Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0015780

Physical Location & Seq

Genomic Coordinates Reverse (-)

14600611 .. 14604442

Gene Structure 3832 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1154400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1761 bp

ATGGACTCCATTAAGTCCTTCAAGGGCTACGGCAAAGTGGACGTGCTCGAAGAGCAGTCGTTCAAGCGCAAAAACCGGAAGCGTCTCATCATCCTCTCAGTCTCCACGGTTGTTCTGCTGGCGGTGATCATCGGCGCTGTCGCGGGAGTATTGATACACAAGCGGAACAACACCTCGTCTTCTACACCCAACTCGACCCCCGGCACTGAGTTGACCCCGGCGGCGTCGCTGAAGGCTGTTTGTGACGTGACCCAGTACCCGACTTCGTGTTTCTCTAGTATATCGGCACTTGAGACCTCGAACACCACCGACCCAGAAGTGATTTTCAAGCTCTCTCTGCAGGTCGCCATTCACGCCGCCGCGAAGCTCCCCGGTCTGCCGGCGAAAATCGGCCGGAACATCGTCAACGACACCCGCCTGGAGAAAGTTCTGGCCGTCTGCGAGGGCTTGTTTGAGGACGTGGTCGACCGGCTCAACGACTCGCTGACCTCCATGGATGTGAATCAGGGTGAGAAGCTTCTGTCCGCCGCCAAAATCAACGACATCAAGACGTGGCTGAGCACCACATTGACAGATCAGGAGACTTGTTTGGACTCTCTGATGGATCTCAATTCGACCCTCGTTCAGGAATTCAAGGCGGCGATGCAGAATTCAACCGAATTTTCGAGCAATAGTTTGGCGATTGTGGCGAAAATTTTGAGTCTGCTGACGAATTTGAACGTTCCGGTTCACCGGAGGCTGCTCGGGGTCGGAGGATCCGAGATGGGTTTTCCCAGTTGGGTTAGTCCGGGGGATAGGAGGCTGCTGCAGGACCGCAATGCGACGGTGGCGCAGATAGTGGTGGCGAAGGACGGGACCGGAGACTTTAAGACGATTCAGGAGGCGGTGGATGCGGTGCCGAAGAAGAGCGAGAACAGGATTGTGATTCATGTAAAGCAAGGGGTTTATTATGAGAATGTGTTGATGGATAAGAGCAAGTGGAATGTGATGATGTTCGGAGATGGGAAGACGAAGACGGTTGTTTCCGGCAACTTGAGCTCTGCGGGCGGCACCCCCACCTTCTCCACCGCCACATTTGCTGTCACGGGAAAGGGCTTCATTGTGAAAGATATGAGATTCATCAACACTGCAGGAGCAATAAAGCACCAGGCAGTGGCACTCCGCTCTGGTTCAGACCTCTCTGTGTACTACAGATGCTCGTTTGATGGCTTCCAGGATACCCTCTATGCGCATTCCAACCGACAATTCTATCGGGAATGTGACATCACCGGCACCATAGACTTCATTTTCGGCAATGCAGCTGTAGTTTTCCAGGACTGCAACATCCAGCCCAGGCAACCTATGGAAAACCAATTCAACACCATCACAGCTCAAGGCAAAAAGGACCCGAATCAGAACACCGGCATTTCAATCCAAAATTGCAGATTCTATGCGCTAGACAGTAATCTCACAGCTCAAACATATCTGGGCAGGCCTTGGAAGGATTACTCCACCACAGTCATCATGCAGTCTGAGATTGGATCATTCTTGAACCCATCGGGCTGGAAAGAATGGGTTACAAATGTTGACCCACCCAACACCATATTCTATGCAGAGTACCAAAACAGTGGCCCTGGATCAGGTGTAGATCAAAGGGTTAAGTGGGCGGGTTACAGGCCTACTCTTACCGATAACGAGGCGGTCAAGTTCACGGTAACGTCATTGATCCAAGGCGATGAGTGGTTACCGTCGACTGATGTAACATTTCAGTCTTCGTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0008150 GO:0009506 GO:0012505 GO:0016020 GO:0016787 GO:0016788 GO:0030054 GO:0030234 GO:0030599 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0043086 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044092 GO:0044422 GO:0044424 GO:0044431 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046910 GO:0050790 GO:0051722 GO:0051723 GO:0052689 GO:0055044 GO:0065007 GO:0065009 GO:0071944 GO:0097708 GO:0098588 GO:0098772 GO:0098791 GO:0098805 GO:0140096

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

587

Amino Acids

64.24

Weight (kDa)

7.0

Isoelectric Point (pI)

29.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	76 - 228	1.1e-27	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	279 - 572	1.5e-139	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015780)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G53840 AT3G14300
fragaria_vesca	FvH4_6g35820
malus_domestica	MD09G1172900.v1.1 MD17G1154400.v1.1
rosa_chinensis	RchiOBHm_Chr2g0147861
rosa_laevigata	RLG00000020286
rosa_multiflora	Rmu_sc0000308.1_g000054
rosa_roxburghii	Rroxscaffold_2G00099260
rosa_rugosa	Rorug02G0402500
rosa_samantha	Rh2AG459400 Rh2BG472800 Rh2CG446800 Rh2DG481700
rosa_wichuraiana	Rw2G037580

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1230
Acc36I	ACCTGC	1 cut(s)	331
AccB1I	GGYRCC	3 cut(s)	895, 1049, 1271
AccB7I	CCANNNNNTGG	1 cut(s)	1153
AccBSI	CCGCTC	1 cut(s)	1164
AccI	GTMKAC	2 cut(s)	465, 1730
AccII	CGCG	2 cut(s)	143, 362
AclI	AACGTT	1 cut(s)	720
AclWI	GGATC	6 cut(s)	612, 750, 763, 1528, 1624, 1699
AcoI	YGGCCR	2 cut(s)	391, 432
AcsI	RAATTY	5 cut(s)	629, 649, 659, 693, 712
AcuI	CTGAAG	1 cut(s)	251
AcyI	GRCGYC	1 cut(s)	224
AfaI	GTAC	3 cut(s)	257, 1187, 1598
AfiI	CCNNNNNNNGG	3 cut(s)	625, 1153, 1619
AgsI	TTSAA	9 cut(s)	22, 64, 328, 634, 654, 718, 1357, 1410, 1531
AjiI	CACGTC	4 cut(s)	43, 247, 460, 552
AjnI	CCWGG	6 cut(s)	417, 1146, 1212, 1311, 1331, 1612
AjuI	GAANNNNNNNTTGG	4 cut(s)	1229, 1261, 1568, 1600
AluBI	AGCT	7 cut(s)	331, 367, 517, 1038, 1301, 1370, 1454
AluI	AGCT	7 cut(s)	331, 367, 517, 1038, 1301, 1370, 1454
Alw21I	GWGCWC	3 cut(s)	48, 563, 1040
Alw26I	GTCTC	5 cut(s)	89, 106, 287, 575, 855
AlwI	GGATC	6 cut(s)	612, 750, 763, 1528, 1624, 1699
Ama87I	CYCGRG	1 cut(s)	743
AoxI	GGCC	5 cut(s)	391, 432, 1472, 1609, 1655
ApeKI	GCWGC	4 cut(s)	739, 802, 805, 1298
ApoI	RAATTY	5 cut(s)	629, 649, 659, 693, 712
ArsI	GACNNNNNNTTYG	2 cut(s)	1271, 1303
AspLEI	GCGC	5 cut(s)	69, 137, 832, 1231, 1435
AspS9I	GGNCC	4 cut(s)	811, 855, 1384, 1610
AsuC2I	CCSGG	4 cut(s)	201, 218, 372, 789
AsuHPI	GGTGA	4 cut(s)	136, 521, 722, 1258
AvaI	CYCGRG	1 cut(s)	743
AvaII	GGWCC	3 cut(s)	811, 855, 1384
BamHI	GGATCC	1 cut(s)	755
BanI	GGYRCC	3 cut(s)	895, 1049, 1271
BanII	GRGCYC	1 cut(s)	1040
BbsI	GAAGAC	4 cut(s)	171, 1013, 1019, 1743
Bbv12I	GWGCWC	3 cut(s)	48, 563, 1040
BbvI	GCAGC	4 cut(s)	726, 789, 792, 1310
BccI	CCATC	7 cut(s)	595, 757, 958, 995, 1199, 1370, 1543
BceAI	ACGGC	2 cut(s)	46, 419
BciT130I	CCWGG	6 cut(s)	419, 1148, 1214, 1313, 1333, 1614
BciVI	GTATCC	1 cut(s)	1210
BclI	TGATCA	1 cut(s)	126
BcnI	CCSGG	4 cut(s)	201, 218, 372, 789
BcoDI	GTCTC	5 cut(s)	89, 106, 287, 575, 855
BfaI	CTAG	2 cut(s)	276, 1436
BfmI	CTRYAG	5 cut(s)	338, 806, 1128, 1189, 1302
BfoI	RGCGCY	1 cut(s)	138
BfuAI	ACCTGC	1 cut(s)	331
BfuI	GTATCC	1 cut(s)	1210
BlpI	GCTNAGC	1 cut(s)	557
Bme18I	GGWCC	3 cut(s)	811, 855, 1384
BmeT110I	CYCGRG	1 cut(s)	743
BmgBI	CACGTC	4 cut(s)	43, 247, 460, 552
BmgT120I	GGNCC	4 cut(s)	811, 855, 1384, 1610
BmiI	GGNNCC	6 cut(s)	757, 856, 897, 1051, 1273, 1386
BmrI	ACTGGG	2 cut(s)	247, 768
BmsI	GCATC	3 cut(s)	633, 880, 1184
BmuI	ACTGGG	2 cut(s)	247, 768
BpiI	GAAGAC	4 cut(s)	171, 1013, 1019, 1743
BpmI	CTGGAG	1 cut(s)	440
Bpu1102I	GCTNAGC	1 cut(s)	557
BpuEI	CTTGAG	3 cut(s)	311, 1054, 1356
BpuMI	CCSGG	4 cut(s)	201, 218, 372, 789
BsaBI	GATNNNNATC	1 cut(s)	501
BsaHI	GRCGYC	1 cut(s)	224
BsaI	GGTCTC	1 cut(s)	287
BsaWI	WCCGGW	4 cut(s)	75, 724, 732, 857
Bsc4I	CCNNNNNNNGG	3 cut(s)	625, 1153, 1619
Bse118I	RCCGGY	4 cut(s)	379, 468, 1268, 1400
Bse1I	ACTGG	2 cut(s)	253, 774
Bse3DI	GCAATG	2 cut(s)	823, 1300
Bse8I	GATNNNNATC	1 cut(s)	501
BseBI	CCWGG	6 cut(s)	419, 1148, 1214, 1313, 1333, 1614
BseGI	GGATG	4 cut(s)	90, 502, 895, 1323
BseJI	GATNNNNATC	1 cut(s)	501
BseLI	CCNNNNNNNGG	3 cut(s)	625, 1153, 1619
BseMI	GCAATG	2 cut(s)	823, 1300
BseMII	CTCAG	4 cut(s)	111, 198, 548, 1503
BseNI	ACTGG	2 cut(s)	253, 774
BseX3I	CGGCCG	1 cut(s)	391
BseXI	GCAGC	4 cut(s)	726, 789, 792, 1310
Bsh1236I	CGCG	2 cut(s)	143, 362
Bsh1285I	CGRYCG	2 cut(s)	394, 469
BshFI	GGCC	5 cut(s)	393, 434, 1474, 1611, 1657
BshNI	GGYRCC	3 cut(s)	895, 1049, 1271
BsiEI	CGRYCG	2 cut(s)	394, 469
BsiHKAI	GWGCWC	3 cut(s)	48, 563, 1040
BsiHKCI	CYCGRG	1 cut(s)	743
BslFI	GGGAC	1 cut(s)	868
BslI	CCNNNNNNNGG	3 cut(s)	625, 1153, 1619
BsmAI	GTCTC	5 cut(s)	89, 106, 287, 575, 855
BsmBI	CGTCTC	1 cut(s)	89
BsmFI	GGGAC	1 cut(s)	868
BsmI	GAATGC	1 cut(s)	1231
BsnI	GGCC	5 cut(s)	393, 434, 1474, 1611, 1657
Bso31I	GGTCTC	1 cut(s)	287
BsoBI	CYCGRG	1 cut(s)	743
Bsp1286I	GDGCHC	3 cut(s)	48, 563, 1040
Bsp143I	GATC	8 cut(s)	126, 574, 604, 755, 1520, 1616, 1627, 1704
Bsp1720I	GCTNAGC	1 cut(s)	557
Bsp19I	CCATGG	1 cut(s)	492
BspANI	GGCC	5 cut(s)	393, 434, 1474, 1611, 1657
BspCNI	CTCAG	4 cut(s)	110, 199, 549, 1504
BspFNI	CGCG	2 cut(s)	143, 362
BspLI	GGNNCC	6 cut(s)	757, 856, 897, 1051, 1273, 1386
BspMAI	CTGCAG	3 cut(s)	342, 810, 1132
BspMI	ACCTGC	1 cut(s)	331
BspPI	GGATC	6 cut(s)	612, 750, 763, 1528, 1624, 1699
BspQI	GCTCTTC	2 cut(s)	45, 899
BspT107I	GGYRCC	3 cut(s)	895, 1049, 1271
BspTNI	GGTCTC	1 cut(s)	287
BsrBI	CCGCTC	1 cut(s)	1164
BsrDI	GCAATG	2 cut(s)	823, 1300
BsrFI	RCCGGY	4 cut(s)	379, 468, 1268, 1400
BsrI	ACTGG	2 cut(s)	253, 774
BssAI	RCCGGY	4 cut(s)	379, 468, 1268, 1400
BssMI	GATC	8 cut(s)	126, 574, 604, 755, 1520, 1616, 1627, 1704
BssNI	GRCGYC	1 cut(s)	224
BssT1I	CCWWGG	3 cut(s)	492, 1475, 1708
Bst2UI	CCWGG	6 cut(s)	419, 1148, 1214, 1313, 1333, 1614
Bst4CI	ACNGT	8 cut(s)	109, 826, 1018, 1442, 1498, 1607, 1693, 1728
Bst6I	CTCTTC	2 cut(s)	45, 899
BstACI	GRCGYC	1 cut(s)	224
BstC8I	GCNNGC	4 cut(s)	120, 381, 1045, 1472
BstDEI	CTNAG	4 cut(s)	97, 207, 557, 1512
BstDSI	CCRYGG	2 cut(s)	105, 492
BstEII	GGTNACC	1 cut(s)	1722
BstENI	CCTNNNNNAGG	2 cut(s)	623, 1617
BstF5I	GGATG	4 cut(s)	90, 502, 895, 1323
BstFNI	CGCG	2 cut(s)	143, 362
BstH2I	RGCGCY	1 cut(s)	138
BstHHI	GCGC	5 cut(s)	69, 137, 832, 1231, 1435
BstKTI	GATC	8 cut(s)	129, 577, 607, 758, 1523, 1619, 1630, 1707
BstMAI	GTCTC	5 cut(s)	89, 106, 287, 575, 855
BstMBI	GATC	8 cut(s)	126, 574, 604, 755, 1520, 1616, 1627, 1704
BstMCI	CGRYCG	2 cut(s)	394, 469
BstMWI	GCNNNNNNNGC	9 cut(s)	52, 337, 353, 686, 827, 890, 1035, 1044, 1076
BstNI	CCWGG	6 cut(s)	419, 1148, 1214, 1313, 1333, 1614
BstPI	GGTNACC	1 cut(s)	1722
BstSFI	CTRYAG	5 cut(s)	338, 806, 1128, 1189, 1302
BstUI	CGCG	2 cut(s)	143, 362
BstV1I	GCAGC	4 cut(s)	726, 789, 792, 1310
BstV2I	GAAGAC	4 cut(s)	171, 1013, 1019, 1743
BstX2I	RGATCY	2 cut(s)	604, 755
BstXI	CCANNNNNNTGG	2 cut(s)	1542, 1607
BstYI	RGATCY	2 cut(s)	604, 755
BstZI	CGGCCG	1 cut(s)	391
BsuI	GTATCC	1 cut(s)	1210
BsuRI	GGCC	5 cut(s)	393, 434, 1474, 1611, 1657
BtgI	CCRYGG	2 cut(s)	105, 492
BtgZI	GCGATG	2 cut(s)	656, 1728
BtrI	CACGTC	4 cut(s)	43, 247, 460, 552
BtsCI	GGATG	4 cut(s)	90, 502, 895, 1323
BtsI	GCAGTG	2 cut(s)	1125, 1158
BtsIMutI	CAGTG	4 cut(s)	204, 1125, 1158, 1612
BveI	ACCTGC	1 cut(s)	331
Cac8I	GCNNGC	4 cut(s)	120, 381, 1045, 1472
CfoI	GCGC	5 cut(s)	69, 137, 832, 1231, 1435
Cfr10I	RCCGGY	4 cut(s)	379, 468, 1268, 1400
Cfr13I	GGNCC	4 cut(s)	811, 855, 1384, 1610
CseI	GACGC	2 cut(s)	71, 213
Csp6I	GTAC	3 cut(s)	256, 1186, 1597
CviAII	CATG	3 cut(s)	493, 929, 1504
CviQI	GTAC	3 cut(s)	256, 1186, 1597
DdeI	CTNAG	4 cut(s)	97, 207, 557, 1512
DpnI	GATC	8 cut(s)	128, 576, 606, 757, 1522, 1618, 1629, 1706
DpnII	GATC	8 cut(s)	126, 574, 604, 755, 1520, 1616, 1627, 1704
EaeI	YGGCCR	2 cut(s)	391, 432
EagI	CGGCCG	1 cut(s)	391
Eam1104I	CTCTTC	2 cut(s)	45, 899
EarI	CTCTTC	2 cut(s)	45, 899
EciI	GGCGGA	1 cut(s)	514
Ecl136II	GAGCTC	1 cut(s)	1038
EclXI	CGGCCG	1 cut(s)	391
Eco130I	CCWWGG	3 cut(s)	492, 1475, 1708
Eco147I	AGGCCT	2 cut(s)	1474, 1657
Eco24I	GRGCYC	1 cut(s)	1040
Eco31I	GGTCTC	1 cut(s)	287
Eco47I	GGWCC	3 cut(s)	811, 855, 1384
Eco52I	CGGCCG	1 cut(s)	391
Eco53kI	GAGCTC	1 cut(s)	1038
Eco57I	CTGAAG	1 cut(s)	251
Eco88I	CYCGRG	1 cut(s)	743
Eco91I	GGTNACC	1 cut(s)	1722
EcoICRI	GAGCTC	1 cut(s)	1038
EcoNI	CCTNNNNNAGG	2 cut(s)	623, 1617
EcoO109I	RGGNCCY	1 cut(s)	1384
EcoO65I	GGTNACC	1 cut(s)	1722
EcoRI	GAATTC	2 cut(s)	629, 649
EcoRII	CCWGG	6 cut(s)	417, 1146, 1212, 1311, 1331, 1612
EcoT14I	CCWWGG	3 cut(s)	492, 1475, 1708
EcoT38I	GRGCYC	1 cut(s)	1040
ErhI	CCWWGG	3 cut(s)	492, 1475, 1708
Esp3I	CGTCTC	1 cut(s)	89
FaeI	CATG	3 cut(s)	496, 932, 1507
FaqI	GGGAC	1 cut(s)	868
FatI	CATG	3 cut(s)	492, 928, 1503
FauI	CCCGC	4 cut(s)	136, 422, 1036, 1639
FbaI	TGATCA	1 cut(s)	126
FblI	GTMKAC	2 cut(s)	465, 1730
FokI	GGATG	4 cut(s)	77, 509, 902, 1310
FriOI	GRGCYC	1 cut(s)	1040
FspAI	RTGCGCAY	1 cut(s)	1230
FspBI	CTAG	2 cut(s)	276, 1436
FspI	TGCGCA	1 cut(s)	1230
GlaI	GCGC	5 cut(s)	68, 136, 831, 1230, 1434
GsuI	CTGGAG	1 cut(s)	440
HaeII	RGCGCY	1 cut(s)	138
HaeIII	GGCC	5 cut(s)	393, 434, 1474, 1611, 1657
HgaI	GACGC	2 cut(s)	71, 213
HhaI	GCGC	5 cut(s)	69, 137, 832, 1231, 1435
Hin1I	GRCGYC	1 cut(s)	224
Hin1II	CATG	3 cut(s)	496, 932, 1507
Hin6I	GCGC	5 cut(s)	67, 135, 830, 1229, 1433
HinP1I	GCGC	5 cut(s)	67, 135, 830, 1229, 1433
HincII	GTYRAC	5 cut(s)	213, 406, 466, 1567, 1731
HindII	GTYRAC	5 cut(s)	213, 406, 466, 1567, 1731
HindIII	AAGCTT	1 cut(s)	515
HphI	GGTGA	4 cut(s)	136, 521, 722, 1258
Hpy166II	GTNNAC	9 cut(s)	40, 213, 406, 466, 730, 1186, 1567, 1689, 1731
Hpy188I	TCNGA	7 cut(s)	600, 752, 760, 998, 1174, 1395, 1513
Hpy188III	TCNNGA	6 cut(s)	547, 578, 626, 878, 1253, 1528
Hpy8I	GTNNAC	9 cut(s)	40, 213, 406, 466, 730, 1186, 1567, 1689, 1731
Hpy99I	CGWCG	3 cut(s)	229, 826, 1732
HpyAV	CCTTC	5 cut(s)	28, 226, 841, 1069, 1474
HpyCH4III	ACNGT	8 cut(s)	109, 826, 1018, 1442, 1498, 1607, 1693, 1728
HpyCH4IV	ACGT	6 cut(s)	42, 246, 459, 551, 720, 1697
HpyCH4V	TGCA	9 cut(s)	340, 646, 808, 1130, 1298, 1320, 1422, 1507, 1592
HpyF10VI	GCNNNNNNNGC	9 cut(s)	52, 337, 353, 686, 827, 890, 1035, 1044, 1076
HpyF3I	CTNAG	4 cut(s)	97, 207, 557, 1512
HpySE526I	ACGT	6 cut(s)	42, 246, 459, 551, 720, 1697
Hsp92I	GRCGYC	1 cut(s)	224
Hsp92II	CATG	3 cut(s)	496, 932, 1507
HspAI	GCGC	5 cut(s)	67, 135, 830, 1229, 1433
KroI	GCCGGC	1 cut(s)	379
KroNI	GCCGGC	1 cut(s)	381
Ksp22I	TGATCA	1 cut(s)	126
Kzo9I	GATC	8 cut(s)	126, 574, 604, 755, 1520, 1616, 1627, 1704
LguI	GCTCTTC	2 cut(s)	45, 899
LmnI	GCTCC	2 cut(s)	372, 1133
Lsp1109I	GCAGC	4 cut(s)	726, 789, 792, 1310
LweI	GCATC	3 cut(s)	633, 880, 1184
MaeI	CTAG	2 cut(s)	276, 1436
MaeII	ACGT	6 cut(s)	42, 246, 459, 551, 720, 1697
MaeIII	GTNAC	9 cut(s)	242, 247, 1081, 1259, 1555, 1649, 1693, 1722, 1738
MalI	GATC	8 cut(s)	128, 576, 606, 757, 1522, 1618, 1629, 1706
MbiI	CCGCTC	1 cut(s)	1164
MboI	GATC	8 cut(s)	126, 574, 604, 755, 1520, 1616, 1627, 1704
MboII	GAAGA	7 cut(s)	62, 171, 913, 916, 1018, 1024, 1743
MflI	RGATCY	2 cut(s)	604, 755
MhlI	GDGCHC	3 cut(s)	48, 563, 1040
MluCI	AATT	9 cut(s)	610, 629, 649, 659, 693, 712, 1244, 1352, 1417
MlyI	GAGTC	3 cut(s)	473, 587, 709
MmeI	TCCRAC	2 cut(s)	730, 1260
MroNI	GCCGGC	1 cut(s)	379
MseI	TTAA	3 cut(s)	12, 867, 1638
MslI	CAYNNNNRTG	1 cut(s)	497
MspA1I	CMGCKG	1 cut(s)	1301
Mva1269I	GAATGC	1 cut(s)	1231
MvaI	CCWGG	6 cut(s)	419, 1148, 1214, 1313, 1333, 1614
MvnI	CGCG	2 cut(s)	143, 362
MwoI	GCNNNNNNNGC	9 cut(s)	52, 337, 353, 686, 827, 890, 1035, 1044, 1076
NaeI	GCCGGC	1 cut(s)	381
NciI	CCSGG	4 cut(s)	201, 218, 372, 789
NcoI	CCATGG	1 cut(s)	492
NdeII	GATC	8 cut(s)	126, 574, 604, 755, 1520, 1616, 1627, 1704
NgoMIV	GCCGGC	1 cut(s)	379
NlaIII	CATG	3 cut(s)	496, 932, 1507
NlaIV	GGNNCC	6 cut(s)	757, 856, 897, 1051, 1273, 1386
NmuCI	GTSAC	4 cut(s)	242, 247, 1081, 1259
NsbI	TGCGCA	1 cut(s)	1230
PceI	AGGCCT	2 cut(s)	1474, 1657
PciSI	GCTCTTC	2 cut(s)	45, 899
PcsI	WCGNNNNNNNCGW	1 cut(s)	756
PctI	GAATGC	1 cut(s)	1231
PdiI	GCCGGC	1 cut(s)	381
PfeI	GAWTC	6 cut(s)	502, 874, 925, 1116, 1390, 1425
PflFI	GACNNNGTC	1 cut(s)	461
PflMI	CCANNNNNTGG	1 cut(s)	1153
PfoI	TCCNGGA	2 cut(s)	1212, 1311
PleI	GAGTC	3 cut(s)	473, 587, 708
PpsI	GAGTC	3 cut(s)	473, 587, 708
PpuMI	RGGWCCY	1 cut(s)	1384
Psp124BI	GAGCTC	1 cut(s)	1040
Psp1406I	AACGTT	1 cut(s)	720
Psp5II	RGGWCCY	1 cut(s)	1384
Psp6I	CCWGG	6 cut(s)	417, 1146, 1212, 1311, 1331, 1612
PspEI	GGTNACC	1 cut(s)	1722
PspGI	CCWGG	6 cut(s)	417, 1146, 1212, 1311, 1331, 1612
PspN4I	GGNNCC	6 cut(s)	757, 856, 897, 1051, 1273, 1386
PspPI	GGNCC	4 cut(s)	811, 855, 1384, 1610
PspPPI	RGGWCCY	1 cut(s)	1384
PstI	CTGCAG	3 cut(s)	342, 810, 1132
PsuI	RGATCY	2 cut(s)	604, 755
PsyI	GACNNNGTC	1 cut(s)	461
PvuII	CAGCTG	1 cut(s)	1301
RsaI	GTAC	3 cut(s)	257, 1187, 1598
RsaNI	GTAC	3 cut(s)	256, 1186, 1597
RseI	CAYNNNNRTG	1 cut(s)	497
SacI	GAGCTC	1 cut(s)	1040
SalI	GTCGAC	2 cut(s)	464, 1729
SapI	GCTCTTC	2 cut(s)	45, 899
SaqAI	TTAA	3 cut(s)	12, 867, 1638
Sau3AI	GATC	8 cut(s)	126, 574, 604, 755, 1520, 1616, 1627, 1704
Sau96I	GGNCC	4 cut(s)	811, 855, 1384, 1610
SchI	GAGTC	3 cut(s)	473, 587, 709
SduI	GDGCHC	3 cut(s)	48, 563, 1040
SfaNI	GCATC	3 cut(s)	633, 880, 1184
SfcI	CTRYAG	5 cut(s)	338, 806, 1128, 1189, 1302
SinI	GGWCC	3 cut(s)	811, 855, 1384
SmiMI	CAYNNNNRTG	1 cut(s)	497
SmlI	CTYRAG	3 cut(s)	290, 1033, 1371
SmoI	CTYRAG	3 cut(s)	290, 1033, 1371
Sse9I	AATT	9 cut(s)	610, 629, 649, 659, 693, 712, 1244, 1352, 1417
SseBI	AGGCCT	2 cut(s)	1474, 1657
SspMI	CTAG	2 cut(s)	276, 1436
SstI	GAGCTC	1 cut(s)	1040
StuI	AGGCCT	2 cut(s)	1474, 1657
StyI	CCWWGG	3 cut(s)	492, 1475, 1708
TaaI	ACNGT	8 cut(s)	109, 826, 1018, 1442, 1498, 1607, 1693, 1728
TaiI	ACGT	6 cut(s)	45, 249, 462, 554, 723, 1700
TaqI	TCGA	7 cut(s)	48, 194, 299, 465, 614, 665, 1730
TasI	AATT	9 cut(s)	610, 629, 649, 659, 693, 712, 1244, 1352, 1417
TatI	WGTACW	1 cut(s)	1185
TauI	GCSGC	6 cut(s)	224, 359, 362, 530, 641, 1050
TfiI	GAWTC	6 cut(s)	502, 874, 925, 1116, 1390, 1425
Tru1I	TTAA	3 cut(s)	12, 867, 1638
Tru9I	TTAA	3 cut(s)	12, 867, 1638
TscAI	CASTG	4 cut(s)	211, 1132, 1158, 1612
TseFI	GTSAC	4 cut(s)	242, 247, 1081, 1259
TseI	GCWGC	4 cut(s)	739, 802, 805, 1298
Tsp45I	GTSAC	4 cut(s)	242, 247, 1081, 1259
TspDTI	ATGAA	4 cut(s)	917, 1087, 1108, 1273
TspRI	CASTG	4 cut(s)	211, 1132, 1158, 1612
Tth111I	GACNNNGTC	1 cut(s)	461
Van91I	CCANNNNNTGG	1 cut(s)	1153
VpaK11BI	GGWCC	3 cut(s)	811, 855, 1384
XagI	CCTNNNNNAGG	2 cut(s)	623, 1617
XapI	RAATTY	5 cut(s)	629, 649, 659, 693, 712
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1339
XmiI	GTMKAC	2 cut(s)	465, 1730
XspI	CTAG	2 cut(s)	276, 1436

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.