Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0015121

Physical Location & Seq

Genomic Coordinates Reverse (-)

23952467 .. 23958122

Gene Structure 5656 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g30820.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1599 bp

ATGGTGGGTCCGACGCTGGTGGGTCTGCAAGACCACCTGAAATTGGCGAGAGAGTACGCCATGGAAGGCCTCTACGACACCTCCATGATCTTCTTCGATGGCGGCATTGCTCAGATCAACAAGCACATAAACACGCTTGATGATCCGTTGATTCGCTCAAAATGGATGAATGTAAAGAAGGCACTGTCCGAGGAAGCAGAGGTTGTGAAACAACTAGATGCAGAGAGAAAGGCATTTAAGGAAAGCCCCATGGGCCGACGCCCTTCCTCACCTCCAATTCATGCCAAGTCATCCTTCGTTTTTCAACCCTTAGATGAATACCCCACTTCTTCAGGATACCCAACTTCTTCAGGATACCCAACTTCTTCAGGTTCTCCTATGGATGATCCTGATGTGTGGAGGCCACCAAGTCGGGACACTTCAAACAGAAGAAATGCAAAGGCTGGTCAAGTTGGTATGAGGAAGTCACCGCAAGATGGAAATTGGGCTCGGAGTTCTGCATCAAAAGCAGGCACACCAGGCCGTAATGCAAAGGTTGGTGGATCAAGTAGGACCAACTCCACGGTCAGGTCTTCTACTCCCGGCAAGAAAGACACCCGATCTGGAAAATCTAGCAAGGCAGATTCAGCGAATGGTGATGCTGAGGATGGAAAGCCGAAGAGGCAGTATGAGGGACCTGATCCTGATTTGGCTGAAATGCTTGAAAGAGATGTACTGGAAACCAGTCCTGGAGTAAGATGGGATGATGTTGCTGGTCTGACTGAAGCAAAAAGACTCCTGGAGGAAGCCGTTGTTCTTCCTCTGTGGATGCCAGAATATTTTCAGGGGATCAGGAGACCATGGAAGGGTGTTCTTATGTTTGGTCCTCCTGGAACTGGAAAGACATTACTTGCTAAAGCTGTTGCTACTGAGTGTGGCACAACTTTTTTCAATGTCTCCTCTGCTACATTAGCTTCAAAGTGGCGTGGAGAAAGTGAACGTATGGTTCGGTGCCTATTTGATCTCGCAAGAGCTTACGCTCCGAGTACAATCTTTATAGATGAAATTGATTCTCTCTGCAATTCCCGGGGGGCATCAGGAGAGCATGAGTCATCCAGAAGGGTGAAATCTGAACTTCTGGTCCAGGTTGATGGTGTAAATGCTACTTCCACAAATGAAGATGGTACTCGCAAGATAGTGATGGTTCTGGCAGCTACTAACTTCCCATGGGACATTGATGAGGCTCTCAGGAGAAGGCTGGAGAAGCGTATTTATATTCCTCTTCCAAATTTTGAGAGCCGTAAAGAGCTTATTCGGATAAATTTGAAAACAGTTGAGGTTGCTCCGGATGTAAACATTGATGATGTGGCTCGCCGAACAGATGGATATAGTGGTGATGATCTCACAAACGTTTGCCGTGATGCTTCCTTGAATGGGATGAGGAGGAAAATAGCTGGAAAGACACGTGATGAGATCAAGAGCATGGCTAAGGATGAAATTTCAAAGGATCCTGTTGAAATGTGTGATTTTGAAGAAGCCTTGTCGAAGGTCCAGCGGAGTGTTTCTCAATCTGATATTGAAAAACATGAAAAATGGTTCTCAGAGTTTGGGTCAGCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0000902 GO:0000904 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006996 GO:0007010 GO:0007017 GO:0008150 GO:0009653 GO:0009825 GO:0009832 GO:0009888 GO:0009987 GO:0010026 GO:0010090 GO:0010091 GO:0016043 GO:0016049 GO:0030154 GO:0030865 GO:0031122 GO:0032502 GO:0032989 GO:0040007 GO:0042546 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043622 GO:0044085 GO:0044424 GO:0044464 GO:0048468 GO:0048856 GO:0048869 GO:0071554 GO:0071669 GO:0071840 GO:0090558 GO:0090626 GO:0097435

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

AAA Aminotran_1_2 Vps4_C

Protein Analysis

533

Amino Acids

58.84

Weight (kDa)

7.59

Isoelectric Point (pI)

52.01

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AAA	PF00004	229 - 366	2.6e-38	ATPase family associated with various cellular activities (AAA)
AAA_lid_3	PF17862	388 - 430	2.2e-12	AAA+ lid domain
Vps4_C	PF09336	430 - 475	8.2e-11	Vps4 C terminal oligomerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015121)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G80350
fragaria_vesca	FvH4_6g30820 FvH4_6g30820
malus_domestica	MD09G1216700.v1.1 MD17G1198800.v1.1
prunus_persica	Prupe.3G075400_v2.0.a1
pyrus_communis	pycom09g13400 pycom17g20350
rosa_chinensis	RchiOBHm_Chr2g0137251
rosa_laevigata	RLG00000019638
rosa_multiflora	Rmu_sc0000894.1_g000020
rosa_roxburghii	Rroxscaffold_2G00107270
rosa_rugosa	Rorug02G0339900 Rorug02G0340000
rosa_samantha	Rh2BG396400
rosa_wichuraiana	Rw2G031850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	990
AccIII	TCCGGA	1 cut(s)	1324
AciI	CCGC	3 cut(s)	102, 470, 1534
AclI	AACGTT	1 cut(s)	1389
AclWI	GGATC	7 cut(s)	137, 380, 550, 674, 836, 1481, 1494
AcsI	RAATTY	3 cut(s)	1267, 1300, 1476
AcuI	CTGAAG	4 cut(s)	315, 333, 351, 783
AcvI	CACGTG	1 cut(s)	1445
AcyI	GRCGYC	1 cut(s)	259
AfaI	GTAC	4 cut(s)	56, 714, 1027, 1165
AfiI	CCNNNNNNNGG	6 cut(s)	43, 476, 567, 845, 875, 1413
AflIII	ACRYGT	1 cut(s)	1442
AjnI	CCWGG	5 cut(s)	517, 727, 777, 868, 1122
AjuI	GAANNNNNNNTTGG	2 cut(s)	278, 310
AloI	GAACNNNNNNTCC	2 cut(s)	1104, 1136
AluBI	AGCT	6 cut(s)	899, 953, 1013, 1193, 1288, 1433
AluI	AGCT	6 cut(s)	899, 953, 1013, 1193, 1288, 1433
Alw26I	GTCTC	2 cut(s)	829, 940
AlwI	GGATC	7 cut(s)	137, 380, 550, 674, 836, 1481, 1494
Ama87I	CYCGRG	1 cut(s)	1065
Aor13HI	TCCGGA	1 cut(s)	1324
AoxI	GGCC	4 cut(s)	67, 253, 401, 520
ApeKI	GCWGC	1 cut(s)	1190
ApoI	RAATTY	3 cut(s)	1267, 1300, 1476
Asp700I	GAANNNNTTC	1 cut(s)	819
AspS9I	GGNCC	7 cut(s)	8, 253, 552, 674, 863, 1120, 1528
AsuC2I	CCSGG	3 cut(s)	582, 1066, 1067
AsuHPI	GGTGA	5 cut(s)	261, 459, 647, 1114, 1385
AvaI	CYCGRG	1 cut(s)	1065
AvaII	GGWCC	6 cut(s)	8, 552, 674, 863, 1120, 1528
BamHI	GGATCC	1 cut(s)	1486
BanI	GGYRCC	1 cut(s)	990
BanII	GRGCYC	1 cut(s)	490
BbrPI	CACGTG	1 cut(s)	1445
BbsI	GAAGAC	1 cut(s)	564
BbvCI	CCTCAGC	1 cut(s)	642
BbvI	GCAGC	1 cut(s)	1202
BccI	CCATC	8 cut(s)	92, 470, 641, 732, 1124, 1154, 1174, 1355
BceAI	ACGGC	4 cut(s)	507, 773, 1263, 1380
BciT130I	CCWGG	5 cut(s)	519, 729, 779, 870, 1124
BciVI	GTATCC	2 cut(s)	329, 347
BcnI	CCSGG	3 cut(s)	582, 1066, 1067
BcoDI	GTCTC	2 cut(s)	829, 940
BfaI	CTAG	2 cut(s)	215, 612
BfuI	GTATCC	2 cut(s)	329, 347
BglI	GCCNNNNNGGC	2 cut(s)	252, 661
BisI	GCNGC	2 cut(s)	103, 1191
BlsI	GCNGC	2 cut(s)	104, 1192
Bme1390I	CCNGG	8 cut(s)	519, 582, 729, 779, 870, 1066, 1067, 1124
Bme18I	GGWCC	6 cut(s)	8, 552, 674, 863, 1120, 1528
BmeT110I	CYCGRG	1 cut(s)	1065
BmgT120I	GGNCC	7 cut(s)	8, 253, 552, 674, 863, 1120, 1528
BmiI	GGNNCC	4 cut(s)	9, 675, 992, 1488
BmrFI	CCNGG	8 cut(s)	519, 582, 729, 779, 870, 1066, 1067, 1124
BmsI	GCATC	6 cut(s)	208, 509, 628, 798, 1082, 1390
BpiI	GAAGAC	1 cut(s)	564
BplI	GAGNNNNNCTC	2 cut(s)	1528, 1560
BpmI	CTGGAG	3 cut(s)	750, 800, 1259
Bpu10I	CCTNAGC	2 cut(s)	642, 1467
BpuMI	CCSGG	3 cut(s)	582, 1066, 1067
BsaAI	YACGTR	1 cut(s)	1445
BsaHI	GRCGYC	1 cut(s)	259
BsaI	GGTCTC	1 cut(s)	829
BsaJI	CCNNGG	8 cut(s)	60, 189, 249, 561, 839, 1065, 1066, 1205
BsaWI	WCCGGW	1 cut(s)	1324
BsaXI	ACNNNNNCTCC	2 cut(s)	65, 95
Bsc4I	CCNNNNNNNGG	6 cut(s)	43, 476, 567, 845, 875, 1413
Bse1I	ACTGG	3 cut(s)	720, 723, 880
Bse3DI	GCAATG	1 cut(s)	105
BseAI	TCCGGA	1 cut(s)	1324
BseBI	CCWGG	5 cut(s)	519, 729, 779, 870, 1124
BseDI	CCNNGG	8 cut(s)	60, 189, 249, 561, 839, 1065, 1066, 1205
BseLI	CCNNNNNNNGG	6 cut(s)	43, 476, 567, 845, 875, 1413
BseMI	GCAATG	1 cut(s)	105
BseMII	CTCAG	5 cut(s)	125, 633, 900, 1240, 1593
BseNI	ACTGG	3 cut(s)	720, 723, 880
BseRI	GAGGAG	2 cut(s)	928, 1435
BseXI	GCAGC	1 cut(s)	1202
BshFI	GGCC	4 cut(s)	69, 255, 403, 522
BshNI	GGYRCC	1 cut(s)	990
BsiHKCI	CYCGRG	1 cut(s)	1065
BsiSI	CCGG	3 cut(s)	582, 1066, 1325
BslFI	GGGAC	3 cut(s)	428, 687, 1223
BslI	CCNNNNNNNGG	6 cut(s)	43, 476, 567, 845, 875, 1413
BsmAI	GTCTC	2 cut(s)	829, 940
BsmFI	GGGAC	3 cut(s)	428, 687, 1223
BsnI	GGCC	4 cut(s)	69, 255, 403, 522
Bso31I	GGTCTC	1 cut(s)	829
BsoBI	CYCGRG	1 cut(s)	1065
Bsp1286I	GDGCHC	1 cut(s)	490
Bsp13I	TCCGGA	1 cut(s)	1324
Bsp19I	CCATGG	4 cut(s)	60, 249, 839, 1205
BspACI	CCGC	3 cut(s)	102, 470, 1534
BspANI	GGCC	4 cut(s)	69, 255, 403, 522
BspCNI	CTCAG	5 cut(s)	124, 634, 901, 1239, 1592
BspEI	TCCGGA	1 cut(s)	1324
BspLI	GGNNCC	4 cut(s)	9, 675, 992, 1488
BspPI	GGATC	7 cut(s)	137, 380, 550, 674, 836, 1481, 1494
BspT107I	GGYRCC	1 cut(s)	990
BspTNI	GGTCTC	1 cut(s)	829
BsrDI	GCAATG	1 cut(s)	105
BsrI	ACTGG	3 cut(s)	720, 723, 880
BssECI	CCNNGG	8 cut(s)	60, 189, 249, 561, 839, 1065, 1066, 1205
BssNI	GRCGYC	1 cut(s)	259
BssT1I	CCWWGG	4 cut(s)	60, 249, 839, 1205
Bst2UI	CCWGG	5 cut(s)	519, 729, 779, 870, 1124
Bst4CI	ACNGT	3 cut(s)	186, 565, 1312
Bst6I	CTCTTC	2 cut(s)	653, 1266
BstACI	GRCGYC	1 cut(s)	259
BstBAI	YACGTR	1 cut(s)	1445
BstC8I	GCNNGC	2 cut(s)	511, 1351
BstDEI	CTNAG	7 cut(s)	111, 310, 642, 909, 1226, 1467, 1579
BstDSI	CCRYGG	5 cut(s)	60, 249, 561, 839, 1205
BstMAI	GTCTC	2 cut(s)	829, 940
BstMWI	GCNNNNNNNGC	7 cut(s)	252, 506, 519, 626, 661, 950, 1243
BstNI	CCWGG	5 cut(s)	519, 729, 779, 870, 1124
BstSCI	CCNGG	8 cut(s)	517, 580, 727, 777, 868, 1064, 1065, 1122
BstV1I	GCAGC	1 cut(s)	1202
BstV2I	GAAGAC	1 cut(s)	564
BstX2I	RGATCY	1 cut(s)	1486
BstXI	CCANNNNNNTGG	1 cut(s)	1130
BstYI	RGATCY	1 cut(s)	1486
BsuI	GTATCC	2 cut(s)	329, 347
BsuRI	GGCC	4 cut(s)	69, 255, 403, 522
BtgI	CCRYGG	5 cut(s)	60, 249, 561, 839, 1205
BtsIMutI	CAGTG	1 cut(s)	182
Cac8I	GCNNGC	2 cut(s)	511, 1351
Cfr13I	GGNCC	7 cut(s)	8, 253, 552, 674, 863, 1120, 1528
Cfr9I	CCCGGG	1 cut(s)	1065
CseI	GACGC	2 cut(s)	22, 267
Csp6I	GTAC	4 cut(s)	55, 713, 1026, 1164
CviAII	CATG	9 cut(s)	61, 85, 250, 281, 840, 1085, 1206, 1462, 1565
CviQI	GTAC	4 cut(s)	55, 713, 1026, 1164
DdeI	CTNAG	7 cut(s)	111, 310, 642, 909, 1226, 1467, 1579
Eam1104I	CTCTTC	2 cut(s)	653, 1266
EarI	CTCTTC	2 cut(s)	653, 1266
Eco130I	CCWWGG	4 cut(s)	60, 249, 839, 1205
Eco147I	AGGCCT	1 cut(s)	69
Eco24I	GRGCYC	1 cut(s)	490
Eco31I	GGTCTC	1 cut(s)	829
Eco47I	GGWCC	6 cut(s)	8, 552, 674, 863, 1120, 1528
Eco57I	CTGAAG	4 cut(s)	315, 333, 351, 783
Eco72I	CACGTG	1 cut(s)	1445
Eco88I	CYCGRG	1 cut(s)	1065
EcoO109I	RGGNCCY	1 cut(s)	674
EcoRII	CCWGG	5 cut(s)	517, 727, 777, 868, 1122
EcoT14I	CCWWGG	4 cut(s)	60, 249, 839, 1205
EcoT38I	GRGCYC	1 cut(s)	490
ErhI	CCWWGG	4 cut(s)	60, 249, 839, 1205
FaeI	CATG	9 cut(s)	64, 88, 253, 284, 843, 1088, 1209, 1465, 1568
FalI	AAGNNNNNCTT	2 cut(s)	278, 310
FaqI	GGGAC	3 cut(s)	428, 687, 1223
FatI	CATG	9 cut(s)	60, 84, 249, 280, 839, 1084, 1205, 1461, 1564
Fnu4HI	GCNGC	2 cut(s)	103, 1191
FriOI	GRGCYC	1 cut(s)	490
Fsp4HI	GCNGC	2 cut(s)	103, 1191
FspBI	CTAG	2 cut(s)	215, 612
GluI	GCNGC	2 cut(s)	103, 1191
GsuI	CTGGAG	3 cut(s)	750, 800, 1259
HaeIII	GGCC	4 cut(s)	69, 255, 403, 522
HapII	CCGG	3 cut(s)	582, 1066, 1325
HgaI	GACGC	2 cut(s)	22, 267
Hin1I	GRCGYC	1 cut(s)	259
Hin1II	CATG	9 cut(s)	64, 88, 253, 284, 843, 1088, 1209, 1465, 1568
HinfI	GANTC	5 cut(s)	151, 623, 774, 1049, 1088
HpaII	CCGG	3 cut(s)	582, 1066, 1325
HphI	GGTGA	5 cut(s)	261, 459, 647, 1114, 1385
Hpy166II	GTNNAC	2 cut(s)	977, 1333
Hpy8I	GTNNAC	2 cut(s)	977, 1333
Hpy99I	CGWCG	2 cut(s)	16, 261
HpyAV	CCTTC	8 cut(s)	59, 172, 273, 304, 838, 1092, 1227, 1519
HpyCH4III	ACNGT	3 cut(s)	186, 565, 1312
HpyCH4IV	ACGT	3 cut(s)	979, 1389, 1444
HpyCH4V	TGCA	6 cut(s)	28, 221, 437, 500, 530, 1059
HpyF10VI	GCNNNNNNNGC	7 cut(s)	252, 506, 519, 626, 661, 950, 1243
HpyF3I	CTNAG	7 cut(s)	111, 310, 642, 909, 1226, 1467, 1579
HpySE526I	ACGT	3 cut(s)	979, 1389, 1444
Hsp92I	GRCGYC	1 cut(s)	259
Hsp92II	CATG	9 cut(s)	64, 88, 253, 284, 843, 1088, 1209, 1465, 1568
Kpn2I	TCCGGA	1 cut(s)	1324
LmnI	GCTCC	2 cut(s)	1024, 1327
Lsp1109I	GCAGC	1 cut(s)	1202
LweI	GCATC	6 cut(s)	208, 509, 628, 798, 1082, 1390
MaeI	CTAG	2 cut(s)	215, 612
MaeII	ACGT	3 cut(s)	979, 1389, 1444
MaeIII	GTNAC	1 cut(s)	465
MflI	RGATCY	1 cut(s)	1486
MhlI	GDGCHC	1 cut(s)	490
MluCI	AATT	8 cut(s)	41, 276, 481, 1044, 1060, 1267, 1300, 1476
MlyI	GAGTC	2 cut(s)	768, 1097
MmeI	TCCRAC	1 cut(s)	35
MroI	TCCGGA	1 cut(s)	1324
MroXI	GAANNNNTTC	1 cut(s)	819
MseI	TTAA	1 cut(s)	237
MslI	CAYNNNNRTG	1 cut(s)	83
MspA1I	CMGCKG	1 cut(s)	1534
MspI	CCGG	3 cut(s)	582, 1066, 1325
MspR9I	CCNGG	8 cut(s)	519, 582, 729, 779, 870, 1066, 1067, 1124
MvaI	CCWGG	5 cut(s)	519, 729, 779, 870, 1124
MwoI	GCNNNNNNNGC	7 cut(s)	252, 506, 519, 626, 661, 950, 1243
NciI	CCSGG	3 cut(s)	582, 1066, 1067
NcoI	CCATGG	4 cut(s)	60, 249, 839, 1205
NlaIII	CATG	9 cut(s)	64, 88, 253, 284, 843, 1088, 1209, 1465, 1568
NlaIV	GGNNCC	4 cut(s)	9, 675, 992, 1488
NmuCI	GTSAC	1 cut(s)	465
PceI	AGGCCT	1 cut(s)	69
PdmI	GAANNNNTTC	1 cut(s)	819
PfeI	GAWTC	3 cut(s)	151, 623, 1049
PfoI	TCCNGGA	3 cut(s)	727, 777, 868
PkrI	GCNGC	2 cut(s)	104, 1192
PleI	GAGTC	2 cut(s)	768, 1096
PmaCI	CACGTG	1 cut(s)	1445
PmlI	CACGTG	1 cut(s)	1445
PpsI	GAGTC	2 cut(s)	768, 1096
Ppu21I	YACGTR	1 cut(s)	1445
PpuMI	RGGWCCY	1 cut(s)	674
Psp1406I	AACGTT	1 cut(s)	1389
Psp5II	RGGWCCY	1 cut(s)	674
Psp6I	CCWGG	5 cut(s)	517, 727, 777, 868, 1122
PspCI	CACGTG	1 cut(s)	1445
PspGI	CCWGG	5 cut(s)	517, 727, 777, 868, 1122
PspN4I	GGNNCC	4 cut(s)	9, 675, 992, 1488
PspPI	GGNCC	7 cut(s)	8, 253, 552, 674, 863, 1120, 1528
PspPPI	RGGWCCY	1 cut(s)	674
PsuI	RGATCY	1 cut(s)	1486
RsaI	GTAC	4 cut(s)	56, 714, 1027, 1165
RsaNI	GTAC	4 cut(s)	55, 713, 1026, 1164
RseI	CAYNNNNRTG	1 cut(s)	83
SaqAI	TTAA	1 cut(s)	237
SatI	GCNGC	2 cut(s)	103, 1191
Sau96I	GGNCC	7 cut(s)	8, 253, 552, 674, 863, 1120, 1528
SchI	GAGTC	2 cut(s)	768, 1097
ScrFI	CCNGG	8 cut(s)	519, 582, 729, 779, 870, 1066, 1067, 1124
SduI	GDGCHC	1 cut(s)	490
SfaNI	GCATC	6 cut(s)	208, 509, 628, 798, 1082, 1390
SinI	GGWCC	6 cut(s)	8, 552, 674, 863, 1120, 1528
SmaI	CCCGGG	1 cut(s)	1067
SmiMI	CAYNNNNRTG	1 cut(s)	83
Sse9I	AATT	8 cut(s)	41, 276, 481, 1044, 1060, 1267, 1300, 1476
SseBI	AGGCCT	1 cut(s)	69
SsiI	CCGC	3 cut(s)	102, 470, 1534
SspI	AATATT	1 cut(s)	818
SspMI	CTAG	2 cut(s)	215, 612
StuI	AGGCCT	1 cut(s)	69
StyD4I	CCNGG	8 cut(s)	517, 580, 727, 777, 868, 1064, 1065, 1122
StyI	CCWWGG	4 cut(s)	60, 249, 839, 1205
TaaI	ACNGT	3 cut(s)	186, 565, 1312
TaiI	ACGT	3 cut(s)	982, 1392, 1447
TaqI	TCGA	2 cut(s)	96, 1523
TasI	AATT	8 cut(s)	41, 276, 481, 1044, 1060, 1267, 1300, 1476
TatI	WGTACW	2 cut(s)	712, 1025
TauI	GCSGC	1 cut(s)	105
TfiI	GAWTC	3 cut(s)	151, 623, 1049
Tru1I	TTAA	1 cut(s)	237
Tru9I	TTAA	1 cut(s)	237
TscAI	CASTG	1 cut(s)	189
TseFI	GTSAC	1 cut(s)	465
TseI	GCWGC	1 cut(s)	1190
Tsp45I	GTSAC	1 cut(s)	465
TspDTI	ATGAA	7 cut(s)	182, 269, 330, 1056, 1170, 1488, 1581
TspGWI	ACGGA	1 cut(s)	135
TspMI	CCCGGG	1 cut(s)	1065
TspRI	CASTG	1 cut(s)	189
VpaK11BI	GGWCC	6 cut(s)	8, 552, 674, 863, 1120, 1528
XapI	RAATTY	3 cut(s)	1267, 1300, 1476
XmaI	CCCGGG	1 cut(s)	1065
XmnI	GAANNNNTTC	1 cut(s)	819
XspI	CTAG	2 cut(s)	215, 612

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.