MD00G1022400.v1.1

Rhomboid-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0016892
Physical Location & Seq
Reverse (-)
3829798 .. 3832220
2423 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1022400.v1.1.491

Sequence Viewer

Length: 948 bp
ATGTATCCACCTCCGCCGCCATACTATGGCGCTCCACCTCCAGCAGCCTTCTACGCGCCACCACCAATGATGGCACCACCAAGGCGGTACTCTCCATGGCTGGTGCCGCTTATATTCTTGGTGAATCTCGGGATTTTCATATGGATAATGTATGAGAACAACTGCCCCTCTAGAATGTCAAAAGATCAGTGTATGGGAGGGCATTTTTTGGATAGATTCTCATTCCAACCTTGGCGCGATAACCGCATGATTGGTCCTACCCCTGAAACTCTCCAAAGACTAGGAGGCCTTGATAATAAACTAGTGCTGAATGGTGAAGCATGGCGCCTGTTCTCCGCAATGTGGCTACATGCTGGGCTGATTCATCTGTTCGTCAACATGCTCAGTCTTGATTTCGTAGGGCTCCGGCTTGAGCAGGATTTTGGATTTCACAGATTCGGATTCGTGTATGTGCTAGCTGGGTTAGGGGGAAGTTTAGGGTCTTGCCTTAATATTATCAAACAACAAAGGACAAGCAAGACTATATCAGTTGGCGCATCTGGGGCAATTTTTGGATTGTTGGGAGCCTCACTTTCTGAGCTGATCACAAACTGGACAGTCTATGACGATAAGTGCTCGACAATCATGATACTCATCCTCAATGCTGCCCTGAACTTGCTCATTGGATTTCTACTTCATATGGACAATTCAGCTCATGTAGGAGGACTCGTCGCTGGATTTTTTCTTGGTTTTGTGTTTTTTGTTAAGCCTCAGTTGGGATATGTAAGCAGCAAATACATGCCAACACACACTCAAGTCAAGCGTACAGCAAGGCACAACTTCTGTCAATATTTTCTGGGGGTCGTTGGTTTGGTCGTGTCCATTATTCTATTTGCAACTGCCTTCGGTAAGCTATTTAACATAAAGGAGATTAAACAGCATCTGCCTGATAGTGTAAACAAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

316

Amino Acids

35.26

Weight (kDa)

9.36

Isoelectric Point (pI)

44.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 105 - 247 3.2e-37 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016892)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g29980
malus_domestica MD00G1022400.v1.1 MD00G1143800.v1.1
prunus_persica Prupe.3G083700_v2.0.a1
pyrus_communis pycom17g21420
rosa_chinensis RchiOBHm_Chr2g0135991
rosa_laevigata RLG00000019495
rosa_roxburghii Rroxscaffold_2G00108710
rosa_rugosa Rorug02G0327200
rosa_samantha Rh2AG379200 Rh2BG385700 Rh2CG364800 Rh2DG402000
rosa_wichuraiana Rw2G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 73, 103, 324
AccB7I CCANNNNNTGG 1 cut(s) 26
AccII CGCG 2 cut(s) 56, 237
AciI CCGC 6 cut(s) 14, 17, 85, 107, 244, 336
AcyI GRCGYC 1 cut(s) 325
AfaI GTAC 2 cut(s) 89, 805
AfiI CCNNNNNNNGG 3 cut(s) 26, 342, 755
AhlI ACTAGT 1 cut(s) 301
AluBI AGCT 4 cut(s) 458, 580, 692, 892
AluI AGCT 4 cut(s) 458, 580, 692, 892
Alw21I GWGCWC 1 cut(s) 617
AlwNI CAGNNNCTG 1 cut(s) 922
Ama87I CYCGRG 1 cut(s) 128
AoxI GGCC 1 cut(s) 286
ApeKI GCWGC 3 cut(s) 44, 644, 768
AspLEI GCGC 5 cut(s) 32, 58, 237, 327, 536
AspS9I GGNCC 1 cut(s) 254
AsuHPI GGTGA 2 cut(s) 133, 326
AsuNHI GCTAGC 1 cut(s) 454
AvaI CYCGRG 1 cut(s) 128
AvaII GGWCC 1 cut(s) 254
BanI GGYRCC 3 cut(s) 73, 103, 324
BanII GRGCYC 1 cut(s) 405
Bbv12I GWGCWC 1 cut(s) 617
BbvI GCAGC 3 cut(s) 56, 631, 780
BccI CCATC 1 cut(s) 64
BciVI GTATCC 1 cut(s) 15
BclI TGATCA 1 cut(s) 582
BcuI ACTAGT 1 cut(s) 301
BfaI CTAG 4 cut(s) 171, 281, 302, 455
BfoI RGCGCY 2 cut(s) 33, 328
BfuI GTATCC 1 cut(s) 15
BisI GCNGC 5 cut(s) 17, 45, 107, 645, 769
BlsI GCNGC 5 cut(s) 18, 46, 108, 646, 770
Bme18I GGWCC 1 cut(s) 254
BmeT110I CYCGRG 1 cut(s) 128
BmgT120I GGNCC 1 cut(s) 254
BmiI GGNNCC 5 cut(s) 75, 105, 326, 404, 565
BmsI GCATC 2 cut(s) 545, 928
BmtI GCTAGC 1 cut(s) 458
BpmI CTGGAG 1 cut(s) 24
BpuEI CTTGAG 2 cut(s) 431, 777
BsaBI GATNNNNATC 1 cut(s) 632
BsaHI GRCGYC 1 cut(s) 325
BsaJI CCNNGG 3 cut(s) 80, 95, 230
BsaXI ACNNNNNCTCC 2 cut(s) 693, 723
Bsc4I CCNNNNNNNGG 3 cut(s) 26, 342, 755
Bse1I ACTGG 1 cut(s) 596
Bse3DI GCAATG 1 cut(s) 345
Bse8I GATNNNNATC 1 cut(s) 632
BseDI CCNNGG 3 cut(s) 80, 95, 230
BseGI GGATG 1 cut(s) 633
BseJI GATNNNNATC 1 cut(s) 632
BseLI CCNNNNNNNGG 3 cut(s) 26, 342, 755
BseMI GCAATG 1 cut(s) 345
BseMII CTCAG 3 cut(s) 397, 567, 764
BseNI ACTGG 1 cut(s) 596
BseXI GCAGC 3 cut(s) 56, 631, 780
BseYI CCCAGC 2 cut(s) 353, 458
Bsh1236I CGCG 2 cut(s) 56, 237
BshFI GGCC 1 cut(s) 288
BshNI GGYRCC 3 cut(s) 73, 103, 324
BsiHKAI GWGCWC 1 cut(s) 617
BsiHKCI CYCGRG 1 cut(s) 128
BsiSI CCGG 1 cut(s) 406
BslI CCNNNNNNNGG 3 cut(s) 26, 342, 755
BsnI GGCC 1 cut(s) 288
BsoBI CYCGRG 1 cut(s) 128
Bsp1286I GDGCHC 2 cut(s) 405, 617
Bsp143I GATC 2 cut(s) 184, 582
Bsp19I CCATGG 1 cut(s) 95
BspACI CCGC 6 cut(s) 14, 17, 85, 107, 244, 336
BspANI GGCC 1 cut(s) 288
BspCNI CTCAG 3 cut(s) 396, 568, 763
BspFNI CGCG 2 cut(s) 56, 237
BspHI TCATGA 1 cut(s) 624
BspLI GGNNCC 5 cut(s) 75, 105, 326, 404, 565
BspOI GCTAGC 1 cut(s) 458
BspT107I GGYRCC 3 cut(s) 73, 103, 324
BsrDI GCAATG 1 cut(s) 345
BsrI ACTGG 1 cut(s) 596
BssECI CCNNGG 3 cut(s) 80, 95, 230
BssMI GATC 2 cut(s) 184, 582
BssNI GRCGYC 1 cut(s) 325
BssT1I CCWWGG 3 cut(s) 80, 95, 230
Bst4CI ACNGT 1 cut(s) 598
BstACI GRCGYC 1 cut(s) 325
BstC8I GCNNGC 1 cut(s) 456
BstDEI CTNAG 3 cut(s) 383, 576, 750
BstDSI CCRYGG 1 cut(s) 95
BstF5I GGATG 1 cut(s) 633
BstFNI CGCG 2 cut(s) 56, 237
BstH2I RGCGCY 2 cut(s) 33, 328
BstHHI GCGC 5 cut(s) 32, 58, 237, 327, 536
BstKTI GATC 2 cut(s) 187, 585
BstMBI GATC 2 cut(s) 184, 582
BstMWI GCNNNNNNNGC 4 cut(s) 53, 106, 243, 542
BstNSI RCATGY 3 cut(s) 353, 382, 781
BstUI CGCG 2 cut(s) 56, 237
BstV1I GCAGC 3 cut(s) 56, 631, 780
BsuI GTATCC 1 cut(s) 15
BsuRI GGCC 1 cut(s) 288
BtgI CCRYGG 1 cut(s) 95
BtsCI GGATG 1 cut(s) 633
BtsIMutI CAGTG 1 cut(s) 194
Cac8I GCNNGC 1 cut(s) 456
CaiI CAGNNNCTG 1 cut(s) 922
CciI TCATGA 1 cut(s) 624
CfoI GCGC 5 cut(s) 32, 58, 237, 327, 536
Cfr13I GGNCC 1 cut(s) 254
Csp6I GTAC 2 cut(s) 88, 804
CviAII CATG 8 cut(s) 96, 247, 321, 350, 379, 625, 695, 778
CviQI GTAC 2 cut(s) 88, 804
DdeI CTNAG 3 cut(s) 383, 576, 750
DinI GGCGCC 1 cut(s) 326
DpnI GATC 2 cut(s) 186, 584
DpnII GATC 2 cut(s) 184, 582
Eco130I CCWWGG 3 cut(s) 80, 95, 230
Eco147I AGGCCT 1 cut(s) 288
Eco24I GRGCYC 1 cut(s) 405
Eco47I GGWCC 1 cut(s) 254
Eco88I CYCGRG 1 cut(s) 128
EcoT14I CCWWGG 3 cut(s) 80, 95, 230
EcoT38I GRGCYC 1 cut(s) 405
EgeI GGCGCC 1 cut(s) 326
EheI GGCGCC 1 cut(s) 326
ErhI CCWWGG 3 cut(s) 80, 95, 230
FaeI CATG 8 cut(s) 99, 250, 324, 353, 382, 628, 698, 781
FatI CATG 8 cut(s) 95, 246, 320, 349, 378, 624, 694, 777
FauNDI CATATG 2 cut(s) 140, 678
FbaI TGATCA 1 cut(s) 582
Fnu4HI GCNGC 5 cut(s) 17, 45, 107, 645, 769
FokI GGATG 1 cut(s) 620
FriOI GRGCYC 1 cut(s) 405
Fsp4HI GCNGC 5 cut(s) 17, 45, 107, 645, 769
FspBI CTAG 4 cut(s) 171, 281, 302, 455
GlaI GCGC 5 cut(s) 31, 57, 236, 326, 535
GluI GCNGC 5 cut(s) 17, 45, 107, 645, 769
GsaI CCCAGC 2 cut(s) 357, 462
GsuI CTGGAG 1 cut(s) 24
HaeII RGCGCY 2 cut(s) 33, 328
HaeIII GGCC 1 cut(s) 288
HapII CCGG 1 cut(s) 406
HhaI GCGC 5 cut(s) 32, 58, 237, 327, 536
Hin1I GRCGYC 1 cut(s) 325
Hin1II CATG 8 cut(s) 99, 250, 324, 353, 382, 628, 698, 781
Hin6I GCGC 5 cut(s) 30, 56, 235, 325, 534
HinP1I GCGC 5 cut(s) 30, 56, 235, 325, 534
HincII GTYRAC 1 cut(s) 376
HindII GTYRAC 1 cut(s) 376
HinfI GANTC 6 cut(s) 124, 216, 361, 435, 441, 705
HpaII CCGG 1 cut(s) 406
HphI GGTGA 2 cut(s) 133, 326
Hpy166II GTNNAC 2 cut(s) 376, 937
Hpy188I TCNGA 2 cut(s) 440, 577
Hpy188III TCNNGA 4 cut(s) 130, 171, 389, 625
Hpy8I GTNNAC 2 cut(s) 376, 937
Hpy99I CGWCG 1 cut(s) 713
HpyAV CCTTC 2 cut(s) 58, 892
HpyCH4III ACNGT 1 cut(s) 598
HpyCH4V TGCA 1 cut(s) 875
HpyF10VI GCNNNNNNNGC 4 cut(s) 53, 106, 243, 542
HpyF3I CTNAG 3 cut(s) 383, 576, 750
Hsp92I GRCGYC 1 cut(s) 325
Hsp92II CATG 8 cut(s) 99, 250, 324, 353, 382, 628, 698, 781
HspAI GCGC 5 cut(s) 30, 56, 235, 325, 534
KasI GGCGCC 1 cut(s) 324
Ksp22I TGATCA 1 cut(s) 582
Kzo9I GATC 2 cut(s) 184, 582
LmnI GCTCC 3 cut(s) 37, 408, 563
Lsp1109I GCAGC 3 cut(s) 56, 631, 780
LweI GCATC 2 cut(s) 545, 928
MaeI CTAG 4 cut(s) 171, 281, 302, 455
MalI GATC 2 cut(s) 186, 584
MboI GATC 2 cut(s) 184, 582
MhlI GDGCHC 2 cut(s) 405, 617
MluCI AATT 2 cut(s) 546, 685
Mly113I GGCGCC 1 cut(s) 325
MlyI GAGTC 1 cut(s) 699
MmeI TCCRAC 1 cut(s) 250
MnlI CCTC 9 cut(s) 21, 48, 178, 191, 278, 577, 647, 695, 759
MseI TTAA 4 cut(s) 489, 744, 897, 912
MspI CCGG 1 cut(s) 406
MvnI CGCG 2 cut(s) 56, 237
MwoI GCNNNNNNNGC 4 cut(s) 53, 106, 243, 542
NarI GGCGCC 1 cut(s) 325
NcoI CCATGG 1 cut(s) 95
NdeI CATATG 2 cut(s) 140, 678
NdeII GATC 2 cut(s) 184, 582
NheI GCTAGC 1 cut(s) 454
NlaIII CATG 8 cut(s) 99, 250, 324, 353, 382, 628, 698, 781
NlaIV GGNNCC 5 cut(s) 75, 105, 326, 404, 565
NspI RCATGY 3 cut(s) 353, 382, 781
PagI TCATGA 1 cut(s) 624
PceI AGGCCT 1 cut(s) 288
PfeI GAWTC 5 cut(s) 124, 216, 361, 435, 441
PflMI CCANNNNNTGG 1 cut(s) 26
PkrI GCNGC 5 cut(s) 18, 46, 108, 646, 770
PleI GAGTC 1 cut(s) 699
PluTI GGCGCC 1 cut(s) 328
PpsI GAGTC 1 cut(s) 699
PspFI CCCAGC 2 cut(s) 353, 458
PspN4I GGNNCC 5 cut(s) 75, 105, 326, 404, 565
PspPI GGNCC 1 cut(s) 254
PstNI CAGNNNCTG 1 cut(s) 922
RsaI GTAC 2 cut(s) 89, 805
RsaNI GTAC 2 cut(s) 88, 804
SaqAI TTAA 4 cut(s) 489, 744, 897, 912
SatI GCNGC 5 cut(s) 17, 45, 107, 645, 769
Sau3AI GATC 2 cut(s) 184, 582
Sau96I GGNCC 1 cut(s) 254
SchI GAGTC 1 cut(s) 699
SduI GDGCHC 2 cut(s) 405, 617
SetI ASST 7 cut(s) 13, 40, 232, 460, 582, 694, 894
SfaNI GCATC 2 cut(s) 545, 928
SfoI GGCGCC 1 cut(s) 326
SinI GGWCC 1 cut(s) 254
SmlI CTYRAG 2 cut(s) 410, 792
SmoI CTYRAG 2 cut(s) 410, 792
SpeI ACTAGT 1 cut(s) 301
Sse9I AATT 2 cut(s) 546, 685
SseBI AGGCCT 1 cut(s) 288
SsiI CCGC 6 cut(s) 14, 17, 85, 107, 244, 336
SspDI GGCGCC 1 cut(s) 324
SspI AATATT 3 cut(s) 493, 830, 944
SspMI CTAG 4 cut(s) 171, 281, 302, 455
StuI AGGCCT 1 cut(s) 288
StyI CCWWGG 3 cut(s) 80, 95, 230
TaaI ACNGT 1 cut(s) 598
TaqI TCGA 1 cut(s) 617
TasI AATT 2 cut(s) 546, 685
TauI GCSGC 2 cut(s) 19, 109
TfiI GAWTC 5 cut(s) 124, 216, 361, 435, 441
Tru1I TTAA 4 cut(s) 489, 744, 897, 912
Tru9I TTAA 4 cut(s) 489, 744, 897, 912
TscAI CASTG 1 cut(s) 194
TseI GCWGC 3 cut(s) 44, 644, 768
TspDTI ATGAA 3 cut(s) 127, 353, 665
TspRI CASTG 1 cut(s) 194
Van91I CCANNNNNTGG 1 cut(s) 26
VpaK11BI GGWCC 1 cut(s) 254
XbaI TCTAGA 1 cut(s) 170
XceI RCATGY 3 cut(s) 353, 382, 781
XspI CTAG 4 cut(s) 171, 281, 302, 455
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.