FvH4_6g38370
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016372
Physical Location & Seq
Forward (+)
30322214 .. 30325074
2861 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g38370.t1

Sequence Viewer

Length: 1107 bp
ATGTCAAATGAAAAGAAAAGCCCTTACCTACAGTATGACCCTTTCGATTACAACCCCCACGAAATCGACAGGTCAAGCTTTCCATTCTTCAATTACAGCTCTAGTTCCATATACAATATTCCCCAAACACCAGCAGACCCCCAAACTCAAAGCCTACATGGATTTGAATCTGATCCTTCGTATTTGATGAGCTTCGCCGACTGCTTAAATGGGTCAATGGACTACACCACCCTCTCAAAAGCCTTTGATATTTCTTGTTCATCATCTGAAGTCATTTCTCCGCCGCTGTTAGATCAGGACGATCATTCGAAAAACAAGGCTGCAGCTGCTGTTGTAGGAGATCAAAATCCATCCACACCGAACTCATCGATATCTTGTTCATCTAATGAAGCTGGTGCTGCTCGTCATCATGAGCATGAAAACAATCAAGATTCAGATAAGATCAGCAAGAAAGATAAGCATCAGAAGGTAGTACAGTCAGCTGAAGAAGCTCCTGGAGATCATGAAGACGAGTCAAAGAAAGTGAACAAGGCAAAAAAGAAAGAGAAACGGCAGAGGGAACCGCGGTTCGCGTTCTTGACCAAGAGTGAAATTGATCATCTTGAAGATGGCTACAGATGGAGAAAGTACGGACAGAAGGCAGTCAAGAATAGCCCTTATCCTAGAAGTTATTACAGATGCACTACTCAGAAGTGCAATGTAAAGAAACGTGTGGAGAGATCGTTCCACGATCCGGCGACTGTGATCACAACATATGAAGGGCAGCACAACCATCAATGTCCAGCGACACTTCGAGGCAGTATGAATGCTGTTGGCATGTTGACACCTTCCTTTTTGGGAGGATTCGGGACCAGTAACAACGGATCCCCAAGATTTCCACACCAATTATTGTTAACTCAACTGCTTACTCCAGTCGATAACAACCAACTTCAACTGCAAGCTCAACATCATTATGATCCAGCAGGTTCTATTTTCTACTCAAATCTCATGAGTACTCTTCATCAGAATCAACCGCAGCAGCAGCAGCAGCATGTACATGTTCCTCATGACTACAATTTGTTGCAAGATTTAGCTCCCTCATTTAGTCACAAGCTGGAGGAGCCATGA

Protein Analysis

369

Amino Acids

41.62

Weight (kDa)

6.46

Isoelectric Point (pI)

62.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 202 - 259 2.8e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016372)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g38370
malus_domestica MD09G1150700.v1.1 MD17G1138100.v1.1
prunus_persica Prupe.3G174300_v2.0.a1
pyrus_communis pycom09g07040
rosa_chinensis RchiOBHm_Chr2g0151681
rosa_laevigata RLG00000020568
rosa_multiflora Rmu_sc0005294.1_g000047
rosa_roxburghii Rroxscaffold_2G00096260
rosa_rugosa Rorug02G0428000
rosa_samantha Rh2AG488700 Rh2BG501500 Rh2CG475300 Rh2DG512600
rosa_wichuraiana Rw2G040080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 953
AccII CGCG 2 cut(s) 565, 572
AciI CCGC 5 cut(s) 281, 284, 563, 565, 1013
AclWI GGATC 5 cut(s) 167, 725, 858, 871, 950
AcuI CTGAAG 2 cut(s) 288, 504
AfaI GTAC 4 cut(s) 474, 629, 994, 1035
AfiI CCNNNNNNNGG 1 cut(s) 733
AflIII ACRYGT 2 cut(s) 709, 1036
AgsI TTSAA 4 cut(s) 91, 167, 605, 932
AjnI CCWGG 1 cut(s) 493
AloI GAACNNNNNNTCC 4 cut(s) 551, 583, 707, 739
AlwI GGATC 5 cut(s) 167, 725, 858, 871, 950
AlwNI CAGNNNCTG 1 cut(s) 329
AspS9I GGNCC 1 cut(s) 849
AsuII TTCGAA 1 cut(s) 308
AvaII GGWCC 1 cut(s) 849
BamHI GGATCC 1 cut(s) 863
BbsI GAAGAC 1 cut(s) 513
BccI CCATC 4 cut(s) 358, 602, 612, 780
BceAI ACGGC 1 cut(s) 566
BciT130I CCWGG 1 cut(s) 495
BclI TGATCA 2 cut(s) 595, 744
BfaI CTAG 2 cut(s) 102, 663
BfmI CTRYAG 3 cut(s) 29, 321, 613
BfuAI ACCTGC 1 cut(s) 953
BmcAI AGTACT 1 cut(s) 994
Bme1390I CCNGG 1 cut(s) 495
Bme18I GGWCC 1 cut(s) 849
BmgT120I GGNCC 1 cut(s) 849
BmiI GGNNCC 4 cut(s) 561, 850, 865, 1101
BmrFI CCNGG 1 cut(s) 495
BmsI GCATC 2 cut(s) 469, 668
BpiI GAAGAC 1 cut(s) 513
BpmI CTGGAG 2 cut(s) 516, 894
Bpu14I TTCGAA 1 cut(s) 308
Bsa29I ATCGAT 1 cut(s) 368
BsaBI GATNNNNATC 3 cut(s) 166, 345, 459
BsaJI CCNNGG 1 cut(s) 563
BsaXI ACNNNNNCTCC 2 cut(s) 707, 737
Bsc4I CCNNNNNNNGG 1 cut(s) 733
Bse1I ACTGG 2 cut(s) 852, 911
Bse3DI GCAATG 1 cut(s) 703
Bse8I GATNNNNATC 3 cut(s) 166, 345, 459
BseBI CCWGG 1 cut(s) 495
BseCI ATCGAT 1 cut(s) 368
BseDI CCNNGG 1 cut(s) 563
BseGI GGATG 1 cut(s) 350
BseJI GATNNNNATC 3 cut(s) 166, 345, 459
BseLI CCNNNNNNNGG 1 cut(s) 733
BseMI GCAATG 1 cut(s) 703
BseMII CTCAG 1 cut(s) 701
BseNI ACTGG 2 cut(s) 852, 911
Bsh1236I CGCG 2 cut(s) 565, 572
BshVI ATCGAT 1 cut(s) 368
BsiSI CCGG 1 cut(s) 734
BslFI GGGAC 1 cut(s) 862
BslI CCNNNNNNNGG 1 cut(s) 733
BsmFI GGGAC 1 cut(s) 862
BsmI GAATGC 1 cut(s) 811
Bsp119I TTCGAA 1 cut(s) 308
Bsp1407I TGTACA 1 cut(s) 1033
BspACI CCGC 5 cut(s) 281, 284, 563, 565, 1013
BspCNI CTCAG 1 cut(s) 700
BspDI ATCGAT 1 cut(s) 368
BspFNI CGCG 2 cut(s) 565, 572
BspHI TCATGA 4 cut(s) 409, 502, 987, 1045
BspLI GGNNCC 4 cut(s) 561, 850, 865, 1101
BspMAI CTGCAG 1 cut(s) 325
BspMI ACCTGC 1 cut(s) 953
BspPI GGATC 5 cut(s) 167, 725, 858, 871, 950
BspT104I TTCGAA 1 cut(s) 308
BsrDI GCAATG 1 cut(s) 703
BsrGI TGTACA 1 cut(s) 1033
BsrI ACTGG 2 cut(s) 852, 911
BssECI CCNNGG 1 cut(s) 563
Bst2UI CCWGG 1 cut(s) 495
Bst4CI ACNGT 3 cut(s) 33, 477, 742
Bst6I CTCTTC 1 cut(s) 1002
BstAUI TGTACA 1 cut(s) 1033
BstBI TTCGAA 1 cut(s) 308
BstC8I GCNNGC 1 cut(s) 939
BstDEI CTNAG 1 cut(s) 687
BstDSI CCRYGG 1 cut(s) 563
BstF5I GGATG 1 cut(s) 350
BstFNI CGCG 2 cut(s) 565, 572
BstMWI GCNNNNNNNGC 7 cut(s) 326, 398, 488, 1021, 1024, 1027, 1099
BstNI CCWGG 1 cut(s) 495
BstNSI RCATGY 3 cut(s) 820, 1034, 1040
BstSCI CCNGG 1 cut(s) 493
BstSFI CTRYAG 3 cut(s) 29, 321, 613
BstUI CGCG 2 cut(s) 565, 572
BstV2I GAAGAC 1 cut(s) 513
BstX2I RGATCY 1 cut(s) 863
BstYI RGATCY 1 cut(s) 863
Bsu15I ATCGAT 1 cut(s) 368
BsuTUI ATCGAT 1 cut(s) 368
BtgI CCRYGG 1 cut(s) 563
BtsCI GGATG 1 cut(s) 350
BveI ACCTGC 1 cut(s) 953
Cac8I GCNNGC 1 cut(s) 939
CaiI CAGNNNCTG 1 cut(s) 329
CciI TCATGA 4 cut(s) 409, 502, 987, 1045
Cfr13I GGNCC 1 cut(s) 849
Cfr42I CCGCGG 1 cut(s) 566
ClaI ATCGAT 1 cut(s) 368
Csp6I GTAC 4 cut(s) 473, 628, 993, 1034
CviQI GTAC 4 cut(s) 473, 628, 993, 1034
DdeI CTNAG 1 cut(s) 687
Eam1104I CTCTTC 1 cut(s) 1002
EarI CTCTTC 1 cut(s) 1002
EciI GGCGGA 1 cut(s) 270
Eco32I GATATC 1 cut(s) 372
Eco47I GGWCC 1 cut(s) 849
Eco57I CTGAAG 2 cut(s) 288, 504
EcoRII CCWGG 1 cut(s) 493
EcoRV GATATC 1 cut(s) 372
FaqI GGGAC 1 cut(s) 862
FauNDI CATATG 1 cut(s) 754
FbaI TGATCA 2 cut(s) 595, 744
FokI GGATG 1 cut(s) 337
FspBI CTAG 2 cut(s) 102, 663
GsuI CTGGAG 2 cut(s) 516, 894
HapII CCGG 1 cut(s) 734
HincII GTYRAC 2 cut(s) 822, 894
HindII GTYRAC 2 cut(s) 822, 894
HindIII AAGCTT 1 cut(s) 76
HinfI GANTC 5 cut(s) 167, 431, 512, 843, 1006
HpaI GTTAAC 1 cut(s) 894
HpaII CCGG 1 cut(s) 734
Hpy166II GTNNAC 3 cut(s) 526, 822, 894
Hpy188I TCNGA 6 cut(s) 172, 268, 436, 465, 690, 1005
Hpy8I GTNNAC 3 cut(s) 526, 822, 894
HpyAV CCTTC 5 cut(s) 186, 460, 631, 752, 837
HpyCH4III ACNGT 3 cut(s) 33, 477, 742
HpyCH4IV ACGT 1 cut(s) 709
HpyCH4V TGCA 5 cut(s) 323, 681, 696, 937, 1063
HpyF10VI GCNNNNNNNGC 7 cut(s) 326, 398, 488, 1021, 1024, 1027, 1099
HpyF3I CTNAG 1 cut(s) 687
HpySE526I ACGT 1 cut(s) 709
Ksp22I TGATCA 2 cut(s) 595, 744
KspAI GTTAAC 1 cut(s) 894
KspI CCGCGG 1 cut(s) 566
LmnI GCTCC 3 cut(s) 496, 1078, 1099
LweI GCATC 2 cut(s) 469, 668
MaeI CTAG 2 cut(s) 102, 663
MaeII ACGT 1 cut(s) 709
MaeIII GTNAC 2 cut(s) 854, 1085
MboII GAAGA 5 cut(s) 79, 497, 518, 617, 989
MflI RGATCY 1 cut(s) 863
MluCI AATT 4 cut(s) 91, 591, 884, 1054
MlyI GAGTC 1 cut(s) 521
MnlI CCTC 7 cut(s) 242, 549, 788, 833, 1053, 1087, 1090
MseI TTAA 2 cut(s) 206, 893
MslI CAYNNNNRTG 3 cut(s) 414, 951, 1035
MspA1I CMGCKG 4 cut(s) 286, 326, 482, 565
MspI CCGG 1 cut(s) 734
MspR9I CCNGG 1 cut(s) 495
Mva1269I GAATGC 1 cut(s) 811
MvaI CCWGG 1 cut(s) 495
MvnI CGCG 2 cut(s) 565, 572
MwoI GCNNNNNNNGC 7 cut(s) 326, 398, 488, 1021, 1024, 1027, 1099
NdeI CATATG 1 cut(s) 754
NlaIV GGNNCC 4 cut(s) 561, 850, 865, 1101
NmuCI GTSAC 1 cut(s) 1085
NspI RCATGY 3 cut(s) 820, 1034, 1040
NspV TTCGAA 1 cut(s) 308
PagI TCATGA 4 cut(s) 409, 502, 987, 1045
PciI ACATGT 1 cut(s) 1036
PctI GAATGC 1 cut(s) 811
PfeI GAWTC 4 cut(s) 167, 431, 843, 1006
PfoI TCCNGGA 1 cut(s) 493
PleI GAGTC 1 cut(s) 520
PpsI GAGTC 1 cut(s) 520
PscI ACATGT 1 cut(s) 1036
Psp6I CCWGG 1 cut(s) 493
PspGI CCWGG 1 cut(s) 493
PspN4I GGNNCC 4 cut(s) 561, 850, 865, 1101
PspPI GGNCC 1 cut(s) 849
PstI CTGCAG 1 cut(s) 325
PstNI CAGNNNCTG 1 cut(s) 329
PsuI RGATCY 1 cut(s) 863
PvuII CAGCTG 2 cut(s) 326, 482
RsaI GTAC 4 cut(s) 474, 629, 994, 1035
RsaNI GTAC 4 cut(s) 473, 628, 993, 1034
RseI CAYNNNNRTG 3 cut(s) 414, 951, 1035
SacII CCGCGG 1 cut(s) 566
SaqAI TTAA 2 cut(s) 206, 893
Sau96I GGNCC 1 cut(s) 849
ScaI AGTACT 1 cut(s) 994
SchI GAGTC 1 cut(s) 521
ScrFI CCNGG 1 cut(s) 495
SfaNI GCATC 2 cut(s) 469, 668
SfcI CTRYAG 3 cut(s) 29, 321, 613
Sfr303I CCGCGG 1 cut(s) 566
SfuI TTCGAA 1 cut(s) 308
SgrBI CCGCGG 1 cut(s) 566
SinI GGWCC 1 cut(s) 849
SmiMI CAYNNNNRTG 3 cut(s) 414, 951, 1035
Sse9I AATT 4 cut(s) 91, 591, 884, 1054
SsiI CCGC 5 cut(s) 281, 284, 563, 565, 1013
SspI AATATT 1 cut(s) 118
SspMI CTAG 2 cut(s) 102, 663
StyD4I CCNGG 1 cut(s) 493
TaaI ACNGT 3 cut(s) 33, 477, 742
TaiI ACGT 1 cut(s) 712
TaqI TCGA 6 cut(s) 45, 66, 308, 368, 793, 915
TasI AATT 4 cut(s) 91, 591, 884, 1054
TatI WGTACW 3 cut(s) 472, 992, 1033
TauI GCSGC 1 cut(s) 286
TfiI GAWTC 4 cut(s) 167, 431, 843, 1006
Tru1I TTAA 2 cut(s) 206, 893
Tru9I TTAA 2 cut(s) 206, 893
TseFI GTSAC 1 cut(s) 1085
Tsp45I GTSAC 1 cut(s) 1085
TspDTI ATGAA 9 cut(s) 24, 249, 369, 402, 432, 519, 771, 818, 989
TspGWI ACGGA 2 cut(s) 645, 876
VpaK11BI GGWCC 1 cut(s) 849
XceI RCATGY 3 cut(s) 820, 1034, 1040
XspI CTAG 2 cut(s) 102, 663
ZrmI AGTACT 1 cut(s) 994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.