Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0015780

Physical Location & Seq

Genomic Coordinates Forward (+)

14644351 .. 14647956

Gene Structure 3606 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1172900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGGACTCCGTCAAGTCCTTCAAGGGCTACGGCAAAGTCGACGAGCTCGAAGAGCAGGCCTTCAAACGCAAGACCCGGAAGCGTCTCATCATCCTCTCAGTCTCCACGGTGGTTCTGTTGGCGGTGATCATCGGCGTTGTCGCGGGGGTGATCATACACAAGAGGAACAACTCGTCGTCTTCTGTACCCAACTCGACCCCCGGCCCCGAATTGACTCCGGCGGCGTCGCTGAAGGCGGTTTGCGGCGTGACCCAATACCCGAGTTCGTGTTTCTCGAGCATCTCCGCGCTCGAGACATCGAACAGCACCGACCCGGAAGTGATTTTCAAGCTCTCTCTGCAGGTCGCCATTCACGCCGCCGCGAAGCTTCCCGGGCTGCCGGCGAAAATCGGTCTGAACATCGCCAATGACACCCACCTGGAGAAAGTTCTAGACGTCTGCGAGGGCCTGTTTGAAGACGTGGTTGACAGGCTCAACGACTCGCTGACCTCCATGGATGTGAATCAGGGCGACAAGCTTCTCTCCGCCGCCAAAATCAACGACATCAAGACGTGGTTGAGCACCACATTGACGGATCAGGAGACTTGTTTGGACTCTCTGGCGGATCTCAACTCGACCCTCGTTCAAGAATTCAAGGCGGCGATGCAGAATACAACTGAATTTTCGAGCAATAGTTTGGCGATTGTGGCGAAAATCCTGAGCCTTCTTACGAATTTGAACGTTCCGGTTCACCGGAGGCTGCTCGGGGTCGGAGGATCAGAGGTGGATTTTCCGGGTTGGATTAGTCCGGGGGATAGGAGGCTGCTGCAGGACAACAATGCGACGGCGCAGATAGTGGTGGCGAAGGATGGGACGGGAGATTTTAAGACGATTCAGGAGGCGGTGGATACGGTGCCAAAGAAGAGCAAGAACAGGATTCTGATTCATGTGAAGCAAGGGGTTTATTATGAGAATGTGTTGATGGATAAGAGCAAGTGGAATGTGATGATGTTCGGAGATGGGAAGACGAAGACGGTGGTTTCCGGCAACTTGAGCTTTGTGGACGGCACCCCCACCTTCTCCACCGCCACTTTTGCCGTCACAGGAAAAGGCTTCATCGTGAAAGATATGGGATTCTTCAACACTGCAGGAGCAATAAAGCACCAGGCAGTGGCATTCCGCTCTGGTTCGGACCTCTCTGTGTACTACAGATGCTTGTTTGATGGCTTCCAGGACACCCTCTATGCGCATTCCAACCGACAATTCTATCGGGAATGTGACATTACCGGCACCATAGACTTCATTTTTGGCAATGCAGCTGTAGTTTTCCAGAACTGCAACATCCAGCCCAGGCAACCTATGGCGAACCAGTTCAACACGATCACAGCTCAAGGCAAAAAGGACCCGAATCAGAACACTGGCATTTCAATCCAAAAGTGCAGATTGTCTGCGTTAGACAGTAATCTCACTGCTCAAACATATCTGGGGAGGCCTTGGAAGGATTACTCCACCACGGTCATCATGCAGTCTGACATTAGATCATTCTTGAACCCATTGGGCTGGAAGGAATGGGTTACCAACGTTGACCCACCCAACACCATATTCTATGCAGAGTACCAAAACAGTGGGCCTGGATCAGGTGTGGATCAAAGGGTTAAGTGGGCCGGTTACAACCCTACTCTTACAGATGACGAGGCGGCCAAGTTCACGGTAACATCATTGATCCAAGGCGGTGAGTGGTTGCCGGCAACTGATGTGGCATTTGAGACTACCTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0008150 GO:0009506 GO:0012505 GO:0016020 GO:0016787 GO:0016788 GO:0030054 GO:0030234 GO:0030599 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0043086 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044422 GO:0044424 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046910 GO:0050790 GO:0051722 GO:0051723 GO:0052689 GO:0055044 GO:0065007 GO:0065009 GO:0071944 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098805 GO:0140096

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

586

Amino Acids

63.9

Weight (kDa)

6.22

Isoelectric Point (pI)

26.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	76 - 228	9.2e-28	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	278 - 570	2.8e-138	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015780)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53840 AT3G14300
fragaria_vesca	FvH4_6g35820
malus_domestica	MD09G1172900.v1.1 MD17G1154400.v1.1
rosa_chinensis	RchiOBHm_Chr2g0147861
rosa_laevigata	RLG00000020286
rosa_multiflora	Rmu_sc0000308.1_g000054
rosa_roxburghii	Rroxscaffold_2G00099260
rosa_rugosa	Rorug02G0402500
rosa_samantha	Rh2AG459400 Rh2BG472800 Rh2CG446800 Rh2DG481700
rosa_wichuraiana	Rw2G037580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	438
Acc16I	TGCGCA	1 cut(s)	1227
Acc36I	ACCTGC	1 cut(s)	331
AccB1I	GGYRCC	3 cut(s)	892, 1046, 1268
AccB7I	CCANNNNNTGG	1 cut(s)	1150
AccBSI	CCGCTC	1 cut(s)	1161
AccI	GTMKAC	1 cut(s)	39
AccII	CGCG	3 cut(s)	143, 287, 362
AclI	AACGTT	2 cut(s)	720, 1560
AclWI	GGATC	6 cut(s)	582, 612, 763, 1621, 1632, 1696
AcoI	YGGCCR	1 cut(s)	1677
AcsI	RAATTY	3 cut(s)	629, 659, 712
AcuI	CTGAAG	1 cut(s)	251
AcyI	GRCGYC	2 cut(s)	224, 435
AfaI	GTAC	3 cut(s)	186, 1184, 1595
AfiI	CCNNNNNNNGG	2 cut(s)	1150, 1616
AjiI	CACGTC	2 cut(s)	460, 552
AjnI	CCWGG	5 cut(s)	417, 1143, 1209, 1328, 1609
AjuI	GAANNNNNNNTTGG	4 cut(s)	1226, 1258, 1565, 1597
AluBI	AGCT	7 cut(s)	46, 331, 367, 517, 1035, 1298, 1367
AluI	AGCT	7 cut(s)	46, 331, 367, 517, 1035, 1298, 1367
Alw21I	GWGCWC	2 cut(s)	48, 563
Alw26I	GTCTC	5 cut(s)	89, 106, 287, 575, 1739
AlwI	GGATC	6 cut(s)	582, 612, 763, 1621, 1632, 1696
Ama87I	CYCGRG	5 cut(s)	259, 274, 290, 371, 743
AoxI	GGCC	7 cut(s)	57, 202, 445, 1469, 1607, 1641, 1677
ApeKI	GCWGC	5 cut(s)	376, 739, 802, 805, 1295
ApoI	RAATTY	3 cut(s)	629, 659, 712
ArsI	GACNNNNNNTTYG	2 cut(s)	1268, 1300
Asp700I	GAANNNNTTC	1 cut(s)	1349
AspLEI	GCGC	3 cut(s)	289, 829, 1228
AspS9I	GGNCC	6 cut(s)	203, 445, 1171, 1381, 1607, 1641
AsuC2I	CCSGG	7 cut(s)	76, 201, 314, 372, 373, 774, 789
AsuHPI	GGTGA	4 cut(s)	136, 160, 722, 1724
AvaI	CYCGRG	5 cut(s)	259, 274, 290, 371, 743
AvaII	GGWCC	2 cut(s)	1171, 1381
BanI	GGYRCC	3 cut(s)	892, 1046, 1268
BanII	GRGCYC	1 cut(s)	48
BbsI	GAAGAC	4 cut(s)	171, 462, 1010, 1016
Bbv12I	GWGCWC	2 cut(s)	48, 563
BbvI	GCAGC	5 cut(s)	363, 726, 789, 792, 1307
BccI	CCATC	4 cut(s)	842, 955, 992, 1196
BceAI	ACGGC	4 cut(s)	46, 840, 1060, 1061
BciT130I	CCWGG	5 cut(s)	419, 1145, 1211, 1330, 1611
BciVI	GTATCC	1 cut(s)	880
BclI	TGATCA	2 cut(s)	126, 150
BcnI	CCSGG	7 cut(s)	76, 201, 314, 372, 373, 774, 789
BcoDI	GTCTC	5 cut(s)	89, 106, 287, 575, 1739
BfaI	CTAG	1 cut(s)	431
BfmI	CTRYAG	5 cut(s)	338, 806, 1125, 1186, 1299
BfuAI	ACCTGC	1 cut(s)	331
BfuI	GTATCC	1 cut(s)	880
Bme18I	GGWCC	2 cut(s)	1171, 1381
BmeT110I	CYCGRG	5 cut(s)	259, 274, 290, 371, 743
BmgBI	CACGTC	2 cut(s)	460, 552
BmgT120I	GGNCC	6 cut(s)	203, 445, 1171, 1381, 1607, 1641
BmiI	GGNNCC	5 cut(s)	205, 894, 1048, 1270, 1383
BmsI	GCATC	3 cut(s)	288, 633, 1181
BpiI	GAAGAC	4 cut(s)	171, 462, 1010, 1016
BpmI	CTGGAG	1 cut(s)	440
Bpu10I	CCTNAGC	1 cut(s)	698
BpuEI	CTTGAG	2 cut(s)	1051, 1353
BpuMI	CCSGG	7 cut(s)	76, 201, 314, 372, 373, 774, 789
BsaBI	GATNNNNATC	1 cut(s)	501
BsaHI	GRCGYC	2 cut(s)	224, 435
BsaJI	CCNNGG	9 cut(s)	105, 199, 371, 492, 788, 1328, 1472, 1491, 1705
BsaWI	WCCGGW	2 cut(s)	724, 732
Bsc4I	CCNNNNNNNGG	2 cut(s)	1150, 1616
Bse118I	RCCGGY	4 cut(s)	379, 1265, 1643, 1723
Bse1I	ACTGG	2 cut(s)	1348, 1402
Bse3DI	GCAATG	1 cut(s)	1297
Bse8I	GATNNNNATC	1 cut(s)	501
BseBI	CCWGG	5 cut(s)	419, 1145, 1211, 1330, 1611
BseDI	CCNNGG	9 cut(s)	105, 199, 371, 492, 788, 1328, 1472, 1491, 1705
BseGI	GGATG	4 cut(s)	90, 502, 853, 1320
BseJI	GATNNNNATC	1 cut(s)	501
BseLI	CCNNNNNNNGG	2 cut(s)	1150, 1616
BseMI	GCAATG	1 cut(s)	1297
BseMII	CTCAG	2 cut(s)	111, 689
BseNI	ACTGG	2 cut(s)	1348, 1402
BseXI	GCAGC	5 cut(s)	363, 726, 789, 792, 1307
BsgI	GTGCAG	1 cut(s)	1438
Bsh1236I	CGCG	3 cut(s)	143, 287, 362
BshFI	GGCC	7 cut(s)	59, 204, 447, 1471, 1609, 1643, 1679
BshNI	GGYRCC	3 cut(s)	892, 1046, 1268
BsiHKAI	GWGCWC	2 cut(s)	48, 563
BsiHKCI	CYCGRG	5 cut(s)	259, 274, 290, 371, 743
BslFI	GGGAC	1 cut(s)	865
BslI	CCNNNNNNNGG	2 cut(s)	1150, 1616
BsmAI	GTCTC	5 cut(s)	89, 106, 287, 575, 1739
BsmBI	CGTCTC	1 cut(s)	89
BsmFI	GGGAC	1 cut(s)	865
BsmI	GAATGC	2 cut(s)	1154, 1228
BsnI	GGCC	7 cut(s)	59, 204, 447, 1471, 1609, 1643, 1679
BsoBI	CYCGRG	5 cut(s)	259, 274, 290, 371, 743
Bsp1286I	GDGCHC	2 cut(s)	48, 563
Bsp19I	CCATGG	1 cut(s)	492
BspANI	GGCC	7 cut(s)	59, 204, 447, 1471, 1609, 1643, 1679
BspCNI	CTCAG	2 cut(s)	110, 690
BspFNI	CGCG	3 cut(s)	143, 287, 362
BspLI	GGNNCC	5 cut(s)	205, 894, 1048, 1270, 1383
BspMAI	CTGCAG	3 cut(s)	342, 810, 1129
BspMI	ACCTGC	1 cut(s)	331
BspPI	GGATC	6 cut(s)	582, 612, 763, 1621, 1632, 1696
BspQI	GCTCTTC	2 cut(s)	45, 896
BspT107I	GGYRCC	3 cut(s)	892, 1046, 1268
BsrBI	CCGCTC	1 cut(s)	1161
BsrDI	GCAATG	1 cut(s)	1297
BsrFI	RCCGGY	4 cut(s)	379, 1265, 1643, 1723
BsrI	ACTGG	2 cut(s)	1348, 1402
BssAI	RCCGGY	4 cut(s)	379, 1265, 1643, 1723
BssECI	CCNNGG	9 cut(s)	105, 199, 371, 492, 788, 1328, 1472, 1491, 1705
BssNI	GRCGYC	2 cut(s)	224, 435
BssT1I	CCWWGG	3 cut(s)	492, 1472, 1705
Bst2UI	CCWGG	5 cut(s)	419, 1145, 1211, 1330, 1611
Bst4CI	ACNGT	7 cut(s)	109, 892, 1015, 1439, 1495, 1604, 1690
Bst6I	CTCTTC	2 cut(s)	45, 896
BstACI	GRCGYC	2 cut(s)	224, 435
BstC8I	GCNNGC	3 cut(s)	57, 381, 1725
BstDEI	CTNAG	2 cut(s)	97, 698
BstDSI	CCRYGG	3 cut(s)	105, 492, 1491
BstEII	GGTNACC	1 cut(s)	1552
BstENI	CCTNNNNNAGG	1 cut(s)	1614
BstF5I	GGATG	4 cut(s)	90, 502, 853, 1320
BstFNI	CGCG	3 cut(s)	143, 287, 362
BstHHI	GCGC	3 cut(s)	289, 829, 1228
BstMAI	GTCTC	5 cut(s)	89, 106, 287, 575, 1739
BstMWI	GCNNNNNNNGC	7 cut(s)	52, 337, 353, 373, 686, 1032, 1073
BstNI	CCWGG	5 cut(s)	419, 1145, 1211, 1330, 1611
BstPI	GGTNACC	1 cut(s)	1552
BstSFI	CTRYAG	5 cut(s)	338, 806, 1125, 1186, 1299
BstUI	CGCG	3 cut(s)	143, 287, 362
BstV1I	GCAGC	5 cut(s)	363, 726, 789, 792, 1307
BstV2I	GAAGAC	4 cut(s)	171, 462, 1010, 1016
BstX2I	RGATCY	1 cut(s)	604
BstXI	CCANNNNNNTGG	2 cut(s)	1539, 1604
BstYI	RGATCY	1 cut(s)	604
BsuI	GTATCC	1 cut(s)	880
BsuRI	GGCC	7 cut(s)	59, 204, 447, 1471, 1609, 1643, 1679
BtgI	CCRYGG	3 cut(s)	105, 492, 1491
BtgZI	GCGATG	2 cut(s)	385, 656
BtrI	CACGTC	2 cut(s)	460, 552
BtsCI	GGATG	4 cut(s)	90, 502, 853, 1320
BtsI	GCAGTG	3 cut(s)	1122, 1155, 1446
BtsIMutI	CAGTG	5 cut(s)	1122, 1155, 1395, 1446, 1609
BveI	ACCTGC	1 cut(s)	331
Cac8I	GCNNGC	3 cut(s)	57, 381, 1725
CfoI	GCGC	3 cut(s)	289, 829, 1228
Cfr10I	RCCGGY	4 cut(s)	379, 1265, 1643, 1723
Cfr13I	GGNCC	6 cut(s)	203, 445, 1171, 1381, 1607, 1641
Cfr9I	CCCGGG	1 cut(s)	371
CseI	GACGC	2 cut(s)	71, 213
Csp6I	GTAC	3 cut(s)	185, 1183, 1594
CspCI	CAANNNNNGTGG	2 cut(s)	1716, 1751
CviAII	CATG	3 cut(s)	493, 926, 1501
CviQI	GTAC	3 cut(s)	185, 1183, 1594
DdeI	CTNAG	2 cut(s)	97, 698
EaeI	YGGCCR	1 cut(s)	1677
Eam1104I	CTCTTC	2 cut(s)	45, 896
EarI	CTCTTC	2 cut(s)	45, 896
EciI	GGCGGA	2 cut(s)	514, 617
Ecl136II	GAGCTC	1 cut(s)	46
Eco130I	CCWWGG	3 cut(s)	492, 1472, 1705
Eco147I	AGGCCT	2 cut(s)	59, 1471
Eco24I	GRGCYC	1 cut(s)	48
Eco47I	GGWCC	2 cut(s)	1171, 1381
Eco53kI	GAGCTC	1 cut(s)	46
Eco57I	CTGAAG	1 cut(s)	251
Eco88I	CYCGRG	5 cut(s)	259, 274, 290, 371, 743
Eco91I	GGTNACC	1 cut(s)	1552
EcoICRI	GAGCTC	1 cut(s)	46
EcoNI	CCTNNNNNAGG	1 cut(s)	1614
EcoO109I	RGGNCCY	2 cut(s)	445, 1381
EcoO65I	GGTNACC	1 cut(s)	1552
EcoRI	GAATTC	1 cut(s)	629
EcoRII	CCWGG	5 cut(s)	417, 1143, 1209, 1328, 1609
EcoT14I	CCWWGG	3 cut(s)	492, 1472, 1705
EcoT38I	GRGCYC	1 cut(s)	48
ErhI	CCWWGG	3 cut(s)	492, 1472, 1705
Esp3I	CGTCTC	1 cut(s)	89
FaeI	CATG	3 cut(s)	496, 929, 1504
FaqI	GGGAC	1 cut(s)	865
FatI	CATG	3 cut(s)	492, 925, 1500
FauI	CCCGC	1 cut(s)	136
FbaI	TGATCA	2 cut(s)	126, 150
FblI	GTMKAC	1 cut(s)	39
FokI	GGATG	4 cut(s)	77, 509, 860, 1307
FriOI	GRGCYC	1 cut(s)	48
FspAI	RTGCGCAY	1 cut(s)	1227
FspBI	CTAG	1 cut(s)	431
FspI	TGCGCA	1 cut(s)	1227
GlaI	GCGC	3 cut(s)	288, 828, 1227
GsuI	CTGGAG	1 cut(s)	440
HaeIII	GGCC	7 cut(s)	59, 204, 447, 1471, 1609, 1643, 1679
HgaI	GACGC	2 cut(s)	71, 213
HhaI	GCGC	3 cut(s)	289, 829, 1228
Hin1I	GRCGYC	2 cut(s)	224, 435
Hin1II	CATG	3 cut(s)	496, 929, 1504
Hin6I	GCGC	3 cut(s)	287, 827, 1226
HinP1I	GCGC	3 cut(s)	287, 827, 1226
HincII	GTYRAC	3 cut(s)	40, 466, 1564
HindII	GTYRAC	3 cut(s)	40, 466, 1564
HindIII	AAGCTT	2 cut(s)	365, 515
HphI	GGTGA	4 cut(s)	136, 160, 722, 1724
Hpy166II	GTNNAC	7 cut(s)	40, 466, 730, 1042, 1183, 1564, 1686
Hpy188I	TCNGA	8 cut(s)	396, 752, 760, 921, 995, 1171, 1392, 1510
Hpy8I	GTNNAC	7 cut(s)	40, 466, 730, 1042, 1183, 1564, 1686
Hpy99I	CGWCG	4 cut(s)	44, 178, 229, 826
HpyAV	CCTTC	8 cut(s)	28, 70, 226, 713, 838, 1066, 1471, 1537
HpyCH4III	ACNGT	7 cut(s)	109, 892, 1015, 1439, 1495, 1604, 1690
HpyCH4IV	ACGT	5 cut(s)	435, 459, 551, 720, 1560
HpyCH4V	TGCA	9 cut(s)	340, 646, 808, 1127, 1295, 1317, 1419, 1504, 1589
HpyF10VI	GCNNNNNNNGC	7 cut(s)	52, 337, 353, 373, 686, 1032, 1073
HpyF3I	CTNAG	2 cut(s)	97, 698
HpySE526I	ACGT	5 cut(s)	435, 459, 551, 720, 1560
Hsp92I	GRCGYC	2 cut(s)	224, 435
Hsp92II	CATG	3 cut(s)	496, 929, 1504
HspAI	GCGC	3 cut(s)	287, 827, 1226
KroI	GCCGGC	2 cut(s)	379, 1723
KroNI	GCCGGC	2 cut(s)	381, 1725
Ksp22I	TGATCA	2 cut(s)	126, 150
LguI	GCTCTTC	2 cut(s)	45, 896
LmnI	GCTCC	1 cut(s)	1130
Lsp1109I	GCAGC	5 cut(s)	363, 726, 789, 792, 1307
LweI	GCATC	3 cut(s)	288, 633, 1181
MaeI	CTAG	1 cut(s)	431
MaeII	ACGT	5 cut(s)	435, 459, 551, 720, 1560
MaeIII	GTNAC	6 cut(s)	247, 1078, 1256, 1552, 1646, 1690
MbiI	CCGCTC	1 cut(s)	1161
MboII	GAAGA	7 cut(s)	62, 171, 467, 913, 1015, 1021, 1108
MflI	RGATCY	1 cut(s)	604
MhlI	GDGCHC	2 cut(s)	48, 563
MluCI	AATT	5 cut(s)	209, 629, 659, 712, 1241
MlyI	GAGTC	3 cut(s)	208, 473, 587
MmeI	TCCRAC	3 cut(s)	730, 758, 1257
MroNI	GCCGGC	2 cut(s)	379, 1723
MroXI	GAANNNNTTC	1 cut(s)	1349
MseI	TTAA	2 cut(s)	864, 1635
MslI	CAYNNNNRTG	1 cut(s)	497
MspA1I	CMGCKG	1 cut(s)	1298
Mva1269I	GAATGC	2 cut(s)	1154, 1228
MvaI	CCWGG	5 cut(s)	419, 1145, 1211, 1330, 1611
MvnI	CGCG	3 cut(s)	143, 287, 362
MwoI	GCNNNNNNNGC	7 cut(s)	52, 337, 353, 373, 686, 1032, 1073
NaeI	GCCGGC	2 cut(s)	381, 1725
NciI	CCSGG	7 cut(s)	76, 201, 314, 372, 373, 774, 789
NcoI	CCATGG	1 cut(s)	492
NgoMIV	GCCGGC	2 cut(s)	379, 1723
NlaIII	CATG	3 cut(s)	496, 929, 1504
NlaIV	GGNNCC	5 cut(s)	205, 894, 1048, 1270, 1383
NmuCI	GTSAC	3 cut(s)	247, 1078, 1256
NsbI	TGCGCA	1 cut(s)	1227
PaeR7I	CTCGAG	2 cut(s)	274, 290
PceI	AGGCCT	2 cut(s)	59, 1471
PciSI	GCTCTTC	2 cut(s)	45, 896
PcsI	WCGNNNNNNNCGW	3 cut(s)	36, 45, 272
PctI	GAATGC	2 cut(s)	1154, 1228
PdiI	GCCGGC	2 cut(s)	381, 1725
PdmI	GAANNNNTTC	1 cut(s)	1349
PfeI	GAWTC	6 cut(s)	502, 871, 916, 922, 1113, 1387
PflFI	GACNNNGTC	1 cut(s)	8
PflMI	CCANNNNNTGG	1 cut(s)	1150
PfoI	TCCNGGA	1 cut(s)	1209
PleI	GAGTC	3 cut(s)	208, 473, 587
PpsI	GAGTC	3 cut(s)	208, 473, 587
PpuMI	RGGWCCY	1 cut(s)	1381
Psp124BI	GAGCTC	1 cut(s)	48
Psp1406I	AACGTT	2 cut(s)	720, 1560
Psp5II	RGGWCCY	1 cut(s)	1381
Psp6I	CCWGG	5 cut(s)	417, 1143, 1209, 1328, 1609
PspEI	GGTNACC	1 cut(s)	1552
PspGI	CCWGG	5 cut(s)	417, 1143, 1209, 1328, 1609
PspN4I	GGNNCC	5 cut(s)	205, 894, 1048, 1270, 1383
PspPI	GGNCC	6 cut(s)	203, 445, 1171, 1381, 1607, 1641
PspPPI	RGGWCCY	1 cut(s)	1381
PstI	CTGCAG	3 cut(s)	342, 810, 1129
PsuI	RGATCY	1 cut(s)	604
PsyI	GACNNNGTC	1 cut(s)	8
PvuII	CAGCTG	1 cut(s)	1298
RsaI	GTAC	3 cut(s)	186, 1184, 1595
RsaNI	GTAC	3 cut(s)	185, 1183, 1594
RseI	CAYNNNNRTG	1 cut(s)	497
SacI	GAGCTC	1 cut(s)	48
SalI	GTCGAC	1 cut(s)	38
SapI	GCTCTTC	2 cut(s)	45, 896
SaqAI	TTAA	2 cut(s)	864, 1635
Sau96I	GGNCC	6 cut(s)	203, 445, 1171, 1381, 1607, 1641
SchI	GAGTC	3 cut(s)	208, 473, 587
SduI	GDGCHC	2 cut(s)	48, 563
SfaNI	GCATC	3 cut(s)	288, 633, 1181
SfcI	CTRYAG	5 cut(s)	338, 806, 1125, 1186, 1299
Sfr274I	CTCGAG	2 cut(s)	274, 290
SinI	GGWCC	2 cut(s)	1171, 1381
SlaI	CTCGAG	2 cut(s)	274, 290
SmaI	CCCGGG	1 cut(s)	373
SmiMI	CAYNNNNRTG	1 cut(s)	497
SmlI	CTYRAG	4 cut(s)	274, 290, 1030, 1368
SmoI	CTYRAG	4 cut(s)	274, 290, 1030, 1368
Sse9I	AATT	5 cut(s)	209, 629, 659, 712, 1241
SseBI	AGGCCT	2 cut(s)	59, 1471
SspMI	CTAG	1 cut(s)	431
SstI	GAGCTC	1 cut(s)	48
StuI	AGGCCT	2 cut(s)	59, 1471
StyI	CCWWGG	3 cut(s)	492, 1472, 1705
TaaI	ACNGT	7 cut(s)	109, 892, 1015, 1439, 1495, 1604, 1690
TaiI	ACGT	5 cut(s)	438, 462, 554, 723, 1563
TaqI	TCGA	8 cut(s)	39, 48, 194, 275, 291, 299, 614, 665
TaqII	GACCGA	1 cut(s)	380
TasI	AATT	5 cut(s)	209, 629, 659, 712, 1241
TatI	WGTACW	1 cut(s)	1182
TauI	GCSGC	7 cut(s)	224, 246, 359, 362, 530, 641, 1679
TfiI	GAWTC	6 cut(s)	502, 871, 916, 922, 1113, 1387
Tru1I	TTAA	2 cut(s)	864, 1635
Tru9I	TTAA	2 cut(s)	864, 1635
TscAI	CASTG	5 cut(s)	1129, 1155, 1402, 1453, 1609
TseFI	GTSAC	3 cut(s)	247, 1078, 1256
TseI	GCWGC	5 cut(s)	376, 739, 802, 805, 1295
Tsp45I	GTSAC	3 cut(s)	247, 1078, 1256
TspDTI	ATGAA	3 cut(s)	914, 1084, 1270
TspGWI	ACGGA	1 cut(s)	587
TspMI	CCCGGG	1 cut(s)	371
TspRI	CASTG	5 cut(s)	1129, 1155, 1402, 1453, 1609
Tth111I	GACNNNGTC	1 cut(s)	8
Van91I	CCANNNNNTGG	1 cut(s)	1150
VpaK11BI	GGWCC	2 cut(s)	1171, 1381
XagI	CCTNNNNNAGG	1 cut(s)	1614
XapI	RAATTY	3 cut(s)	629, 659, 712
XbaI	TCTAGA	1 cut(s)	430
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1336
XhoI	CTCGAG	2 cut(s)	274, 290
XmaI	CCCGGG	1 cut(s)	371
XmiI	GTMKAC	1 cut(s)	39
XmnI	GAANNNNTTC	1 cut(s)	1349
XspI	CTAG	1 cut(s)	431
ZraI	GACGTC	1 cut(s)	436

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.