut(s) 1040
SspMI CTAG 4 cut(s) 384, 549, 1046, 1055
StyD4I CCNGG 2 cut(s) 513, 749
StyI CCWWGG 1 cut(s) 871
TaaI ACNGT 3 cut(s) 22, 733, 768
TaiI ACGT 1 cut(s) 404
TaqI TCGA 5 cut(s) 438, 447, 504, 624, 728
TaqII GACCGA 1 cut(s) 1085
TasI AATT 6 cut(s) 13, 373, 756, 895, 1071, 1119
TatI WGTACW 1 cut(s) 3
TauI GCSGC 3 cut(s) 170, 253, 830
TfiI GAWTC 2 cut(s) 648, 1139
Tru1I TTAA 4 cut(s) 336, 1107, 1113, 1122
Tru9I TTAA 4 cut(s) 336, 1107, 1113, 1122
TscAI CASTG 1 cut(s) 150
TseI GCWGC 4 cut(s) 111, 114, 978, 1085
TspDTI ATGAA 4 cut(s) 446, 551, 566, 940
TspGWI ACGGA 2 cut(s) 297, 1132
TspRI CASTG 1 cut(s) 150
Tth111I GACNNNGTC 1 cut(s) 733
VneI GTGCAC 1 cut(s) 100
VpaK11BI GGWCC 2 cut(s) 467, 1006
XbaI TCTAGA 1 cut(s) 383
XceI RCATGY 2 cut(s) 349, 556
XhoI CTCGAG 1 cut(s) 623
XspI CTAG 4 cut(s) 384, 549, 1046, 1055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.