Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000002

Orthogroup ID

OG0015780

Physical Location & Seq

Genomic Coordinates Forward (+)

20895715 .. 20898890

Gene Structure 3176 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_2G00099260.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGACCTCCTTCAAGGGCTACGGCAAAGTCGACGAGCTCGAAGCGCAAGCCTACCGTCTCAAAACCCGCAAGCGCATGCTCATCGTCACCGTCTCCGTCATTGTCCTCTTGGCCGTCATCATCGGCGCCGTTACCGGCACCGTCATCCACAACCGCAACAGCTCCTCTTCGCCGTCGCCGAACACGGAACCCGCCACCCAGTTGACTCCCTCCGCTTCCTTAAAAGCCGTCTGCGGCGTCACCCAGTACCCCAACTCGTGTTTCTCGAGCATCTCTTCTCTCAAAAATTCCAACACCACCGACCCGGAAGCGCTGTTCAAGCTGTCCCTGCATGTCGCCATTAACGAGGCCTCGAAACTCAAAGACTTGCCTGCAACAATAAAGAGTAAGATCAGAAATGATACCAAACTCGAAAGCGCTATTAAGGTCTGCGAGACCGTTTTCGATGATGCTGTGGATAAGCTTAACGATTCGCTCTCTGCAATGGATGTCAGTTCCGGCGAGAAGCTGTTGTCCGAGGCGAAGATCAATGACCTGAAGACGTGGATCAGCGCCACCATGACTGACCAAGAGACCTGCCTGGACTCTCTTGAAGACATCAATTCGACTTATGTTCAGGAATTCAGGGATGCGATGCAGAACTCCACAGAATACGCCAGCAACAGCCTGGCCATTGTGGCGAAAATTCTGGGGTTATTGTCCAATTTGAACATTCCGGTTCACCGGAGATTGCTCGGATACGGAGGAGAAAGATCCTCCGTACCCGAGTTCCCGGGCTGGGTCAGCCCGGGAGACAGGAGGCTGCTGCAGGAGAAGAACCTGACCGCGCACGTGACTGTGGCGAAGGATGGGACTGGGGATTTTAAGACCATTCAGGAGGCTGTGAATGCTGTGCCCAAGAAGAACTTGACCAGGTTTGTGATATATGTGAAGCAAGGGACGTACGAAGAAAATGTGATCATGGACAAGAGCAAATGGAATGTGATGATGTATGGAGATGACAAGACCAAGACCATTGTTTCTGGGAAGTTGAACTATGTGGATGGGATTGCAACTTTCAACACAGCCACTTTTGCTGTAGCAGGAAGAGGGTTCATTGCGAAAGATATAGGCTTCATTAACACTGCTGGAGCTGCAAAGCACCAAGCGGTTGCATTCCGATCTGGTTCCGACCAATCTGTGTTCTACAGATGCTCATTTGATGCATTCCAGGACACTCTATATGCTCACTCGAATCGACAATTCTATCGTGACTGTGACATCACTGGTACCATTGACTTCATGTTTGGCAATGCAGCTGTGGTGTTCCAGAACTGTAACATCAAGCCGAGGCAACCAATGCCAAACCAGTTCAACACAATCACTGCTCAGGGAAAGACGGACCCCAATCAAAACACTGGCATTTCAATTCAGAAGTGCACAATCTCTCCACTCGAAAGCAACATGACGGCTCCAACATATCTTGGTAGACCTTGGAAGAACTTTTCCACCACGGTTTTCTTACAATCCAGTATTGATTCGTTCTTGAACCCATCGGGGTGGACAGAATGGACTCCCCATGTTGATCCACCTAACACCATATTTTACGCAGAATACCAAAATAGTGGACCAGGATCGAGTGTGGATAAAAGGGTTGCATGGGCTGGTTACAGGCCCTCTCTCACAGATACTGAAGCAGCCAAGTTCACAGTTGAGTCCTTCATCCAAGGTAATGAATGGTTGCCTCAGACTAATGTGAATTTTGAGTCATCCCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0008150 GO:0009506 GO:0012505 GO:0016020 GO:0016787 GO:0016788 GO:0030054 GO:0030234 GO:0030599 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0043086 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044422 GO:0044424 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046910 GO:0050790 GO:0051722 GO:0051723 GO:0052689 GO:0055044 GO:0065007 GO:0065009 GO:0071944 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098805 GO:0140096

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

585

Amino Acids

64.39

Weight (kDa)

6.68

Isoelectric Point (pI)

30.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	73 - 225	1.2e-30	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	277 - 571	1.4e-139	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015780)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53840 AT3G14300
fragaria_vesca	FvH4_6g35820
malus_domestica	MD09G1172900.v1.1 MD17G1154400.v1.1
rosa_chinensis	RchiOBHm_Chr2g0147861
rosa_laevigata	RLG00000020286
rosa_multiflora	Rmu_sc0000308.1_g000054
rosa_roxburghii	Rroxscaffold_2G00099260
rosa_rugosa	Rorug02G0402500
rosa_samantha	Rh2AG459400 Rh2BG472800 Rh2CG446800 Rh2DG481700
rosa_wichuraiana	Rw2G037580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	584
Acc65I	GGTACC	1 cut(s)	1268
AccB1I	GGYRCC	3 cut(s)	125, 137, 1268
AccI	GTMKAC	2 cut(s)	30, 1468
AccII	CGCG	1 cut(s)	827
AciI	CCGC	7 cut(s)	67, 154, 192, 213, 234, 825, 1148
AclWI	GGATC	4 cut(s)	554, 747, 1559, 1621
AcoI	YGGCCR	2 cut(s)	111, 669
AcsI	RAATTY	4 cut(s)	286, 620, 684, 1738
AcuI	CTGAAG	2 cut(s)	557, 1692
AcvI	CACGTG	1 cut(s)	832
AcyI	GRCGYC	2 cut(s)	126, 237
AfaI	GTAC	4 cut(s)	248, 762, 944, 1270
AfeI	AGCGCT	2 cut(s)	312, 418
AfiI	CCNNNNNNNGG	1 cut(s)	778
AgsI	TTSAA	9 cut(s)	13, 319, 593, 709, 1033, 1060, 1354, 1407, 1528
AjiI	CACGTC	1 cut(s)	543
AjnI	CCWGG	5 cut(s)	579, 666, 911, 1209, 1609
AjuI	GAANNNNNNNTTGG	2 cut(s)	245, 277
AloI	GAACNNNNNNTCC	2 cut(s)	299, 331
AluBI	AGCT	7 cut(s)	37, 162, 322, 463, 508, 1133, 1298
AluI	AGCT	7 cut(s)	37, 162, 322, 463, 508, 1133, 1298
Alw21I	GWGCWC	2 cut(s)	39, 1421
Alw26I	GTCTC	5 cut(s)	62, 97, 428, 566, 786
Alw44I	GTGCAC	1 cut(s)	1417
AlwI	GGATC	4 cut(s)	554, 747, 1559, 1621
AlwNI	CAGNNNCTG	1 cut(s)	1670
Ama87I	CYCGRG	4 cut(s)	265, 764, 772, 787
Aor51HI	AGCGCT	2 cut(s)	312, 418
AoxI	GGCC	4 cut(s)	111, 348, 669, 1652
ApaLI	GTGCAC	1 cut(s)	1417
ApeKI	GCWGC	5 cut(s)	802, 805, 1133, 1295, 1676
ApoI	RAATTY	4 cut(s)	286, 620, 684, 1738
ArsI	GACNNNNNNTTYG	2 cut(s)	1268, 1300
Asp718I	GGTACC	1 cut(s)	1268
AspLEI	GCGC	7 cut(s)	46, 75, 128, 313, 419, 554, 829
AspS9I	GGNCC	3 cut(s)	1381, 1607, 1653
AsuC2I	CCSGG	5 cut(s)	305, 773, 774, 788, 789
AsuHPI	GGTGA	3 cut(s)	79, 232, 713
AvaI	CYCGRG	4 cut(s)	265, 764, 772, 787
AvaII	GGWCC	2 cut(s)	1381, 1607
BaeGI	GKGCMC	2 cut(s)	897, 1421
BalI	TGGCCA	1 cut(s)	671
BanI	GGYRCC	3 cut(s)	125, 137, 1268
BanII	GRGCYC	1 cut(s)	39
BauI	CACGAG	1 cut(s)	256
BbrPI	CACGTG	1 cut(s)	832
BbsI	GAAGAC	2 cut(s)	545, 600
Bbv12I	GWGCWC	2 cut(s)	39, 1421
BbvI	GCAGC	5 cut(s)	789, 792, 1120, 1307, 1688
BccI	CCATC	3 cut(s)	842, 1037, 1540
BceAI	ACGGC	6 cut(s)	37, 98, 113, 157, 212, 1464
BcgI	CGANNNNNNTGC	2 cut(s)	64, 98
BciT130I	CCWGG	5 cut(s)	581, 668, 913, 1211, 1611
BciVI	GTATCC	1 cut(s)	731
BclI	TGATCA	1 cut(s)	957
BcnI	CCSGG	5 cut(s)	305, 773, 774, 788, 789
BcoDI	GTCTC	5 cut(s)	62, 97, 428, 566, 786
BfmI	CTRYAG	3 cut(s)	806, 1077, 1186
BfoI	RGCGCY	4 cut(s)	129, 314, 420, 555
BfuAI	ACCTGC	1 cut(s)	584
BfuI	GTATCC	1 cut(s)	731
BglI	GCCNNNNNGGC	1 cut(s)	677
BisI	GCNGC	6 cut(s)	235, 803, 806, 1134, 1296, 1677
BlsI	GCNGC	6 cut(s)	236, 804, 807, 1135, 1297, 1678
Bme18I	GGWCC	2 cut(s)	1381, 1607
BmeT110I	CYCGRG	4 cut(s)	265, 764, 772, 787
BmgBI	CACGTC	1 cut(s)	543
BmgT120I	GGNCC	3 cut(s)	1381, 1607, 1653
BmiI	GGNNCC	7 cut(s)	127, 139, 189, 1168, 1270, 1383, 1452
BmrI	ACTGGG	3 cut(s)	193, 238, 864
BmsI	GCATC	6 cut(s)	279, 439, 619, 624, 1181, 1192
BmuI	ACTGGG	3 cut(s)	193, 238, 864
BpiI	GAAGAC	2 cut(s)	545, 600
BpmI	CTGGAG	1 cut(s)	1149
Bpu10I	CCTNAGC	1 cut(s)	1368
BpuMI	CCSGG	5 cut(s)	305, 773, 774, 788, 789
BsaAI	YACGTR	1 cut(s)	832
BsaHI	GRCGYC	2 cut(s)	126, 237
BsaI	GGTCTC	2 cut(s)	428, 566
BsaJI	CCNNGG	7 cut(s)	516, 772, 787, 1328, 1472, 1491, 1705
BsaWI	WCCGGW	2 cut(s)	715, 723
BsaXI	ACNNNNNCTCC	2 cut(s)	1411, 1441
Bsc4I	CCNNNNNNNGG	1 cut(s)	778
Bse118I	RCCGGY	1 cut(s)	134
Bse1I	ACTGG	7 cut(s)	199, 244, 859, 1270, 1348, 1402, 1509
Bse3DI	GCAATG	3 cut(s)	489, 1095, 1297
BseBI	CCWGG	5 cut(s)	581, 668, 913, 1211, 1611
BseDI	CCNNGG	7 cut(s)	516, 772, 787, 1328, 1472, 1491, 1705
BseGI	GGATG	7 cut(s)	144, 493, 634, 853, 1048, 1701, 1748
BseLI	CCNNNNNNNGG	1 cut(s)	778
BseMI	GCAATG	3 cut(s)	489, 1095, 1297
BseMII	CTCAG	2 cut(s)	1382, 1739
BseNI	ACTGG	7 cut(s)	199, 244, 859, 1270, 1348, 1402, 1509
BseRI	GAGGAG	2 cut(s)	154, 759
BseSI	GKGCMC	2 cut(s)	897, 1421
BseXI	GCAGC	5 cut(s)	789, 792, 1120, 1307, 1688
BseYI	CCCAGC	1 cut(s)	777
Bsh1236I	CGCG	1 cut(s)	827
BshFI	GGCC	4 cut(s)	113, 350, 671, 1654
BshNI	GGYRCC	3 cut(s)	125, 137, 1268
BsiHKAI	GWGCWC	2 cut(s)	39, 1421
BsiHKCI	CYCGRG	4 cut(s)	265, 764, 772, 787
BsiSI	CCGG	7 cut(s)	135, 305, 498, 716, 724, 773, 788
BsiWI	CGTACG	1 cut(s)	942
BslFI	GGGAC	3 cut(s)	310, 865, 952
BslI	CCNNNNNNNGG	1 cut(s)	778
BsmAI	GTCTC	5 cut(s)	62, 97, 428, 566, 786
BsmBI	CGTCTC	2 cut(s)	62, 97
BsmFI	GGGAC	3 cut(s)	310, 865, 952
BsmI	GAATGC	3 cut(s)	892, 1154, 1205
BsnI	GGCC	4 cut(s)	113, 350, 671, 1654
Bso31I	GGTCTC	2 cut(s)	428, 566
BsoBI	CYCGRG	4 cut(s)	265, 764, 772, 787
Bsp1286I	GDGCHC	3 cut(s)	39, 897, 1421
Bsp143I	GATC	8 cut(s)	390, 525, 546, 752, 957, 1160, 1564, 1613
BspACI	CCGC	7 cut(s)	67, 154, 192, 213, 234, 825, 1148
BspANI	GGCC	4 cut(s)	113, 350, 671, 1654
BspCNI	CTCAG	2 cut(s)	1381, 1738
BspFNI	CGCG	1 cut(s)	827
BspLI	GGNNCC	7 cut(s)	127, 139, 189, 1168, 1270, 1383, 1452
BspMAI	CTGCAG	1 cut(s)	810
BspMI	ACCTGC	1 cut(s)	584
BspPI	GGATC	4 cut(s)	554, 747, 1559, 1621
BspT107I	GGYRCC	3 cut(s)	125, 137, 1268
BspTNI	GGTCTC	2 cut(s)	428, 566
BsrDI	GCAATG	3 cut(s)	489, 1095, 1297
BsrFI	RCCGGY	1 cut(s)	134
BsrI	ACTGG	7 cut(s)	199, 244, 859, 1270, 1348, 1402, 1509
BssAI	RCCGGY	1 cut(s)	134
BssECI	CCNNGG	7 cut(s)	516, 772, 787, 1328, 1472, 1491, 1705
BssMI	GATC	8 cut(s)	390, 525, 546, 752, 957, 1160, 1564, 1613
BssNI	GRCGYC	2 cut(s)	126, 237
BssSI	CACGAG	1 cut(s)	256
BssT1I	CCWWGG	2 cut(s)	1472, 1705
Bst2BI	CACGAG	1 cut(s)	256
Bst2UI	CCWGG	5 cut(s)	581, 668, 913, 1211, 1611
Bst4CI	ACNGT	9 cut(s)	56, 91, 142, 439, 838, 1256, 1316, 1495, 1690
Bst6I	CTCTTC	3 cut(s)	172, 280, 1081
BstACI	GRCGYC	2 cut(s)	126, 237
BstAPI	GCANNNNNTGC	1 cut(s)	1339
BstBAI	YACGTR	1 cut(s)	832
BstC8I	GCNNGC	5 cut(s)	48, 71, 77, 372, 658
BstDEI	CTNAG	2 cut(s)	1368, 1725
BstDSI	CCRYGG	1 cut(s)	1491
BstF5I	GGATG	7 cut(s)	144, 493, 634, 853, 1048, 1701, 1748
BstFNI	CGCG	1 cut(s)	827
BstH2I	RGCGCY	4 cut(s)	129, 314, 420, 555
BstHHI	GCGC	7 cut(s)	46, 75, 128, 313, 419, 554, 829
BstKTI	GATC	8 cut(s)	393, 528, 549, 755, 960, 1163, 1567, 1616
BstMAI	GTCTC	5 cut(s)	62, 97, 428, 566, 786
BstMBI	GATC	8 cut(s)	390, 525, 546, 752, 957, 1160, 1564, 1613
BstMWI	GCNNNNNNNGC	9 cut(s)	43, 319, 328, 677, 783, 887, 1073, 1133, 1339
BstNI	CCWGG	5 cut(s)	581, 668, 913, 1211, 1611
BstNSI	RCATGY	2 cut(s)	79, 335
BstSFI	CTRYAG	3 cut(s)	806, 1077, 1186
BstSLI	GKGCMC	2 cut(s)	897, 1421
BstUI	CGCG	1 cut(s)	827
BstV1I	GCAGC	5 cut(s)	789, 792, 1120, 1307, 1688
BstV2I	GAAGAC	2 cut(s)	545, 600
BstX2I	RGATCY	1 cut(s)	752
BstXI	CCANNNNNNTGG	2 cut(s)	1539, 1604
BstYI	RGATCY	1 cut(s)	752
BsuI	GTATCC	1 cut(s)	731
BsuRI	GGCC	4 cut(s)	113, 350, 671, 1654
BtgI	CCRYGG	1 cut(s)	1491
BtgZI	GCGATG	1 cut(s)	647
BtrI	CACGTC	1 cut(s)	543
BtsCI	GGATG	7 cut(s)	144, 493, 634, 853, 1048, 1701, 1748
BtsI	GCAGTG	2 cut(s)	1122, 1362
BtsIMutI	CAGTG	4 cut(s)	1122, 1263, 1362, 1395
BveI	ACCTGC	1 cut(s)	584
Cac8I	GCNNGC	5 cut(s)	48, 71, 77, 372, 658
CaiI	CAGNNNCTG	1 cut(s)	1670
CfoI	GCGC	7 cut(s)	46, 75, 128, 313, 419, 554, 829
Cfr10I	RCCGGY	1 cut(s)	134
Cfr13I	GGNCC	3 cut(s)	1381, 1607, 1653
Cfr9I	CCCGGG	2 cut(s)	772, 787
CseI	GACGC	1 cut(s)	226
CsiI	ACCWGGT	1 cut(s)	911
Csp6I	GTAC	4 cut(s)	247, 761, 943, 1269
CviAII	CATG	8 cut(s)	76, 332, 559, 961, 1282, 1444, 1559, 1638
CviQI	GTAC	4 cut(s)	247, 761, 943, 1269
DdeI	CTNAG	2 cut(s)	1368, 1725
DinI	GGCGCC	1 cut(s)	127
DpnI	GATC	8 cut(s)	392, 527, 548, 754, 959, 1162, 1566, 1615
DpnII	GATC	8 cut(s)	390, 525, 546, 752, 957, 1160, 1564, 1613
EaeI	YGGCCR	2 cut(s)	111, 669
Eam1104I	CTCTTC	3 cut(s)	172, 280, 1081
EarI	CTCTTC	3 cut(s)	172, 280, 1081
Ecl136II	GAGCTC	1 cut(s)	37
Eco130I	CCWWGG	2 cut(s)	1472, 1705
Eco147I	AGGCCT	1 cut(s)	350
Eco24I	GRGCYC	1 cut(s)	39
Eco31I	GGTCTC	2 cut(s)	428, 566
Eco47I	GGWCC	2 cut(s)	1381, 1607
Eco47III	AGCGCT	2 cut(s)	312, 418
Eco53kI	GAGCTC	1 cut(s)	37
Eco57I	CTGAAG	2 cut(s)	557, 1692
Eco72I	CACGTG	1 cut(s)	832
Eco88I	CYCGRG	4 cut(s)	265, 764, 772, 787
EcoICRI	GAGCTC	1 cut(s)	37
EcoO109I	RGGNCCY	1 cut(s)	1653
EcoRI	GAATTC	1 cut(s)	620
EcoRII	CCWGG	5 cut(s)	579, 666, 911, 1209, 1609
EcoT14I	CCWWGG	2 cut(s)	1472, 1705
EcoT22I	ATGCAT	1 cut(s)	1207
EcoT38I	GRGCYC	1 cut(s)	39
EgeI	GGCGCC	1 cut(s)	127
EheI	GGCGCC	1 cut(s)	127
ErhI	CCWWGG	2 cut(s)	1472, 1705
Esp3I	CGTCTC	2 cut(s)	62, 97
FaeI	CATG	8 cut(s)	79, 335, 562, 964, 1285, 1447, 1562, 1641
FalI	AAGNNNNNCTT	2 cut(s)	890, 922
FaqI	GGGAC	3 cut(s)	310, 865, 952
FatI	CATG	8 cut(s)	75, 331, 558, 960, 1281, 1443, 1558, 1637
FauI	CCCGC	2 cut(s)	74, 199
FbaI	TGATCA	1 cut(s)	957
FblI	GTMKAC	2 cut(s)	30, 1468
Fnu4HI	GCNGC	6 cut(s)	235, 803, 806, 1134, 1296, 1677
FokI	GGATG	7 cut(s)	131, 500, 641, 860, 1055, 1688, 1735
FriOI	GRGCYC	1 cut(s)	39
Fsp4HI	GCNGC	6 cut(s)	235, 803, 806, 1134, 1296, 1677
GlaI	GCGC	7 cut(s)	45, 74, 127, 312, 418, 553, 828
GluI	GCNGC	6 cut(s)	235, 803, 806, 1134, 1296, 1677
GsaI	CCCAGC	1 cut(s)	781
GsuI	CTGGAG	1 cut(s)	1149
HaeII	RGCGCY	4 cut(s)	129, 314, 420, 555
HaeIII	GGCC	4 cut(s)	113, 350, 671, 1654
HapII	CCGG	7 cut(s)	135, 305, 498, 716, 724, 773, 788
HgaI	GACGC	1 cut(s)	226
HhaI	GCGC	7 cut(s)	46, 75, 128, 313, 419, 554, 829
Hin1I	GRCGYC	2 cut(s)	126, 237
Hin1II	CATG	8 cut(s)	79, 335, 562, 964, 1285, 1447, 1562, 1641
Hin6I	GCGC	7 cut(s)	44, 73, 126, 311, 417, 552, 827
HinP1I	GCGC	7 cut(s)	44, 73, 126, 311, 417, 552, 827
HincII	GTYRAC	2 cut(s)	31, 204
HindII	GTYRAC	2 cut(s)	31, 204
HindIII	AAGCTT	1 cut(s)	461
HinfI	GANTC	8 cut(s)	205, 470, 584, 1234, 1517, 1552, 1694, 1745
HpaII	CCGG	7 cut(s)	135, 305, 498, 716, 724, 773, 788
HphI	GGTGA	3 cut(s)	79, 232, 713
Hpy166II	GTNNAC	8 cut(s)	31, 204, 721, 1419, 1469, 1542, 1607, 1686
Hpy188I	TCNGA	7 cut(s)	395, 517, 737, 1160, 1171, 1413, 1728
Hpy188III	TCNNGA	7 cut(s)	265, 590, 617, 875, 1250, 1309, 1525
Hpy8I	GTNNAC	8 cut(s)	31, 204, 721, 1419, 1469, 1542, 1607, 1686
Hpy99I	CGWCG	2 cut(s)	35, 178
HpyAV	CCTTC	3 cut(s)	19, 838, 1708
HpyCH4III	ACNGT	9 cut(s)	56, 91, 142, 439, 838, 1256, 1316, 1495, 1690
HpyCH4IV	ACGT	3 cut(s)	542, 831, 941
HpyF10VI	GCNNNNNNNGC	9 cut(s)	43, 319, 328, 677, 783, 887, 1073, 1133, 1339
HpyF3I	CTNAG	2 cut(s)	1368, 1725
HpySE526I	ACGT	3 cut(s)	542, 831, 941
Hsp92I	GRCGYC	2 cut(s)	126, 237
Hsp92II	CATG	8 cut(s)	79, 335, 562, 964, 1285, 1447, 1562, 1641
HspAI	GCGC	7 cut(s)	44, 73, 126, 311, 417, 552, 827
KasI	GGCGCC	1 cut(s)	125
KpnI	GGTACC	1 cut(s)	1272
Ksp22I	TGATCA	1 cut(s)	957
Kzo9I	GATC	8 cut(s)	390, 525, 546, 752, 957, 1160, 1564, 1613
LmnI	GCTCC	3 cut(s)	167, 1130, 1456
Lsp1109I	GCAGC	5 cut(s)	789, 792, 1120, 1307, 1688
LweI	GCATC	6 cut(s)	279, 439, 619, 624, 1181, 1192
MabI	ACCWGGT	1 cut(s)	911
MaeII	ACGT	3 cut(s)	542, 831, 941
MaeIII	GTNAC	8 cut(s)	85, 130, 238, 832, 1250, 1256, 1316, 1646
MalI	GATC	8 cut(s)	392, 527, 548, 754, 959, 1162, 1566, 1615
MboI	GATC	8 cut(s)	390, 525, 546, 752, 957, 1160, 1564, 1613
MflI	RGATCY	1 cut(s)	752
MhlI	GDGCHC	3 cut(s)	39, 897, 1421
MlsI	TGGCCA	1 cut(s)	671
MluCI	AATT	8 cut(s)	286, 601, 620, 684, 703, 1241, 1407, 1738
MluNI	TGGCCA	1 cut(s)	671
Mly113I	GGCGCC	1 cut(s)	126
MlyI	GAGTC	5 cut(s)	199, 578, 1546, 1703, 1754
MmeI	TCCRAC	3 cut(s)	315, 1194, 1478
Mox20I	TGGCCA	1 cut(s)	671
Mph1103I	ATGCAT	1 cut(s)	1207
MscI	TGGCCA	1 cut(s)	671
MseI	TTAA	6 cut(s)	221, 342, 423, 465, 864, 1119
MslI	CAYNNNNRTG	2 cut(s)	1537, 1753
Msp20I	TGGCCA	1 cut(s)	671
MspA1I	CMGCKG	1 cut(s)	1298
MspI	CCGG	7 cut(s)	135, 305, 498, 716, 724, 773, 788
Mva1269I	GAATGC	3 cut(s)	892, 1154, 1205
MvaI	CCWGG	5 cut(s)	581, 668, 913, 1211, 1611
MvnI	CGCG	1 cut(s)	827
MwoI	GCNNNNNNNGC	9 cut(s)	43, 319, 328, 677, 783, 887, 1073, 1133, 1339
NarI	GGCGCC	1 cut(s)	126
NciI	CCSGG	5 cut(s)	305, 773, 774, 788, 789
NdeII	GATC	8 cut(s)	390, 525, 546, 752, 957, 1160, 1564, 1613
NlaIII	CATG	8 cut(s)	79, 335, 562, 964, 1285, 1447, 1562, 1641
NlaIV	GGNNCC	7 cut(s)	127, 139, 189, 1168, 1270, 1383, 1452
NmeAIII	GCCGAG	1 cut(s)	1353
NmuCI	GTSAC	5 cut(s)	85, 238, 832, 1250, 1256
NsiI	ATGCAT	1 cut(s)	1207
NspI	RCATGY	2 cut(s)	79, 335
PaeI	GCATGC	1 cut(s)	79
PaeR7I	CTCGAG	1 cut(s)	265
PceI	AGGCCT	1 cut(s)	350
PcsI	WCGNNNNNNNCGW	5 cut(s)	27, 36, 176, 263, 342
PctI	GAATGC	3 cut(s)	892, 1154, 1205
PfeI	GAWTC	3 cut(s)	470, 1234, 1517
Pfl23II	CGTACG	1 cut(s)	942
PfoI	TCCNGGA	1 cut(s)	1209
PkrI	GCNGC	6 cut(s)	236, 804, 807, 1135, 1297, 1678
PleI	GAGTC	5 cut(s)	199, 578, 1546, 1702, 1753
PluTI	GGCGCC	1 cut(s)	129
PmaCI	CACGTG	1 cut(s)	832
PmlI	CACGTG	1 cut(s)	832
PpsI	GAGTC	5 cut(s)	199, 578, 1546, 1702, 1753
Ppu21I	YACGTR	1 cut(s)	832
Psp124BI	GAGCTC	1 cut(s)	39
Psp6I	CCWGG	5 cut(s)	579, 666, 911, 1209, 1609
PspCI	CACGTG	1 cut(s)	832
PspFI	CCCAGC	1 cut(s)	777
PspGI	CCWGG	5 cut(s)	579, 666, 911, 1209, 1609
PspLI	CGTACG	1 cut(s)	942
PspN4I	GGNNCC	7 cut(s)	127, 139, 189, 1168, 1270, 1383, 1452
PspPI	GGNCC	3 cut(s)	1381, 1607, 1653
PstI	CTGCAG	1 cut(s)	810
PstNI	CAGNNNCTG	1 cut(s)	1670
PsuI	RGATCY	1 cut(s)	752
PvuII	CAGCTG	1 cut(s)	1298
RsaI	GTAC	4 cut(s)	248, 762, 944, 1270
RsaNI	GTAC	4 cut(s)	247, 761, 943, 1269
RseI	CAYNNNNRTG	2 cut(s)	1537, 1753
SacI	GAGCTC	1 cut(s)	39
SalI	GTCGAC	1 cut(s)	29
SaqAI	TTAA	6 cut(s)	221, 342, 423, 465, 864, 1119
SatI	GCNGC	6 cut(s)	235, 803, 806, 1134, 1296, 1677
Sau3AI	GATC	8 cut(s)	390, 525, 546, 752, 957, 1160, 1564, 1613
Sau96I	GGNCC	3 cut(s)	1381, 1607, 1653
SchI	GAGTC	5 cut(s)	199, 578, 1546, 1703, 1754
SduI	GDGCHC	3 cut(s)	39, 897, 1421
SexAI	ACCWGGT	1 cut(s)	911
SfaNI	GCATC	6 cut(s)	279, 439, 619, 624, 1181, 1192
SfcI	CTRYAG	3 cut(s)	806, 1077, 1186
SfoI	GGCGCC	1 cut(s)	127
Sfr274I	CTCGAG	1 cut(s)	265
SinI	GGWCC	2 cut(s)	1381, 1607
SlaI	CTCGAG	1 cut(s)	265
SmaI	CCCGGG	2 cut(s)	774, 789
SmiMI	CAYNNNNRTG	2 cut(s)	1537, 1753
SmlI	CTYRAG	1 cut(s)	265
SmoI	CTYRAG	1 cut(s)	265
SphI	GCATGC	1 cut(s)	79
Sse9I	AATT	8 cut(s)	286, 601, 620, 684, 703, 1241, 1407, 1738
SseBI	AGGCCT	1 cut(s)	350
SsiI	CCGC	7 cut(s)	67, 154, 192, 213, 234, 825, 1148
SspDI	GGCGCC	1 cut(s)	125
SstI	GAGCTC	1 cut(s)	39
StuI	AGGCCT	1 cut(s)	350
StyI	CCWWGG	2 cut(s)	1472, 1705
TaaI	ACNGT	9 cut(s)	56, 91, 142, 439, 838, 1256, 1316, 1495, 1690
TaiI	ACGT	3 cut(s)	545, 834, 944
TasI	AATT	8 cut(s)	286, 601, 620, 684, 703, 1241, 1407, 1738
TauI	GCSGC	1 cut(s)	237
TfiI	GAWTC	3 cut(s)	470, 1234, 1517
Tru1I	TTAA	6 cut(s)	221, 342, 423, 465, 864, 1119
Tru9I	TTAA	6 cut(s)	221, 342, 423, 465, 864, 1119
TscAI	CASTG	4 cut(s)	1129, 1270, 1369, 1402
TseFI	GTSAC	5 cut(s)	85, 238, 832, 1250, 1256
TseI	GCWGC	5 cut(s)	802, 805, 1133, 1295, 1676
Tsp45I	GTSAC	5 cut(s)	85, 238, 832, 1250, 1256
TspDTI	ATGAA	5 cut(s)	1084, 1105, 1270, 1690, 1728
TspGWI	ACGGA	5 cut(s)	85, 200, 748, 756, 1394
TspMI	CCCGGG	2 cut(s)	772, 787
TspRI	CASTG	4 cut(s)	1129, 1270, 1369, 1402
VneI	GTGCAC	1 cut(s)	1417
VpaK11BI	GGWCC	2 cut(s)	1381, 1607
XapI	RAATTY	4 cut(s)	286, 620, 684, 1738
XceI	RCATGY	2 cut(s)	79, 335
XcmI	CCANNNNNNNNNTGG	1 cut(s)	664
XhoI	CTCGAG	1 cut(s)	265
XmaI	CCCGGG	2 cut(s)	772, 787
XmiI	GTMKAC	2 cut(s)	30, 1468
Zsp2I	ATGCAT	1 cut(s)	1207

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.