Rw2G030900

Rhomboid-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0016892
Physical Location & Seq
Forward (+)
50776299 .. 50778315
2017 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G030900.1

Sequence Viewer

Length: 915 bp
ATGTATTATCCACCAGGTCCGCCTCCACTGTATTATCCACCACCCCCGCCACAGTTTTACGCCCCACCGCCCTTGCCACCACCCCCAAGGCGATACTCTCCGTGGCTTGTGCCTCTCATATGCTTGGTTAATCTTGGAATGTTTGTGTACGAAATGTATAAGAACAATTGCCCATCTAGACACCCAAGGGATCAGTGTATGTTAGGTGATCCTTTAGGCAGGTTCTCATTCGAACCCTTCAAAGAAGTCAAATGGATTGGTCCTTCCGCAGAAACTATTAAGAAATTAGGAGGCCTCGATCAGCAACTAGTTGTGAAAGGTGAATCATGGCGCCTGTTCTCTTGCATGTGGCTTCATGCTGGAGTTATTCATTTAATTTTCAACATACTCAGCCTTGATTTCGTTGGACTCCGACTTGAGCGCGACTTTGGATATCATAGATTTGTGTTTGTGTATGTGCTTGCTGGGTTGGGCGGAAGTGTAGCATCTTGTCTTCCTGGCGCATCTGGGGCAATTTTTGGATTGTGTGGAGCCTCCCTTTCTGAGCTGATCACAAACTGGACAATCTATGATGATAAGTGCTCATCGATCATGATACTCGTTCTCAATCTTGTCTTGAACGTGGCCCTTGGATTTCTACTGAAAATGGACATGTCAGGTCATATAGGAGGTTTTGTAGCTGGGTTCTTCCTCGGTTTTGTTTTTTTCATCAAGCCTCAGTTTGGATATATAAACAGCAAGTTTATTCCAGCATACCATGAAGTCAAACGTACACCAAGGCACAACTTCTGTCAATATTTCCTAGCGATTGTAGGTCTTGTCGGATCAATTGCTCTATTTGCGGCTGGCTTCGGTAAGCTATTTAACATTAAGGAAATTAAAGAGAAGTTGCCGAATCCCGTCAACAATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

304

Amino Acids

34.1

Weight (kDa)

9.03

Isoelectric Point (pI)

47.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 107 - 236 8.3e-32 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016892)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g29980
malus_domestica MD00G1022400.v1.1 MD00G1143800.v1.1
prunus_persica Prupe.3G083700_v2.0.a1
pyrus_communis pycom17g21420
rosa_chinensis RchiOBHm_Chr2g0135991
rosa_laevigata RLG00000019495
rosa_roxburghii Rroxscaffold_2G00108710
rosa_rugosa Rorug02G0327200
rosa_samantha Rh2AG379200 Rh2BG385700 Rh2CG364800 Rh2DG402000
rosa_wichuraiana Rw2G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 210
AccB1I GGYRCC 1 cut(s) 330
AccII CGCG 1 cut(s) 423
AciI CCGC 6 cut(s) 20, 47, 68, 267, 474, 842
AclWI GGATC 3 cut(s) 198, 203, 832
AcyI GRCGYC 1 cut(s) 331
AfaI GTAC 2 cut(s) 149, 772
AfiI CCNNNNNNNGG 1 cut(s) 722
AflIII ACRYGT 1 cut(s) 651
AgsI TTSAA 3 cut(s) 241, 382, 619
AhlI ACTAGT 1 cut(s) 307
AjnI CCWGG 2 cut(s) 13, 496
AluBI AGCT 3 cut(s) 547, 680, 859
AluI AGCT 3 cut(s) 547, 680, 859
Alw21I GWGCWC 1 cut(s) 584
AlwI GGATC 3 cut(s) 198, 203, 832
AoxI GGCC 2 cut(s) 292, 624
AspLEI GCGC 3 cut(s) 333, 423, 503
AspS9I GGNCC 3 cut(s) 17, 260, 625
AsuHPI GGTGA 2 cut(s) 218, 332
AsuII TTCGAA 1 cut(s) 231
AvaII GGWCC 2 cut(s) 17, 260
BanI GGYRCC 1 cut(s) 330
BbsI GAAGAC 1 cut(s) 485
Bbv12I GWGCWC 1 cut(s) 584
BccI CCATC 1 cut(s) 181
BciT130I CCWGG 2 cut(s) 15, 498
BclI TGATCA 1 cut(s) 549
BcuI ACTAGT 1 cut(s) 307
BfaI CTAG 3 cut(s) 177, 308, 803
BfoI RGCGCY 1 cut(s) 334
BfuAI ACCTGC 1 cut(s) 210
BisI GCNGC 1 cut(s) 843
BlsI GCNGC 1 cut(s) 844
Bme1390I CCNGG 2 cut(s) 15, 498
Bme18I GGWCC 2 cut(s) 17, 260
BmgT120I GGNCC 3 cut(s) 17, 260, 625
BmiI GGNNCC 2 cut(s) 332, 532
BmrFI CCNGG 2 cut(s) 15, 498
BmsI GCATC 2 cut(s) 494, 512
BpiI GAAGAC 1 cut(s) 485
BpmI CTGGAG 1 cut(s) 381
Bpu14I TTCGAA 1 cut(s) 231
BpuEI CTTGAG 1 cut(s) 437
Bsa29I ATCGAT 1 cut(s) 587
BsaHI GRCGYC 1 cut(s) 331
BsaJI CCNNGG 6 cut(s) 86, 101, 185, 628, 691, 776
BsaXI ACNNNNNCTCC 2 cut(s) 660, 690
Bsc4I CCNNNNNNNGG 1 cut(s) 722
Bse1I ACTGG 1 cut(s) 563
BseBI CCWGG 2 cut(s) 15, 498
BseCI ATCGAT 1 cut(s) 587
BseDI CCNNGG 6 cut(s) 86, 101, 185, 628, 691, 776
BseLI CCNNNNNNNGG 1 cut(s) 722
BseMII CTCAG 3 cut(s) 403, 534, 731
BseNI ACTGG 1 cut(s) 563
BseYI CCCAGC 2 cut(s) 464, 680
Bsh1236I CGCG 1 cut(s) 423
BshFI GGCC 2 cut(s) 294, 626
BshNI GGYRCC 1 cut(s) 330
BshVI ATCGAT 1 cut(s) 587
BsiHKAI GWGCWC 1 cut(s) 584
BslI CCNNNNNNNGG 1 cut(s) 722
BsnI GGCC 2 cut(s) 294, 626
Bsp119I TTCGAA 1 cut(s) 231
Bsp1286I GDGCHC 1 cut(s) 584
Bsp143I GATC 6 cut(s) 190, 208, 298, 549, 588, 824
BspACI CCGC 6 cut(s) 20, 47, 68, 267, 474, 842
BspANI GGCC 2 cut(s) 294, 626
BspCNI CTCAG 3 cut(s) 402, 535, 730
BspDI ATCGAT 1 cut(s) 587
BspFNI CGCG 1 cut(s) 423
BspHI TCATGA 1 cut(s) 591
BspLI GGNNCC 2 cut(s) 332, 532
BspMI ACCTGC 1 cut(s) 210
BspPI GGATC 3 cut(s) 198, 203, 832
BspT104I TTCGAA 1 cut(s) 231
BspT107I GGYRCC 1 cut(s) 330
BsrI ACTGG 1 cut(s) 563
BssECI CCNNGG 6 cut(s) 86, 101, 185, 628, 691, 776
BssMI GATC 6 cut(s) 190, 208, 298, 549, 588, 824
BssNI GRCGYC 1 cut(s) 331
BssT1I CCWWGG 4 cut(s) 86, 185, 628, 776
Bst2UI CCWGG 2 cut(s) 15, 498
Bst4CI ACNGT 2 cut(s) 30, 54
BstACI GRCGYC 1 cut(s) 331
BstBI TTCGAA 1 cut(s) 231
BstC8I GCNNGC 2 cut(s) 462, 847
BstDEI CTNAG 3 cut(s) 389, 543, 717
BstDSI CCRYGG 1 cut(s) 101
BstFNI CGCG 1 cut(s) 423
BstH2I RGCGCY 1 cut(s) 334
BstHHI GCGC 3 cut(s) 333, 423, 503
BstKTI GATC 6 cut(s) 193, 211, 301, 552, 591, 827
BstMBI GATC 6 cut(s) 190, 208, 298, 549, 588, 824
BstMWI GCNNNNNNNGC 2 cut(s) 509, 839
BstNI CCWGG 2 cut(s) 15, 498
BstNSI RCATGY 2 cut(s) 349, 655
BstSCI CCNGG 2 cut(s) 13, 496
BstUI CGCG 1 cut(s) 423
BstV2I GAAGAC 1 cut(s) 485
Bsu15I ATCGAT 1 cut(s) 587
BsuRI GGCC 2 cut(s) 294, 626
BsuTUI ATCGAT 1 cut(s) 587
BtgI CCRYGG 1 cut(s) 101
BtsIMutI CAGTG 2 cut(s) 26, 200
BveI ACCTGC 1 cut(s) 210
Cac8I GCNNGC 2 cut(s) 462, 847
CciI TCATGA 1 cut(s) 591
CfoI GCGC 3 cut(s) 333, 423, 503
Cfr13I GGNCC 3 cut(s) 17, 260, 625
ClaI ATCGAT 1 cut(s) 587
CsiI ACCWGGT 1 cut(s) 13
Csp6I GTAC 2 cut(s) 148, 771
CspCI CAANNNNNGTGG 2 cut(s) 54, 89
CviAII CATG 6 cut(s) 327, 346, 356, 592, 652, 758
CviQI GTAC 2 cut(s) 148, 771
DdeI CTNAG 3 cut(s) 389, 543, 717
DinI GGCGCC 1 cut(s) 332
DpnI GATC 6 cut(s) 192, 210, 300, 551, 590, 826
DpnII GATC 6 cut(s) 190, 208, 298, 549, 588, 824
EciI GGCGGA 2 cut(s) 9, 489
Eco130I CCWWGG 4 cut(s) 86, 185, 628, 776
Eco147I AGGCCT 1 cut(s) 294
Eco32I GATATC 1 cut(s) 434
Eco47I GGWCC 2 cut(s) 17, 260
EcoRII CCWGG 2 cut(s) 13, 496
EcoRV GATATC 1 cut(s) 434
EcoT14I CCWWGG 4 cut(s) 86, 185, 628, 776
EgeI GGCGCC 1 cut(s) 332
EheI GGCGCC 1 cut(s) 332
ErhI CCWWGG 4 cut(s) 86, 185, 628, 776
FaeI CATG 6 cut(s) 330, 349, 359, 595, 655, 761
FatI CATG 6 cut(s) 326, 345, 355, 591, 651, 757
FauI CCCGC 1 cut(s) 54
FauNDI CATATG 1 cut(s) 119
FbaI TGATCA 1 cut(s) 549
Fnu4HI GCNGC 1 cut(s) 843
Fsp4HI GCNGC 1 cut(s) 843
FspBI CTAG 3 cut(s) 177, 308, 803
GlaI GCGC 3 cut(s) 332, 422, 502
GluI GCNGC 1 cut(s) 843
GsaI CCCAGC 2 cut(s) 468, 684
GsuI CTGGAG 1 cut(s) 381
HaeII RGCGCY 1 cut(s) 334
HaeIII GGCC 2 cut(s) 294, 626
HhaI GCGC 3 cut(s) 333, 423, 503
Hin1I GRCGYC 1 cut(s) 331
Hin1II CATG 6 cut(s) 330, 349, 359, 595, 655, 761
Hin6I GCGC 3 cut(s) 331, 421, 501
HinP1I GCGC 3 cut(s) 331, 421, 501
HincII GTYRAC 1 cut(s) 904
HindII GTYRAC 1 cut(s) 904
HinfI GANTC 3 cut(s) 323, 408, 895
HphI GGTGA 2 cut(s) 218, 332
Hpy166II GTNNAC 3 cut(s) 148, 773, 904
Hpy188I TCNGA 3 cut(s) 413, 544, 824
Hpy188III TCNNGA 3 cut(s) 177, 592, 616
Hpy8I GTNNAC 3 cut(s) 148, 773, 904
HpyAV CCTTC 2 cut(s) 247, 273
HpyCH4III ACNGT 2 cut(s) 30, 54
HpyCH4IV ACGT 2 cut(s) 621, 769
HpyCH4V TGCA 1 cut(s) 345
HpyF10VI GCNNNNNNNGC 2 cut(s) 509, 839
HpyF3I CTNAG 3 cut(s) 389, 543, 717
HpySE526I ACGT 2 cut(s) 621, 769
Hsp92I GRCGYC 1 cut(s) 331
Hsp92II CATG 6 cut(s) 330, 349, 359, 595, 655, 761
HspAI GCGC 3 cut(s) 331, 421, 501
KasI GGCGCC 1 cut(s) 330
Ksp22I TGATCA 1 cut(s) 549
Kzo9I GATC 6 cut(s) 190, 208, 298, 549, 588, 824
LmnI GCTCC 1 cut(s) 530
LweI GCATC 2 cut(s) 494, 512
MabI ACCWGGT 1 cut(s) 13
MaeI CTAG 3 cut(s) 177, 308, 803
MaeII ACGT 2 cut(s) 621, 769
MalI GATC 6 cut(s) 192, 210, 300, 551, 590, 826
MboI GATC 6 cut(s) 190, 208, 298, 549, 588, 824
MboII GAAGA 2 cut(s) 485, 679
MfeI CAATTG 2 cut(s) 166, 828
MhlI GDGCHC 1 cut(s) 584
MluCI AATT 7 cut(s) 166, 284, 375, 513, 828, 876, 907
Mly113I GGCGCC 1 cut(s) 331
MlyI GAGTC 1 cut(s) 402
MmeI TCCRAC 3 cut(s) 385, 436, 802
MnlI CCTC 8 cut(s) 33, 123, 284, 305, 544, 662, 701, 726
MseI TTAA 6 cut(s) 129, 279, 374, 864, 870, 879
MspR9I CCNGG 2 cut(s) 15, 498
MunI CAATTG 2 cut(s) 166, 828
MvaI CCWGG 2 cut(s) 15, 498
MvnI CGCG 1 cut(s) 423
MwoI GCNNNNNNNGC 2 cut(s) 509, 839
NarI GGCGCC 1 cut(s) 331
NdeI CATATG 1 cut(s) 119
NdeII GATC 6 cut(s) 190, 208, 298, 549, 588, 824
NlaIII CATG 6 cut(s) 330, 349, 359, 595, 655, 761
NlaIV GGNNCC 2 cut(s) 332, 532
NspI RCATGY 2 cut(s) 349, 655
NspV TTCGAA 1 cut(s) 231
PagI TCATGA 1 cut(s) 591
PceI AGGCCT 1 cut(s) 294
PciI ACATGT 1 cut(s) 651
PfeI GAWTC 2 cut(s) 323, 895
PkrI GCNGC 1 cut(s) 844
PleI GAGTC 1 cut(s) 402
PluTI GGCGCC 1 cut(s) 334
PpsI GAGTC 1 cut(s) 402
PscI ACATGT 1 cut(s) 651
Psp6I CCWGG 2 cut(s) 13, 496
PspFI CCCAGC 2 cut(s) 464, 680
PspGI CCWGG 2 cut(s) 13, 496
PspN4I GGNNCC 2 cut(s) 332, 532
PspPI GGNCC 3 cut(s) 17, 260, 625
RsaI GTAC 2 cut(s) 149, 772
RsaNI GTAC 2 cut(s) 148, 771
SaqAI TTAA 6 cut(s) 129, 279, 374, 864, 870, 879
SatI GCNGC 1 cut(s) 843
Sau3AI GATC 6 cut(s) 190, 208, 298, 549, 588, 824
Sau96I GGNCC 3 cut(s) 17, 260, 625
SchI GAGTC 1 cut(s) 402
ScrFI CCNGG 2 cut(s) 15, 498
SduI GDGCHC 1 cut(s) 584
SexAI ACCWGGT 1 cut(s) 13
SfaNI GCATC 2 cut(s) 494, 512
SfoI GGCGCC 1 cut(s) 332
SfuI TTCGAA 1 cut(s) 231
SinI GGWCC 2 cut(s) 17, 260
SmlI CTYRAG 1 cut(s) 416
SmoI CTYRAG 1 cut(s) 416
SpeI ACTAGT 1 cut(s) 307
Sse9I AATT 7 cut(s) 166, 284, 375, 513, 828, 876, 907
SseBI AGGCCT 1 cut(s) 294
SsiI CCGC 6 cut(s) 20, 47, 68, 267, 474, 842
SspDI GGCGCC 1 cut(s) 330
SspI AATATT 1 cut(s) 797
SspMI CTAG 3 cut(s) 177, 308, 803
StuI AGGCCT 1 cut(s) 294
StyD4I CCNGG 2 cut(s) 13, 496
StyI CCWWGG 4 cut(s) 86, 185, 628, 776
TaaI ACNGT 2 cut(s) 30, 54
TaiI ACGT 2 cut(s) 624, 772
TaqI TCGA 3 cut(s) 231, 297, 587
TasI AATT 7 cut(s) 166, 284, 375, 513, 828, 876, 907
TauI GCSGC 1 cut(s) 845
TfiI GAWTC 2 cut(s) 323, 895
Tru1I TTAA 6 cut(s) 129, 279, 374, 864, 870, 879
Tru9I TTAA 6 cut(s) 129, 279, 374, 864, 870, 879
TscAI CASTG 2 cut(s) 33, 200
TspDTI ATGAA 4 cut(s) 344, 359, 697, 774
TspGWI ACGGA 1 cut(s) 90
TspRI CASTG 2 cut(s) 33, 200
VpaK11BI GGWCC 2 cut(s) 17, 260
XbaI TCTAGA 1 cut(s) 176
XceI RCATGY 2 cut(s) 349, 655
XspI CTAG 3 cut(s) 177, 308, 803
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.