pycom09g08210

7-dehydrocholesterol reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0012401
Physical Location & Seq
Forward (+)
6300276 .. 6305656
5381 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g08210.3

Sequence Viewer

Length: 1302 bp
ATGGGGGAGACGAAGACCGTACATTCGCCATTGGTGACGTACGCTTCCATGCTATCGCTGCTTTCTCTCTGCCCTCCCTTTGTAATTCTACTATGGTATACAATGGTACATGCTGATGGCTCTATTCTGCAAACTTGGGATCACTTGAGGCAGCATGGACTGCAAGGGTTCATCGAAATCTGGCCCAGACCTACTGCCACTGCATGGAAGATTATTGCCTGTTACGGTGCTTTTGAGGCTGCACTTCAGCTTCTTTTACCTGGAAAAAGGGTTGAGGGACCAATATCTCCAGCAGGGAATCGGCCTGTATACAAGGCAAATGGTATGGCAGCCTATTTTGTAACGTTGACTACCTACCTTGGCCTTTGGTGGTTTGGGATATTCAACCCTACAGTTGTTTATGATCATCTGGGAGAAATATTTTCAGCACTCATTTTCGGAAGCTTCATTTTTTGTATTTTCCTGTACATAAAAGGCCATGTGGCACCATCTTCCACTGATTCTGGATCATCTGGGAATGCAATAATTGATTTCTATTGGGGCATGGAATTGTATCCTCGAATTGGCAAATACTTTGATATCAAAGTATTCACAAACTGCAGATTTGGGATGATGTCTTGGGCAGTTCTTGCTGTGACTTATTGCATAAAGCAGTATGAAGTCAATGGGAGAGTAGCTGACTCAATGCTTGTGAATACTATATTGATGCTGGTGTATGTCACAAAGTTTTTCTGGTGGGAGGCTGGATACTGGAATACAATGGATATCGCACATCGATCGAGCGGGATTCTATATTTGCTGGGGTTGCTTGGTCTGGGTTCCATCTATTTACACTTCTCCCGGCATGTACCTGGTCAACCATCCTGTAAATCTTGGTACTCAGCACTTTTTATTCTGGTGGCCGGAATCCTTTGCATATACATCAACTATGATTGTGACAGGCAAAGGCAAGAATTTCGCAGAACTAATGGAAAATGCTTGGTTTGGGGGAGAGCTCCATCAAAGATAGTCGCTTCATACACTACCACATCTGGGGAAACAAAGACAAGCCTTCTTCTAACCTCAGGATGGTGGGGTTTATCTCGTCACTTCCATTATGTGCCAGAAATATCAGCTGCCTTTTTCTGGACTGTTCCAGCCCTCTTCAATCATTTTCTACCTTACTTCTACGTGATTTTCCTGACAATCCTTCTGCTTGACCGAGCAAAGAGGGATGATGACCGATGTCGCACTAAGTACGGCAAATACTGGAAGTCATATTGTCAGAAGGTTCCCTACAGGGTTGTTCCCGGAATCTACTGA

Protein Analysis

434

Amino Acids

49.56

Weight (kDa)

9.13

Isoelectric Point (pI)

35.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012401)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G50430 AT1G50430
fragaria_vesca FvH4_6g37020 FvH4_6g37020 FvH4_6g37020
malus_domestica MD09G1164900.v1.1 MD17G1163300.v1.1
prunus_persica Prupe.3G022300_v2.0.a1 Prupe.3G022300_v2.0.a1
pyrus_communis pycom09g08210
rosa_chinensis RchiOBHm_Chr2g0149571
rosa_laevigata RLG00000020424
rosa_multiflora Rmu_sc0006867.1_g000021
rosa_roxburghii Rroxscaffold_2G00097980
rosa_rugosa Rorug02G0412400
rosa_samantha Rh2AG473000 Rh2BG484900 Rh2DG494100
rosa_wichuraiana Rw2G038550

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 484
AccB7I CCANNNNNTGG 1 cut(s) 204
AccBSI CCGCTC 1 cut(s) 783
AccI GTMKAC 2 cut(s) 98, 309
AciI CCGC 1 cut(s) 783
AclI AACGTT 1 cut(s) 344
AclWI GGATC 2 cut(s) 147, 514
AcoI YGGCCR 1 cut(s) 900
AcsI RAATTY 1 cut(s) 953
AcuI CTGAAG 1 cut(s) 230
AfaI GTAC 7 cut(s) 21, 41, 108, 467, 849, 878, 1238
AfiI CCNNNNNNNGG 5 cut(s) 204, 563, 1032, 1068, 1125
AgsI TTSAA 2 cut(s) 385, 1147
AjnI CCWGG 2 cut(s) 259, 850
AluBI AGCT 5 cut(s) 250, 444, 677, 995, 1115
AluI AGCT 5 cut(s) 250, 444, 677, 995, 1115
Alw21I GWGCWC 1 cut(s) 997
Alw26I GTCTC 1 cut(s) 2
AlwI GGATC 2 cut(s) 147, 514
AoxI GGCC 5 cut(s) 182, 302, 361, 475, 900
ApeKI GCWGC 5 cut(s) 58, 151, 239, 329, 1115
ApoI RAATTY 1 cut(s) 953
ArsI GACNNNNNNTTYG 2 cut(s) 7, 39
AspS9I GGNCC 2 cut(s) 183, 278
AsuC2I CCSGG 2 cut(s) 841, 1290
AsuHPI GGTGA 1 cut(s) 46
AvaII GGWCC 1 cut(s) 278
AxyI CCTNAGG 1 cut(s) 1063
BanI GGYRCC 1 cut(s) 484
BanII GRGCYC 1 cut(s) 997
BarI GAAGNNNNNNTAC 2 cut(s) 1259, 1291
BbsI GAAGAC 1 cut(s) 20
Bbv12I GWGCWC 1 cut(s) 997
BbvI GCAGC 5 cut(s) 45, 163, 226, 341, 1102
BccI CCATC 6 cut(s) 110, 496, 830, 868, 1006, 1062
BceAI ACGGC 1 cut(s) 1255
BcgI CGANNNNNNTGC 4 cut(s) 759, 793, 938, 972
BciT130I CCWGG 2 cut(s) 261, 852
BciVI GTATCC 2 cut(s) 564, 740
BclI TGATCA 1 cut(s) 403
BcnI CCSGG 2 cut(s) 841, 1290
BcoDI GTCTC 1 cut(s) 2
BfmI CTRYAG 3 cut(s) 390, 598, 1276
BfuI GTATCC 2 cut(s) 564, 740
BisI GCNGC 5 cut(s) 59, 152, 240, 330, 1116
BlsI GCNGC 5 cut(s) 60, 153, 241, 331, 1117
Bme1390I CCNGG 4 cut(s) 261, 841, 852, 1290
Bme18I GGWCC 1 cut(s) 278
BmgT120I GGNCC 2 cut(s) 183, 278
BmiI GGNNCC 4 cut(s) 279, 486, 820, 1272
BmrFI CCNGG 4 cut(s) 261, 841, 852, 1290
BmsI GCATC 1 cut(s) 696
BoxI GACNNNNGTC 1 cut(s) 1224
BpiI GAAGAC 1 cut(s) 20
BpmI CTGGAG 1 cut(s) 273
BpuEI CTTGAG 1 cut(s) 166
BpuMI CCSGG 2 cut(s) 841, 1290
Bsa29I ATCGAT 1 cut(s) 775
BsaAI YACGTR 1 cut(s) 1171
BsaJI CCNNGG 1 cut(s) 358
Bsc4I CCNNNNNNNGG 5 cut(s) 204, 563, 1032, 1068, 1125
Bse1I ACTGG 2 cut(s) 755, 1253
Bse21I CCTNAGG 1 cut(s) 1063
BseBI CCWGG 2 cut(s) 261, 852
BseCI ATCGAT 1 cut(s) 775
BseDI CCNNGG 1 cut(s) 358
BseGI GGATG 4 cut(s) 615, 860, 1073, 1219
BseLI CCNNNNNNNGG 5 cut(s) 204, 563, 1032, 1068, 1125
BseMII CTCAG 2 cut(s) 894, 1077
BseNI ACTGG 2 cut(s) 755, 1253
BseXI GCAGC 5 cut(s) 45, 163, 226, 341, 1102
BseYI CCCAGC 1 cut(s) 799
BsgI GTGCAG 1 cut(s) 225
Bsh1285I CGRYCG 1 cut(s) 779
BshFI GGCC 5 cut(s) 184, 304, 363, 477, 902
BshNI GGYRCC 1 cut(s) 484
BshVI ATCGAT 1 cut(s) 775
BsiEI CGRYCG 1 cut(s) 779
BsiHKAI GWGCWC 1 cut(s) 997
BsiSI CCGG 3 cut(s) 841, 903, 1290
BsiWI CGTACG 1 cut(s) 39
BslFI GGGAC 1 cut(s) 291
BslI CCNNNNNNNGG 5 cut(s) 204, 563, 1032, 1068, 1125
BsmAI GTCTC 1 cut(s) 2
BsmBI CGTCTC 1 cut(s) 2
BsmFI GGGAC 1 cut(s) 291
BsmI GAATGC 1 cut(s) 523
BsnI GGCC 5 cut(s) 184, 304, 363, 477, 902
Bsp1286I GDGCHC 1 cut(s) 997
Bsp1407I TGTACA 1 cut(s) 465
Bsp143I GATC 4 cut(s) 139, 403, 506, 776
BspACI CCGC 1 cut(s) 783
BspANI GGCC 5 cut(s) 184, 304, 363, 477, 902
BspCNI CTCAG 2 cut(s) 893, 1076
BspDI ATCGAT 1 cut(s) 775
BspLI GGNNCC 4 cut(s) 279, 486, 820, 1272
BspMAI CTGCAG 1 cut(s) 602
BspPI GGATC 2 cut(s) 147, 514
BspT107I GGYRCC 1 cut(s) 484
BsrBI CCGCTC 1 cut(s) 783
BsrGI TGTACA 1 cut(s) 465
BsrI ACTGG 2 cut(s) 755, 1253
BssECI CCNNGG 1 cut(s) 358
BssMI GATC 4 cut(s) 139, 403, 506, 776
BssNAI GTATAC 2 cut(s) 99, 310
BssT1I CCWWGG 1 cut(s) 358
Bst1107I GTATAC 2 cut(s) 99, 310
Bst2UI CCWGG 2 cut(s) 261, 852
Bst4CI ACNGT 4 cut(s) 19, 227, 394, 1132
Bst6I CTCTTC 1 cut(s) 1148
BstAPI GCANNNNNTGC 2 cut(s) 160, 629
BstAUI TGTACA 1 cut(s) 465
BstBAI YACGTR 1 cut(s) 1171
BstDEI CTNAG 3 cut(s) 880, 1063, 1233
BstF5I GGATG 4 cut(s) 615, 860, 1073, 1219
BstKTI GATC 4 cut(s) 142, 406, 509, 779
BstMAI GTCTC 1 cut(s) 2
BstMBI GATC 4 cut(s) 139, 403, 506, 776
BstMCI CGRYCG 1 cut(s) 779
BstMWI GCNNNNNNNGC 5 cut(s) 58, 160, 236, 629, 805
BstNI CCWGG 2 cut(s) 261, 852
BstNSI RCATGY 2 cut(s) 113, 848
BstPAI GACNNNNGTC 1 cut(s) 1224
BstSCI CCNGG 4 cut(s) 259, 839, 850, 1288
BstSFI CTRYAG 3 cut(s) 390, 598, 1276
BstV1I GCAGC 5 cut(s) 45, 163, 226, 341, 1102
BstV2I GAAGAC 1 cut(s) 20
BstZ17I GTATAC 2 cut(s) 99, 310
Bsu15I ATCGAT 1 cut(s) 775
Bsu36I CCTNAGG 1 cut(s) 1063
BsuI GTATCC 2 cut(s) 564, 740
BsuRI GGCC 5 cut(s) 184, 304, 363, 477, 902
BsuTUI ATCGAT 1 cut(s) 775
BtsCI GGATG 4 cut(s) 615, 860, 1073, 1219
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 2 cut(s) 198, 495
Cfr13I GGNCC 2 cut(s) 183, 278
ClaI ATCGAT 1 cut(s) 775
CsiI ACCWGGT 1 cut(s) 850
Csp6I GTAC 7 cut(s) 20, 40, 107, 466, 848, 877, 1237
CviAII CATG 7 cut(s) 49, 110, 155, 204, 479, 544, 845
CviQI GTAC 7 cut(s) 20, 40, 107, 466, 848, 877, 1237
DdeI CTNAG 3 cut(s) 880, 1063, 1233
DpnI GATC 4 cut(s) 141, 405, 508, 778
DpnII GATC 4 cut(s) 139, 403, 506, 776
EaeI YGGCCR 1 cut(s) 900
Eam1104I CTCTTC 1 cut(s) 1148
EarI CTCTTC 1 cut(s) 1148
Ecl136II GAGCTC 1 cut(s) 995
Eco130I CCWWGG 1 cut(s) 358
Eco24I GRGCYC 1 cut(s) 997
Eco32I GATATC 2 cut(s) 580, 766
Eco47I GGWCC 1 cut(s) 278
Eco53kI GAGCTC 1 cut(s) 995
Eco57I CTGAAG 1 cut(s) 230
Eco81I CCTNAGG 1 cut(s) 1063
EcoICRI GAGCTC 1 cut(s) 995
EcoRII CCWGG 2 cut(s) 259, 850
EcoRV GATATC 2 cut(s) 580, 766
EcoT14I CCWWGG 1 cut(s) 358
EcoT38I GRGCYC 1 cut(s) 997
ErhI CCWWGG 1 cut(s) 358
Esp3I CGTCTC 1 cut(s) 2
FaeI CATG 7 cut(s) 52, 113, 158, 207, 482, 547, 848
FaqI GGGAC 1 cut(s) 291
FatI CATG 7 cut(s) 48, 109, 154, 203, 478, 543, 844
FauI CCCGC 1 cut(s) 776
FbaI TGATCA 1 cut(s) 403
FblI GTMKAC 2 cut(s) 98, 309
Fnu4HI GCNGC 5 cut(s) 59, 152, 240, 330, 1116
FokI GGATG 4 cut(s) 622, 847, 1080, 1226
FriOI GRGCYC 1 cut(s) 997
Fsp4HI GCNGC 5 cut(s) 59, 152, 240, 330, 1116
GluI GCNGC 5 cut(s) 59, 152, 240, 330, 1116
GsaI CCCAGC 1 cut(s) 803
GsuI CTGGAG 1 cut(s) 273
HaeIII GGCC 5 cut(s) 184, 304, 363, 477, 902
HapII CCGG 3 cut(s) 841, 903, 1290
Hin1II CATG 7 cut(s) 52, 113, 158, 207, 482, 547, 848
HincII GTYRAC 2 cut(s) 348, 857
HindII GTYRAC 2 cut(s) 348, 857
HindIII AAGCTT 1 cut(s) 442
HinfI GANTC 6 cut(s) 298, 500, 680, 787, 906, 1293
HpaII CCGG 3 cut(s) 841, 903, 1290
HphI GGTGA 1 cut(s) 46
Hpy166II GTNNAC 4 cut(s) 99, 310, 348, 857
Hpy188I TCNGA 2 cut(s) 440, 1266
Hpy188III TCNNGA 4 cut(s) 504, 1065, 1126, 1180
Hpy8I GTNNAC 4 cut(s) 99, 310, 348, 857
HpyAV CCTTC 3 cut(s) 1061, 1199, 1261
HpyCH4III ACNGT 4 cut(s) 19, 227, 394, 1132
HpyCH4IV ACGT 3 cut(s) 38, 344, 1170
HpyCH4V TGCA 8 cut(s) 130, 163, 203, 242, 521, 600, 645, 915
HpyF10VI GCNNNNNNNGC 5 cut(s) 58, 160, 236, 629, 805
HpyF3I CTNAG 3 cut(s) 880, 1063, 1233
HpySE526I ACGT 3 cut(s) 38, 344, 1170
Hsp92II CATG 7 cut(s) 52, 113, 158, 207, 482, 547, 848
Ksp22I TGATCA 1 cut(s) 403
Kzo9I GATC 4 cut(s) 139, 403, 506, 776
LmnI GCTCC 1 cut(s) 1000
Lsp1109I GCAGC 5 cut(s) 45, 163, 226, 341, 1102
LweI GCATC 1 cut(s) 696
MabI ACCWGGT 1 cut(s) 850
MaeII ACGT 3 cut(s) 38, 344, 1170
MaeIII GTNAC 7 cut(s) 34, 221, 340, 634, 718, 935, 1085
MalI GATC 4 cut(s) 141, 405, 508, 778
MbiI CCGCTC 1 cut(s) 783
MboI GATC 4 cut(s) 139, 403, 506, 776
MboII GAAGA 5 cut(s) 25, 220, 483, 1046, 1135
MhlI GDGCHC 1 cut(s) 997
MluCI AATT 5 cut(s) 84, 525, 548, 561, 953
MlyI GAGTC 1 cut(s) 674
MnlI CCTC 9 cut(s) 84, 141, 229, 268, 567, 733, 1072, 1151, 1203
MslI CAYNNNNRTG 1 cut(s) 114
MspA1I CMGCKG 1 cut(s) 1115
MspI CCGG 3 cut(s) 841, 903, 1290
MspR9I CCNGG 4 cut(s) 261, 841, 852, 1290
Mva1269I GAATGC 1 cut(s) 523
MvaI CCWGG 2 cut(s) 261, 852
MwoI GCNNNNNNNGC 5 cut(s) 58, 160, 236, 629, 805
NciI CCSGG 2 cut(s) 841, 1290
NdeII GATC 4 cut(s) 139, 403, 506, 776
NlaIII CATG 7 cut(s) 52, 113, 158, 207, 482, 547, 848
NlaIV GGNNCC 4 cut(s) 279, 486, 820, 1272
NmuCI GTSAC 5 cut(s) 34, 634, 718, 935, 1085
NspI RCATGY 2 cut(s) 113, 848
PctI GAATGC 1 cut(s) 523
PfeI GAWTC 5 cut(s) 298, 500, 787, 906, 1293
Pfl23II CGTACG 1 cut(s) 39
PflMI CCANNNNNTGG 1 cut(s) 204
PfoI TCCNGGA 1 cut(s) 1288
PkrI GCNGC 5 cut(s) 60, 153, 241, 331, 1117
Ple19I CGATCG 1 cut(s) 779
PleI GAGTC 1 cut(s) 674
PpsI GAGTC 1 cut(s) 674
Ppu21I YACGTR 1 cut(s) 1171
PshAI GACNNNNGTC 1 cut(s) 1224
Psp124BI GAGCTC 1 cut(s) 997
Psp1406I AACGTT 1 cut(s) 344
Psp6I CCWGG 2 cut(s) 259, 850
PspFI CCCAGC 1 cut(s) 799
PspGI CCWGG 2 cut(s) 259, 850
PspLI CGTACG 1 cut(s) 39
PspN4I GGNNCC 4 cut(s) 279, 486, 820, 1272
PspPI GGNCC 2 cut(s) 183, 278
PstI CTGCAG 1 cut(s) 602
PvuI CGATCG 1 cut(s) 779
PvuII CAGCTG 1 cut(s) 1115
RsaI GTAC 7 cut(s) 21, 41, 108, 467, 849, 878, 1238
RsaNI GTAC 7 cut(s) 20, 40, 107, 466, 848, 877, 1237
RseI CAYNNNNRTG 1 cut(s) 114
SacI GAGCTC 1 cut(s) 997
SatI GCNGC 5 cut(s) 59, 152, 240, 330, 1116
Sau3AI GATC 4 cut(s) 139, 403, 506, 776
Sau96I GGNCC 2 cut(s) 183, 278
SchI GAGTC 1 cut(s) 674
ScrFI CCNGG 4 cut(s) 261, 841, 852, 1290
SduI GDGCHC 1 cut(s) 997
SexAI ACCWGGT 1 cut(s) 850
SfaNI GCATC 1 cut(s) 696
SfcI CTRYAG 3 cut(s) 390, 598, 1276
SinI GGWCC 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 114
SmlI CTYRAG 1 cut(s) 145
SmoI CTYRAG 1 cut(s) 145
Sse9I AATT 5 cut(s) 84, 525, 548, 561, 953
SsiI CCGC 1 cut(s) 783
SspI AATATT 1 cut(s) 420
SstI GAGCTC 1 cut(s) 997
StyD4I CCNGG 4 cut(s) 259, 839, 850, 1288
StyI CCWWGG 1 cut(s) 358
TaaI ACNGT 4 cut(s) 19, 227, 394, 1132
TaiI ACGT 3 cut(s) 41, 347, 1173
TaqI TCGA 4 cut(s) 174, 559, 775, 779
TaqII GACCGA 2 cut(s) 1215, 1236
TasI AATT 5 cut(s) 84, 525, 548, 561, 953
TatI WGTACW 1 cut(s) 465
TfiI GAWTC 5 cut(s) 298, 500, 787, 906, 1293
TscAI CASTG 2 cut(s) 205, 502
TseFI GTSAC 5 cut(s) 34, 634, 718, 935, 1085
TseI GCWGC 5 cut(s) 58, 151, 239, 329, 1115
Tsp45I GTSAC 5 cut(s) 34, 634, 718, 935, 1085
TspDTI ATGAA 4 cut(s) 160, 436, 672, 1005
TspRI CASTG 2 cut(s) 205, 502
Van91I CCANNNNNTGG 1 cut(s) 204
VpaK11BI GGWCC 1 cut(s) 278
XapI RAATTY 1 cut(s) 953
XceI RCATGY 2 cut(s) 113, 848
XmiI GTMKAC 2 cut(s) 98, 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.