FvH4_6g37020

Eukaryotic family of unknown function (DUF1754)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012401
Physical Location & Seq
Reverse (-)
29068234 .. 29074878
6645 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g37020.t2

Sequence Viewer

Length: 1242 bp
ATGGTGGAGAGCAAGACTGTGCACTCACCATTGGTGACTTACGCTTCCATGCTTTCACTGCTTACACTCTGCCCTCCATTTGTGATTCTACTATGGTATACAATGGTACATGCTGATGGCTCTCTTTTACAAACTTGGGAGTACTTGAGACAAAATGGGCTGCAGGGGTTTATAGACATATGGCCAAGACCTACTGCTATTGCCTGGAAAATCATTGCATGTTATGGCGCTTTTGAGGCTGCACTTCAGCTGCTTTTACCTGGAAAAAGGGTCGAGGGACCAATATCTCCGGCTGGGAATCGGCCTGTGTACAAGGCAAATGGTATGGCAGCATACTTTGTGACATTGACTACATATATTGGCCTTTGGTGGTTTGGGATATTCAACCCTACAATTGTTTATGATCATTTGGGAGAAATATTTTCGGCCCTCATTTTTGGAAGTTTGGTGTTTTGTGTTCTCCTATACATAAAAGGTCACTTGGTGCCATCTTCCACAGATTCTGGCTCTTCTGGGAATGTTATAATTGATTTTTACTGGGGCATGGAACTGTATCCTCGAATTGGTGAACACTTTGATATCAAAGTTTTCACGAACTGTAGATTTGGAATGATGTCCTGGGCCGTTCTTGCAGTGACCTATTGCATAAAGCAGTATGAGTTGAATGGGAGAGTAGCTGATTCAATGCTCGTAAATACTATACTGATGCTGGTGTATGTCACAAAATTCTTCTGGTGGGAGGCTGGATACTGGAATACAATGGATATTGCACATGATCGAGCGGGATTTTATATTTGCTGGGGTTGTTTGGTGTGGGTTCCATCTATTTATACCTCACCTGGCATGTACTTGGTCAACCATCCTATAAATCTCGGCAGTCAGCTAGCACTTCTTATTTTGGTTGCGGGGATTCTCTGCATATACATCAACTATGATTGCGACAGGCAAAGACAAGAATTTCGCAGAACAAATGGCAAATGTTTAGTCTGGGGGAGAGCTCCATCAAAGATAGTTGCTTCATACACTACACAATCTGGGGAAACAAAGACAAGTCTTCTCTTAACCTCAGGATGGTGGGGTTTATCTCGCCATTTCCATTATGTGCCAGAAATATCAGCTGCCTTTTTCTGGACTGTTCCAGCTCTTTTCAATCATTTTCTACCGTACTTCTATGTGGTGTTTCTTACAATCCTTCTATTAGACCGAGCAAAAAGGGATGATGATCGATGTCGATCCAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00100 ko01100 ko01110 map00100 map01100 map01110
Pfam Domains
Protein Families
DUF1754 ERG4_ERG24

Protein Analysis

414

Amino Acids

47.15

Weight (kDa)

8.39

Isoelectric Point (pI)

36.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ERG4_ERG24 PF01222 41 - 434 2.8e-85 Ergosterol biosynthesis ERG4/ERG24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012401)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50430 AT1G50430
fragaria_vesca FvH4_6g37020 FvH4_6g37020 FvH4_6g37020
malus_domestica MD09G1164900.v1.1 MD17G1163300.v1.1
prunus_persica Prupe.3G022300_v2.0.a1 Prupe.3G022300_v2.0.a1
pyrus_communis pycom09g08210
rosa_chinensis RchiOBHm_Chr2g0149571
rosa_laevigata RLG00000020424
rosa_multiflora Rmu_sc0006867.1_g000021
rosa_roxburghii Rroxscaffold_2G00097980
rosa_rugosa Rorug02G0412400
rosa_samantha Rh2AG473000 Rh2BG484900 Rh2DG494100
rosa_wichuraiana Rw2G038550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 524
AccB1I GGYRCC 1 cut(s) 484
AccBSI CCGCTC 1 cut(s) 782
AccI GTMKAC 1 cut(s) 98
AciI CCGC 2 cut(s) 782, 905
AclWI GGATC 1 cut(s) 1227
AcoI YGGCCR 1 cut(s) 182
AcsI RAATTY 2 cut(s) 725, 956
AcuI CTGAAG 1 cut(s) 230
AdeI CACNNNGTG 1 cut(s) 484
AfaI GTAC 5 cut(s) 108, 143, 311, 848, 1166
AfiI CCNNNNNNNGG 3 cut(s) 563, 1071, 1128
AgsI TTSAA 4 cut(s) 385, 664, 684, 1150
AjnI CCWGG 4 cut(s) 203, 259, 617, 838
AluBI AGCT 6 cut(s) 250, 677, 883, 998, 1118, 1142
AluI AGCT 6 cut(s) 250, 677, 883, 998, 1118, 1142
Alw21I GWGCWC 2 cut(s) 24, 1000
Alw26I GTCTC 1 cut(s) 142
Alw44I GTGCAC 1 cut(s) 20
AlwI GGATC 1 cut(s) 1227
AlwNI CAGNNNCTG 1 cut(s) 503
AoxI GGCC 5 cut(s) 182, 302, 361, 426, 621
ApaLI GTGCAC 1 cut(s) 20
ApeKI GCWGC 5 cut(s) 160, 239, 250, 329, 1118
ApoI RAATTY 2 cut(s) 725, 956
ArsI GACNNNNNNTTYG 4 cut(s) 942, 969, 974, 1001
AspLEI GCGC 1 cut(s) 230
AspS9I GGNCC 3 cut(s) 278, 427, 621
AsuHPI GGTGA 4 cut(s) 18, 46, 578, 828
AsuNHI GCTAGC 1 cut(s) 883
AvaII GGWCC 1 cut(s) 278
AxyI CCTNAGG 1 cut(s) 1066
BaeGI GKGCMC 1 cut(s) 24
BalI TGGCCA 1 cut(s) 184
BanI GGYRCC 1 cut(s) 484
BanII GRGCYC 1 cut(s) 1000
BbsI GAAGAC 1 cut(s) 1046
Bbv12I GWGCWC 2 cut(s) 24, 1000
BbvI GCAGC 5 cut(s) 147, 226, 237, 341, 1105
BccI CCATC 6 cut(s) 110, 496, 829, 867, 1009, 1065
BceAI ACGGC 1 cut(s) 608
BciT130I CCWGG 4 cut(s) 205, 261, 619, 840
BciVI GTATCC 2 cut(s) 564, 740
BclI TGATCA 1 cut(s) 403
BcoDI GTCTC 1 cut(s) 142
BfaI CTAG 1 cut(s) 884
BfmI CTRYAG 2 cut(s) 161, 598
BfoI RGCGCY 1 cut(s) 231
BfuI GTATCC 2 cut(s) 564, 740
BisI GCNGC 5 cut(s) 161, 240, 251, 330, 1119
BlsI GCNGC 5 cut(s) 162, 241, 252, 331, 1120
BmcAI AGTACT 1 cut(s) 143
Bme1390I CCNGG 4 cut(s) 205, 261, 619, 840
Bme18I GGWCC 1 cut(s) 278
BmgT120I GGNCC 3 cut(s) 278, 427, 621
BmiI GGNNCC 3 cut(s) 279, 486, 819
BmrFI CCNGG 4 cut(s) 205, 261, 619, 840
BmrI ACTGGG 1 cut(s) 547
BmsI GCATC 1 cut(s) 696
BmtI GCTAGC 1 cut(s) 887
BmuI ACTGGG 1 cut(s) 547
BpiI GAAGAC 1 cut(s) 1046
BpuEI CTTGAG 1 cut(s) 166
Bsa29I ATCGAT 1 cut(s) 1225
BsaBI GATNNNNATC 2 cut(s) 1221, 1231
BsaJI CCNNGG 1 cut(s) 618
Bsc4I CCNNNNNNNGG 3 cut(s) 563, 1071, 1128
Bse1I ACTGG 2 cut(s) 542, 755
Bse21I CCTNAGG 1 cut(s) 1066
Bse3DI GCAATG 1 cut(s) 213
Bse8I GATNNNNATC 2 cut(s) 1221, 1231
BseBI CCWGG 4 cut(s) 205, 261, 619, 840
BseCI ATCGAT 1 cut(s) 1225
BseDI CCNNGG 1 cut(s) 618
BseGI GGATG 3 cut(s) 859, 1076, 1222
BseJI GATNNNNATC 2 cut(s) 1221, 1231
BseLI CCNNNNNNNGG 3 cut(s) 563, 1071, 1128
BseMI GCAATG 1 cut(s) 213
BseMII CTCAG 1 cut(s) 1080
BseNI ACTGG 2 cut(s) 542, 755
BseSI GKGCMC 1 cut(s) 24
BseXI GCAGC 5 cut(s) 147, 226, 237, 341, 1105
BseYI CCCAGC 2 cut(s) 293, 798
BsgI GTGCAG 1 cut(s) 225
BshFI GGCC 5 cut(s) 184, 304, 363, 428, 623
BshNI GGYRCC 1 cut(s) 484
BshVI ATCGAT 1 cut(s) 1225
BsiHKAI GWGCWC 2 cut(s) 24, 1000
BsiSI CCGG 1 cut(s) 290
BslFI GGGAC 1 cut(s) 291
BslI CCNNNNNNNGG 3 cut(s) 563, 1071, 1128
BsmAI GTCTC 1 cut(s) 142
BsmFI GGGAC 1 cut(s) 291
BsnI GGCC 5 cut(s) 184, 304, 363, 428, 623
Bsp1286I GDGCHC 2 cut(s) 24, 1000
Bsp1407I TGTACA 1 cut(s) 309
Bsp143I GATC 4 cut(s) 403, 775, 1222, 1232
BspACI CCGC 2 cut(s) 782, 905
BspANI GGCC 5 cut(s) 184, 304, 363, 428, 623
BspCNI CTCAG 1 cut(s) 1079
BspDI ATCGAT 1 cut(s) 1225
BspLI GGNNCC 3 cut(s) 279, 486, 819
BspMAI CTGCAG 1 cut(s) 165
BspOI GCTAGC 1 cut(s) 887
BspPI GGATC 1 cut(s) 1227
BspQI GCTCTTC 1 cut(s) 514
BspT107I GGYRCC 1 cut(s) 484
BsrBI CCGCTC 1 cut(s) 782
BsrDI GCAATG 1 cut(s) 213
BsrGI TGTACA 1 cut(s) 309
BsrI ACTGG 2 cut(s) 542, 755
BssECI CCNNGG 1 cut(s) 618
BssMI GATC 4 cut(s) 403, 775, 1222, 1232
BssNAI GTATAC 1 cut(s) 99
Bst1107I GTATAC 1 cut(s) 99
Bst2UI CCWGG 4 cut(s) 205, 261, 619, 840
Bst4CI ACNGT 5 cut(s) 19, 552, 599, 1135, 1164
Bst6I CTCTTC 1 cut(s) 514
BstAUI TGTACA 1 cut(s) 309
BstC8I GCNNGC 1 cut(s) 885
BstDEI CTNAG 1 cut(s) 1066
BstF5I GGATG 3 cut(s) 859, 1076, 1222
BstH2I RGCGCY 1 cut(s) 231
BstHHI GCGC 1 cut(s) 230
BstKTI GATC 4 cut(s) 406, 778, 1225, 1235
BstMAI GTCTC 1 cut(s) 142
BstMBI GATC 4 cut(s) 403, 775, 1222, 1232
BstMWI GCNNNNNNNGC 3 cut(s) 58, 236, 629
BstNI CCWGG 4 cut(s) 205, 261, 619, 840
BstNSI RCATGY 3 cut(s) 113, 222, 847
BstSCI CCNGG 4 cut(s) 203, 259, 617, 838
BstSFI CTRYAG 2 cut(s) 161, 598
BstSLI GKGCMC 1 cut(s) 24
BstV1I GCAGC 5 cut(s) 147, 226, 237, 341, 1105
BstV2I GAAGAC 1 cut(s) 1046
BstZ17I GTATAC 1 cut(s) 99
Bsu15I ATCGAT 1 cut(s) 1225
Bsu36I CCTNAGG 1 cut(s) 1066
BsuI GTATCC 2 cut(s) 564, 740
BsuRI GGCC 5 cut(s) 184, 304, 363, 428, 623
BsuTUI ATCGAT 1 cut(s) 1225
BtsCI GGATG 3 cut(s) 859, 1076, 1222
BtsI GCAGTG 2 cut(s) 56, 639
BtsIMutI CAGTG 2 cut(s) 56, 639
Cac8I GCNNGC 1 cut(s) 885
CaiI CAGNNNCTG 1 cut(s) 503
CfoI GCGC 1 cut(s) 230
Cfr13I GGNCC 3 cut(s) 278, 427, 621
ClaI ATCGAT 1 cut(s) 1225
Csp6I GTAC 5 cut(s) 107, 142, 310, 847, 1165
CviAII CATG 6 cut(s) 49, 110, 219, 544, 773, 844
CviQI GTAC 5 cut(s) 107, 142, 310, 847, 1165
DdeI CTNAG 1 cut(s) 1066
DpnI GATC 4 cut(s) 405, 777, 1224, 1234
DpnII GATC 4 cut(s) 403, 775, 1222, 1232
DraIII CACNNNGTG 1 cut(s) 484
EaeI YGGCCR 1 cut(s) 182
Eam1104I CTCTTC 1 cut(s) 514
EarI CTCTTC 1 cut(s) 514
Ecl136II GAGCTC 1 cut(s) 998
Eco24I GRGCYC 1 cut(s) 1000
Eco32I GATATC 1 cut(s) 580
Eco47I GGWCC 1 cut(s) 278
Eco53kI GAGCTC 1 cut(s) 998
Eco57I CTGAAG 1 cut(s) 230
Eco81I CCTNAGG 1 cut(s) 1066
EcoICRI GAGCTC 1 cut(s) 998
EcoRII CCWGG 4 cut(s) 203, 259, 617, 838
EcoRV GATATC 1 cut(s) 580
EcoT38I GRGCYC 1 cut(s) 1000
FaeI CATG 6 cut(s) 52, 113, 222, 547, 776, 847
FaqI GGGAC 1 cut(s) 291
FatI CATG 6 cut(s) 48, 109, 218, 543, 772, 843
FauI CCCGC 2 cut(s) 775, 898
FauNDI CATATG 1 cut(s) 179
FbaI TGATCA 1 cut(s) 403
FblI GTMKAC 1 cut(s) 98
Fnu4HI GCNGC 5 cut(s) 161, 240, 251, 330, 1119
FokI GGATG 3 cut(s) 846, 1083, 1229
FriOI GRGCYC 1 cut(s) 1000
Fsp4HI GCNGC 5 cut(s) 161, 240, 251, 330, 1119
FspBI CTAG 1 cut(s) 884
GlaI GCGC 1 cut(s) 229
GluI GCNGC 5 cut(s) 161, 240, 251, 330, 1119
GsaI CCCAGC 2 cut(s) 297, 802
HaeII RGCGCY 1 cut(s) 231
HaeIII GGCC 5 cut(s) 184, 304, 363, 428, 623
HapII CCGG 1 cut(s) 290
HhaI GCGC 1 cut(s) 230
Hin1II CATG 6 cut(s) 52, 113, 222, 547, 776, 847
Hin6I GCGC 1 cut(s) 228
HinP1I GCGC 1 cut(s) 228
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HinfI GANTC 5 cut(s) 85, 298, 500, 680, 910
HpaII CCGG 1 cut(s) 290
HphI GGTGA 4 cut(s) 18, 46, 578, 828
Hpy166II GTNNAC 5 cut(s) 22, 99, 310, 569, 856
Hpy188III TCNNGA 3 cut(s) 592, 1068, 1129
Hpy8I GTNNAC 5 cut(s) 22, 99, 310, 569, 856
HpyAV CCTTC 1 cut(s) 1202
HpyCH4III ACNGT 5 cut(s) 19, 552, 599, 1135, 1164
HpyCH4V TGCA 8 cut(s) 22, 163, 218, 242, 632, 645, 770, 918
HpyF10VI GCNNNNNNNGC 3 cut(s) 58, 236, 629
HpyF3I CTNAG 1 cut(s) 1066
Hsp92II CATG 6 cut(s) 52, 113, 222, 547, 776, 847
HspAI GCGC 1 cut(s) 228
Ksp22I TGATCA 1 cut(s) 403
Kzo9I GATC 4 cut(s) 403, 775, 1222, 1232
LguI GCTCTTC 1 cut(s) 514
LmnI GCTCC 1 cut(s) 1003
Lsp1109I GCAGC 5 cut(s) 147, 226, 237, 341, 1105
LweI GCATC 1 cut(s) 696
MaeI CTAG 1 cut(s) 884
MaeIII GTNAC 5 cut(s) 34, 340, 476, 634, 718
MalI GATC 4 cut(s) 405, 777, 1224, 1234
MbiI CCGCTC 1 cut(s) 782
MboI GATC 4 cut(s) 403, 775, 1222, 1232
MboII GAAGA 4 cut(s) 483, 501, 721, 1046
MfeI CAATTG 1 cut(s) 393
MhlI GDGCHC 2 cut(s) 24, 1000
MlsI TGGCCA 1 cut(s) 184
MluCI AATT 5 cut(s) 393, 525, 561, 725, 956
MluNI TGGCCA 1 cut(s) 184
MnlI CCTC 8 cut(s) 84, 229, 268, 440, 567, 733, 844, 1075
Mox20I TGGCCA 1 cut(s) 184
MscI TGGCCA 1 cut(s) 184
MseI TTAA 1 cut(s) 1061
MslI CAYNNNNRTG 1 cut(s) 114
Msp20I TGGCCA 1 cut(s) 184
MspA1I CMGCKG 2 cut(s) 250, 1118
MspI CCGG 1 cut(s) 290
MspR9I CCNGG 4 cut(s) 205, 261, 619, 840
MunI CAATTG 1 cut(s) 393
MvaI CCWGG 4 cut(s) 205, 261, 619, 840
MwoI GCNNNNNNNGC 3 cut(s) 58, 236, 629
NdeI CATATG 1 cut(s) 179
NdeII GATC 4 cut(s) 403, 775, 1222, 1232
NheI GCTAGC 1 cut(s) 883
NlaIII CATG 6 cut(s) 52, 113, 222, 547, 776, 847
NlaIV GGNNCC 3 cut(s) 279, 486, 819
NmeAIII GCCGAG 1 cut(s) 852
NmuCI GTSAC 5 cut(s) 34, 340, 476, 634, 718
NspI RCATGY 3 cut(s) 113, 222, 847
PciSI GCTCTTC 1 cut(s) 514
PfeI GAWTC 5 cut(s) 85, 298, 500, 680, 910
PkrI GCNGC 5 cut(s) 162, 241, 252, 331, 1120
PsiI TTATAA 1 cut(s) 524
Psp124BI GAGCTC 1 cut(s) 1000
Psp6I CCWGG 4 cut(s) 203, 259, 617, 838
PspFI CCCAGC 2 cut(s) 293, 798
PspGI CCWGG 4 cut(s) 203, 259, 617, 838
PspN4I GGNNCC 3 cut(s) 279, 486, 819
PspPI GGNCC 3 cut(s) 278, 427, 621
PstI CTGCAG 1 cut(s) 165
PstNI CAGNNNCTG 1 cut(s) 503
PvuII CAGCTG 2 cut(s) 250, 1118
RsaI GTAC 5 cut(s) 108, 143, 311, 848, 1166
RsaNI GTAC 5 cut(s) 107, 142, 310, 847, 1165
RseI CAYNNNNRTG 1 cut(s) 114
SacI GAGCTC 1 cut(s) 1000
SapI GCTCTTC 1 cut(s) 514
SaqAI TTAA 1 cut(s) 1061
SatI GCNGC 5 cut(s) 161, 240, 251, 330, 1119
Sau3AI GATC 4 cut(s) 403, 775, 1222, 1232
Sau96I GGNCC 3 cut(s) 278, 427, 621
ScaI AGTACT 1 cut(s) 143
ScrFI CCNGG 4 cut(s) 205, 261, 619, 840
SduI GDGCHC 2 cut(s) 24, 1000
SfaNI GCATC 1 cut(s) 696
SfcI CTRYAG 2 cut(s) 161, 598
SinI GGWCC 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 114
SmlI CTYRAG 1 cut(s) 145
SmoI CTYRAG 1 cut(s) 145
Sse9I AATT 5 cut(s) 393, 525, 561, 725, 956
SsiI CCGC 2 cut(s) 782, 905
SspI AATATT 1 cut(s) 420
SspMI CTAG 1 cut(s) 884
SstI GAGCTC 1 cut(s) 1000
StyD4I CCNGG 4 cut(s) 203, 259, 617, 838
TaaI ACNGT 5 cut(s) 19, 552, 599, 1135, 1164
TaqI TCGA 5 cut(s) 273, 559, 778, 1225, 1231
TaqII GACCGA 1 cut(s) 1218
TasI AATT 5 cut(s) 393, 525, 561, 725, 956
TatI WGTACW 3 cut(s) 141, 309, 846
TfiI GAWTC 5 cut(s) 85, 298, 500, 680, 910
Tru1I TTAA 1 cut(s) 1061
Tru9I TTAA 1 cut(s) 1061
TscAI CASTG 2 cut(s) 63, 639
TseFI GTSAC 5 cut(s) 34, 340, 476, 634, 718
TseI GCWGC 5 cut(s) 160, 239, 250, 329, 1118
Tsp45I GTSAC 5 cut(s) 34, 340, 476, 634, 718
TspDTI ATGAA 1 cut(s) 1008
TspRI CASTG 2 cut(s) 63, 639
VneI GTGCAC 1 cut(s) 20
VpaK11BI GGWCC 1 cut(s) 278
XapI RAATTY 2 cut(s) 725, 956
XceI RCATGY 3 cut(s) 113, 222, 847
XmiI GTMKAC 1 cut(s) 98
XspI CTAG 1 cut(s) 884
ZrmI AGTACT 1 cut(s) 143
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.