Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr2A

Orthogroup ID

OG0015780

Physical Location & Seq

Genomic Coordinates Reverse (-)

67545724 .. 67549694

Gene Structure 3971 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh2AG459400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1755 bp

ATGAGTTCCTTTAAGGGCTACGGCAAAGTCGACGAGCTCGAAGAGCAAGCCTTCCGTCTCAAAACCCGCAAGCGCTTGCTCATCGTCACCGTCTCCGTCATTGTCCTCTTGGCCGTCATCATCGGCGCCGTCACCGGCACCGTCATCCACAACCGCAACAGCTCCTCTTCGCCGTCGCCGAACACGGAACCCGCCACTCAGTTGACTCCCTCCGCTTCCTTAAAAGCCGTCTGCGGCGTCACCCAGTACCCCAACTCGTGCTTCTCGAGCATCTCTTCTCTCAAAAACTCCAACACCACCGACCCGGAAGCGCTGTTCAAGCTGTCCCTGCATGTCGCCATTAACGAGGCCTCGAAGCTCAAAGACTTGCCTTCAACAATAAAGATCGGAAATGATAGCAAACTCGAAAGCGCTATTAAGGTCTGCAAGACCGTTTTCGATGATGCTGTGGATAAGCTTAACGATTCGCTCTCTGCAATGGATGTCAGTAGTTCCGGCGAGAAGCTGTTGTCCGAGGCGAAGATCAATGACCTGAAGACGTGGATCAGCGCCACCATGACTGACCAAGAGACCTGCCTGGACTCTCTTGAAGACATCAATTCGACTTATGTTCAGGAATTCAGGGATGCTATGCAGAACTCCACAGAATACGCCAGCAACAGCCTGGCCATTGTGGCGAAAATTCTGGGGTTATTGTCCAATTTGAACATTCCGGTTCACCGGAGATTGCTCGGGTACGGAGGAGAAAGATCCTCTGTACCCGAGTTCCCGGGCTGGGTCAGCCCGGGAGACAGGAGGCTGCTGCAGGAGCAGAACCTGACCGCGCACGTGGTTGTGGCGAAGGATGGGACTGGGGATTTTAAGACCATTCAGGAGGCTGTGAATGCTGTGCCCAAGAAGAACTTGACCAGGTTTGTGATATATGTGAAGCAAGGGACGTACGAAGAAAATGTGATCATGGACAAGAGCACATGGAATGTGATGATGTATGGAGATGGCAAGACCAAGACCATTGTTTCTGGGAAGTTGAACTATGTGGATGGGACTGCAACTTTCAACACAGCCACTTTTGCTGTAGCAGGAAGAGGGTTCATTGCGAAAGATATAGGGTTCATTAACACTGCTGGAGCTGCAAAGCACCAAGCGGTGGCATTCCGATCTGGTTCCGACCAATCTGTGTTCTACAGATGCTCATTTGATGCATTCCAGGACACTCTATATGCTCACTCAAATCGACAATTCTATCGTGACTGTGACATCACTGGTACCATTGACTTCATGTTTGGCAATGCAGCTGTGGTGTTCCAGAACTGTAACATCAAGCCGAGGCAACCAATGCCAAACCAGTTCAACACAATCACTGCTCAGGGAAAGAAGGACCCCAATCAAAACACTGGCATTTCAATTCAGAAGTGCACAATCTCTCCACTCGAAAGCAACATGACGGCTCCAACATATCTTGGTAGACCTTGGAAGAACTTTTCCACCACGGTTTTCTTAGAATCCAGTATTGATTCGTTCTTGAACCCATCGGGGTGGACAGAATGGATTCCCAATGTTGATCCACCTAACACCATATTTTACGCAGAATACCAAAATAGTGGACCAGGATCGAGTGTGGATAAAAGGGTTACATGGGCTGGTTACAGGCCCTCTCTCACAGATACTGAAGCAGCCAAGTTCACAGTTGAGTCCTTCATCCAAGGTAATGAATGGTTGCCTCAGACTAACGTGAATTTTGATTCATCCCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0008150 GO:0009506 GO:0012505 GO:0016020 GO:0016787 GO:0016788 GO:0030054 GO:0030234 GO:0030599 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0043086 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044422 GO:0044424 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046910 GO:0050790 GO:0051722 GO:0051723 GO:0052689 GO:0055044 GO:0065007 GO:0065009 GO:0071944 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098805 GO:0140096

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

584

Amino Acids

64.11

Weight (kDa)

6.16

Isoelectric Point (pI)

32.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	73 - 224	2.3e-30	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	276 - 570	7.8e-141	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015780)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53840 AT3G14300
fragaria_vesca	FvH4_6g35820
malus_domestica	MD09G1172900.v1.1 MD17G1154400.v1.1
rosa_chinensis	RchiOBHm_Chr2g0147861
rosa_laevigata	RLG00000020286
rosa_multiflora	Rmu_sc0000308.1_g000054
rosa_roxburghii	Rroxscaffold_2G00099260
rosa_rugosa	Rorug02G0402500
rosa_samantha	Rh2AG459400 Rh2BG472800 Rh2CG446800 Rh2DG481700
rosa_wichuraiana	Rw2G037580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	581
Acc65I	GGTACC	1 cut(s)	1265
AccB1I	GGYRCC	3 cut(s)	125, 137, 1265
AccB7I	CCANNNNNTGG	1 cut(s)	1147
AccI	GTMKAC	2 cut(s)	30, 1465
AccII	CGCG	1 cut(s)	824
AciI	CCGC	7 cut(s)	67, 154, 192, 213, 234, 822, 1145
AclWI	GGATC	4 cut(s)	551, 744, 1556, 1618
AcoI	YGGCCR	2 cut(s)	111, 666
AcsI	RAATTY	3 cut(s)	617, 681, 1735
AcuI	CTGAAG	2 cut(s)	554, 1689
AcvI	CACGTG	1 cut(s)	829
AcyI	GRCGYC	2 cut(s)	126, 237
AfaI	GTAC	5 cut(s)	248, 737, 759, 941, 1267
AfeI	AGCGCT	3 cut(s)	74, 312, 412
AfiI	CCNNNNNNNGG	2 cut(s)	775, 1147
AgsI	TTSAA	9 cut(s)	319, 375, 590, 706, 1030, 1057, 1351, 1404, 1525
AjiI	CACGTC	1 cut(s)	540
AjnI	CCWGG	5 cut(s)	576, 663, 908, 1206, 1606
AjuI	GAANNNNNNNTTGG	2 cut(s)	245, 277
AloI	GAACNNNNNNTCC	2 cut(s)	299, 331
AluBI	AGCT	8 cut(s)	37, 162, 322, 358, 457, 505, 1130, 1295
AluI	AGCT	8 cut(s)	37, 162, 322, 358, 457, 505, 1130, 1295
Alw21I	GWGCWC	3 cut(s)	39, 971, 1418
Alw26I	GTCTC	4 cut(s)	62, 97, 563, 783
Alw44I	GTGCAC	1 cut(s)	1414
AlwI	GGATC	4 cut(s)	551, 744, 1556, 1618
AlwNI	CAGNNNCTG	2 cut(s)	817, 1667
Ama87I	CYCGRG	5 cut(s)	265, 731, 761, 769, 784
Aor51HI	AGCGCT	3 cut(s)	74, 312, 412
AoxI	GGCC	4 cut(s)	111, 348, 666, 1649
ApaLI	GTGCAC	1 cut(s)	1414
ApeKI	GCWGC	5 cut(s)	799, 802, 1130, 1292, 1673
ApoI	RAATTY	3 cut(s)	617, 681, 1735
ArsI	GACNNNNNNTTYG	2 cut(s)	1265, 1297
Asp700I	GAANNNNTTC	1 cut(s)	1548
Asp718I	GGTACC	1 cut(s)	1265
AspLEI	GCGC	6 cut(s)	75, 128, 313, 413, 551, 826
AspS9I	GGNCC	3 cut(s)	1378, 1604, 1650
AsuC2I	CCSGG	5 cut(s)	305, 770, 771, 785, 786
AsuHPI	GGTGA	4 cut(s)	79, 124, 232, 710
AvaI	CYCGRG	5 cut(s)	265, 731, 761, 769, 784
AvaII	GGWCC	2 cut(s)	1378, 1604
BaeGI	GKGCMC	2 cut(s)	894, 1418
BalI	TGGCCA	1 cut(s)	668
BanI	GGYRCC	3 cut(s)	125, 137, 1265
BanII	GRGCYC	1 cut(s)	39
BauI	CACGAG	1 cut(s)	256
BbrPI	CACGTG	1 cut(s)	829
BbsI	GAAGAC	2 cut(s)	542, 597
Bbv12I	GWGCWC	3 cut(s)	39, 971, 1418
BbvI	GCAGC	5 cut(s)	786, 789, 1117, 1304, 1685
BccI	CCATC	4 cut(s)	839, 989, 1034, 1537
BceAI	ACGGC	6 cut(s)	37, 98, 113, 157, 212, 1461
BciT130I	CCWGG	5 cut(s)	578, 665, 910, 1208, 1608
BclI	TGATCA	1 cut(s)	954
BcnI	CCSGG	5 cut(s)	305, 770, 771, 785, 786
BcoDI	GTCTC	4 cut(s)	62, 97, 563, 783
BfmI	CTRYAG	3 cut(s)	803, 1074, 1183
BfoI	RGCGCY	5 cut(s)	76, 129, 314, 414, 552
BfuAI	ACCTGC	1 cut(s)	581
BglI	GCCNNNNNGGC	1 cut(s)	674
BisI	GCNGC	6 cut(s)	235, 800, 803, 1131, 1293, 1674
BlsI	GCNGC	6 cut(s)	236, 801, 804, 1132, 1294, 1675
Bme18I	GGWCC	2 cut(s)	1378, 1604
BmeT110I	CYCGRG	5 cut(s)	265, 731, 761, 769, 784
BmgBI	CACGTC	1 cut(s)	540
BmgT120I	GGNCC	3 cut(s)	1378, 1604, 1650
BmiI	GGNNCC	7 cut(s)	127, 139, 189, 1165, 1267, 1380, 1449
BmrI	ACTGGG	2 cut(s)	238, 861
BmsI	GCATC	5 cut(s)	279, 433, 616, 1178, 1189
BmuI	ACTGGG	2 cut(s)	238, 861
BpiI	GAAGAC	2 cut(s)	542, 597
BpmI	CTGGAG	1 cut(s)	1146
Bpu10I	CCTNAGC	1 cut(s)	1365
BpuMI	CCSGG	5 cut(s)	305, 770, 771, 785, 786
BsaAI	YACGTR	1 cut(s)	829
BsaHI	GRCGYC	2 cut(s)	126, 237
BsaI	GGTCTC	1 cut(s)	563
BsaJI	CCNNGG	7 cut(s)	513, 769, 784, 1325, 1469, 1488, 1702
BsaWI	WCCGGW	2 cut(s)	712, 720
BsaXI	ACNNNNNCTCC	2 cut(s)	1408, 1438
Bsc4I	CCNNNNNNNGG	2 cut(s)	775, 1147
Bse118I	RCCGGY	1 cut(s)	134
Bse1I	ACTGG	6 cut(s)	244, 856, 1267, 1345, 1399, 1506
Bse3DI	GCAATG	3 cut(s)	483, 1092, 1294
BseBI	CCWGG	5 cut(s)	578, 665, 910, 1208, 1608
BseDI	CCNNGG	7 cut(s)	513, 769, 784, 1325, 1469, 1488, 1702
BseGI	GGATG	7 cut(s)	144, 487, 631, 850, 1045, 1698, 1745
BseLI	CCNNNNNNNGG	2 cut(s)	775, 1147
BseMI	GCAATG	3 cut(s)	483, 1092, 1294
BseMII	CTCAG	3 cut(s)	212, 1379, 1736
BseNI	ACTGG	6 cut(s)	244, 856, 1267, 1345, 1399, 1506
BseRI	GAGGAG	2 cut(s)	154, 756
BseSI	GKGCMC	2 cut(s)	894, 1418
BseXI	GCAGC	5 cut(s)	786, 789, 1117, 1304, 1685
BseYI	CCCAGC	1 cut(s)	774
Bsh1236I	CGCG	1 cut(s)	824
BshFI	GGCC	4 cut(s)	113, 350, 668, 1651
BshNI	GGYRCC	3 cut(s)	125, 137, 1265
BsiHKAI	GWGCWC	3 cut(s)	39, 971, 1418
BsiHKCI	CYCGRG	5 cut(s)	265, 731, 761, 769, 784
BsiSI	CCGG	7 cut(s)	135, 305, 495, 713, 721, 770, 785
BsiWI	CGTACG	1 cut(s)	939
BslFI	GGGAC	4 cut(s)	310, 862, 949, 1057
BslI	CCNNNNNNNGG	2 cut(s)	775, 1147
BsmAI	GTCTC	4 cut(s)	62, 97, 563, 783
BsmBI	CGTCTC	2 cut(s)	62, 97
BsmFI	GGGAC	4 cut(s)	310, 862, 949, 1057
BsmI	GAATGC	3 cut(s)	889, 1151, 1202
BsnI	GGCC	4 cut(s)	113, 350, 668, 1651
Bso31I	GGTCTC	1 cut(s)	563
BsoBI	CYCGRG	5 cut(s)	265, 731, 761, 769, 784
Bsp1286I	GDGCHC	4 cut(s)	39, 894, 971, 1418
Bsp143I	GATC	8 cut(s)	384, 522, 543, 749, 954, 1157, 1561, 1610
BspACI	CCGC	7 cut(s)	67, 154, 192, 213, 234, 822, 1145
BspANI	GGCC	4 cut(s)	113, 350, 668, 1651
BspCNI	CTCAG	3 cut(s)	211, 1378, 1735
BspFNI	CGCG	1 cut(s)	824
BspLI	GGNNCC	7 cut(s)	127, 139, 189, 1165, 1267, 1380, 1449
BspMAI	CTGCAG	1 cut(s)	807
BspMI	ACCTGC	1 cut(s)	581
BspPI	GGATC	4 cut(s)	551, 744, 1556, 1618
BspQI	GCTCTTC	1 cut(s)	36
BspT107I	GGYRCC	3 cut(s)	125, 137, 1265
BspTNI	GGTCTC	1 cut(s)	563
BsrDI	GCAATG	3 cut(s)	483, 1092, 1294
BsrFI	RCCGGY	1 cut(s)	134
BsrI	ACTGG	6 cut(s)	244, 856, 1267, 1345, 1399, 1506
BssAI	RCCGGY	1 cut(s)	134
BssECI	CCNNGG	7 cut(s)	513, 769, 784, 1325, 1469, 1488, 1702
BssMI	GATC	8 cut(s)	384, 522, 543, 749, 954, 1157, 1561, 1610
BssNI	GRCGYC	2 cut(s)	126, 237
BssSI	CACGAG	1 cut(s)	256
BssT1I	CCWWGG	2 cut(s)	1469, 1702
Bst2BI	CACGAG	1 cut(s)	256
Bst2UI	CCWGG	5 cut(s)	578, 665, 910, 1208, 1608
Bst4CI	ACNGT	7 cut(s)	91, 142, 433, 1253, 1313, 1492, 1687
Bst6I	CTCTTC	4 cut(s)	36, 172, 280, 1078
BstACI	GRCGYC	2 cut(s)	126, 237
BstAPI	GCANNNNNTGC	1 cut(s)	1336
BstBAI	YACGTR	1 cut(s)	829
BstC8I	GCNNGC	4 cut(s)	48, 71, 77, 655
BstDEI	CTNAG	4 cut(s)	198, 1365, 1498, 1722
BstDSI	CCRYGG	1 cut(s)	1488
BstF5I	GGATG	7 cut(s)	144, 487, 631, 850, 1045, 1698, 1745
BstFNI	CGCG	1 cut(s)	824
BstH2I	RGCGCY	5 cut(s)	76, 129, 314, 414, 552
BstHHI	GCGC	6 cut(s)	75, 128, 313, 413, 551, 826
BstKTI	GATC	8 cut(s)	387, 525, 546, 752, 957, 1160, 1564, 1613
BstMAI	GTCTC	4 cut(s)	62, 97, 563, 783
BstMBI	GATC	8 cut(s)	384, 522, 543, 749, 954, 1157, 1561, 1610
BstNI	CCWGG	5 cut(s)	578, 665, 910, 1208, 1608
BstNSI	RCATGY	1 cut(s)	335
BstSFI	CTRYAG	3 cut(s)	803, 1074, 1183
BstSLI	GKGCMC	2 cut(s)	894, 1418
BstUI	CGCG	1 cut(s)	824
BstV1I	GCAGC	5 cut(s)	786, 789, 1117, 1304, 1685
BstV2I	GAAGAC	2 cut(s)	542, 597
BstX2I	RGATCY	1 cut(s)	749
BstXI	CCANNNNNNTGG	2 cut(s)	1536, 1601
BstYI	RGATCY	1 cut(s)	749
BsuRI	GGCC	4 cut(s)	113, 350, 668, 1651
BtgI	CCRYGG	1 cut(s)	1488
BtrI	CACGTC	1 cut(s)	540
BtsCI	GGATG	7 cut(s)	144, 487, 631, 850, 1045, 1698, 1745
BtsI	GCAGTG	2 cut(s)	1119, 1359
BtsIMutI	CAGTG	4 cut(s)	1119, 1260, 1359, 1392
BveI	ACCTGC	1 cut(s)	581
Cac8I	GCNNGC	4 cut(s)	48, 71, 77, 655
CaiI	CAGNNNCTG	2 cut(s)	817, 1667
CfoI	GCGC	6 cut(s)	75, 128, 313, 413, 551, 826
Cfr10I	RCCGGY	1 cut(s)	134
Cfr13I	GGNCC	3 cut(s)	1378, 1604, 1650
Cfr9I	CCCGGG	2 cut(s)	769, 784
CseI	GACGC	1 cut(s)	226
CsiI	ACCWGGT	1 cut(s)	908
Csp6I	GTAC	5 cut(s)	247, 736, 758, 940, 1266
CviAII	CATG	7 cut(s)	332, 556, 958, 972, 1279, 1441, 1635
CviQI	GTAC	5 cut(s)	247, 736, 758, 940, 1266
DdeI	CTNAG	4 cut(s)	198, 1365, 1498, 1722
DinI	GGCGCC	1 cut(s)	127
DpnI	GATC	8 cut(s)	386, 524, 545, 751, 956, 1159, 1563, 1612
DpnII	GATC	8 cut(s)	384, 522, 543, 749, 954, 1157, 1561, 1610
EaeI	YGGCCR	2 cut(s)	111, 666
Eam1104I	CTCTTC	4 cut(s)	36, 172, 280, 1078
EarI	CTCTTC	4 cut(s)	36, 172, 280, 1078
Ecl136II	GAGCTC	1 cut(s)	37
Eco130I	CCWWGG	2 cut(s)	1469, 1702
Eco147I	AGGCCT	1 cut(s)	350
Eco24I	GRGCYC	1 cut(s)	39
Eco31I	GGTCTC	1 cut(s)	563
Eco47I	GGWCC	2 cut(s)	1378, 1604
Eco47III	AGCGCT	3 cut(s)	74, 312, 412
Eco53kI	GAGCTC	1 cut(s)	37
Eco57I	CTGAAG	2 cut(s)	554, 1689
Eco72I	CACGTG	1 cut(s)	829
Eco88I	CYCGRG	5 cut(s)	265, 731, 761, 769, 784
EcoICRI	GAGCTC	1 cut(s)	37
EcoO109I	RGGNCCY	2 cut(s)	1378, 1650
EcoRI	GAATTC	1 cut(s)	617
EcoRII	CCWGG	5 cut(s)	576, 663, 908, 1206, 1606
EcoT14I	CCWWGG	2 cut(s)	1469, 1702
EcoT22I	ATGCAT	1 cut(s)	1204
EcoT38I	GRGCYC	1 cut(s)	39
EgeI	GGCGCC	1 cut(s)	127
EheI	GGCGCC	1 cut(s)	127
ErhI	CCWWGG	2 cut(s)	1469, 1702
Esp3I	CGTCTC	2 cut(s)	62, 97
FaeI	CATG	7 cut(s)	335, 559, 961, 975, 1282, 1444, 1638
FalI	AAGNNNNNCTT	2 cut(s)	887, 919
FaqI	GGGAC	4 cut(s)	310, 862, 949, 1057
FatI	CATG	7 cut(s)	331, 555, 957, 971, 1278, 1440, 1634
FauI	CCCGC	2 cut(s)	74, 199
FbaI	TGATCA	1 cut(s)	954
FblI	GTMKAC	2 cut(s)	30, 1465
Fnu4HI	GCNGC	6 cut(s)	235, 800, 803, 1131, 1293, 1674
FokI	GGATG	7 cut(s)	131, 494, 638, 857, 1052, 1685, 1732
FriOI	GRGCYC	1 cut(s)	39
Fsp4HI	GCNGC	6 cut(s)	235, 800, 803, 1131, 1293, 1674
GlaI	GCGC	6 cut(s)	74, 127, 312, 412, 550, 825
GluI	GCNGC	6 cut(s)	235, 800, 803, 1131, 1293, 1674
GsaI	CCCAGC	1 cut(s)	778
GsuI	CTGGAG	1 cut(s)	1146
HaeII	RGCGCY	5 cut(s)	76, 129, 314, 414, 552
HaeIII	GGCC	4 cut(s)	113, 350, 668, 1651
HapII	CCGG	7 cut(s)	135, 305, 495, 713, 721, 770, 785
HgaI	GACGC	1 cut(s)	226
HhaI	GCGC	6 cut(s)	75, 128, 313, 413, 551, 826
Hin1I	GRCGYC	2 cut(s)	126, 237
Hin1II	CATG	7 cut(s)	335, 559, 961, 975, 1282, 1444, 1638
Hin6I	GCGC	6 cut(s)	73, 126, 311, 411, 549, 824
HinP1I	GCGC	6 cut(s)	73, 126, 311, 411, 549, 824
HincII	GTYRAC	2 cut(s)	31, 204
HindII	GTYRAC	2 cut(s)	31, 204
HindIII	AAGCTT	1 cut(s)	455
HinfI	GANTC	8 cut(s)	205, 464, 581, 1502, 1514, 1549, 1691, 1742
HpaII	CCGG	7 cut(s)	135, 305, 495, 713, 721, 770, 785
HphI	GGTGA	4 cut(s)	79, 124, 232, 710
Hpy166II	GTNNAC	8 cut(s)	31, 204, 718, 1416, 1466, 1539, 1604, 1683
Hpy188I	TCNGA	6 cut(s)	389, 514, 1157, 1168, 1410, 1725
Hpy188III	TCNNGA	7 cut(s)	265, 587, 614, 872, 1247, 1306, 1522
Hpy8I	GTNNAC	8 cut(s)	31, 204, 718, 1416, 1466, 1539, 1604, 1683
Hpy99I	CGWCG	2 cut(s)	35, 178
HpyAV	CCTTC	5 cut(s)	61, 381, 835, 1369, 1705
HpyCH4III	ACNGT	7 cut(s)	91, 142, 433, 1253, 1313, 1492, 1687
HpyCH4IV	ACGT	4 cut(s)	539, 828, 938, 1731
HpyF3I	CTNAG	4 cut(s)	198, 1365, 1498, 1722
HpySE526I	ACGT	4 cut(s)	539, 828, 938, 1731
Hsp92I	GRCGYC	2 cut(s)	126, 237
Hsp92II	CATG	7 cut(s)	335, 559, 961, 975, 1282, 1444, 1638
HspAI	GCGC	6 cut(s)	73, 126, 311, 411, 549, 824
KasI	GGCGCC	1 cut(s)	125
KpnI	GGTACC	1 cut(s)	1269
Ksp22I	TGATCA	1 cut(s)	954
Kzo9I	GATC	8 cut(s)	384, 522, 543, 749, 954, 1157, 1561, 1610
LguI	GCTCTTC	1 cut(s)	36
LmnI	GCTCC	4 cut(s)	167, 808, 1127, 1453
Lsp1109I	GCAGC	5 cut(s)	786, 789, 1117, 1304, 1685
LweI	GCATC	5 cut(s)	279, 433, 616, 1178, 1189
MabI	ACCWGGT	1 cut(s)	908
MaeII	ACGT	4 cut(s)	539, 828, 938, 1731
MaeIII	GTNAC	8 cut(s)	85, 130, 238, 1247, 1253, 1313, 1630, 1643
MalI	GATC	8 cut(s)	386, 524, 545, 751, 956, 1159, 1563, 1612
MboI	GATC	8 cut(s)	384, 522, 543, 749, 954, 1157, 1561, 1610
MflI	RGATCY	1 cut(s)	749
MhlI	GDGCHC	4 cut(s)	39, 894, 971, 1418
MlsI	TGGCCA	1 cut(s)	668
MluCI	AATT	7 cut(s)	598, 617, 681, 700, 1238, 1404, 1735
MluNI	TGGCCA	1 cut(s)	668
Mly113I	GGCGCC	1 cut(s)	126
MlyI	GAGTC	3 cut(s)	199, 575, 1700
MmeI	TCCRAC	3 cut(s)	315, 1191, 1475
Mox20I	TGGCCA	1 cut(s)	668
Mph1103I	ATGCAT	1 cut(s)	1204
MroXI	GAANNNNTTC	1 cut(s)	1548
MscI	TGGCCA	1 cut(s)	668
MseI	TTAA	7 cut(s)	12, 221, 342, 417, 459, 861, 1116
MslI	CAYNNNNRTG	2 cut(s)	1534, 1750
Msp20I	TGGCCA	1 cut(s)	668
MspA1I	CMGCKG	1 cut(s)	1295
MspI	CCGG	7 cut(s)	135, 305, 495, 713, 721, 770, 785
Mva1269I	GAATGC	3 cut(s)	889, 1151, 1202
MvaI	CCWGG	5 cut(s)	578, 665, 910, 1208, 1608
MvnI	CGCG	1 cut(s)	824
NarI	GGCGCC	1 cut(s)	126
NciI	CCSGG	5 cut(s)	305, 770, 771, 785, 786
NdeII	GATC	8 cut(s)	384, 522, 543, 749, 954, 1157, 1561, 1610
NlaIII	CATG	7 cut(s)	335, 559, 961, 975, 1282, 1444, 1638
NlaIV	GGNNCC	7 cut(s)	127, 139, 189, 1165, 1267, 1380, 1449
NmeAIII	GCCGAG	1 cut(s)	1350
NmuCI	GTSAC	5 cut(s)	85, 130, 238, 1247, 1253
NsiI	ATGCAT	1 cut(s)	1204
NspI	RCATGY	1 cut(s)	335
PaeR7I	CTCGAG	1 cut(s)	265
PceI	AGGCCT	1 cut(s)	350
PciSI	GCTCTTC	1 cut(s)	36
PcsI	WCGNNNNNNNCGW	5 cut(s)	27, 36, 176, 263, 342
PctI	GAATGC	3 cut(s)	889, 1151, 1202
PdmI	GAANNNNTTC	1 cut(s)	1548
PfeI	GAWTC	5 cut(s)	464, 1502, 1514, 1549, 1742
Pfl23II	CGTACG	1 cut(s)	939
PflMI	CCANNNNNTGG	1 cut(s)	1147
PfoI	TCCNGGA	1 cut(s)	1206
PkrI	GCNGC	6 cut(s)	236, 801, 804, 1132, 1294, 1675
PleI	GAGTC	3 cut(s)	199, 575, 1699
PluTI	GGCGCC	1 cut(s)	129
PmaCI	CACGTG	1 cut(s)	829
PmlI	CACGTG	1 cut(s)	829
PpsI	GAGTC	3 cut(s)	199, 575, 1699
Ppu21I	YACGTR	1 cut(s)	829
PpuMI	RGGWCCY	1 cut(s)	1378
Psp124BI	GAGCTC	1 cut(s)	39
Psp5II	RGGWCCY	1 cut(s)	1378
Psp6I	CCWGG	5 cut(s)	576, 663, 908, 1206, 1606
PspCI	CACGTG	1 cut(s)	829
PspFI	CCCAGC	1 cut(s)	774
PspGI	CCWGG	5 cut(s)	576, 663, 908, 1206, 1606
PspLI	CGTACG	1 cut(s)	939
PspN4I	GGNNCC	7 cut(s)	127, 139, 189, 1165, 1267, 1380, 1449
PspPI	GGNCC	3 cut(s)	1378, 1604, 1650
PspPPI	RGGWCCY	1 cut(s)	1378
PstI	CTGCAG	1 cut(s)	807
PstNI	CAGNNNCTG	2 cut(s)	817, 1667
PsuI	RGATCY	1 cut(s)	749
PvuII	CAGCTG	1 cut(s)	1295
RsaI	GTAC	5 cut(s)	248, 737, 759, 941, 1267
RsaNI	GTAC	5 cut(s)	247, 736, 758, 940, 1266
RseI	CAYNNNNRTG	2 cut(s)	1534, 1750
SacI	GAGCTC	1 cut(s)	39
SalI	GTCGAC	1 cut(s)	29
SapI	GCTCTTC	1 cut(s)	36
SaqAI	TTAA	7 cut(s)	12, 221, 342, 417, 459, 861, 1116
SatI	GCNGC	6 cut(s)	235, 800, 803, 1131, 1293, 1674
Sau3AI	GATC	8 cut(s)	384, 522, 543, 749, 954, 1157, 1561, 1610
Sau96I	GGNCC	3 cut(s)	1378, 1604, 1650
SchI	GAGTC	3 cut(s)	199, 575, 1700
SduI	GDGCHC	4 cut(s)	39, 894, 971, 1418
SexAI	ACCWGGT	1 cut(s)	908
SfaNI	GCATC	5 cut(s)	279, 433, 616, 1178, 1189
SfcI	CTRYAG	3 cut(s)	803, 1074, 1183
SfoI	GGCGCC	1 cut(s)	127
Sfr274I	CTCGAG	1 cut(s)	265
SinI	GGWCC	2 cut(s)	1378, 1604
SlaI	CTCGAG	1 cut(s)	265
SmaI	CCCGGG	2 cut(s)	771, 786
SmiMI	CAYNNNNRTG	2 cut(s)	1534, 1750
SmlI	CTYRAG	1 cut(s)	265
SmoI	CTYRAG	1 cut(s)	265
Sse9I	AATT	7 cut(s)	598, 617, 681, 700, 1238, 1404, 1735
SseBI	AGGCCT	1 cut(s)	350
SsiI	CCGC	7 cut(s)	67, 154, 192, 213, 234, 822, 1145
SspDI	GGCGCC	1 cut(s)	125
SstI	GAGCTC	1 cut(s)	39
StuI	AGGCCT	1 cut(s)	350
StyI	CCWWGG	2 cut(s)	1469, 1702
TaaI	ACNGT	7 cut(s)	91, 142, 433, 1253, 1313, 1492, 1687
TaiI	ACGT	4 cut(s)	542, 831, 941, 1734
TasI	AATT	7 cut(s)	598, 617, 681, 700, 1238, 1404, 1735
TauI	GCSGC	1 cut(s)	237
TfiI	GAWTC	5 cut(s)	464, 1502, 1514, 1549, 1742
Tru1I	TTAA	7 cut(s)	12, 221, 342, 417, 459, 861, 1116
Tru9I	TTAA	7 cut(s)	12, 221, 342, 417, 459, 861, 1116
TscAI	CASTG	4 cut(s)	1126, 1267, 1366, 1399
TseFI	GTSAC	5 cut(s)	85, 130, 238, 1247, 1253
TseI	GCWGC	5 cut(s)	799, 802, 1130, 1292, 1673
Tsp45I	GTSAC	5 cut(s)	85, 130, 238, 1247, 1253
TspDTI	ATGAA	6 cut(s)	1081, 1102, 1267, 1687, 1725, 1734
TspGWI	ACGGA	4 cut(s)	44, 85, 200, 753
TspMI	CCCGGG	2 cut(s)	769, 784
TspRI	CASTG	4 cut(s)	1126, 1267, 1366, 1399
Van91I	CCANNNNNTGG	1 cut(s)	1147
VneI	GTGCAC	1 cut(s)	1414
VpaK11BI	GGWCC	2 cut(s)	1378, 1604
XapI	RAATTY	3 cut(s)	617, 681, 1735
XceI	RCATGY	1 cut(s)	335
XcmI	CCANNNNNNNNNTGG	1 cut(s)	661
XhoI	CTCGAG	1 cut(s)	265
XmaI	CCCGGG	2 cut(s)	769, 784
XmiI	GTMKAC	2 cut(s)	30, 1465
XmnI	GAANNNNTTC	1 cut(s)	1548
Zsp2I	ATGCAT	1 cut(s)	1204

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.