Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0012401

Physical Location & Seq

Genomic Coordinates Reverse (-)

16155517 .. 16161448

Gene Structure 5932 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1163300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1305 bp

ATGGGGGAGACAAAGACCGTACATTCCCCATTGGTGACGTACGCTTCCATGCTATCGCTGCTTTCTCTCTGCCCTCCCTTTGTAATTCTACTATGGTATACAATGGTACATGCTGATGGCTCCATTTTGCAAACTTGGGATTACTTGAGGCAGCATGGACTGCAGGGGTTTATCAAAATATGGCCAATACCTACTGCCACTGCATGGAAGATTATTGCCTGTTACGGTGCTTTTGAGGCTGCACTTCAGCTTCTTTTACCTGGAAAAAGGGTTGAGGGGCCAATATCTCCAGCTGGGAATCGACCTGTCTACAAGGCAAATGGTATGGCGGCCTATTTTGTAACGTTGACTACCTACCTTGGCCTTTGGTGGTTCGGGATATTCAACCCTACAGTTGTTTATGATCATCTGGGAGAAATATTTTCTACCCTCATTTTTGGAAGTTTCATCTTTTGTATTTTCCTGTACATAAAAGGCCATGTGGCACCATCTTCCACTGATTCTGGATCTTCTGGGAATGCAATAATTGACTTCTATTGGGGCTTGGAGCTGTATCCACGAATTGGTAAATACTTCGATATCAAAGTTTTCACAAACTGCAGATTTGGGATGATGTCCTGGGCAGTTCTTGCTGTGACCTATTGCATAAAGCAGTATGAAGTTAATGGGAAAGTAGCTGACTCAATGCTTGTGAATACTATATTGATGCTGGTGTATGTCACAAAGTTTTTCTGGTGGGAGGCTGGATACTGGAATACAATGGATATTGCACATGATCGAGCGGGATTTTATATTTGCTGGGGTTGCTTGGTCTGGGTTCCATCTATTTATACATCTCCTGGCATGTACCTGGTCAACCATCCTATAAATCTTGGAACTCAGCTAGCACTTTTTATTCTGGTGGCCGGGATCCTTTGCATATACATCAACTATGATTGTGACAGGCAAAGGCAAGAATTTCGGAGAACAAATGGCAAATGCTTGGTTTGGGGGAGAGCTCCATCAAAGATAGTTGCTTCATACACTACCACATCCGGGGAAACAAAGACAAGCCTTCTTCTAACCTCAGGATGGTGGGGTTTATCACGTCATTTCCACTACGTGCCAGAAATATCAGCTGCCTTCTTCTGGACTGTTCCAGCTCTTTTCAATCATTTTCTACCATACTTCTATGTGGTTTTCCTGACAATCCTTCTGCTTGACCGAGCCAAGAGGGATGATGACCGATGTCGCTCCAAGTATGGCAAATACTGGAAGTTATATTGTCAGAAGGTTTCCTACAGGGTCATCCCCGGAATCTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0005789 GO:0005794 GO:0005886 GO:0006629 GO:0006694 GO:0008150 GO:0008152 GO:0008202 GO:0008610 GO:0009058 GO:0009918 GO:0010817 GO:0012505 GO:0016020 GO:0016128 GO:0016129 GO:0016131 GO:0016132 GO:0016491 GO:0016627 GO:0031984 GO:0042175 GO:0042445 GO:0042446 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0055114 GO:0065007 GO:0065008 GO:0071704 GO:0071944 GO:0098827 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00100 ko01100 ko01110 map00100 map01100 map01110

Pfam Domains

Protein Families

ERG4_ERG24

Protein Analysis

435

Amino Acids

49.72

Weight (kDa)

8.97

Isoelectric Point (pI)

31.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ERG4_ERG24	PF01222	41 - 434	4e-86	Ergosterol biosynthesis ERG4/ERG24 family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012401)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G50430 AT1G50430
fragaria_vesca	FvH4_6g37020 FvH4_6g37020 FvH4_6g37020
malus_domestica	MD09G1164900.v1.1 MD17G1163300.v1.1
prunus_persica	Prupe.3G022300_v2.0.a1 Prupe.3G022300_v2.0.a1
pyrus_communis	pycom09g08210
rosa_chinensis	RchiOBHm_Chr2g0149571
rosa_laevigata	RLG00000020424
rosa_multiflora	Rmu_sc0006867.1_g000021
rosa_roxburghii	Rroxscaffold_2G00097980
rosa_rugosa	Rorug02G0412400
rosa_samantha	Rh2AG473000 Rh2BG484900 Rh2DG494100
rosa_wichuraiana	Rw2G038550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	484
AccB7I	CCANNNNNTGG	2 cut(s)	204, 563
AccBSI	CCGCTC	1 cut(s)	782
AccI	GTMKAC	2 cut(s)	98, 309
AciI	CCGC	2 cut(s)	329, 782
AclI	AACGTT	1 cut(s)	344
AclWI	GGATC	3 cut(s)	514, 904, 917
AcoI	YGGCCR	2 cut(s)	182, 903
AcsI	RAATTY	1 cut(s)	956
AcuI	CTGAAG	1 cut(s)	230
AdeI	CACNNNGTG	1 cut(s)	1102
AfaI	GTAC	5 cut(s)	21, 41, 108, 467, 848
AfiI	CCNNNNNNNGG	5 cut(s)	204, 563, 1035, 1071, 1128
AgsI	TTSAA	2 cut(s)	385, 1150
AjiI	CACGTC	1 cut(s)	1088
AjnI	CCWGG	4 cut(s)	259, 617, 838, 849
AluBI	AGCT	8 cut(s)	250, 293, 550, 677, 883, 998, 1118, 1142
AluI	AGCT	8 cut(s)	250, 293, 550, 677, 883, 998, 1118, 1142
Alw21I	GWGCWC	1 cut(s)	1000
Alw26I	GTCTC	1 cut(s)	2
AlwI	GGATC	3 cut(s)	514, 904, 917
AoxI	GGCC	6 cut(s)	182, 278, 330, 361, 475, 903
ApeKI	GCWGC	4 cut(s)	58, 151, 239, 1118
ApoI	RAATTY	1 cut(s)	956
AspS9I	GGNCC	1 cut(s)	278
AsuC2I	CCSGG	3 cut(s)	907, 1036, 1293
AsuHPI	GGTGA	1 cut(s)	46
AsuNHI	GCTAGC	1 cut(s)	883
AxyI	CCTNAGG	1 cut(s)	1066
BalI	TGGCCA	1 cut(s)	184
BamHI	GGATCC	1 cut(s)	909
BanI	GGYRCC	1 cut(s)	484
BanII	GRGCYC	1 cut(s)	1000
BarI	GAAGNNNNNNTAC	2 cut(s)	1262, 1294
Bbv12I	GWGCWC	1 cut(s)	1000
BbvI	GCAGC	4 cut(s)	45, 163, 226, 1105
BccI	CCATC	6 cut(s)	110, 496, 829, 867, 1009, 1065
BcgI	CGANNNNNNTGC	2 cut(s)	941, 975
BciT130I	CCWGG	4 cut(s)	261, 619, 840, 851
BciVI	GTATCC	2 cut(s)	564, 740
BclI	TGATCA	1 cut(s)	403
BcnI	CCSGG	3 cut(s)	907, 1036, 1293
BcoDI	GTCTC	1 cut(s)	2
BfaI	CTAG	1 cut(s)	884
BfmI	CTRYAG	4 cut(s)	161, 390, 598, 1279
BfuI	GTATCC	2 cut(s)	564, 740
BisI	GCNGC	5 cut(s)	59, 152, 240, 330, 1119
BlsI	GCNGC	5 cut(s)	60, 153, 241, 331, 1120
Bme1390I	CCNGG	7 cut(s)	261, 619, 840, 851, 907, 1036, 1293
BmgBI	CACGTC	1 cut(s)	1088
BmgT120I	GGNCC	1 cut(s)	278
BmiI	GGNNCC	5 cut(s)	121, 279, 486, 819, 911
BmrFI	CCNGG	7 cut(s)	261, 619, 840, 851, 907, 1036, 1293
BmsI	GCATC	1 cut(s)	696
BmtI	GCTAGC	1 cut(s)	887
BoxI	GACNNNNGTC	1 cut(s)	1227
BpmI	CTGGAG	1 cut(s)	273
BpuEI	CTTGAG	1 cut(s)	166
BpuMI	CCSGG	3 cut(s)	907, 1036, 1293
BsaAI	YACGTR	1 cut(s)	1102
BsaJI	CCNNGG	4 cut(s)	358, 618, 1035, 1291
Bsc4I	CCNNNNNNNGG	5 cut(s)	204, 563, 1035, 1071, 1128
Bse1I	ACTGG	2 cut(s)	755, 1256
Bse21I	CCTNAGG	1 cut(s)	1066
BseBI	CCWGG	4 cut(s)	261, 619, 840, 851
BseDI	CCNNGG	4 cut(s)	358, 618, 1035, 1291
BseGI	GGATG	6 cut(s)	615, 859, 1031, 1076, 1222, 1287
BseLI	CCNNNNNNNGG	5 cut(s)	204, 563, 1035, 1071, 1128
BseMII	CTCAG	2 cut(s)	893, 1080
BseNI	ACTGG	2 cut(s)	755, 1256
BseXI	GCAGC	4 cut(s)	45, 163, 226, 1105
BseYI	CCCAGC	2 cut(s)	293, 798
BsgI	GTGCAG	1 cut(s)	225
BshFI	GGCC	6 cut(s)	184, 280, 332, 363, 477, 905
BshNI	GGYRCC	1 cut(s)	484
BsiHKAI	GWGCWC	1 cut(s)	1000
BsiSI	CCGG	3 cut(s)	906, 1035, 1293
BsiWI	CGTACG	1 cut(s)	39
BslI	CCNNNNNNNGG	5 cut(s)	204, 563, 1035, 1071, 1128
BsmAI	GTCTC	1 cut(s)	2
BsmI	GAATGC	1 cut(s)	523
BsnI	GGCC	6 cut(s)	184, 280, 332, 363, 477, 905
Bsp1286I	GDGCHC	1 cut(s)	1000
Bsp1407I	TGTACA	1 cut(s)	465
Bsp143I	GATC	4 cut(s)	403, 506, 775, 909
BspACI	CCGC	2 cut(s)	329, 782
BspANI	GGCC	6 cut(s)	184, 280, 332, 363, 477, 905
BspCNI	CTCAG	2 cut(s)	892, 1079
BspLI	GGNNCC	5 cut(s)	121, 279, 486, 819, 911
BspMAI	CTGCAG	2 cut(s)	165, 602
BspOI	GCTAGC	1 cut(s)	887
BspPI	GGATC	3 cut(s)	514, 904, 917
BspT107I	GGYRCC	1 cut(s)	484
BsrBI	CCGCTC	1 cut(s)	782
BsrGI	TGTACA	1 cut(s)	465
BsrI	ACTGG	2 cut(s)	755, 1256
BssECI	CCNNGG	4 cut(s)	358, 618, 1035, 1291
BssMI	GATC	4 cut(s)	403, 506, 775, 909
BssNAI	GTATAC	1 cut(s)	99
BssT1I	CCWWGG	1 cut(s)	358
Bst1107I	GTATAC	1 cut(s)	99
Bst2UI	CCWGG	4 cut(s)	261, 619, 840, 851
Bst4CI	ACNGT	4 cut(s)	19, 227, 394, 1135
BstAPI	GCANNNNNTGC	2 cut(s)	160, 629
BstAUI	TGTACA	1 cut(s)	465
BstBAI	YACGTR	1 cut(s)	1102
BstC8I	GCNNGC	1 cut(s)	885
BstDEI	CTNAG	2 cut(s)	879, 1066
BstF5I	GGATG	6 cut(s)	615, 859, 1031, 1076, 1222, 1287
BstKTI	GATC	4 cut(s)	406, 509, 778, 912
BstMAI	GTCTC	1 cut(s)	2
BstMBI	GATC	4 cut(s)	403, 506, 775, 909
BstMWI	GCNNNNNNNGC	5 cut(s)	58, 160, 236, 629, 804
BstNI	CCWGG	4 cut(s)	261, 619, 840, 851
BstNSI	RCATGY	2 cut(s)	113, 847
BstPAI	GACNNNNGTC	1 cut(s)	1227
BstSCI	CCNGG	7 cut(s)	259, 617, 838, 849, 905, 1034, 1291
BstSFI	CTRYAG	4 cut(s)	161, 390, 598, 1279
BstV1I	GCAGC	4 cut(s)	45, 163, 226, 1105
BstX2I	RGATCY	2 cut(s)	506, 909
BstYI	RGATCY	2 cut(s)	506, 909
BstZ17I	GTATAC	1 cut(s)	99
Bsu36I	CCTNAGG	1 cut(s)	1066
BsuI	GTATCC	2 cut(s)	564, 740
BsuRI	GGCC	6 cut(s)	184, 280, 332, 363, 477, 905
BtrI	CACGTC	1 cut(s)	1088
BtsCI	GGATG	6 cut(s)	615, 859, 1031, 1076, 1222, 1287
BtsI	GCAGTG	1 cut(s)	198
BtsIMutI	CAGTG	2 cut(s)	198, 495
Cac8I	GCNNGC	1 cut(s)	885
Cfr13I	GGNCC	1 cut(s)	278
CsiI	ACCWGGT	1 cut(s)	849
Csp6I	GTAC	5 cut(s)	20, 40, 107, 466, 847
CviAII	CATG	7 cut(s)	49, 110, 155, 204, 479, 773, 844
CviQI	GTAC	5 cut(s)	20, 40, 107, 466, 847
DdeI	CTNAG	2 cut(s)	879, 1066
DpnI	GATC	4 cut(s)	405, 508, 777, 911
DpnII	GATC	4 cut(s)	403, 506, 775, 909
DraIII	CACNNNGTG	1 cut(s)	1102
EaeI	YGGCCR	2 cut(s)	182, 903
Ecl136II	GAGCTC	1 cut(s)	998
Eco130I	CCWWGG	1 cut(s)	358
Eco24I	GRGCYC	1 cut(s)	1000
Eco32I	GATATC	1 cut(s)	580
Eco53kI	GAGCTC	1 cut(s)	998
Eco57I	CTGAAG	1 cut(s)	230
Eco81I	CCTNAGG	1 cut(s)	1066
EcoICRI	GAGCTC	1 cut(s)	998
EcoRII	CCWGG	4 cut(s)	259, 617, 838, 849
EcoRV	GATATC	1 cut(s)	580
EcoT14I	CCWWGG	1 cut(s)	358
EcoT38I	GRGCYC	1 cut(s)	1000
ErhI	CCWWGG	1 cut(s)	358
FaeI	CATG	7 cut(s)	52, 113, 158, 207, 482, 776, 847
FatI	CATG	7 cut(s)	48, 109, 154, 203, 478, 772, 843
FauI	CCCGC	1 cut(s)	775
FbaI	TGATCA	1 cut(s)	403
FblI	GTMKAC	2 cut(s)	98, 309
Fnu4HI	GCNGC	5 cut(s)	59, 152, 240, 330, 1119
FokI	GGATG	6 cut(s)	622, 846, 1018, 1083, 1229, 1274
FriOI	GRGCYC	1 cut(s)	1000
Fsp4HI	GCNGC	5 cut(s)	59, 152, 240, 330, 1119
FspBI	CTAG	1 cut(s)	884
GluI	GCNGC	5 cut(s)	59, 152, 240, 330, 1119
GsaI	CCCAGC	2 cut(s)	297, 802
GsuI	CTGGAG	1 cut(s)	273
HaeIII	GGCC	6 cut(s)	184, 280, 332, 363, 477, 905
HapII	CCGG	3 cut(s)	906, 1035, 1293
Hin1II	CATG	7 cut(s)	52, 113, 158, 207, 482, 776, 847
HincII	GTYRAC	2 cut(s)	348, 856
HindII	GTYRAC	2 cut(s)	348, 856
HinfI	GANTC	4 cut(s)	298, 500, 680, 1296
HpaII	CCGG	3 cut(s)	906, 1035, 1293
HphI	GGTGA	1 cut(s)	46
Hpy166II	GTNNAC	4 cut(s)	99, 310, 348, 856
Hpy188I	TCNGA	2 cut(s)	963, 1269
Hpy188III	TCNNGA	5 cut(s)	376, 504, 1068, 1129, 1183
Hpy8I	GTNNAC	4 cut(s)	99, 310, 348, 856
HpyAV	CCTTC	4 cut(s)	1064, 1132, 1202, 1264
HpyCH4III	ACNGT	4 cut(s)	19, 227, 394, 1135
HpyCH4IV	ACGT	4 cut(s)	38, 344, 1087, 1101
HpyCH4V	TGCA	9 cut(s)	130, 163, 203, 242, 521, 600, 645, 770, 918
HpyF10VI	GCNNNNNNNGC	5 cut(s)	58, 160, 236, 629, 804
HpyF3I	CTNAG	2 cut(s)	879, 1066
HpySE526I	ACGT	4 cut(s)	38, 344, 1087, 1101
Hsp92II	CATG	7 cut(s)	52, 113, 158, 207, 482, 776, 847
Ksp22I	TGATCA	1 cut(s)	403
Kzo9I	GATC	4 cut(s)	403, 506, 775, 909
LmnI	GCTCC	4 cut(s)	125, 547, 1003, 1238
Lsp1109I	GCAGC	4 cut(s)	45, 163, 226, 1105
LweI	GCATC	1 cut(s)	696
MabI	ACCWGGT	1 cut(s)	849
MaeI	CTAG	1 cut(s)	884
MaeII	ACGT	4 cut(s)	38, 344, 1087, 1101
MaeIII	GTNAC	6 cut(s)	34, 221, 340, 634, 718, 938
MalI	GATC	4 cut(s)	405, 508, 777, 911
MbiI	CCGCTC	1 cut(s)	782
MboI	GATC	4 cut(s)	403, 506, 775, 909
MboII	GAAGA	5 cut(s)	220, 483, 501, 1049, 1117
MflI	RGATCY	2 cut(s)	506, 909
MhlI	GDGCHC	1 cut(s)	1000
MlsI	TGGCCA	1 cut(s)	184
MluCI	AATT	4 cut(s)	84, 525, 561, 956
MluNI	TGGCCA	1 cut(s)	184
MlyI	GAGTC	1 cut(s)	674
MnlI	CCTC	8 cut(s)	84, 141, 229, 268, 440, 733, 1075, 1206
Mox20I	TGGCCA	1 cut(s)	184
MscI	TGGCCA	1 cut(s)	184
MseI	TTAA	1 cut(s)	663
MslI	CAYNNNNRTG	1 cut(s)	114
Msp20I	TGGCCA	1 cut(s)	184
MspA1I	CMGCKG	2 cut(s)	293, 1118
MspI	CCGG	3 cut(s)	906, 1035, 1293
MspR9I	CCNGG	7 cut(s)	261, 619, 840, 851, 907, 1036, 1293
Mva1269I	GAATGC	1 cut(s)	523
MvaI	CCWGG	4 cut(s)	261, 619, 840, 851
MwoI	GCNNNNNNNGC	5 cut(s)	58, 160, 236, 629, 804
NciI	CCSGG	3 cut(s)	907, 1036, 1293
NdeII	GATC	4 cut(s)	403, 506, 775, 909
NheI	GCTAGC	1 cut(s)	883
NlaIII	CATG	7 cut(s)	52, 113, 158, 207, 482, 776, 847
NlaIV	GGNNCC	5 cut(s)	121, 279, 486, 819, 911
NmuCI	GTSAC	4 cut(s)	34, 634, 718, 938
NspI	RCATGY	2 cut(s)	113, 847
PctI	GAATGC	1 cut(s)	523
PfeI	GAWTC	3 cut(s)	298, 500, 1296
Pfl23II	CGTACG	1 cut(s)	39
PflMI	CCANNNNNTGG	2 cut(s)	204, 563
PkrI	GCNGC	5 cut(s)	60, 153, 241, 331, 1120
PleI	GAGTC	1 cut(s)	674
PpsI	GAGTC	1 cut(s)	674
Ppu21I	YACGTR	1 cut(s)	1102
PshAI	GACNNNNGTC	1 cut(s)	1227
Psp124BI	GAGCTC	1 cut(s)	1000
Psp1406I	AACGTT	1 cut(s)	344
Psp6I	CCWGG	4 cut(s)	259, 617, 838, 849
PspFI	CCCAGC	2 cut(s)	293, 798
PspGI	CCWGG	4 cut(s)	259, 617, 838, 849
PspLI	CGTACG	1 cut(s)	39
PspN4I	GGNNCC	5 cut(s)	121, 279, 486, 819, 911
PspPI	GGNCC	1 cut(s)	278
PstI	CTGCAG	2 cut(s)	165, 602
PsuI	RGATCY	2 cut(s)	506, 909
PvuII	CAGCTG	2 cut(s)	293, 1118
RsaI	GTAC	5 cut(s)	21, 41, 108, 467, 848
RsaNI	GTAC	5 cut(s)	20, 40, 107, 466, 847
RseI	CAYNNNNRTG	1 cut(s)	114
SacI	GAGCTC	1 cut(s)	1000
SaqAI	TTAA	1 cut(s)	663
SatI	GCNGC	5 cut(s)	59, 152, 240, 330, 1119
Sau3AI	GATC	4 cut(s)	403, 506, 775, 909
Sau96I	GGNCC	1 cut(s)	278
SchI	GAGTC	1 cut(s)	674
ScrFI	CCNGG	7 cut(s)	261, 619, 840, 851, 907, 1036, 1293
SduI	GDGCHC	1 cut(s)	1000
SexAI	ACCWGGT	1 cut(s)	849
SfaNI	GCATC	1 cut(s)	696
SfcI	CTRYAG	4 cut(s)	161, 390, 598, 1279
SmiMI	CAYNNNNRTG	1 cut(s)	114
SmlI	CTYRAG	1 cut(s)	145
SmoI	CTYRAG	1 cut(s)	145
Sse9I	AATT	4 cut(s)	84, 525, 561, 956
SsiI	CCGC	2 cut(s)	329, 782
SspI	AATATT	1 cut(s)	420
SspMI	CTAG	1 cut(s)	884
SstI	GAGCTC	1 cut(s)	1000
StyD4I	CCNGG	7 cut(s)	259, 617, 838, 849, 905, 1034, 1291
StyI	CCWWGG	1 cut(s)	358
TaaI	ACNGT	4 cut(s)	19, 227, 394, 1135
TaiI	ACGT	4 cut(s)	41, 347, 1090, 1104
TaqI	TCGA	3 cut(s)	301, 576, 778
TaqII	GACCGA	2 cut(s)	1218, 1239
TasI	AATT	4 cut(s)	84, 525, 561, 956
TatI	WGTACW	1 cut(s)	465
TauI	GCSGC	1 cut(s)	332
TfiI	GAWTC	3 cut(s)	298, 500, 1296
Tru1I	TTAA	1 cut(s)	663
Tru9I	TTAA	1 cut(s)	663
TscAI	CASTG	2 cut(s)	205, 502
TseFI	GTSAC	4 cut(s)	34, 634, 718, 938
TseI	GCWGC	4 cut(s)	58, 151, 239, 1118
Tsp45I	GTSAC	4 cut(s)	34, 634, 718, 938
TspDTI	ATGAA	3 cut(s)	436, 672, 1008
TspRI	CASTG	2 cut(s)	205, 502
Van91I	CCANNNNNTGG	2 cut(s)	204, 563
XapI	RAATTY	1 cut(s)	956
XceI	RCATGY	2 cut(s)	113, 847
XmiI	GTMKAC	2 cut(s)	98, 309
XspI	CTAG	1 cut(s)	884

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.