Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0015780

Physical Location & Seq

Genomic Coordinates Reverse (-)

28249410 .. 28252869

Gene Structure 3460 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g35820.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGGACTCCATGACCTCCTTTAAAGGCTACGGCAAAGTCGACGAGCTCGAAGAGCAAGCCTTCCGGCGCAAGACCCGAAAGCGCTTCATCATCGTCATCGTCTCCATCATTGTCCTCGTAGCCGTTATCATCGGCGCCGTCACCGGCACCGTCATCCACAACCGCAACAGCTCATCGTCGCCGTCACCAACCGAACCCGCCACCCAGTTGACTCCCTCCTCTTCTTTAAAAGCTGTCTGCGACGTCACCCTTTACCCCAACTCGTGTTTCTCGAGCATCTCTTCTCTCAAGAACTCCAACACCACCGACCCAGAAGCGCTCTTCAAGCTCTCCCTGCATGTCGCCATTGACGAGGTCTCGAAGCTCAAGGACTTGCCTTCGAAACTCAAGAGCAAGATCGGAAATGATACCAAGCTCAACGAGGCTTTCAAGGTCTGCGAGACCCTTTTCGACGACGCTGTGGATAAGCTTAACGACTCACTCTCTTCCATGGAGATCGGTTCCAGCGATAAGCTCTTGTCTCAGACGAAGATCAACGACTTAAAAACGTGGATCACCGCCTCCATGACTGACCAAGACACCTGCCTTGACTCTCTTGAAGAACTCAATTCAACTCTGGTTCAGGAATTCAGGGATGCTATGCAGAACTCGACGGAATACGCCAGCAACAGCCTGGCCATTGTGGCGAAAATTCTGGATTTACTGTCCAGAATGGGGATTCCGGTTCACCGGAGGTTACTCGGGTACGAAGAATCTTCCGTACCGGAGTTCCCGGGCTGGGTGAGCCCGGGAGACAGAAGGCTGCTTCAGGAGCAGAACCTGACTGCGCACGTGACCGTGGCGCAGGATGGTACCGGGGATTTTAAGACCATTCAAGAGGCTGTGAATGCTGTGCCCAAGAAGAACTTGACCAGGTTTGTGATATATGTGAAGCAAGGGACGTATTTGGAAAATGTGATTATGGACAAGAACAAATGGAATGTGATGATGTATGGAGATGGCAAGGCCAAGACCATTGTTTCTGGGAAGTTGAACTTTGTAGATGGGACTGCGACTTTCAACACTGCCACTTTTGCTGTTGCAGGAAAAGGGTTTATTGCGAAAGATATGGGGTTCATTAACACTGCTGGAGCGGCAAAGCACCAAGCAGTGGCATTCCGATCTGGTTCAGACCAATCTGTGTACTACAGATGCTCATTTGATGCATTCCAAGACACACTCTATGCTCACTCAAACCGACAATTCTATCGTGATTGTGACATCACAGGCACCATTGACTTCATCTTTGGCAATGCAGCCGTGGTGTTCCAGAACTGTAATATCCAGCCGAGGCAGCCAATGCCGAACCAGTTCAACACGATCACTGCTCAGGGGAAGAAGGACCCCAATCAGAACTCCGGGATTTCAATCCAAAAGTGCACAATCTTTCCACTCGAAAGCAATATGACAGCTCAAACATATCTTGGTAGACCTTGGAAGGAGTTTTCCACCACAATTTTCATGCAGTCCACTATTGGTTCAATCTTGAACCCATTGGGGTGGATAGAATGGGTTTCTAATGTTGATCCACCTAGCACCATATTTTACGCGGAGTATCAAAATAGTGGACCAGGATCAAGTGTGGATAAAAGGGTTACATGGGCTGGTTACAGGCCCTCTCTCACAGATGTTGAAGCAGCCAAGTTCACAGTTGAGTCCTTCATCCAAGGTAGTGAATGGTTGCCTCAGACTAATGTGAATTTTGAGACATCCCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0008150 GO:0009506 GO:0012505 GO:0016020 GO:0016787 GO:0016788 GO:0030054 GO:0030234 GO:0030599 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0043086 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044422 GO:0044424 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046910 GO:0050790 GO:0051722 GO:0051723 GO:0052689 GO:0055044 GO:0065007 GO:0065009 GO:0071944 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098805 GO:0140096

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

586

Amino Acids

64.71

Weight (kDa)

5.84

Isoelectric Point (pI)

32.77

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	75 - 227	1e-30	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	277 - 570	3.9e-140	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015780)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53840 AT3G14300
fragaria_vesca	FvH4_6g35820
malus_domestica	MD09G1172900.v1.1 MD17G1154400.v1.1
rosa_chinensis	RchiOBHm_Chr2g0147861
rosa_laevigata	RLG00000020286
rosa_multiflora	Rmu_sc0000308.1_g000054
rosa_roxburghii	Rroxscaffold_2G00099260
rosa_rugosa	Rorug02G0402500
rosa_samantha	Rh2AG459400 Rh2BG472800 Rh2CG446800 Rh2DG481700
rosa_wichuraiana	Rw2G037580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	590
AatII	GACGTC	1 cut(s)	246
Acc16I	TGCGCA	1 cut(s)	828
Acc36I	ACCTGC	1 cut(s)	590
Acc65I	GGTACC	1 cut(s)	851
AccB1I	GGYRCC	4 cut(s)	134, 146, 851, 1268
AccB7I	CCANNNNNTGG	1 cut(s)	1150
AccBSI	CCGCTC	1 cut(s)	1133
AccI	GTMKAC	2 cut(s)	39, 1468
AccII	CGCG	1 cut(s)	1589
AciI	CCGC	5 cut(s)	163, 198, 558, 1133, 1589
AclWI	GGATC	3 cut(s)	560, 1559, 1621
AcoI	YGGCCR	1 cut(s)	675
AcsI	RAATTY	3 cut(s)	626, 690, 1738
AcuI	CTGAAG	1 cut(s)	791
AcvI	CACGTG	1 cut(s)	832
AcyI	GRCGYC	2 cut(s)	135, 243
AfaI	GTAC	4 cut(s)	746, 762, 853, 1184
AfeI	AGCGCT	2 cut(s)	83, 318
AfiI	CCNNNNNNNGG	3 cut(s)	714, 778, 1150
AjnI	CCWGG	3 cut(s)	672, 911, 1609
AjuI	GAANNNNNNNTTGG	2 cut(s)	251, 283
AluBI	AGCT	9 cut(s)	46, 171, 233, 328, 364, 415, 469, 514, 1451
AluI	AGCT	9 cut(s)	46, 171, 233, 328, 364, 415, 469, 514, 1451
Alw21I	GWGCWC	2 cut(s)	48, 1421
Alw26I	GTCTC	6 cut(s)	106, 361, 434, 525, 786, 1739
Alw44I	GTGCAC	1 cut(s)	1417
AlwI	GGATC	3 cut(s)	560, 1559, 1621
AlwNI	CAGNNNCTG	1 cut(s)	820
Ama87I	CYCGRG	4 cut(s)	271, 740, 772, 787
Aor51HI	AGCGCT	2 cut(s)	83, 318
AoxI	GGCC	3 cut(s)	675, 1005, 1652
ApaLI	GTGCAC	1 cut(s)	1417
ApeKI	GCWGC	4 cut(s)	802, 1295, 1333, 1676
ApoI	RAATTY	3 cut(s)	626, 690, 1738
ArsI	GACNNNNNNTTYG	2 cut(s)	1268, 1300
Asp700I	GAANNNNTTC	1 cut(s)	1349
Asp718I	GGTACC	1 cut(s)	851
AspLEI	GCGC	6 cut(s)	69, 84, 137, 319, 829, 844
AspS9I	GGNCC	3 cut(s)	1381, 1607, 1653
AsuC2I	CCSGG	6 cut(s)	773, 774, 788, 789, 856, 1399
AsuHPI	GGTGA	6 cut(s)	133, 177, 238, 547, 719, 793
AsuII	TTCGAA	1 cut(s)	380
AvaI	CYCGRG	4 cut(s)	271, 740, 772, 787
AvaII	GGWCC	2 cut(s)	1381, 1607
BaeGI	GKGCMC	2 cut(s)	897, 1421
BalI	TGGCCA	1 cut(s)	677
BanI	GGYRCC	4 cut(s)	134, 146, 851, 1268
BanII	GRGCYC	2 cut(s)	48, 788
BauI	CACGAG	1 cut(s)	262
BbrPI	CACGTG	1 cut(s)	832
Bbv12I	GWGCWC	2 cut(s)	48, 1421
BbvI	GCAGC	4 cut(s)	789, 1307, 1345, 1688
BccI	CCATC	4 cut(s)	113, 842, 992, 1037
BceAI	ACGGC	5 cut(s)	46, 107, 122, 166, 1283
BciT130I	CCWGG	3 cut(s)	674, 913, 1611
BcnI	CCSGG	6 cut(s)	773, 774, 788, 789, 856, 1399
BcoDI	GTCTC	6 cut(s)	106, 361, 434, 525, 786, 1739
BfaI	CTAG	1 cut(s)	1572
BfmI	CTRYAG	1 cut(s)	1186
BfoI	RGCGCY	3 cut(s)	85, 138, 320
BfuAI	ACCTGC	1 cut(s)	590
BglI	GCCNNNNNGGC	1 cut(s)	683
BisI	GCNGC	5 cut(s)	803, 1134, 1296, 1334, 1677
BlsI	GCNGC	5 cut(s)	804, 1135, 1297, 1335, 1678
Bme1390I	CCNGG	9 cut(s)	674, 773, 774, 788, 789, 856, 913, 1399, 1611
Bme18I	GGWCC	2 cut(s)	1381, 1607
BmeT110I	CYCGRG	4 cut(s)	271, 740, 772, 787
BmgT120I	GGNCC	3 cut(s)	1381, 1607, 1653
BmiI	GGNNCC	6 cut(s)	136, 148, 502, 853, 1270, 1383
BmrFI	CCNGG	9 cut(s)	674, 773, 774, 788, 789, 856, 913, 1399, 1611
BmrI	ACTGGG	1 cut(s)	199
BmsI	GCATC	4 cut(s)	285, 625, 1181, 1192
BmuI	ACTGGG	1 cut(s)	199
BpmI	CTGGAG	1 cut(s)	1149
Bpu10I	CCTNAGC	1 cut(s)	1368
Bpu14I	TTCGAA	1 cut(s)	380
BpuEI	CTTGAG	3 cut(s)	272, 350, 371
BpuMI	CCSGG	6 cut(s)	773, 774, 788, 789, 856, 1399
BsaAI	YACGTR	1 cut(s)	832
BsaBI	GATNNNNATC	1 cut(s)	1406
BsaHI	GRCGYC	2 cut(s)	135, 243
BsaI	GGTCTC	2 cut(s)	361, 434
BsaJI	CCNNGG	9 cut(s)	489, 772, 787, 837, 855, 1299, 1328, 1472, 1705
BsaWI	WCCGGW	3 cut(s)	721, 729, 763
Bsc4I	CCNNNNNNNGG	3 cut(s)	714, 778, 1150
Bse118I	RCCGGY	1 cut(s)	143
Bse1I	ACTGG	2 cut(s)	205, 1348
Bse3DI	GCAATG	1 cut(s)	1297
Bse8I	GATNNNNATC	1 cut(s)	1406
BseBI	CCWGG	3 cut(s)	674, 913, 1611
BseDI	CCNNGG	9 cut(s)	489, 772, 787, 837, 855, 1299, 1328, 1472, 1705
BseGI	GGATG	5 cut(s)	153, 640, 853, 1701, 1748
BseJI	GATNNNNATC	1 cut(s)	1406
BseLI	CCNNNNNNNGG	3 cut(s)	714, 778, 1150
BseMI	GCAATG	1 cut(s)	1297
BseMII	CTCAG	3 cut(s)	536, 1382, 1739
BseNI	ACTGG	2 cut(s)	205, 1348
BseRI	GAGGAG	1 cut(s)	208
BseSI	GKGCMC	2 cut(s)	897, 1421
BseXI	GCAGC	4 cut(s)	789, 1307, 1345, 1688
BseYI	CCCAGC	1 cut(s)	777
Bsh1236I	CGCG	1 cut(s)	1589
BshFI	GGCC	3 cut(s)	677, 1007, 1654
BshNI	GGYRCC	4 cut(s)	134, 146, 851, 1268
BsiHKAI	GWGCWC	2 cut(s)	48, 1421
BsiHKCI	CYCGRG	4 cut(s)	271, 740, 772, 787
BsiSI	CCGG	9 cut(s)	64, 144, 722, 730, 764, 773, 788, 855, 1398
BslFI	GGGAC	2 cut(s)	952, 1060
BslI	CCNNNNNNNGG	3 cut(s)	714, 778, 1150
BsmAI	GTCTC	6 cut(s)	106, 361, 434, 525, 786, 1739
BsmBI	CGTCTC	1 cut(s)	106
BsmFI	GGGAC	2 cut(s)	952, 1060
BsmI	GAATGC	3 cut(s)	892, 1154, 1205
BsnI	GGCC	3 cut(s)	677, 1007, 1654
Bso31I	GGTCTC	2 cut(s)	361, 434
BsoBI	CYCGRG	4 cut(s)	271, 740, 772, 787
Bsp119I	TTCGAA	1 cut(s)	380
Bsp1286I	GDGCHC	4 cut(s)	48, 788, 897, 1421
Bsp143I	GATC	8 cut(s)	396, 495, 531, 552, 1160, 1359, 1564, 1613
Bsp19I	CCATGG	1 cut(s)	489
BspACI	CCGC	5 cut(s)	163, 198, 558, 1133, 1589
BspANI	GGCC	3 cut(s)	677, 1007, 1654
BspCNI	CTCAG	3 cut(s)	535, 1381, 1738
BspFNI	CGCG	1 cut(s)	1589
BspLI	GGNNCC	6 cut(s)	136, 148, 502, 853, 1270, 1383
BspMI	ACCTGC	1 cut(s)	590
BspPI	GGATC	3 cut(s)	560, 1559, 1621
BspQI	GCTCTTC	2 cut(s)	45, 326
BspT104I	TTCGAA	1 cut(s)	380
BspT107I	GGYRCC	4 cut(s)	134, 146, 851, 1268
BspTNI	GGTCTC	2 cut(s)	361, 434
BsrBI	CCGCTC	1 cut(s)	1133
BsrDI	GCAATG	1 cut(s)	1297
BsrFI	RCCGGY	1 cut(s)	143
BsrI	ACTGG	2 cut(s)	205, 1348
BssAI	RCCGGY	1 cut(s)	143
BssECI	CCNNGG	9 cut(s)	489, 772, 787, 837, 855, 1299, 1328, 1472, 1705
BssMI	GATC	8 cut(s)	396, 495, 531, 552, 1160, 1359, 1564, 1613
BssNI	GRCGYC	2 cut(s)	135, 243
BssSI	CACGAG	1 cut(s)	262
BssT1I	CCWWGG	3 cut(s)	489, 1472, 1705
Bst2BI	CACGAG	1 cut(s)	262
Bst2UI	CCWGG	3 cut(s)	674, 913, 1611
Bst4CI	ACNGT	5 cut(s)	151, 705, 838, 1316, 1690
Bst6I	CTCTTC	5 cut(s)	45, 226, 286, 326, 490
BstACI	GRCGYC	2 cut(s)	135, 243
BstAPI	GCANNNNNTGC	1 cut(s)	1339
BstBAI	YACGTR	1 cut(s)	832
BstBI	TTCGAA	1 cut(s)	380
BstC8I	GCNNGC	2 cut(s)	57, 664
BstDEI	CTNAG	3 cut(s)	522, 1368, 1725
BstDSI	CCRYGG	3 cut(s)	489, 837, 1299
BstF5I	GGATG	5 cut(s)	153, 640, 853, 1701, 1748
BstFNI	CGCG	1 cut(s)	1589
BstH2I	RGCGCY	3 cut(s)	85, 138, 320
BstHHI	GCGC	6 cut(s)	69, 84, 137, 319, 829, 844
BstKTI	GATC	8 cut(s)	399, 498, 534, 555, 1163, 1362, 1567, 1616
BstMAI	GTCTC	6 cut(s)	106, 361, 434, 525, 786, 1739
BstMBI	GATC	8 cut(s)	396, 495, 531, 552, 1160, 1359, 1564, 1613
BstNI	CCWGG	3 cut(s)	674, 913, 1611
BstNSI	RCATGY	1 cut(s)	341
BstSCI	CCNGG	9 cut(s)	672, 771, 772, 786, 787, 854, 911, 1397, 1609
BstSFI	CTRYAG	1 cut(s)	1186
BstSLI	GKGCMC	2 cut(s)	897, 1421
BstUI	CGCG	1 cut(s)	1589
BstV1I	GCAGC	4 cut(s)	789, 1307, 1345, 1688
BstXI	CCANNNNNNTGG	1 cut(s)	1539
BsuRI	GGCC	3 cut(s)	677, 1007, 1654
BtgI	CCRYGG	3 cut(s)	489, 837, 1299
BtsCI	GGATG	5 cut(s)	153, 640, 853, 1701, 1748
BtsI	GCAGTG	4 cut(s)	1062, 1122, 1155, 1362
BtsIMutI	CAGTG	4 cut(s)	1062, 1122, 1155, 1362
BveI	ACCTGC	1 cut(s)	590
Cac8I	GCNNGC	2 cut(s)	57, 664
CaiI	CAGNNNCTG	1 cut(s)	820
CfoI	GCGC	6 cut(s)	69, 84, 137, 319, 829, 844
Cfr10I	RCCGGY	1 cut(s)	143
Cfr13I	GGNCC	3 cut(s)	1381, 1607, 1653
Cfr9I	CCCGGG	2 cut(s)	772, 787
CseI	GACGC	1 cut(s)	464
CsiI	ACCWGGT	1 cut(s)	911
Csp6I	GTAC	4 cut(s)	745, 761, 852, 1183
CviAII	CATG	6 cut(s)	10, 338, 490, 565, 1501, 1638
CviQI	GTAC	4 cut(s)	745, 761, 852, 1183
DdeI	CTNAG	3 cut(s)	522, 1368, 1725
DinI	GGCGCC	1 cut(s)	136
DpnI	GATC	8 cut(s)	398, 497, 533, 554, 1162, 1361, 1566, 1615
DpnII	GATC	8 cut(s)	396, 495, 531, 552, 1160, 1359, 1564, 1613
DraI	TTTAAA	2 cut(s)	22, 228
EaeI	YGGCCR	1 cut(s)	675
Eam1104I	CTCTTC	5 cut(s)	45, 226, 286, 326, 490
EarI	CTCTTC	5 cut(s)	45, 226, 286, 326, 490
Ecl136II	GAGCTC	1 cut(s)	46
Eco130I	CCWWGG	3 cut(s)	489, 1472, 1705
Eco24I	GRGCYC	2 cut(s)	48, 788
Eco31I	GGTCTC	2 cut(s)	361, 434
Eco47I	GGWCC	2 cut(s)	1381, 1607
Eco47III	AGCGCT	2 cut(s)	83, 318
Eco53kI	GAGCTC	1 cut(s)	46
Eco57I	CTGAAG	1 cut(s)	791
Eco72I	CACGTG	1 cut(s)	832
Eco88I	CYCGRG	4 cut(s)	271, 740, 772, 787
EcoICRI	GAGCTC	1 cut(s)	46
EcoO109I	RGGNCCY	2 cut(s)	1381, 1653
EcoRI	GAATTC	1 cut(s)	626
EcoRII	CCWGG	3 cut(s)	672, 911, 1609
EcoT14I	CCWWGG	3 cut(s)	489, 1472, 1705
EcoT22I	ATGCAT	1 cut(s)	1207
EcoT38I	GRGCYC	2 cut(s)	48, 788
EgeI	GGCGCC	1 cut(s)	136
EheI	GGCGCC	1 cut(s)	136
ErhI	CCWWGG	3 cut(s)	489, 1472, 1705
Esp3I	CGTCTC	1 cut(s)	106
FaeI	CATG	6 cut(s)	13, 341, 493, 568, 1504, 1641
FalI	AAGNNNNNCTT	4 cut(s)	890, 922, 1019, 1051
FaqI	GGGAC	2 cut(s)	952, 1060
FatI	CATG	6 cut(s)	9, 337, 489, 564, 1500, 1637
FauI	CCCGC	1 cut(s)	205
FblI	GTMKAC	2 cut(s)	39, 1468
Fnu4HI	GCNGC	5 cut(s)	803, 1134, 1296, 1334, 1677
FokI	GGATG	5 cut(s)	140, 647, 860, 1688, 1735
FriOI	GRGCYC	2 cut(s)	48, 788
Fsp4HI	GCNGC	5 cut(s)	803, 1134, 1296, 1334, 1677
FspBI	CTAG	1 cut(s)	1572
FspI	TGCGCA	1 cut(s)	828
GlaI	GCGC	6 cut(s)	68, 83, 136, 318, 828, 843
GluI	GCNGC	5 cut(s)	803, 1134, 1296, 1334, 1677
GsaI	CCCAGC	1 cut(s)	781
GsuI	CTGGAG	1 cut(s)	1149
HaeII	RGCGCY	3 cut(s)	85, 138, 320
HaeIII	GGCC	3 cut(s)	677, 1007, 1654
HapII	CCGG	9 cut(s)	64, 144, 722, 730, 764, 773, 788, 855, 1398
HgaI	GACGC	1 cut(s)	464
HhaI	GCGC	6 cut(s)	69, 84, 137, 319, 829, 844
Hin1I	GRCGYC	2 cut(s)	135, 243
Hin1II	CATG	6 cut(s)	13, 341, 493, 568, 1504, 1641
Hin6I	GCGC	6 cut(s)	67, 82, 135, 317, 827, 842
HinP1I	GCGC	6 cut(s)	67, 82, 135, 317, 827, 842
HincII	GTYRAC	2 cut(s)	40, 210
HindII	GTYRAC	2 cut(s)	40, 210
HindIII	AAGCTT	1 cut(s)	467
HinfI	GANTC	7 cut(s)	5, 211, 476, 590, 718, 752, 1694
HpaII	CCGG	9 cut(s)	64, 144, 722, 730, 764, 773, 788, 855, 1398
HphI	GGTGA	6 cut(s)	133, 177, 238, 547, 719, 793
Hpy166II	GTNNAC	9 cut(s)	40, 210, 727, 1183, 1419, 1469, 1509, 1607, 1686
Hpy188I	TCNGA	6 cut(s)	401, 525, 1160, 1171, 1392, 1728
Hpy8I	GTNNAC	9 cut(s)	40, 210, 727, 1183, 1419, 1469, 1509, 1607, 1686
Hpy99I	CGWCG	6 cut(s)	44, 181, 245, 455, 458, 655
HpyAV	CCTTC	6 cut(s)	70, 387, 792, 1372, 1471, 1708
HpyCH4III	ACNGT	5 cut(s)	151, 705, 838, 1316, 1690
HpyCH4IV	ACGT	4 cut(s)	243, 548, 831, 941
HpyCH4V	TGCA	7 cut(s)	337, 643, 1082, 1205, 1295, 1419, 1504
HpyF3I	CTNAG	3 cut(s)	522, 1368, 1725
HpySE526I	ACGT	4 cut(s)	243, 548, 831, 941
Hsp92I	GRCGYC	2 cut(s)	135, 243
Hsp92II	CATG	6 cut(s)	13, 341, 493, 568, 1504, 1641
HspAI	GCGC	6 cut(s)	67, 82, 135, 317, 827, 842
KasI	GGCGCC	1 cut(s)	134
KpnI	GGTACC	1 cut(s)	855
Kzo9I	GATC	8 cut(s)	396, 495, 531, 552, 1160, 1359, 1564, 1613
LguI	GCTCTTC	2 cut(s)	45, 326
LmnI	GCTCC	2 cut(s)	811, 1130
Lsp1109I	GCAGC	4 cut(s)	789, 1307, 1345, 1688
LweI	GCATC	4 cut(s)	285, 625, 1181, 1192
MabI	ACCWGGT	1 cut(s)	911
MaeI	CTAG	1 cut(s)	1572
MaeII	ACGT	4 cut(s)	243, 548, 831, 941
MaeIII	GTNAC	8 cut(s)	139, 183, 244, 735, 832, 1256, 1633, 1646
MalI	GATC	8 cut(s)	398, 497, 533, 554, 1162, 1361, 1566, 1615
MbiI	CCGCTC	1 cut(s)	1133
MboI	GATC	8 cut(s)	396, 495, 531, 552, 1160, 1359, 1564, 1613
MhlI	GDGCHC	4 cut(s)	48, 788, 897, 1421
MlsI	TGGCCA	1 cut(s)	677
MluCI	AATT	6 cut(s)	607, 626, 690, 1241, 1494, 1738
MluNI	TGGCCA	1 cut(s)	677
Mly113I	GGCGCC	1 cut(s)	135
MlyI	GAGTC	4 cut(s)	205, 470, 584, 1703
MmeI	TCCRAC	1 cut(s)	321
Mox20I	TGGCCA	1 cut(s)	677
Mph1103I	ATGCAT	1 cut(s)	1207
MroXI	GAANNNNTTC	1 cut(s)	1349
MscI	TGGCCA	1 cut(s)	677
MseI	TTAA	6 cut(s)	21, 227, 471, 542, 864, 1119
MslI	CAYNNNNRTG	1 cut(s)	1537
Msp20I	TGGCCA	1 cut(s)	677
MspI	CCGG	9 cut(s)	64, 144, 722, 730, 764, 773, 788, 855, 1398
MspR9I	CCNGG	9 cut(s)	674, 773, 774, 788, 789, 856, 913, 1399, 1611
Mva1269I	GAATGC	3 cut(s)	892, 1154, 1205
MvaI	CCWGG	3 cut(s)	674, 913, 1611
MvnI	CGCG	1 cut(s)	1589
NarI	GGCGCC	1 cut(s)	135
NciI	CCSGG	6 cut(s)	773, 774, 788, 789, 856, 1399
NcoI	CCATGG	1 cut(s)	489
NdeII	GATC	8 cut(s)	396, 495, 531, 552, 1160, 1359, 1564, 1613
NlaIII	CATG	6 cut(s)	13, 341, 493, 568, 1504, 1641
NlaIV	GGNNCC	6 cut(s)	136, 148, 502, 853, 1270, 1383
NmeAIII	GCCGAG	1 cut(s)	1353
NmuCI	GTSAC	5 cut(s)	139, 183, 244, 832, 1256
NsbI	TGCGCA	1 cut(s)	828
NsiI	ATGCAT	1 cut(s)	1207
NspI	RCATGY	1 cut(s)	341
NspV	TTCGAA	1 cut(s)	380
PaeR7I	CTCGAG	1 cut(s)	271
PaqCI	CACCTGC	1 cut(s)	590
PciSI	GCTCTTC	2 cut(s)	45, 326
PcsI	WCGNNNNNNNCGW	5 cut(s)	36, 45, 269, 348, 504
PctI	GAATGC	3 cut(s)	892, 1154, 1205
PdmI	GAANNNNTTC	1 cut(s)	1349
PfeI	GAWTC	2 cut(s)	718, 752
PflFI	GACNNNGTC	1 cut(s)	353
PflMI	CCANNNNNTGG	1 cut(s)	1150
PfoI	TCCNGGA	1 cut(s)	1397
PkrI	GCNGC	5 cut(s)	804, 1135, 1297, 1335, 1678
PleI	GAGTC	4 cut(s)	205, 470, 584, 1702
PluTI	GGCGCC	1 cut(s)	138
PmaCI	CACGTG	1 cut(s)	832
PmlI	CACGTG	1 cut(s)	832
PpsI	GAGTC	4 cut(s)	205, 470, 584, 1702
Ppu21I	YACGTR	1 cut(s)	832
PpuMI	RGGWCCY	1 cut(s)	1381
Psp124BI	GAGCTC	1 cut(s)	48
Psp5II	RGGWCCY	1 cut(s)	1381
Psp6I	CCWGG	3 cut(s)	672, 911, 1609
PspCI	CACGTG	1 cut(s)	832
PspFI	CCCAGC	1 cut(s)	777
PspGI	CCWGG	3 cut(s)	672, 911, 1609
PspN4I	GGNNCC	6 cut(s)	136, 148, 502, 853, 1270, 1383
PspPI	GGNCC	3 cut(s)	1381, 1607, 1653
PspPPI	RGGWCCY	1 cut(s)	1381
PstNI	CAGNNNCTG	1 cut(s)	820
PsyI	GACNNNGTC	1 cut(s)	353
RsaI	GTAC	4 cut(s)	746, 762, 853, 1184
RsaNI	GTAC	4 cut(s)	745, 761, 852, 1183
RseI	CAYNNNNRTG	1 cut(s)	1537
SacI	GAGCTC	1 cut(s)	48
SalI	GTCGAC	1 cut(s)	38
SapI	GCTCTTC	2 cut(s)	45, 326
SaqAI	TTAA	6 cut(s)	21, 227, 471, 542, 864, 1119
SatI	GCNGC	5 cut(s)	803, 1134, 1296, 1334, 1677
Sau3AI	GATC	8 cut(s)	396, 495, 531, 552, 1160, 1359, 1564, 1613
Sau96I	GGNCC	3 cut(s)	1381, 1607, 1653
SchI	GAGTC	4 cut(s)	205, 470, 584, 1703
ScrFI	CCNGG	9 cut(s)	674, 773, 774, 788, 789, 856, 913, 1399, 1611
SduI	GDGCHC	4 cut(s)	48, 788, 897, 1421
SexAI	ACCWGGT	1 cut(s)	911
SfaNI	GCATC	4 cut(s)	285, 625, 1181, 1192
SfcI	CTRYAG	1 cut(s)	1186
SfoI	GGCGCC	1 cut(s)	136
Sfr274I	CTCGAG	1 cut(s)	271
SfuI	TTCGAA	1 cut(s)	380
SinI	GGWCC	2 cut(s)	1381, 1607
SlaI	CTCGAG	1 cut(s)	271
SmaI	CCCGGG	2 cut(s)	774, 789
SmiMI	CAYNNNNRTG	1 cut(s)	1537
SmlI	CTYRAG	4 cut(s)	271, 287, 365, 386
SmoI	CTYRAG	4 cut(s)	271, 287, 365, 386
Sse9I	AATT	6 cut(s)	607, 626, 690, 1241, 1494, 1738
SsiI	CCGC	5 cut(s)	163, 198, 558, 1133, 1589
SspDI	GGCGCC	1 cut(s)	134
SspMI	CTAG	1 cut(s)	1572
SstI	GAGCTC	1 cut(s)	48
StyD4I	CCNGG	9 cut(s)	672, 771, 772, 786, 787, 854, 911, 1397, 1609
StyI	CCWWGG	3 cut(s)	489, 1472, 1705
TaaI	ACNGT	5 cut(s)	151, 705, 838, 1316, 1690
TaiI	ACGT	4 cut(s)	246, 551, 834, 944
TaqI	TCGA	8 cut(s)	39, 48, 272, 359, 380, 450, 650, 1434
TasI	AATT	6 cut(s)	607, 626, 690, 1241, 1494, 1738
TatI	WGTACW	1 cut(s)	1182
TauI	GCSGC	1 cut(s)	1136
TfiI	GAWTC	2 cut(s)	718, 752
Tru1I	TTAA	6 cut(s)	21, 227, 471, 542, 864, 1119
Tru9I	TTAA	6 cut(s)	21, 227, 471, 542, 864, 1119
TscAI	CASTG	4 cut(s)	1069, 1129, 1155, 1369
TseFI	GTSAC	5 cut(s)	139, 183, 244, 832, 1256
TseI	GCWGC	4 cut(s)	802, 1295, 1333, 1676
Tsp45I	GTSAC	5 cut(s)	139, 183, 244, 832, 1256
TspDTI	ATGAA	5 cut(s)	76, 1105, 1270, 1489, 1690
TspGWI	ACGGA	2 cut(s)	668, 748
TspMI	CCCGGG	2 cut(s)	772, 787
TspRI	CASTG	4 cut(s)	1069, 1129, 1155, 1369
Tth111I	GACNNNGTC	1 cut(s)	353
Van91I	CCANNNNNTGG	1 cut(s)	1150
VneI	GTGCAC	1 cut(s)	1417
VpaK11BI	GGWCC	2 cut(s)	1381, 1607
XapI	RAATTY	3 cut(s)	626, 690, 1738
XceI	RCATGY	1 cut(s)	341
XcmI	CCANNNNNNNNNTGG	1 cut(s)	670
XhoI	CTCGAG	1 cut(s)	271
XmaI	CCCGGG	2 cut(s)	772, 787
XmiI	GTMKAC	2 cut(s)	39, 1468
XmnI	GAANNNNTTC	1 cut(s)	1349
XspI	CTAG	1 cut(s)	1572
ZraI	GACGTC	1 cut(s)	244
Zsp2I	ATGCAT	1 cut(s)	1207

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.