Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0015780

Physical Location & Seq

Genomic Coordinates Reverse (-)

63240324 .. 63243887

Gene Structure 3564 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020286

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1767 bp

ATGGACTCCAGGACCTCCTTTAAGGGCTACGGCAAAGTCGACGAGCTCGAAGAGCAAGCCTTCCGTCTCAAAACCCGCAAGCGCTTGCTCATCGTCACCGTCTCCGTCATTGTCCTCTTGGCCGTCATCACCGGCGCCGTCACCGGCACCGTCATCCACAACCGCAACAGCTCCTCTTCGCCGTCGCCGAACACGGAGCCCGCCACCCAGTTGACTCCCTCCGCTTCCTTAAAAGCCGTCTGCGGCGTCACCCAGTACCCCAACTCGTGCTTCTCCAGCATCTCTTCTCTCAAAAACTCCAACACCACCGACCCGGAAGCGCTGTTCAAGCTGTCCCTGCATGTCGCCATTAACGAGGCCTCGAAGCTCAAAGACTTGCCCACAGCAATAAAGAGTAAGATCGGAAATGATACCAAACTCGGAAGCGCTATTAAGGTCTGCGAGACCGTTTTCGATGATGCTGTGGATAAGCTTAACGATTCGCTCTCTGCAATGGATGTCAGTTCCGGCGAGAAGCTGTTGTCCGAGGCGAAGATCAATGACCTGAAGACGTGGATCAGCGCCACCATGACTGACCAAGGGACCTGCCTGGACTCTCTTGAAGACATCAATTCGACTTATGTTCAGGAATTCAGGGATGCTATGCAGAACTCCACAGAATACGCCAGCAACAGCCTGGCCATTGTGGCGAAAATTCTGGGGTTATTGTCCAATTTGAACATTCCGGTGCACCGGAGATTGCTCGGGTACGGAGGAGAAAGATCCTCTGTACCCGAGTTCCCGGGCTGGGTCAGCCCGGGAGACAGGAGGCTGCTGCAGGAGCAGAACCTGACTGCGCACGCGACCGTGGCGCAGGATGGGACTGGGGATTTTAAGACCATTCAGGAGGCTGTGAATGCTGTGCCCAAGAAGAACTTGACCAGGTTTGTGATATATGTGAAGCAAGGGACGTACAAAGAAAATGTGATCATGGACAAGAGCAAATGGAATGTGATGATGTATGGAGATAGCAAGACCAAGACCATTGTTTCTGGGAACTTGAACTATGTGGATGGGACTGCAACTTTCAACACAGCCACTTTTGCTGTTGCAGGAAGAGGGTTCATTGCGAAAGATATAGGGTTCATTAACACTGCTGGAGCTGTAAAGCACCAAGCGGTGGCATTCCGATCTGGTTCCGACCAATCTGTGTACTACAGATGCTCATTTGATGCATTCCAGGACACTCTCTATGCTCACTCAAATCGACAATTCTATCGTGATTGTGACATCACTGGCACCATTGACTTCATCTTTGGCAATGCAGCTGTGGTGTTCCAAAACTGTAACATCTGGCCGAGGCAACCAATGCTAAACCAGTTCAACACAATCACTGCTCAGGGAAAGACGGATCCCAATCAAAACACTGGGATTTCAATCCAAAAGTGCACAATCTCTCCACTTGAAAGCAACATGACAGCTCTAACATATCTTGGTAGACCTTGGAAGGAGTATTCCACCACGGTTTTCATGCAGTCCACTATTGATTCATTCTTGAACCCAGCGGGGTGGAAAGAATGGGTTTCCAATGTTGACCCACCTAACACCATATTTTATGCAGAGTACCAAAATAGAGGACCAGGATCGAGTGTGGATAAAAGGGTTACATGGGCTGGTTACAGGCCCTCTCTCACAGATACTGAAGCAGCCAAGTTCACAGTTGAGTCCTTCATCCAAGGTAGTGAATGGTTGCCTCAGACTAATGTGGAGTTTGAGACATCCCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0008150 GO:0009506 GO:0012505 GO:0016020 GO:0016787 GO:0016788 GO:0030054 GO:0030234 GO:0030599 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0043086 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044422 GO:0044424 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046910 GO:0050790 GO:0051722 GO:0051723 GO:0052689 GO:0055044 GO:0065007 GO:0065009 GO:0071944 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098805 GO:0140096

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

589

Amino Acids

64.7

Weight (kDa)

6.37

Isoelectric Point (pI)

28.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	76 - 228	2.1e-30	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	281 - 573	9.1e-139	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015780)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53840 AT3G14300
fragaria_vesca	FvH4_6g35820
malus_domestica	MD09G1172900.v1.1 MD17G1154400.v1.1
rosa_chinensis	RchiOBHm_Chr2g0147861
rosa_laevigata	RLG00000020286
rosa_multiflora	Rmu_sc0000308.1_g000054
rosa_roxburghii	Rroxscaffold_2G00099260
rosa_rugosa	Rorug02G0402500
rosa_samantha	Rh2AG459400 Rh2BG472800 Rh2CG446800 Rh2DG481700
rosa_wichuraiana	Rw2G037580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	837
Acc36I	ACCTGC	1 cut(s)	593
AccB1I	GGYRCC	3 cut(s)	134, 146, 1277
AccB7I	CCANNNNNTGG	1 cut(s)	1159
AccI	GTMKAC	2 cut(s)	39, 1477
AccII	CGCG	1 cut(s)	842
AciI	CCGC	7 cut(s)	76, 163, 201, 222, 243, 1157, 1544
AclWI	GGATC	5 cut(s)	563, 756, 1385, 1398, 1630
AcoI	YGGCCR	3 cut(s)	120, 678, 1334
AcsI	RAATTY	2 cut(s)	629, 693
AcuI	CTGAAG	2 cut(s)	566, 1701
AcyI	GRCGYC	2 cut(s)	135, 246
AfaI	GTAC	6 cut(s)	257, 749, 771, 953, 1193, 1604
AfeI	AGCGCT	3 cut(s)	83, 321, 427
AfiI	CCNNNNNNNGG	2 cut(s)	787, 1159
AgsI	TTSAA	9 cut(s)	328, 602, 718, 1042, 1069, 1363, 1416, 1445, 1537
AjiI	CACGTC	1 cut(s)	552
AjnI	CCWGG	6 cut(s)	8, 588, 675, 920, 1218, 1618
AjuI	GAANNNNNNNTTGG	2 cut(s)	254, 286
AloI	GAACNNNNNNTCC	2 cut(s)	308, 340
AluBI	AGCT	9 cut(s)	46, 171, 331, 367, 472, 517, 1142, 1307, 1460
AluI	AGCT	9 cut(s)	46, 171, 331, 367, 472, 517, 1142, 1307, 1460
Alw21I	GWGCWC	3 cut(s)	48, 732, 1430
Alw26I	GTCTC	5 cut(s)	71, 106, 437, 795, 1748
Alw44I	GTGCAC	2 cut(s)	728, 1426
AlwI	GGATC	5 cut(s)	563, 756, 1385, 1398, 1630
AlwNI	CAGNNNCTG	2 cut(s)	829, 1679
Ama87I	CYCGRG	4 cut(s)	743, 773, 781, 796
Aor51HI	AGCGCT	3 cut(s)	83, 321, 427
AoxI	GGCC	5 cut(s)	120, 357, 678, 1334, 1661
ApaLI	GTGCAC	2 cut(s)	728, 1426
ApeKI	GCWGC	4 cut(s)	811, 814, 1304, 1685
ApoI	RAATTY	2 cut(s)	629, 693
ArsI	GACNNNNNNTTYG	2 cut(s)	1277, 1309
AspLEI	GCGC	7 cut(s)	84, 137, 322, 428, 563, 838, 853
AspS9I	GGNCC	4 cut(s)	12, 582, 1616, 1662
AsuC2I	CCSGG	5 cut(s)	314, 782, 783, 797, 798
AsuHPI	GGTGA	4 cut(s)	88, 121, 133, 241
AvaI	CYCGRG	4 cut(s)	743, 773, 781, 796
AvaII	GGWCC	3 cut(s)	12, 582, 1616
BaeGI	GKGCMC	3 cut(s)	732, 906, 1430
BalI	TGGCCA	1 cut(s)	680
BamHI	GGATCC	1 cut(s)	1390
BanI	GGYRCC	3 cut(s)	134, 146, 1277
BanII	GRGCYC	2 cut(s)	48, 201
BauI	CACGAG	1 cut(s)	265
BbsI	GAAGAC	2 cut(s)	554, 609
Bbv12I	GWGCWC	3 cut(s)	48, 732, 1430
BbvI	GCAGC	4 cut(s)	798, 801, 1316, 1697
BccI	CCATC	2 cut(s)	851, 1046
BceAI	ACGGC	5 cut(s)	46, 107, 122, 166, 221
BciT130I	CCWGG	6 cut(s)	10, 590, 677, 922, 1220, 1620
BclI	TGATCA	1 cut(s)	966
BcnI	CCSGG	5 cut(s)	314, 782, 783, 797, 798
BcoDI	GTCTC	5 cut(s)	71, 106, 437, 795, 1748
BfmI	CTRYAG	2 cut(s)	815, 1195
BfoI	RGCGCY	5 cut(s)	85, 138, 323, 429, 564
BfuAI	ACCTGC	1 cut(s)	593
BglI	GCCNNNNNGGC	1 cut(s)	686
BisI	GCNGC	5 cut(s)	244, 812, 815, 1305, 1686
BlsI	GCNGC	5 cut(s)	245, 813, 816, 1306, 1687
Bme18I	GGWCC	3 cut(s)	12, 582, 1616
BmeT110I	CYCGRG	4 cut(s)	743, 773, 781, 796
BmgBI	CACGTC	1 cut(s)	552
BmgT120I	GGNCC	4 cut(s)	12, 582, 1616, 1662
BmiI	GGNNCC	7 cut(s)	136, 148, 198, 583, 1177, 1279, 1392
BmrI	ACTGGG	4 cut(s)	202, 247, 873, 1416
BmsI	GCATC	5 cut(s)	288, 448, 628, 1190, 1201
BmuI	ACTGGG	4 cut(s)	202, 247, 873, 1416
BpiI	GAAGAC	2 cut(s)	554, 609
BpmI	CTGGAG	2 cut(s)	259, 1158
Bpu10I	CCTNAGC	1 cut(s)	1377
BpuMI	CCSGG	5 cut(s)	314, 782, 783, 797, 798
BsaBI	GATNNNNATC	2 cut(s)	1395, 1415
BsaHI	GRCGYC	2 cut(s)	135, 246
BsaI	GGTCTC	1 cut(s)	437
BsaJI	CCNNGG	9 cut(s)	525, 577, 781, 796, 846, 1337, 1481, 1500, 1714
BsaWI	WCCGGW	2 cut(s)	724, 732
BsaXI	ACNNNNNCTCC	2 cut(s)	1420, 1450
Bsc4I	CCNNNNNNNGG	2 cut(s)	787, 1159
Bse118I	RCCGGY	2 cut(s)	131, 143
Bse1I	ACTGG	6 cut(s)	208, 253, 868, 1279, 1357, 1411
Bse3DI	GCAATG	3 cut(s)	498, 1104, 1306
Bse8I	GATNNNNATC	2 cut(s)	1395, 1415
BseBI	CCWGG	6 cut(s)	10, 590, 677, 922, 1220, 1620
BseDI	CCNNGG	9 cut(s)	525, 577, 781, 796, 846, 1337, 1481, 1500, 1714
BseGI	GGATG	7 cut(s)	153, 502, 643, 862, 1057, 1710, 1757
BseJI	GATNNNNATC	2 cut(s)	1395, 1415
BseLI	CCNNNNNNNGG	2 cut(s)	787, 1159
BseMI	GCAATG	3 cut(s)	498, 1104, 1306
BseMII	CTCAG	2 cut(s)	1391, 1748
BseNI	ACTGG	6 cut(s)	208, 253, 868, 1279, 1357, 1411
BseRI	GAGGAG	2 cut(s)	163, 768
BseSI	GKGCMC	3 cut(s)	732, 906, 1430
BseXI	GCAGC	4 cut(s)	798, 801, 1316, 1697
BseYI	CCCAGC	2 cut(s)	786, 1540
Bsh1236I	CGCG	1 cut(s)	842
Bsh1285I	CGRYCG	1 cut(s)	846
BshFI	GGCC	5 cut(s)	122, 359, 680, 1336, 1663
BshNI	GGYRCC	3 cut(s)	134, 146, 1277
BsiEI	CGRYCG	1 cut(s)	846
BsiHKAI	GWGCWC	3 cut(s)	48, 732, 1430
BsiHKCI	CYCGRG	4 cut(s)	743, 773, 781, 796
BsiSI	CCGG	8 cut(s)	132, 144, 314, 507, 725, 733, 782, 797
BslFI	GGGAC	5 cut(s)	319, 595, 874, 961, 1069
BslI	CCNNNNNNNGG	2 cut(s)	787, 1159
BsmAI	GTCTC	5 cut(s)	71, 106, 437, 795, 1748
BsmBI	CGTCTC	2 cut(s)	71, 106
BsmFI	GGGAC	5 cut(s)	319, 595, 874, 961, 1069
BsmI	GAATGC	3 cut(s)	901, 1163, 1214
BsnI	GGCC	5 cut(s)	122, 359, 680, 1336, 1663
Bso31I	GGTCTC	1 cut(s)	437
BsoBI	CYCGRG	4 cut(s)	743, 773, 781, 796
Bsp1286I	GDGCHC	5 cut(s)	48, 201, 732, 906, 1430
Bsp143I	GATC	8 cut(s)	399, 534, 555, 761, 966, 1169, 1390, 1622
BspACI	CCGC	7 cut(s)	76, 163, 201, 222, 243, 1157, 1544
BspANI	GGCC	5 cut(s)	122, 359, 680, 1336, 1663
BspCNI	CTCAG	2 cut(s)	1390, 1747
BspFNI	CGCG	1 cut(s)	842
BspLI	GGNNCC	7 cut(s)	136, 148, 198, 583, 1177, 1279, 1392
BspMAI	CTGCAG	1 cut(s)	819
BspMI	ACCTGC	1 cut(s)	593
BspPI	GGATC	5 cut(s)	563, 756, 1385, 1398, 1630
BspQI	GCTCTTC	1 cut(s)	45
BspT107I	GGYRCC	3 cut(s)	134, 146, 1277
BspTNI	GGTCTC	1 cut(s)	437
BsrDI	GCAATG	3 cut(s)	498, 1104, 1306
BsrFI	RCCGGY	2 cut(s)	131, 143
BsrI	ACTGG	6 cut(s)	208, 253, 868, 1279, 1357, 1411
BssAI	RCCGGY	2 cut(s)	131, 143
BssECI	CCNNGG	9 cut(s)	525, 577, 781, 796, 846, 1337, 1481, 1500, 1714
BssMI	GATC	8 cut(s)	399, 534, 555, 761, 966, 1169, 1390, 1622
BssNI	GRCGYC	2 cut(s)	135, 246
BssSI	CACGAG	1 cut(s)	265
BssT1I	CCWWGG	3 cut(s)	577, 1481, 1714
Bst2BI	CACGAG	1 cut(s)	265
Bst2UI	CCWGG	6 cut(s)	10, 590, 677, 922, 1220, 1620
Bst4CI	ACNGT	7 cut(s)	100, 151, 448, 847, 1325, 1504, 1699
Bst6I	CTCTTC	4 cut(s)	45, 181, 289, 1090
BstACI	GRCGYC	2 cut(s)	135, 246
BstAPI	GCANNNNNTGC	1 cut(s)	1348
BstC8I	GCNNGC	6 cut(s)	57, 80, 86, 201, 667, 840
BstDEI	CTNAG	2 cut(s)	1377, 1734
BstDSI	CCRYGG	2 cut(s)	846, 1500
BstF5I	GGATG	7 cut(s)	153, 502, 643, 862, 1057, 1710, 1757
BstFNI	CGCG	1 cut(s)	842
BstH2I	RGCGCY	5 cut(s)	85, 138, 323, 429, 564
BstHHI	GCGC	7 cut(s)	84, 137, 322, 428, 563, 838, 853
BstKTI	GATC	8 cut(s)	402, 537, 558, 764, 969, 1172, 1393, 1625
BstMAI	GTCTC	5 cut(s)	71, 106, 437, 795, 1748
BstMBI	GATC	8 cut(s)	399, 534, 555, 761, 966, 1169, 1390, 1622
BstMCI	CGRYCG	1 cut(s)	846
BstNI	CCWGG	6 cut(s)	10, 590, 677, 922, 1220, 1620
BstNSI	RCATGY	1 cut(s)	344
BstSFI	CTRYAG	2 cut(s)	815, 1195
BstSLI	GKGCMC	3 cut(s)	732, 906, 1430
BstUI	CGCG	1 cut(s)	842
BstV1I	GCAGC	4 cut(s)	798, 801, 1316, 1697
BstV2I	GAAGAC	2 cut(s)	554, 609
BstX2I	RGATCY	2 cut(s)	761, 1390
BstXI	CCANNNNNNTGG	1 cut(s)	1548
BstYI	RGATCY	2 cut(s)	761, 1390
BsuRI	GGCC	5 cut(s)	122, 359, 680, 1336, 1663
BtgI	CCRYGG	2 cut(s)	846, 1500
BtrI	CACGTC	1 cut(s)	552
BtsCI	GGATG	7 cut(s)	153, 502, 643, 862, 1057, 1710, 1757
BtsI	GCAGTG	2 cut(s)	1131, 1371
BtsIMutI	CAGTG	4 cut(s)	1131, 1272, 1371, 1404
BveI	ACCTGC	1 cut(s)	593
Cac8I	GCNNGC	6 cut(s)	57, 80, 86, 201, 667, 840
CaiI	CAGNNNCTG	2 cut(s)	829, 1679
CfoI	GCGC	7 cut(s)	84, 137, 322, 428, 563, 838, 853
Cfr10I	RCCGGY	2 cut(s)	131, 143
Cfr13I	GGNCC	4 cut(s)	12, 582, 1616, 1662
Cfr9I	CCCGGG	2 cut(s)	781, 796
CseI	GACGC	1 cut(s)	235
CsiI	ACCWGGT	1 cut(s)	920
Csp6I	GTAC	6 cut(s)	256, 748, 770, 952, 1192, 1603
CviAII	CATG	6 cut(s)	341, 568, 970, 1453, 1510, 1647
CviQI	GTAC	6 cut(s)	256, 748, 770, 952, 1192, 1603
DdeI	CTNAG	2 cut(s)	1377, 1734
DinI	GGCGCC	1 cut(s)	136
DpnI	GATC	8 cut(s)	401, 536, 557, 763, 968, 1171, 1392, 1624
DpnII	GATC	8 cut(s)	399, 534, 555, 761, 966, 1169, 1390, 1622
EaeI	YGGCCR	3 cut(s)	120, 678, 1334
Eam1104I	CTCTTC	4 cut(s)	45, 181, 289, 1090
EarI	CTCTTC	4 cut(s)	45, 181, 289, 1090
Ecl136II	GAGCTC	1 cut(s)	46
Eco130I	CCWWGG	3 cut(s)	577, 1481, 1714
Eco147I	AGGCCT	1 cut(s)	359
Eco24I	GRGCYC	2 cut(s)	48, 201
Eco31I	GGTCTC	1 cut(s)	437
Eco47I	GGWCC	3 cut(s)	12, 582, 1616
Eco47III	AGCGCT	3 cut(s)	83, 321, 427
Eco53kI	GAGCTC	1 cut(s)	46
Eco57I	CTGAAG	2 cut(s)	566, 1701
Eco88I	CYCGRG	4 cut(s)	743, 773, 781, 796
EcoICRI	GAGCTC	1 cut(s)	46
EcoO109I	RGGNCCY	3 cut(s)	12, 582, 1662
EcoRI	GAATTC	1 cut(s)	629
EcoRII	CCWGG	6 cut(s)	8, 588, 675, 920, 1218, 1618
EcoT14I	CCWWGG	3 cut(s)	577, 1481, 1714
EcoT22I	ATGCAT	1 cut(s)	1216
EcoT38I	GRGCYC	2 cut(s)	48, 201
EgeI	GGCGCC	1 cut(s)	136
EheI	GGCGCC	1 cut(s)	136
ErhI	CCWWGG	3 cut(s)	577, 1481, 1714
Esp3I	CGTCTC	2 cut(s)	71, 106
FaeI	CATG	6 cut(s)	344, 571, 973, 1456, 1513, 1650
FalI	AAGNNNNNCTT	2 cut(s)	899, 931
FaqI	GGGAC	5 cut(s)	319, 595, 874, 961, 1069
FatI	CATG	6 cut(s)	340, 567, 969, 1452, 1509, 1646
FauI	CCCGC	3 cut(s)	83, 208, 1537
FbaI	TGATCA	1 cut(s)	966
FblI	GTMKAC	2 cut(s)	39, 1477
Fnu4HI	GCNGC	5 cut(s)	244, 812, 815, 1305, 1686
FokI	GGATG	7 cut(s)	140, 509, 650, 869, 1064, 1697, 1744
FriOI	GRGCYC	2 cut(s)	48, 201
Fsp4HI	GCNGC	5 cut(s)	244, 812, 815, 1305, 1686
FspI	TGCGCA	1 cut(s)	837
GlaI	GCGC	7 cut(s)	83, 136, 321, 427, 562, 837, 852
GluI	GCNGC	5 cut(s)	244, 812, 815, 1305, 1686
GsaI	CCCAGC	2 cut(s)	790, 1544
GsuI	CTGGAG	2 cut(s)	259, 1158
HaeII	RGCGCY	5 cut(s)	85, 138, 323, 429, 564
HaeIII	GGCC	5 cut(s)	122, 359, 680, 1336, 1663
HapII	CCGG	8 cut(s)	132, 144, 314, 507, 725, 733, 782, 797
HgaI	GACGC	1 cut(s)	235
HhaI	GCGC	7 cut(s)	84, 137, 322, 428, 563, 838, 853
Hin1I	GRCGYC	2 cut(s)	135, 246
Hin1II	CATG	6 cut(s)	344, 571, 973, 1456, 1513, 1650
Hin6I	GCGC	7 cut(s)	82, 135, 320, 426, 561, 836, 851
HinP1I	GCGC	7 cut(s)	82, 135, 320, 426, 561, 836, 851
HincII	GTYRAC	3 cut(s)	40, 213, 1573
HindII	GTYRAC	3 cut(s)	40, 213, 1573
HindIII	AAGCTT	1 cut(s)	470
HinfI	GANTC	6 cut(s)	5, 214, 479, 593, 1526, 1703
HpaII	CCGG	8 cut(s)	132, 144, 314, 507, 725, 733, 782, 797
HphI	GGTGA	4 cut(s)	88, 121, 133, 241
Hpy166II	GTNNAC	9 cut(s)	40, 213, 730, 1192, 1428, 1478, 1518, 1573, 1695
Hpy188I	TCNGA	6 cut(s)	404, 422, 526, 1169, 1180, 1737
Hpy188III	TCNNGA	5 cut(s)	599, 626, 884, 1259, 1534
Hpy8I	GTNNAC	9 cut(s)	40, 213, 730, 1192, 1428, 1478, 1518, 1573, 1695
Hpy99I	CGWCG	2 cut(s)	44, 187
HpyAV	CCTTC	3 cut(s)	70, 1480, 1717
HpyCH4III	ACNGT	7 cut(s)	100, 151, 448, 847, 1325, 1504, 1699
HpyCH4IV	ACGT	2 cut(s)	551, 950
HpyF3I	CTNAG	2 cut(s)	1377, 1734
HpySE526I	ACGT	2 cut(s)	551, 950
Hsp92I	GRCGYC	2 cut(s)	135, 246
Hsp92II	CATG	6 cut(s)	344, 571, 973, 1456, 1513, 1650
HspAI	GCGC	7 cut(s)	82, 135, 320, 426, 561, 836, 851
KasI	GGCGCC	1 cut(s)	134
Ksp22I	TGATCA	1 cut(s)	966
Kzo9I	GATC	8 cut(s)	399, 534, 555, 761, 966, 1169, 1390, 1622
LguI	GCTCTTC	1 cut(s)	45
LmnI	GCTCC	4 cut(s)	176, 196, 820, 1139
Lsp1109I	GCAGC	4 cut(s)	798, 801, 1316, 1697
LweI	GCATC	5 cut(s)	288, 448, 628, 1190, 1201
MabI	ACCWGGT	1 cut(s)	920
MaeII	ACGT	2 cut(s)	551, 950
MaeIII	GTNAC	7 cut(s)	94, 139, 247, 1265, 1325, 1642, 1655
MalI	GATC	8 cut(s)	401, 536, 557, 763, 968, 1171, 1392, 1624
MboI	GATC	8 cut(s)	399, 534, 555, 761, 966, 1169, 1390, 1622
MboII	GAAGA	8 cut(s)	62, 168, 276, 544, 559, 614, 922, 1107
MflI	RGATCY	2 cut(s)	761, 1390
MhlI	GDGCHC	5 cut(s)	48, 201, 732, 906, 1430
MlsI	TGGCCA	1 cut(s)	680
MluCI	AATT	5 cut(s)	610, 629, 693, 712, 1250
MluNI	TGGCCA	1 cut(s)	680
Mly113I	GGCGCC	1 cut(s)	135
MlyI	GAGTC	3 cut(s)	208, 587, 1712
MmeI	TCCRAC	2 cut(s)	324, 1203
Mox20I	TGGCCA	1 cut(s)	680
Mph1103I	ATGCAT	1 cut(s)	1216
MscI	TGGCCA	1 cut(s)	680
MseI	TTAA	7 cut(s)	21, 230, 351, 432, 474, 873, 1128
MslI	CAYNNNNRTG	2 cut(s)	725, 1762
Msp20I	TGGCCA	1 cut(s)	680
MspA1I	CMGCKG	2 cut(s)	1307, 1544
MspI	CCGG	8 cut(s)	132, 144, 314, 507, 725, 733, 782, 797
Mva1269I	GAATGC	3 cut(s)	901, 1163, 1214
MvaI	CCWGG	6 cut(s)	10, 590, 677, 922, 1220, 1620
MvnI	CGCG	1 cut(s)	842
NarI	GGCGCC	1 cut(s)	135
NciI	CCSGG	5 cut(s)	314, 782, 783, 797, 798
NdeII	GATC	8 cut(s)	399, 534, 555, 761, 966, 1169, 1390, 1622
NlaIII	CATG	6 cut(s)	344, 571, 973, 1456, 1513, 1650
NlaIV	GGNNCC	7 cut(s)	136, 148, 198, 583, 1177, 1279, 1392
NmeAIII	GCCGAG	1 cut(s)	1362
NmuCI	GTSAC	4 cut(s)	94, 139, 247, 1265
NsbI	TGCGCA	1 cut(s)	837
NsiI	ATGCAT	1 cut(s)	1216
NspI	RCATGY	1 cut(s)	344
PceI	AGGCCT	1 cut(s)	359
PciSI	GCTCTTC	1 cut(s)	45
PcsI	WCGNNNNNNNCGW	4 cut(s)	36, 45, 185, 351
PctI	GAATGC	3 cut(s)	901, 1163, 1214
PfeI	GAWTC	2 cut(s)	479, 1526
PflMI	CCANNNNNTGG	1 cut(s)	1159
PfoI	TCCNGGA	2 cut(s)	8, 1218
PkrI	GCNGC	5 cut(s)	245, 813, 816, 1306, 1687
PleI	GAGTC	3 cut(s)	208, 587, 1711
PluTI	GGCGCC	1 cut(s)	138
PpsI	GAGTC	3 cut(s)	208, 587, 1711
PpuMI	RGGWCCY	2 cut(s)	12, 582
Psp124BI	GAGCTC	1 cut(s)	48
Psp5II	RGGWCCY	2 cut(s)	12, 582
Psp6I	CCWGG	6 cut(s)	8, 588, 675, 920, 1218, 1618
PspFI	CCCAGC	2 cut(s)	786, 1540
PspGI	CCWGG	6 cut(s)	8, 588, 675, 920, 1218, 1618
PspN4I	GGNNCC	7 cut(s)	136, 148, 198, 583, 1177, 1279, 1392
PspPI	GGNCC	4 cut(s)	12, 582, 1616, 1662
PspPPI	RGGWCCY	2 cut(s)	12, 582
PstI	CTGCAG	1 cut(s)	819
PstNI	CAGNNNCTG	2 cut(s)	829, 1679
PsuI	RGATCY	2 cut(s)	761, 1390
PvuII	CAGCTG	1 cut(s)	1307
RsaI	GTAC	6 cut(s)	257, 749, 771, 953, 1193, 1604
RsaNI	GTAC	6 cut(s)	256, 748, 770, 952, 1192, 1603
RseI	CAYNNNNRTG	2 cut(s)	725, 1762
SacI	GAGCTC	1 cut(s)	48
SalI	GTCGAC	1 cut(s)	38
SapI	GCTCTTC	1 cut(s)	45
SaqAI	TTAA	7 cut(s)	21, 230, 351, 432, 474, 873, 1128
SatI	GCNGC	5 cut(s)	244, 812, 815, 1305, 1686
Sau3AI	GATC	8 cut(s)	399, 534, 555, 761, 966, 1169, 1390, 1622
Sau96I	GGNCC	4 cut(s)	12, 582, 1616, 1662
SchI	GAGTC	3 cut(s)	208, 587, 1712
SduI	GDGCHC	5 cut(s)	48, 201, 732, 906, 1430
SexAI	ACCWGGT	1 cut(s)	920
SfaNI	GCATC	5 cut(s)	288, 448, 628, 1190, 1201
SfcI	CTRYAG	2 cut(s)	815, 1195
SfoI	GGCGCC	1 cut(s)	136
SgrAI	CRCCGGYG	1 cut(s)	131
SinI	GGWCC	3 cut(s)	12, 582, 1616
SmaI	CCCGGG	2 cut(s)	783, 798
SmiMI	CAYNNNNRTG	2 cut(s)	725, 1762
Sse9I	AATT	5 cut(s)	610, 629, 693, 712, 1250
SseBI	AGGCCT	1 cut(s)	359
SsiI	CCGC	7 cut(s)	76, 163, 201, 222, 243, 1157, 1544
SspDI	GGCGCC	1 cut(s)	134
SstI	GAGCTC	1 cut(s)	48
StuI	AGGCCT	1 cut(s)	359
StyI	CCWWGG	3 cut(s)	577, 1481, 1714
TaaI	ACNGT	7 cut(s)	100, 151, 448, 847, 1325, 1504, 1699
TaiI	ACGT	2 cut(s)	554, 953
TaqI	TCGA	7 cut(s)	39, 48, 362, 453, 614, 1246, 1625
TasI	AATT	5 cut(s)	610, 629, 693, 712, 1250
TatI	WGTACW	1 cut(s)	1191
TauI	GCSGC	1 cut(s)	246
TfiI	GAWTC	2 cut(s)	479, 1526
Tru1I	TTAA	7 cut(s)	21, 230, 351, 432, 474, 873, 1128
Tru9I	TTAA	7 cut(s)	21, 230, 351, 432, 474, 873, 1128
TscAI	CASTG	4 cut(s)	1138, 1279, 1378, 1411
TseFI	GTSAC	4 cut(s)	94, 139, 247, 1265
TseI	GCWGC	4 cut(s)	811, 814, 1304, 1685
Tsp45I	GTSAC	4 cut(s)	94, 139, 247, 1265
TspDTI	ATGAA	6 cut(s)	1093, 1114, 1279, 1498, 1518, 1699
TspGWI	ACGGA	5 cut(s)	53, 94, 209, 765, 1403
TspMI	CCCGGG	2 cut(s)	781, 796
TspRI	CASTG	4 cut(s)	1138, 1279, 1378, 1411
Van91I	CCANNNNNTGG	1 cut(s)	1159
VneI	GTGCAC	2 cut(s)	728, 1426
VpaK11BI	GGWCC	3 cut(s)	12, 582, 1616
XapI	RAATTY	2 cut(s)	629, 693
XceI	RCATGY	1 cut(s)	344
XcmI	CCANNNNNNNNNTGG	1 cut(s)	673
XmaI	CCCGGG	2 cut(s)	781, 796
XmiI	GTMKAC	2 cut(s)	39, 1477
Zsp2I	ATGCAT	1 cut(s)	1216

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.