MD00G1143800.v1.1

Rhomboid-like protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0016892
Physical Location & Seq
Reverse (-)
31301750 .. 31304102
2353 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1143800.v1.1.491

Sequence Viewer

Length: 942 bp
ATGTATCCGCCACCGCCATACTATCCTCCACCTCCACCAGCCTTTTACGCGCCGCCACCAATGATGGCACCACCAAGGCGGTACTCTCCGTGGCTGGTGCCGCTTATATTCATTGTGAATATCGGGGTGTTCATATGGACAATGTATGAGAACAACTGCCCATCTAAAATGTCAAAGGATCAGTGTATGGGAGGGCATTACTTGGATAGGTTCTCCTTCCAACCCTGGCATGATAACCGCATGATCGGTCCTACCCCTGAAACCCTCCAAAGACTAGGAGGCCTTGATACAAAACTAGTGCTGAATGGTGAAGCATGGCGCCTGTTTTCTGCAACGTGGCTACATGGCGGGCTGATTCATCTATTCGCCAACATGATCAGCCTTGATTTCGTAGGGCTCCGGCTTGAGCAGGACTTTGGATTTCACAGATTCGGATTTGTGTATGTGCTTTCTGGCTTAGGGGGAAGTTTAGGGTCTTGCCTTAATATTATTAAACACGGAAAGTCAAGCAAGACTATATCAGTTGGCGCATCTGGGGCAATTTTTGGATTGTTGGGAGCCTCACTTTCTGAGCTGATCACAAACTGGACAGTGTATGACGATAAGTGCTCGTCGATCATGATAATCATCCTAAATGCTGCCCTGAACTTGGCCATTGGATTTCTACTTCATATGGACCATTCAGCTCATGTAGGAGGATTCGTCGCCGGATTTTTCCTTGGTTTTGTGTTTTTTGTTAAGCCTCAGTTTGGGTATGTAAGCAGCAAGTACATGCCAACCTACCATCAAGTCAAAAGTACAGCAAGGCACAATTTCTGTCAATATTTTCTGGGGTTCGTTGGTTTGGTGGCGTGCATTATTCTGTATGCAACTGCCTTCGGTAAGCTATTTAATATAAGGGAAATTAAACAACATCTGCCTGATAGTATAAACAAATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhomboid

Protein Analysis

314

Amino Acids

35.05

Weight (kDa)

9.05

Isoelectric Point (pI)

48.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhomboid PF01694 103 - 245 9.8e-36 Rhomboid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016892)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g29980
malus_domestica MD00G1022400.v1.1 MD00G1143800.v1.1
prunus_persica Prupe.3G083700_v2.0.a1
pyrus_communis pycom17g21420
rosa_chinensis RchiOBHm_Chr2g0135991
rosa_laevigata RLG00000019495
rosa_roxburghii Rroxscaffold_2G00108710
rosa_rugosa Rorug02G0327200
rosa_samantha Rh2AG379200 Rh2BG385700 Rh2CG364800 Rh2DG402000
rosa_wichuraiana Rw2G030900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 67, 97, 318
AccII CGCG 1 cut(s) 50
AciI CCGC 7 cut(s) 8, 14, 53, 79, 101, 238, 348
AclWI GGATC 1 cut(s) 186
AcoI YGGCCR 1 cut(s) 651
AcyI GRCGYC 1 cut(s) 319
AfaI GTAC 3 cut(s) 83, 770, 799
AfiI CCNNNNNNNGG 2 cut(s) 649, 749
AhlI ACTAGT 1 cut(s) 295
AjnI CCWGG 1 cut(s) 224
AluBI AGCT 3 cut(s) 574, 686, 886
AluI AGCT 3 cut(s) 574, 686, 886
Alw21I GWGCWC 1 cut(s) 611
AlwI GGATC 1 cut(s) 186
AoxI GGCC 2 cut(s) 280, 651
ApeKI GCWGC 2 cut(s) 638, 762
AspLEI GCGC 3 cut(s) 52, 321, 530
AspS9I GGNCC 2 cut(s) 248, 676
AsuHPI GGTGA 1 cut(s) 320
AvaII GGWCC 2 cut(s) 248, 676
BalI TGGCCA 1 cut(s) 653
BanI GGYRCC 3 cut(s) 67, 97, 318
BanII GRGCYC 1 cut(s) 399
Bbv12I GWGCWC 1 cut(s) 611
BbvI GCAGC 2 cut(s) 625, 774
BccI CCATC 3 cut(s) 58, 169, 792
BciT130I CCWGG 1 cut(s) 226
BciVI GTATCC 1 cut(s) 15
BclI TGATCA 2 cut(s) 375, 576
BcuI ACTAGT 1 cut(s) 295
BfaI CTAG 2 cut(s) 275, 296
BfoI RGCGCY 1 cut(s) 322
BfuI GTATCC 1 cut(s) 15
BisI GCNGC 4 cut(s) 53, 101, 639, 763
BlsI GCNGC 4 cut(s) 54, 102, 640, 764
Bme1390I CCNGG 1 cut(s) 226
Bme18I GGWCC 2 cut(s) 248, 676
BmgT120I GGNCC 2 cut(s) 248, 676
BmiI GGNNCC 5 cut(s) 69, 99, 320, 398, 559
BmrFI CCNGG 1 cut(s) 226
BmsI GCATC 1 cut(s) 539
Bpu10I CCTNAGC 1 cut(s) 457
BpuEI CTTGAG 1 cut(s) 425
BsaBI GATNNNNATC 1 cut(s) 626
BsaHI GRCGYC 1 cut(s) 319
BsaJI CCNNGG 4 cut(s) 74, 89, 224, 718
BsaXI ACNNNNNCTCC 2 cut(s) 687, 717
Bsc4I CCNNNNNNNGG 2 cut(s) 649, 749
Bse1I ACTGG 1 cut(s) 590
Bse8I GATNNNNATC 1 cut(s) 626
BseBI CCWGG 1 cut(s) 226
BseDI CCNNGG 4 cut(s) 74, 89, 224, 718
BseGI GGATG 1 cut(s) 627
BseJI GATNNNNATC 1 cut(s) 626
BseLI CCNNNNNNNGG 2 cut(s) 649, 749
BseMII CTCAG 2 cut(s) 561, 758
BseNI ACTGG 1 cut(s) 590
BseXI GCAGC 2 cut(s) 625, 774
Bsh1236I CGCG 1 cut(s) 50
BshFI GGCC 2 cut(s) 282, 653
BshNI GGYRCC 3 cut(s) 67, 97, 318
BsiHKAI GWGCWC 1 cut(s) 611
BsiSI CCGG 2 cut(s) 400, 708
BslI CCNNNNNNNGG 2 cut(s) 649, 749
BsnI GGCC 2 cut(s) 282, 653
Bsp1286I GDGCHC 2 cut(s) 399, 611
Bsp143I GATC 5 cut(s) 178, 243, 375, 576, 615
BspACI CCGC 7 cut(s) 8, 14, 53, 79, 101, 238, 348
BspANI GGCC 2 cut(s) 282, 653
BspCNI CTCAG 2 cut(s) 562, 757
BspFNI CGCG 1 cut(s) 50
BspHI TCATGA 1 cut(s) 618
BspLI GGNNCC 5 cut(s) 69, 99, 320, 398, 559
BspPI GGATC 1 cut(s) 186
BspT107I GGYRCC 3 cut(s) 67, 97, 318
BsrI ACTGG 1 cut(s) 590
BssECI CCNNGG 4 cut(s) 74, 89, 224, 718
BssMI GATC 5 cut(s) 178, 243, 375, 576, 615
BssNI GRCGYC 1 cut(s) 319
BssT1I CCWWGG 2 cut(s) 74, 718
Bst2UI CCWGG 1 cut(s) 226
Bst4CI ACNGT 1 cut(s) 592
BstACI GRCGYC 1 cut(s) 319
BstC8I GCNNGC 2 cut(s) 350, 853
BstDEI CTNAG 3 cut(s) 457, 570, 744
BstDSI CCRYGG 1 cut(s) 89
BstF5I GGATG 1 cut(s) 627
BstFNI CGCG 1 cut(s) 50
BstH2I RGCGCY 1 cut(s) 322
BstHHI GCGC 3 cut(s) 52, 321, 530
BstKTI GATC 5 cut(s) 181, 246, 378, 579, 618
BstMBI GATC 5 cut(s) 178, 243, 375, 576, 615
BstMWI GCNNNNNNNGC 3 cut(s) 47, 100, 536
BstNI CCWGG 1 cut(s) 226
BstNSI RCATGY 1 cut(s) 775
BstSCI CCNGG 1 cut(s) 224
BstUI CGCG 1 cut(s) 50
BstV1I GCAGC 2 cut(s) 625, 774
BsuI GTATCC 1 cut(s) 15
BsuRI GGCC 2 cut(s) 282, 653
BtgI CCRYGG 1 cut(s) 89
BtsCI GGATG 1 cut(s) 627
BtsIMutI CAGTG 2 cut(s) 188, 597
Cac8I GCNNGC 2 cut(s) 350, 853
CciI TCATGA 1 cut(s) 618
CfoI GCGC 3 cut(s) 52, 321, 530
Cfr13I GGNCC 2 cut(s) 248, 676
Csp6I GTAC 3 cut(s) 82, 769, 798
CviAII CATG 8 cut(s) 230, 241, 315, 344, 373, 619, 689, 772
CviQI GTAC 3 cut(s) 82, 769, 798
DdeI CTNAG 3 cut(s) 457, 570, 744
DinI GGCGCC 1 cut(s) 320
DpnI GATC 5 cut(s) 180, 245, 377, 578, 617
DpnII GATC 5 cut(s) 178, 243, 375, 576, 615
EaeI YGGCCR 1 cut(s) 651
Eco130I CCWWGG 2 cut(s) 74, 718
Eco147I AGGCCT 1 cut(s) 282
Eco24I GRGCYC 1 cut(s) 399
Eco47I GGWCC 2 cut(s) 248, 676
EcoRII CCWGG 1 cut(s) 224
EcoT14I CCWWGG 2 cut(s) 74, 718
EcoT38I GRGCYC 1 cut(s) 399
EgeI GGCGCC 1 cut(s) 320
EheI GGCGCC 1 cut(s) 320
ErhI CCWWGG 2 cut(s) 74, 718
FaeI CATG 8 cut(s) 233, 244, 318, 347, 376, 622, 692, 775
FatI CATG 8 cut(s) 229, 240, 314, 343, 372, 618, 688, 771
FauI CCCGC 1 cut(s) 341
FauNDI CATATG 2 cut(s) 134, 672
FbaI TGATCA 2 cut(s) 375, 576
Fnu4HI GCNGC 4 cut(s) 53, 101, 639, 763
FokI GGATG 1 cut(s) 614
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 4 cut(s) 53, 101, 639, 763
FspBI CTAG 2 cut(s) 275, 296
GlaI GCGC 3 cut(s) 51, 320, 529
GluI GCNGC 4 cut(s) 53, 101, 639, 763
HaeII RGCGCY 1 cut(s) 322
HaeIII GGCC 2 cut(s) 282, 653
HapII CCGG 2 cut(s) 400, 708
HhaI GCGC 3 cut(s) 52, 321, 530
Hin1I GRCGYC 1 cut(s) 319
Hin1II CATG 8 cut(s) 233, 244, 318, 347, 376, 622, 692, 775
Hin6I GCGC 3 cut(s) 50, 319, 528
HinP1I GCGC 3 cut(s) 50, 319, 528
HinfI GANTC 3 cut(s) 355, 429, 699
HpaII CCGG 2 cut(s) 400, 708
HphI GGTGA 1 cut(s) 320
Hpy188I TCNGA 2 cut(s) 434, 571
Hpy188III TCNNGA 1 cut(s) 619
Hpy99I CGWCG 2 cut(s) 616, 707
HpyAV CCTTC 2 cut(s) 226, 886
HpyCH4III ACNGT 1 cut(s) 592
HpyCH4IV ACGT 1 cut(s) 335
HpyCH4V TGCA 3 cut(s) 332, 855, 869
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 100, 536
HpyF3I CTNAG 3 cut(s) 457, 570, 744
HpySE526I ACGT 1 cut(s) 335
Hsp92I GRCGYC 1 cut(s) 319
Hsp92II CATG 8 cut(s) 233, 244, 318, 347, 376, 622, 692, 775
HspAI GCGC 3 cut(s) 50, 319, 528
KasI GGCGCC 1 cut(s) 318
Ksp22I TGATCA 2 cut(s) 375, 576
Kzo9I GATC 5 cut(s) 178, 243, 375, 576, 615
LmnI GCTCC 2 cut(s) 402, 557
Lsp1109I GCAGC 2 cut(s) 625, 774
LweI GCATC 1 cut(s) 539
MaeI CTAG 2 cut(s) 275, 296
MaeII ACGT 1 cut(s) 335
MalI GATC 5 cut(s) 180, 245, 377, 578, 617
MboI GATC 5 cut(s) 178, 243, 375, 576, 615
MhlI GDGCHC 2 cut(s) 399, 611
MlsI TGGCCA 1 cut(s) 653
MluCI AATT 3 cut(s) 540, 811, 903
MluNI TGGCCA 1 cut(s) 653
Mly113I GGCGCC 1 cut(s) 319
MmeI TCCRAC 1 cut(s) 244
MnlI CCTC 8 cut(s) 36, 42, 185, 272, 275, 571, 689, 753
Mox20I TGGCCA 1 cut(s) 653
MscI TGGCCA 1 cut(s) 653
MseI TTAA 5 cut(s) 483, 492, 738, 891, 906
Msp20I TGGCCA 1 cut(s) 653
MspI CCGG 2 cut(s) 400, 708
MspR9I CCNGG 1 cut(s) 226
MvaI CCWGG 1 cut(s) 226
MvnI CGCG 1 cut(s) 50
MwoI GCNNNNNNNGC 3 cut(s) 47, 100, 536
NarI GGCGCC 1 cut(s) 319
NdeI CATATG 2 cut(s) 134, 672
NdeII GATC 5 cut(s) 178, 243, 375, 576, 615
NlaIII CATG 8 cut(s) 233, 244, 318, 347, 376, 622, 692, 775
NlaIV GGNNCC 5 cut(s) 69, 99, 320, 398, 559
NspI RCATGY 1 cut(s) 775
PagI TCATGA 1 cut(s) 618
PceI AGGCCT 1 cut(s) 282
PfeI GAWTC 3 cut(s) 355, 429, 699
PkrI GCNGC 4 cut(s) 54, 102, 640, 764
PluTI GGCGCC 1 cut(s) 322
Psp6I CCWGG 1 cut(s) 224
PspGI CCWGG 1 cut(s) 224
PspN4I GGNNCC 5 cut(s) 69, 99, 320, 398, 559
PspPI GGNCC 2 cut(s) 248, 676
RsaI GTAC 3 cut(s) 83, 770, 799
RsaNI GTAC 3 cut(s) 82, 769, 798
SaqAI TTAA 5 cut(s) 483, 492, 738, 891, 906
SatI GCNGC 4 cut(s) 53, 101, 639, 763
Sau3AI GATC 5 cut(s) 178, 243, 375, 576, 615
Sau96I GGNCC 2 cut(s) 248, 676
ScrFI CCNGG 1 cut(s) 226
SduI GDGCHC 2 cut(s) 399, 611
SetI ASST 7 cut(s) 34, 212, 338, 576, 688, 782, 888
SfaNI GCATC 1 cut(s) 539
SfoI GGCGCC 1 cut(s) 320
SinI GGWCC 2 cut(s) 248, 676
SmlI CTYRAG 1 cut(s) 404
SmoI CTYRAG 1 cut(s) 404
SpeI ACTAGT 1 cut(s) 295
Sse9I AATT 3 cut(s) 540, 811, 903
SseBI AGGCCT 1 cut(s) 282
SsiI CCGC 7 cut(s) 8, 14, 53, 79, 101, 238, 348
SspDI GGCGCC 1 cut(s) 318
SspI AATATT 3 cut(s) 487, 824, 938
SspMI CTAG 2 cut(s) 275, 296
StuI AGGCCT 1 cut(s) 282
StyD4I CCNGG 1 cut(s) 224
StyI CCWWGG 2 cut(s) 74, 718
TaaI ACNGT 1 cut(s) 592
TaiI ACGT 1 cut(s) 338
TaqI TCGA 1 cut(s) 614
TaqII GACCGA 1 cut(s) 236
TasI AATT 3 cut(s) 540, 811, 903
TatI WGTACW 2 cut(s) 768, 797
TauI GCSGC 2 cut(s) 55, 103
TfiI GAWTC 3 cut(s) 355, 429, 699
Tru1I TTAA 5 cut(s) 483, 492, 738, 891, 906
Tru9I TTAA 5 cut(s) 483, 492, 738, 891, 906
TscAI CASTG 2 cut(s) 188, 597
TseI GCWGC 2 cut(s) 638, 762
TspDTI ATGAA 4 cut(s) 100, 121, 347, 659
TspGWI ACGGA 2 cut(s) 78, 513
TspRI CASTG 2 cut(s) 188, 597
VpaK11BI GGWCC 2 cut(s) 248, 676
XceI RCATGY 1 cut(s) 775
XspI CTAG 2 cut(s) 275, 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.