Rroxscaffold_2G00108710

Rhomboid-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016892
Physical Location & Seq
Reverse (-)
32778096 .. 32780108
2013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00108710.1

Sequence Viewer

Length: 954 bp
ATGTATTATCCACCAGGTCCGCCTCCACTGTATTATCCACCACCCCCGCCACAGTTTTACGCCCCACCGCCCTTGCCACCAGCCCCAAGGCGATACTCTCCGTGGCTTGTGCCTCTCATATGCTTGGTTAATCTTGGAATGTTTGTGTACGAAATGTATAAGAACAATTGCCCATCTAGACACCCAAGGGATCAGTGTATGTTAGGTGATCCTTTTGGCAGGTTCTCATTCGAACCCTTCAAAGAAGTCAAATGGATTGGTCCTTCCGCAGAAACTATTAAGAAATTAGGAGGCCTTGATCAGCAACTAGTTGTGAAAGGTGAATCATGGCGCCTTTTCTCTTGCATGTGGCTTCATGCCGGAGTCATTCATTTAATTTTCAACATACTCAGCCTTGATTTCGTTGGACTCCGACTTGAGCGCGACTTTGGATATCATAGGTTTGTGTTTGTGTATGTGCTTGCTGGGTTGGGCGGAAGTGTAGCTTCTTGTCTTCGTATTATTAAACAAGACTTGGCATCACATGCTGTGTCAGCTGGCGCATCTGGGGCAATTTTTGGATTGTGTGGAGCCTCCCTTTCTGAGCTGATCACAAACTGGACAATCTATGATGATAAGTGCTCGTCGATCATGATACTCGTTCTCAATCTTGTCTTGAACGTGGCCCTTGGATTTCTACTGAAAATGGACATGTCAGGTCATATAGGAGGTTTTGTAGCTGGGTTCTTCCTCGGTTTTGTTTTTTTCATCAAGCCTCAGCTTGGATATATAAGCAGCAAGTTTATTCCGGCATACCATGAAGTCAAACGTACACCAAGGCACAACTTCTGTCAATATTTCCTAGCGATTGTAGGTCTTGTCGGATCAATTGCTCTATTTGCGGCTGGCTTCGGTAAGCTATTTAACATTAAGGAAATTAAAGAGAAGTTGCCGAATCCCGTCAACAATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

317

Amino Acids

35.44

Weight (kDa)

9.11

Isoelectric Point (pI)

48.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 107 - 249 2.3e-36 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016892)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g29980
malus_domestica MD00G1022400.v1.1 MD00G1143800.v1.1
prunus_persica Prupe.3G083700_v2.0.a1
pyrus_communis pycom17g21420
rosa_chinensis RchiOBHm_Chr2g0135991
rosa_laevigata RLG00000019495
rosa_roxburghii Rroxscaffold_2G00108710
rosa_rugosa Rorug02G0327200
rosa_samantha Rh2AG379200 Rh2BG385700 Rh2CG364800 Rh2DG402000
rosa_wichuraiana Rw2G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 210
AccB1I GGYRCC 1 cut(s) 330
AccII CGCG 1 cut(s) 423
AciI CCGC 6 cut(s) 20, 47, 68, 267, 474, 881
AclWI GGATC 3 cut(s) 198, 203, 871
AcyI GRCGYC 1 cut(s) 331
AfaI GTAC 2 cut(s) 149, 811
AfiI CCNNNNNNNGG 1 cut(s) 761
AflIII ACRYGT 1 cut(s) 690
AgsI TTSAA 3 cut(s) 241, 382, 658
AhlI ACTAGT 1 cut(s) 307
AjnI CCWGG 1 cut(s) 13
AluBI AGCT 6 cut(s) 485, 536, 586, 719, 760, 898
AluI AGCT 6 cut(s) 485, 536, 586, 719, 760, 898
Alw21I GWGCWC 1 cut(s) 623
AlwI GGATC 3 cut(s) 198, 203, 871
AoxI GGCC 2 cut(s) 292, 663
ApeKI GCWGC 1 cut(s) 774
AspLEI GCGC 3 cut(s) 333, 423, 542
AspS9I GGNCC 3 cut(s) 17, 260, 664
AsuHPI GGTGA 2 cut(s) 218, 332
AsuII TTCGAA 1 cut(s) 231
AvaII GGWCC 2 cut(s) 17, 260
BanI GGYRCC 1 cut(s) 330
BbsI GAAGAC 1 cut(s) 485
Bbv12I GWGCWC 1 cut(s) 623
BbvCI CCTCAGC 1 cut(s) 756
BbvI GCAGC 1 cut(s) 786
BccI CCATC 1 cut(s) 181
BciT130I CCWGG 1 cut(s) 15
BclI TGATCA 2 cut(s) 298, 588
BcuI ACTAGT 1 cut(s) 307
BfaI CTAG 3 cut(s) 177, 308, 842
BfoI RGCGCY 1 cut(s) 334
BfuAI ACCTGC 1 cut(s) 210
BisI GCNGC 2 cut(s) 775, 882
BlsI GCNGC 2 cut(s) 776, 883
Bme1390I CCNGG 1 cut(s) 15
Bme18I GGWCC 2 cut(s) 17, 260
BmgT120I GGNCC 3 cut(s) 17, 260, 664
BmiI GGNNCC 2 cut(s) 332, 571
BmrFI CCNGG 1 cut(s) 15
BmsI GCATC 2 cut(s) 527, 551
BpiI GAAGAC 1 cut(s) 485
Bpu10I CCTNAGC 1 cut(s) 756
Bpu14I TTCGAA 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 437
BsaHI GRCGYC 1 cut(s) 331
BsaJI CCNNGG 6 cut(s) 86, 101, 185, 667, 730, 815
BsaXI ACNNNNNCTCC 2 cut(s) 699, 729
Bsc4I CCNNNNNNNGG 1 cut(s) 761
Bse1I ACTGG 1 cut(s) 602
BseBI CCWGG 1 cut(s) 15
BseDI CCNNGG 6 cut(s) 86, 101, 185, 667, 730, 815
BseLI CCNNNNNNNGG 1 cut(s) 761
BseMII CTCAG 3 cut(s) 403, 573, 770
BseNI ACTGG 1 cut(s) 602
BseXI GCAGC 1 cut(s) 786
BseYI CCCAGC 2 cut(s) 464, 719
Bsh1236I CGCG 1 cut(s) 423
BshFI GGCC 2 cut(s) 294, 665
BshNI GGYRCC 1 cut(s) 330
BsiHKAI GWGCWC 1 cut(s) 623
BsiSI CCGG 2 cut(s) 360, 788
BslI CCNNNNNNNGG 1 cut(s) 761
BsnI GGCC 2 cut(s) 294, 665
Bsp119I TTCGAA 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 623
Bsp143I GATC 6 cut(s) 190, 208, 298, 588, 627, 863
BspACI CCGC 6 cut(s) 20, 47, 68, 267, 474, 881
BspANI GGCC 2 cut(s) 294, 665
BspCNI CTCAG 3 cut(s) 402, 574, 769
BspFNI CGCG 1 cut(s) 423
BspHI TCATGA 1 cut(s) 630
BspLI GGNNCC 2 cut(s) 332, 571
BspMI ACCTGC 1 cut(s) 210
BspPI GGATC 3 cut(s) 198, 203, 871
BspT104I TTCGAA 1 cut(s) 231
BspT107I GGYRCC 1 cut(s) 330
BsrI ACTGG 1 cut(s) 602
BssECI CCNNGG 6 cut(s) 86, 101, 185, 667, 730, 815
BssMI GATC 6 cut(s) 190, 208, 298, 588, 627, 863
BssNI GRCGYC 1 cut(s) 331
BssT1I CCWWGG 4 cut(s) 86, 185, 667, 815
Bst2UI CCWGG 1 cut(s) 15
Bst4CI ACNGT 2 cut(s) 30, 54
BstACI GRCGYC 1 cut(s) 331
BstAPI GCANNNNNTGC 1 cut(s) 524
BstBI TTCGAA 1 cut(s) 231
BstC8I GCNNGC 3 cut(s) 462, 538, 886
BstDEI CTNAG 3 cut(s) 389, 582, 756
BstDSI CCRYGG 1 cut(s) 101
BstFNI CGCG 1 cut(s) 423
BstH2I RGCGCY 1 cut(s) 334
BstHHI GCGC 3 cut(s) 333, 423, 542
BstKTI GATC 6 cut(s) 193, 211, 301, 591, 630, 866
BstMBI GATC 6 cut(s) 190, 208, 298, 588, 627, 863
BstMWI GCNNNNNNNGC 4 cut(s) 524, 533, 548, 878
BstNI CCWGG 1 cut(s) 15
BstNSI RCATGY 3 cut(s) 349, 527, 694
BstSCI CCNGG 1 cut(s) 13
BstUI CGCG 1 cut(s) 423
BstV1I GCAGC 1 cut(s) 786
BstV2I GAAGAC 1 cut(s) 485
BsuRI GGCC 2 cut(s) 294, 665
BtgI CCRYGG 1 cut(s) 101
BtsIMutI CAGTG 2 cut(s) 26, 200
BveI ACCTGC 1 cut(s) 210
Cac8I GCNNGC 3 cut(s) 462, 538, 886
CciI TCATGA 1 cut(s) 630
CfoI GCGC 3 cut(s) 333, 423, 542
Cfr13I GGNCC 3 cut(s) 17, 260, 664
CsiI ACCWGGT 1 cut(s) 13
Csp6I GTAC 2 cut(s) 148, 810
CspCI CAANNNNNGTGG 2 cut(s) 54, 89
CviAII CATG 7 cut(s) 327, 346, 356, 524, 631, 691, 797
CviQI GTAC 2 cut(s) 148, 810
DdeI CTNAG 3 cut(s) 389, 582, 756
DinI GGCGCC 1 cut(s) 332
DpnI GATC 6 cut(s) 192, 210, 300, 590, 629, 865
DpnII GATC 6 cut(s) 190, 208, 298, 588, 627, 863
EciI GGCGGA 2 cut(s) 9, 489
Eco130I CCWWGG 4 cut(s) 86, 185, 667, 815
Eco147I AGGCCT 1 cut(s) 294
Eco32I GATATC 1 cut(s) 434
Eco47I GGWCC 2 cut(s) 17, 260
EcoRII CCWGG 1 cut(s) 13
EcoRV GATATC 1 cut(s) 434
EcoT14I CCWWGG 4 cut(s) 86, 185, 667, 815
EgeI GGCGCC 1 cut(s) 332
EheI GGCGCC 1 cut(s) 332
ErhI CCWWGG 4 cut(s) 86, 185, 667, 815
FaeI CATG 7 cut(s) 330, 349, 359, 527, 634, 694, 800
FalI AAGNNNNNCTT 2 cut(s) 469, 501
FatI CATG 7 cut(s) 326, 345, 355, 523, 630, 690, 796
FauI CCCGC 1 cut(s) 54
FauNDI CATATG 1 cut(s) 119
FbaI TGATCA 2 cut(s) 298, 588
Fnu4HI GCNGC 2 cut(s) 775, 882
Fsp4HI GCNGC 2 cut(s) 775, 882
FspBI CTAG 3 cut(s) 177, 308, 842
GlaI GCGC 3 cut(s) 332, 422, 541
GluI GCNGC 2 cut(s) 775, 882
GsaI CCCAGC 2 cut(s) 468, 723
HaeII RGCGCY 1 cut(s) 334
HaeIII GGCC 2 cut(s) 294, 665
HapII CCGG 2 cut(s) 360, 788
HhaI GCGC 3 cut(s) 333, 423, 542
Hin1I GRCGYC 1 cut(s) 331
Hin1II CATG 7 cut(s) 330, 349, 359, 527, 634, 694, 800
Hin6I GCGC 3 cut(s) 331, 421, 540
HinP1I GCGC 3 cut(s) 331, 421, 540
HincII GTYRAC 1 cut(s) 943
HindII GTYRAC 1 cut(s) 943
HinfI GANTC 4 cut(s) 323, 363, 408, 934
HpaII CCGG 2 cut(s) 360, 788
HphI GGTGA 2 cut(s) 218, 332
Hpy166II GTNNAC 3 cut(s) 148, 812, 943
Hpy188I TCNGA 3 cut(s) 413, 583, 863
Hpy188III TCNNGA 3 cut(s) 177, 631, 655
Hpy8I GTNNAC 3 cut(s) 148, 812, 943
Hpy99I CGWCG 1 cut(s) 628
HpyAV CCTTC 2 cut(s) 247, 273
HpyCH4III ACNGT 2 cut(s) 30, 54
HpyCH4IV ACGT 2 cut(s) 660, 808
HpyCH4V TGCA 1 cut(s) 345
HpyF10VI GCNNNNNNNGC 4 cut(s) 524, 533, 548, 878
HpyF3I CTNAG 3 cut(s) 389, 582, 756
HpySE526I ACGT 2 cut(s) 660, 808
Hsp92I GRCGYC 1 cut(s) 331
Hsp92II CATG 7 cut(s) 330, 349, 359, 527, 634, 694, 800
HspAI GCGC 3 cut(s) 331, 421, 540
KasI GGCGCC 1 cut(s) 330
Ksp22I TGATCA 2 cut(s) 298, 588
Kzo9I GATC 6 cut(s) 190, 208, 298, 588, 627, 863
LmnI GCTCC 1 cut(s) 569
Lsp1109I GCAGC 1 cut(s) 786
LweI GCATC 2 cut(s) 527, 551
MabI ACCWGGT 1 cut(s) 13
MaeI CTAG 3 cut(s) 177, 308, 842
MaeII ACGT 2 cut(s) 660, 808
MalI GATC 6 cut(s) 192, 210, 300, 590, 629, 865
MboI GATC 6 cut(s) 190, 208, 298, 588, 627, 863
MboII GAAGA 2 cut(s) 485, 718
MfeI CAATTG 2 cut(s) 166, 867
MhlI GDGCHC 1 cut(s) 623
MluCI AATT 7 cut(s) 166, 284, 375, 552, 867, 915, 946
Mly113I GGCGCC 1 cut(s) 331
MlyI GAGTC 2 cut(s) 372, 402
MmeI TCCRAC 3 cut(s) 385, 436, 841
MnlI CCTC 7 cut(s) 33, 123, 284, 583, 701, 740, 765
MseI TTAA 7 cut(s) 129, 279, 374, 504, 903, 909, 918
MspA1I CMGCKG 1 cut(s) 536
MspI CCGG 2 cut(s) 360, 788
MspR9I CCNGG 1 cut(s) 15
MunI CAATTG 2 cut(s) 166, 867
MvaI CCWGG 1 cut(s) 15
MvnI CGCG 1 cut(s) 423
MwoI GCNNNNNNNGC 4 cut(s) 524, 533, 548, 878
NarI GGCGCC 1 cut(s) 331
NdeI CATATG 1 cut(s) 119
NdeII GATC 6 cut(s) 190, 208, 298, 588, 627, 863
NlaIII CATG 7 cut(s) 330, 349, 359, 527, 634, 694, 800
NlaIV GGNNCC 2 cut(s) 332, 571
NspI RCATGY 3 cut(s) 349, 527, 694
NspV TTCGAA 1 cut(s) 231
PagI TCATGA 1 cut(s) 630
PceI AGGCCT 1 cut(s) 294
PciI ACATGT 1 cut(s) 690
PfeI GAWTC 2 cut(s) 323, 934
PkrI GCNGC 2 cut(s) 776, 883
PleI GAGTC 2 cut(s) 371, 402
PluTI GGCGCC 1 cut(s) 334
PpsI GAGTC 2 cut(s) 371, 402
PscI ACATGT 1 cut(s) 690
Psp6I CCWGG 1 cut(s) 13
PspFI CCCAGC 2 cut(s) 464, 719
PspGI CCWGG 1 cut(s) 13
PspN4I GGNNCC 2 cut(s) 332, 571
PspPI GGNCC 3 cut(s) 17, 260, 664
PvuII CAGCTG 1 cut(s) 536
RsaI GTAC 2 cut(s) 149, 811
RsaNI GTAC 2 cut(s) 148, 810
SaqAI TTAA 7 cut(s) 129, 279, 374, 504, 903, 909, 918
SatI GCNGC 2 cut(s) 775, 882
Sau3AI GATC 6 cut(s) 190, 208, 298, 588, 627, 863
Sau96I GGNCC 3 cut(s) 17, 260, 664
SchI GAGTC 2 cut(s) 372, 402
ScrFI CCNGG 1 cut(s) 15
SduI GDGCHC 1 cut(s) 623
SexAI ACCWGGT 1 cut(s) 13
SfaNI GCATC 2 cut(s) 527, 551
SfoI GGCGCC 1 cut(s) 332
SfuI TTCGAA 1 cut(s) 231
SinI GGWCC 2 cut(s) 17, 260
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
SpeI ACTAGT 1 cut(s) 307
Sse9I AATT 7 cut(s) 166, 284, 375, 552, 867, 915, 946
SseBI AGGCCT 1 cut(s) 294
SsiI CCGC 6 cut(s) 20, 47, 68, 267, 474, 881
SspDI GGCGCC 1 cut(s) 330
SspI AATATT 1 cut(s) 836
SspMI CTAG 3 cut(s) 177, 308, 842
StuI AGGCCT 1 cut(s) 294
StyD4I CCNGG 1 cut(s) 13
StyI CCWWGG 4 cut(s) 86, 185, 667, 815
TaaI ACNGT 2 cut(s) 30, 54
TaiI ACGT 2 cut(s) 663, 811
TaqI TCGA 2 cut(s) 231, 626
TasI AATT 7 cut(s) 166, 284, 375, 552, 867, 915, 946
TauI GCSGC 1 cut(s) 884
TfiI GAWTC 2 cut(s) 323, 934
Tru1I TTAA 7 cut(s) 129, 279, 374, 504, 903, 909, 918
Tru9I TTAA 7 cut(s) 129, 279, 374, 504, 903, 909, 918
TscAI CASTG 2 cut(s) 33, 200
TseI GCWGC 1 cut(s) 774
TspDTI ATGAA 4 cut(s) 344, 359, 736, 813
TspGWI ACGGA 1 cut(s) 90
TspRI CASTG 2 cut(s) 33, 200
VpaK11BI GGWCC 2 cut(s) 17, 260
XbaI TCTAGA 1 cut(s) 176
XceI RCATGY 3 cut(s) 349, 527, 694
XspI CTAG 3 cut(s) 177, 308, 842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.