Prupe.3G083700_v2.0.a1

Rhomboid-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0016892
Physical Location & Seq
Reverse (-)
6187175 .. 6190454
3280 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G083700.1

Sequence Viewer

Length: 951 bp
ATGTATCCACCACCACCCGCGCCATACTATGCTCCACCTCCACCAGCGTATTACGCGCCGCCACTAATGCCGCCACCACCAAAGCGGTACTCTCCGTGGCTGGTGCCGCTTATCTTTTTGGTGAATCTCGGGTTGTTCATATGGATCATGTATGAGAATAATTGCCCGTCTAGAATTTCAAAGGATCAATGCATGTTAGGCCAGTATTTGGATAGGTTCTCTTTCCAACCCTGGAAAGATAACCGCATGGTTGGTCCTACCCCTGAAACACTTCAAAGACTAGGAGGCCTTGATAGAAAACTAGTGGTGGATGGTGGTGAACCATGGCGCCTTTTGTCCTCCATGTGGCTACATGCCGGGCTGATTCATCTGTTTGCCAACATGCTCAGCCTTGATTTTGTAGGACTTCGGCTTGAGCGGGACTTTGGATTTTACAGATTCGTATTCGTGTATGTGCTAGCTGGGTTAGGTGGAAATTTAGCGTCTTGCCTTAATATTATCAAACACGACTGGTCAAGCAAGACTATATCAGTTGGCGCATCTGGGGCAATTTTTGGATTGTTGGGAGCTTCCCTTTCTGAGCTCATCACAAACTGGACAGTCTATGACGATAAGTGCTCAACAATCATGATACTCATCCTCAATGCTGCCCTGAACTTGGCTATTGGGTTTCTACTTAAGATGGACAACTCAGGTCATGTAGGAGGACTCGTCGCCGGATTTTTCCTCGGTTTTGTTCTTTTTGTCAAGCCTCAGTTTGGATATGTAAGCAGCAAGTACATTCCATCATACCATCAAGTCAAACGTACACCAAGGCACAACTTCTGTCAATATTTTCTAGGGCTCGTTGCTTTGGCCGTGTTGGTTATTCTATATGGAGTTGGCTTTGGTAAGCTATTCAACATAAAGGAGATTAAAGAGCATCTGCCTGACAGCGTAAAAAACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

317

Amino Acids

35.51

Weight (kDa)

9.16

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016892)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g29980
malus_domestica MD00G1022400.v1.1 MD00G1143800.v1.1
prunus_persica Prupe.3G083700_v2.0.a1
pyrus_communis pycom17g21420
rosa_chinensis RchiOBHm_Chr2g0135991
rosa_laevigata RLG00000019495
rosa_roxburghii Rroxscaffold_2G00108710
rosa_rugosa Rorug02G0327200
rosa_samantha Rh2AG379200 Rh2BG385700 Rh2CG364800 Rh2DG402000
rosa_wichuraiana Rw2G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 103, 327
AccB7I CCANNNNNTGG 1 cut(s) 208
AccBSI CCGCTC 1 cut(s) 418
AccII CGCG 2 cut(s) 20, 56
AciI CCGC 7 cut(s) 18, 59, 71, 85, 107, 244, 418
AclWI GGATC 2 cut(s) 152, 192
AcoI YGGCCR 1 cut(s) 855
AcsI RAATTY 2 cut(s) 174, 475
AcyI GRCGYC 1 cut(s) 328
AfaI GTAC 3 cut(s) 89, 779, 808
AfiI CCNNNNNNNGG 4 cut(s) 208, 345, 658, 758
AflII CTTAAG 1 cut(s) 677
AgsI TTSAA 3 cut(s) 180, 275, 901
AhlI ACTAGT 1 cut(s) 301
AjnI CCWGG 1 cut(s) 230
AluBI AGCT 4 cut(s) 461, 569, 583, 895
AluI AGCT 4 cut(s) 461, 569, 583, 895
Alw21I GWGCWC 2 cut(s) 585, 620
AlwI GGATC 2 cut(s) 152, 192
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 3 cut(s) 199, 286, 855
ApeKI GCWGC 2 cut(s) 647, 771
ApoI RAATTY 2 cut(s) 174, 475
Asp700I GAANNNNTTC 1 cut(s) 270
AspLEI GCGC 4 cut(s) 22, 58, 330, 539
AspS9I GGNCC 1 cut(s) 254
AsuC2I CCSGG 1 cut(s) 358
AsuHPI GGTGA 2 cut(s) 133, 329
AsuNHI GCTAGC 1 cut(s) 457
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 1 cut(s) 254
BanI GGYRCC 2 cut(s) 103, 327
BanII GRGCYC 2 cut(s) 585, 846
Bbv12I GWGCWC 2 cut(s) 585, 620
BbvI GCAGC 2 cut(s) 634, 783
BccI CCATC 4 cut(s) 305, 676, 793, 801
BceAI ACGGC 1 cut(s) 842
BciT130I CCWGG 1 cut(s) 232
BciVI GTATCC 1 cut(s) 15
BcnI CCSGG 1 cut(s) 358
BcuI ACTAGT 1 cut(s) 301
BfaI CTAG 5 cut(s) 171, 281, 302, 458, 839
BfoI RGCGCY 1 cut(s) 331
BfrI CTTAAG 1 cut(s) 677
BfuI GTATCC 1 cut(s) 15
BisI GCNGC 5 cut(s) 59, 71, 107, 648, 772
BlpI GCTNAGC 1 cut(s) 386
BlsI GCNGC 5 cut(s) 60, 72, 108, 649, 773
Bme1390I CCNGG 2 cut(s) 232, 358
Bme18I GGWCC 1 cut(s) 254
BmeT110I CYCGRG 1 cut(s) 128
BmgT120I GGNCC 1 cut(s) 254
BmiI GGNNCC 2 cut(s) 105, 329
BmrFI CCNGG 2 cut(s) 232, 358
BmsI GCATC 2 cut(s) 548, 931
BmtI GCTAGC 1 cut(s) 461
Bpu1102I GCTNAGC 1 cut(s) 386
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 1 cut(s) 358
BsaBI GATNNNNATC 1 cut(s) 635
BsaHI GRCGYC 1 cut(s) 328
BsaJI CCNNGG 5 cut(s) 95, 230, 323, 727, 812
BsaXI ACNNNNNCTCC 2 cut(s) 696, 726
Bsc4I CCNNNNNNNGG 4 cut(s) 208, 345, 658, 758
Bse1I ACTGG 3 cut(s) 202, 515, 599
Bse8I GATNNNNATC 1 cut(s) 635
BseBI CCWGG 1 cut(s) 232
BseDI CCNNGG 5 cut(s) 95, 230, 323, 727, 812
BseGI GGATG 2 cut(s) 316, 636
BseJI GATNNNNATC 1 cut(s) 635
BseLI CCNNNNNNNGG 4 cut(s) 208, 345, 658, 758
BseMII CTCAG 4 cut(s) 400, 570, 705, 767
BseNI ACTGG 3 cut(s) 202, 515, 599
BseXI GCAGC 2 cut(s) 634, 783
BseYI CCCAGC 1 cut(s) 461
Bsh1236I CGCG 2 cut(s) 20, 56
BshFI GGCC 3 cut(s) 201, 288, 857
BshNI GGYRCC 2 cut(s) 103, 327
BsiHKAI GWGCWC 2 cut(s) 585, 620
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 2 cut(s) 357, 717
BslFI GGGAC 1 cut(s) 434
BslI CCNNNNNNNGG 4 cut(s) 208, 345, 658, 758
BsmFI GGGAC 1 cut(s) 434
BsnI GGCC 3 cut(s) 201, 288, 857
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 3 cut(s) 585, 620, 846
Bsp143I GATC 2 cut(s) 144, 184
Bsp1720I GCTNAGC 1 cut(s) 386
Bsp19I CCATGG 1 cut(s) 323
BspACI CCGC 7 cut(s) 18, 59, 71, 85, 107, 244, 418
BspANI GGCC 3 cut(s) 201, 288, 857
BspCNI CTCAG 4 cut(s) 399, 571, 704, 766
BspFNI CGCG 2 cut(s) 20, 56
BspHI TCATGA 1 cut(s) 627
BspLI GGNNCC 2 cut(s) 105, 329
BspOI GCTAGC 1 cut(s) 461
BspPI GGATC 2 cut(s) 152, 192
BspT107I GGYRCC 2 cut(s) 103, 327
BspTI CTTAAG 1 cut(s) 677
BsrBI CCGCTC 1 cut(s) 418
BsrI ACTGG 3 cut(s) 202, 515, 599
BssECI CCNNGG 5 cut(s) 95, 230, 323, 727, 812
BssMI GATC 2 cut(s) 144, 184
BssNI GRCGYC 1 cut(s) 328
BssT1I CCWWGG 2 cut(s) 323, 812
Bst2UI CCWGG 1 cut(s) 232
Bst4CI ACNGT 1 cut(s) 601
BstACI GRCGYC 1 cut(s) 328
BstAFI CTTAAG 1 cut(s) 677
BstC8I GCNNGC 1 cut(s) 459
BstDEI CTNAG 4 cut(s) 386, 579, 691, 753
BstDSI CCRYGG 2 cut(s) 95, 323
BstF5I GGATG 2 cut(s) 316, 636
BstFNI CGCG 2 cut(s) 20, 56
BstH2I RGCGCY 1 cut(s) 331
BstHHI GCGC 4 cut(s) 22, 58, 330, 539
BstKTI GATC 2 cut(s) 147, 187
BstMBI GATC 2 cut(s) 144, 184
BstMWI GCNNNNNNNGC 5 cut(s) 53, 67, 106, 198, 545
BstNI CCWGG 1 cut(s) 232
BstNSI RCATGY 3 cut(s) 196, 356, 385
BstSCI CCNGG 2 cut(s) 230, 356
BstUI CGCG 2 cut(s) 20, 56
BstV1I GCAGC 2 cut(s) 634, 783
BsuI GTATCC 1 cut(s) 15
BsuRI GGCC 3 cut(s) 201, 288, 857
BtgI CCRYGG 2 cut(s) 95, 323
BtsCI GGATG 2 cut(s) 316, 636
Cac8I GCNNGC 1 cut(s) 459
CciI TCATGA 1 cut(s) 627
CfoI GCGC 4 cut(s) 22, 58, 330, 539
Cfr13I GGNCC 1 cut(s) 254
CseI GACGC 1 cut(s) 471
Csp6I GTAC 3 cut(s) 88, 778, 807
CviAII CATG 9 cut(s) 148, 193, 247, 324, 343, 353, 382, 628, 698
CviQI GTAC 3 cut(s) 88, 778, 807
DdeI CTNAG 4 cut(s) 386, 579, 691, 753
DinI GGCGCC 1 cut(s) 329
DpnI GATC 2 cut(s) 146, 186
DpnII GATC 2 cut(s) 144, 184
EaeI YGGCCR 1 cut(s) 855
Ecl136II GAGCTC 1 cut(s) 583
Eco130I CCWWGG 2 cut(s) 323, 812
Eco147I AGGCCT 1 cut(s) 288
Eco24I GRGCYC 2 cut(s) 585, 846
Eco47I GGWCC 1 cut(s) 254
Eco53kI GAGCTC 1 cut(s) 583
Eco88I CYCGRG 1 cut(s) 128
EcoICRI GAGCTC 1 cut(s) 583
EcoRII CCWGG 1 cut(s) 230
EcoT14I CCWWGG 2 cut(s) 323, 812
EcoT22I ATGCAT 1 cut(s) 194
EcoT38I GRGCYC 2 cut(s) 585, 846
EgeI GGCGCC 1 cut(s) 329
EheI GGCGCC 1 cut(s) 329
ErhI CCWWGG 2 cut(s) 323, 812
FaeI CATG 9 cut(s) 151, 196, 250, 327, 346, 356, 385, 631, 701
FaqI GGGAC 1 cut(s) 434
FatI CATG 9 cut(s) 147, 192, 246, 323, 342, 352, 381, 627, 697
FauI CCCGC 2 cut(s) 25, 411
FauNDI CATATG 1 cut(s) 140
Fnu4HI GCNGC 5 cut(s) 59, 71, 107, 648, 772
FokI GGATG 2 cut(s) 323, 623
FriOI GRGCYC 2 cut(s) 585, 846
Fsp4HI GCNGC 5 cut(s) 59, 71, 107, 648, 772
FspBI CTAG 5 cut(s) 171, 281, 302, 458, 839
GlaI GCGC 4 cut(s) 21, 57, 329, 538
GluI GCNGC 5 cut(s) 59, 71, 107, 648, 772
GsaI CCCAGC 1 cut(s) 465
HaeII RGCGCY 1 cut(s) 331
HaeIII GGCC 3 cut(s) 201, 288, 857
HapII CCGG 2 cut(s) 357, 717
HgaI GACGC 1 cut(s) 471
HhaI GCGC 4 cut(s) 22, 58, 330, 539
Hin1I GRCGYC 1 cut(s) 328
Hin1II CATG 9 cut(s) 151, 196, 250, 327, 346, 356, 385, 631, 701
Hin6I GCGC 4 cut(s) 20, 56, 328, 537
HinP1I GCGC 4 cut(s) 20, 56, 328, 537
HinfI GANTC 4 cut(s) 124, 364, 438, 708
HpaII CCGG 2 cut(s) 357, 717
HphI GGTGA 2 cut(s) 133, 329
Hpy166II GTNNAC 2 cut(s) 320, 809
Hpy188I TCNGA 1 cut(s) 580
Hpy188III TCNNGA 2 cut(s) 171, 628
Hpy8I GTNNAC 2 cut(s) 320, 809
Hpy99I CGWCG 1 cut(s) 716
HpyCH4III ACNGT 1 cut(s) 601
HpyCH4IV ACGT 1 cut(s) 805
HpyCH4V TGCA 1 cut(s) 192
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 67, 106, 198, 545
HpyF3I CTNAG 4 cut(s) 386, 579, 691, 753
HpySE526I ACGT 1 cut(s) 805
Hsp92I GRCGYC 1 cut(s) 328
Hsp92II CATG 9 cut(s) 151, 196, 250, 327, 346, 356, 385, 631, 701
HspAI GCGC 4 cut(s) 20, 56, 328, 537
KasI GGCGCC 1 cut(s) 327
Kzo9I GATC 2 cut(s) 144, 184
LmnI GCTCC 2 cut(s) 37, 566
Lsp1109I GCAGC 2 cut(s) 634, 783
LweI GCATC 2 cut(s) 548, 931
MaeI CTAG 5 cut(s) 171, 281, 302, 458, 839
MaeII ACGT 1 cut(s) 805
MalI GATC 2 cut(s) 146, 186
MbiI CCGCTC 1 cut(s) 418
MboI GATC 2 cut(s) 144, 184
MhlI GDGCHC 3 cut(s) 585, 620, 846
MluCI AATT 4 cut(s) 160, 174, 475, 549
Mly113I GGCGCC 1 cut(s) 328
MlyI GAGTC 1 cut(s) 702
MmeI TCCRAC 1 cut(s) 250
MnlI CCTC 7 cut(s) 48, 278, 349, 650, 698, 737, 762
Mph1103I ATGCAT 1 cut(s) 194
MroXI GAANNNNTTC 1 cut(s) 270
MseI TTAA 3 cut(s) 492, 678, 915
MspCI CTTAAG 1 cut(s) 677
MspI CCGG 2 cut(s) 357, 717
MspR9I CCNGG 2 cut(s) 232, 358
MvaI CCWGG 1 cut(s) 232
MvnI CGCG 2 cut(s) 20, 56
MwoI GCNNNNNNNGC 5 cut(s) 53, 67, 106, 198, 545
NarI GGCGCC 1 cut(s) 328
NciI CCSGG 1 cut(s) 358
NcoI CCATGG 1 cut(s) 323
NdeI CATATG 1 cut(s) 140
NdeII GATC 2 cut(s) 144, 184
NheI GCTAGC 1 cut(s) 457
NlaIII CATG 9 cut(s) 151, 196, 250, 327, 346, 356, 385, 631, 701
NlaIV GGNNCC 2 cut(s) 105, 329
NsiI ATGCAT 1 cut(s) 194
NspI RCATGY 3 cut(s) 196, 356, 385
PagI TCATGA 1 cut(s) 627
PceI AGGCCT 1 cut(s) 288
PdmI GAANNNNTTC 1 cut(s) 270
PfeI GAWTC 3 cut(s) 124, 364, 438
PflMI CCANNNNNTGG 1 cut(s) 208
PkrI GCNGC 5 cut(s) 60, 72, 108, 649, 773
PleI GAGTC 1 cut(s) 702
PluTI GGCGCC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 702
Psp124BI GAGCTC 1 cut(s) 585
Psp6I CCWGG 1 cut(s) 230
PspFI CCCAGC 1 cut(s) 461
PspGI CCWGG 1 cut(s) 230
PspN4I GGNNCC 2 cut(s) 105, 329
PspPI GGNCC 1 cut(s) 254
RsaI GTAC 3 cut(s) 89, 779, 808
RsaNI GTAC 3 cut(s) 88, 778, 807
SacI GAGCTC 1 cut(s) 585
SaqAI TTAA 3 cut(s) 492, 678, 915
SatI GCNGC 5 cut(s) 59, 71, 107, 648, 772
Sau3AI GATC 2 cut(s) 144, 184
Sau96I GGNCC 1 cut(s) 254
SchI GAGTC 1 cut(s) 702
ScrFI CCNGG 2 cut(s) 232, 358
SduI GDGCHC 3 cut(s) 585, 620, 846
SetI ASST 9 cut(s) 40, 218, 463, 472, 571, 585, 697, 808, 897
SfaNI GCATC 2 cut(s) 548, 931
SfoI GGCGCC 1 cut(s) 329
SinI GGWCC 1 cut(s) 254
SmlI CTYRAG 2 cut(s) 413, 677
SmoI CTYRAG 2 cut(s) 413, 677
SpeI ACTAGT 1 cut(s) 301
Sse9I AATT 4 cut(s) 160, 174, 475, 549
SseBI AGGCCT 1 cut(s) 288
SsiI CCGC 7 cut(s) 18, 59, 71, 85, 107, 244, 418
SspDI GGCGCC 1 cut(s) 327
SspI AATATT 2 cut(s) 496, 833
SspMI CTAG 5 cut(s) 171, 281, 302, 458, 839
SstI GAGCTC 1 cut(s) 585
StuI AGGCCT 1 cut(s) 288
StyD4I CCNGG 2 cut(s) 230, 356
StyI CCWWGG 2 cut(s) 323, 812
TaaI ACNGT 1 cut(s) 601
TaiI ACGT 1 cut(s) 808
TasI AATT 4 cut(s) 160, 174, 475, 549
TatI WGTACW 1 cut(s) 777
TauI GCSGC 3 cut(s) 61, 73, 109
TfiI GAWTC 3 cut(s) 124, 364, 438
Tru1I TTAA 3 cut(s) 492, 678, 915
Tru9I TTAA 3 cut(s) 492, 678, 915
TseI GCWGC 2 cut(s) 647, 771
TspDTI ATGAA 2 cut(s) 127, 356
TspGWI ACGGA 1 cut(s) 84
Van91I CCANNNNNTGG 1 cut(s) 208
Vha464I CTTAAG 1 cut(s) 677
VpaK11BI GGWCC 1 cut(s) 254
XapI RAATTY 2 cut(s) 174, 475
XbaI TCTAGA 1 cut(s) 170
XceI RCATGY 3 cut(s) 196, 356, 385
XmnI GAANNNNTTC 1 cut(s) 270
XspI CTAG 5 cut(s) 171, 281, 302, 458, 839
Zsp2I ATGCAT 1 cut(s) 194
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.